DE112023001402T5 - Modifizierte proteinglutaminase - Google Patents

Modifizierte proteinglutaminase Download PDF

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Publication number
DE112023001402T5
DE112023001402T5 DE112023001402.7T DE112023001402T DE112023001402T5 DE 112023001402 T5 DE112023001402 T5 DE 112023001402T5 DE 112023001402 T DE112023001402 T DE 112023001402T DE 112023001402 T5 DE112023001402 T5 DE 112023001402T5
Authority
DE
Germany
Prior art keywords
amino acid
residue
substitution
seq
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
DE112023001402.7T
Other languages
German (de)
English (en)
Inventor
Atsushi Ono
Kazunori Yoshida
Ryutaro Ariyoshi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amano Enzyme Inc
Original Assignee
Amano Enzyme Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amano Enzyme Inc filed Critical Amano Enzyme Inc
Publication of DE112023001402T5 publication Critical patent/DE112023001402T5/de
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01002—Glutaminase (3.5.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01044—Protein-glutamine glutaminase (3.5.1.44)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DE112023001402.7T 2022-03-16 2023-03-16 Modifizierte proteinglutaminase Pending DE112023001402T5 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2022041629 2022-03-16
JP2022-041629 2022-03-16
JP2022-084393 2022-05-24
JP2022084393 2022-05-24
PCT/JP2023/010448 WO2023176943A1 (ja) 2022-03-16 2023-03-16 改変型プロテイングルタミナーゼ

Publications (1)

Publication Number Publication Date
DE112023001402T5 true DE112023001402T5 (de) 2025-01-02

Family

ID=88023418

Family Applications (1)

Application Number Title Priority Date Filing Date
DE112023001402.7T Pending DE112023001402T5 (de) 2022-03-16 2023-03-16 Modifizierte proteinglutaminase

Country Status (5)

Country Link
US (1) US20250188436A1 (https=)
EP (1) EP4495244A1 (https=)
JP (1) JPWO2023176943A1 (https=)
DE (1) DE112023001402T5 (https=)
WO (1) WO2023176943A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025140303A1 (en) * 2023-12-28 2025-07-03 Novozymes A/S Enzymatic treatment of a material comprising corn protein
WO2025239456A1 (ja) * 2024-05-17 2025-11-20 天野エンザイム株式会社 複合型プロテイングルタミナーゼ

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3609648B2 (ja) * 1998-06-04 2005-01-12 天野エンザイム株式会社 新規蛋白質脱アミド酵素、それをコードする遺伝子、その製造法並びにその用途
JP3696500B2 (ja) * 1999-12-03 2005-09-21 天野エンザイム株式会社 新規蛋白質脱アミド酵素、それを生産する微生物、それをコードする遺伝子、その製造法及び用途
BRPI0811492B8 (pt) * 2007-05-11 2018-05-08 Chr Hansen As método para produzir uma bebida láctea acidificada, bebida láctea acidificada, e, polipeptídeo isolado com atividade desamidase
DK2351837T3 (da) * 2008-09-09 2019-05-13 Amano Enzyme Inc Fremgangsmåde til at designe mutant enzym, fremgangsmåde til at fremstille mutant enzym og mutant enzym

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
A novel protein-deamidating enzyme from Chryseobacterium proteolyticum sp. nov., a newly isolated bacterium from soil. Applied and Environmental Microbiology 2000; 66(8): 3337-43
Sanger et al. (1977), Proc. Natl. Acad. Sci. USA 74: 5463
Tatiana A. Tatsusova, Thomas L. Madden, FEMS Microbiol. Lett., Band 174, Seiten 247-250, 1999

Also Published As

Publication number Publication date
EP4495244A1 (en) 2025-01-22
US20250188436A1 (en) 2025-06-12
JPWO2023176943A1 (https=) 2023-09-21
WO2023176943A1 (ja) 2023-09-21

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Representative=s name: VOSSIUS & PARTNER PATENTANWAELTE RECHTSANWAELT, DE