DE102017125335B4 - Amplikon-Region als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere nicht-klassischer Monozyten - Google Patents

Amplikon-Region als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere nicht-klassischer Monozyten Download PDF

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DE102017125335B4
DE102017125335B4 DE102017125335.1A DE102017125335A DE102017125335B4 DE 102017125335 B4 DE102017125335 B4 DE 102017125335B4 DE 102017125335 A DE102017125335 A DE 102017125335A DE 102017125335 B4 DE102017125335 B4 DE 102017125335B4
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amplicon
seq
cells
methylation
cpg
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DE102017125335A1 (de
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Sven Olek
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Precision for Medicine GmbH
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Epiontis GmbH
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Priority to DE102017125335.1A priority Critical patent/DE102017125335B4/de
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Priority to PCT/EP2018/079406 priority patent/WO2019081707A1/en
Priority to JP2020523795A priority patent/JP2021502806A/ja
Priority to CA3079477A priority patent/CA3079477A1/en
Priority to CN201880068476.5A priority patent/CN111247253A/zh
Priority to AU2018356572A priority patent/AU2018356572A1/en
Priority to EP18793655.4A priority patent/EP3669001B1/en
Priority to US16/758,992 priority patent/US11680294B2/en
Publication of DE102017125335A1 publication Critical patent/DE102017125335A1/de
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/154Methylation markers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
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  • Molecular Biology (AREA)
  • Biophysics (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DE102017125335.1A 2017-10-27 2017-10-27 Amplikon-Region als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere nicht-klassischer Monozyten Active DE102017125335B4 (de)

Priority Applications (8)

Application Number Priority Date Filing Date Title
DE102017125335.1A DE102017125335B4 (de) 2017-10-27 2017-10-27 Amplikon-Region als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere nicht-klassischer Monozyten
JP2020523795A JP2021502806A (ja) 2017-10-27 2018-10-26 免疫細胞、特に非古典的単球の同定のためのエピジェネティックマーカーとしての単位複製配列領域
CA3079477A CA3079477A1 (en) 2017-10-27 2018-10-26 Amplicon region as epigenetic marker for the identification of non-classical monocytes
CN201880068476.5A CN111247253A (zh) 2017-10-27 2018-10-26 作为用于鉴定免疫细胞尤其是非典型单核细胞的表观遗传标记的扩增子区
PCT/EP2018/079406 WO2019081707A1 (en) 2017-10-27 2018-10-26 AMPLICON REGION AS AN EPIGENETIC MARKER FOR THE IDENTIFICATION OF NON-CLASSIC MONOCYTES
AU2018356572A AU2018356572A1 (en) 2017-10-27 2018-10-26 Amplicon region as epigenetic marker for the identification of non-classical monocytes
EP18793655.4A EP3669001B1 (en) 2017-10-27 2018-10-26 Amplicon region as epigenetic marker for the identification of non-classical monocytes
US16/758,992 US11680294B2 (en) 2017-10-27 2018-10-26 Amplicon region as epigenetic marker for the identification of non-classical monocytes

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DE102017125335.1A DE102017125335B4 (de) 2017-10-27 2017-10-27 Amplikon-Region als epigenetischer Marker zur Identifizierung von Immunzellen, insbesondere nicht-klassischer Monozyten

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DE102017125335B4 true DE102017125335B4 (de) 2019-10-17

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EP (1) EP3669001B1 (enExample)
JP (1) JP2021502806A (enExample)
CN (1) CN111247253A (enExample)
AU (1) AU2018356572A1 (enExample)
CA (1) CA3079477A1 (enExample)
DE (1) DE102017125335B4 (enExample)
WO (1) WO2019081707A1 (enExample)

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WO2022191293A1 (ja) * 2021-03-12 2022-09-15 エーザイ・アール・アンド・ディー・マネジメント株式会社 全単球及びcd16陽性単球の割合の測定方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012162660A2 (en) 2011-05-25 2012-11-29 Brown University Methods using dna methylation for identifying a cell or a mixture of cells for prognosis and diagnosis of diseases, and for cell remediation therapies

Family Cites Families (3)

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CN107941681B (zh) * 2013-04-19 2021-07-30 艾皮恩蒂斯有限公司 鉴定生物样品中定量细胞组成的方法
BR112015029299A2 (pt) * 2013-05-22 2017-07-25 Yeda Res & Dev subpopulação de monócitos humanos para tratamento de doenças e distúrbios oculares
GB201516975D0 (en) * 2015-09-25 2015-11-11 Epiontis Gmbh PARK2 as epigenetic marker for the identification of immune cells, in particular monocytes

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012162660A2 (en) 2011-05-25 2012-11-29 Brown University Methods using dna methylation for identifying a cell or a mixture of cells for prognosis and diagnosis of diseases, and for cell remediation therapies

Non-Patent Citations (10)

* Cited by examiner, † Cited by third party
Title
Accomando et al. (in: Accomando et al. Quantitative reconstruction of leukocyte subsets using DNA methylation. Genome Biol. 2014 Mar 5;15(3)
Antequera und Bird, Proc Natl Acad Sci USA 90: 11995-9, 1993
Esteller, Oncogene 21:5427-5440, 2002
Illingworth et al. (in Illingworth et al. A novel CpG island set identifies tissue-specific methylation at developmental gene loci. PLoS Biol. 2008 Jan;6(1):e22. doi: 10.1371/journal.pbio.0060022)
Jones und Laird, Nature Genetics 21:163-167, 1999
Kristensen und Hansen PCR-Based Methods for Detecting Single-Locus DNA Methylation Biomarkers in Cancer Diagnostics, Prognostics, and Response to Treatment Clinical Chemistry 55:8 1471-1483 (2009)
Laird, Nature Reviews/Cancer 3:253-266, 2003
LIU, Y. [u.a.]: Blood monocyte transcriptome and epigenome analyses reveal loci associated with human atherosclerosis. Nat. Commun. (elektronisch veröffentlicht 30.08.2017) 8 (1):393, Seiten 1-12 *
Michael J. Booth et al. Quantitative Sequencing of 5-Methylcytosine and 5-Hydroxymethylcytosine at Single-Base Resolution Science 18 May 2012, Vol. 336 no. 6083 pp. 934-937
Zawada et al. (in: Zawada et al. DNA methylation profiling reveals differences in the 3 human monocyte subsets and identifies uremia to induce DNA methylation changes during differentiation. Epigenetics. 2016 Apr 2;11(4):259-72

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CA3079477A1 (en) 2019-05-02
US20200340053A1 (en) 2020-10-29
AU2018356572A1 (en) 2020-04-23
CN111247253A (zh) 2020-06-05
JP2021502806A (ja) 2021-02-04
EP3669001B1 (en) 2024-07-17
US11680294B2 (en) 2023-06-20
DE102017125335A1 (de) 2019-05-02
WO2019081707A1 (en) 2019-05-02
EP3669001A1 (en) 2020-06-24

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