CZ397998A3 - Způsob sekvenční analýzy ligací kódovaného adaptoru - Google Patents

Způsob sekvenční analýzy ligací kódovaného adaptoru Download PDF

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Publication number
CZ397998A3
CZ397998A3 CZ983979A CZ397998A CZ397998A3 CZ 397998 A3 CZ397998 A3 CZ 397998A3 CZ 983979 A CZ983979 A CZ 983979A CZ 397998 A CZ397998 A CZ 397998A CZ 397998 A3 CZ397998 A3 CZ 397998A3
Authority
CZ
Czechia
Prior art keywords
tag
encoded
tags
nucleotides
polynucleotide
Prior art date
Application number
CZ983979A
Other languages
Czech (cs)
English (en)
Inventor
Glenn Albrecht
Sydney Brenner
David H. Lloyd
Robert B. Dubridge
Michael C. Pallas
Original Assignee
Lynx Therapeutics, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/659,453 external-priority patent/US5846719A/en
Application filed by Lynx Therapeutics, Inc. filed Critical Lynx Therapeutics, Inc.
Publication of CZ397998A3 publication Critical patent/CZ397998A3/cs

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
CZ983979A 1996-06-06 1997-06-02 Způsob sekvenční analýzy ligací kódovaného adaptoru CZ397998A3 (cs)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/659,453 US5846719A (en) 1994-10-13 1996-06-06 Oligonucleotide tags for sorting and identification
US68958796A 1996-08-12 1996-08-12

Publications (1)

Publication Number Publication Date
CZ397998A3 true CZ397998A3 (cs) 1999-07-14

Family

ID=27097828

Family Applications (1)

Application Number Title Priority Date Filing Date
CZ983979A CZ397998A3 (cs) 1996-06-06 1997-06-02 Způsob sekvenční analýzy ligací kódovaného adaptoru

Country Status (12)

Country Link
EP (1) EP0923650B1 (ref)
JP (1) JP4124377B2 (ref)
CN (1) CN1195872C (ref)
AT (1) ATE356221T1 (ref)
AU (1) AU733782B2 (ref)
CA (1) CA2256700A1 (ref)
CZ (1) CZ397998A3 (ref)
DE (1) DE69737450T2 (ref)
HU (1) HUP0003944A3 (ref)
NO (1) NO985698L (ref)
PL (1) PL331513A1 (ref)
WO (1) WO1997046704A1 (ref)

Families Citing this family (91)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE43097E1 (en) 1994-10-13 2012-01-10 Illumina, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
ATE466109T1 (de) * 1995-06-07 2010-05-15 Solexa Inc Verfahren zur verbesserung der effizienz der polynukleotidsequenzierung
GB9620769D0 (en) * 1996-10-04 1996-11-20 Brax Genomics Ltd Nucleic acid sequencing
US5888737A (en) * 1997-04-15 1999-03-30 Lynx Therapeutics, Inc. Adaptor-based sequence analysis
EP0985142A4 (en) 1997-05-23 2006-09-13 Lynx Therapeutics Inc SYSTEM AND APPARATUS FOR THE SEQUENTIAL TREATMENT OF ANALYTES
US6607878B2 (en) * 1997-10-06 2003-08-19 Stratagene Collections of uniquely tagged molecules
US6265163B1 (en) 1998-01-09 2001-07-24 Lynx Therapeutics, Inc. Solid phase selection of differentially expressed genes
JP3944996B2 (ja) 1998-03-05 2007-07-18 株式会社日立製作所 Ｄｎａプローブアレー
US7060431B2 (en) 1998-06-24 2006-06-13 Illumina, Inc. Method of making and decoding of array sensors with microspheres
US6480791B1 (en) * 1998-10-28 2002-11-12 Michael P. Strathmann Parallel methods for genomic analysis
WO2000039333A1 (en) * 1998-12-23 2000-07-06 Jones Elizabeth Louise Sequencing method using magnifying tags
NO986133D0 (no) * 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
US6261782B1 (en) * 1999-04-06 2001-07-17 Yale University Fixed address analysis of sequence tags
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
EP2360270B1 (en) 1999-05-20 2016-11-09 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
US8080380B2 (en) 1999-05-21 2011-12-20 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
US8481268B2 (en) 1999-05-21 2013-07-09 Illumina, Inc. Use of microfluidic systems in the detection of target analytes using microsphere arrays
JP2003527087A (ja) * 1999-08-13 2003-09-16 イェール・ユニバーシティ バイナリーコード化配列タグ
ATE542916T1 (de) 1999-08-18 2012-02-15 Illumina Inc Methoden zur erzeugung von oligonukleotidlösungen
AU3806701A (en) 2000-02-07 2001-08-14 Illumina Inc Nucleic acid detection methods using universal priming
AU2001238068A1 (en) 2000-02-07 2001-08-14 Illumina, Inc. Nucleic acid detection methods using universal priming
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7361488B2 (en) 2000-02-07 2008-04-22 Illumina, Inc. Nucleic acid detection methods using universal priming
ATE412774T1 (de) 2000-02-16 2008-11-15 Illumina Inc Parallele genotypisierung mehrerer patientenproben
WO2003102216A2 (en) * 2002-05-31 2003-12-11 Diversa Corporation Multiplexed systems for nucleic acid sequencing
CA2513985C (en) 2003-01-21 2012-05-29 Illumina Inc. Chemical reaction monitor
CA2513889A1 (en) * 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
GB0308852D0 (en) * 2003-04-16 2003-05-21 Lingvitae As Method
WO2005010184A1 (ja) * 2003-07-25 2005-02-03 Takara Bio Inc. 変異の検出方法
GB0324456D0 (en) 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US7622281B2 (en) 2004-05-20 2009-11-24 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for clonal amplification of nucleic acid
EP2272983A1 (en) 2005-02-01 2011-01-12 AB Advanced Genetic Analysis Corporation Reagents, methods and libraries for bead-based sequencing
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
EP1987159B2 (en) * 2006-02-08 2020-08-12 Illumina Cambridge Limited Method for sequencing a polynucleotide template
WO2007097443A1 (ja) * 2006-02-20 2007-08-30 National University Corporation Hokkaido University Ｄｎａ塩基配列を決定する方法
JP2009538123A (ja) * 2006-04-19 2009-11-05 アプライド バイオシステムズ， エルエルシー ゲル非含有ビーズベースの配列決定のための試薬、方法およびライブラリー
US8889348B2 (en) 2006-06-07 2014-11-18 The Trustees Of Columbia University In The City Of New York DNA sequencing by nanopore using modified nucleotides
US9605307B2 (en) 2010-02-08 2017-03-28 Genia Technologies, Inc. Systems and methods for forming a nanopore in a lipid bilayer
US9678055B2 (en) 2010-02-08 2017-06-13 Genia Technologies, Inc. Methods for forming a nanopore in a lipid bilayer
US8324914B2 (en) 2010-02-08 2012-12-04 Genia Technologies, Inc. Systems and methods for characterizing a molecule
WO2012088339A2 (en) 2010-12-22 2012-06-28 Genia Technologies, Inc. Nanopore-based single dna molecule characterization using speed bumps
US8962242B2 (en) 2011-01-24 2015-02-24 Genia Technologies, Inc. System for detecting electrical properties of a molecular complex
US9110478B2 (en) 2011-01-27 2015-08-18 Genia Technologies, Inc. Temperature regulation of measurement arrays
US9074204B2 (en) * 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
EP3604555B1 (en) * 2011-10-14 2024-12-25 President and Fellows of Harvard College Sequencing by structure assembly
EP4491741B1 (en) 2011-12-22 2025-10-15 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
ES2991004T3 (es) 2011-12-22 2024-12-02 Harvard College Métodos para la detección de analitos
US8986629B2 (en) 2012-02-27 2015-03-24 Genia Technologies, Inc. Sensor circuit for controlling, detecting, and measuring a molecular complex
CN102766689B (zh) * 2012-04-17 2015-07-22 盛司潼 一种增加测序读长的测序方法
EP2841601B1 (en) * 2012-04-24 2019-03-06 Gen9, Inc. Methods for sorting nucleic acids and multiplexed preparative in vitro cloning
EP2847353B1 (en) * 2012-05-10 2022-01-19 The General Hospital Corporation Methods for determining a nucleotide sequence
US9914967B2 (en) 2012-06-05 2018-03-13 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using DNA origami probes
GB2510719A (en) 2012-06-15 2014-08-13 Genia Technologies Inc Chip set-up and high-accuracy nucleic acid sequencing
US9605309B2 (en) 2012-11-09 2017-03-28 Genia Technologies, Inc. Nucleic acid sequencing using tags
US9759711B2 (en) 2013-02-05 2017-09-12 Genia Technologies, Inc. Nanopore arrays
US10648026B2 (en) 2013-03-15 2020-05-12 The Trustees Of Columbia University In The City Of New York Raman cluster tagged molecules for biological imaging
SG10201710030QA (en) 2013-06-04 2018-01-30 Harvard College Rna-guided transcriptional regulation
ES2717756T3 (es) * 2013-09-30 2019-06-25 Qiagen Gmbh Moléculas adaptadoras de ADN para la preparación de genotecas de ADN y método para su producción y uso
US9551697B2 (en) 2013-10-17 2017-01-24 Genia Technologies, Inc. Non-faradaic, capacitively coupled measurement in a nanopore cell array
US9322062B2 (en) 2013-10-23 2016-04-26 Genia Technologies, Inc. Process for biosensor well formation
EP3640349A3 (en) 2013-10-23 2020-07-29 Roche Sequencing Solutions, Inc. High speed molecular sensing with nanopores
SG11201605775UA (en) * 2014-01-28 2016-08-30 Lanzatech New Zealand Ltd Method of producing a recombinant microorganism
DK3604544T3 (en) * 2014-01-31 2022-02-14 Swift Biosciences Inc Improved methods for processing dna substrates
US10179932B2 (en) 2014-07-11 2019-01-15 President And Fellows Of Harvard College Methods for high-throughput labelling and detection of biological features in situ using microscopy
CN106661636A (zh) * 2014-09-05 2017-05-10 凯杰有限公司 衔接子连接的扩增子的制备
CN107075513B (zh) 2014-09-12 2020-11-03 深圳华大智造科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
EP3207169B1 (en) * 2014-10-14 2020-07-01 MGI Tech Co., Ltd. Mate pair library construction
CN106661575B (zh) * 2014-10-14 2020-03-24 深圳华大智造科技有限公司 一种接头元件和使用其构建测序文库的方法
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10689690B2 (en) * 2015-08-13 2020-06-23 Centrillion Technology Holdings Corporation Library construction using Y-adapters and vanishing restriction sites
WO2017079406A1 (en) 2015-11-03 2017-05-11 President And Fellows Of Harvard College Method and apparatus for volumetric imaging of a three-dimensional nucleic acid containing matrix
CN108603224B (zh) 2015-12-16 2022-06-28 富鲁达公司 高水平多路复用扩增
WO2017156336A1 (en) 2016-03-10 2017-09-14 The Board Of Trustees Of The Leland Stanford Junior University Transposase-mediated imaging of the accessible genome
AU2017257624B2 (en) 2016-04-25 2023-05-25 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
CA3030430A1 (en) 2016-07-15 2018-01-18 The Regents Of The University Of California Methods of producing nucleic acid libraries
JP7239465B2 (ja) 2016-08-31 2023-03-14 プレジデント アンド フェローズ オブ ハーバード カレッジ 蛍光ｉｎ ｓｉｔｕ配列決定による検出のための核酸配列ライブラリの作製法
GB2569252A (en) 2016-08-31 2019-06-12 Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
US10550425B2 (en) * 2016-11-14 2020-02-04 Agilent Technologies, Inc. Composition and method for improving signal-to-noise in hybridization to oligonucleotide arrays
WO2018102064A1 (en) 2016-11-30 2018-06-07 Microsoft Technology Licensing, Llc. Dna random access storage system via ligation
US11185568B2 (en) 2017-04-14 2021-11-30 President And Fellows Of Harvard College Methods for generation of cell-derived microfilament network
CN107299151B (zh) * 2017-08-28 2020-06-09 华中农业大学 用于鉴别稻米脂肪酸品质的dna分子标记及其应用
EP3737774B1 (en) 2018-01-12 2024-12-25 Claret Bioscience, LLC Method for analyzing nucleic acid
EP3749740B1 (en) 2018-02-05 2023-08-30 The Board Of Trustees Of The Leland Stanford Junior University Systems and methods for multiplexed measurements in single and ensemble cells
AU2019280712B2 (en) 2018-06-06 2025-11-20 The Regents Of The University Of California Methods of producing nucleic acid libraries and compositions and kits for practicing same
EP3836967A4 (en) 2018-07-30 2022-06-15 ReadCoor, LLC METHODS AND SYSTEMS FOR SAMPLE PROCESSING OR ANALYSIS
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
CN115362264A (zh) 2020-01-29 2022-11-18 10X基因组学有限公司 用于分析物检测的组合物和方法
US20220049303A1 (en) 2020-08-17 2022-02-17 Readcoor, Llc Methods and systems for spatial mapping of genetic variants

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5102785A (en) * 1987-09-28 1992-04-07 E. I. Du Pont De Nemours And Company Method of gene mapping
JPH02299598A (ja) * 1989-04-14 1990-12-11 Ro Inst For Molecular Genetics & Geneteic Res 微視的サイズの別個の粒子と結合している核酸試料中のごく短い配列の全部または一部のオリゴヌクレチドプローブとのハイブリダイゼーションによる決定法
US5650274A (en) * 1993-06-25 1997-07-22 Hitachi, Ltd. DNA analyzing method
GB9401200D0 (en) * 1994-01-21 1994-03-16 Medical Res Council Sequencing of nucleic acids
US5552278A (en) * 1994-04-04 1996-09-03 Spectragen, Inc. DNA sequencing by stepwise ligation and cleavage
US5604097A (en) * 1994-10-13 1997-02-18 Spectragen, Inc. Methods for sorting polynucleotides using oligonucleotide tags
EP0832287B1 (en) * 1995-06-07 2007-10-10 Solexa, Inc Oligonucleotide tags for sorting and identification

Also Published As

Publication number Publication date
CN1230226A (zh) 1999-09-29
JP2000515006A (ja) 2000-11-14
PL331513A1 (en) 1999-07-19
NO985698L (no) 1999-02-08
DE69737450D1 (de) 2007-04-19
EP0923650B1 (en) 2007-03-07
CN1195872C (zh) 2005-04-06
HUP0003944A2 (en) 2001-03-28
JP4124377B2 (ja) 2008-07-23
EP0923650A1 (en) 1999-06-23
AU733782B2 (en) 2001-05-24
AU3374097A (en) 1998-01-05
WO1997046704A1 (en) 1997-12-11
HUP0003944A3 (en) 2003-08-28
NO985698D0 (no) 1998-12-04
ATE356221T1 (de) 2007-03-15
HK1021206A1 (en) 2000-06-02
CA2256700A1 (en) 1997-12-11
DE69737450T2 (de) 2007-11-29

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