CZ289739B6 - Způsob identifikace E. coli DSM 6601 a sekvence pro pouľití při identifikaci - Google Patents
Způsob identifikace E. coli DSM 6601 a sekvence pro pouľití při identifikaci Download PDFInfo
- Publication number
- CZ289739B6 CZ289739B6 CZ20001873A CZ20001873A CZ289739B6 CZ 289739 B6 CZ289739 B6 CZ 289739B6 CZ 20001873 A CZ20001873 A CZ 20001873A CZ 20001873 A CZ20001873 A CZ 20001873A CZ 289739 B6 CZ289739 B6 CZ 289739B6
- Authority
- CZ
- Czechia
- Prior art keywords
- dna sequence
- nucleotide sequences
- following nucleotide
- escherichia coli
- dna
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000588724 Escherichia coli Species 0.000 title claims abstract description 16
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 14
- 238000003752 polymerase chain reaction Methods 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims description 6
- 241000020089 Atacta Species 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 abstract description 4
- 125000003729 nucleotide group Chemical group 0.000 abstract description 4
- 108020004414 DNA Proteins 0.000 description 8
- 239000000427 antigen Substances 0.000 description 6
- 230000001717 pathogenic effect Effects 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 102000036639 antigens Human genes 0.000 description 3
- 108091007433 antigens Proteins 0.000 description 3
- 230000000295 complement effect Effects 0.000 description 3
- 238000004925 denaturation Methods 0.000 description 3
- 230000036425 denaturation Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000007918 pathogenicity Effects 0.000 description 3
- 230000003321 amplification Effects 0.000 description 2
- 238000010367 cloning Methods 0.000 description 2
- 101150043770 fimA gene Proteins 0.000 description 2
- 101150077334 focA gene Proteins 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 101150014100 pilA gene Proteins 0.000 description 2
- 101150004519 pilC gene Proteins 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 108020000946 Bacterial DNA Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 108010040721 Flagellin Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 241000589500 Thermus aquaticus Species 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 238000003505 heat denaturation Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 239000001226 triphosphate Substances 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19751243 | 1997-11-19 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CZ20001873A3 CZ20001873A3 (cs) | 2000-10-11 |
| CZ289739B6 true CZ289739B6 (cs) | 2002-03-13 |
Family
ID=7849197
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CZ20001873A CZ289739B6 (cs) | 1997-11-19 | 1998-11-18 | Způsob identifikace E. coli DSM 6601 a sekvence pro pouľití při identifikaci |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US6489107B1 (enExample) |
| EP (1) | EP1038035A1 (enExample) |
| JP (1) | JP2004516801A (enExample) |
| CZ (1) | CZ289739B6 (enExample) |
| EE (1) | EE200000231A (enExample) |
| HU (1) | HUP0004409A3 (enExample) |
| NO (1) | NO20002550L (enExample) |
| PL (1) | PL190847B1 (enExample) |
| WO (1) | WO1999025870A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19915772C2 (de) * | 1998-11-18 | 2001-08-30 | Pharma Zentrale Gmbh | Verfahren zur Identifizierung von Escherichia coli Stamm DSM 6601 |
| US20050064447A1 (en) * | 2001-04-18 | 2005-03-24 | Sheng-He Huang | Probiotic therapy of neonatal meningitis and method of using E. coli virulence determinatns |
| DE10155928A1 (de) * | 2001-11-15 | 2003-06-12 | Degussa | recA-negativer und rhaB-negativer Mikroorganismus |
| EP3506917A4 (en) | 2016-08-31 | 2020-05-06 | President and Fellows of Harvard College | MANIPULATED BACTERIA PRODUCING THERAPEUTIC PROTEINS AND METHOD FOR USE THEREOF |
| CN109576198B (zh) * | 2018-11-09 | 2022-06-07 | 南京工业大学 | 一株敲除fimA基因的重组大肠杆菌及其构建方法与应用 |
| WO2022060848A1 (en) * | 2020-09-15 | 2022-03-24 | Northeastern University | Plasmid vectors for in vivo selection-free use with the probiotic e. coli nissle |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19713543B4 (de) * | 1997-04-02 | 2007-01-11 | Pharma-Zentrale Gmbh | Bakterielle Plasmide |
| DE19751242C2 (de) | 1997-11-19 | 2001-02-08 | Pharma Zentrale Gmbh | DNA-Sequenzen aus Fimbriengenen von Escherichia coli Stamm DSM 6601 |
-
1998
- 1998-11-18 WO PCT/EP1998/007398 patent/WO1999025870A1/de not_active Ceased
- 1998-11-18 HU HU0004409A patent/HUP0004409A3/hu not_active Application Discontinuation
- 1998-11-18 PL PL340330A patent/PL190847B1/pl unknown
- 1998-11-18 JP JP2000521233A patent/JP2004516801A/ja active Pending
- 1998-11-18 EP EP98966241A patent/EP1038035A1/de not_active Withdrawn
- 1998-11-18 EE EEP200000231A patent/EE200000231A/xx unknown
- 1998-11-18 CZ CZ20001873A patent/CZ289739B6/cs not_active IP Right Cessation
- 1998-11-18 US US09/554,724 patent/US6489107B1/en not_active Expired - Fee Related
-
2000
- 2000-05-18 NO NO20002550A patent/NO20002550L/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| NO20002550L (no) | 2000-07-18 |
| CZ20001873A3 (cs) | 2000-10-11 |
| JP2004516801A (ja) | 2004-06-10 |
| PL340330A1 (en) | 2001-01-29 |
| NO20002550D0 (no) | 2000-05-18 |
| US6489107B1 (en) | 2002-12-03 |
| EP1038035A1 (de) | 2000-09-27 |
| EE200000231A (et) | 2001-06-15 |
| HUP0004409A3 (en) | 2001-09-28 |
| HUP0004409A2 (hu) | 2001-04-28 |
| PL190847B1 (pl) | 2006-02-28 |
| WO1999025870A1 (de) | 1999-05-27 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PD00 | Pending as of 2000-06-30 in czech republic | ||
| MM4A | Patent lapsed due to non-payment of fee |
Effective date: 20061118 |