CY1111959T1 - Μεθοδος για την βελτιωση της διαπερατοτητας των κυτταρων σε ξενα σωματιδια - Google Patents

Μεθοδος για την βελτιωση της διαπερατοτητας των κυτταρων σε ξενα σωματιδια

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Publication number
CY1111959T1
CY1111959T1 CY20111101010T CY111101010T CY1111959T1 CY 1111959 T1 CY1111959 T1 CY 1111959T1 CY 20111101010 T CY20111101010 T CY 20111101010T CY 111101010 T CY111101010 T CY 111101010T CY 1111959 T1 CY1111959 T1 CY 1111959T1
Authority
CY
Cyprus
Prior art keywords
cell
pathogen
foreign particles
membrane
improving cell
Prior art date
Application number
CY20111101010T
Other languages
English (en)
Inventor
Jyotsana S Shah
Helena Weltman
Original Assignee
Id-Fish Technology, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Id-Fish Technology, Inc. filed Critical Id-Fish Technology, Inc.
Publication of CY1111959T1 publication Critical patent/CY1111959T1/el

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
    • A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
    • A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5306—Improving reaction conditions, e.g. reduction of non-specific binding, promotion of specific binding
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G01N33/567—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds utilising isolate of tissue or organ as binding agent
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G01N33/5695—Mycobacteria

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Inorganic Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Η παρούσα εφεύρεση παρέχει μια μέθοδο για να επιτραπεί στα ξένα σωματίδια να διεισδύσουν, πολύ αποτελεσματικώς, στο τοίχωμα των κυττάρων, στην μεμβράνη των κυττάρων, στην μεμβράνη των οργανιδίων και/ή στην πυρηνική μεμβράνη ενός κυττάρου και για να υβριδοποιήσουν ή δεσμεύσουν τον συμπληρωματικό στόχο στο κύτταρο. Τα κύτταρα μπορεί να είναι από μια καλλιέργεια ή από βιολογικά δείγματα που λαμβάνονται από έναν ασθενή. Το ξένο σωματίδιο μπορεί να είναι ένας ιχνηθέτης συνιστάμενος, για παράδειγμα, είτε μεμονωμένα ή στον οποιονδήποτε συνδυασμό από δύο ή περισσότερα εκ των ακόλουθων: DNA, RNA, πυρηνικά οξέα πεπτιδίων (ΡΝΑ), γλυκοπεπτίδια, λιποπεπτίδια, γλυκολιπίδια ή πριόνια. Ο στόχος είναι ένα κύτταρο, ένα συστατικό κυττάρου ή, κατά προτίμηση, ένα παθογόνο ή συστατικό παθογόνου. Το παθογόνο μπορεί, για παράδειγμα, να είναι βακτήρια, μύκητες, ζυμομύκητας ή ιοί.
CY20111101010T 2005-07-28 2011-10-25 Μεθοδος για την βελτιωση της διαπερατοτητας των κυτταρων σε ξενα σωματιδια CY1111959T1 (el)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US70332905P 2005-07-28 2005-07-28
EP06788834A EP1924252B1 (en) 2005-07-28 2006-07-27 Method for improving cell permeability to foreign particles

Publications (1)

Publication Number Publication Date
CY1111959T1 true CY1111959T1 (el) 2015-11-04

Family

ID=37709226

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20111101010T CY1111959T1 (el) 2005-07-28 2011-10-25 Μεθοδος για την βελτιωση της διαπερατοτητας των κυτταρων σε ξενα σωματιδια

Country Status (16)

Country Link
US (1) US8632963B2 (el)
EP (1) EP1924252B1 (el)
JP (1) JP5132557B2 (el)
CN (1) CN101272773A (el)
AT (1) ATE523193T1 (el)
AU (1) AU2006275632B2 (el)
BR (1) BRPI0615958B8 (el)
CA (1) CA2641599C (el)
CY (1) CY1111959T1 (el)
DK (1) DK1924252T3 (el)
ES (1) ES2372538T3 (el)
PL (1) PL1924252T3 (el)
PT (1) PT1924252E (el)
SI (1) SI1924252T1 (el)
WO (1) WO2007016379A2 (el)
ZA (1) ZA200801859B (el)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
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US8758990B2 (en) * 2005-07-28 2014-06-24 Id-Fish Technology, Inc. Methods for detecting and differentiating Mycobacterium genus and Mycobacterium avium complex in a sample or culture
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
WO2008066871A2 (en) 2006-11-30 2008-06-05 Id-Fish Technology Inc. Nucleic acid probes and methods for detecting plasmodium parasites
US8323901B2 (en) * 2006-11-30 2012-12-04 Id-Fish Technology, Inc. Nucleic acid probes and methods for detecting plasmodium parasites
JP5241100B2 (ja) * 2006-12-28 2013-07-17 シスメックス株式会社 抗原賦活化液、抗原賦活化方法及び細胞の検出方法
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
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CA2861667C (en) * 2007-10-01 2017-06-13 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system and methods of use
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
JP5645840B2 (ja) 2008-12-02 2014-12-24 株式会社Ｗａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｊａｐａｎ リン原子修飾核酸の合成方法
CN102596204B (zh) 2009-07-06 2016-11-23 波涛生命科学有限公司 新的核酸前药及其使用方法
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JP6128529B2 (ja) 2011-07-19 2017-05-17 ウェイブ ライフ サイエンシズ リミテッドＷａｖｅ Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ Ｌｔｄ． 官能化核酸の合成のための方法
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PL2872485T3 (pl) 2012-07-13 2021-05-31 Wave Life Sciences Ltd. Asymetryczna grupa pomocnicza
AU2013287630B2 (en) 2012-07-13 2017-05-25 Shin Nippon Biomedical Laboratories, Ltd. Chiral nucleic acid adjuvant
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Also Published As

Publication number Publication date
JP2009502177A (ja) 2009-01-29
ES2372538T3 (es) 2012-01-23
CA2641599A1 (en) 2007-02-08
US8632963B2 (en) 2014-01-21
JP5132557B2 (ja) 2013-01-30
BRPI0615958B8 (pt) 2021-05-25
US20070042358A1 (en) 2007-02-22
BRPI0615958B1 (pt) 2020-11-10
PT1924252E (pt) 2011-12-16
WO2007016379A3 (en) 2007-07-12
DK1924252T3 (da) 2011-11-14
CN101272773A (zh) 2008-09-24
ATE523193T1 (de) 2011-09-15
BRPI0615958A2 (pt) 2011-05-31
AU2006275632B2 (en) 2011-11-03
EP1924252B1 (en) 2011-09-07
ZA200801859B (en) 2009-07-29
WO2007016379A2 (en) 2007-02-08
AU2006275632A1 (en) 2007-02-08
PL1924252T3 (pl) 2012-02-29
EP1924252A2 (en) 2008-05-28
EP1924252A4 (en) 2010-03-17
SI1924252T1 (sl) 2011-12-30
CA2641599C (en) 2012-07-10

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