CY1107083T1 - Συνδετηρες για ρυθμιση της εκφρασης εξωγενων γονιδιων μεσω συμπλοκου υποδοχεα εκδυζονης - Google Patents

Συνδετηρες για ρυθμιση της εκφρασης εξωγενων γονιδιων μεσω συμπλοκου υποδοχεα εκδυζονης

Info

Publication number
CY1107083T1
CY1107083T1 CY20071101577T CY071101577T CY1107083T1 CY 1107083 T1 CY1107083 T1 CY 1107083T1 CY 20071101577 T CY20071101577 T CY 20071101577T CY 071101577 T CY071101577 T CY 071101577T CY 1107083 T1 CY1107083 T1 CY 1107083T1
Authority
CY
Cyprus
Prior art keywords
gene expression
receptors
connectors
response element
ligand
Prior art date
Application number
CY20071101577T
Other languages
English (en)
Inventor
Glenn Richard Carlson
Dean Ervin Cress
Tarlochan Singh Dhadialla
Robert Eugene Hormann
Dat Phat Le
Original Assignee
Intrexon Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intrexon Corporation filed Critical Intrexon Corporation
Publication of CY1107083T1 publication Critical patent/CY1107083T1/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D319/00—Heterocyclic compounds containing six-membered rings having two oxygen atoms as the only ring hetero atoms
    • C07D319/10—1,4-Dioxanes; Hydrogenated 1,4-dioxanes
    • C07D319/14—1,4-Dioxanes; Hydrogenated 1,4-dioxanes condensed with carbocyclic rings or ring systems
    • C07D319/16—1,4-Dioxanes; Hydrogenated 1,4-dioxanes condensed with carbocyclic rings or ring systems condensed with one six-membered ring
    • C07D319/18—Ethylenedioxybenzenes, not substituted on the hetero ring
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C243/00—Compounds containing chains of nitrogen atoms singly-bound to each other, e.g. hydrazines, triazanes
    • C07C243/24—Hydrazines having nitrogen atoms of hydrazine groups acylated by carboxylic acids
    • C07C243/38—Hydrazines having nitrogen atoms of hydrazine groups acylated by carboxylic acids with acylating carboxyl groups bound to carbon atoms of six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Peptides Or Proteins (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

Η εφεύρεση αυτή αφορά μία βελτιωμένη μέθοδο για ρύθμιση έκφρασης εξωγενούς γονιδίου όπου ένα σύμπλοκο υποδοχέα εκδυζόνης που περιλαμβάνει : ένα πεδίο σύνδεσης DNA, πεδίο σύνδεσης συνδετήρα, πεδίο μετενεργοποίησης και ένας συνδετήρας φέρεται σε επαφή με ένα DNA κατασκεύασμα που περιλαμβάνει : το εξωγενές γονίδιο και ένα στοιχείο απόκρισης όπου το εξωγενές γονίδιο είναι κάτω από τον έλεγχο του στοιχείου απόκρισης και σύνδεση του πεδίου σύνδεσης DNA προς το στοιχείο απόκρισης παρουσία του συνδετήρα έχει σαν αποτέλεσμα ενεργοποίηση ή καταστολή του γονιδίου. Η βελτίωση βασίζεται σε μία ομάδα επιλογής μη-στεροειδών συνδετήρων που δείχνουν βελτιωμένη δραστικότητα έναντι γνωστών συνδετήρων.
CY20071101577T 1998-06-17 2007-12-12 Συνδετηρες για ρυθμιση της εκφρασης εξωγενων γονιδιων μεσω συμπλοκου υποδοχεα εκδυζονης CY1107083T1 (el)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US8954698P 1998-06-17 1998-06-17
US09/315,451 US6258603B1 (en) 1998-06-17 1999-05-02 Ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
EP99304444A EP0965644B1 (en) 1998-06-17 1999-06-08 Ligands for modulating the expression of exogenous genes via an ecdysone receptor complex

Publications (1)

Publication Number Publication Date
CY1107083T1 true CY1107083T1 (el) 2012-10-24

Family

ID=26780689

Family Applications (1)

Application Number Title Priority Date Filing Date
CY20071101577T CY1107083T1 (el) 1998-06-17 2007-12-12 Συνδετηρες για ρυθμιση της εκφρασης εξωγενων γονιδιων μεσω συμπλοκου υποδοχεα εκδυζονης

Country Status (14)

Country Link
US (2) US6258603B1 (el)
EP (1) EP0965644B1 (el)
JP (1) JP4537512B2 (el)
KR (1) KR100563143B1 (el)
CN (1) CN1296480C (el)
AT (1) ATE378414T1 (el)
AU (1) AU765306B2 (el)
BR (1) BR9902283A (el)
CY (1) CY1107083T1 (el)
DE (1) DE69937519T2 (el)
DK (1) DK0965644T3 (el)
ES (1) ES2296370T3 (el)
PT (1) PT965644E (el)
TW (1) TWI224109B (el)

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6875727B2 (en) * 1997-12-23 2005-04-05 Syngenta Crop Protection, Inc. Use of macrolides in pest control
AUPP135698A0 (en) 1998-01-15 1998-02-05 Commonwealth Scientific And Industrial Research Organisation Insectical modalities
AU2001241682A1 (en) * 2000-02-24 2001-09-03 The Salk Institute For Biological Studies Gene expression system based on chimeric receptors
US20040033600A1 (en) 2001-03-21 2004-02-19 Palli Subba Reddy Ecdysone receptor-based inducible gene expression system
ATE338135T1 (de) 2000-03-22 2006-09-15 Rheogene Holdings Inc Induziertes genexpressionssystem basierend auf ecdysonrezeptoren
US8105825B2 (en) * 2000-10-03 2012-01-31 Intrexon Corporation Multiple inducible gene regulation system
US6576422B1 (en) 2000-10-17 2003-06-10 Rohm And Haas Company Method for identifying products employing gene expression
WO2002066615A2 (en) 2001-02-20 2002-08-29 Rheogene, Inc. Novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
MXPA03007493A (es) * 2001-02-20 2004-10-15 Rheogene Holdings Inc Nuevo sistema de expresion genetica inducible a base de receptor de ecdisona/receptor x retinoide de invertebrado.
US8715959B2 (en) * 2001-02-20 2014-05-06 Intrexon Corporation Substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system
ES2392508T3 (es) * 2001-02-20 2012-12-11 Intrexon Corporation Receptores X retinoides quiméricos y su uso en un sistema inducible de expresión génica basado en receptores de ecdisona novedoso
MXPA04002810A (es) 2001-09-26 2005-06-06 Rheogene Holdings Inc Acidos nucleicos receptores de ecdisona de saltarilla, polipeptidos, y sus usos.
GB2387599B (en) * 2002-04-17 2005-08-10 Jason Peter Brown Methods for producing antibodies
US20050228016A1 (en) * 2002-06-13 2005-10-13 Enrique Michelotti Tetrahydroquinolines for modulating the expression of exogenous genes via an ecdysone receptor complex
US20040082618A1 (en) * 2002-07-03 2004-04-29 Rajneesh Taneja Liquid dosage forms of acid labile drugs
US7375093B2 (en) * 2002-07-05 2008-05-20 Intrexon Corporation Ketone ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
AU2003284316A1 (en) * 2002-10-24 2004-05-13 Albert Einstein College Of Medicine Of Yeshiva University Caged ligands and uses thereof
AU2011202682B2 (en) * 2003-02-10 2013-11-28 Intrexon Corporation Diacylhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex
US7304161B2 (en) * 2003-02-10 2007-12-04 Intrexon Corporation Diaclhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex
US7304162B2 (en) * 2003-02-21 2007-12-04 Intrexon Corporation Oxadiazoline ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US7456315B2 (en) 2003-02-28 2008-11-25 Intrexon Corporation Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
US20050164210A1 (en) * 2004-01-23 2005-07-28 Vivek Mittal Regulated polymerase III expression systems and related methods
US7935510B2 (en) 2004-04-30 2011-05-03 Intrexon Corporation Mutant receptors and their use in a nuclear receptor-based inducible gene expression system
US8115059B1 (en) 2005-07-22 2012-02-14 University Of Kentucky Research Foundation Gene expression modulation system for use in plants and method for modulating gene expression in plants
CA2689137C (en) 2007-05-29 2017-05-02 Intrexon Corporation Chiral diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
JP2010536373A (ja) 2007-08-23 2010-12-02 イントレキソン コーポレーション 疾患を診断するための方法および組成物
BRPI0817009A2 (pt) * 2007-09-17 2014-10-07 Rohm & Haas Sistema de expressão de gene para a expressão controlável de resposta ao etileno em uma célula de planta, composição, e, métodos para produzir uma planta transgênica, e para modular sensibilidade ao etileno em uma planta.
CA2715080C (en) 2007-09-28 2021-09-28 Intrexon Corporation Therapeutic gene-switch constructs and bioreactors for the expression of biotherapeutic molecules, and uses thereof
CA2702058A1 (en) 2007-10-08 2009-04-16 Intrexon Corporation Engineered dendritic cells and uses for the treatment of cancer
BRPI0908747A2 (pt) 2008-03-14 2023-04-18 Intrexon Corp Ligantes esteróides e seu uso em modulação de comutador genético
JP2012519486A (ja) 2009-03-05 2012-08-30 ローム アンド ハース カンパニー 植物におけるエチレン感受性を調節する鍵となるエチレンホルモンシグナル伝達経路タンパク質の標的ターンオーバーの制御
WO2011119773A1 (en) 2010-03-23 2011-09-29 Roeth Jeremiah F Vectors conditionally expressing therapeutic proteins, host cells comprising the vectors, and uses thereof
CN103534355A (zh) 2011-03-04 2014-01-22 英特瑞克斯顿股份有限公司 条件性表达蛋白质的载体
WO2012145572A1 (en) 2011-04-20 2012-10-26 The Trustees Of The University Of Pennsylvania Regimens and compositions for aav-mediated passive immunization of airborne pathogens
AU2014227571B2 (en) 2013-03-15 2017-02-02 Intrexon Corporation Boron-containing diacylhydrazines
WO2015012924A2 (en) 2013-04-29 2015-01-29 The Trustees Of The University Of Pennsylvania Tissue preferential codon modified expression cassettes, vectors containing same, and use thereof
EP3194359B1 (en) 2014-09-17 2020-04-08 Intrexon Corporation Boron-containing diacylhydrazine compounds
WO2016200543A2 (en) 2015-05-13 2016-12-15 The Trustees Of The University Of Pennsylvania Aav-mediated expression of anti-inluenza antibodies and methods of use thereof
UY37343A (es) 2016-07-25 2018-02-28 Intrexon Corp Control del fenotipo en plantas
WO2018089527A1 (en) 2016-11-09 2018-05-17 Intrexon Corporation Frataxin expression constructs
AU2018229293A1 (en) 2017-02-28 2019-08-29 Janssen Biotech, Inc. Influenza vaccines based on AAV vectors
LT3589730T (lt) 2017-02-28 2024-03-12 The Trustees Of The University Of Pennsylvania Adenoasocijuoto viruso (aav) monofiletinės grupės f vektorius, ir jo panaudojimo būdai
JOP20190200A1 (ar) 2017-02-28 2019-08-27 Univ Pennsylvania تركيبات نافعة في معالجة ضمور العضل النخاعي
TW202237850A (zh) 2020-12-01 2022-10-01 賓州大學委員會 具有組織特異性靶向基序的新穎構成物及含有其之組成物
US20240207452A1 (en) 2021-04-23 2024-06-27 The Trustees Of The University Of Pennsylvania Novel compositions with brain-specific targeting motifs and compositions containing same
EP4409010A1 (en) 2021-10-02 2024-08-07 The Trustees of The University of Pennsylvania Novel aav capsids and compositions containing same
WO2023147304A1 (en) 2022-01-25 2023-08-03 The Trustees Of The University Of Pennsylvania Aav capsids for improved heart transduction and detargeting of liver
WO2024130070A2 (en) 2022-12-17 2024-06-20 The Trustees Of The University Of Pennsylvania Recombinant aav capsids with cardiac- and skeletal muscle- specific targeting motifs and uses thereof
WO2024130067A2 (en) 2022-12-17 2024-06-20 The Trustees Of The University Of Pennsylvania Recombinant aav mutant vectors with cardiac and skeletal muscle-specific targeting motifs and compositions containing same

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5117057A (en) * 1985-10-21 1992-05-26 Rohm And Haas Company Insecticidal N' substituted-N-N'-disubstituted-hydrazines
US5530028A (en) * 1992-11-23 1996-06-25 Rohm And Haas Company Insecticidal N'-substituted-N,N'-diacylhydrazines
US5344958A (en) * 1992-11-23 1994-09-06 Rohm And Haas Company Insecticidal N'-substituted-N,N'-diacylhydrazines
JP4004555B2 (ja) * 1993-09-29 2007-11-07 ダウ アグロサイエンシィズ エルエルシー 殺虫化合物
US5482962A (en) * 1994-07-22 1996-01-09 Rohm And Haas Company Insecticidal N,N'-disubstituted-N-N'-diacylhydrazines
CA2213340C (en) * 1995-03-03 2011-07-26 Novartis Ag Control of gene expression in plants by receptor mediated transactivation in the presence of a chemical ligand
WO1996037609A1 (en) * 1995-05-26 1996-11-28 Zeneca Limited A gene switch comprising an ecdysone receptor
IL126418A0 (en) * 1996-04-05 1999-05-09 Salk Inst For Biological Studi Hormone-mediated methods for modulating expression of exogenous genes and pharmaceutical compositions for modulating the same
US6333318B1 (en) * 1998-05-14 2001-12-25 The Salk Institute For Biological Studies Formulations useful for modulating expression of exogenous genes in mammalian systems, and products related thereto
AR021484A1 (es) * 1998-09-10 2002-07-24 Pioneer Hi Bred Int Nuevos receptores de ecdisona y metodos de uso de los mismos

Also Published As

Publication number Publication date
ATE378414T1 (de) 2007-11-15
KR100563143B1 (ko) 2006-03-22
BR9902283A (pt) 2000-05-02
DK0965644T3 (da) 2008-01-28
ES2296370T3 (es) 2008-04-16
EP0965644A3 (en) 2004-01-07
US6258603B1 (en) 2001-07-10
KR20000006256A (ko) 2000-01-25
CN1296480C (zh) 2007-01-24
EP0965644A2 (en) 1999-12-22
AU3390099A (en) 2000-02-10
EP0965644B1 (en) 2007-11-14
JP2000037189A (ja) 2000-02-08
TWI224109B (en) 2004-11-21
JP4537512B2 (ja) 2010-09-01
DE69937519D1 (de) 2007-12-27
PT965644E (pt) 2007-12-03
US20010044151A1 (en) 2001-11-22
DE69937519T2 (de) 2008-09-04
CN1251389A (zh) 2000-04-26
AU765306B2 (en) 2003-09-18

Similar Documents

Publication Publication Date Title
CY1107083T1 (el) Συνδετηρες για ρυθμιση της εκφρασης εξωγενων γονιδιων μεσω συμπλοκου υποδοχεα εκδυζονης
WO2004078924A3 (en) Bioavailable diacylhydrazine ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
WO2005017126A3 (en) Oxadiazoline ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
WO2004072254A3 (en) Diacylhydrazine ligands for modulating the expression of exogenous genes in mammalian systems via an ecdysone receptor complex
WO2004005478A3 (en) Ketone ligands for modulating the expression of exogenous genes via an ecdysone receptor complex
DE59810783D1 (de) Hub- und Tiefensteuersystem für Anbaugeräte
DE59703664D1 (de) Mikrokapseln unter Verwendung von Iminooxadiazindion-Polyisocyanaten
NO20011962D0 (no) Dimere trombopoetinpeptidetterligninger som binder til MP1- reseptor og som har trombopoetinaktivitet
DE19882125T1 (de) Direkte Abscheidung von Palladium
ATE293699T1 (de) Kontrolle der pflanzengenexpression durch rezeptor-vermittelte transaktivation in gegenwart von einem chemischen ligand
DE69623970D1 (de) Formulierung von d-threo methylphenidat mit verzögerter freisetzung
DE19680256T1 (de) Kleine Inhibitor-Moleküle der Enzymaktivität von Rotamase
ATE271532T1 (de) Hydroformylierung von olefinen unter verwendung von bis(phosphor) trägerliganden
DE69713725D1 (de) Regelung des Oberflächenpotentials von isolierenden Proben während einer Oberflächenanalyse
TR200100389T2 (tr) Glomerulpatinin tedavisi veya önlenmesi için terkip.
WO1999019513A3 (en) Methods for detecting compounds which modulate the activity of an lpa receptor
DE69820441T2 (de) Verringerung von unerwünschten nebenwirkungen während des gebrauchs von absorbierenden artikeln durch ph-kontrolle
DE19581385T1 (de) Automatische Tiefenkontrolle für Grabenzieher
ATE371041T1 (de) Kontrolle der immunantworten durch regulierung der aktivität von glykosyltransferasen
FI972754A (fi) Uusi monoklonaalinen vasta-aine, jolla on tyypin II fosfolipaasia A2 inhiboiva aktiivisuus, ja tämän osan sisältävä proteiini
DE69625999D1 (de) System für ausführungssteuerung von sequentiellen programmen
NO986058D0 (no) Ikke-lineµre redusert-fasefiltere for aktiv st÷ykontroll
DE69818773D1 (de) Kommunikationsvorrichtung, Kommunikationsverfahren und Aufzeichnungsmedium mit dem Rechnerprogramm zur Durchführung des Verfahrens
DE69317120D1 (de) Verfahren zur kontrolle von destillativen katalytischen veretherungen
WO2000008181A3 (en) Chp polypeptide, a ligand of pak65