CS257291A3 - Multi-layered, non-sequential discontinuous polyacrylamide gels, forming equilibrium molecular sieves for biomolecules separation - Google Patents
Multi-layered, non-sequential discontinuous polyacrylamide gels, forming equilibrium molecular sieves for biomolecules separationInfo
- Publication number
- CS257291A3 CS257291A3 CS912572A CS257291A CS257291A3 CS 257291 A3 CS257291 A3 CS 257291A3 CS 912572 A CS912572 A CS 912572A CS 257291 A CS257291 A CS 257291A CS 257291 A3 CS257291 A3 CS 257291A3
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- pct
- molecular sieves
- separation
- biomolecules
- layered
- Prior art date
Links
- 239000000499 gel Substances 0.000 title abstract 3
- 239000002808 molecular sieve Substances 0.000 title abstract 2
- 238000000926 separation method Methods 0.000 title abstract 2
- URGAHOPLAPQHLN-UHFFFAOYSA-N sodium aluminosilicate Chemical compound [Na+].[Al+3].[O-][Si]([O-])=O.[O-][Si]([O-])=O URGAHOPLAPQHLN-UHFFFAOYSA-N 0.000 title abstract 2
- 229920002401 polyacrylamide Polymers 0.000 title 1
- 239000000126 substance Substances 0.000 abstract 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N acrylic acid group Chemical group C(C=C)(=O)O NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 238000012512 characterization method Methods 0.000 abstract 1
- 239000002131 composite material Substances 0.000 abstract 1
- 229920002521 macromolecule Polymers 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 238000005192 partition Methods 0.000 abstract 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44773—Multi-stage electrophoresis, e.g. two-dimensional electrophoresis
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Environmental & Geological Engineering (AREA)
- Engineering & Computer Science (AREA)
- Dispersion Chemistry (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Processing And Handling Of Plastics And Other Materials For Molding In General (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Processes Of Treating Macromolecular Substances (AREA)
- Absorbent Articles And Supports Therefor (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US62705490A | 1990-12-13 | 1990-12-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS257291A3 true CS257291A3 (en) | 1992-06-17 |
Family
ID=24512990
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS912572A CS257291A3 (en) | 1990-12-13 | 1991-08-20 | Multi-layered, non-sequential discontinuous polyacrylamide gels, forming equilibrium molecular sieves for biomolecules separation |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US5387325A (cs) |
| EP (1) | EP0563259B1 (cs) |
| AT (1) | ATE184211T1 (cs) |
| AU (1) | AU9162191A (cs) |
| CA (1) | CA2098306A1 (cs) |
| CS (1) | CS257291A3 (cs) |
| DE (1) | DE69131600D1 (cs) |
| WO (1) | WO1992010277A1 (cs) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991017985A1 (en) * | 1990-05-23 | 1991-11-28 | Lipogenics, Inc. | Processes for recovering tocotrienols, tocopherols and tocotrienol-like compounds |
| US5083315A (en) * | 1990-12-13 | 1992-01-28 | Johnson & Johnson Medical, Inc. | Unisex scrub shirt and methods for making same |
| US5589104A (en) * | 1993-12-30 | 1996-12-31 | Bambeck; Gregory S. | Electrophoresis separation gel and a method for preparing an electrophoresis separation gel |
| US5925229A (en) * | 1996-05-03 | 1999-07-20 | The Regents Of The University Of California | Low density lipoprotein fraction assay for cardiac disease risk |
| US7214477B1 (en) | 1999-07-26 | 2007-05-08 | The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services | Layered device with capture regions for cellular analysis |
| US6969615B2 (en) * | 1999-07-26 | 2005-11-29 | 20/20 Genesystems, Inc. | Methods, devices, arrays and kits for detecting and analyzing biomolecules |
| US7838222B2 (en) * | 1999-07-26 | 2010-11-23 | United States of America/ NIH | Methods, devices and kits for multiplex blotting of biological samples from multi-well plates |
| ES2269171T3 (es) | 1999-07-26 | 2007-04-01 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Heal | Dispositivo en capas con regiones de captura para analisis celular. |
| US6379519B1 (en) * | 1999-09-01 | 2002-04-30 | Mirador Dna Design Inc. | Disposable thermoformed electrophoresis cassette |
| US20060147926A1 (en) * | 2002-11-25 | 2006-07-06 | Emmert-Buck Michael R | Method and apparatus for performing multiple simultaneous manipulations of biomolecules in a two-dimensional array |
| CN105137070B (zh) * | 2015-08-03 | 2018-07-24 | 复旦大学附属中山医院 | 改良凝胶电泳检测血清前β1高密度脂蛋白的方法和应用 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3384564A (en) * | 1962-11-21 | 1968-05-21 | Mount Sinai Hospital Res Found | Electrophoretic process for simultaneously spearating and concentrating particles |
| US4189370A (en) * | 1978-06-21 | 1980-02-19 | Societe Sebia | Process for obtaining gels of N-methylol-acrylamide copolymers and application of said gels for the stepped gradient separation of seric lipoproteins |
| US4312727A (en) * | 1979-03-23 | 1982-01-26 | The Cleveland Clinic Foundation | Glyoxal agarose and zonal immobilization of proteins therewith |
| US4319975A (en) * | 1980-10-20 | 1982-03-16 | Fmc Corporation | Derivatized agarose and method of making and using same |
| US4306956A (en) * | 1980-11-10 | 1981-12-22 | Miles Laboratories, Inc. | Discontinuous gel electrophoresis process |
| JPS59212751A (ja) * | 1983-05-19 | 1984-12-01 | Fuji Photo Film Co Ltd | 電気泳動媒体材料の製造方法 |
| JPS62173858A (ja) * | 1986-01-27 | 1987-07-30 | Nec Home Electronics Ltd | リモコン付テレビジヨン受像機の電源回路 |
| JPH0797339B2 (ja) * | 1987-04-21 | 1995-10-18 | 日本電気株式会社 | 制御装置システム |
| JPH01147355A (ja) * | 1987-12-02 | 1989-06-09 | Shimadzu Corp | 分子篩ユニット |
| JPH01173858A (ja) * | 1987-12-28 | 1989-07-10 | Fuji Photo Film Co Ltd | 電気泳動用媒体膜及び電気泳動方法 |
| JPH01263548A (ja) * | 1988-04-14 | 1989-10-20 | Fuji Photo Film Co Ltd | 電気泳動用媒体 |
| JP2597145B2 (ja) * | 1988-05-31 | 1997-04-02 | ハイモ株式会社 | 電気泳動用ゲルの製造方法 |
| US5078853A (en) * | 1991-03-22 | 1992-01-07 | Assay Technology, Inc. | Segmented electrophoretic separation system useful for lipid profiling |
-
1991
- 1991-08-20 CS CS912572A patent/CS257291A3/cs unknown
- 1991-12-13 EP EP92903113A patent/EP0563259B1/en not_active Expired - Lifetime
- 1991-12-13 DE DE69131600T patent/DE69131600D1/de not_active Expired - Lifetime
- 1991-12-13 US US08/074,847 patent/US5387325A/en not_active Expired - Fee Related
- 1991-12-13 CA CA002098306A patent/CA2098306A1/en not_active Abandoned
- 1991-12-13 AT AT92903113T patent/ATE184211T1/de not_active IP Right Cessation
- 1991-12-13 AU AU91621/91A patent/AU9162191A/en not_active Abandoned
- 1991-12-13 WO PCT/US1991/009348 patent/WO1992010277A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU9162191A (en) | 1992-07-08 |
| CA2098306A1 (en) | 1992-06-14 |
| WO1992010277A1 (en) | 1992-06-25 |
| EP0563259A1 (en) | 1993-10-06 |
| EP0563259A4 (cs) | 1994-01-12 |
| US5387325A (en) | 1995-02-07 |
| DE69131600D1 (de) | 1999-10-14 |
| EP0563259B1 (en) | 1999-09-08 |
| ATE184211T1 (de) | 1999-09-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PD00 | Pending as of 2000-06-30 in czech republic |