CS249368B1 - Method of pearls detran materials production for gel chromatography - Google Patents
Method of pearls detran materials production for gel chromatography Download PDFInfo
- Publication number
- CS249368B1 CS249368B1 CS8410609A CS1060984A CS249368B1 CS 249368 B1 CS249368 B1 CS 249368B1 CS 8410609 A CS8410609 A CS 8410609A CS 1060984 A CS1060984 A CS 1060984A CS 249368 B1 CS249368 B1 CS 249368B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- dextran
- gel
- solution
- water retention
- suspension
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 34
- 239000000463 material Substances 0.000 title claims description 11
- 238000005227 gel permeation chromatography Methods 0.000 title claims description 5
- 238000004519 manufacturing process Methods 0.000 title claims 2
- 239000011049 pearl Substances 0.000 title 1
- 229920002307 Dextran Polymers 0.000 claims description 46
- 239000000243 solution Substances 0.000 claims description 29
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 24
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 claims description 24
- 230000008569 process Effects 0.000 claims description 23
- 206010016807 Fluid retention Diseases 0.000 claims description 22
- 239000000725 suspension Substances 0.000 claims description 15
- BRLQWZUYTZBJKN-UHFFFAOYSA-N Epichlorohydrin Chemical compound ClCC1CO1 BRLQWZUYTZBJKN-UHFFFAOYSA-N 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 claims description 12
- 229910000033 sodium borohydride Inorganic materials 0.000 claims description 11
- 239000012279 sodium borohydride Substances 0.000 claims description 11
- 230000009467 reduction Effects 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 238000004132 cross linking Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 6
- 229920005654 Sephadex Polymers 0.000 claims description 5
- 239000011324 bead Substances 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 4
- 239000003381 stabilizer Substances 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000012670 alkaline solution Substances 0.000 claims description 2
- 239000006185 dispersion Substances 0.000 claims description 2
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000005987 sulfurization reaction Methods 0.000 claims description 2
- 239000004094 surface-active agent Substances 0.000 claims description 2
- KWXICGTUELOLSQ-UHFFFAOYSA-N 4-dodecylbenzenesulfonic acid Chemical compound CCCCCCCCCCCCC1=CC=C(S(O)(=O)=O)C=C1 KWXICGTUELOLSQ-UHFFFAOYSA-N 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 239000000499 gel Substances 0.000 description 15
- 239000002245 particle Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 238000007873 sieving Methods 0.000 description 4
- 239000004971 Cross linker Substances 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 150000002596 lactones Chemical class 0.000 description 3
- 229920002689 polyvinyl acetate Polymers 0.000 description 3
- 239000011118 polyvinyl acetate Substances 0.000 description 3
- 239000002002 slurry Substances 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 2
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 2
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 2
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 2
- 239000005642 Oleic acid Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002612 dispersion medium Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 230000029142 excretion Effects 0.000 description 2
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 2
- 239000002480 mineral oil Substances 0.000 description 2
- 235000010446 mineral oil Nutrition 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000004448 titration Methods 0.000 description 2
- SCYULBFZEHDVBN-UHFFFAOYSA-N 1,1-Dichloroethane Chemical compound CC(Cl)Cl SCYULBFZEHDVBN-UHFFFAOYSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- XTXRWKRVRITETP-UHFFFAOYSA-N Vinyl acetate Chemical compound CC(=O)OC=C XTXRWKRVRITETP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000013375 chromatographic separation Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 238000010908 decantation Methods 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 125000003976 glyceryl group Chemical group [H]C([*])([H])C(O[H])([H])C(O[H])([H])[H] 0.000 description 1
- 125000003055 glycidyl group Chemical group C(C1CO1)* 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-M hydroxide Chemical compound [OH-] XLYOFNOQVPJJNP-UHFFFAOYSA-M 0.000 description 1
- 125000003010 ionic group Chemical group 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000012312 sodium hydride Substances 0.000 description 1
- 229910000104 sodium hydride Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000012258 stirred mixture Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 238000005496 tempering Methods 0.000 description 1
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/291—Gel sorbents
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/22—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0009—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Glucans, e.g. polydextrose, alternan, glycogen; (alpha-1,4)(alpha-1,6)-D-Glucans; (alpha-1,3)(alpha-1,4)-D-Glucans, e.g. isolichenan or nigeran; (alpha-1,4)-D-Glucans; (alpha-1,3)-D-Glucans, e.g. pseudonigeran; Derivatives thereof
- C08B37/0021—Dextran, i.e. (alpha-1,4)-D-glucan; Derivatives thereof, e.g. Sephadex, i.e. crosslinked dextran
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2220/00—Aspects relating to sorbent materials
- B01J2220/50—Aspects relating to the use of sorbent or filter aid materials
- B01J2220/54—Sorbents specially adapted for analytical or investigative chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Dispersion Chemistry (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Compositions Of Macromolecular Compounds (AREA)
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS8410609A CS249368B1 (en) | 1984-12-29 | 1984-12-29 | Method of pearls detran materials production for gel chromatography |
| DD85282879A DD261297A3 (de) | 1984-12-29 | 1985-11-13 | Verfahren zur herstellung von dextranperlmaterialien fuer die gelchromatographie |
| DE8585308833T DE3568336D1 (en) | 1984-12-29 | 1985-12-04 | Method for the production of bead dextran materials for gel chromatography |
| EP85308833A EP0188084B1 (de) | 1984-12-29 | 1985-12-04 | Verfahren zur Herstellung von Schuppen auf Basis von Dextranmaterialien für Gelchromatographie |
| CA000497691A CA1263379A (en) | 1984-12-29 | 1985-12-13 | Method for the production of bead dextran materials for gel chromatography |
| US06/809,929 US4794177A (en) | 1984-12-29 | 1985-12-16 | Method for the production of bead dextran materials for gel chromatography |
| JP60293332A JPS61193068A (ja) | 1984-12-29 | 1985-12-27 | ゲルクロマトグラフイ−用粒状デキストランの製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS8410609A CS249368B1 (en) | 1984-12-29 | 1984-12-29 | Method of pearls detran materials production for gel chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CS249368B1 true CS249368B1 (en) | 1987-03-12 |
Family
ID=5448834
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS8410609A CS249368B1 (en) | 1984-12-29 | 1984-12-29 | Method of pearls detran materials production for gel chromatography |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US4794177A (de) |
| EP (1) | EP0188084B1 (de) |
| JP (1) | JPS61193068A (de) |
| CA (1) | CA1263379A (de) |
| CS (1) | CS249368B1 (de) |
| DD (1) | DD261297A3 (de) |
| DE (1) | DE3568336D1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SE452469B (sv) * | 1986-06-18 | 1987-11-30 | Pharmacia Ab | Material bestaende av en tverbunden karboxylgrupphaltig polysackarid och forfarande vid framstellning av detsamma |
| US5290774A (en) * | 1989-08-03 | 1994-03-01 | Eisai Co., Ltd. | Photostabilizing method for ophthalmic solutions and the resulting ophthalmic solutions therefrom |
| RU2187363C2 (ru) * | 2000-07-27 | 2002-08-20 | Институт биоорганической химии им. М.М. Шемякина и Ю.А. Овчинникова РАН | Способ получения гидрофильного геля |
| KR100457546B1 (ko) * | 2001-05-02 | 2004-11-18 | 주식회사 바이오프로젠 | 폴리프럭토오스 또는 그 유도체를 이용한 미소구 및 그제조방법 |
| US7473367B2 (en) * | 2002-06-26 | 2009-01-06 | Dionex Corporation | Monolithic column |
| US20050061745A1 (en) * | 2002-06-26 | 2005-03-24 | Teledyne Isco, Inc. | Separation system, components of a separation system and methods of making and using them |
| US6749749B2 (en) * | 2002-06-26 | 2004-06-15 | Isco, Inc. | Separation system, components of a separation system and methods of making and using them |
| EP2361968B1 (de) | 2010-02-26 | 2014-11-19 | Corning Incorporated | Synthetische Polysaccharid-Mikroträger zur Kultivierung von Zellen |
| US20120193367A1 (en) | 2011-01-27 | 2012-08-02 | Silgan Containers Llc | Heated container having chemical heating mechanism |
| CN104324708B (zh) * | 2014-11-05 | 2016-08-24 | 华玉叶 | 一种亲水色谱介质的制备方法 |
| GB201509677D0 (en) * | 2015-06-04 | 2015-07-22 | Ge Healthcare Bio Sciences Ab | Manufacturing process for polysaccharide beads |
| JP6972606B2 (ja) * | 2017-03-24 | 2021-11-24 | 昭和電工マテリアルズ株式会社 | 分離材、カラム、及び分離材の製造方法 |
| JP6926573B2 (ja) * | 2017-03-24 | 2021-08-25 | 昭和電工マテリアルズ株式会社 | 分離材及びカラム |
| CN113899842B (zh) * | 2021-12-08 | 2022-03-29 | 浙江湃肽生物有限公司深圳分公司 | 三肽-1铜的检测方法 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1292883B (de) * | 1958-04-16 | 1969-04-17 | Pharmacia Ab | Polymeres, in Form von in einem Loesungsmittel gequollenen Gelkoernern vorliegendes Trennmittel |
| US3042667A (en) * | 1959-03-10 | 1962-07-03 | Pharmacia Ab | Process for producing dextran derivatives and products resulting therefrom |
| SE358894B (de) * | 1961-10-25 | 1973-08-13 | Pharmacia Ab | |
| GB1087964A (en) * | 1966-04-15 | 1967-10-18 | Gordon Owen Shipton | Process for the preparation of cross-linked dexaran in the form of beads |
| US3865807A (en) * | 1971-12-03 | 1975-02-11 | Canadian Patents Dev | Aromatic dextran derivatives |
| US4293654A (en) * | 1977-10-17 | 1981-10-06 | Massachusetts Institute Of Technology | Cell culture microcarriers |
| SU1002356A1 (ru) * | 1980-12-31 | 1983-03-07 | Всесоюзный кардиологический научный центр АМН СССР | Способ получени иммобилизованного фибринолизина |
| SE8103137L (sv) * | 1981-05-19 | 1982-11-20 | Pharmacia Ab | Polymer med kvartera aminogrupper |
| JPS58137752A (ja) * | 1982-02-09 | 1983-08-16 | Japan Exlan Co Ltd | 液体クロマトグラフイ−用ゲルの再生方法 |
| US4507472A (en) * | 1983-06-14 | 1985-03-26 | Usher Thomas C | Manufacture of diethylaminoethyl dextrans |
| SE441363B (sv) * | 1984-01-23 | 1985-09-30 | Stellan Hjerten | Metod for tverbindning av agarprodukter i alkalisk miljo |
-
1984
- 1984-12-29 CS CS8410609A patent/CS249368B1/cs unknown
-
1985
- 1985-11-13 DD DD85282879A patent/DD261297A3/de not_active IP Right Cessation
- 1985-12-04 DE DE8585308833T patent/DE3568336D1/de not_active Expired
- 1985-12-04 EP EP85308833A patent/EP0188084B1/de not_active Expired
- 1985-12-13 CA CA000497691A patent/CA1263379A/en not_active Expired
- 1985-12-16 US US06/809,929 patent/US4794177A/en not_active Expired - Fee Related
- 1985-12-27 JP JP60293332A patent/JPS61193068A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP0188084A3 (en) | 1986-11-26 |
| US4794177A (en) | 1988-12-27 |
| DE3568336D1 (en) | 1989-03-30 |
| EP0188084A2 (de) | 1986-07-23 |
| DD261297A3 (de) | 1988-10-26 |
| JPS61193068A (ja) | 1986-08-27 |
| CA1263379A (en) | 1989-11-28 |
| EP0188084B1 (de) | 1989-02-22 |
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