CS246590B1 - Fermentor - Google Patents

Fermentor Download PDF

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Publication number
CS246590B1
CS246590B1 CS832662A CS266283A CS246590B1 CS 246590 B1 CS246590 B1 CS 246590B1 CS 832662 A CS832662 A CS 832662A CS 266283 A CS266283 A CS 266283A CS 246590 B1 CS246590 B1 CS 246590B1
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CS
Czechoslovakia
Prior art keywords
partition
membrane
cavity
aerator
vessel
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CS832662A
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English (en)
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CS266283A1 (en
Inventor
Jurij V Redikulcev
Leonid A Litvinenko
Tajsija S Cermenskaja
Svetlana B Petrikevic
Michail G Maximov
Original Assignee
Redikultsev Jury V
Leonid A Litvinenko
Tajsija S Cermenskaja
Svetlana B Petrikevic
Michail G Maximov
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Application filed by Redikultsev Jury V, Leonid A Litvinenko, Tajsija S Cermenskaja, Svetlana B Petrikevic, Michail G Maximov filed Critical Redikultsev Jury V
Publication of CS266283A1 publication Critical patent/CS266283A1/cs
Publication of CS246590B1 publication Critical patent/CS246590B1/cs

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Description

HasBatuie Η3θ6ρετεΗΗπ: ΦΕΡΜΕΗΤΕΡ Μβοδρετεκκε othochtch k annapaTaM pjw περΗΟΑΗπεεκοΓο BbipanpiBaHHH paanHH-Hbix KyjibTyp MHxpoopraHH3MOB Ha xhakhx, TBepflbix ηπη CMemaHHbix cyCcTpaTax hMOX8T 6bITb HCnOJIbSOBaHO B MHKpo6HOJIOrHHeCKOň, nHmeBOň, MeflHIJHHCKOň Η XHMH-qecKoft oTpacjiax προΜΜπιπεΗΗοετΗ, a Taxxe b HayHHo-HccjieflOBaTenbcxoň npax-THxe. fljlH KyjIbTHBHpOBaHHH ΜΗΚρθθρΓ3ΗΗ3ΜΟΒ, a ΗΜβΗΗΟ ΓρΗ0ΟΒ, MHKpOBOflOpoéjieftηπη oTflenbHoň xynbTypbi TKaHH, b ΜΗκροδΗοποΓΗΗεεκοή πρβκτκκε πιπροκο ncnonb-3ywT xHMHHecxHe “Kon6bi, yxynopeHHbie βητημμ Φηπβτρομ, b kotopmx pasMenteH 6h-ojiorHwecKHň ο6ΐεκτ, a ero BbipantHBaHHe προβοαητ Ha TepMocTaTHpyeMoň xanan-κβ -[1] .
OflHaxo HecMOTpa Ha yHHBepcanbHocTb κοπ6, ncnonb3OBaHne hx orpammeHo bnaSopatopHoň npaxTHKe H3-3a cnaóoro Maccoo6M8Ha. ΚροΜβ τογο, κοπ6μ Ηβ npH-CnOCOGjieHbl ΑΠΗ BbipaiUHBaHHH MHKpOOpraHH3MOB Ha TBepflblX ΠΗΤεΤ8ΠΒΗΜΧ cyScTpa-Tax. H3BBCTHbi φβρΜβΗτερΜ, Kaxflbiň H3 xoTopbix o6naAa8T rHflpoAHHaMHHecKóň xapax-TepHCTHxoň, oSecne^HBaraiueň ηκτηβημΑ poct Toň ηπη HHoň xynbTypw MHxpoopra-HH3MOB [2] .
OflHaKo Hcnonb3yeMbie πβρβΜβωΗΒβιοπριβ ycTpoftcTBa b sthx φερΜεΗτερεχ o6pa3y·-IOT CHHbHOe ΒΟΠβΗΗΒΗΗΗβ KynbTypanbHOň XHAKOCTH, a flnH ΠρβΑΟΤΒρβΠ,βΗΗΗ neHblb hhx Hcnonb3yeTCH xHMHwecKoe ηπη MexaHHHeČKoe neHoraweHHe, πτο βπηηβτ haxapaKTepHCTHKy npoTeKawmero npouecca. KpoMé toto, yKaaaHHbie annapaTbi o6ec-nenHBawT BbipamHBanne οαηογο ηπη AByx bhaqb μηκροοργηηηθμοβ, HanpHMep apox-xeň ηπη CaKTepHň.
HanSonee 6πη3Κημ κ Η3θ6ρετεΗΗΐο τεχΗΗπεεκΗΜ ρεπιεΗΗεΜ hbhhbťch φερΜεΗτερ,KOTopbiň coAepxHT 8MKocTb, cHa6x8HHyio τεπποοδΜεΗΗοή pyOaniKoň h pa3MamaHHbiMB Βερχκεΰ εε qacTH 6aKT8pHanbHbiM φΗΠΒτροΜ eosAyxa, aapaTop c 3a6opHoň Tpy6-Kofi H yCTpoňcTBO ΑΠΗ ΠερεΜ811ΙΗΒΗΗΗΗ, ΒΜΠΟΠΗεΗΗΟε B ΒΗΑβ MBMSpaHbl, yCTaHOB-πεΗΗοή b ΠΗεΒΜΟ,κβΜερε [3] . ΦερΜεΗτερ H3B8CTHOň KOHCTpyKAHH Μοχετ 6bITb HCnonb3OB3H TOAbKO ΑΠΗ Bbipa-IHHBaHHH 6aKTBpHH ΗΠΗ ΑΡΟΧΧεή Ha XHAKHX nHTaTanbHblX ερεΑβΧ. 246590
Hejibw H3o6peTeHHH hbjihstch pacinnpeHne φγΗκιμιοΗΗΛΕΗΗχ βο3ΜΟ)κηοοτθη anna-paTa πγτεΜ BbipauiHBaHHH pa3JiniHb!x bhaob KyjibTyp μηκροοργηηη3Μοβ na jkh/ikhxhjih TBepflbix, hjih cMeuiaHHbix cy6cTpa'rax. YKa3aHHaH nejib nocTuraeTCH τεΜ, ητο b φερΜβΗτερε, coneprcameM βμκοοτβ,cHa6«enHyro τθπβοο6μθηηοη pySauiKOH n pa3MemeHHbiM b BepxHeň ee nacra 6aKTe-PHajibHbíM φίΐπΒτροΜ BO3nyxa, aapaTop c 3a6opHoň τργδκοή h ycTpoňcTBo hjih ne-peMeinuBanHB, BbinonHeHHoe b BH/je MeMSpaHbí, ycTaHoBJíeHHoň b nneBMOKaMepe,riHeBMOKaMepa OTflejieHa ot nojiocTH θμκοοτη neperopoflKoň c no MeHbiuen Mepeoahhm OTBepcmeM, pacnonoHceHHbíM 3KcneHTpHMno och eMKOCTH, πρκ stom aaparoppaaMemeH Ha neperopoflKe h coflepacHT xaMepy hjih pacnpeflejieHHH raaoBbix h jKHfl-KOCTHblX ΠΟΤΟΚΟΒ, CHa6«eHHyiO yCTaHOBJlGHHblMH ΠΟ ee BbíCOTe flByMH oBpaTHblMHKJianaHaMH h pasMemeHHbíM Meatny hhmh pacnopHbíM kojibuom c οκηημη /via coo6nie-hhh nonocTH, pacnonoaceHHoň Me«ny oBpaTHbíMH KJianaHaMH c HaflMeMCpaHHoň hojio-CTbio nHeBMOKaMepbi, npHneM b CTeHKe KaMepbi BbinonHenbi oTBepcran fljia cooSqe-hhh noflKJianaHHoň nonocTH c nonocTbio gmkocth.
IlpHneM b eMKOCTH annapaTa ycTaHOBJíena ropH3OHTajibHan neperopoflKa c ot-BepcTHHMH flJiH pa3MemeHHH TBepfloro cyĎcTpaTa.
Ha Φηγ.1 cxeMaTHHHo H3o6paa<eH φβρΜβΗτερ; Ha Φηγ.2 h 3 - to me, BapHaHTbiBbinOJlHeHHH . ΦερΜεΗτερ coflepacHT eMKoeTb 1 c TenjiooBMeHHoň pyBauiKoň 2, b BepxHeň qac-th κοτοροβ ycTaHOBJíeH 6aKTepňajibHbiň φΗη&amp;τρ 3 BO3nyxa, a no och gmkocth 1ycTaHOBJíeH aapaTop 4 c 3a6opHoň τργθκοή 5. EmkoctL· 1 CHaSweHa ycTpoňcTBOMfljiH nepeMeniHBaHHH, BbinojiHeHHbíM b βηηθ MeM6paHbi 6, ycTaHoBJíeHHoň b nHeBMO-KaMepe 7 co uiTynepoM 8. ÍlHeBMOKaMepa 7 οτ ποποοτη eMKOCTH 1 oTflejieHa nepe-ropoflKoň 9 c no MeHbiueň Mepe οπηημ oTBepcTHeM 10, pacnojiosceHHHM SKcneHT-pHHHO och eMKOCTH 1. AapaTop 4 pa3MemeH Ha neperopoflKe 9 h conepacHT KaMepy11 fljin pacnpefleneHHH ra3OBbix h atHflKocTHbix ποτοκοβ, cHaSxeHHyw ycTaHOBjíeH-HbiMH no ee BbicoTe πβυμη oOpaTHbíMH KJianaHaMH 12 h 13 h pa3MemeHHbiM Meacflyhhmh pacnopHbíM KOJibnoM 14 c OKHaMH 15 ffJiH cooOiueHHH nonocTH, pacnojiojReH-Hoň Meacfly oBpaTHbíMH KJianaHaMH c HanMeMčpaHHoň nojiocTbio KaMepbi 11, npHneMb CTeHKe KaMepbi BbinonHeHbí OTBepcTHH 16 h 17 ajih coo6ineHHH noflKJianaHHoň no-jiocth c nonocTbio eMKOCTH. EmkoctL· 1 ΗΜβετ repMeTH3HpoBaHHoe ynnoTHeHHe 18.BHyTpH eMKOCTH 1 annapaTa Ha 3a6opHoň τργδκε 5 aapaTopa 4 ycTaHOBJíeHa ro-pH3OHTajibHa« neperopoflKa 19 c oTBepCTHHMH 20 hjih pa3MemeHHH TBepnoro cy6-CTpaTa, ynepacHBaeMaH Ha aapaTope 4 c noMombio ynjioTHHTejibHbix KOJien 21.
CornacHO BapnaHTy φερΜβΗτερβ (Φηγ.2) aapaTop 4 cHaSaceH CTpyflHbíM ΗΗ»βκ-τοροΜ 22.
CornacHO BapHaHTy φβρΜβΗτβρβ, H3o6pa5KeHHOMy Ha Φηγ.3, aspaTop 4 cHaó-aceH οφερΗΗβοκΗΜ OTpaacaTeneM 23.
OepMeHTep pa6oTaeT cjiegyrauiHM o6pa3OM. B HcxoflHOM coctohhhh (Φηγ. 1) oSpaTHbie KjianaHbí 12 h 13, pacnonojKeHHbie bKaMepe 11 jjjih pacnpenejieHHH ra3OBbix h «hakoctuux ποτοκοβ, ycTaHOBJíeHbí bHanpaBJíeHHH hjih npoTOKa BO3nyxa H3 eMKOCTH 1 b HaflMeMBpaHHyro nonocTb πηθβ-MOKaMepbi 7 h c BO3BpaTOM βτογο BO3flyxa b nojiKJianaHHyio nonocTb eMKOCTH 1.
Ilepefl HananoM pačoTbi φθρΜθΗτερη, chhb SaKTepHajibHbiň φκπΕτρ 3, bhochtKýnbTypanbHyio wnflKOCTb c thkhm pacneTOM, ητο6μ aspaTop 4 HaxoflHjicn BbnneypOBHH 3T0H JKHflKOCTH, a 3aTeM BHOBb 3ΗΚρΜΒ3Κ)Τ eMKOCTb 1 BaKTepHajlbHblM $HJlb"τροΜ 3 η b nHeBMOKaMepy 7 nonaioT cscaTbiň Boanyx c noonepeflHbíM cSpocoM ero .b θΤΜΟοφεργ. ΠρΗ nocTynneHHH cacaToro BO3flyxa b nHeBMOKaMepy 7 MeMÓpaHa 6noflHHMaeTca BBepx no coynapeHHH c neperopoflKoň 9, πρκ stom BO3flyx BbrrecHH-eTC« H3 HaflMeMOpaHHoň nonocTH nHeBMOKaMepbi 7 qepe3 OTBepcTHe 10 h 16 h ne-pe3 oKHa 15 pacnopHoro Kojibna 14 h KJianaH 13 c ero najibHeňiuHM peaKHM bh-Bpocom nepe3 OTBepcTHH 17 b nonocTb eMKOCTH 1. 246590 Πρκ peaKOM B«6poce B03flyxa b KyjibTypajibHyio jKHflKocTb b Heft npoHcxoRHTpacTBopeHne KHCJiopofla βτογο Boaflyxa, mto ηθο6χοαημο πρκ βμρηπϊηβηηηη aspoC-HblX MHKpOOpraHHBMOB, OTfleJIbHoft TK3HH HJIH MHfleJIHH, ΚρΟΜβ ΤΟΓΟ, 3T0T BOSflyXnoflHHMaeT ypoBeHb KyjihTypaJibHoň jkhakocth b θμκοοτη 1, xoTopas, b cboio one-pe«b, ΒωτεϋΗΗβτ nacTb BO3flyxa, HaxoflHmerocH b naflXHRKocTHoň nonocTH θμκο-οτη 1, uepe3 6aKTepnanbHbiň φκπχ>τρ 3 b aTMoc$epy. 3aTeM cacaTbift B03Ryx H3 riHeBMOKaMepbi 7 nepe3 πιτγμβρ 8 c6pacbiBaeTca b aT-Mocifcepy h MeMSpaHa 6 BO3BpamaeTCH b HcxoflHoe nojioaceHHe, oflHOBpeMeHHo aaca-cbraaH BO3flyx H3 HanwHAKOCTHoft ποποοτη, a TaKjtce H3 3ΤΜοοφβρί>ι nepea φκπΒτρ3,a3paTop 4, aaSopHyio TpyCxy 5, oSpaTHwň KJianaH 12, okhh 15 pacnopHoro kojib-fla 14 h OTBepcTHH 16 h 10 b HaRMeM6paHHyio nonocTb ππβΒΜΟΚβΜβρω 7. flajiee sth αηκπμ moscho ποβτορητβ c aaflaHHoň nacToToň Ha προτη»θηηη BceronpOUeCCa BbipanjHBaHHH μηκροοργηηηβμοβ. fljlH npOBefleHHH npOfteCCOB BbipaiUHBaHHH MHKpOOpraHH3MOB HJIH MHfleJIHH naTBepjjoM cy6cTpaTe, HanpHMep conoMe, onHJixax hjih yrne, b φερΜβΗτβρβ Ha aa-SopHoň Tpyóxe 5 aspaTopa 4 c πομοήβιο ynjioTHHTejibHbix KOJiefl 21 ycTaHHBjíHBaioTropH30HTajibHyio neperopoflKy 19 c οτβθροτηημη 20, na κοτοροή pa3MemaioT TBep-flbiň cyScTpaT. fljin noflflepscaHHH Heo6xoflHMoň BJiascHocra cy6cTpaTa nofl ropH3OH-TajibHyio neperopoflKy 19 3ajiHBaioT Bony hjih nHTaTejibHyio 'cpefly, a aspaipno πρκT3kom npoflecce προβοαητ anaJiorHHHo onHcaHHOMy npoyeccy BbipancHBaHHH mhkpo-opraHH3MOB na hchrkom cyócTpaTe.
CorjiacHO BapnaHTy (Φηγ.2) annapaT paBoTaeT cJieflyiomHM o6pa3OM. 06paTHbie KJianartbi 12 h 13 o6ecnenHBaioT προτοκ KyjibTypajibHoň jkhrkocth bKaMepe 11 H3 θμκοοτη 1 b HaflMeM6paHHyio nonocTb nHeBMOKaMepbi 7 h BO3BpaT3TOH JKHRKOCTH B eMKOCTb 1 Ηθρθ3 CTpyftHblft HHSCeKTOp 22. Πρκ nocTynjieHHH cacaToro B03flyxa b nHeBMOKaMepy 7 MeMSpaHa 6 ποαηημηθτοηBBepx flo coyflapeHHH c neperopoflKOň 9, πρκ stom KyjibTypajibHan JKHflKocTb Bbi-TecHHeTCH H3 BepxHeň nonocTH πηθβμοκημθρμ 7 flepes οτβθροτηη 10 h 16 h ok-Ha 15 pacnopHoro,, KOJibfla 14, KJianaH 12 h 3a6opHyra Tpy6ny 5 aapaTopa 4, Ha-THeTaeTCH β ΗΗΐκεκτορ 22, nocjie nero BO3BpamaeTCH b BHRe crpyn b KyjihTy-paJibHyio JKHRKocTb θμκοοτη 1 . CTpyn, npoHH3biBaH KynbTypajibHyio jehrkoctb, rhc-neprHpyiOT B Heft BO3flyX, KOTOpblft 3aÓHpaeTCH HMH H3 HaflSCHflKOCTHOft nOJIOCTHeMKOCTH 1. ΠρΗ cSpoce cacaToro B03flyxa H3 nHeBMOKaMepbi 7 nepe3 nrryflep 8 b aTř-rc^epyMeMfípaHa 6 BO3BpamaeTCH b HcxoflHoe nojioscenne, οαηοβρθμθηηο 3acacbiBaH Kyjib-TypajibHyio schrkoctb H3 θμκοοτη 1 nepe3 οτβθροτηθ 17, o6paTHbiň KJianaH 13 ok-Ha 15 pacnopHoro KOJibfla 14 η οτβθροτηη 16 h 10 oOparao b HaflMeMčpaHHyw no-nocTb nHeBMOKaMepbi 7. flanee sth αηκπμ ποβτορηκτοη c aaflaHHoň nacTOToň.
CorjiacHO KOHCTpyKTHBHOMy BapnaHTy (Φηγ.3) annapaT paCoraeT aHajiormHbíMo6pa3OM, πρκ 3TOM οφερΗΗβεκΗή oTpajKa.Tejib 23 HanpaBJíaeT KyjihTypajihHyro jrhr-KOCTb ria οτθηκη θμκοοτη 1 b BHfle njieHKH fljia pacuiHpeHHH CBeTOBOň · noBepXHO-CTH npH BbipamHBaHHH φοΤΟΗΒΤΟΤροφΗβΓΧ ΜΗΚΡΟΟΡΓΗΗΗ3ΜΟΒ . Πο cpaBHeHHio c Η3βθοτημμη npefljiaraeMbiň φβρΜβΗτερ ημθθτ yHHφH^HpoBaHHbley3Jibi, KOTopue b pa3JiHHHbix KOM6HHan;HHx πο3βοπηιοτ npoBORHTb BbipamHBaHHe paa-JIHHHbIX KyjIbTyp MHKpOOpraHH3MOB (flpOJKJKeft, SaKTepHH, rpnCOB H OTfleJIbHoftKyjihTypbi τκηηη) Ha schrkhx hjih TBepflbix, hjih CMetuaHHbix cy6cTpaTax. TaKoňnofl-xor κ penieHHio npoBjíeMbi HCKjnonaeT πρΗοδρετβΗΗβ floporocTonmero φβρΜβΗΤΗΑΗοκ—’noro o6opyflOBaHH« h no3BOJiaeT 3HaqHTejibHo coKpaTHTb BpeMfl Ha o6pa6oTKy Tex-ηοπογηη npOBORHMbix npoijeccoB. 246590 4 ΦΟΡΜΥΠΛ Η3ΟΒΡΕΤΕΗΗΗ 1. ΦβρΜβΗτερ, coflepjKaiHHň eMKocTb, CHaSweHHyio TennooBMeHHoň pySaniKoňη pa3MemeHHbiM b BepxHeA ee nacTH SaKTepHanbHbíM Φηπβτρομ BO3p,yxa, aapaTopc 3a6opHoň TpyGKoň h ycTpoňcTBO nna nepeMeniHBaHHa, BbinojiHeHHoe b BHfle μθμ-SpaHbí, ycTaHOBJíeHHoň b πΗβΒΜοκβΜβρβ; OTJinqaiomHňcH τθμ, mto, cne π bio pacuiHpeHHH íiyHKUHOHanbHbix BO3MoxHocTeň φερΜβΗτβρβ πγτβΜ BbipamHBaHHHpaannHHbix βηοοβ μηκροοργβηη3μοβ Ha «ηακηχ hjih TBepňbix hjih CMemaHHbix cy6-CTpaTax, nHeBMOKaMepa oT^eneHa ot nonocTH gmkocth neperoponKoň c no MeHh-meň Mepe οληημ οτβθρθτηθμ, pacnonoaceHHbíM οκοηθητρημηο och θμκοθτη, πρηβτομaapaTop paaMemeH Ha neperoponxe h conep»HT xaMepy nna pacnpeneneHHH raao-BblX H KHflKOCTHbIX ΠΟΤΟΚΟΒ, CHaCweHHyiO yCTaHOBJíeHHblMH ΠΟ ee BblCOTe flByMH 06“paTHbíMH KnanaHaMH h pa3MemeHHbiM Me«ny hhmh pacnopHbíM KonbuoM c οκηβμη xvihcoo6meHHH nonocTH, pacnojio«eHHoň Meacjny ofipaTHMMH KJianaHaMH c HaflMeMGpaHHoflnonocTbionHeBMOKaMepbijnpHHeM b ετεκκβ KaMepw BbinonHeHbí οτΒβρετΗΚ flnn coo6-meHHH noflKnanaHHoň nonočTH c nonocTbio θμκοοτη. 2. ΦερΜθΗτβρ,πο n.1, oTnHnaioiUHňcH τθμ, ητο b θμκοθτη ycTa-HOBnena ropH3OHTanbHaH neperoponKa c οτβθροτηημη ππη pa3MemeHHH TBepnorocyScrpaTa. 246590 ΡΕΦΕΡΑΤ ΦΕΡΜΕΗΤΕΡ ΗβοβρετεΗκε othochtch κ annapaTaM ρ,ηα βμρηι4ηβηηηη xynbTyp MHKpoopraHHa-μοβ Ha jkhakhx, TBepflbK hjih CMemaHHbix cyGcTpaTax. ΦερΜΒΗτερ coflepacHT eMKOCTb 1, CHaČHteHHyio τεπποοβΜβΗΗοή pyCamKoň 2 nOaK“TepuajibHbSM Φηπβτρομ 3 Boanyxa, aspaTop 4 c 3a6opHoň rpyCxoft 5 h ycTpoňcTBoΛΠΗ nepeMemHBaHHH, BbmoJiHeHHoe b bhao MeMCpaHbí 6, ycTaHOBJíeHHoň b πκβΒΜΟ-KaMepe 7. flna pacmHpcHHH ýyHKijHOHajibHbix Βθ3Μο»Ηοοτεή φερΜεΗτερβ πηθβμοκβ-Mepa 7 OTfleneHa. ot πο/iocth cmkocth 1 neperopoflKoň 9 c otbbpcthhmh 10. lípu3tom aspaTop 4 pasMemeH Ha περετοροΑκε 19 h coflepwHT xaMepy 11 απη pacnpe-ACJichhh rasoBbix h «HAKocTHbix ποτοκοβ, KaMepa 11 cHaCweHa ο6ρβτημμη Knana-HaMH 12 h Í3 h pacnopHbíM xojibqoM 14 c οκηβμη 15 hjih cooSmeHHH nonocTH,pacnonoxeHHoň Ma»Ay ο6ρβτημμη KJianaHaMH 12, 13 c HaAMCMCpaHHoň nonocTb»nHeBMOKaMepbi 7. B CTeHxax xaMepbi 11 BbinojiHBHbí otbcpcthh 16, 17 ajih coo6-meHHH nonocTH nofl xnanaiiaMH c nonocTbio cmkocth 1. neperopoflxa 19 c otb6pcthhmh. 20 cnyxHT απη pa3MemeHHH τβθραογο cyOcTpa-Ta. 3 hji . IIpH3HaHo Η3θ6ρετβΗΗβΜ no pe3ynbTaTaM 3κεπερτΗ3ω, ocymecTBjíeHHoň Focyflap-ctbohhhm ΚοΜΗτετοΜ CCCP no flonaM Η3θ6ρετεΗΗ&amp; η οτκρμτη£. 3 περτεκε 246590

Claims (2)

  1. 246 590 P R B D M S T VYNÁLEZU
    1. Kvasná ká3, sestávající z nádoby, opatřené teplosměnnýmpláštěm a v horní části umístěným bakteriálním filtremvzduchu, aerátor se sací trubkou a míchací zařízení provedenéve tvaru membrány, umístěné v pneumatické komoře, vyzna-čující se tím, že S cílem rozšířit funkční možnosti kvas-né kádě na pěstování různých druhů mikroorganizmů na te-kutých, pevných nebo směsných substrátech, je pneumatickákomora (7) oddělena od dutiny nádoby (1) přepážkou (9) sjedním otvorem (10), umístěným výstředně od osy nádoby,přičemž aerátor (4) je umístěn na přepážce (9) a obsahujekomoru (11) k rozvádění toků plynu a kapalin, opatřenou nad sebou dvěma zpětnými ventily (12,' 15) a mezi nimi roz~pínacím kroužkem (14) s otvory (15) k vytvoření dutiny,umístěné mezi zpětnými ventily s nadmembránovou dutinoupneumatické komory (7)» přičemž ve stěně komory (11) jsou.provedeny otvory (16, 17) k vytvořeni dutiny pod ventilys dutinou nádoby (1).
  2. 2. Kvasná ká3 podle bodu, 1, vyznačující se tím, že v nádo-bě je uspořádána hotizontální přepážka (19) s otvory (20) k uložení pevného substrátu.
CS832662A 1982-10-10 1983-04-13 Fermentor CS246590B1 (en)

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US4727040A (en) * 1985-03-01 1988-02-23 New Brunswick Scientific Co., Ltd. Sparger for fermentation and tissue culturing vessels
DE3810843A1 (de) * 1988-03-30 1989-10-12 Michael Etzold Antrieb fuer volldurchmischte bioreaktoren
WO2019126654A1 (en) * 2017-12-22 2019-06-27 Cuello Joel L Axial dispersion bioreactor (adbr) for production of microalgae and other microorganisms
WO2020041762A1 (en) 2018-08-24 2020-02-27 Cuello Joel L Mobile and modular cultivation systems for vertical farming
CN109220557B (zh) * 2018-09-28 2023-11-28 天津绿圣蓬源农业科技开发有限公司 一种曝气效率高的瓶栽食用菌基质发酵装置
RU195650U1 (ru) * 2019-06-28 2020-02-03 Федеральное государственное бюджетное образовательное учреждение высшего образования "Красноярский государственный аграрный университет" Лабораторный ферментер
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US2146326A (en) * 1935-11-22 1939-02-07 Int Suiker En Alcohol Cie Inte Process for obtaining alcohol by fermentation of liquids containing carbohydrate
GB1151526A (en) * 1966-08-10 1969-05-07 Richard Whittington Friedel Improvements in and relating to Sound Systems
US3664770A (en) * 1970-02-18 1972-05-23 Golden Arrow Mfg Ltd Diaphragm pumps
CA929121A (en) * 1970-05-08 1973-06-26 National Research Council Of Canada Non-ramified culture growing apparatus
GB1331163A (en) * 1970-07-13 1973-09-26 Hitachi Ltd Method for aerobic fermentation
SU373292A1 (ru) * 1971-03-18 1973-03-12 У. Э. Вкестур, И. А. Стурманис, С. Э. Селга , М. Ж. Кристапсонс Институт микробиологии Августа Кирхенштейна ШйгЕШЕО-ТЕКОНЕОШ
ZA76812B (en) * 1975-04-29 1977-04-27 Farmaceutici Italia A laboratory fermenter
US4173516A (en) * 1976-07-15 1979-11-06 Chemap Ag Device for cultivating cells of animal and human tissues
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