CS230958B1 - Method of detection of bacteria and equipment to perform this method - Google Patents

Method of detection of bacteria and equipment to perform this method Download PDF

Info

Publication number
CS230958B1
CS230958B1 CS376180A CS376180A CS230958B1 CS 230958 B1 CS230958 B1 CS 230958B1 CS 376180 A CS376180 A CS 376180A CS 376180 A CS376180 A CS 376180A CS 230958 B1 CS230958 B1 CS 230958B1
Authority
CS
Czechoslovakia
Prior art keywords
nutrient medium
blood
kpobh
container
θηηθ
Prior art date
Application number
CS376180A
Other languages
English (en)
Inventor
Manfred Kunze
Martin Schambach
Werner Koehler
Klaus Mueller
Original Assignee
Manfred Kunze
Martin Schambach
Werner Koehler
Klaus Mueller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Manfred Kunze, Martin Schambach, Werner Koehler, Klaus Mueller filed Critical Manfred Kunze
Publication of CS230958B1 publication Critical patent/CS230958B1/cs

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A61J1/06—Ampoules or carpules
    • A61J1/065—Rigid ampoules, e.g. glass ampoules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Clinical Laboratory Science (AREA)
  • Biophysics (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

.. λ.
ÚŘAD PRO VYNÁLEZY A OBJEVY PV^ 1 « OSOB./POŠTA 1 pftit UTVAŘ REF VYRiZ ο ο ω« ο'Ο ! οο ·
-J *£**
i ι
Ha3BaHíw H3o6peTeHiifl Μθτολ η oóopysoBamie λλη βηηκηθηεη óaKTepuMEg
OforacTB npHMeHemig Η3οόρθτθ5ΕΗ ίΙδοόρθτθΗΕθ KacaeTCH MeTQna e otíopynoBaHEH jxjlh βηηβλθηεη das-Tepm®a πγτβΜ npraaoro B3htes kpobh h ηηΦηφϊροββηηη reMOKyjiB-Typ nps jmxopano^HHx coctohhehx ηθηοηογο reneta, a Taxse jyiaHa&IWHIiH 3a óaKTepHaZTLHHMH 3300ΐΖΓθΒΗΗΗΗΜΗ Η3Ββ0ΤΗ0Γ0 UpOHCXOJK—ΑΘΗΒΗ C ΤβΜ, ^TOtíH HaJXeSHO OnpeSeJIÍÍTB H JieHHTB 3adOJieBaHHH,onacHne jvih SH3HE. . Μθτοα e oóopynoBaHHe, npej^iaraeme, Ε3θΰρβτΘΗΕ©Μ, cJipsaT KaK jyniB3HTES KpOBIÍ ΉΘΛΟΒθΚΒ, T3K H RJIX KyZIBTEBHpOBaHEfl MEKp00praHK3-MOB 23 ΚρΟΒΕ ftUS LWpOÓEOJIOrHBeCKHX E ΚΤΙΕΗΕΉΘΟΚΗΧ OJIKTHHX ECCJIS-JÍOBaHHg.
XapaKTgpHCTBKa Ε3Βθοτηηχ τθχηηηθοκεχ pemeHEH
Ho JUiarEOCTEKe óaKTepWtíí E3B©CTHH HeCKOJILKO ΜΘΤΟ^ΟΒ E BEHOBodopysosaHEíi, κοτορκο, ojtfiaxo, óaxee wih Menee oranaioTca ot-tfeJILHHlZZ STanSMH EXE HX BapHaHTaME, a TaXHM OÚpá3OM Η &ΘΤ3ΛΗΜΗoóopysosaHHH. ' Πθ ΠροΟΠΘΚΤΘΜ H3B6CTHa CHCTQMa JUH reMOKýJIBTypH VacutainerB GOOTBeTOTBHH C KOTOpOfí UpOBpflHTCH CJiejiyEHHH© ΟΟΗΟΒΗΗΘ 3TaiHTHccJie,n;oBaHHH óai^Tepiwm: . . - Βθητιιθ κροΒκ: CHCTeMoS B3hthh κροΒΗ H3 βθηη naixneHTa depeTca KPOBB c no-MOUIBIO ΗΓΛΗ, 3aiHHHíeHHOS B ΟΟΟΤΒΘΤΟΤΒΗΗ C DE-OS 2332133, ΟΤΘ-piUILHOrO nuipima HHH Haóopa'&71fl B3HTHH KpOBH H3 ΒΘΗΗ naiíHeH“
Ta. B3HTaH κροΒΒ nonauaeT πρπΜΟ b οτβριυίΒΗΗβ cocya c shhko0 hh-TaTejE&HOH cpeaofi-CHCTeMH jvw peMOKyJiLTypH, KOTopaa npe^cTaB- . ΛΗΘΤ COdoS ΟΤΘΚ7ΙΗΗΗΗΘ TpydOHKH, φΗΘΚΟΗΗ Η7ΓΗ COCyHH pa3JIHH-hhx βΜκοοτβδ, 33κρητηθ pe3HHOBoS npoÓKOfí hjth MeTázunraeCKoSKpauiKoa. 3th βββθοτηηθ cocynH jvlh reMOKyjihTypH 33κρπτΗ οτβ-PhjilhoS προόκοβ; ecjiH npoÓKa ηθ cTepiuiLHas, Torna nepejí B3H- .τηθμ kpobh ee cjie#yeT τιι^τθλβηο odpadoTaTL Hé3HH$HHHpynuHMCpejíCTBOM. lípu BCTaBjreHHH cocy.ua c reMOKyjr&Typofí b κρθιμθηηθ jyia ηγληoncTeMa reMonyjiBTypH coejwaeTca c cHCTeMóÉ b3hthh kpobh.
Ilocjie npoKajiHBaHHH βθηη cocyE ηθ^ημηθτόη Ha οοηοβθηηθ aep&a-τθλη T3K, hto npoóKa npoTHKaeTca η τθμ caMHM noa ΑββοτΒΗθΜBaKyyMa κροΒΒ oTcacHBaeTCH hs βθηη. Πρπ HOHBJíeHHH κροΒΗ ΗΒΗΗΗΗΘΤΟΗ ΗΘΜΘΗΛΓΘΗΗΟΗ HHOKyJIHHHH HHE-koS nHTaTejiBHoS cpejajz. Ecjih noTpetíyioTCH eme aHajiH3H kpobh,το HecKOJiBKO cocynoB jyia rewoKyjiLTypH cjiesyeT noonepesHo3an0JIHHTL pa3JIHHHHMH HHTaTeJIBHHMH ΟρθβθΜΗ. - HosroTOBKa h ηηΦηηηροβηηηθ cydKyjiBTyp:
Ilocjre topo, Kaz cocyn jyin reMOKyjiLTypH dyneT ynaJieH H3 htjio-JíepKaT&amp;HH Η ΤΘΜ CaMHM ΟΗΟΤΘΜΒ B3HTHH κροΒΗ ΟΤβθΛΗΤΟΗ OT CHC-τθμη λπη reMOKyTiBTypn, a κροΒΒ ηητθηοηβηο nepeMeinaeTCH οsHHKofí miTaTeJiBHofi. cpefloft, ocymecTBHTCH HHKydHpoBaHHecocy-fla c reMOKyjiBTypoS npz 35 - 37° C kok noaroTOBKa κ ηηΦηηηρο-BaHH» cydKyjn>Typ. i -3- ΠρΗ npHCTpofiKe ΗΘ3ΘΚρΗΤ0Γ0 HJIH 38ΚρΗΤ0Γ0 ΒβΗΤΚΤΙΗΐχΗΟΗΗΟΓΟ ΠρΗ“ύποοοάτίθΗΖΗ odecnemiBaeTCH KyjiBTHBHpoBaHHe aapodHHX wih aii-θροόΗΗΧ MHKp00praHH3M0B; B 3aBHCHM0CTH OT HX pOCTa, KOTOpHÉcJie^yeT KOHTpoJiHpoBaTB nepe3 οπρβββΛβΗΗΗθ npoMeayTKH βρθμθηη, . ocymecTBJíHeTCH nocjie ηβηογο πογ.^τηθηηη wkoíí nuTaTejiLHoií cpe-ΛΗ m<)niíiípoBaiBíe cydKyjrBTyp Ha TBepnyio nHTaTejiBHyio cpejiy.
Hepe3 pa3JinBHHe npoMejsyTKH ΒρθΜθΗΗ mnpimoM .nepe3 CTepiuiLHyioHTJiy H3 cocysa %jix reMOKyjiBTypH depeTca HHKyÓHpoBaHHaH miTa-TejiLHaa khskoctb, cMeináHHaa c MHKpoopraHB3MaMH, πρηηθμ npodxa . cocysa npoTHKaeTCH. Ojtfia mra ηθοκοζγβκο TBepnax nHTaTeJiBHHx cpeji,HaHeceHHHx Ha ocodse imacTUHKH jwh nanncz Πθτριι h cocTOHUiHe npe-ΗΤ,ψΙΙίθΟΤΒΘΗΗΟ H3 arapa C pa3JIHHHHMH £H$$epeHHHpOBaHHHMH SOÓaBKa- - ΜΗ, ΗΗφΠΙξΗργΤΟΤΟΗ. IlOCJlé HHČDHHHpOBaHIÍH arapOBHX KyJIBTyp OHH emepa3 HHKyóupyioTCH. Οτκρ&amp;ββτβ cocyjíH c reMOKyjiBTypoS jw EHOKy-JLHITHH KpOBLK) MOSHO Ηθρθ3 ΟΠρθβθΛθΗΗΗΘ npOMe&amp;yTKH ΒρθΜΘΗΗ, Ha3-S, 6-11 Η 10 ίξθΗΒ HOCJie B3HTHH KpOBH. JIhuib nocjie pocTa MHKpoopraHH3MOB b cydícyjiBTypax mosho npoBo- :ΛΗτβ ηθτκηθ MBKpoóHazioriraecKiie Hccjre^oBaHHH pa3JiHHHHX opraHH3-MOB.
HejíocTaTOK θτογο ξ3βθοτηογο MeTOsa 3aicjnoHaeTCH b tom, hto ot-ΛΘλιβηηθ 3Tanu b3hthh kpobh η ηηΦεηιιροβηηκθ reMOKyjiBTyp bíhigti-HíHOTCH C nOMODtbD HeCKGWIBKHX HpHÓOpOB, T3K ΗΤΟ ΗΘΤ rápaHTHH Πρθ-SOTBpanjeHKH 33ΓρΗ3ΗΘΗΗΗ πρκ B3HTHH Η ΠρΗ HH^HHHpOBaHHH H 3ace-βθ KyjiLTyp. .
Ho cHCTewaM jyin reMOKyjiBTyp, fleScTBywix no 3TOMy μθτο^ογ, H3-ΒΘΟΤΗΗ CTOKJIHHHHO COCy^H flJIH reMOKyjiBTyp C ρθ3ΗΗΟΒΟΗ IWH ΜθΤβΛ-jnnecKOH προύκοπ h HanoJiHeHHHe seskoS miTaTejiBHOH cpejxofi. ’·. .JUa Β3ΗΤΙΪΗ KpOBH H3 B6HH H HHOKyjIHIXHH ΚρΟΒΒΚ), a TaKSe B£y-BaHHH ra3ooópa3HHx BeinecTB b cHCTeMy jyiH reMOKyjiBTypH π jvlh. HHČJIfflHpOBaHHH SEEKHX HHTaTeJIBHHX Ορθβ flJIH CyÓKyJIBTyp ΠΟΟΤΟΗΗΗΟTpeóyeTCH προτκκβκΗβ htth οτκρηββηηθ cocysoB jw reMOKyjiBTypa.
Bo BpeMH 3thx sTanoB h ocodeHHo Bo Bper.iH onepamtíi c cydKyjiLTy-poií B JiadopaTOpHH B CBH3H C ΠροΤΗΚΒΗΗΘΜ WIH ΟΤΚρΗΒΒΗΗΘΜ npodOK,a TaKse npH MaHHnyjiHpoBafíHH mnpHHaMH, inviaMH ηθ ιιοκώοηθηο sa-ΓρίΙ3ΗΘΕΣΘ. - 4 --
JlpyroS HojíocTaTOK sroro Η3Βθοτηογο MeTo&amp;a coctoht b tom, htocjiaó.o pacTyujne kojiohhh όβκτβρπδ e^Ba mojkho odHapyjKHTB KaK bjkhukoh nHTaTejiLHoS cpeae, tbk' h Ha TBepnoM arape. Πο .npyrHM npocneKTaM H3BecTHH cocyflH jveh reMOKyjiLTypa XByx$a3-.HOH ΟΗΟΤΘΜΗ, T.H· CHCTeMH Gibco-Bio-Cult C ΗΘΟΚΟΛΒΚΗΜΗ ΠΗΤΗΤΘΛΒ-hhmh cpe#aMH, odJia£aiomne cneííHajiBHOH προΰκοίί h neperopojiKofi,pa3^eJiflKinea JKHHKHe h TBepnne πκτβτθλβηηθ cpejcn. 3th oocysu jyiareMOKyjiLTypti He #onycKaioT 3aceB npod juih cytíKyjiBTyp b paajnra-ΗΗΘ HHTepBazni ΒρβΜΘΗΗ H TOM CaMHM HOBTOpHOe ΟΤΚρΗΒΒΗΙίθ COCyHOB.Hwaa nHTaTejiLHaji cpe^a, cMemaHHaH c kpoblio, οτοκβθτ πρπ Bpa-meHHH cocyna b npejuiasHa^eHHyio &amp;τιη 3Toro KaMepy, ομηββθτ h hh-$nnnpyeT TBepwyio arapoByio $a3y, HaHeceHHyro Ha neperopoíCKy, hnocjie oToro ohhtb cJiHBaoTCH b npesHa3HaHeHHyio jyw θτογο KaMepycocyna.
HeaocTaTKOM θτοη ,HByx$a3HoS chctomh jvih reMOKyjiLTypu ηβττηθτοηto, hto b CBH3H c KOHCTpyKiínea cocyEa, HMeromero οτηοοιιτθλβηοÓOT&amp;myiO θΜΚΟΟΤΒ, ΗθΤ HpHMOS CBH3H MeJKJQT BeHOÉ, CHCTeMO0 B3HTHHKPOBH H CHCTGMOS flJLH reMOKyJIBTypH. Β3ΗΤΗβ KpOBH ,H3 ΒΘΗΗ, a 3Ha-HHT H-HQUana KPOBH B 3TOT COCyA HHH ŽJiaKOH JUH reMOKyjTBTypHocymecTBJífleTCfl, γλθβηημ odpa3OM, mnpHuoM tojilko cocysoB cHedOJTBIIIOH eMKOCTBÍO HOCpeaCTBOM nmaHTOBOrO COeaHHeHHH c uruioS Β3ΗΤΗΗ KpOBH Η7ΓΗ ΚΟΜ&amp;ΖΓβΚΤβ RXX Β3ΗΤΗΗ KpOBH.
HecMOTpn Ha to, hto sth j£Byxč&amp;a3HHe čmaKOHH λλη reMOKyjiBTypodecneHEBaioT doJiee cKopaS ροοτ kojiohhh na arape, a kpobb depeT-ch οτηθνΤ&amp;Ηο, BO3HHKaeT onacHocTB 3arpH3fíeHHH. Jlajiee, npn τεκοδcHCTeMe jvlh reMoKyjiBTypH saTpynHHeTca οπτηηθοκοθ oTpaseHne od-pa30BaHHH kojiohhh bo BpeMH HHKydaiw, a b cbh3H c sthm ocodeH-ho 3aTpynHHeTca MURpodHO^rorinecKoe HccJieflOBamie cjiadopacTymiixKyjiBTyp. IIoaTOMy HeodxoflHMO πρπ onpe^ejreHHHX BHnax reMOKyjiBTypTBepnyio arapoBys &amp;a3y ποβτορηο oMHBaTB nepe3 onpeaejreHHne npo-MesyTKH BpéMeHH 3KHHKOS. CMeCBK HHTaTeJIBHOS Cpe,HH H KPOBH. Πο DĚ-O3 2221096 h DE-os 2332133 b KanecTBe odopysoBaHHH jpwB3HTHH KpOBH Ι13ΒθΟΤΗΗ HHCTpyMeHT B3HTHH KpOBH H HTJia JtflH B3HTHHaH3JIH30B KpOBH.
CorjiacHo Ha3BaHH0My MeTojny b3hthh kpobh HHCTpyMeHT jj;jih B3hthhCOCTOHT H3 ΗΓΛΗ C flepftaTOJieM, npiraéM ΚΟΗΘΗ HTJIH, HaXOAHiqHSCH BHyTpH, OKpyHeH KJianaHOM C ΒβΖΤΖΤΙΖΖΖΟΖΗΗΜ ΠρΖΟΠΟΟΟ&amp;ΙβΖΖβΜ.:Knanaz htjth mokzo nepeBonzTL Z3 onzoro zojiosezzz, b κοτοροΜ .oóecnezzBaeTCz ΒθζτζτιζίίΚΗ (zepe3 yzKzfi zpoxon) z nóKa3 'κροεζ,b apyroe, b κοτοροΜ npenoTBpainaeTGH npoxosnezze B03nyxá mizκροΒΖ. .HepsaTeJiB. coctoht H3 ockoezoh zacTz b Bzne TpydozKK z zacTz cBHyTpeHHefí KOHiraecKOĚ noBepxHocTBD. Tpydozza cjiyszT jmz zpen-nezzz cocyna, no KOTopoiiiy 3BaKyzpyeTCH reMOKynBTypa, c προτΗ-Kaer.ioS ζροόκοζ. KozzzecKaz zoBepxzocTL cjiyszT b zazecTBe yno-pa jvlh cocyna c KyjiETypoz, Korná zozez htjih, npoTHKaiomzii npoó-Ky, προτκζβτ ee. CHCTeMH B3ZTZH ΚρΟΒΗ Ζ3ΒΘ0ΤΖΗΜ 0Ópa30M ΟΟβχΐίΗΗθΤΟΗ C CZCTeMOZjyiz rewoKyjiBTypH c homoiulio θτογο κρθιυίθΗζζ. - ΠοηοόΗΗΜ se oópaaoM ycTpoeHa zrjia, 3amwHzaH b οοοτβθτοτβζζc de-os 2332133; zepenHzz zacTL zrna ΒΒοηχτοζ b BeHy, a προτζ-BononosHaa zacTL - b codzpaTenLHHfí cocyn jw reMOKyjrLTypa. 3Tazrna OTJizzaeTcz oazoxozobhm mianaHOM, npenoTBpamajamHM BO3BpaTshhkocth Z3-coózpaTeJiBHoro cocyna b czcTewy KpoBooópameHZH za-HzezTa, a Tarase cpencTBOM ηπζ Harjrznzoro noza3a nBzseHZH κροΒΖ.y zrna zr.ieeTcz Tarase nepsaTem,, zpzcnocoájreaHHfi κ čoopMe coóz-paTeJiLHoro cocyna jpra reMOKyjiLTypa,- npencTaBJBnonrzH codoS npez-MymecTBeHHO nojmfi zznzzup jvlh KperaieHzz 3τογο cocyna. IlocjrenpoKona Beza οζοτθμη b3htzz kpobh coenzHzeTcz c οζοτθμοη nnHreMOKyjE&amp;Typa nocpencTBOM zpeznezza. HenocTaTKOM θτογο coenzHe-zzh, Tarase Kaz z za3BaHZoro βηιιιθ, .zBJízeTez to, zto yBeJizzzBa-θτοη 3arpfl3HfleM0CTB Bcez,οζοτθμη, a Tarase cjioszoctl nanL3OBa-HHH yCTpOHCTBaMZ. Πο de-ps III0357 zaBecTHH Bazypme aMnyjia jw CTepzzLzoroB3HTZH κροΒΖ z npyrzx szHKocTez; menza, 3azpaTaz BBepxy, za-caseza na zpoózy c zrnoK, Bnamiezzyio c zzsHero zozua aMzyna,z zrjiy; zz3 btzx ai-myji sazjiasjízeTcz non bhcokzm BazyyMOM. 3tz BaKýyuHHe aMnyjia cnysaT zczmozzTeJiLHo jyra bsztzh kpobz;OTcocazzaz κροΒΒ nojesHa nocne θτογο óhtl BBeneHa b oTnenEzyK)czcTewy jyw reMozyjiLTypH. ' f - 6 -
IfeftB ΙΙ300Ρ6ΤΘΗΗΗ IJeJILK) H30ÓpeTeHHH HBJIHGTCH C03flaHHe OtíopySOBaHHH jyífl HCCJie,HO-BaHHH' daKTepwnS, y κοτοροΓο ycTpaňeHH He^ocTaTKn η.3βθοτηηχμθτολοβ h odopyaoBamw, 3HaHHTeJiLHO yBeJiiraiTCfl npe;nynpewHHe3arpH3HeHHH, yiipOCTHTCH B3HTHH KpOBH H npHBHBaHHe KyJIBTyp daK—Tepjafí z yMeHBiuHTCH £0 chx nop bhcokhS pacxojí Ha TexHincy jyinCHCT6MH B3HTHH KpOBH H reMOKyJTBTyp.
IfejioaeHKe oymHooTH H3odpeTeHHg B cbh3H c sthm B ocHOBy Η3θόρθτθΗΗΗ ncwioaeHa 3a#ana pa3patío-THTB MeTOfl HCCJiejíOBaHHJI daKTepHMHg, HO KOTOpOMy 3HaHHTeJIL-HO COKpaTHTCH BpeMH H HyTB C MOMeHTa B3HTHH KpOBH H3 B6HH £0Β03ΛΘΗ0ΤΒΗΗ 66 Ha SHJÍKHe H TBepffiHe ΠΗΤΗΤΘΛΒΗΗβ ΟρθΑΗ C ΚρΟΒΒίΟ,h co3^aHHe TaHoro odopynoBaHHH, κοτοροβ dyneT coneTaTB Bce He-OdXOJtHMHe gyHKHHH GHCTeMH B3HTHH KpOBH H CHCTeM jyra reMOKyjiBTy-pa b npesejiLHO κομπηκτηοη KOHCTpyKiOT, cooTBeTCTByioiueS Ha3Ha-ΗΘΗΗΚ). B Η3θΰρθΤΘΗΗΗ ΜβΤΟβ ΒΗΗΒΤΓΘΗΗΗ daKTepHMHfi, KOBJÍH OTCaCHBaeTCHH3 ΒΘΗΗ B EO3HpOBaHHOM KQJIHHeOTBe ΚρΟΒΒ Η Ηθρβ3 CHCTeMy B3HTHHκροΒΗ nonajiaeT b cocyu chct6MH atih reMOKyjiBTypH, HaxoAWfficn .πολ BasyyMOM h/htih HanojnieHHHH οπθιζηηαβηημ ra30M η npn 3 tomCMemHBaeTCH b khakoh nHTaTeJiBHOH cpe^ofi, xapaKTepH3yeTCH τβΜ,hto κροΒΒ, oTCocaHHaH b cocyu, oAHOBpeweHHo HHOKyjiHpyeT shskpoh CMeiiiajíHyio c HeS TBepayio πητητθλβηυχι cpeAy. Upn onpeAejreHHofíTewnepaType η ηηκεοηηομ ποαοκθηηη cocysa οηοτθμη &amp;jlh reMOKyjiB-Typaa b odenx πητητθαβηηχ cpe^ax BHpadaTHEaeTcn nocjie θτογοKyjiLTypa daKTepuS h CTaHOBHTcn bhehmoh djraroAapn HHAHKaTopy—KpacHTejixi, conepsameMyca b πητβτθαβηοη cpeae.
CooTBeTCTByiomee H30dpeTeHHKi odopynoBaHHe Xjlh βηηβαθηηη daKTe-pHMHg, COCTOfDHee H3 CHCTeMH B3HTHH KpOBH Η CHCTeMH RKR ΓβΜΟ-KyjiBTypa, xapaKTepn3yeTCH τθμ, ήτο cocyu οηοτθμη ajih reMOKyjiB-Typa npeflCTaBJraeT codofi ai-myjiy c KOJiBneodpa3HHM · cyseHiíeM h htonoJio&amp;eHne θτογο KOJimeodpa3Hóro cyjxeHHH.no jvruHe ai.myjm npn- cnocoÓJieHO κ TpeóyioiueMycjť οοοτηοπιθηηκ) κοληηθοτβη τβθραο2 η -OTAKOÍ1 ΠΗΤΗΤΌΛΒΗΟΗ ΌρθΑΗ» ΠρθΙΠ.ψΙΙΙΘΟΤΒΘΗΗΟ 1:3. Jíajiee, oÓopýaoBáHne, cooTBeTCTByiomee Η3θόρβτβΗΗκ, xapaKTepiray-θτοη τβΜ, ήτο b cocyAe οηοτθμη ajih reMOKyjiLTypH scHAKaH h TBep—Aan imTaTejiLHaH cpe.ua pacnaHOJKeHH noonepeAHo η πρπ ποαοκθηηη' cocy.ua b AHana30He HaimoHa O -135° conpHKacajOTcn Apyr c ApyTOM,h ήτο odteM η. πατιο&amp;θΗΗθ TBepAoS BUTaTejiLHOH cpejxH (ShkcnpyeTcaKOJimeo(5pa3HHM cyzeHHeM. y K0Jrmeoópa3Horo cyxeHHír Ha noBepx-hocth HMeeTCH nepMaHeHTHoe kojibiío, no3BOJinoisee npoH3BéCTHrjia£Koe οταθαθηηθ odenx ήηοτθη cocyna ahh reMOKyABTypa de3 oc-kojikob. JínaweTp nponycKHoro OTBepcTHH KOJimeodpa3Horo cysceHásHaxoflHTCfl b npe;n;ejrax 6^ 10 mm, t.k. κοληήθοτβο h noJioxteHnearapoBoií $a3H 3aAaHO Hanajiow pannyca cysceHHH οθήθηηη. OdopyAO-BaHse, cooTBeTCTBywe H3odpeTeHino, xapaKTepireyeTCH eine η τβΜ,ήτο cocyA CHCTeMH jyin reMOKyABTypH coeAHHeH c cncTeMofí b3hthhKpOBH OAáCTKHHOH TpydOHKOH ΠρθϋΜ^ΘΟΤΒθΗΗΟ H3 ΜΗΓΚΟΗ IUiaCTMaC-ch h npeácTaBJíaeT codofi 3βκρπτγιο ποοαθαοβ3τθαβηο pacnoJioseHHyKiHaCTL, H OCB CHCTeMH B3HTHH KpOBH paCHOJIOKeHa ΠΟΑ yrJIOM K OCHοΗοτβΜΗ ααη reMOKyjiBTypH h ee moshó ποβορ&amp;ήηβητβ b jnodoM HanpaB·ΑΘΗΗΗ.
CooTBeTCTByHqas H3odpe Temno cncTeMa b3hthh κροΒΗ xapaKTepirayeT-CH ΤβΜ, ΉΤΟ HHSHHH HaCTB TpydOHKH HHBeKUHOHHOfi ΗΓΑΗ yjyiHHeHa.TaK, ήτο TpydoHKa oxBaTHBaeT odJiaMHBaeMHH οτθκτιηηηηη κοηθπ co-cysa οηοτθμη ajih reMOKyABTypH, djiaroAapn newy odJiai.nmaTB οτθκ-JLHHHHH KOHeiX MOSHO ΘΠίθ £0 B3HTHH KpOBH. ΠΟΜΗΜΟ ΤΟΓΟ, odjiaMHBaeMHH ΚΟΗθΠ CHCTeMH Β3ΗΤΗΗ KpOBH, SAaCTHH-Han TpydoHKa π οηοτθμη b3hthh κροΒΗ OKpyseHH npiweraioinHM kojt-ΠβΉΚΟΜ AJIH 33ΰξΚΤΗ ΟΗΟΤΘΜΗ Β3ΉΤΗΗ KpOBH. ITpHMep ocymecTBJíeHHg H3odpeTeHHg JIoctohhctbo MeTOAa h oóopyAOBaHHH, cooTBeTCTByioiiíera H3odpeTe-HH30, 3aKTK>HaeTCH B TOM, ΉΤ0 djrarOAapn Κ0Μ0ΗΗΗρ0ΒΗΗΗΕ> MeŤO£a HOdopyAOBaHHH CHCTeMH B3HTHH KPOBH H CHCTeMH A&amp;H reívlOKyJILTypHco3AaeTcn odBeAHHeHHé κθοκολγβκηχ ao ότογο pa3po3fíeHHHX· 9TanoBπρπ ΟΑΗΟΒρβΜβΗΗΟΜ ΚΗΉβΟΤΒβΗΗΟΜ yJiyHUieHHH MeTOAa B3HTHH H aHa- , JIK3a KpOBH C nOMOIUBIO CBOÓOflHOro OT 3βΓρΗ3ΗβΗΗΗ H yftOÓHOrO BnOJIL3OBaHHH OÓOpyftOBaHHH. ΠθΜΗΜΟ ΤΟΓΟ, dJiaTO^apH ΠρΗΜΘΗβΗΗΚ)BUcoKOKaHečTBeHffflX nuTaTeJíLHiix cpefl c aHTHdaKTepHniWHLiMH #o-óaBKawH h TBepnnx γοπήτθλβηηχ cpe# c HH&amp;HKaTopaMH—κρβοητθ&amp;ημηCQ3flaeTCfl B03M0KH0CTB B3HTHH ΜθΗΒΙΗθΓΟ ΚΟΛΗΗΘΟΤΒΗ KpOBH H HHČĎH—iXHpoBaHiiá ee KyjiBTypawH daKTepHŠ. B οοοτβθτοτβηη c coctsbom ifflTaTeJiBHoS cpe^H, coftepEamefi θτηonpejieJieHHHe aodaBKH, mojkho BupamnBaTB tojibko HedoJiBiuoe kojih-ΗΘΟΤΒΟ MHKpOOpraHH3MOB,. Τ.Θ. dpaTL ΜΘΗΒΠΙθβ KOJIIFíeGTBO KpOBH H-odieM odenx naoTeS cocyna chotomh &amp;eh rewoKyjiBTypH coxpaHHTLΜβΗΒΠΙΗΜ, ΗβΜ y Η3ΒΘ0ΤΗΗΧ yjKe COCyHOB ftJIH reMOKyJIBTyp.
Diaro^apH ojmoBpeMeHHofi ηηοκυληιϊηη jkhukoh η TBepnoS πιίτητθηβ-hoh cpeš, a Tarase ÓJiarosapa onTiraecKOMy npeftCTaBnem) pa3MH0-ΣίβΗΐΐΗ daKTepnĚ odecneHBBaeTCH 3Ηηήητθ^ιβηοθ ynpomeHHe BceroweTQfla, a TaKse coKpameHHe Η3Αβρκβκ Ha patíoTH no daKTepiiojiorHH.HenocpeflCTBeHHaa nojjana κροΒΗ b XByx$a3HHS cocyn jyin reMOKyjiL-TypH, dHCTpaH HHOKyjiHHHH odenx rorraTejiLirax cpe# nocjie b3hthhκροΒΗ h HHKydaiTO KyjiBTyp' daKTepuS b AByx ήηοτηχ cocysa jyiHreMOKyjiBTypH de3 nocneftyioinero nepeHeceHEH h noTowy de3 3arpH3-ΗβΗΗΗ Π03Β0ωΐΗ3Τ ΠρΟΒΘΟΤΗ dHCTpHH Η Η8£β3ΚΗΚ&amp; #ΗΗΓΗΟ3.
OdBejumeHHe jkhhkoé η τβθρηοη ΠΗτατβΛΒΗοδ cpea b cooTBeTCTByio—meM H3otípeTeHEio cocyse jwlh reMOKyjiLTypa no3BOHHeT jyin ycKope-hhh pocTa daKTepnS y onpe^ejreHHHx kojtohuS opomaTL tíazree iizmMeHee noBepxHocTB TBep®o0 arapoBOH $a3H nepe3 onpeAeHefíHHe npo-KiesyTKH βρομθηη tohkhm cjioeM khukoh imraTeJiiHofi ope^n npn Han-jiohhom noHoaceHHH cocyHa.
DiarosapH MeTojiy, npejyiaraeMOMy κβοόρβτβΗΗβΜ, μοκθτ He ποτρβ-doBaTBca OTflejii.Hoe cydByjiBTHBHpoBaHHe H3 cocyna c reMOKyHLTy-pofi η τθμ caťMM He noTpedyioTCH TexHHKO-TexHOTiornHecKHe 3aTpa-th. KjiarojiapH KOJH»ueodpa3HpMy cysemro mojkho nocne pa3MHO»eHHHdaKTepnS Jierno pa3flejiHTL jjeTaziH cocy^a c rewoKyziLTypoH λζιη .Toro, HTodu HccjiesoBaTL ode ΠΗΤΒτβΠΒΗΗβ cpejni owjibho Apyrστ jípyra. Enaro^apn Kozimy Kazrmeodpa3Horo cyseHHH mojkho προ- ·HŠBecTH iuiajíKHíí pa3jroM no hhhhh, He noBpejíHBmiicL npu stom. - 9 - i ‘ · * . ' ' ·
Ha HHaecTOHmeM npHMěpe- πρηβΟλητοη Ή ο0έηοηηθτοη μθτοβ η odopy-AOBamie, npejvraraeivMe η300ρθτθηηθμ. ΠρΗΜβρ ηηγλή^ηο ποηοηηθτοηHa npnnaraeMOM nepTese; Ha ηθμ npe^cTasjíeHo: ΦΗΓ. CxeMHTHHeCKOe ΠρβΑΟΤΘΒΛβΗΗθ ΠρΟβΟΛΒΗΟΓΟ οθηθηηηΜΟΑ&amp;ΚΗ, npe^iaraeMOH'Η3θύρθΤΘΗΗΘΜ.
CorJiaCHO ΜΘΤΟΑΥ HCCJieflOBaHHH daKTepHMHS .B ΚρΟΒΗ Η3 BQHH naiXH-θΗΤΗ B £03Hp0BaHH0M ΚΟΤΙΗΗΘΟΤΒΘ Ήθρβ3 CHCTeMy B3HTHH KpOBH, CHC-Téwy HTJI Η7ΠΪ mnpimOB ΟΠρθΑΘΛΘΗΗΟΗ KOHCTpyKmiH OTCaCHBaeTCHκροΒΒ λπη aHajiH3a b ctokjihhhhS cocynATiH reMOKyJTLTypH noa Bany-yMOM JWH 3anOJIHeHHHH ra30M H 3βΤβΜ CMeiIIHBaeTCH C W^KOÍÍ ΠΗΤβ-TeJiLHoS cpejioíi, coflepKameĚCH b cocyne. OTcocaHHan τβκΗΜ odpa3OM ' · κροΒΒ HHOKyjmpyeT jnwKyio cpeřcy c aHTHdaKTepwnXHUMii λο0ηβκημη, / ΠρΗΗΘΜ ΟΛΗΟΒρθΜΘΗΗΟ CMeCBJO KpOBH H ΠΗΤΗΤβΛΒΗΟΗ CpeflH HHOKyJIHpy- eTCH TBepnan ΠΗτβτβΛΒΗθΗ cpena b cocyne c reMOKyn&amp;TypoS. IlocjreθτοΓο npn onpeaejieHHofí TeMnepaType h ΗβκηοΗΗΟΜ nonoaeHHH cocy-sa, b eaBECHMOCTH ot ncanenyeMHx KyjiLTyp daKTepuS b odenx HHTa-τθλβηηχ cpenax BupadaTHBaeTcn daKTepnajiBHan Kym&amp;Typa. BnaronapníodaBJíeHHfo HHjwaTopa-KpacHřejin npeHMymecTBeHHO κ TBepnoS nzTa—TejiBHOĚ cpene npenocTa&amp;nneTcn bo3mohhoctb odHapyzeHnn daKTepnS,pa3MHOKHBIUHXCH B ΟόθΗΧ ΟρββθΧ.
OdopynoBanne, npejyraraeMoe ΗβοόρβτβΗΗβΜ, coctoht H3 οηοτθμηΒ3ΗΤΚΗ KpOBH Η ΟΗΟΤβΜΗ Mff reMOKyJIBTypíí. ΟΗΟΤβΜΗ JVIH ΓβΜΟΚγΛΒ-TypH coctoht H3 οτθκΕΗΗΗΟΓο Kopnyca H3 SByx nacTeS, cocyna JVIHreMOKyjiiTypa Γη 2, npescTasjuromero codoS awnyjiy, cocTomnyi) H3nacTH I h nacTH. 2. 7 ©τογο cocyna I h 2 ημθθτοη KQJimeoópa3HoecyseHHe 3, pa3nejraionjee cocyn B&amp;nHHy b οπρβββΛβΗΗΟΜΟΤΗόπιβΗΗΗno od-Beiy 1:3.
Ha noBepxHocTH KCwrmeodpa3Horo cysefínn 3 no odBéwy pacnonoseHOneprvíafíeETHoe Koňmo 9, odecnenHBajoiqee, ánaronapn BHyTpeHHewyHanpnseEHE) b cTemre, de3onacHoe, ήηοτοθ h npocToe pasnejienneodenx nacTeS cocyna c retóóicynBTypoá I h 2. JlnaLieTp KOJimeod-pa3Horo cysemin 3 3θβηοητ ot nzaMeTpa nponycKHoro οτΒβροτηηjyin npnEHBOHHoS ne τjih h paBeH npndJi. 6 10 mm. ' _
. ΕπΕΚβΗ e TBepnan ΠΗτβτοηΒΗΗβ ~ cpenn pacnonoKeHH b cocyne jyin'reMOKyjE&amp;TypH I; 2 ηοοηβηοΒΗτοηΒΗΟ h conpzKacaioTCH npyr c npy-γομ τθμ, hto B nacTH cocyna jyra reMOKyjiBTypn I; 2 conepsHTCH τΒθρηπϋ arap, a b nacTH I - JKHEKaa γηπήτθλβηοη cpena II. Ejia-ronapn KOJn>neoópa3HOMy cy^emno 3 φπκοΗργθτοη odfcéM h noJiose-HHe TBepnoro arapa τθμ, ήτο oh ysepsumaeTca η3γη6ομ cyzeHiiH.OdseM, a τθμ cai.MM h pa3wep cocysa jvih reMOKyjiLTypa I; 2BapLHpyiOTCH B ΟΟΟΤΒΘΤΟΤΒΗΗ O B3HMaeMHM KQTIHHGCTBOM KpOBH. EJia-rcináps co^eTarano HHTaTeJiLHHX cpen c οπρθαθλθηηημη aHTHdaKTepn-UHAHHMH flOdaBKaMH ΠΟ ΟΤΗΟΙϋβΗΗΚ) K SaiUHTHHM ΒΘΙΠΘ0ΤΒ3Μ TeJiaCOrJiaCHO Η300ρθΤΘΗΗΚ) CO3JíaeTCH B03M0JSH0CTB dpaTB jpifl aHaoBaMeHLmee κοληήθοτβο kpobhh τθμ οθμημ coxpaHHTB waraS pa3Mep hoóbgm cocynoB I h 2, t.k. y&amp;e HedcuiBinoe κοπηήθοτβο . Mimpoopra-hh3mob jvih BHpamHBaHHa b reMOKyjiBType naeT 3aMeTHy0 peaKióao.
Cocya jyin reMOKyjiLTypH I; 2 npeuMyiuecTBeHHO npencTasjínoT co-dofí aivmyjiy c odJiaMHBaeMofi οτθκλήηηοη BepxyuiKofi 4, oKpyseHHoS ' .azracTiraHHM οοθληηητθλβηημ MaTepnajroM 5, Hanp. TpydoHKoS H3npo3paHHofí xwacTMacoH mm ΠΒΧ Tuna“60 s kL«.r- ” t3khm odpa3OM cwc-Tewa &amp;jlh reMOKyjiLTypu οοθηηηηθτοη c cHCTewofi b3hthh kpobh hnpn nocjienoBaTeJiLHOM pacnoJioKeraiH npencTaBJUieT codoS θληηοθ .nejioe.
Diaronapn sJíacTHHHOH οοθαηηητθλβηοη TpydonKe 5 ocb οηοτθμηb3hthh κροΒΗ pacnojraraeTcn no# yrzioM κ och οηοτθμη jmih γθμο-KyjiLTypH h ee mosho ποβορ&amp;ηηβθτβ b jtoóom HanpaBJíeHHH. CaoTeMaB3HTHH KpOBH COCTOHT H3 ΕΗΒΘΚϋΗΟΗΗΟΗ HTJIH 6, 3aKJHOHeRHOS B .CT.eKJWHHyu odúJioHKy7H ynJLHHeHHyio BHH3y tsk, hto što yzyiHHeHHeoxBaTHBaeT odJiaMHBaeMyio Bepxyumy 4 cocyna jpra reMOKyjn>TypHI; 2. OdJiaMHBaeMaH BepxymKa 4* cocyna jyra reMOKyjiBTypH I; 2,sJíacTHHHaji coeřCHHHreJiBHaH TpydoHKa 5 h οηοτθμη B3hthh kpobhoKpyjsenH κοππηηκομ 8.
IIpocTpaHCTBO 12 HaxofíHTcn non BaicyyMOM hhh BHpainHBaHHHcnenHaJE&amp;HHx KyjiBTyp daKTepnS 3ηπολήθήο ra30M c onpeneneHHHMnasjíeHHeM. JUh npoKajiHBaHHH βθηη nepen πολβ3οβ3ηηθμ o. npHcno—codjieHHH cHHMaeTCH KOJinanoK 8, οτθκιιηηηθη odoJioHKa 7 htjih 6Ha£pe3aeTca h odjraíMBaeTcn tsk, hto ooBodoamaeTcn CTepiuiLHanin?jra 6. Ilocjie BKajncBaHHS b-Befly npoH3BQHHT.CH cpesaimnee λβπ&amp;θ-ΗΗΘ ΟΗΟΤΘΜΗ ftTLH reMOKyJIBTypH BOKpyr OCH; ΟΗΟΤΘΜΗ B3HTHH κροΒΗ,noica cTeKTuamaa Bepxynma^He odJioMaeTca; zrera 6 οοτηθτοη ηθπολ-ehshoS. Ποβ B03fleScTBHeM BaxyyMa b HTJie 6 co3naeTcn ηοηηκθη-ΗΟΘ ΒΘΒΠΘΗΗΘ, Tas ΗΤΟ B ΟΟΟΤΒΘΤΟΤΒΗΗ O ΗΜΘΕϋίΗΜΟΗ BaKyyMOM l - II - onpejieJieHHoe kojihhcctbo kpobh aBTOMáTinecKH oTcacHBaeTcn bcocyn I; 2 οηοτθμη bshthh kpobh. ΟβΗΟΒρβΜθΗΗΟ C THKHM OTČaCHBaHHÓM KpOBH B fl03Hp0BaHH.0M KOJIH-ηθοτβθ προποχοβΗΤ BHOKyjumHíi khhko0 cpe^H II. h CMemaHHoS. c HeSTBepnoĚ arapoBoň $a3H 10. - ' Ποαπό yjjajieHHH ηηβθκηηοηηοη htjih 6 H3 βθηη η 33Κρητηη ee οτθ-pmitHOH προόκοδ, npeuMyiqecTBeHHo H3. miacTMáccH, προΒΟβΗτοη πρπonpejiejieHHOM ηθιμοηηομ ποεοχθηηη βοθ® οηοτθμη rjix reMOKyjiLTypnHHKyóamiH miTaTeJiLHoS cpejtH 10 η II npn TeMnepaType, npero.iy-mecTBeHHo b íEana3Ofíe 35-37° C.
OnpefleJieHHoe ηηκτοηηοθ ποτιοκθηηθ cocyna I; 2 οηοτθμη λλη rewo-
- KyjiBTypa TpeóyeTca b 33Βηοημοοτη ot odtewa cocyna, a τθμ čaMHMη ΤΒΘρβοιι mm KiiflKOH πητθτθλβηοΗ cpenn 10; II nm τογο, HTodH ' iuieHKa keekoE rmTaTexbHoa cpenn II ηθοτηηηο noKpHBana noBepx-hoctb TBepnoro arapa.IO. B CBH3H c HHKydaimeíi ιηπήτθλβηοη cpesu ΙΟ; II προΗΟχοβΗΤ pa3-μηοηθηηθ daKTepná, ροοτ Ky&amp;LTyp daKTepirii b odezx cpenax cTa-HOBHTCH SaMeTHHM, COímaCHO H30dpeTGHHH), ΠΟ Η3ΜΘΗΘΗΗΚ) ΙΧΒΘΤΘΟ,ζΗΟΓΟ H3 3ΤΗΧ flOdaBJIGHHHX HHAHKaTOpOB.
IvtopodHOjrormiecKoe HccJiesoBajme προΒοβΗτοη οτβ&amp;ΖΓΒΗΟ nyTeM pa3-£ΘΛΘΗΗΗ ΟΗΟΤΘΜΗ B3HTHS KpOBH H ΟΗΟΤΘΜΗ C reMOKyJlLTypOÉ HOCJieHanpe3aHEH h oTJiaMHBaHHH nacTH I h 2 cocyna I; 2 y KOJima 9cyseimn 3. ' B CBH3H C 9THM ΠρΟΗΟΧΟβΗΤ ΟΤΛΘΛΘΗΗΘ KH£KOH θρθ£Η II OT ΤΒΘ£«ΟΪΙ ,cpenn, odpocmefí daKTepHHMH 10, τηκ ήτο οτθηοβητοη βοθμοηηημpa3seJii)HHfi saceB η β3Ητηθ aHannsoB sjih naHBHeSnieH.daKTepHOJio-rmiecKoS odpadoTKH mm HccjresoBaHEH odenx cpefl. ΟτββΛβΗΗβ jkhhkoS ηητθτθλβηοη cpenn II ot TBepnofi arapoBoii $a-3H 10 npoE3EojuiTcn πρΗ οτλομθ cocyna I; 2 οηοτθμη B3sthh κρο-bh TaK, hto nacTL 2 c arapoM Hanpamiena BBepx. Bjiaronapn θτο-My cocyn I; 2 mo&amp;ho. 3anojiHHTB onpeneJieHHHM ra30M, b κοτοροΜMoryT pa3MHosaTLCH daKTepra, TpynHo odHapysusaeRiHe b κποΛοροη-ho0 aTMocčepe.
Φο pMyjra - H3 od ροτθηηη 1. MeTOfl ΒΗΗΒΛβΗΗίϊ tíaitTepHMHK, npž KOTOPOM KPOBB OTCaCHBaeTCHB AO3HpOBaHHOM KOJBWeCTBě ΗΘρΘ3 CH0T6My B3HTHH KpOBH B CO-cyA πόα BaicyyMOM h/oh sanoJiHeHHHS cneiwr&amp;HHM ’ra30M jyiflreMOKyuiBTyp η npn : stom cMenumaeTcn c wckoíí πητητθλβηοΑcpeftoS, oTjnnanimHCH τθμ, hto kpobb, oTcocaHiian b cocys ;oAHOBpeMeHHo HHOKyjiHpyeí wtKyio nnraTeJiBHyip cpejiy caHTH-.daKTepniTOHHi^iH AOóaBKaMH η, ομθπιθβπιποβ c HeS, - TBepnyu πη-TaTěJiLHyio cpeAy, η τθμ, ψγο ποοαθ βτογο πρκ onpeAeJieHHoSTeMnepaType h HamioHHOM ποαοηθηηη cocyaa b odenx cpeaax bh-padaTHBaeTcn KyjiLTypa daiíTepnS η οτηηοβητοη 38Μθτηο3 dJiaro-Aapn AodaBKe HHOTKaTopa-KpacHTeAff. 2. OdopynoBaHne jvih ηοοηθαοβηηηη tíaKTepHMH0, cocTonmee H3 chc-ΤΘΜΗ B3HTHH KpOBH, H3 ΗΗΒΘΚΙΧΗΟΗΗΟΗ UTAH CO CTeKJTHHHOS OdO-JIOHKOS H OTA6JIBHO0, CBOdOAHO ΟΟΘ£ΗΗΗΚ)ΐηΘΗΟίϊ Ο,ΟΗΟΤΘΜΟβ B3H-THH- KPOBH, ΟΗΟΤΘΜΗ ÍVW reMOKyJILTypH, COCTOHIUeS H3 3anaffH-Horo cocy^a c khakok HHTaTeji&amp;Hot cpeaoS, oTjnreaioineecn τθμ,hto sanaHHHHfi cocys οηοτθμη ahh reMOKyjiLTypn (I; 2) npeA-cTaBJíHéT codoS aimyjiy c K0Jimeodpa3HHM cysemieM (3), a Hase .KQ3imeodpa3Horo cy^eHHs (3),stom $MKCHpoEaHo/fíáxoAHTCH TBepnasnHTaTeJiLHas cpeaa (10) h BHme Koini»neodpa3Horo cysenus (3)HaxoAHTCH wCKan nnraTejiLHaH cpe^a (II), β κολήήθοτβθηηομοοοτποπιθηπη paBHOM npeHMyigecTBeHHO 1:3. 3. OdopyAOBaHne no n. 2, oTJuraammeecH τθμ, hto Ha ποβθρχηοοτηKOdn>neodpa3HoiO cysemoi (3) ημθθτοη kohbiío λομκη (9), hAnaMeTp ero nponycKHoro οτβθροτηη οοοτθβ^ιηθτ 6 — 10 mm, h ero πολοκθηηθ baojib jvihhh ai.myjm CoóTBeTCTByeT κοεηηθοτ-BeHHowy οτηοιιιθηιπο ŤBepnofi ΠΗΤβΤΘΑΒΗοβ čpeAH κ swoS (.10; II). 4. OdopysoBaHne no n.2, oTJnrcaionieecH τθμ, ητο cocya οηοτθμηPJifi ΓΘΜΟΚΥΛΒΤΥΡΗ ΟΟΘβΙΪΗΘΗ C CIICTeMOfi E3HTI1H KpOBH ΉθρΘ3anacTUHflyio TpydoHry (οοθαηηητθλβη^. ήηοτβ) (5) , npeiiMy-mecTEeHHo H3 npo3paHHoíí mhtkoh mracTtiaccH, κθκ 3aKpHToe / B 60 ΠΟΗΟΚΘΉΗΙΙ ποωίθίζΟΒΗΤ&amp;πΒΗοβ eflHHoě neaoe, η ocl οηοτθμη b3htjw κροεζpacncvrojKena πολ yrJioM κ ocii οηοτθμη jym reMOKyjrLTypn η ee'ΜΟ3ΚΗΟ nOBOpa^HBáTL B JWÓOM H3JipaBJI6HKK. . . 5» OdopynoBamie no n. 2, oTJinnaroineeca τθγλ, hto odiaMHBaeMHfi- οτθκζΐΗΗΗΗΐΙ κοΗθη (4) cocy^a οηοτθΜΗ &amp;tih reMOKyjr&amp;TypH (I; 2) pacnouio&amp;eH b hjdkhoS nacTH eTeKJLHHHofi Tpydomcz (7),oxBaneHHoS oJiacTiraíoS οοθληηητθλβηοζ TpyÓKofi (5). 6. OdopysoBamie no n.. 2, oTjnraawecfl τθμ, hto. saacTiniHaa .coeAHHHTeJiLHaH nacTL (5), KHBeKnnoHHaa mma (6) z οτθκίηη-Haa TpydoHKa (7) HazpuTH κολπβηκομ (8).
AHHQTaw
IfeoópeTeHHe KacaeTcn μθτολη h odopynoBaHna jym βηηβηθηηη tíaK-ΤβρΗΜΗβ nyTeM ΠρΗΜΟΓΟ B3HTHH KpOBH H KyJIBTHBHpOBaHHH reMOKyJIB-Typ. . · .
IfejIB» Η3Ο0ρθΤβΗΗΗ ΗΒΛΗΘΤΟΗ ynpoqeHHe ΜΘΤΟ£ά Β3ΗΤΗΗ KpOBH Η. reMOKyjiBTyp η υμθηβπιθιιηθ 3aTpaT Ha annapaŤypy. Β OCHGBy Κ3Ο($ρθΤΘΗΙΙΗ HOJTOJKeHa sahana 003Λ3ΤΒ μθτο#, πο κοτορο- . My coKpainaioTOH Bpew h nyTB,. nannHan c μομθητα ββητηη κροΒΗ soB03£efíCTBHH Ha 3ΚΗ£ΚΗΘ H TBepUHe HHTaTeJILHHe KyJlBTypH C KpOBBDh co3flaHHH oóopyáoBáHiíff c ΜβΗΒΐιπίΜ kooHecTBOM neTajien. KpOBB, ' OTCOCaHHaa B ,HO3HpOBaHH0M ΚΟΛΗΗΘΟΤΒΘ CHCTeMOS B3HTHH KpOBH Bcocy#,- HHOKyjiHpyeT οεηοβρθμθηηο aujncyio h TBepnyio miTaTejiBHyBcpeny h OTHMyjrHpyeT b hhx KyjiBTypy daKTepnít, KOTopan cTaHOBHTcabhahoh djiaro^apn HHAHKaTopy-KpacHTejno. y CHCTeMH flJLH reMOKýJIBTypH ΗΜΘ6Τ0Η COCyH B BW aMnyjm G KOJIB-rteodpa3HHM cyseHHeM. ÍIojroseHHe K0JiBixeodpa3Horo cyjKeHHH npncno-co&amp;ieHo no jyiHHe aMnyjm κ HeodxoaHMOMy οοοτηοπιθηκη) KQjnraecTBaTBépnoS H jkhjucoS nHTaTejiBHOH cpenu, npeHMymecTBeHHO κ oŤHorne-hhh) 1:3. Ode nHTaTeJiBfíHe cpe#n pacnoJioHeHH nocjreflOBaTejiBHO h-3a$HKCHpOBaHH Β ΟΒΟΘΜ HOJIOSeHHH.
Ha φπτ. X npe^cTaBJíeHo. odopynoBamie, npe&amp;JiaraeMoe Η3θόρβτβΗΗθΜ.

Claims (6)

9 Předmět vynálezu
1. Způsob zjišťování bakterií, při kterém se krev odsáváve stanoveném množství systémem pro odběr krve do nádobkypod vakuem a/nebo naplněné speciálním plynem pro hemokul-turu a při tom se mísí s kapalným živným mediem, vyznačující setím, že krev odsávaná do nádobky současně inokuluje kapalnéživné medium s antibaktericidními přídavnými látkami a přisměšování a ním pevné živné medium a pak se při určité teplotěa naklonění nádobky kultivuje v obou mediích kultura bakterií,která se stává viditelnou přídavkem baryiva-indikátoru.
2. Zařízení pro zjišťování bakterií sestávající ze systémuodběru krve, z injekční jehly se skleněným obalem a odděle-ného, volně se systémem odběru krve spojeného systému prohemokulturu, tvořeného zatavenou nádobkou z kapalným živnýmmediem, vyznačující se tím, že zatavenou nádobkou systému prohemokulturu je ampule s kruhovým zúžením (3) a pod kruhovýmzúžením (5) je fiksováno pevné živné medium (10) a nad zúžením(3) se nachází kapalné živné medium (11), v poměru množství 1,5
.3· Zařízení podle bodu 2, vyznačující se tím, že na povrchukruhového zúžení (3) je kroužek (9) lomu a průměr jehoprostupního otvoru činí 6 - 10 mm a jeho umístění na délceampule odpovídá poměru množství pevného živného mediá (10)ke kapalnému živnému mediu (11).
4. Zařízení podle bodu 2, vyznačující se tím, že nádobkasystému pro homokulturu je sp'o je na se systémem odběru krveelastickou částí (5) z průhledného měkkého materálu z plasti-cké hmoty, jako uzavřené za sebou následující jednotné tělesoa osa systému odběru krve je umístěna pod úhlem k ose systémupro hemokulturu a je možno ji otáčet v libovolném směru·
5. Zařízení podle bodu 2, vyznačující se tím, že olemovanáskleněná špička (4) nádobky systému pro hemokulturu je umístěnave spodní části skleněné trubičky (7)» obklopené elastickouspojovací částí (5)·
6· Zařízení podle bodu 2, vyznačující se tím, že elastickáspojovací část (5), injekční jehla (6) a skleněná trubička (7)jsou zakryty krytem (8) Uznáno vynálezem na základě výsledku expertizy, provedenéÚřadem pro vynalezectví a patentnictví, Berlín, DD 1 výkres
CS376180A 1979-06-28 1980-05-28 Method of detection of bacteria and equipment to perform this method CS230958B1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DD21396079A DD149843A3 (de) 1979-06-28 1979-06-28 Verfahren und vorrichtung zum nachweis von bakteriaemien

Publications (1)

Publication Number Publication Date
CS230958B1 true CS230958B1 (en) 1984-09-17

Family

ID=5518952

Family Applications (1)

Application Number Title Priority Date Filing Date
CS376180A CS230958B1 (en) 1979-06-28 1980-05-28 Method of detection of bacteria and equipment to perform this method

Country Status (11)

Country Link
AT (1) AT366713B (cs)
BG (1) BG34473A1 (cs)
CH (1) CH652295A5 (cs)
CS (1) CS230958B1 (cs)
DD (1) DD149843A3 (cs)
ES (1) ES492885A0 (cs)
FI (1) FI70045C (cs)
FR (1) FR2460332A1 (cs)
GB (1) GB2054144B (cs)
SE (1) SE448737B (cs)
SU (1) SU1440925A1 (cs)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE584241A (cs) * 1958-11-04
US3671399A (en) * 1968-08-30 1972-06-20 Hoffmann La Roche Polyanionic compounds in culture media
US3692486A (en) * 1971-07-12 1972-09-19 Cybertek Inc Methods and apparatus for obtaining the quantitation and the concentrations of precipitin reactions and participating molecules in biological fluids
US3817240A (en) * 1972-06-28 1974-06-18 Becton Dickinson Co Multiple sample needle assembly with one-way valve and blood flow indicator
US4026767A (en) * 1975-11-19 1977-05-31 Wisconsin Alumni Research Foundation Test procedure for microorganisms in blood
GB1601689A (en) * 1977-07-15 1981-11-04 Mariel C Method of diagnosing bacteremia and apparatus therefor
SE452336B (sv) * 1978-06-16 1987-11-23 Boehringer Mannheim Gmbh Forfarande for pavisning av sjukdomsalstrande mikroorganismer vilka adsorberats extrakorporalt pa en selektivt bindande adsorbent

Also Published As

Publication number Publication date
SE448737B (sv) 1987-03-16
SU1440925A1 (ru) 1988-11-30
GB2054144A (en) 1981-02-11
FR2460332A1 (fr) 1981-01-23
FI70045B (fi) 1986-01-31
DD149843A3 (de) 1981-08-05
ES8105928A1 (es) 1981-05-16
CH652295A5 (de) 1985-11-15
FI801166A (fi) 1980-12-29
FI70045C (fi) 1986-09-12
ATA174680A (de) 1981-09-15
GB2054144B (en) 1984-02-15
FR2460332B1 (cs) 1984-12-07
SE8002556L (sv) 1980-12-29
ES492885A0 (es) 1981-05-16
BG34473A1 (en) 1983-10-15
AT366713B (de) 1982-05-10

Similar Documents

Publication Publication Date Title
US8292869B2 (en) Container for vial of radiopharmaceutical and set for its infusion in a patient or for its transfer elsewhere
ES285095U (es) Jeringa perfeccionada para uso medico.
Merkel et al. Comparison of indium-labeled-leukocyte imaging with sequential technetium-gallium scanning in the diagnosis of low-grade musculoskeletal sepsis. A prospective study.
Miyauchi et al. Relation of doubling time of plasma calcitonin levels to prognosis and recurrence of medullary thyroid carcinoma.
Scheible et al. Percutaneous aspiration of adrenal cysts
Wong et al. Clinical studies on core-carrier obturation: a systematic review and meta-analysis
CS230958B1 (en) Method of detection of bacteria and equipment to perform this method
KR20020033782A (ko) 비경구 투여용의 약학적으로 안정된 옥살리백금 조제품 및그 조제방법
Lewis et al. Radium-223 in bone-metastatic prostate cancer: current data and future prospects
Toller Permeability of cyst walls in vivo: Investigations with radioactive tracers
Curling Observations on cystic disease of the testicle
Hulka et al. Methodological issues in epidemiologic studies of endometrial cancer and exogenous estrogen
Moe et al. Maxillectomy and targeted radionuclide therapy with 153Sm‐EDTMP in a recurrent canine osteosarcoma
Goren et al. Clinical and radiobiologic features of Thorotrast-induced carcinoma of the maxillary sinus: A case report
Bissell et al. Radioimmunoassay of serum albumin produced by human embryonic liver in monolayer culture
Borah et al. Further studies on the prognostic importance of Barr body frequency in human breast cancer: with discussion on its probable mechanism
Das et al. Odontogenic myxoma: A case report and review
NO303426B1 (no) Injeksjonsspr°yte
Nishida et al. Single-dose four hour dexamethasone suppression test in normal men and its application for the diagnosis of Cushing's syndrome
CN110038006A (zh) 表没食子儿茶素没食子酸酯联合酪氨酸激酶抑制剂在制备癌症治疗药物中的应用
Turner-Warwick Intravital Techniques for the Demonstration of Lymphatics: The introduction to an Hunterian Lecture on The Lymphatics of the Breast delivered at the Royal College of Surgeons of England on 28th May, 1957
BUCHER et al. II. PREGNANDIOL AND ESTROGEN OUTPUT IN THE URINE OF PATIENTS WITH CHRONIC CYSTIC MASTITIS
Herbstein et al. Pterygium removal. A technique to prevent recurrence.
Phan et al. Primary osteogenic sarcoma of theurinary bladder successfully treated with combination therapy
Chan et al. Tract sealing with normal saline after percutaneous transthoracic lung biopsies–promising but better evidence required.