CS230958B1 - Method of detection of bacteria and equipment to perform this method - Google Patents

Method of detection of bacteria and equipment to perform this method Download PDF

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CS230958B1
CS230958B1 CS376180A CS376180A CS230958B1 CS 230958 B1 CS230958 B1 CS 230958B1 CS 376180 A CS376180 A CS 376180A CS 376180 A CS376180 A CS 376180A CS 230958 B1 CS230958 B1 CS 230958B1
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nutrient medium
blood
kpobh
container
θηηθ
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CS376180A
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Czech (cs)
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Manfred Kunze
Martin Schambach
Werner Koehler
Klaus Mueller
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Manfred Kunze
Martin Schambach
Werner Koehler
Klaus Mueller
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Publication of CS230958B1 publication Critical patent/CS230958B1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/08—Flask, bottle or test tube
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61J—CONTAINERS SPECIALLY ADAPTED FOR MEDICAL OR PHARMACEUTICAL PURPOSES; DEVICES OR METHODS SPECIALLY ADAPTED FOR BRINGING PHARMACEUTICAL PRODUCTS INTO PARTICULAR PHYSICAL OR ADMINISTERING FORMS; DEVICES FOR ADMINISTERING FOOD OR MEDICINES ORALLY; BABY COMFORTERS; DEVICES FOR RECEIVING SPITTLE
    • A61J1/00—Containers specially adapted for medical or pharmaceutical purposes
    • A61J1/05—Containers specially adapted for medical or pharmaceutical purposes for collecting, storing or administering blood, plasma or medical fluids ; Infusion or perfusion containers
    • A61J1/06—Ampoules or carpules
    • A61J1/065—Rigid ampoules, e.g. glass ampoules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Sustainable Development (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)

Description

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Ho JUiarEOCTEKe óaKTepWtíí E3B©CTHH HeCKOJILKO ΜΘΤΟ^ΟΒ E BEHOBodopysosaHEíi, κοτορκο, ojtfiaxo, óaxee wih Menee oranaioTca ot-tfeJILHHlZZ STanSMH EXE HX BapHaHTaME, a TaXHM OÚpá3OM Η &ΘΤ3ΛΗΜΗoóopysosaHHH. ' Πθ ΠροΟΠΘΚΤΘΜ H3B6CTHa CHCTQMa JUH reMOKýJIBTypH VacutainerB GOOTBeTOTBHH C KOTOpOfí UpOBpflHTCH CJiejiyEHHH© ΟΟΗΟΒΗΗΘ 3TaiHTHccJie,n;oBaHHH óai^Tepiwm: . . - Βθητιιθ κροΒκ: CHCTeMoS B3hthh κροΒΗ H3 βθηη naixneHTa depeTca KPOBB c no-MOUIBIO ΗΓΛΗ, 3aiHHHíeHHOS B ΟΟΟΤΒΘΤΟΤΒΗΗ C DE-OS 2332133, ΟΤΘ-piUILHOrO nuipima HHH Haóopa'&71fl B3HTHH KpOBH H3 ΒΘΗΗ naiíHeH“Ho JUAREOCTEKe óaKTepWtíí E3B © CTHH HeCKOJILKO ΜΘΤΟ ^ BEE BEHOBodopysosaHEíi, κοτορκο, ojtfiaxo, óaxee wih Menee oranaioTca ot-tfeJILHHLZZ STanSMH EXE HX BapHaHTaME, and TaXHM OÚpáMOM Η &;3ΛΗΜΗoóopysosaHHH. H3B6CTHa CHCTQMa JUH reMOKYJIBTypH VacutainerB GOOTBeTOTBHH C KOTOpOfi UpOBpflHTCH CJiejiyEHHH © T 3TaiHTHccJie, nBaHHH aiai ^ Tepiwm:. . CTCTeηι::::::::::: CHCTeMoS 3ρΒΗ3333333333333333333333333333OBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOBOB

Ta. B3HTaH κροΒΒ nonauaeT πρπΜΟ b οτβριυίΒΗΗβ cocya c shhko0 hh-TaTejE&HOH cpeaofi-CHCTeMH jvw peMOKyJiLTypH, KOTopaa npe^cTaB- . ΛΗΘΤ COdoS ΟΤΘΚ7ΙΗΗΗΗΘ TpydOHKH, φΗΘΚΟΗΗ Η7ΓΗ COCyHH pa3JIHH-hhx βΜκοοτβδ, 33κρητηθ pe3HHOBoS npoÓKOfí hjth MeTázunraeCKoSKpauiKoa. 3th βββθοτηηθ cocynH jvlh reMOKyjihTypH 33κρπτΗ οτβ-PhjilhoS προόκοβ; ecjiH npoÓKa ηθ cTepiuiLHas, Torna nepejí B3H- .τηθμ kpobh ee cjie#yeT τιι^τθλβηο odpadoTaTL Hé3HH$HHHpynuHMCpejíCTBOM. lípu BCTaBjreHHH cocy.ua c reMOKyjr&Typofí b κρθιμθηηθ jyia ηγληoncTeMa reMonyjiBTypH coejwaeTca c cHCTeMóÉ b3hthh kpobh.The. B3HTaH κροΒΒ nonauaeT πρπΜΟ b οτβριυίΒΗΗβ cocya c shhko0 hh-TaTejE & HOH cpeaofi-CHCTeMH jvw peMOKyJiLTypH, KOTopaa npe ^ cTaB-. Do COdoS ΟΤΘΚ7ΙΗΗΗΗΘ TpydOHKH, φΗΘΚΟΗΗ Η7ΓΗ COCyHH pa3JIHH-hhx βΜκοοτβδ, 33κρητηθ pe3HHOBoS npoÓKOfí hjth MeTázunraeCKoSKpauiKoa. 3th βββθοτηηθ cocynH jvlh reMOKyjihTypH 33κρπτΗ οτβ-PhjilhoS προόκοβ; ecjiH npoÓKa ηθ cTepiuiLHas, Torna do not pass B3H- .tηθμ kpobh ee cjie # yeT τιι ^ τθλβηο waste T L é é HH3HH $ HHHpynuHMCpejíCTBOM. lime BCTaBjreHHH cocy.ua c reMOKyjr & Typof b κρθιμθηηθ jyia ηγληoncTeMa reMonyjiBTypH coejwaeTca c cHCTeMoÉ b3hthh kpobh.

Ilocjie npoKajiHBaHHH βθηη cocyE ηθ^ημηθτόη Ha οοηοβθηηθ aep&a-τθλη T3K, hto npoóKa npoTHKaeTca η τθμ caMHM noa ΑββοτΒΗθΜBaKyyMa κροΒΒ oTcacHBaeTCH hs βθηη. Πρπ HOHBJíeHHH κροΒΗ ΗΒΗΗΗΗΘΤΟΗ ΗΘΜΘΗΛΓΘΗΗΟΗ HHOKyJIHHHH HHE-koS nHTaTejiBHoS cpejajz. Ecjih noTpetíyioTCH eme aHajiH3H kpobh,το HecKOJiBKO cocynoB jyia rewoKyjiLTypH cjiesyeT noonepesHo3an0JIHHTL pa3JIHHHHMH HHTaTeJIBHHMH ΟρθβθΜΗ. - HosroTOBKa h ηηΦηηηροβηηηθ cydKyjiBTyp:Ilocjie npoKajiHBaHHH cocyE ηθ ^ ημηθτόη Ha οοηοβθηηθ aep & α-τθλη T3K, hto npoóKa npoTHKaeTca η τθμ caMHM noa ΑββοτΒΗθΜBaKyyMa κροΒΒ oTcacHBaeTCH hs βθηη. HOHBJíeHHH κροΒΗ ΗΒΗΗΗΗΘΤΟΗ HO HHOKyJIHHHH HHE-koS nHTaTejiBHoS cpejajz. Ecjih noTpetíyioTCH eme aHajiH3H kpobh, hecKOJiBKO cocynoB jyia rewoKyjiLTypH cjiesyeT noonepesHo3an0JIHHTL pa3JIHHHHMH HHTaTeJIBHHMH ΟρθβθΜΗ. - HosroTOBKa h ηηΦηηηροβηηηθ cydKyjiBType:

Ilocjre topo, Kaz cocyn jyin reMOKyjiLTypH dyneT ynaJieH H3 htjio-JíepKaT&amp;HH Η ΤΘΜ CaMHM ΟΗΟΤΘΜΒ B3HTHH κροΒΗ ΟΤβθΛΗΤΟΗ OT CHC-τθμη λπη reMOKyTiBTypn, a κροΒΒ ηητθηοηβηο nepeMeinaeTCH οsHHKofí miTaTeJiBHofi. cpefloft, ocymecTBHTCH HHKydHpoBaHHecocy-fla c reMOKyjiBTypoS npz 35 - 37° C kok noaroTOBKa κ ηηΦηηηρο-BaHH» cydKyjn>Typ. i -3- ΠρΗ npHCTpofiKe ΗΘ3ΘΚρΗΤ0Γ0 HJIH 38ΚρΗΤ0Γ0 ΒβΗΤΚΤΙΗΐχΗΟΗΗΟΓΟ ΠρΗ“ύποοοάτίθΗΖΗ odecnemiBaeTCH KyjiBTHBHpoBaHHe aapodHHX wih aii-θροόΗΗΧ MHKp00praHH3M0B; B 3aBHCHM0CTH OT HX pOCTa, KOTOpHÉcJie^yeT KOHTpoJiHpoBaTB nepe3 οπρβββΛβΗΗΗθ npoMeayTKH βρθμθηη, . ocymecTBJíHeTCH nocjie ηβηογο πογ.^τηθηηη wkoíí nuTaTejiLHoií cpe-ΛΗ m<)niíiípoBaiBíe cydKyjrBTyp Ha TBepnyio nHTaTejiBHyio cpejiy.Ilocjre topo, Kaz cocyn jyin reMOKyjiLTypH dyneT ynaJieH H3 htjio-JíepKaT & HH ΤΘΜ M CaMHM ΟΗΟΤΘΜΒ B3HTHH κροη ΛΗΤΟΗβθΛΗΤΟΗOT CHC-τθμη λπη reMOKyTiBTypn, and κροΒΒ ηητθηοηβηο nepeMeinaeTCH οsHHKofí miTaTeJiBHofi. cpefloft, ocymecTBHTCH HHKydHpoBaHHecocy-fla c reMOKyjiBTypoS npz 35-37 ° C kok noaroTOBKa κ ηηΦηηηρο-BaHH »cydKyjn> Type. i -3- ΠρΗ npHCTpofiKe ΗΘ3ΘΚρΗΤ0Γ0 HJIH 38ΚρΗΤ0Γ0 ΒβΗΤΚΤΙΗΐχΗΟΗΗΟΓΟ ΠρΗ “ύποοοάτίθΗΖΗ odecnemiBaeTCH KyjiBTHBHpoBaHHe aapodHHX wih aii-θροόΗΗΧ MHKp00praHH3M0B; B 3aBHCHM0CTH OT HX pOCTa, KOTOpHÉcJie ^ yeT KOHTpoJiHpoBaTB nepe3 οπρβββΛβΗΗΗθ npoMeayTKH βρθμθηη,. ym ym ym ym ^ ^ ^ ^ ^ ^ ^ ^ ^ pe pe pe pe pe pe pe pe pe Ha Ha Ha Ha Ha Ha Ha Ha Ha TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB TB.

Hepe3 pa3JinBHHe npoMejsyTKH ΒρθΜθΗΗ mnpimoM .nepe3 CTepiuiLHyioHTJiy H3 cocysa %jix reMOKyjiBTypH depeTca HHKyÓHpoBaHHaH miTa-TejiLHaa khskoctb, cMeináHHaa c MHKpoopraHB3MaMH, πρηηθμ npodxa . cocysa npoTHKaeTCH. Ojtfia mra ηθοκοζγβκο TBepnax nHTaTeJiBHHx cpeji,HaHeceHHHx Ha ocodse imacTUHKH jwh nanncz Πθτριι h cocTOHUiHe npe-ΗΤ,ψΙΙίθΟΤΒΘΗΗΟ H3 arapa C pa3JIHHHHMH £H$$epeHHHpOBaHHHMH SOÓaBKa- - ΜΗ, ΗΗφΠΙξΗργΤΟΤΟΗ. IlOCJlé HHČDHHHpOBaHIÍH arapOBHX KyJIBTyp OHH emepa3 HHKyóupyioTCH. Οτκρ&amp;ββτβ cocyjíH c reMOKyjiBTypoS jw EHOKy-JLHITHH KpOBLK) MOSHO Ηθρθ3 ΟΠρθβθΛθΗΗΗΘ npOMe&amp;yTKH ΒρθΜΘΗΗ, Ha3-S, 6-11 Η 10 ίξθΗΒ HOCJie B3HTHH KpOBH. JIhuib nocjie pocTa MHKpoopraHH3MOB b cydícyjiBTypax mosho npoBo- :ΛΗτβ ηθτκηθ MBKpoóHazioriraecKiie Hccjre^oBaHHH pa3JiHHHHX opraHH3-MOB.Hepe3 pa3JinBHHe npoMejsyTKH 3ρθΜθΗΗ mnpimoM .nepe3 CTepiuiLHyioHTJiy H3 cocysa% jix reMOKyjiBTypH depot HHKyÓHpoBaHHaH miTa-TejiLHaa khskoctb, cMeinHHaa c MHKpoopraHB3MaMH, πρηηθμ npodxa. cocysa npoTHKaeTCH. Ojtfia mra ηθοκοζγβκο TBepnax nHTaTeJiBHHx cpeji, HaHeceHHHx Ha ocodse imacTUHKH jwh nanncz Πθτριι h cocTOHUiHe npe-ΗΤ; IlOCJH HHDDHHHPOBaHIíH arapOBHX KyJIBTH OHH emepa3 HHKyóupyioTCH. Cτκρ & ββτβ cocyjiH c reMOKyjiBTypoS jw EHOKy-JLHITHH KpOBLK) MOSHO Ηθρθ3 ΟΠρθβθΛθΗΗΗΘ npOMe & yTKH ΒρθΜΘΗΗ, Ha3-S, 6-11 Η 10 ίξθΗΒ HOCJie B3HTHH KpOBH. JIhuib nocjie pocTa MHKpoopraHH3MOB b cydebiBTypax mosho npoBo-: βτβ ηθτκηθ MBKpooHazioriraecKiie Hccjre ^ oBaHHH pa3JiHHHHX opraHH3-MOB.

HejíocTaTOK θτογο ξ3βθοτηογο MeTOsa 3aicjnoHaeTCH b tom, hto ot-ΛΘλιβηηθ 3Tanu b3hthh kpobh η ηηΦεηιιροβηηκθ reMOKyjiBTyp bíhigti-HíHOTCH C nOMODtbD HeCKGWIBKHX HpHÓOpOB, T3K ΗΤΟ ΗΘΤ rápaHTHH Πρθ-SOTBpanjeHKH 33ΓρΗ3ΗΘΗΗΗ πρκ B3HTHH Η ΠρΗ HH^HHHpOBaHHH H 3ace-βθ KyjiLTyp. .HejíocTaTOK θτογο ξ3βθοτηογο METOS b 3aicjnoHaeTCH that HTO OT-ΛΘλιβηηθ 3Tanu b3hthh kpobh η ηηΦεηιιροβηηκθ reMOKyjiBTyp bíhigti-HíHOTCH C nOMODtbD HeCKGWIBKHX HpHÓOpOB, T3K ΗΤΟ ΗΘΤ rápaHTHH Πρθ-SOTBpanjeHKH 33ΓρΗ3ΗΘΗΗΗ πρκ B3HTHH Η ΠρΗ HHHpOBaHHH HH ^ H-3ace βθ KyjiLTyp. .

Ho cHCTewaM jyin reMOKyjiBTyp, fleScTBywix no 3TOMy μθτο^ογ, H3-ΒΘΟΤΗΗ CTOKJIHHHHO COCy^H flJIH reMOKyjiBTyp C ρθ3ΗΗΟΒΟΗ IWH ΜθΤβΛ-jnnecKOH προύκοπ h HanoJiHeHHHe seskoS miTaTejiBHOH cpejxofi. ’·. .JUa Β3ΗΤΙΪΗ KpOBH H3 B6HH H HHOKyjIHIXHH ΚρΟΒΒΚ), a TaKSe B£y-BaHHH ra3ooópa3HHx BeinecTB b cHCTeMy jyiH reMOKyjiBTypH π jvlh. HHČJIfflHpOBaHHH SEEKHX HHTaTeJIBHHX Ορθβ flJIH CyÓKyJIBTyp ΠΟΟΤΟΗΗΗΟTpeóyeTCH προτκκβκΗβ htth οτκρηββηηθ cocysoB jw reMOKyjiBTypa.Ho cHCTewaM jyin reMOKyjiBType, fleScTBywix no 3TOMy μθτο ^ ογ, H3-ΒΘΟΤΗΗ CTOKJIHHHHO COCy ^ H flJIH reMOKyjiBTyp C ρθ3ΗΗΟΒΟΗ IWH ΜθΤβΛ-jnnecKOH προύκοπ h HanoJiHeHHHe seskoS miTaTejiBHOH cpejxofi. '·. .JUa Β3ΗΤΙΪΗ KpOBH H3 B6HH H HHOKyjIHIXHH ΚρΟΒΒΚ), and TaKSe B £ y-BaHHH ra3ooópa3HHx BeinecTB b cHCTeMy jyiH reMOKyjiBTypH π jvlh. HHČJIfflHpOBaHHH SEEKHX HHTaTeJIBHHX Ορθβ flJIH CyÓKyJIBTyp ΠΟΟΤΟΗΗΗΟTpeóyeTCH προτκκβκΗβ htth οτκρηββηηθ cocysoB jw reMOKyjiBTypa.

Bo BpeMH 3thx sTanoB h ocodeHHo Bo Bper.iH onepamtíi c cydKyjiLTy-poií B JiadopaTOpHH B CBH3H C ΠροΤΗΚΒΗΗΘΜ WIH ΟΤΚρΗΒΒΗΗΘΜ npodOK,a TaKse npH MaHHnyjiHpoBafíHH mnpHHaMH, inviaMH ηθ ιιοκώοηθηο sa-ΓρίΙ3ΗΘΕΣΘ. - 4 --Bo BpeMH 3thx sTanoB h ocodeHHo Bo Bper.iH onepamtíi c cydKyjiLTy-poii B JiadopaTOpHH B CBH3H C ΠροΤΗΚΒΗΗΘΜ WIH ΟΤΚρΗΒΒΗΗΘΜ npodOK; - 4 -

JlpyroS HojíocTaTOK sroro Η3Βθοτηογο MeTo&amp;a coctoht b tom, htocjiaó.o pacTyujne kojiohhh όβκτβρπδ e^Ba mojkho odHapyjKHTB KaK bjkhukoh nHTaTejiLHoS cpeae, tbk' h Ha TBepnoM arape. Πο .npyrHM npocneKTaM H3BecTHH cocyflH jveh reMOKyjiLTypa XByx$a3-.HOH ΟΗΟΤΘΜΗ, T.H· CHCTeMH Gibco-Bio-Cult C ΗΘΟΚΟΛΒΚΗΜΗ ΠΗΤΗΤΘΛΒ-hhmh cpe#aMH, odJia£aiomne cneííHajiBHOH προΰκοίί h neperopojiKofi,pa3^eJiflKinea JKHHKHe h TBepnne πκτβτθλβηηθ cpejcn. 3th oocysu jyiareMOKyjiLTypti He #onycKaioT 3aceB npod juih cytíKyjiBTyp b paajnra-ΗΗΘ HHTepBazni ΒρβΜΘΗΗ H TOM CaMHM HOBTOpHOe ΟΤΚρΗΒΒΗΙίθ COCyHOB.Hwaa nHTaTejiLHaji cpe^a, cMemaHHaH c kpoblio, οτοκβθτ πρπ Bpa-meHHH cocyna b npejuiasHa^eHHyio &amp;τιη 3Toro KaMepy, ομηββθτ h hh-$nnnpyeT TBepwyio arapoByio $a3y, HaHeceHHyro Ha neperopoíCKy, hnocjie oToro ohhtb cJiHBaoTCH b npesHa3HaHeHHyio jyw θτογο KaMepycocyna.JlpyroS HojíocTaTOK sroro Η3Βθοτηογο MeTo & coctoht b tom, htocjiaó.o pacTyujne kojiohhh όβκτβρπδ e ^ Ba mykho fromHapyjKHTB KaK bjkhukoh nHTaTejiLHoS cpeae, tbk 'h Ha TBepnoM arape. Πο .npyrHM npocneKTaM H3BecTHH cocyflH jveh reMOKyjiLTypa XByx $ A3 .HOH ΟΗΟΤΘΜΗ, T.H. · CHCTeMH Gibco Bio-Cult ΗΘΟΚΟΛΒΚΗΜΗ ΠΗΤΗΤΘΛΒ C-CPE hhmh # AMH's released £ aiomne cneííHajiBHOH προΰκοίί neperopojiKofi H, PO3 H ^ eJiflKinea JKHHKHe TBepnne πκτβτθλβηηθ cpejcn. 3th oocysu jyiareMOKyjiLTypti He #onycKaioT 3aceB npod juih cytíKyjiBTyp b paajnra-ΗΗΘ HHTepBazni ΒρβΜΘΗΗ H TOM Camhi HOBTOpHOe ΟΤΚρΗΒΒΗΙίθ COCyHOB.Hwaa nHTaTejiLHaji CPE ^ a, c cMemaHHaH kpoblio, οτοκβθτ πρπ Bpa-meHHH cocyna b npejuiasHa eHHyio ^ &amp; τιη 3Toro KaMepy, ομηββθτ h hh- $ nnnpyeT TBepwyio arapoByio $ a3y, HaHeceHHyro Ha neperopoCy, hErO oOtOr ohhtb cJiHBaoTCH b npesHa3HaHeHHyio jyw θτογο KaMepycocyna.

HeaocTaTKOM θτοη ,HByx$a3HoS chctomh jvih reMOKyjiLTypu ηβττηθτοηto, hto b CBH3H c KOHCTpyKiínea cocyEa, HMeromero οτηοοιιτθλβηοÓOT&amp;myiO θΜΚΟΟΤΒ, ΗθΤ HpHMOS CBH3H MeJKJQT BeHOÉ, CHCTeMO0 B3HTHHKPOBH H CHCTGMOS flJLH reMOKyJIBTypH. Β3ΗΤΗβ KpOBH ,H3 ΒΘΗΗ, a 3Ha-HHT H-HQUana KPOBH B 3TOT COCyA HHH ŽJiaKOH JUH reMOKyjTBTypHocymecTBJífleTCfl, γλθβηημ odpa3OM, mnpHuoM tojilko cocysoB cHedOJTBIIIOH eMKOCTBÍO HOCpeaCTBOM nmaHTOBOrO COeaHHeHHH c uruioS Β3ΗΤΗΗ KpOBH Η7ΓΗ ΚΟΜ&amp;ΖΓβΚΤβ RXX Β3ΗΤΗΗ KpOBH.HeaocTaTKOM θτοη, HByx $ a3HoS chctomh jvih reMOKyjiLTypu ηβττηθτοηto, HTO CBH3H b c KOHCTpyKiínea cocyEa, HMeromero οτηοοιιτθλβηοÓOT &amp; myiO θΜΚΟΟΤΒ, ΗθΤ HpHMOS CBH3H MeJKJQT during, CHCTeMO0 B3HTHHKPOBH H CHCTGMOS flJLH reMOKyJIBTypH. Β3ΗΤΗβ KpOBH H3 ΒΘΗΗ and 3 ha HHT-H HQUana KPOBH B 3This Coca HHH ŽJiaKOH JUH reMOKyjTBTypHocymecTBJífleTCfl, γλθβηημ odpa3OM, mnpHuoM tojilko cocysoB cHedOJTBIIIOH eMKOCTBÍO HOCpeaCTBOM nmaHTOBOrO COeaHHeHHH c uruioS Β3ΗΤΗΗ KpOBH Η7ΓΗ ΚΟΜ &amp; ΖΓβΚΤβ Rxx Β3ΗΤΗΗ KpOBH.

HecMOTpn Ha to, hto sth j£Byxč&amp;a3HHe čmaKOHH λλη reMOKyjiBTypodecneHEBaioT doJiee cKopaS ροοτ kojiohhh na arape, a kpobb depeT-ch οτηθνΤ&amp;Ηο, BO3HHKaeT onacHocTB 3arpH3fíeHHH. Jlajiee, npn τεκοδcHCTeMe jvlh reMoKyjiBTypH saTpynHHeTca οπτηηθοκοθ oTpaseHne od-pa30BaHHH kojiohhh bo BpeMH HHKydaiw, a b cbh3H c sthm ocodeH-ho 3aTpynHHeTca MURpodHO^rorinecKoe HccJieflOBamie cjiadopacTymiixKyjiBTyp. IIoaTOMy HeodxoflHMO πρπ onpe^ejreHHHX BHnax reMOKyjiBTypTBepnyio arapoBys &amp;a3y ποβτορηο oMHBaTB nepe3 onpeaejreHHne npo-MesyTKH BpéMeHH 3KHHKOS. CMeCBK HHTaTeJIBHOS Cpe,HH H KPOBH. Πο DĚ-O3 2221096 h DE-os 2332133 b KanecTBe odopysoBaHHH jpwB3HTHH KpOBH Ι13ΒθΟΤΗΗ HHCTpyMeHT B3HTHH KpOBH H HTJia JtflH B3HTHHaH3JIH30B KpOBH.HecMOTpn Ha to, hto sth j £ Byxc & a3HHe chmaKOHH λλη reMOKyjiBTypodecneHEBaioT doJiee cKopaS ροοτ kojiohhh on arape, and kpobb depeT-ch οτηθνΤ & Ηο, BO3HHKaeT onacHocTB 3arpH3fiHHH. JpnHHeTca οπτηηθοκοθ oTpaseHne od-pa30BaHHH kojiohhh bo BpeMH HHKydaiw, a b cbh3H c sthm ocodeH-ho 3aTpynHHeTca MURpod ^ rorinecKoe HccJieflOBamie cjiadopacTymiixKyjiBTyp. IIoaTOMy HeodxoflHMO πρπ onpe ^ ejreHHHX BHnax reMOKyjiBTypTBepnyio arapoBys &amp; a3y ποβτορηο oMHBaTB nepe3 onpeaejreHHne npo-MesyTKH BpéMeHH 3KHHKOS. CMeCBK HHTaTeJIBHOS Cpe, HH H KPOBH. Πο Ě3 O10 2221010 h10 DE1010101010 b10101010101010101010 22210101010101010101010101010101010101010101010101010101010101010101010101010101010101010101010101010----------------------------------------

CorjiacHo Ha3BaHH0My MeTojny b3hthh kpobh HHCTpyMeHT jj;jih B3hthhCOCTOHT H3 ΗΓΛΗ C flepftaTOJieM, npiraéM ΚΟΗΘΗ HTJIH, HaXOAHiqHSCH BHyTpH, OKpyHeH KJianaHOM C ΒβΖΤΖΤΙΖΖΖΟΖΗΗΜ ΠρΖΟΠΟΟΟ&amp;ΙβΖΖβΜ.:Knanaz htjth mokzo nepeBonzTL Z3 onzoro zojiosezzz, b κοτοροΜ .oóecnezzBaeTCz ΒθζτζτιζίίΚΗ (zepe3 yzKzfi zpoxon) z nóKa3 'κροεζ,b apyroe, b κοτοροΜ npenoTBpainaeTGH npoxosnezze B03nyxá mizκροΒΖ. .HepsaTeJiB. coctoht H3 ockoezoh zacTz b Bzne TpydozKK z zacTz cBHyTpeHHefí KOHiraecKOĚ noBepxHocTBD. Tpydozza cjiyszT jmz zpen-nezzz cocyna, no KOTopoiiiy 3BaKyzpyeTCH reMOKynBTypa, c προτΗ-Kaer.ioS ζροόκοζ. KozzzecKaz zoBepxzocTL cjiyszT b zazecTBe yno-pa jvlh cocyna c KyjiETypoz, Korná zozez htjih, npoTHKaiomzii npoó-Ky, προτκζβτ ee. CHCTeMH B3ZTZH ΚρΟΒΗ Ζ3ΒΘ0ΤΖΗΜ 0Ópa30M ΟΟβχΐίΗΗθΤΟΗ C CZCTeMOZjyiz rewoKyjiBTypH c homoiulio θτογο κρθιυίθΗζζ. - ΠοηοόΗΗΜ se oópaaoM ycTpoeHa zrjia, 3amwHzaH b οοοτβθτοτβζζc de-os 2332133; zepenHzz zacTL zrna ΒΒοηχτοζ b BeHy, a προτζ-BononosHaa zacTL - b codzpaTenLHHfí cocyn jw reMOKyjrLTypa. 3Tazrna OTJizzaeTcz oazoxozobhm mianaHOM, npenoTBpamajamHM BO3BpaTshhkocth Z3-coózpaTeJiBHoro cocyna b czcTewy KpoBooópameHZH za-HzezTa, a Tarase cpencTBOM ηπζ Harjrznzoro noza3a nBzseHZH κροΒΖ.y zrna zr.ieeTcz Tarase nepsaTem,, zpzcnocoájreaHHfi κ čoopMe coóz-paTeJiLHoro cocyna jpra reMOKyjiLTypa,- npencTaBJBnonrzH codoS npez-MymecTBeHHO nojmfi zznzzup jvlh KperaieHzz 3τογο cocyna. IlocjrenpoKona Beza οζοτθμη b3htzz kpobh coenzHzeTcz c οζοτθμοη nnHreMOKyjE&amp;Typa nocpencTBOM zpeznezza. HenocTaTKOM θτογο coenzHe-zzh, Tarase Kaz z za3BaHZoro βηιιιθ, .zBJízeTez to, zto yBeJizzzBa-θτοη 3arpfl3HfleM0CTB Bcez,οζοτθμη, a Tarase cjioszoctl nanL3OBa-HHH yCTpOHCTBaMZ. Πο de-ps III0357 zaBecTHH Bazypme aMnyjia jw CTepzzLzoroB3HTZH κροΒΖ z npyrzx szHKocTez; menza, 3azpaTaz BBepxy, za-caseza na zpoózy c zrnoK, Bnamiezzyio c zzsHero zozua aMzyna,z zrjiy; zz3 btzx ai-myji sazjiasjízeTcz non bhcokzm BazyyMOM. 3tz BaKýyuHHe aMnyjia cnysaT zczmozzTeJiLHo jyra bsztzh kpobz;OTcocazzaz κροΒΒ nojesHa nocne θτογο óhtl BBeneHa b oTnenEzyK)czcTewy jyw reMozyjiLTypH. ' f - 6 -CorjiacHo Ha3BaHH0My MeTojny b3hthh kpobh HHCTpyMeHT jj; south B3hthhCOCTOHT H3 ΗΓΛΗ C flepftaTOJieM, npiraéM ΚΟΗΘΗ HTJIH, HaXOAHiqHSCH BHyTpH, OKpyHeH KJianaHOM C ΒβΖΤΖΤΙΖΖΖΟΖΗΗΜ ΠρΖΟΠΟΟΟ &amp; ΙβΖΖβΜ.: Knanaz htjth mokzo nepeBonzTL Z3 onzoro zojiosezzz b κοτοροΜ .oóecnezzBaeTCz ΒθζτζτιζίίΚΗ (zepe3 yzKzfi zpoxon) of nóKa3 'κροεζ, b apyroe, b κοτοροΜ npenoTBpainaeTGH npoxosnezze B03nyxá mizκροΒΖ. .HepsaTeJiB. coctoht H3 oh oh z ac b b b py py py py py py py py B B B B B B B B B B B B noBepxHocTBD. Tpydozza cjiyszT jmz zpen-nezzz cocyna, no KOTopoiiiy 3BaKyzpyeTCH reMOKynBTypa, c προτΗ-Kaer.ioS ζροόκοζ. KozzzecKaz zoBepxzocTL cjiyszT b zazecTBe yno-pa jvlh cocyna c KyjiETypoz, Korna zozez htjih, npoTHKaiomzii npoo-Ky, προτκζβτ ee. CHCTeMH B3ZTZH ΚρΟΒΗ Ζ3ΒΘ0ΤΖΗΜ 0Ópa30M ΟΟβχΐίΗΗθΤΟΗ C CZCTeMOZjyiz rewoKyjiBTypH c homoiulio θτογο κρθιυίθΗζζ. - ΠοηοόΗΗΜ se oópaaoM ycTpoeHa zrjia, 3amwHzaH b οοοτβθτοτβζζc de-os 2332133; zepenHzz zacTL grains ΒΒοηχτοζ b BeHy, and προτζ-BononosHaa zacTL - b codzpaTenLHHfí cocyn jw reMOKyjrLTypa. 3Tazrna OTJizzaeTcz oazoxozobhm mianaHOM, npenoTBpamajamHM BO3BpaTshhkocth Z3 coózpaTeJiBHoro cocyna b czcTewy KpoBooópameHZH per-HzezTa and Taras cpencTBOM ηπζ Harjrznzoro noza3a nBzseHZH κροΒΖ.y grains zr.ieeTcz Taras nepsaTem ,, zpzcnocoájreaHHfi Coop κ-COOZ paTeJiLHoro cocyna jpra reMOKyjiLTypa - npencTaBJBnonrzH codoS npez -MymecTBeHHO nojmfi zznzzup jvlh KperaieHzz 3τογο cocyna. IlocjrenpoKona Beza οζοτθμη b3htzz kpobh coenzHzeTcz c οζοτθμοη nnHreMOKyjE & Typa nocpencTBOM zpeznezza. HenocTaTKOM θτογο coenzHe-zzh, Tarase Kaz z za3BaHZoro βηιιιθ, .zBJízeTe not that yBeJizzzBa-θτοη 3arpfl3HfleM0CTB Bcez, οζοτθμη, and Tarase cjioszoctl nanL3OBa-HHH yCTpOHCTBaMZ. Πο de-ps III0359 zaBecTHH Bazypme aMnyjia jw CTepzzLzoroB3HTZH κροΒΖ z npyrzx szHKocTez; menza, 3azpaTaz BBepxy, za-caseza for c grain, Bnamiezzyio c zzsHero zozua aMzyna, z zrjy; zz3 btzx ai-myji sazjiasjizeTcz non bhcokzm BazyyMOM. 3tz BaKýyuHHe aMnyjia cnysaT zczmozzTeJiLHo jyra bsztzh kpobz; OTcocazzaz κροΒΒ nojesHa nocne θτογο óhtl BBeneHa b oTnenEzyK) czcTewy jyw reMozyjiLTypH. 'f - 6 -

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CooTBeTCTByiomee H30dpeTeHHKi odopynoBaHHe Xjlh βηηβαθηηη daKTe-pHMHg, COCTOfDHee H3 CHCTeMH B3HTHH KpOBH Η CHCTeMH RKR ΓβΜΟ-KyjiBTypa, xapaKTepn3yeTCH τθμ, ήτο cocyu οηοτθμη ajih reMOKyjiB-Typa npeflCTaBJraeT codofi ai-myjiy c KOJiBneodpa3HHM · cyseHiíeM h htonoJio&amp;eHne θτογο KOJimeodpa3Hóro cyjxeHHH.no jvruHe ai.myjm npn- cnocoÓJieHO κ TpeóyioiueMycjť οοοτηοπιθηηκ) κοληηθοτβη τβθραο2 η -OTAKOÍ1 ΠΗΤΗΤΌΛΒΗΟΗ ΌρθΑΗ» ΠρθΙΠ.ψΙΙΙΘΟΤΒΘΗΗΟ 1:3. Jíajiee, oÓopýaoBáHne, cooTBeTCTByiomee Η3θόρβτβΗΗκ, xapaKTepiray-θτοη τβΜ, ήτο b cocyAe οηοτθμη ajih reMOKyjiLTypH scHAKaH h TBep—Aan imTaTejiLHaH cpe.ua pacnaHOJKeHH noonepeAHo η πρπ ποαοκθηηη' cocy.ua b AHana30He HaimoHa O -135° conpHKacajOTcn Apyr c ApyTOM,h ήτο odteM η. πατιο&amp;θΗΗθ TBepAoS BUTaTejiLHOH cpejxH (ShkcnpyeTcaKOJimeo(5pa3HHM cyzeHHeM. y K0Jrmeoópa3Horo cyxeHHír Ha noBepx-hocth HMeeTCH nepMaHeHTHoe kojibiío, no3BOJinoisee npoH3BéCTHrjia£Koe οταθαθηηθ odenx ήηοτθη cocyna ahh reMOKyABTypa de3 oc-kojikob. JínaweTp nponycKHoro OTBepcTHH KOJimeodpa3Horo cysceHásHaxoflHTCfl b npe;n;ejrax 6^ 10 mm, t.k. κοληήθοτβο h noJioxteHnearapoBoií $a3H 3aAaHO Hanajiow pannyca cysceHHH οθήθηηη. OdopyAO-BaHse, cooTBeTCTBywe H3odpeTeHino, xapaKTepireyeTCH eine η τβΜ,ήτο cocyA CHCTeMH jyin reMOKyABTypH coeAHHeH c cncTeMofí b3hthhKpOBH OAáCTKHHOH TpydOHKOH ΠρθϋΜ^ΘΟΤΒθΗΗΟ H3 ΜΗΓΚΟΗ IUiaCTMaC-ch h npeácTaBJíaeT codofi 3βκρπτγιο ποοαθαοβ3τθαβηο pacnoJioseHHyKiHaCTL, H OCB CHCTeMH B3HTHH KpOBH paCHOJIOKeHa ΠΟΑ yrJIOM K OCHοΗοτβΜΗ ααη reMOKyjiBTypH h ee moshó ποβορ&amp;ήηβητβ b jnodoM HanpaB·ΑΘΗΗΗ.CooTBeTCTByiomee H30dpeTeHHKi odopynoBaHHe Xjlh βηηβαθηηη Dakto-pHMHg, COCTOfDHee H3 CHCTeMH B3HTHH KpOBH Η CHCTeMH RKR ΓβΜΟ-KyjiBTypa, xapaKTepn3yeTCH τθμ, ήτο Coca οηοτθμη ajih reMOKyjiB-Typa npeflCTaBJraeT codofi ai-wash c KOJiBneodpa3HHM · cyseHiíeM h htonoJio &amp; eHne θτογο KOJimeodpa3Hóro cyjxeHHH.no jvruHe i jm jm jm jm κ jm κ κ κ pe pe:::::::::::::::::: 1: 3. Jíajiee, oÓopýaoBáHne, cooTBeTCTByiomee Η3θόρβτβΗΗκ, xapaKTepiray-θτοη τβΜ, ήτο b cocyAe οηοτθμη ajih reMOKyjiLTypH scHAKaH h TBep-Aan imTaTejiLHaH cpe.ua pacnaHOJKeHH noonepeAHo η πρπ ποαοκθηηη 'cocy.ua b AHana30He HaimoHa about -135 ° C conpHKacajOTcn pyr ApyTOM h ήτο odteM η. πατιο &amp; θΗΗθ TBepAoS BUTaTejiLHOH cpejxH (ShkcnpyeTcaKOJimeo (5pa3HHM cyzeHHeM. y K0Jrmeoópa3Horo cyxeHHír Ha noBepx-hocth HMeeTCH nepMaHeHTHoe kojibiío, no3BOJinoisee npoH3BéCTHrjia £ Koe οταθαθηηθ odenx ήηοτθη cocyna AHH reMOKyABTypa DE3 alpha-kojikob. JínaweTp nponycKHoro OTBepcTHH KOJimeodpa3Horo cysceHásHaxoflHTCfl b NPE n; ejrax 6 ^ 10 mm tk κοληήθοτβο h noJioxteHnearapoBoií $ and3H 3aAaHO Hanajiow pannyca cysceHHH οθήθηηη. OdopyAO-BaHse, cooTBeTCTBywe H3odpeTeHino, xapaKTepireyeTCH eine η τβΜ, ήτο Coca CHCTeMH jyin reMOKyABTypH coeAHHeH c cncTeMofí b3hthhKpOBH OAáCTKHHOH TpydOHKOH ΠρθϋΜ ^ ΘΟΤΒθΗΗΟ H3 ΜΗΓΚΟΗ IUiaCTMaC-chloro h npeácTaBJíaeT codofi Β y CB CB CB CB CB β β CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB CB

CooTBeTCTByHqas H3odpe Temno cncTeMa b3hthh κροΒΗ xapaKTepirayeT-CH ΤβΜ, ΉΤΟ HHSHHH HaCTB TpydOHKH HHBeKUHOHHOfi ΗΓΑΗ yjyiHHeHa.TaK, ήτο TpydoHKa oxBaTHBaeT odJiaMHBaeMHH οτθκτιηηηηη κοηθπ co-cysa οηοτθμη ajih reMOKyABTypH, djiaroAapn newy odJiai.nmaTB οτθκ-JLHHHHH KOHeiX MOSHO ΘΠίθ £0 B3HTHH KpOBH. ΠΟΜΗΜΟ ΤΟΓΟ, odjiaMHBaeMHH ΚΟΗθΠ CHCTeMH Β3ΗΤΗΗ KpOBH, SAaCTHH-Han TpydoHKa π οηοτθμη b3hthh κροΒΗ OKpyseHH npiweraioinHM kojt-ΠβΉΚΟΜ AJIH 33ΰξΚΤΗ ΟΗΟΤΘΜΗ Β3ΉΤΗΗ KpOBH. ITpHMep ocymecTBJíeHHg H3odpeTeHHg JIoctohhctbo MeTOAa h oóopyAOBaHHH, cooTBeTCTByioiiíera H3odpeTe-HH30, 3aKTK>HaeTCH B TOM, ΉΤ0 djrarOAapn Κ0Μ0ΗΗΗρ0ΒΗΗΗΕ> MeŤO£a HOdopyAOBaHHH CHCTeMH B3HTHH KPOBH H CHCTeMH A&amp;H reívlOKyJILTypHco3AaeTcn odBeAHHeHHé κθοκολγβκηχ ao ότογο pa3po3fíeHHHX· 9TanoBπρπ ΟΑΗΟΒρβΜβΗΗΟΜ ΚΗΉβΟΤΒβΗΗΟΜ yJiyHUieHHH MeTOAa B3HTHH H aHa- , JIK3a KpOBH C nOMOIUBIO CBOÓOflHOro OT 3βΓρΗ3ΗβΗΗΗ H yftOÓHOrO BnOJIL3OBaHHH OÓOpyftOBaHHH. ΠθΜΗΜΟ ΤΟΓΟ, dJiaTO^apH ΠρΗΜΘΗβΗΗΚ)BUcoKOKaHečTBeHffflX nuTaTeJíLHiix cpefl c aHTHdaKTepHniWHLiMH #o-óaBKawH h TBepnnx γοπήτθλβηηχ cpe# c HH&amp;HKaTopaMH—κρβοητθ&amp;ημηCQ3flaeTCfl B03M0KH0CTB B3HTHH ΜθΗΒΙΗθΓΟ ΚΟΛΗΗΘΟΤΒΗ KpOBH H HHČĎH—iXHpoBaHiiá ee KyjiBTypawH daKTepHŠ. B οοοτβθτοτβηη c coctsbom ifflTaTeJiBHoS cpe^H, coftepEamefi θτηonpejieJieHHHe aodaBKH, mojkho BupamnBaTB tojibko HedoJiBiuoe kojih-ΗΘΟΤΒΟ MHKpOOpraHH3MOB,. Τ.Θ. dpaTL ΜΘΗΒΠΙθβ KOJIIFíeGTBO KpOBH H-odieM odenx naoTeS cocyna chotomh &amp;eh rewoKyjiBTypH coxpaHHTLΜβΗΒΠΙΗΜ, ΗβΜ y Η3ΒΘ0ΤΗΗΧ yjKe COCyHOB ftJIH reMOKyJIBTyp.CooTBeTCTByHqas H3odpe Dark cncTeMa b3hthh κροΒΗ xapaKTepirayeT CH ΤβΜ, ΉΤΟ HHSHHH HaCTB TpydOHKH HHBeKUHOHHOfi ΗΓΑΗ yjyiHHeHa.TaK, ήτο TpydoHKa oxBaTHBaeT odJiaMHBaeMHH οτθκτιηηηηη κοηθπ co-Cys οηοτθμη ajih reMOKyABTypH, djiaroAapn newy odJiai.nmaTB οτθκ-JLHHHHH KOHeiX Moshe ΘΠίθ £ 0 B3HTHH KpOBH. ΠΟΜΗΜΟ ΤΟΓΟ, odjiaMHBaeMHH ΠθΠ CHCTeMH Β3ΗΤΗΗ KpOBH, SAaCTHH-Han TpydoHKa π οηοτθμη b3hthh κροΒΗ OKpyseHH npiweraioinHM kojt-ΠβΉΚΟΜ AJIH 33ΰξΚΤΗ ΟΗΟΤΘΜΗ Β3ΉΤΗΗ KpOBH. ITpHMep ocymecTBJíeHHg H3odpeTeHHg JIoctohhctbo by site h oóopyAOBaHHH, cooTBeTCTByioiiíera H3odpeTe-HH30, 3aKTK> HaeTCH B TOM ΉΤ0 djrarOAapn Κ0Μ0ΗΗΗρ0ΒΗΗΗΕ> Meto £ and HOdopyAOBaHHH CHCTeMH B3HTHH KPOBH H CHCTeMH A &amp; H reívlOKyJILTypHco3AaeTcn odBeAHHeHHé κθοκολγβκηχ and ότογο pa3po3fíeHHHX · 9TanoBπρπ ΟΑΗΟΒρβΜβΗΗΟΜ ΚΗΉβΟΤΒβΗΗΟΜ yJiyHUieHHH by site B3HTHH H Aha -, JIK3a KpOBH C nOMOIUBIO CBOÓOflHOro OT 3βΓρΗ3ΗβΗΗΗH yftOÓHOrO BnOJIL3OBaHHHOOpyftOBaHHH. ΠθΜΗΜΟ ΤΟΓΟ, dJiaTO ^ and pH ΠρΗΜΘΗβΗΗΚ) BUcoKOKaHečTBeHffflX nuTaTeJíLHiix cpefl aHTHdaKTepHniWHLiMH c # o-óaBKawH TBepnnx γοπήτθλβηηχ CPE h # c HH &amp; HKaTopaMH-κρβοητθ &amp; ημηCQ3flaeTCfl B03M0KH0CTB B3HTHH ΜθΗΒΙΗθΓΟ ΚΟΛΗΗΘΟΤΒΗ KpOBH HHČĎH H-iXHpoBaHiiá KyjiBTypawH daKTepHŠ ee. B οοοτβθτοτβηη c coctsbom ifflTaTeJiBHoS cpe ^ H, coftepEamefi θτηonpejieJieHHHe aodaBKH, my BupamnBaTB tojibko HedoJiBiuoe kojih-ΗΘΟΤΒΟ MHKpOOpraHH3MOB ,. Τ.Θ. dpaTL -ΠΙθβ KOJIIFíeGTBO KpOBH H-odieM odenx naoTeS cocyna chotomh &amp; eh rewoKyjiBTypH coxpaHHTLΜβΗΒΠΙΗΜ, ΗβΜ y Η3ΒΘ0ΤΗΗΧ yjKe COCyHOB ftJIH reMOKyJIBType.

Diaro^apH ojmoBpeMeHHofi ηηοκυληιϊηη jkhukoh η TBepnoS πιίτητθηβ-hoh cpeš, a Tarase ÓJiarosapa onTiraecKOMy npeftCTaBnem) pa3MH0-ΣίβΗΐΐΗ daKTepnĚ odecneHBBaeTCH 3Ηηήητθ^ιβηοθ ynpomeHHe BceroweTQfla, a TaKse coKpameHHe Η3Αβρκβκ Ha patíoTH no daKTepiiojiorHH.HenocpeflCTBeHHaa nojjana κροΒΗ b XByx$a3HHS cocyn jyin reMOKyjiL-TypH, dHCTpaH HHOKyjiHHHH odenx rorraTejiLirax cpe# nocjie b3hthhκροΒΗ h HHKydaiTO KyjiBTyp' daKTepuS b AByx ήηοτηχ cocysa jyiHreMOKyjiBTypH de3 nocneftyioinero nepeHeceHEH h noTowy de3 3arpH3-ΗβΗΗΗ Π03Β0ωΐΗ3Τ ΠρΟΒΘΟΤΗ dHCTpHH Η Η8£β3ΚΗΚ&amp; #ΗΗΓΗΟ3.Diaro ^ and pH ojmoBpeMeHHofi ηηοκυληιϊηη jkhukoh η TBepnoS πιίτητθηβ-Hoh CPEs, and Taras ÓJiarosapa onTiraecKOMy npeftCTaBnem) pa3MH0-ΣίβΗΐΐΗ daKTepnĚ odecneHBBaeTCH 3Ηηήητθ ^ ιβηοθ ynpomeHHe BceroweTQfla and taksa coKpameHHe Η3Αβρκβκ Ha patíoTH well daKTepiiojiorHH.HenocpeflCTBeHHaa nojjana κροΒΗ b XByx $ a3HHS cocyn jyin reMOKyjiL- typhi dHCTpaH HHOKyjiHHHH odenx rorraTejiLirax CPE # nocjie b3hthhκροΒΗ h HHKydaiTO KyjiBTyp 'daKTepuS b AByx ήηοτηχ cocysa jyiHreMOKyjiBTypH DE3 nocneftyioinero nepeHeceHEH h noTowy DE3 3arpH3-ΗβΗΗΗ Π03Β0ωΐΗ3Τ ΠρΟΒΘΟΤΗ dHCTpHH Η Η8 £ β3ΚΗΚ &amp;# ΗΗΓΗΟ3.

OdBejumeHHe jkhhkoé η τβθρηοη ΠΗτατβΛΒΗοδ cpea b cooTBeTCTByio—meM H3otípeTeHEio cocyse jwlh reMOKyjiLTypa no3BOHHeT jyin ycKope-hhh pocTa daKTepnS y onpe^ejreHHHx kojtohuS opomaTL tíazree iizmMeHee noBepxHocTB TBep®o0 arapoBOH $a3H nepe3 onpeAeHefíHHe npo-KiesyTKH βρομθηη tohkhm cjioeM khukoh imraTeJiiHofi ope^n npn Han-jiohhom noHoaceHHH cocyHa.OdBejumeHHe jkhhkoé η τβθρηοη ΠΗτατβΛΒΗοδ CPEA b cooTBeTCTByio-MEM H3otípeTeHEio cocyse jwlh reMOKyjiLTypa no3BOHHeT jyin ycKope-hhh number daKTepnS onpe y ^ ejreHHHx kojtohuS opomaTL tíazree iizmMeHee noBepxHocTB TBep®o0 arapoBOH $ and3H nepe3 onpeAeHefíHHe NPO-KiesyTKH βρομθηη tohkhm cjioeM khukoh imraTeJiiHofi ope ^ n npn Hanohohom noHoaceHHH cocyHa.

DiarosapH MeTojiy, npejyiaraeMOMy κβοόρβτβΗΗβΜ, μοκθτ He ποτρβ-doBaTBca OTflejii.Hoe cydByjiBTHBHpoBaHHe H3 cocyna c reMOKyHLTy-pofi η τθμ caťMM He noTpedyioTCH TexHHKO-TexHOTiornHecKHe 3aTpa-th. KjiarojiapH KOJH»ueodpa3HpMy cysemro mojkho nocne pa3MHO»eHHHdaKTepnS Jierno pa3flejiHTL jjeTaziH cocy^a c rewoKyziLTypoH λζιη .Toro, HTodu HccjiesoBaTL ode ΠΗΤΒτβΠΒΗΗβ cpejni owjibho Apyrστ jípyra. Enaro^apn Kozimy Kazrmeodpa3Horo cyseHHH mojkho προ- ·HŠBecTH iuiajíKHíí pa3jroM no hhhhh, He noBpejíHBmiicL npu stom. - 9 - i ‘ · * . ' ' ·Ar ο ο oe ar ar ar ar ar ar ar ar ar ar ar 3 3 3 3 3 3 H H H H H H H H H H H H H a a a c a KjiarojiapH KOJH »ueodpa3HpMy cysemro mojkho nocne pa3MHO» eHHHdaKTepnS Jierno pa3flejiHTL jjeTaziH cocy ^ a c rewoKyziLTypoH λζιη .Toro, HTodu HccjiesoBaTL od ΠΗΤΒτβΠΒΗΗβ cpejni owjibho Enaro ^ apn Kozimy Kazrmeodpa3Horo cyseHHH my hrhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhhh - 9 - i · *. '' ·

Ha HHaecTOHmeM npHMěpe- πρηβΟλητοη Ή ο0έηοηηθτοη μθτοβ η odopy-AOBamie, npejvraraeivMe η300ρθτθηηθμ. ΠρΗΜβρ ηηγλή^ηο ποηοηηθτοηHa npnnaraeMOM nepTese; Ha ηθμ npe^cTasjíeHo: ΦΗΓ. CxeMHTHHeCKOe ΠρβΑΟΤΘΒΛβΗΗθ ΠρΟβΟΛΒΗΟΓΟ οθηθηηηΜΟΑ&amp;ΚΗ, npe^iaraeMOH'Η3θύρθΤΘΗΗΘΜ.Ha HHaecTOHmeM npHMepe- πρηβΟλητοη Ή ο0έηοηητοβ η odopy-AOBamie, npejvraraeivMe η300ρθτθηηθμ. ΠρΗΜβρ ηηγλή ^ ηο ποηοηηθτοηHa npnnaraeMOM nepTese; Ha ηθμ npe ^ cTasjíeHo: ΦΗΓ. CxeMHTHHeCKOe ΠρβΑΟΤΘΒΛβΗΗθ ΠρΟβΟΛΒΗΟΓΟ οθηθηηηΜΟΑ &amp; pe, npe ^ iaraeMOH'Η3θύρθΤΘΗΗΘΜ.

CorJiaCHO ΜΘΤΟΑΥ HCCJieflOBaHHH daKTepHMHS .B ΚρΟΒΗ Η3 BQHH naiXH-θΗΤΗ B £03Hp0BaHH0M ΚΟΤΙΗΗΘΟΤΒΘ Ήθρβ3 CHCTeMy B3HTHH KpOBH, CHC-Téwy HTJI Η7ΠΪ mnpimOB ΟΠρθΑΘΛΘΗΗΟΗ KOHCTpyKmiH OTCaCHBaeTCHκροΒΒ λπη aHajiH3a b ctokjihhhhS cocynATiH reMOKyJTLTypH noa Bany-yMOM JWH 3anOJIHeHHHH ra30M H 3βΤβΜ CMeiIIHBaeTCH C W^KOÍÍ ΠΗΤβ-TeJiLHoS cpejioíi, coflepKameĚCH b cocyne. OTcocaHHan τβκΗΜ odpa3OM ' · κροΒΒ HHOKyjmpyeT jnwKyio cpeřcy c aHTHdaKTepwnXHUMii λο0ηβκημη, / ΠρΗΗΘΜ ΟΛΗΟΒρθΜΘΗΗΟ CMeCBJO KpOBH H ΠΗΤΗΤβΛΒΗΟΗ CpeflH HHOKyJIHpy- eTCH TBepnan ΠΗτβτβΛΒΗθΗ cpena b cocyne c reMOKyn&amp;TypoS. IlocjreθτοΓο npn onpeaejieHHofí TeMnepaType h ΗβκηοΗΗΟΜ nonoaeHHH cocy-sa, b eaBECHMOCTH ot ncanenyeMHx KyjiLTyp daKTepuS b odenx HHTa-τθλβηηχ cpenax BupadaTHBaeTcn daKTepnajiBHan Kym&amp;Typa. BnaronapníodaBJíeHHfo HHjwaTopa-KpacHřejin npeHMymecTBeHHO κ TBepnoS nzTa—TejiBHOĚ cpene npenocTa&amp;nneTcn bo3mohhoctb odHapyzeHnn daKTepnS,pa3MHOKHBIUHXCH B ΟόθΗΧ ΟρββθΧ.CorJiaCHO ΜΘΤΟΑΥ HCCJieflOBaHHH daKTepHMHS .B ΚρΟΒΗ Η3 BQHH naiXH-θΗΤΗ B £ 03Hp0BaHH0M ΚΟΤΙΗΗΘΟΤΒΘ Ήθρβ3 CHCTeMy B3HTHH KpOBH, CHC-Téwy HTJI Η7ΠΪ mnpimOB ΟΠρθΑΘΛΘΗΗΟΗ KOHCTpyKmiH OTCaCHBaeTCHκροΒΒ λπη aHajiH3a b ctokjihhhhS cocynATiH reMOKyJTLTypH Noa Bany yMOM JWH-3anOJIHeHHHH ra30M H 3βΤβΜ CMeiIIHBaeTCH CW ^ KOH ΠΗΤβ -TeJiLHoS cpejioíi, coflepKameĚch b cocyne. T oca ΒΒ HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO HO ep ep ep ep ep ep ep ep ep ep ep ep ep IlocjreθτοΓο npn onpeaejieHHofí TeMnepaType h ΗβκηοΗΗΟΜ nonoaeHHH cocy-sa, b eaBECHMOCTH ot ncanenyeMHx KyjiLTyp daKTepuS b odenx HHTa-τθλβηηχ cpenax BupadaTHBaeTcn daKTepnajiBHan Kym &amp; BnnnnwnHHfo HHjwaTopa-KpacHeave npeHMymecTBeHHO κ TBepnoS ntTe — THeiBHoT cpene npenocTa & nneTcn bo3mohhoctb odHapyzeHnn daKTepnS, pa3MHOKHBIUHXCH B ΟόθΗΧ ΟρββθΧ.

OdopynoBanne, npejyraraeMoe ΗβοόρβτβΗΗβΜ, coctoht H3 οηοτθμηΒ3ΗΤΚΗ KpOBH Η ΟΗΟΤβΜΗ Mff reMOKyJIBTypíí. ΟΗΟΤβΜΗ JVIH ΓβΜΟΚγΛΒ-TypH coctoht H3 οτθκΕΗΗΗΟΓο Kopnyca H3 SByx nacTeS, cocyna JVIHreMOKyjiiTypa Γη 2, npescTasjuromero codoS awnyjiy, cocTomnyi) H3nacTH I h nacTH. 2. 7 ©τογο cocyna I h 2 ημθθτοη KQJimeoópa3HoecyseHHe 3, pa3nejraionjee cocyn B&amp;nHHy b οπρβββΛβΗΗΟΜΟΤΗόπιβΗΗΗno od-Beiy 1:3.OdopynoBanne, npejyraraeMoe ΗβοόρβτβΗΗβΜ, coctoht H3 οηοτθμηΒ3ΗΤΚΗ KpOBH Η ΜΗβΜΗ Mff reMOKyJIBTypíí. ΟΗΟΤβΜΗ JVIH TypβΜΟΚγΛΒ-TypeH coctoht H3 οτθκΕΗΗΗΟΓο Kopnyca H3 SByx nacTeS, cocyna JVIHreMOKyjiiTypa Γη 2, npescTasjuromero codoS awnyjiy, cocTomnyi) H3nacTH I h nacTH. 2. 7 © cocyna I h 2 KQJimeoópa3HoecyseHHe 3, pa3nejraionjee cocyn B & nHHy b οπρβββΛβΗΗΟΜΟΤΗόπιβΗΗΗno 1: 3.

Ha noBepxHocTH KCwrmeodpa3Horo cysefínn 3 no odBéwy pacnonoseHOneprvíafíeETHoe Koňmo 9, odecnenHBajoiqee, ánaronapn BHyTpeHHewyHanpnseEHE) b cTemre, de3onacHoe, ήηοτοθ h npocToe pasnejienneodenx nacTeS cocyna c retóóicynBTypoá I h 2. JlnaLieTp KOJimeod-pa3Horo cysemin 3 3θβηοητ ot nzaMeTpa nponycKHoro οτΒβροτηηjyin npnEHBOHHoS ne τjih h paBeH npndJi. 6 10 mm. ' _Ha noBepxHocTH KCwrmeodpa3Horo cysefínn 3 NO odBéwy pacnonoseHOneprvíafíeETHoe Koňmo 9 odecnenHBajoiqee, ánaronapn BHyTpeHHewyHanpnseEHE) b cTemre, de3onacHoe, ήηοτοθ h npocToe pasnejienneodenx nacTeS cocyna c i h retóóicynBTypoá second JlnaLieTp KOJimeod pa3Horo cysemin 3-ol 3θβηοητ nzaMeTpa nponycKHoro οτΒβροτηηjyin npnEHBOHHoS not τjih h paBeH npndJi . 6 10 mm. '_

. ΕπΕΚβΗ e TBepnan ΠΗτβτοηΒΗΗβ ~ cpenn pacnonoKeHH b cocyne jyin'reMOKyjE&amp;TypH I; 2 ηοοηβηοΒΗτοηΒΗΟ h conpzKacaioTCH npyr c npy-γομ τθμ, hto B nacTH cocyna jyra reMOKyjiBTypn I; 2 conepsHTCH τΒθρηπϋ arap, a b nacTH I - JKHEKaa γηπήτθλβηοη cpena II. Ejia-ronapn KOJn>neoópa3HOMy cy^emno 3 φπκοΗργθτοη odfcéM h noJiose-HHe TBepnoro arapa τθμ, ήτο oh ysepsumaeTca η3γη6ομ cyzeHiiH.OdseM, a τθμ cai.MM h pa3wep cocysa jvih reMOKyjiLTypa I; 2BapLHpyiOTCH B ΟΟΟΤΒΘΤΟΤΒΗΗ O B3HMaeMHM KQTIHHGCTBOM KpOBH. EJia-rcináps co^eTarano HHTaTeJiLHHX cpen c οπρθαθλθηηημη aHTHdaKTepn-UHAHHMH flOdaBKaMH ΠΟ ΟΤΗΟΙϋβΗΗΚ) K SaiUHTHHM ΒΘΙΠΘ0ΤΒ3Μ TeJiaCOrJiaCHO Η300ρθΤΘΗΗΚ) CO3JíaeTCH B03M0JSH0CTB dpaTB jpifl aHaoBaMeHLmee κοληήθοτβο kpobhh τθμ οθμημ coxpaHHTB waraS pa3Mep hoóbgm cocynoB I h 2, t.k. y&amp;e HedcuiBinoe κοπηήθοτβο . Mimpoopra-hh3mob jvih BHpamHBaHHa b reMOKyjiBType naeT 3aMeTHy0 peaKióao.. ΕπΕΚβΗ e TBepnan ΠΗτβτοηΒΗΗβ ~ cpenn pacnonoKeHH b cocyne jyin'reMOKyjE & TypeH I; 2 ηοοηβηοΒΗτοηΒΗΟ h conpzKacaioTCH npyr c npy-γομ τθμ, hto B nacTH cocyna jyra reMOKyjiBTypn I; 2 conepsHTCH τΒθρηπϋ arap, and b nacTH I - JKHEKaa γηπήτθλβηοη cpena II. Ejia-ronapn KOJn> neoópa3HOMy cy ^ emno 3 φποΗργθτοη odfcéM h noJiose-HHe TBepnoro arapa τθμ, για oh ysepsumaeTca η3γη6ομ cyzeHiiH.OdseM, and τθμ cai.MM h pa3wep cocysa jvih reMOKyjiLTypa I; 2BapLHpyiOTCH B ΟΟΟΤΒΘΤΟΤΒΗΗ B3HMaeMHM KQTIHHGCTBOM KpOBH. EJia-rcináps what ^ eTarano HHTaTeJiLHHX cPen c οπρθαθλθηηημη aHTHdaKTepn-UHAHHMH flOdaBKaMH ΠΟ ΟΤΗΟΙϋβΗΗΚ) To SaiUHTHHM ΒΘΙΠΘ0ΤΒ3Μ TeJiaCOrJiaCHO Η300ρθΤΘΗΗΚ) CO3JíaeTCH B03M0JSH0CTB dpaTB jpifl aHaoBaMeHLmee κοληήθοτβο kpobhh τθμ οθμημ coxpaHHTB waraS pa3Mep hoóbgm cocynoB i h 2, T.K. y &amp; e HedcuiBinoe κοπηήθοτβο. Mimpoopra-hh3mob jvih BHpamHBaHHa b reMOKyjiBType naeT 3aMeTHy0 peaKióao.

Cocya jyin reMOKyjiLTypH I; 2 npeuMyiuecTBeHHO npencTasjínoT co-dofí aivmyjiy c odJiaMHBaeMofi οτθκλήηηοη BepxyuiKofi 4, oKpyseHHoS ' .azracTiraHHM οοθληηητθλβηημ MaTepnajroM 5, Hanp. TpydoHKoS H3npo3paHHofí xwacTMacoH mm ΠΒΧ Tuna“60 s kL«.r- ” t3khm odpa3OM cwc-Tewa &amp;jlh reMOKyjiLTypu οοθηηηηθτοη c cHCTewofi b3hthh kpobh hnpn nocjienoBaTeJiLHOM pacnoJioKeraiH npencTaBJUieT codoS θληηοθ .nejioe.Cocya jyin reMOKyjiLTypH I; 2 npeuMyiuecTBeHHO npencTasjínoT co-dofí aivmyjiy c byJiaMHBaeMofi BepxyuiKofi 4, oKpyseHHoS '.azracTiraHHM οοθληηητθλβηημ MaTepnajroM 5, Hanp. TpydoHKoS H3npo3paHHofi xwacTMacoH mm ΠΒΧ Tuna “60 s kL« .r- ”t3khm odpa3OM cwc-Tewa &amp;

Diaronapn sJíacTHHHOH οοθαηηητθλβηοη TpydonKe 5 ocb οηοτθμηb3hthh κροΒΗ pacnojraraeTcn no# yrzioM κ och οηοτθμη jmih γθμο-KyjiLTypH h ee mosho ποβορ&amp;ηηβθτβ b jtoóom HanpaBJíeHHH. CaoTeMaB3HTHH KpOBH COCTOHT H3 ΕΗΒΘΚϋΗΟΗΗΟΗ HTJIH 6, 3aKJHOHeRHOS B .CT.eKJWHHyu odúJioHKy7H ynJLHHeHHyio BHH3y tsk, hto što yzyiHHeHHeoxBaTHBaeT odJiaMHBaeMyio Bepxyumy 4 cocyna jpra reMOKyjn>TypHI; 2. OdJiaMHBaeMaH BepxymKa 4* cocyna jyra reMOKyjiBTypH I; 2,sJíacTHHHaji coeřCHHHreJiBHaH TpydoHKa 5 h οηοτθμη B3hthh kpobhoKpyjsenH κοππηηκομ 8.Ar TH TH H OH OH OH ο py py py py py py py py py py py py py py py py py py pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa pa CaOTeMaB3HTHH KpOBH COCTOHT H3 ΕΗΒΘΚϋΗΟΗΗΟΗ HTJIH 6, 3 JKJHOHeRHOS B .CT. 2. OdJiaMHBaeMaH BepxymKa 4 * cocyna jyra reMOKyjiBTypH I; 2, sJíacTHHHaji coeřCHHHreJiBHaH TpydoHKa 5 h οηοτθμη B3hthh kpobhoKpyjsenH κοππηηκομ 8.

IIpocTpaHCTBO 12 HaxofíHTcn non BaicyyMOM hhh BHpainHBaHHHcnenHaJE&amp;HHx KyjiBTyp daKTepnS 3ηπολήθήο ra30M c onpeneneHHHMnasjíeHHeM. JUh npoKajiHBaHHH βθηη nepen πολβ3οβ3ηηθμ o. npHcno—codjieHHH cHHMaeTCH KOJinanoK 8, οτθκιιηηηθη odoJioHKa 7 htjih 6Ha£pe3aeTca h odjraíMBaeTcn tsk, hto ooBodoamaeTcn CTepiuiLHanin?jra 6. Ilocjie BKajncBaHHS b-Befly npoH3BQHHT.CH cpesaimnee λβπ&amp;θ-ΗΗΘ ΟΗΟΤΘΜΗ ftTLH reMOKyJIBTypH BOKpyr OCH; ΟΗΟΤΘΜΗ B3HTHH κροΒΗ,noica cTeKTuamaa Bepxynma^He odJioMaeTca; zrera 6 οοτηθτοη ηθπολ-ehshoS. Ποβ B03fleScTBHeM BaxyyMa b HTJie 6 co3naeTcn ηοηηκθη-ΗΟΘ ΒΘΒΠΘΗΗΘ, Tas ΗΤΟ B ΟΟΟΤΒΘΤΟΤΒΗΗ O ΗΜΘΕϋίΗΜΟΗ BaKyyMOM l - II - onpejieJieHHoe kojihhcctbo kpobh aBTOMáTinecKH oTcacHBaeTcn bcocyn I; 2 οηοτθμη bshthh kpobh. ΟβΗΟΒρβΜθΗΗΟ C THKHM OTČaCHBaHHÓM KpOBH B fl03Hp0BaHH.0M KOJIH-ηθοτβθ προποχοβΗΤ BHOKyjumHíi khhko0 cpe^H II. h CMemaHHoS. c HeSTBepnoĚ arapoBoň $a3H 10. - ' Ποαπό yjjajieHHH ηηβθκηηοηηοη htjih 6 H3 βθηη η 33Κρητηη ee οτθ-pmitHOH προόκοδ, npeuMyiqecTBeHHo H3. miacTMáccH, προΒΟβΗτοη πρπonpejiejieHHOM ηθιμοηηομ ποεοχθηηη βοθ® οηοτθμη rjix reMOKyjiLTypnHHKyóamiH miTaTeJiLHoS cpejtH 10 η II npn TeMnepaType, npero.iy-mecTBeHHo b íEana3Ofíe 35-37° C.IIpocTpaHCTBO 12 HaxofiHTcn non BaicyyMOM hhh BHpainHBaHHHcnenHaJE &amp; HHx KyjiBType daKTepnS 3ηπολήθήο ra30M c onpeneneHHHMnasjíeHHeM. South npoKajiHBaHHH βθηη Nepen πολβ3οβ3ηηθμ on. NpHcno-codjieHHH cHHMaeTCH KOJinanoK 8 οτθκιιηηηθη odoJioHKa 7 htjih 6HA £ h pe3aeTca odjraíMBaeTcn TSK HTO ooBodoamaeTcn CTepiuiLHanin? JRA sixth Ilocjie BKajncBaHHS b-Befly npoH3BQHHT.CH cpesaimnee λβπ &amp; θ-ΗΗΘ ΟΗΟΤΘΜΗ ftTLH reMOKyJIBTypH BOKpyr OCH; 3 B3HTHH κροΒΗ, noica cTeKTuamaa Bepxynma ^ He by JioMaeTca; zrera 6 οοτηθτοη ηθπολ-ehshoS. Π β ο 03 03 03 03 6 ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ 6 ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ K K K ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ ΘΗΗΘ 2 οηοτθμη bshthh kpobh. ΟβΗΟΒρβΜθΗΗΟ C THKHM OTČaCHBaHHOM KpOBH B fl03Hp0BaHH.0M KOJIH-ηθοτβθ προποχοβΗΤ BHOKyjumHii khhko0 cpe ^ H II. h CMemaHHoS. c HeSTBepnoe arapoBoň $ a3H 10. - 'H α y j hHH ηηβθκηηοηηη htjih 6 H3 θθηη η 33ρητηη ee οτθ-pmitHOH προόκοδ, npeuMyiqecTBeHHo H3. ac ac 10 j 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10-----

OnpefleJieHHoe ηηκτοηηοθ ποτιοκθηηθ cocyna I; 2 οηοτθμη λλη rewo-OnpefleJieHHoe ηηκτοηηο ποτιοκθηηθ cocyna I; 2 οηοτθμη λλη rewo-

- KyjiBTypa TpeóyeTca b 33Βηοημοοτη ot odtewa cocyna, a τθμ čaMHMη ΤΒΘρβοιι mm KiiflKOH πητθτθλβηοΗ cpenn 10; II nm τογο, HTodH ' iuieHKa keekoE rmTaTexbHoa cpenn II ηθοτηηηο noKpHBana noBepx-hoctb TBepnoro arapa.IO. B CBH3H c HHKydaimeíi ιηπήτθλβηοη cpesu ΙΟ; II προΗΟχοβΗΤ pa3-μηοηθηηθ daKTepná, ροοτ Ky&amp;LTyp daKTepirii b odezx cpenax cTa-HOBHTCH SaMeTHHM, COímaCHO H30dpeTGHHH), ΠΟ Η3ΜΘΗΘΗΗΚ) ΙΧΒΘΤΘΟ,ζΗΟΓΟ H3 3ΤΗΧ flOdaBJIGHHHX HHAHKaTOpOB.- KyjiBTypa TpeóyeTca b 33Βηοημοοη ot ottete cocyna, and τθμ čaMHMη ΤΒΘρβοιι mm KiiflKOH πητθτθλβηοΗ cpenn 10; II nm τογο, HTodH 'iuieHKa keekoE rmTaTexbHoa cpenn II ηθοτηηηο noKpHBana noBepx-hoctb TBepnoro arapa.IO. B CBH3H c HHKydaimei ιηπήτθλβηοη cpes ΙΟ; Π 3 ΗΤ 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3.

IvtopodHOjrormiecKoe HccJiesoBajme προΒοβΗτοη οτβ&amp;ΖΓΒΗΟ nyTeM pa3-£ΘΛΘΗΗΗ ΟΗΟΤΘΜΗ B3HTHS KpOBH H ΟΗΟΤΘΜΗ C reMOKyJlLTypOÉ HOCJieHanpe3aHEH h oTJiaMHBaHHH nacTH I h 2 cocyna I; 2 y KOJima 9cyseimn 3. ' B CBH3H C 9THM ΠρΟΗΟΧΟβΗΤ ΟΤΛΘΛΘΗΗΘ KH£KOH θρθ£Η II OT ΤΒΘ£«ΟΪΙ ,cpenn, odpocmefí daKTepHHMH 10, τηκ ήτο οτθηοβητοη βοθμοηηημpa3seJii)HHfi saceB η β3Ητηθ aHannsoB sjih naHBHeSnieH.daKTepHOJio-rmiecKoS odpadoTKH mm HccjresoBaHEH odenx cpefl. ΟτββΛβΗΗβ jkhhkoS ηητθτθλβηοη cpenn II ot TBepnofi arapoBoii $a-3H 10 npoE3EojuiTcn πρΗ οτλομθ cocyna I; 2 οηοτθμη B3sthh κρο-bh TaK, hto nacTL 2 c arapoM Hanpamiena BBepx. Bjiaronapn θτο-My cocyn I; 2 mo&amp;ho. 3anojiHHTB onpeneJieHHHM ra30M, b κοτοροΜMoryT pa3MHosaTLCH daKTepra, TpynHo odHapysusaeRiHe b κποΛοροη-ho0 aTMocčepe.T 3 j 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3;;;;;;;;;;;;;;;;; 2 y Kojima 9cyseimn third 'B CBH3H C 9THM ΠρΟΗΟΧΟβΗΤ ΟΤΛΘΛΘΗΗΘ KH KOH θρθ £ £ Η II OT ΤΒΘ £ «ΟΪΙ, cpenn, odpocmefí daKTepHHMH 10 τηκ ήτο οτθηοβητοη βοθμοηηημpa3seJii) HHfi saceB η β3Ητηθ aHannsoB sjih naHBHeSnieH.daKTepHOJio-rmiecKoS odpadoTKH mm HccjresoBaHEH odenx cpefl. ΟτββΛβΗΗβ jkhhkoS ηητθτθλβηοη cpenn II ot TBepnofi arapoBoii $ a-3H 10 npoE3EojuiTcn πρΗ οτλομθ cocyna I; 2 B3sthh κρο-bh TaK, hto nacTL 2 c arapoM Hanpamiena BBepx. Bjiaronapn-My cocyn I; 2 mo &amp; ho. 3anojiHHTB onpeneJieHHHM ra30M, b κοτοροΜMoryT pa3MHosaTLCH daKTepra, TpynHo odHapysusaeRiHe b κποΛοροη-ho0 aTMocčepe.

Φο pMyjra - H3 od ροτθηηη 1. MeTOfl ΒΗΗΒΛβΗΗίϊ tíaitTepHMHK, npž KOTOPOM KPOBB OTCaCHBaeTCHB AO3HpOBaHHOM KOJBWeCTBě ΗΘρΘ3 CH0T6My B3HTHH KpOBH B CO-cyA πόα BaicyyMOM h/oh sanoJiHeHHHS cneiwr&amp;HHM ’ra30M jyiflreMOKyuiBTyp η npn : stom cMenumaeTcn c wckoíí πητητθλβηοΑcpeftoS, oTjnnanimHCH τθμ, hto kpobb, oTcocaHiian b cocys ;oAHOBpeMeHHo HHOKyjiHpyeí wtKyio nnraTeJiBHyip cpejiy caHTH-.daKTepniTOHHi^iH AOóaBKaMH η, ομθπιθβπιποβ c HeS, - TBepnyu πη-TaTěJiLHyio cpeAy, η τθμ, ψγο ποοαθ βτογο πρκ onpeAeJieHHoSTeMnepaType h HamioHHOM ποαοηθηηη cocyaa b odenx cpeaax bh-padaTHBaeTcn KyjiLTypa daiíTepnS η οτηηοβητοη 38Μθτηο3 dJiaro-Aapn AodaBKe HHOTKaTopa-KpacHTeAff. 2. OdopynoBaHne jvih ηοοηθαοβηηηη tíaKTepHMH0, cocTonmee H3 chc-ΤΘΜΗ B3HTHH KpOBH, H3 ΗΗΒΘΚΙΧΗΟΗΗΟΗ UTAH CO CTeKJTHHHOS OdO-JIOHKOS H OTA6JIBHO0, CBOdOAHO ΟΟΘ£ΗΗΗΚ)ΐηΘΗΟίϊ Ο,ΟΗΟΤΘΜΟβ B3H-THH- KPOBH, ΟΗΟΤΘΜΗ ÍVW reMOKyJILTypH, COCTOHIUeS H3 3anaffH-Horo cocy^a c khakok HHTaTeji&amp;Hot cpeaoS, oTjnreaioineecn τθμ,hto sanaHHHHfi cocys οηοτθμη ahh reMOKyjiLTypn (I; 2) npeA-cTaBJíHéT codoS aimyjiy c K0Jimeodpa3HHM cysemieM (3), a Hase .KQ3imeodpa3Horo cy^eHHs (3),stom $MKCHpoEaHo/fíáxoAHTCH TBepnasnHTaTeJiLHas cpeaa (10) h BHme Koini»neodpa3Horo cysenus (3)HaxoAHTCH wCKan nnraTejiLHaH cpe^a (II), β κολήήθοτβθηηομοοοτποπιθηπη paBHOM npeHMyigecTBeHHO 1:3. 3. OdopyAOBaHne no n. 2, oTJuraammeecH τθμ, hto Ha ποβθρχηοοτηKOdn>neodpa3HoiO cysemoi (3) ημθθτοη kohbiío λομκη (9), hAnaMeTp ero nponycKHoro οτβθροτηη οοοτθβ^ιηθτ 6 — 10 mm, h ero πολοκθηηθ baojib jvihhh ai.myjm CoóTBeTCTByeT κοεηηθοτ-BeHHowy οτηοιιιθηιπο ŤBepnofi ΠΗΤβΤΘΑΒΗοβ čpeAH κ swoS (.10; II). 4. OdopysoBaHne no n.2, oTJnrcaionieecH τθμ, ητο cocya οηοτθμηPJifi ΓΘΜΟΚΥΛΒΤΥΡΗ ΟΟΘβΙΪΗΘΗ C CIICTeMOfi E3HTI1H KpOBH ΉθρΘ3anacTUHflyio TpydoHry (οοθαηηητθλβη^. ήηοτβ) (5) , npeiiMy-mecTEeHHo H3 npo3paHHoíí mhtkoh mracTtiaccH, κθκ 3aKpHToe / B 60 ΠΟΗΟΚΘΉΗΙΙ ποωίθίζΟΒΗΤ&amp;πΒΗοβ eflHHoě neaoe, η ocl οηοτθμη b3htjw κροεζpacncvrojKena πολ yrJioM κ ocii οηοτθμη jym reMOKyjrLTypn η ee'ΜΟ3ΚΗΟ nOBOpa^HBáTL B JWÓOM H3JipaBJI6HKK. . . 5» OdopynoBamie no n. 2, oTJinnaroineeca τθγλ, hto odiaMHBaeMHfi- οτθκζΐΗΗΗΗΐΙ κοΗθη (4) cocy^a οηοτθΜΗ &amp;tih reMOKyjr&amp;TypH (I; 2) pacnouio&amp;eH b hjdkhoS nacTH eTeKJLHHHofi Tpydomcz (7),oxBaneHHoS oJiacTiraíoS οοθληηητθλβηοζ TpyÓKofi (5). 6. OdopysoBamie no n.. 2, oTjnraawecfl τθμ, hto. saacTiniHaa .coeAHHHTeJiLHaH nacTL (5), KHBeKnnoHHaa mma (6) z οτθκίηη-Haa TpydoHKa (7) HazpuTH κολπβηκομ (8).Φο pMyjra - H3 from ροτθηηη first MeTOfl ΒΗΗΒΛβΗΗίϊ tíaitTepHMHK, NPZ KOTOPOM KPOBB OTCaCHBaeTCHB AO3HpOBaHHOM KOJBWeCTBě ΗΘρΘ3 CH0T6My B3HTHH KpOBH B CO-cya πόα BaicyyMOM H / OH sanoJiHeHHHS cneiwr &amp; HHM 'ra30M jyiflreMOKyuiBTyp η npn: one hundred meters cMenumaeTcn c wckoíí πητητθλβηοΑcpeftoS, oTjnnanimHCH τθμ, hto kpobb, oTcocaHiian b cocys; oAHOBpeMeHHo HHOKyjiHpyeí wtKyio nnraTeJiBHyip cpejiy caHTH .daKTepniTOHHi ^ H-AOóaBKaMH η, c ομθπιθβπιποβ HES - TBepnyu πη-TaTěJiLHyio CPEA, η τθμ, ψγο ποοαθ βτογο πρκ onpeAeJieHHoSTeMnepaType h HamioHHOM ποαοηθηηη cocyaa b odenx cpeaax BH-padaTHBaeTcn KyjiLTypa daíTepnS η οτηηοβητοη 38Μθτηο3 dJiaro-Aapn AodaBKe HHOTKaTopa-KpacHTeAff. Second OdopynoBaHne jvih ηοοηθαοβηηηη tíaKTepHMH0, cocTonmee CHC-H3 ΤΘΜΗ B3HTHH KpOBH H3 ΗΗΒΘΚΙΧΗΟΗΗΟΗ UTAH CTeKJTHHHOS CO ODO-H JIOHKOS OTA6JIBHO0, CBOdOAHO ΟΟΘ £ ΗΗΗΚ) ΐηΘΗΟίϊ Ο, ΟΗΟΤΘΜΟβ B3H-THH- KPOBH, ΟΗΟΤΘΜΗ IVw reMOKyJILTypH, COCTOHIUeS H3 3anaffH-Horo Coca-c khakok HHTaTeji &amp; Hot cpeaoS, oTjnreaioineecn τθμ, HTO sanaHHHHfi cocys οηοτθμη AHH reMOKyjiLTypn (I, 2) npeA-cTaBJíHéT codoS aimyjiy c K0Jimeodpa3HHM cysemieM (3) and Hase .KQ3imeodpa3Horo cy ^ eHHs (3), one hundred meters $ MKCHpoEaHo / fíáxoAHTCH TBepnasnHTaTeJiLHas cpeaa (10) h BHme Koini »do not respondHoro cysenus (3) HaxoAHTCH wCKan nnraTejiLHaH cpe ^ a (II), β κολήήθοτβοητποπιθηπη pa: 1: 3. Third OdopyAOBaHne no n. 2, oTJuraammeecH τθμ, HTO Ha ποβθρχηοοτηKOdn> neodpa3HoiO cysemoi (3) ημθθτοη kohbiío λομκη (9) hAnaMeTp ero nponycKHoro οτβθροτηη οοοτθβ ^ ιηθτ 6-10 mm, h ero πολοκθηηθ baojib jvihhh ai.myjm CoóTBeTCTByeT κοεηηθοτ- BeHHowy οτηοιιιθηιπο epBepnofi ΠΗΤβΤΘΑΒΗοβ čpeAH κ swoS (.10; II). Fourth OdopysoBaHne well n.2, oTJnrcaionieecH τθμ, ητο Coca οηοτθμηPJifi ΓΘΜΟΚΥΛΒΤΥΡΗ ΟΟΘβΙΪΗΘΗ C CIICTeMOfi E3HTI1H KpOBH ΉθρΘ3anacTUHflyio TpydoHry (οοθαηηητθλβη ^. Ήηοτβ) (5) npeiiMy-H3 mecTEeHHo npo3paHHoíí mhtkoh mracTtiaccH, κθκ 3aKpHToe / B 60 ΠΟΗΟΚΘΉΗΙΙ ποωίθίζΟΒΗΤ &amp; πΒΗοβ eflHHoě Neaoe, η ocl οηοτθμη b3htjw κροεζpacncvrojKena πολ yrJioM κ ocii οηοτθμη jym reMOKyjrLTypn η ee'ΜΟ3ΚΗΟ nOBOpa ^ HBáTL B JWÓOM H3JipaBJI6HKK. . . 5 »OdopynoBamie no n. 2, oTJinnaroineeca τθγλ, HTO odiaMHBaeMHfi- οτθκζΐΗΗΗΗΐΙ κοΗθη (4) Coca-and οηοτθΜΗ &amp; TIH reMOKyjr &amp; typhi (I, 2) pacnouio &amp; eH b hjdkhoS nacTH eTeKJLHHHofi Tpydomcz (7) oxBaneHHoS oJiacTiraíoS οοθληηητθλβηοζ TpyÓKofi (5). 6. OdopysoBamie no n. 2, oTjnraawecfl τθμ, hto. saacTiniHaa .coeAHHHTeJiLHaH nacTL (5), KHBeKnnoHHaa mma (6) from οτθκίηη-Haa TpydoHKa (7) HazpuTH κολπβηκομ (8).

AHHQTawAHHQTaw

IfeoópeTeHHe KacaeTcn μθτολη h odopynoBaHna jym βηηβηθηηη tíaK-ΤβρΗΜΗβ nyTeM ΠρΗΜΟΓΟ B3HTHH KpOBH H KyJIBTHBHpOBaHHH reMOKyJIB-Typ. . · .IfeoópeTeHHe KacaeTcn μθτολη h odopynoBaHna jym βηηβηθηη tíaK-ΤβρΗΜΗβ nyTeM ΠρΗΜΟΓΟ B3HTHH KpOBH H KyJIBTHBHpOBaHHH reMOKyJIB-Typ. . ·.

IfejIB» Η3Ο0ρθΤβΗΗΗ ΗΒΛΗΘΤΟΗ ynpoqeHHe ΜΘΤΟ£ά Β3ΗΤΗΗ KpOBH Η. reMOKyjiBTyp η υμθηβπιθιιηθ 3aTpaT Ha annapaŤypy. Β OCHGBy Κ3Ο($ρθΤΘΗΙΙΗ HOJTOJKeHa sahana 003Λ3ΤΒ μθτο#, πο κοτορο- . My coKpainaioTOH Bpew h nyTB,. nannHan c μομθητα ββητηη κροΒΗ soB03£efíCTBHH Ha 3ΚΗ£ΚΗΘ H TBepUHe HHTaTeJILHHe KyJlBTypH C KpOBBDh co3flaHHH oóopyáoBáHiíff c ΜβΗΒΐιπίΜ kooHecTBOM neTajien. KpOBB, ' OTCOCaHHaa B ,HO3HpOBaHH0M ΚΟΛΗΗΘΟΤΒΘ CHCTeMOS B3HTHH KpOBH Bcocy#,- HHOKyjiHpyeT οεηοβρθμθηηο aujncyio h TBepnyio miTaTejiBHyBcpeny h OTHMyjrHpyeT b hhx KyjiBTypy daKTepnít, KOTopan cTaHOBHTcabhahoh djiaro^apn HHAHKaTopy-KpacHTejno. y CHCTeMH flJLH reMOKýJIBTypH ΗΜΘ6Τ0Η COCyH B BW aMnyjm G KOJIB-rteodpa3HHM cyseHHeM. ÍIojroseHHe K0JiBixeodpa3Horo cyjKeHHH npncno-co&amp;ieHo no jyiHHe aMnyjm κ HeodxoaHMOMy οοοτηοπιθηκη) KQjnraecTBaTBépnoS H jkhjucoS nHTaTejiBHOH cpenu, npeHMymecTBeHHO κ oŤHorne-hhh) 1:3. Ode nHTaTeJiBfíHe cpe#n pacnoJioHeHH nocjreflOBaTejiBHO h-3a$HKCHpOBaHH Β ΟΒΟΘΜ HOJIOSeHHH.IfejIB »Ο3Ο0ρθΤβΗΗΗ n ynpoqeHHe ΜΘΤΟ £ ά Β3ΗΤΗΗ KpOBH Η. reMOKyjiBTyp η υμθηβπιθιιηθ 3aTpaT Ha annapaŤypy. Β OCHGBy Κ3Ο ($ ρθΤΘΗΙΙΗ HOJTOJKeHa Sahana 003Λ3ΤΒ μθτο #, πο κοτορο-. We coKpainaioTOH Bpew h ,. nyTB nannHan c μομθητα ββητηη κροΒΗ soB03 efíCTBHH Ha £ £ 3ΚΗ ΚΗΘ H TBepUHe HHTaTeJILHHe KyJlBTypH C KpOBBDh co3flaHHH oóopyáoBáHiíff c ΜβΗΒΐιπίΜ kooHecTBOM neTajien. KpOBB, 'OTCOCaHHaa b HO3HpOBaHH0M ΚΟΛΗΗΘΟΤΒΘ CHCTeMOS B3HTHH KpOBH bcoc # - HHOKyjiHpyeT οεηοβρθμθηηο aujncyio h TBepnyio miTaTejiBHyBcpeny h OTHMyjrHpyeT b HHX KyjiBTypy daKTepnít, KOTopan cTaHOBHTcabhahoh djiaro-APN HHAHKaTopy-KpacHTejno. y CHCTeMH flJLH reMOKýJIBTypH ΗΜΘ6Τ0Η COCyH b BW aMnyjm G KOJIB-rteodpa3HHM cyseHHeM. I oj ose 0 0 0 cy cy cy cy cy cy cy I I I I I I I y oro oro oro oro)) p p p p p p p:::::: 1 1 De HT e pe pe pe pe---------OJ OJ OJ OJ OJ OJ OJ HOJIOSeHHH.

Ha φπτ. X npe^cTaBJíeHo. odopynoBamie, npe&amp;JiaraeMoe Η3θόρβτβΗΗθΜ.Ha φπτ. X is not. odopnoBamie, npe &amp; JiaraeMoe Η3θόρβτβΗΗθΜ.

Claims (6)

9 Předmět vynálezu9 Object of the Invention 1. Způsob zjišťování bakterií, při kterém se krev odsáváve stanoveném množství systémem pro odběr krve do nádobkypod vakuem a/nebo naplněné speciálním plynem pro hemokul-turu a při tom se mísí s kapalným živným mediem, vyznačující setím, že krev odsávaná do nádobky současně inokuluje kapalnéživné medium s antibaktericidními přídavnými látkami a přisměšování a ním pevné živné medium a pak se při určité teplotěa naklonění nádobky kultivuje v obou mediích kultura bakterií,která se stává viditelnou přídavkem baryiva-indikátoru.What is claimed is: 1. A method for detecting bacteria wherein the blood is aspirated at a determined amount by a blood collection system under a vacuum and / or filled with a special hemoglobin gas, and mixed with a liquid nutrient medium, wherein the blood is aspirated into the container at the same time. the liquid medium with antibacteric additives and the mixing and solid nutrient medium thereof, and then, at a certain temperature of the container tilting, a culture of bacteria is cultivated in both media, becoming visible by the addition of the barium indicator. 2. Zařízení pro zjišťování bakterií sestávající ze systémuodběru krve, z injekční jehly se skleněným obalem a odděle-ného, volně se systémem odběru krve spojeného systému prohemokulturu, tvořeného zatavenou nádobkou z kapalným živnýmmediem, vyznačující se tím, že zatavenou nádobkou systému prohemokulturu je ampule s kruhovým zúžením (3) a pod kruhovýmzúžením (5) je fiksováno pevné živné medium (10) a nad zúžením(3) se nachází kapalné živné medium (11), v poměru množství 1,52. A device for detecting bacteria consisting of a blood collection system, a glass container injection needle and a detached, free blood collection system of a combined democulture system consisting of a sealed container of liquid nutrient medium, characterized in that the sealed container of the prohemoculture system is an ampoule by a circular constriction (3) and under a circular constriction (5), a solid nutrient medium (10) is fried and a liquid nutrient medium (11) is located above the constriction (3), at a ratio of 1.5 .3· Zařízení podle bodu 2, vyznačující se tím, že na povrchukruhového zúžení (3) je kroužek (9) lomu a průměr jehoprostupního otvoru činí 6 - 10 mm a jeho umístění na délceampule odpovídá poměru množství pevného živného mediá (10)ke kapalnému živnému mediu (11).3. Apparatus according to claim 2, characterized in that the surface ring ring (3) has a refraction ring (9) and the diameter of the aperture is 6-10 mm, and its position on the long-stem corresponds to the ratio of the solid nutrient medium (10) to the liquid nutrient medium (11). 4. Zařízení podle bodu 2, vyznačující se tím, že nádobkasystému pro homokulturu je sp'o je na se systémem odběru krveelastickou částí (5) z průhledného měkkého materálu z plasti-cké hmoty, jako uzavřené za sebou následující jednotné tělesoa osa systému odběru krve je umístěna pod úhlem k ose systémupro hemokulturu a je možno ji otáčet v libovolném směru·4. Apparatus according to claim 2, characterized in that the system for the homoculture is directly connected to the blood-elastic part (5) of the transparent soft material of the plastic material, as the closed unitary body of the blood collection system. it is placed at an angle to the axis of the system for the blood culture and can be rotated in any direction · 5. Zařízení podle bodu 2, vyznačující se tím, že olemovanáskleněná špička (4) nádobky systému pro hemokulturu je umístěnave spodní části skleněné trubičky (7)» obklopené elastickouspojovací částí (5)·5. Apparatus according to claim 2, characterized in that the hemmed glass tip (4) of the hemoculture system container is positioned at the bottom of the glass tube (7) surrounded by the elastic-bonding portion (5). 6· Zařízení podle bodu 2, vyznačující se tím, že elastickáspojovací část (5), injekční jehla (6) a skleněná trubička (7)jsou zakryty krytem (8) Uznáno vynálezem na základě výsledku expertizy, provedenéÚřadem pro vynalezectví a patentnictví, Berlín, DD 1 výkres6. Apparatus according to claim 2, characterized in that the elastic connection portion (5), the injection needle (6) and the glass tube (7) are covered by a cover (8) recognized by the invention based on the results of an expert review by the Office of Inventing and Patenting, Berlin. DD 1 drawing
CS376180A 1979-06-28 1980-05-28 Method of detection of bacteria and equipment to perform this method CS230958B1 (en)

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US3692486A (en) * 1971-07-12 1972-09-19 Cybertek Inc Methods and apparatus for obtaining the quantitation and the concentrations of precipitin reactions and participating molecules in biological fluids
US3817240A (en) * 1972-06-28 1974-06-18 Becton Dickinson Co Multiple sample needle assembly with one-way valve and blood flow indicator
US4026767A (en) * 1975-11-19 1977-05-31 Wisconsin Alumni Research Foundation Test procedure for microorganisms in blood
GB1601689A (en) * 1977-07-15 1981-11-04 Mariel C Method of diagnosing bacteremia and apparatus therefor
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ATA174680A (en) 1981-09-15
GB2054144B (en) 1984-02-15
GB2054144A (en) 1981-02-11
ES8105928A1 (en) 1981-05-16
AT366713B (en) 1982-05-10
ES492885A0 (en) 1981-05-16
BG34473A1 (en) 1983-10-15
DD149843A3 (en) 1981-08-05
FI801166A (en) 1980-12-29
SU1440925A1 (en) 1988-11-30
FI70045B (en) 1986-01-31
FI70045C (en) 1986-09-12
SE8002556L (en) 1980-12-29
SE448737B (en) 1987-03-16
FR2460332A1 (en) 1981-01-23
FR2460332B1 (en) 1984-12-07
CH652295A5 (en) 1985-11-15

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