CO5721022A2 - Metodo de determinacion de agrupamientos especificos que constituyen las heparinas - Google Patents
Metodo de determinacion de agrupamientos especificos que constituyen las heparinasInfo
- Publication number
- CO5721022A2 CO5721022A2 CO05025348A CO05025348A CO5721022A2 CO 5721022 A2 CO5721022 A2 CO 5721022A2 CO 05025348 A CO05025348 A CO 05025348A CO 05025348 A CO05025348 A CO 05025348A CO 5721022 A2 CO5721022 A2 CO 5721022A2
- Authority
- CO
- Colombia
- Prior art keywords
- heparinase
- depolymerization
- depolymerized
- heparin
- reducing agent
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Materials Engineering (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Saccharide Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
Abstract
1.- Método de analisis de heparinas o de heparinas de bajo peso molecular, caracterizado porque se efect·an las etapas siguientes:Despolimerizacion de la muestra por accion de heparinasas Llegado el caso, reduccion del despolimerizado, y Valoracion por Cromatografia liquida de Alta Eficacia2.- Método seg·n la reivindicacion 1, caracterizado porque se busca la presencia de cadenas oligosacaridas en las que la terminacion esta modificada con un enlace 1,6-anhidro.3.- Método seg·n la reivindicacion 1 o 2, caracterizado porque las heparinasas estan en forma de una mezcla de heparinasa 1 (EC 4.2.2.7.), heparinasa 2 (heparin-liasa II) y heparinasa 3 (EC. 4.2.2.8.).4.- Método seg·n la reivindicacion 1, caracterizado porque la heparina despolimerizada por accion de heparinasa (despolimerizado) se somete luego a un agente de reduccion.5.- Método seg·n la reivindicacion 4, caracterizado porque el agente de reduccion es NaBH4 o una sal de metal alcalino del anion borohidruro.6.- Método seg·n la reivindicacion 1, en el que el método cromatografico empleado es una cromatografia de intercambio de anion.7.- Método seg·n la reivindicacion 6, caracterizado porque se emplea una fase movil transparente en el UV hasta 200 nm.8.- Método seg·n la reivindicacion 6 o 7, caracterizado porque la fase movil empleada es a base de perclorato de sodio, sales de metanosulfonato o sales de fosfato.9.- Método seg·n la reivindicacion 6, caracterizado porque se utiliza un método de deteccion que permite detectar selectivamente los az·cares acetilados.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR0211724A FR2844808B1 (fr) | 2002-09-23 | 2002-09-23 | Methode de determination de groupements specifiques constituant les heparines ou les heparines de bas poids moleculaire |
| US42248202P | 2002-10-31 | 2002-10-31 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO5721022A2 true CO5721022A2 (es) | 2007-01-31 |
Family
ID=32031841
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO05025348A CO5721022A2 (es) | 2002-09-23 | 2005-03-18 | Metodo de determinacion de agrupamientos especificos que constituyen las heparinas |
Country Status (18)
| Country | Link |
|---|---|
| EP (1) | EP1558755B1 (es) |
| JP (1) | JP4373917B2 (es) |
| KR (1) | KR20050057550A (es) |
| CN (1) | CN101747385B (es) |
| AU (1) | AU2003279430B2 (es) |
| BR (1) | BRPI0314654B8 (es) |
| CA (1) | CA2499537A1 (es) |
| CO (1) | CO5721022A2 (es) |
| HK (1) | HK1081601A1 (es) |
| HR (1) | HRP20050273A2 (es) |
| IL (1) | IL167569A (es) |
| MA (1) | MA27393A1 (es) |
| MX (1) | MXPA05001945A (es) |
| NO (1) | NO20051744L (es) |
| PL (1) | PL376709A1 (es) |
| RS (1) | RS20050230A (es) |
| RU (1) | RU2359037C2 (es) |
| WO (1) | WO2004027087A2 (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7575886B2 (en) | 2002-03-11 | 2009-08-18 | Momenta Pharmaceuticals, Inc. | Analysis of sulfated polysaccharides |
| EP1582531A1 (en) * | 2004-03-24 | 2005-10-05 | Aventis Pharma S.A. | Process for oxidizing unfractionated heparins and detecting presence or absence of glycoserine in heparin and heparin products |
| FR2880951B1 (fr) * | 2005-01-19 | 2015-09-18 | Aventis Pharma Sa | Methode d'analyse d'oligosaccharides a partir de plasma sanguin |
| US8101733B1 (en) | 2006-06-27 | 2012-01-24 | Momenta Pharmaceuticals, Inc. | Methods of evaluating mixtures of polysaccharides |
| WO2008068854A1 (ja) | 2006-12-05 | 2008-06-12 | Glycoscience Laboratories, Inc. | 変形性関節症の治療剤 |
| US7968082B1 (en) | 2007-01-26 | 2011-06-28 | Momenta Pharmaceuticals, Inc. | Evaluating mixtures of low molecular weight heparins by NMR |
| US7790466B1 (en) | 2007-01-26 | 2010-09-07 | Momenta Pharmaceuticals, Inc. | Evaluating mixtures of low molecular weight heparins by chain profiles or chain mapping |
| US9139876B1 (en) | 2007-05-03 | 2015-09-22 | Momenta Pharmacueticals, Inc. | Method of analyzing a preparation of a low molecular weight heparin |
| WO2011090948A1 (en) | 2010-01-19 | 2011-07-28 | Momenta Pharmaceuticals, Inc. | Evaluating heparin preparations |
| WO2012115952A1 (en) | 2011-02-21 | 2012-08-30 | Momenta Pharmaceuticals, Inc. | Evaluating heparin preparations |
| CN103755823B (zh) * | 2013-12-12 | 2016-09-28 | 中国海洋大学 | 一种硫酸软骨素中硫酸角质素的纯化与检测方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8102987A (en) * | 1986-10-02 | 1988-04-21 | Massachusetts Institute Of Technology | Neutralizing anticoagulant activities of low molecular weight heparin |
| US6190875B1 (en) * | 1997-09-02 | 2001-02-20 | Insight Strategy & Marketing Ltd. | Method of screening for potential anti-metastatic and anti-inflammatory agents using mammalian heparanase as a probe |
-
2003
- 2003-09-22 MX MXPA05001945A patent/MXPA05001945A/es active IP Right Grant
- 2003-09-22 RU RU2005112240/13A patent/RU2359037C2/ru not_active IP Right Cessation
- 2003-09-22 PL PL376709A patent/PL376709A1/pl unknown
- 2003-09-22 CA CA002499537A patent/CA2499537A1/fr not_active Abandoned
- 2003-09-22 BR BRPI0314654A patent/BRPI0314654B8/pt not_active IP Right Cessation
- 2003-09-22 WO PCT/FR2003/002782 patent/WO2004027087A2/fr active Application Filing
- 2003-09-22 JP JP2004537235A patent/JP4373917B2/ja not_active Expired - Fee Related
- 2003-09-22 AU AU2003279430A patent/AU2003279430B2/en not_active Ceased
- 2003-09-22 EP EP03772376A patent/EP1558755B1/fr not_active Expired - Lifetime
- 2003-09-22 KR KR1020057004922A patent/KR20050057550A/ko not_active Application Discontinuation
- 2003-09-22 RS YUP-2005/0230A patent/RS20050230A/sr unknown
- 2003-09-22 CN CN2009100034115A patent/CN101747385B/zh not_active Expired - Lifetime
-
2005
- 2005-03-03 MA MA28129A patent/MA27393A1/fr unknown
- 2005-03-18 CO CO05025348A patent/CO5721022A2/es not_active Application Discontinuation
- 2005-03-21 IL IL167569A patent/IL167569A/en not_active IP Right Cessation
- 2005-03-22 HR HR20050273A patent/HRP20050273A2/xx not_active Application Discontinuation
- 2005-04-08 NO NO20051744A patent/NO20051744L/no not_active Application Discontinuation
-
2006
- 2006-02-10 HK HK06101774A patent/HK1081601A1/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| JP2006500019A (ja) | 2006-01-05 |
| HRP20050273A2 (en) | 2006-05-31 |
| CA2499537A1 (fr) | 2004-04-01 |
| KR20050057550A (ko) | 2005-06-16 |
| MA27393A1 (fr) | 2005-06-01 |
| BR0314654A (pt) | 2005-08-02 |
| WO2004027087A3 (fr) | 2005-06-09 |
| NO20051744L (no) | 2005-04-08 |
| BRPI0314654B8 (pt) | 2021-05-25 |
| EP1558755B1 (fr) | 2012-10-24 |
| PL376709A1 (pl) | 2006-01-09 |
| BRPI0314654B1 (pt) | 2016-01-05 |
| RS20050230A (en) | 2007-06-04 |
| RU2005112240A (ru) | 2005-11-20 |
| JP4373917B2 (ja) | 2009-11-25 |
| HK1081601A1 (en) | 2006-05-19 |
| AU2003279430B2 (en) | 2008-09-11 |
| EP1558755A2 (fr) | 2005-08-03 |
| AU2003279430A1 (en) | 2004-04-08 |
| RU2359037C2 (ru) | 2009-06-20 |
| IL167569A (en) | 2010-12-30 |
| CN101747385A (zh) | 2010-06-23 |
| CN101747385B (zh) | 2013-03-06 |
| WO2004027087A2 (fr) | 2004-04-01 |
| MXPA05001945A (es) | 2005-04-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FC | Application refused |