CO5270016A1 - Proceso para modificar plantas - Google Patents

Proceso para modificar plantas

Info

Publication number
CO5270016A1
CO5270016A1 CO00081624A CO00081624A CO5270016A1 CO 5270016 A1 CO5270016 A1 CO 5270016A1 CO 00081624 A CO00081624 A CO 00081624A CO 00081624 A CO00081624 A CO 00081624A CO 5270016 A1 CO5270016 A1 CO 5270016A1
Authority
CO
Colombia
Prior art keywords
plant
seeds
cell
transformed
demethylesterols
Prior art date
Application number
CO00081624A
Other languages
English (en)
Inventor
Mark Harker
Susan Amanda Hellyer
Holberg Niklas
Richard Safford
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Publication of CO5270016A1 publication Critical patent/CO5270016A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

El uso de un gen que expresa una HMG reductasa inhibida sin retroalimentación para incrementar el nivel de 4-desmetilesteroles en las semillas de las plantas.1 El método para obtener semillas mediante(a) transformar una planta por:1. transformar una célula de la planta con un constructo de DNA recombinante que comprenda un segmento de DNA que codifique un polipéptido con una actividad HMGR inhibida sin retroalimentación y un promotor para dirigir la expresión de dicho polipéptido en dicha célula de la planta para formar una célula transformada de la planta.2. regenerar la célula transformada de la planta en la planta transgénica.3. Seleccionar plantas transgénicas que tengan niveles aumentados de 4-desmetilesteroles en las semillas comparados con las cepas silvestres de la misma planta.(b) Cultivar las plantas transformadas por una o más generaciones.(c) Recolectar semillas de la planta que creció según (b).
CO00081624A 1999-10-27 2000-10-26 Proceso para modificar plantas CO5270016A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP99308515 1999-10-27

Publications (1)

Publication Number Publication Date
CO5270016A1 true CO5270016A1 (es) 2003-04-30

Family

ID=8241702

Family Applications (1)

Application Number Title Priority Date Filing Date
CO00081624A CO5270016A1 (es) 1999-10-27 2000-10-26 Proceso para modificar plantas

Country Status (7)

Country Link
EP (1) EP1224293A1 (es)
AR (1) AR026229A1 (es)
AU (1) AU1019501A (es)
BR (1) BR0014934A (es)
CA (1) CA2389084A1 (es)
CO (1) CO5270016A1 (es)
WO (1) WO2001031027A1 (es)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6822142B2 (en) 2001-01-05 2004-11-23 Monsanto Company Transgenic plants containing altered levels of steroid compounds
US6998471B2 (en) 2001-07-25 2006-02-14 E.I. Du Pont De Nemours And Company Polynucleotides encoding an acetyl-CoA acetyltransferase from Hevea brasiliensis, related products, and methods
EP1521838A1 (en) * 2002-07-16 2005-04-13 Unilever N.V. Method for modifying plants
WO2007004104A2 (en) * 2005-07-01 2007-01-11 University Of Kentucky Research Foundation Transformed plants accumulating mono- and/or sesquiterpenes
WO2012085808A1 (en) * 2010-12-20 2012-06-28 Basf Plant Science Company Gmbh Increased avenasterol production
ES2395567B1 (es) * 2011-06-22 2013-12-26 Consejo Superior De Investigaciones Científicas (Csic) Aceite de girasol con elevado contenido en fitoesteroles
JP2019041712A (ja) * 2017-09-05 2019-03-22 株式会社デンソー 組み換え体及びスクアレンの製造方法
WO2019113387A1 (en) 2017-12-07 2019-06-13 Zymergen Inc. Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5306862A (en) * 1990-10-12 1994-04-26 Amoco Corporation Method and composition for increasing sterol accumulation in higher plants
FR2687284B1 (fr) * 1992-02-14 1995-06-23 Verneuil Rech Plante portant des genes codant pour des enzymes de la voie de biosynthese des phytosterols, et procede d'obtention.
JPH09121863A (ja) * 1995-11-02 1997-05-13 Sumitomo Chem Co Ltd 植物の改変方法及び改変植物
AU2089197A (en) * 1996-03-13 1997-10-01 National Research Council Of Canada Process of raising squalene levels in plants and dna sequences used therefor
US5952545A (en) * 1996-03-27 1999-09-14 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Nucleic acid molecules encoding cytochrome P450-type proteins involved in the brassinosteroid synthesis in plants
EP0954568A1 (en) * 1996-06-21 1999-11-10 The General Hospital Corporation Plant sterol reductases and uses thereof
WO1998045457A1 (en) * 1996-12-26 1998-10-15 Monsanto Company Transgenic plants with modified sterol biosynthetic pathways
WO2000061771A2 (en) * 1999-04-12 2000-10-19 Monsanto Technology Llc Transgenic plants containing altered levels of sterol compounds and tocopherols

Also Published As

Publication number Publication date
AR026229A1 (es) 2003-01-29
EP1224293A1 (en) 2002-07-24
BR0014934A (pt) 2002-06-18
WO2001031027A1 (en) 2001-05-03
CA2389084A1 (en) 2001-05-03
AU1019501A (en) 2001-05-08

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Legal Events

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