CO4230106A1 - Proceso para la preparacion de una nueva aminopeptidasa y su proteina - Google Patents
Proceso para la preparacion de una nueva aminopeptidasa y su proteinaInfo
- Publication number
- CO4230106A1 CO4230106A1 CO94026075A CO94026075A CO4230106A1 CO 4230106 A1 CO4230106 A1 CO 4230106A1 CO 94026075 A CO94026075 A CO 94026075A CO 94026075 A CO94026075 A CO 94026075A CO 4230106 A1 CO4230106 A1 CO 4230106A1
- Authority
- CO
- Colombia
- Prior art keywords
- protein
- preparation
- aminopeptidase
- new
- new aminopeptidase
- Prior art date
Links
- 102000004400 Aminopeptidases Human genes 0.000 title abstract 3
- 108090000915 Aminopeptidases Proteins 0.000 title abstract 3
- 102000004169 proteins and genes Human genes 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 abstract 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 abstract 1
- 241000187177 Streptomyces thermovulgaris Species 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/485—Exopeptidases (3.4.11-3.4.19)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/61—Growth hormone [GH], i.e. somatotropin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/11—Aminopeptidases (3.4.11)
- C12Y304/11002—Membrane alanyl aminopeptidase (3.4.11.2), i.e. aminopeptidase N
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Biomedical Technology (AREA)
- Analytical Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
UNA NUEVA AMINOPEPTIDASA SEPARADA DE UN CULTIVO MEDIO DE STREPTOMUCES THERMONITRIFICANS. 2. LA AMINOPEPTIDASA DE LA REIVINDICACION 1 QUE COMPRENDE UNA SECUENCIA DE AMINO ACIDO DE Lys-Phe- Ser-Lys-Lys-Phe--Asn-Glu EN LA N-TERMINAL Y LA SE- CUENCIA DEL AMINO ACIDO DE Glu-Pro-Gly-Thr-Gly- Ala-Leu-Glu-Pro y Asn-Pro-Asp-lle-Val-Thr y OTRAS REGIONES. 3. LA AMINOPEPTIDASA DE LA REIVINDICACION 1 TENI- ENDO UN PESO MOLECULAR DE UN RANGO DE 41 A 45 kd EN UN ESTADO DE FORMA REDUCIDA Y UN PESO MOLECULAR EN EL RANGO DE 36 A 40 kd EN UN ESTADO DE FORMA NATURAL. 4. LA AMINOPEPETIDASA DE LA REIVINDICACION 1 TENI- ENDO CONDICIONES DE ACTIVIDAD DE UN Ph DENTRO DEL RANGO DE 7.5 A 9.0 Y TEMPERATURA ENTRE UN RANGO DE 30 A 50 GRADOS CENTIGRADOS. 5. UN PROCESO PARA PREPARAR UN AMONOPEPTIDASA DE- FINIFO EN CADA UNA DE LAS PETICIONES 1 A 4 COM- PRENDIENDO LOS PASOS DE CULTIVACION STREPTOMYCES THERMONITRIFICANS EN UN MEDIO QUE CONTIENE GALAC- TOSA COMO UNA FUENTE DE CARBON Y RECOBRANDO LA AMINOPEPTIDASA DE UN CULTIVO MEDIO. 6. EL PROCESO DE LA REIVINDICACION 5 EN DONDE LA CONCENTRACION DE LA GALACTOSA EN LOS MEDIOS RANGOS DE 1 A 5% POR PESO SOBRE LA BASE DEL PESO TOTAL DEL MEDIO. 7. EL PROCESO DE LA REIVINDICACION 5 0 6 QUE ADE- MAS COMPRENDE LOS PASOS DE : a) CENTRIFUGACION DEL CULTIVO DE LA STREPTOMYCES THERMONITRIFICANS Y SEPARANDO LA SUPERNATA DE ESTE b) AGREGANDO SULFATO DE AMONIO A LA SUPERNATA; c) LLEVANDO A CABO UNA PARTICION TRIFASICA ANADI- ENDO BUTANOL TERCIARIO A LA SUPERNATA Y SEPARANDO UNA SOLUCION CLARA COMO LA CAPA INFERIOR; d) AGREGANDO SULFATO DE AMONIO A LA SOLUCION CLARA Y REPITIENDO EL PASO c) PERO SEPARANDO UNA CAPA INTERMEDIA ENTRE DICHO BUTANOL TERCIARIO Y DICHA CAPA AQUOSA; e) SOMETIENDO LA CAPA INTERMEDIA OBTENIDA EN DICHO PASO d) CAMBIAR EL CATION DE CROMATOFRAGIA PARA OBTENER FRACCIONES QUE CONTIENE LA AMINOPEPTIDASA; Y f) CONDUCIENDO FPLC CON RESPECTO A LAS FRACCIONES PARA OBTENER LA AMINOPEPTIDASA EN UN ALTO GRADO DE PUREZA 8) EL PROCESO DE LA REIVINDICACION 7 EN DONDE DI- CHO SULFATO DE AMONIO ES ANADIDO EN DICHOS PASOS b) y d) PARA CONCENTRACIONES DE RANGOS DE 35 A 50 (wt/vol) Y DE 80 A 90% (wt/vol) RESPECTIVAMENTE. 9. EL PROCESO DE LA REIVINDICACION 7 EN DONDE DI- CHO CAMBIO CROMATOGRAFIA DE CATION ES CONDUCIDO MEDIANTE EL EMPLEO DE UNA COLUMAN S-SEFAROSA. EL PROCESO DE LA REIVINDICACION 7 DONDE DICHA COLUMNA FPLC DE CROMATOGRAFIA ES CONDUCIDA EMPLE- ANDO UNA COLUMNA FPLC MONO-S. 11. UN PROCESO PARA PREPARAR UNA PROTEINA RECOMBI- NANTE N-METIONO CON LA AMINOPEPTIDASA CITADA EN LA REIVINDICACION 1 EN UNBUFFER DE REACCION.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1019930011107A KR970010135B1 (ko) | 1993-06-17 | 1993-06-17 | 신규 아미노펩티다제 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CO4230106A1 true CO4230106A1 (es) | 1995-10-19 |
Family
ID=19357590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CO94026075A CO4230106A1 (es) | 1993-06-17 | 1994-06-16 | Proceso para la preparacion de una nueva aminopeptidasa y su proteina |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5569598A (es) |
| EP (1) | EP0629695B1 (es) |
| JP (1) | JP2890168B2 (es) |
| KR (1) | KR970010135B1 (es) |
| CN (1) | CN1098742A (es) |
| AT (1) | ATE206159T1 (es) |
| BR (1) | BR9402461A (es) |
| CO (1) | CO4230106A1 (es) |
| DE (1) | DE69428395T2 (es) |
| EG (1) | EG21684A (es) |
| ES (1) | ES2160605T3 (es) |
| MY (1) | MY110997A (es) |
| PE (1) | PE50794A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100327971B1 (ko) * | 1999-04-07 | 2002-03-14 | 허영섭 | 아미노 말단 메티오닌이 제거된 재조합 인터페론-α의 제조방법 |
| KR100365838B1 (ko) * | 2000-03-24 | 2002-12-26 | 한국생명공학연구원 | 브레비바실러스 보스테렌시스 ｂｃｓ-1 에서 유래한 신규내열성 ｄ-입체특이적 디펩티다아제 및 이를 이용한ｄ-아미노산 함유 펩타이드의 합성 방법 |
| EP1306430A4 (en) * | 2000-07-31 | 2004-09-08 | Takeda Chemical Industries Ltd | METHOD FOR PRODUCING A RECOMBINANT PROTEIN |
| EP2097520A4 (en) * | 2006-11-30 | 2012-07-04 | Biocon Ltd | METHOD FOR MANUFACTURING N-TERMINAL METHIONINE EXEMPT POLYPEPTIDES IN MICROBIAL HOST CELLS |
| EP2206777B1 (en) * | 2007-10-29 | 2014-01-15 | JCR Pharmaceuticals CO., LTD. | Method for producing aminopeptidase |
| CN111449318B (zh) * | 2020-03-18 | 2021-12-28 | 同曦集团有限公司 | 一种抗菌抗病毒口罩及其制备方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1536822A (en) * | 1976-05-31 | 1978-12-20 | Sumitomo Chemical Co | Immobilized non-specific proteases |
| DK55685A (da) * | 1985-02-07 | 1986-08-08 | Nordisk Gentofte | Enzym eller enzymkompleks med proteolytisk aktivitet |
| EP0489711B1 (en) * | 1984-08-16 | 2003-10-01 | Savient Pharmaceuticals, Inc. | Method for producing human growth hormone |
| WO1986007380A1 (en) * | 1985-06-04 | 1986-12-18 | Takeda Chemical Industries, Ltd. | Process for preparing polypeptide |
| GB8703015D0 (en) * | 1987-02-10 | 1987-03-18 | Biogen Nv | Aminopeptidase |
| KR910000458B1 (ko) * | 1988-12-31 | 1991-01-25 | 주식회사 럭키 | 인간성장호르몬 발현 벡터 pYLBC-GAP-HGH |
| IL91235A (en) * | 1989-08-07 | 1994-10-07 | Univ Ramot | Aminopeptidase and a process for its purification |
| KR920000099B1 (ko) * | 1990-03-15 | 1992-01-09 | 주식회사 럭키 | 효모에서 발현된 인간성장호르몬의 정제방법 |
-
1993
- 1993-06-17 KR KR1019930011107A patent/KR970010135B1/ko not_active IP Right Cessation
-
1994
- 1994-06-15 PE PE1994244636A patent/PE50794A1/es not_active Application Discontinuation
- 1994-06-15 MY MYPI94001525A patent/MY110997A/en unknown
- 1994-06-16 DE DE69428395T patent/DE69428395T2/de not_active Expired - Fee Related
- 1994-06-16 EP EP94109309A patent/EP0629695B1/en not_active Expired - Lifetime
- 1994-06-16 AT AT94109309T patent/ATE206159T1/de not_active IP Right Cessation
- 1994-06-16 ES ES94109309T patent/ES2160605T3/es not_active Expired - Lifetime
- 1994-06-16 CO CO94026075A patent/CO4230106A1/es unknown
- 1994-06-17 JP JP6135268A patent/JP2890168B2/ja not_active Expired - Fee Related
- 1994-06-17 US US08/261,525 patent/US5569598A/en not_active Expired - Fee Related
- 1994-06-17 BR BR9402461A patent/BR9402461A/pt not_active Application Discontinuation
- 1994-06-17 EG EG36094A patent/EG21684A/xx active
- 1994-06-17 CN CN94107221A patent/CN1098742A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| KR970010135B1 (ko) | 1997-06-21 |
| ES2160605T3 (es) | 2001-11-16 |
| ATE206159T1 (de) | 2001-10-15 |
| US5569598A (en) | 1996-10-29 |
| DE69428395D1 (de) | 2001-10-31 |
| EP0629695B1 (en) | 2001-09-26 |
| CN1098742A (zh) | 1995-02-15 |
| EP0629695A1 (en) | 1994-12-21 |
| KR950000889A (ko) | 1995-01-03 |
| MY110997A (en) | 1999-07-31 |
| BR9402461A (pt) | 1995-01-24 |
| PE50794A1 (es) | 1995-01-14 |
| EG21684A (en) | 2002-02-27 |
| JP2890168B2 (ja) | 1999-05-10 |
| DE69428395T2 (de) | 2002-06-06 |
| JPH07147979A (ja) | 1995-06-13 |
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