EP2097520A4 - A method for producing n-terminal methioine free polypeptides in microbial host cells - Google Patents

A method for producing n-terminal methioine free polypeptides in microbial host cells

Info

Publication number
EP2097520A4
EP2097520A4 EP07706183A EP07706183A EP2097520A4 EP 2097520 A4 EP2097520 A4 EP 2097520A4 EP 07706183 A EP07706183 A EP 07706183A EP 07706183 A EP07706183 A EP 07706183A EP 2097520 A4 EP2097520 A4 EP 2097520A4
Authority
EP
European Patent Office
Prior art keywords
methioine
producing
terminal
host cells
microbial host
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP07706183A
Other languages
German (de)
French (fr)
Other versions
EP2097520A1 (en
Inventor
Kedarnath Sastry
Akundi Venkata Sriram
Anuj Goel
Sandeep Vishwanath Kamath
Hardik Valera
Mayank Kumar Garg
Suma Sreenivas
Malur Dattatreya Swetha
Amrita Basu
Reena Nichinmetla Raghunandan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biocon Ltd
Original Assignee
Biocon Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocon Ltd filed Critical Biocon Ltd
Publication of EP2097520A1 publication Critical patent/EP2097520A1/en
Publication of EP2097520A4 publication Critical patent/EP2097520A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • A61K38/22—Hormones
    • A61K38/27—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP07706183A 2006-11-30 2007-01-12 A method for producing n-terminal methioine free polypeptides in microbial host cells Withdrawn EP2097520A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IN2217CH2006 2006-11-30
PCT/IN2007/000012 WO2008065673A1 (en) 2006-11-30 2007-01-12 A method for producing n-terminal methioine free polypeptides in microbial host cells

Publications (2)

Publication Number Publication Date
EP2097520A1 EP2097520A1 (en) 2009-09-09
EP2097520A4 true EP2097520A4 (en) 2012-07-04

Family

ID=39467497

Family Applications (1)

Application Number Title Priority Date Filing Date
EP07706183A Withdrawn EP2097520A4 (en) 2006-11-30 2007-01-12 A method for producing n-terminal methioine free polypeptides in microbial host cells

Country Status (3)

Country Link
EP (1) EP2097520A4 (en)
MX (1) MX2009005764A (en)
WO (1) WO2008065673A1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102533712A (en) * 2009-05-12 2012-07-04 中国科学院上海生命科学研究院 Solid-phase SUMO (Small Ubiquitin-like Modifier) conjugation system and solid-phase SUMO de-conjugation system

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057174A2 (en) * 2002-01-07 2003-07-17 Lifesensors, Inc. Methods and compositions for protein expression and purification
WO2006073976A2 (en) * 2004-12-30 2006-07-13 Lifesensors, Inc. Compositions, methods, and kits for enhancing protein expression, solubility and isolation

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763215A (en) * 1984-08-16 1998-06-09 Bio-Technology General Corporation Method of removing N-terminal amino acid residues from eucaryotic polypeptide analogs and polypeptides produced thereby
KR970010135B1 (en) * 1993-06-17 1997-06-21 주식회사 엘지화학 Novel aminopeptidase isolated from streptococcus thermonitrificans
KR100369839B1 (en) * 1997-02-28 2003-06-12 주식회사 엘지생명과학 Aminopeptidase isolated from bacillus licheniformis, preparation process thereof, and process for producing natural protein using the same enzyme
US7109015B2 (en) * 2004-03-29 2006-09-19 Academia Sinica Removal of N-terminal methionine from proteins by engineered methionine aminopeptidase

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003057174A2 (en) * 2002-01-07 2003-07-17 Lifesensors, Inc. Methods and compositions for protein expression and purification
WO2006073976A2 (en) * 2004-12-30 2006-07-13 Lifesensors, Inc. Compositions, methods, and kits for enhancing protein expression, solubility and isolation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BEN-BASSAT A: "Methods for removing N-terminal methionine from recombinant proteins", BIOPROCESS TECHNOLOGY, DEKKER, US, vol. 12, 1 January 1991 (1991-01-01), pages 147 - 159, XP009159378, ISSN: 0888-7470 *
L. V. MUNDADA: "Structure-Function Analysis of the Streptokinase Amino Terminus (Residues 1-59)", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 278, no. 27, 1 January 2003 (2003-01-01), pages 24421 - 24427, XP055027055, ISSN: 0021-9258, DOI: 10.1074/jbc.M301825200 *
See also references of WO2008065673A1 *

Also Published As

Publication number Publication date
MX2009005764A (en) 2009-08-24
EP2097520A1 (en) 2009-09-09
WO2008065673A1 (en) 2008-06-05

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Effective date: 20120605

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Ipc: C12N 15/18 20060101ALI20120530BHEP

Ipc: C12N 9/70 20060101AFI20120530BHEP

Ipc: A61K 38/27 20060101ALI20120530BHEP

Ipc: C07K 14/61 20060101ALI20120530BHEP

Ipc: C12N 15/57 20060101ALI20120530BHEP

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