PE50794A1 - Aminopeptidasa, proceso para la preparacion de la aminopeptidasa y de la proteina resultante - Google Patents

Aminopeptidasa, proceso para la preparacion de la aminopeptidasa y de la proteina resultante

Info

Publication number
PE50794A1
PE50794A1 PE1994244636A PE24463694A PE50794A1 PE 50794 A1 PE50794 A1 PE 50794A1 PE 1994244636 A PE1994244636 A PE 1994244636A PE 24463694 A PE24463694 A PE 24463694A PE 50794 A1 PE50794 A1 PE 50794A1
Authority
PE
Peru
Prior art keywords
aminopeptidase
preparation
resulting protein
wild
culture
Prior art date
Application number
PE1994244636A
Other languages
English (en)
Inventor
Young Mee Lee
Teug Yeon Won
Soon Jae Park
Soon Chang Kwon
Seung Joo Lee
Jung Ho Kim
Bum Joon Kim
Original Assignee
Lucky Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lucky Ltd filed Critical Lucky Ltd
Publication of PE50794A1 publication Critical patent/PE50794A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/11—Aminopeptidases (3.4.11)
    • C12Y304/11002—Membrane alanyl aminopeptidase (3.4.11.2), i.e. aminopeptidase N

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SE REFIERE A UNA AMINOPEPTIDASA SEPARADA DE UN MEDIO DE CULTIVO DE STREPTOMYCES THERMONITRIFICANS, QUE CONTIENE GALACTOSA COMO FUENTE DE CARBONO; LA CUAL INCLUYE UNA SECUENCIA AMINOACIDA DE LYS-PHE-SER-LYS-LYS-PHE-ASN-GLU EN SU TERMINAL N Y SECUENCIAS AMINOACIDAS DE GLU-PRO-GLY-THR-GLY-ALA-LEU-GLU-PRO Y ASN-PRO-ASP-ILE-VAL-THR EN OTRAS DE SUS REGIONES; TENIENDO UN PESO MOLECULAR DE 41 KD A 45 KD EN ESTADO REDUCIDO Y 36 KD A 40 KD EN ESTADO NATURAL, LA CUAL PUEDE SER HECHA REACCIONAR CON UNA PROTEINA RECOMBINANTE N-METIONILO TAL COMO UNA HORMONA RECOMBINANTE DE CRECIMIENTO HUMANO DERIVADA DE UN MICROORGANISMO COMO E. COLI Y/O LEVADURA, DONDE LA AMINOPEPTIDASA CAUSA LA ELIMINACION DE LA METIONINA DEL TERMINAL N LO QUE PODRIA EVITAR UNA NO DESEADA REACCION INMUNE AL ADMINISTRARSE DICHA PROTEINA RECOMBINANTE A UN HUMANO
PE1994244636A 1993-06-17 1994-06-15 Aminopeptidasa, proceso para la preparacion de la aminopeptidasa y de la proteina resultante PE50794A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
KR1019930011107A KR970010135B1 (ko) 1993-06-17 1993-06-17 신규 아미노펩티다제

Publications (1)

Publication Number Publication Date
PE50794A1 true PE50794A1 (es) 1995-01-14

Family

ID=19357590

Family Applications (1)

Application Number Title Priority Date Filing Date
PE1994244636A PE50794A1 (es) 1993-06-17 1994-06-15 Aminopeptidasa, proceso para la preparacion de la aminopeptidasa y de la proteina resultante

Country Status (13)

Country Link
US (1) US5569598A (es)
EP (1) EP0629695B1 (es)
JP (1) JP2890168B2 (es)
KR (1) KR970010135B1 (es)
CN (1) CN1098742A (es)
AT (1) ATE206159T1 (es)
BR (1) BR9402461A (es)
CO (1) CO4230106A1 (es)
DE (1) DE69428395T2 (es)
EG (1) EG21684A (es)
ES (1) ES2160605T3 (es)
MY (1) MY110997A (es)
PE (1) PE50794A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100327971B1 (ko) * 1999-04-07 2002-03-14 허영섭 아미노 말단 메티오닌이 제거된 재조합 인터페론-α의 제조방법
KR100365838B1 (ko) * 2000-03-24 2002-12-26 한국생명공학연구원 브레비바실러스 보스테렌시스 ｂｃｓ-1 에서 유래한 신규내열성 ｄ-입체특이적 디펩티다아제 및 이를 이용한ｄ-아미노산 함유 펩타이드의 합성 방법
WO2002010370A1 (fr) * 2000-07-31 2002-02-07 Takeda Chemical Industries, Ltd. Procede de production d'une proteine recombinee
EP2097520A4 (en) * 2006-11-30 2012-07-04 Biocon Ltd METHOD FOR MANUFACTURING N-TERMINAL METHIONINE EXEMPT POLYPEPTIDES IN MICROBIAL HOST CELLS
US8685691B2 (en) * 2007-10-29 2014-04-01 Jcr Pharmaceuticals Co., Ltd. Method for producing aminopeptidase
CN111449318B (zh) * 2020-03-18 2021-12-28 同曦集团有限公司 一种抗菌抗病毒口罩及其制备方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1536822A (en) * 1976-05-31 1978-12-20 Sumitomo Chemical Co Immobilized non-specific proteases
DK55685A (da) * 1985-02-07 1986-08-08 Nordisk Gentofte Enzym eller enzymkompleks med proteolytisk aktivitet
ATE251217T1 (de) * 1984-08-16 2003-10-15 Savient Pharmaceuticals Inc Methode zur herstellung von menschlichen wachstumshormonen
WO1986007380A1 (en) * 1985-06-04 1986-12-18 Takeda Chemical Industries, Ltd. Process for preparing polypeptide
GB8703015D0 (en) * 1987-02-10 1987-03-18 Biogen Nv Aminopeptidase
KR910000458B1 (ko) * 1988-12-31 1991-01-25 주식회사 럭키 인간성장호르몬 발현 벡터 pYLBC-GAP-HGH
IL91235A (en) * 1989-08-07 1994-10-07 Univ Ramot Aminopeptidase and a process for its purification
KR920000099B1 (ko) * 1990-03-15 1992-01-09 주식회사 럭키 효모에서 발현된 인간성장호르몬의 정제방법

Also Published As

Publication number Publication date
DE69428395T2 (de) 2002-06-06
KR970010135B1 (ko) 1997-06-21
ES2160605T3 (es) 2001-11-16
JPH07147979A (ja) 1995-06-13
ATE206159T1 (de) 2001-10-15
EG21684A (en) 2002-02-27
JP2890168B2 (ja) 1999-05-10
EP0629695A1 (en) 1994-12-21
BR9402461A (pt) 1995-01-24
DE69428395D1 (de) 2001-10-31
MY110997A (en) 1999-07-31
CN1098742A (zh) 1995-02-15
KR950000889A (ko) 1995-01-03
US5569598A (en) 1996-10-29
EP0629695B1 (en) 2001-09-26
CO4230106A1 (es) 1995-10-19

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Legal Events

Date Code Title Description
FG Grant, registration
FD Application declared void or lapsed