CO2019010530A2 - Métodos para mejorar rasgos en plantas - Google Patents

Métodos para mejorar rasgos en plantas

Info

Publication number
CO2019010530A2
CO2019010530A2 CO2019010530A CO2019010530A CO2019010530A2 CO 2019010530 A2 CO2019010530 A2 CO 2019010530A2 CO 2019010530 A CO2019010530 A CO 2019010530A CO 2019010530 A CO2019010530 A CO 2019010530A CO 2019010530 A2 CO2019010530 A2 CO 2019010530A2
Authority
CO
Colombia
Prior art keywords
plants
methods
trait
steps
genetic material
Prior art date
Application number
CO2019010530A
Other languages
English (en)
Inventor
Dror Shalitin
Noam Grimberg
Arava Shatil Cohen
Original Assignee
Plantarcbio Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plantarcbio Ltd filed Critical Plantarcbio Ltd
Publication of CO2019010530A2 publication Critical patent/CO2019010530A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención revela un método para la detección y la identificación de un rasgo deseable de mejora de las plantas, dicho método comprende los pasos de: (a) la obtención de material genético de una muestra de una fuente predefinida y (b) la construcción de una biblioteca de expresión a partir de dicho material genético. El método mencionado con anterioridad además comprende los pasos de: (c) la producción de plantas transformadas con dicha biblioteca de expresión con una eficacia de transformación de por lo menos 0,05% a 30%, lo que representa por lo menos de 102 a 1010 transgenes; (d) la detección de las plantas transformadas que expresan dicho rasgo deseable; y (e) la identificación de dicho transgén de dichas plantas transformadas que expresan dicho rasgo deseable.
CO2019010530A 2017-03-28 2019-09-26 Métodos para mejorar rasgos en plantas CO2019010530A2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762477517P 2017-03-28 2017-03-28
US201862644600P 2018-03-19 2018-03-19
PCT/IL2018/050349 WO2018178975A1 (en) 2017-03-28 2018-03-27 Methods for improving traits in plants

Publications (1)

Publication Number Publication Date
CO2019010530A2 true CO2019010530A2 (es) 2019-10-31

Family

ID=63674330

Family Applications (1)

Application Number Title Priority Date Filing Date
CO2019010530A CO2019010530A2 (es) 2017-03-28 2019-09-26 Métodos para mejorar rasgos en plantas

Country Status (13)

Country Link
US (5) US11111491B2 (es)
EP (1) EP3599829B1 (es)
CN (1) CN110691509A (es)
AU (2) AU2018242633B2 (es)
BR (1) BR112019020167A2 (es)
CA (1) CA3057004A1 (es)
CL (1) CL2019002757A1 (es)
CO (1) CO2019010530A2 (es)
ES (1) ES2964023T3 (es)
MX (2) MX2019011579A (es)
PH (1) PH12019502244A1 (es)
WO (1) WO2018178975A1 (es)
ZA (1) ZA201906391B (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113584076B (zh) * 2021-08-31 2023-09-15 西南大学 沉默桑实杯盘菌G蛋白β亚基编码基因CsGβ2的沉默载体及其应用和方法
CN115948426B (zh) * 2022-12-19 2024-02-20 海南大学 一种炭疽菌CsATLP基因及应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6972183B1 (en) 1997-06-16 2005-12-06 Diversa Corporation Capillary array-based enzyme screening
US6368798B1 (en) 1995-12-07 2002-04-09 Diversa Corporation Screening for novel bioactivities
CR5828A (es) * 1997-07-30 1999-10-25 Wisconsin Alumni Res Found Plasma de germen y marcadores moleculares para resistenciaen enfermedades de papas
AU770540B2 (en) 1998-04-30 2004-02-26 Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. Novel method for the identification of clones conferring a desired biological property from an expression library
US7723110B2 (en) * 2005-06-17 2010-05-25 Arborgen, Llc Plant cell signaling genes
US20110252501A1 (en) * 2006-08-17 2011-10-13 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
CL2007002532A1 (es) 2006-08-31 2008-01-11 Monsanto Technology Llc Soc Organizada Bajo Las Leyes Del Estado De Delaware Procedimiento para identificar genes que confieren rasgos mejorados de una población de plantas.
US7867459B2 (en) 2008-07-15 2011-01-11 Sidney Leroy Born Compact, tiered sulfur recovery unit
US8937215B2 (en) 2009-08-04 2015-01-20 Evogene Ltd. Polynucleotides and polypeptides for increasing desirable plant qualities
WO2012143865A1 (en) * 2011-04-22 2012-10-26 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and method for making the same
BR112015015415B1 (pt) * 2012-12-25 2022-08-16 Evogene Ltd. Métodos para aumentar a eficiência no uso do nitrogênio, taxa de crescimento, biomassa, rendimento da semente, capacidade fotossintética e/ou tolerância ao estresse abiótico de uma planta, para produzir de uma cultura, para cultivar uma colheita, e, para selecionar uma planta
CN103382476B (zh) * 2013-06-06 2015-04-08 江苏省农业科学院 赋予植物耐寒性的一个棉花P型ATP酶基因Gbpatp的应用
WO2016095124A1 (en) 2014-12-17 2016-06-23 Kunming Institute Of Botany, The Chinese Academy Of Sciences Compositions and methods for increasing drought tolerance in plants

Also Published As

Publication number Publication date
US20210388340A1 (en) 2021-12-16
US20240141329A1 (en) 2024-05-02
EP3599829A4 (en) 2020-07-29
CN110691509A (zh) 2020-01-14
EP3599829B1 (en) 2023-10-04
US20240209351A1 (en) 2024-06-27
US11905512B2 (en) 2024-02-20
MX2023004224A (es) 2023-04-21
WO2018178975A1 (en) 2018-10-04
US20200115704A1 (en) 2020-04-16
ES2964023T3 (es) 2024-04-03
CL2019002757A1 (es) 2020-03-06
AU2018242633B2 (en) 2024-02-08
US12054709B2 (en) 2024-08-06
EP3599829A1 (en) 2020-02-05
AU2024200329A1 (en) 2024-02-15
EP3599829C0 (en) 2023-10-04
ZA201906391B (en) 2020-09-30
US11111491B2 (en) 2021-09-07
WO2018178975A9 (en) 2018-11-29
PH12019502244A1 (en) 2020-06-29
AU2018242633A1 (en) 2019-10-17
CA3057004A1 (en) 2018-10-04
MX2019011579A (es) 2020-01-20
BR112019020167A2 (pt) 2020-06-02
US20230313179A1 (en) 2023-10-05

Similar Documents

Publication Publication Date Title
BR112019002455A2 (pt) método para edição de base em plantas
CO2017010354A2 (es) Proceso para la cría de insectos
UY35613A (es) ?molecula de adn recombinante del evento de soja mon87751, métodos de detección, plantas y semillas y método para protegerlas?.
CL2019001431A1 (es) Aparato y procedimiento para plantación de árboles.
AR088858A1 (es) Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas
PE20171381A1 (es) Genes de determinacion del sexo y su uso en el cultivo
CO2018011670A2 (es) Planta de tabaco y método para producir la misma
CL2017001665A1 (es) Inductor haploide
GT201400070A (es) Sistema para plantas programable
AR085892A1 (es) Metodo para desarrollar soja con un contenido elevado de acido oleico usando tecnicas de desarrollo convencionales
CO2019007828A2 (es) Evento de élite ee-gm5 y métodos y kits para identificar dicho evento en muestras biológicas
AR070693A1 (es) Plantas que tienen rasgos aumentados relacionados con el rendimiento y un metodo para obtenerlas a partir de la expresion del polipeptido de nitrato 2 (nrt2)
CO2019010530A2 (es) Métodos para mejorar rasgos en plantas
CL2017001298A1 (es) Nuevas proteínas inhibidoras de insectos
BR112016028496A2 (pt) análise quantitativa de proteínas transgênicas
CL2017001409A1 (es) Mapeo fino y validación de qtl que subyace al contenido de fibra y a los caracteres del color de la cubierta de la semilla e identificación de marcadores de snp para selección asistida por marcador de estos caracteres derivados de la línea de canola con cubierta de semilla amarilla (ysc) yn01-429 y su linaje.
AR105760A1 (es) Métodos y constructos para la modificación específica de ácidos nucleicos en plantas
CO2019007826A2 (es) Evento de élite ee-gm4 y métodos y kits para identificar dicho evento en muestras biológicas
CO2018000499A2 (es) Métodos y composiciones para la regulación selectiva de la expresión de proteínas
BR112018000541A2 (pt) métodos para produção de plantas de algodão com tolerância à seca melhorada e composições dos mesmos.
BR112016023624A2 (pt) proteínas de antiporte prótons/ açúcar tonoplásticas e uso do mesmo para o aumento da concentração de sacarose de um órgão de plantas para armazenamento de sacarose
AR111261A1 (es) Métodos para mejorar rasgos en plantas
CL2017002400A1 (es) Un constructo de acidos nucleicos para conferir tolerancia a herbicidas a las plantas
CL2017000948A1 (es) Textile compuesto que consiste en fibras naturales y/o sintéticas y/o artificiales y partículas lignocelulósicas
AR098760A1 (es) Procedimiento mediado por rhizobiaceae libre de marcadores de selección para la producción de una planta transgénica del género triticum