CO2019010530A2 - Métodos para mejorar rasgos en plantas - Google Patents

Métodos para mejorar rasgos en plantas

Info

Publication number
CO2019010530A2
CO2019010530A2 CO2019010530A CO2019010530A CO2019010530A2 CO 2019010530 A2 CO2019010530 A2 CO 2019010530A2 CO 2019010530 A CO2019010530 A CO 2019010530A CO 2019010530 A CO2019010530 A CO 2019010530A CO 2019010530 A2 CO2019010530 A2 CO 2019010530A2
Authority
CO
Colombia
Prior art keywords
plants
methods
trait
steps
genetic material
Prior art date
Application number
CO2019010530A
Other languages
English (en)
Inventor
Dror Shalitin
Noam Grimberg
Arava Shatil Cohen
Original Assignee
Plantarcbio Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plantarcbio Ltd filed Critical Plantarcbio Ltd
Publication of CO2019010530A2 publication Critical patent/CO2019010530A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención revela un método para la detección y la identificación de un rasgo deseable de mejora de las plantas, dicho método comprende los pasos de: (a) la obtención de material genético de una muestra de una fuente predefinida y (b) la construcción de una biblioteca de expresión a partir de dicho material genético. El método mencionado con anterioridad además comprende los pasos de: (c) la producción de plantas transformadas con dicha biblioteca de expresión con una eficacia de transformación de por lo menos 0,05% a 30%, lo que representa por lo menos de 102 a 1010 transgenes; (d) la detección de las plantas transformadas que expresan dicho rasgo deseable; y (e) la identificación de dicho transgén de dichas plantas transformadas que expresan dicho rasgo deseable.
CO2019010530A 2017-03-28 2019-09-26 Métodos para mejorar rasgos en plantas CO2019010530A2 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762477517P 2017-03-28 2017-03-28
US201862644600P 2018-03-19 2018-03-19
PCT/IL2018/050349 WO2018178975A1 (en) 2017-03-28 2018-03-27 Methods for improving traits in plants

Publications (1)

Publication Number Publication Date
CO2019010530A2 true CO2019010530A2 (es) 2019-10-31

Family

ID=63674330

Family Applications (1)

Application Number Title Priority Date Filing Date
CO2019010530A CO2019010530A2 (es) 2017-03-28 2019-09-26 Métodos para mejorar rasgos en plantas

Country Status (13)

Country Link
US (5) US11111491B2 (es)
EP (1) EP3599829B1 (es)
CN (1) CN110691509A (es)
AU (2) AU2018242633B2 (es)
BR (1) BR112019020167A2 (es)
CA (1) CA3057004A1 (es)
CL (1) CL2019002757A1 (es)
CO (1) CO2019010530A2 (es)
ES (1) ES2964023T3 (es)
MX (2) MX2019011579A (es)
PH (1) PH12019502244A1 (es)
WO (1) WO2018178975A1 (es)
ZA (1) ZA201906391B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113584076B (zh) * 2021-08-31 2023-09-15 西南大学 沉默桑实杯盘菌G蛋白β亚基编码基因CsGβ2的沉默载体及其应用和方法
CN115948426B (zh) * 2022-12-19 2024-02-20 海南大学 一种炭疽菌CsATLP基因及应用
WO2024218767A1 (en) 2023-04-18 2024-10-24 Imi Tami Institute For Research & Development Ltd. Plants comprising bromoform production genes

Family Cites Families (16)

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US6030779A (en) 1995-07-18 2000-02-29 Diversa Corporation Screening for novel bioactivities
US6057103A (en) 1995-07-18 2000-05-02 Diversa Corporation Screening for novel bioactivities
US6972183B1 (en) 1997-06-16 2005-12-06 Diversa Corporation Capillary array-based enzyme screening
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6368798B1 (en) 1995-12-07 2002-04-09 Diversa Corporation Screening for novel bioactivities
CR5828A (es) * 1997-07-30 1999-10-25 Wisconsin Alumni Res Found Plasma de germen y marcadores moleculares para resistenciaen enfermedades de papas
DE69921982T2 (de) 1998-04-30 2005-12-29 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Neuartiges verfahren zur identifizierung von klonen mit einer gewünschten biologischen eigenschaft, ausgehend von einer expressionsgenbank
NZ565205A (en) * 2005-06-17 2011-02-25 Arborgen Llc Cell signalling genes from plants (Eucalyptus and Pinus species) and related methods
WO2008021543A2 (en) * 2006-08-17 2008-02-21 Monsanto Technology, Llc Transgenic plants with enhanced agronomic traits
CL2007002532A1 (es) 2006-08-31 2008-01-11 Monsanto Technology Llc Soc Organizada Bajo Las Leyes Del Estado De Delaware Procedimiento para identificar genes que confieren rasgos mejorados de una población de plantas.
US7867459B2 (en) 2008-07-15 2011-01-11 Sidney Leroy Born Compact, tiered sulfur recovery unit
CA3121387A1 (en) 2009-08-04 2011-02-10 Evogene Ltd. Isolated polynucleotides and polypeptides, and methods of using same forincreasing abiotic stress tolerance, yield, growth rate, vigor, biomass oil content, and/or nitrogen use efficiency of plants
WO2012143865A1 (en) * 2011-04-22 2012-10-26 Basf Plant Science Company Gmbh Plants having enhanced yield-related traits and method for making the same
CA3182775A1 (en) * 2012-12-25 2014-07-03 Evogene Ltd. Isolated polynucleotides and polypeptides, and methods of using same for increasing nitrogen use efficiency of plants
CN103382476B (zh) * 2013-06-06 2015-04-08 江苏省农业科学院 赋予植物耐寒性的一个棉花P型ATP酶基因Gbpatp的应用
WO2016095124A1 (en) 2014-12-17 2016-06-23 Kunming Institute Of Botany, The Chinese Academy Of Sciences Compositions and methods for increasing drought tolerance in plants

Also Published As

Publication number Publication date
EP3599829A4 (en) 2020-07-29
EP3599829A1 (en) 2020-02-05
US20230313179A1 (en) 2023-10-05
AU2024200329A1 (en) 2024-02-15
EP3599829B1 (en) 2023-10-04
MX2019011579A (es) 2020-01-20
US12054709B2 (en) 2024-08-06
US20240141329A1 (en) 2024-05-02
MX2023004224A (es) 2023-04-21
US20200115704A1 (en) 2020-04-16
US20210388340A1 (en) 2021-12-16
BR112019020167A2 (pt) 2020-06-02
WO2018178975A9 (en) 2018-11-29
US11111491B2 (en) 2021-09-07
CN110691509A (zh) 2020-01-14
ZA201906391B (en) 2020-09-30
AU2018242633A1 (en) 2019-10-17
ES2964023T3 (es) 2024-04-03
AU2018242633B2 (en) 2024-02-08
US20240209351A1 (en) 2024-06-27
PH12019502244A1 (en) 2020-06-29
US11905512B2 (en) 2024-02-20
CL2019002757A1 (es) 2020-03-06
EP3599829C0 (en) 2023-10-04
WO2018178975A1 (en) 2018-10-04
CA3057004A1 (en) 2018-10-04

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