CN110691509A - 用于改良植物性状的方法 - Google Patents

Abstract

本发明公开了一种用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：(a)从预定来源的采样获得遗传物质，以及(b)从所述遗传物质构建表达文库。前述方法还包括以下步骤：(c)产生用所述表达文库转化的植物，其转化效率为至少0.05％至30％，代表至少10²‑10¹⁰个转基因；(d)筛选表达所述期望性状的转化植物；和(e)鉴定所述转化植物的表达所述期望性状的所述转基因。

Description

用于改良植物性状的方法

序列表

本申请包含序列表，该序列表已经以ASCII txt格式电子方式提交，并且其全部内容通过引用并入本文。所述ASCII副本的名称为seq.listing 1754-P-01-PCT_ST25，大小为491KB。

技术领域

本发明一般涉及改良植物性状的领域。更具体地，本发明涉及通过将来自预定来源的表达文库转化到植物中并筛选期望的性状来改良植物的性状。

背景技术

到2050年，世界人口估计将达到92亿。要充分养活这一人口，粮食总产量必须增长60％-70％。气候模型预测，本世纪中的温暖温度以及干旱的频率和持续时间的增加将对农业生产率产生负面影响。例如，非洲的玉米生产可能面临重大减产的风险，因为研究人员预测，如果植物获得足够的水分，作物在高于30℃的温度下每花费一度日(degree-day)，产量就会降低1％。这些预测与美国玉米产量报道的类似。进一步表明，在干旱条件下，作物在高于30℃的温度下每花费一度日，非洲玉米产量就下降1.7％。在2010年，俄罗斯的小麦产量下降了近三分之一，这很大程度是是由于夏季热浪。同样，2010年中国和印度的小麦产量也显著下降，分别主要是由于干旱和温度突然升高从而造成了强制成熟。这些新的全球挑战需要更复杂的一体化农业。

此外，全球变暖导致许多非生物和生物胁迫同时存在，从而影响农业生产率。非生物胁迫的发生可以通过增强宿主植物对病原生物、昆虫的易感性以及降低与杂草的竞争能力来改变植物与害虫之间的相互作用。与之相反，一些害虫可能会改变植物对非生物胁迫因素的反应。

生物胁迫因素由病原体、昆虫、害虫、杂草或种内资源竞争引起。生物胁迫因素导致产量或质量损失的能力取决于环境，因此可能因地区而异，也可能因农业生态而异。例如，在澳大利亚，大麦叶病是导致大量产量和质量损失的一些主要生物胁迫因素。尽管已知一些植物物种对多种疾病具有抗性，但是这些植物物种很难或甚至不能以常规方法进行繁殖。

挑战是产生的作物对生物胁迫因素具有抗性并且灵活且适应多种环境和种群。当前有两种主要的使作物适应新环境的方法：通过常规育种开发新作物(从驯化开始的长期努力)和通过植物育种(包括基因工程)将靶性状引入现有作物。为了在气候变化加剧的情况下保持生产力，需要不断开发种群和植物品种以抵御“新的”极端气候和其它胁迫，例如疾病、病原体、昆虫、害虫等。此外，还对寻找对于新化学物质和新除草剂作用方式的新除草剂耐受性或抗性基因具有不断需求。

基因工程有潜力解决农民面临的通过单独常规植物育种不容易解决的一些最具挑战性的生物和非生物限制。

这些遗传修饰的有利结果包括增加食物生产、可靠性和产量；增强口味和营养价值；以及减少由于各种生物和非生物胁迫(例如真菌和细菌病原体)而导致的损失。这些目标持续激励着现代育种者和食品科学家，他们正在寻求更新的遗传修饰方法来鉴定、选择和分析具有遗传增强特征的个体生物。

选择用外来基因和/或来自很难或不可能繁殖的相同物种或属的基因转化植物克服了物种障碍，从而有可能开发无法通过常规方法获得的强大“超性状”。但是，引入的转基因和内源基因的分子相互作用和结果是不可预测的。

当转移编码某些性状的基因(通常是从一种植物物种到另一种)时，除非环境以预期方式与基因相互作用以触发期望反应，否则期望性状不会总是表达，这取决于插入有所述基因的调节序列。这意味着在实验室条件下在受控气候下开发的新转基因品种必须像与在更传统的育种方法中一样的田间条件下进行测试，因此目前两种方法在使新品种释放的速度中几乎没有差异。

从基础植物研究中获得的知识将为未来的作物改良奠定基础，但是将研究发现快速有效转化为公益性农业的有效机制仍有待开发。

美国专利6030779和美国专利6368798公开了一种用于鉴定具有指定酶活性的克隆的方法，其如下实现：使用多核苷酸探针从基因组DNA群体中选择性分离靶核酸，所述多核苷酸探针鉴定编码具有特定酶活性的酶的核酸序列；和用分离的靶核酸转化宿主以产生克隆文库，筛选克隆文库的指定酶活性。

美国专利6972183公开了一种用于筛选表达文库以鉴定表达具有期望活性的酶的克隆的方法。该方法涉及从一种或多种微生物的基因组DNA样品中产生包含多个重组细胞克隆的表达文库，然后向毛细管阵列中的毛细管中引入底物和克隆的子集。底物与表达具有期望活性的酶的克隆的相互作用产生光学可检测的信号，然后可对其进行空间检测以鉴定包含产生这种信号的克隆的毛细管。然后可以从鉴定的毛细管中回收产生信号的克隆。

欧洲专利申请1025262和美国专利申请20020150949教导了一种用于通过以下来鉴定具有指定目的活性的克隆的方法：(i)产生从直接分离自环境的核酸得到的表达文库；(ii)将所述文库暴露于特定的一种或多种目的底物；和(iii)利用荧光激活细胞分选来筛选所述经过暴露的文库以鉴定与一种或多种底物反应的克隆。

美国专利申请20100152051涉及一种用于鉴定和/或表征来自表达文库的赋予期望的生物学特性的克隆的方法。该方法包括以下步骤：筛选与所述表达文库的克隆的重组插入物一起表达为融合蛋白的至少一种(多)肽(例如标签)的表达。所述(多)肽可以在N末端或C末端与所述插入物融合。该方法还包括使与赋予所述期望生物学特性的克隆的插入物表达的(多)肽特异性相互作用的配体接触的步骤。

所有上述方法均基于通过与预定探针的相互作用筛选DNA文库(由微生物或环境样品产生)的特定序列或生化活性。另外，具有预定序列或活性的克隆的筛选和选择在转化入植物细胞之前进行，并且可以在植物细胞(组织培养物)中表达，但不能在整株植物中表达。因此，通过最新使用的方法，仅预先选定的克隆在植物中表达，并且所选序列在植物中的表达和作用是不可预测的。另外，在上述方法中，仅可以基于先验知识筛选已知活性。因此，这些方法被限制在已知酶活性和酶家族以及先前已知功能的范围内。

鉴于上述情况，长期以来一直需要用于筛选和鉴定赋予期望的植物改良性状的未知序列的有效方法。

发明内容

因此，本发明的一个目的是公开一种用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：(a)从预定来源的采样获得遗传物质；(b)从所述遗传物质构建表达文库；其中所述方法还包括以下步骤：(c)产生用所述表达文库转化的植物，转化效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；(d)筛选表达所述期望性状的转化植物；和(e)鉴定所述转化植物的表达所述期望性状的所述转基因。

本发明的另一个目的是公开如以上所限定的方法，该方法还包括根据从所述转基因获得的遗传信息编辑期望作物植物中的靶基因的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中使用任何基因组编辑系统或方法进行所述靶基因的所述编辑，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述预定来源包括环境生态位的植物、微生物、真菌或其它生物或其部分。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述筛选步骤包括与对照植物相比对所述转化植物的测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、植物地上部分鲜重、叶数、枝鲜重、主枝长度、花产量、荚或果实产量、缺绿症、叶损伤、植物的状态或性能及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述对照植物是与所述转基因植物相同属但是缺少所述转基因的植物，或者与所述转基因植物相同属、缺少所述转基因并且用赋予所述植物改良性状的已知基因转化的植物。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(a)还包括通过在富培养基或选择性培养基上生长来富集所述遗传物质的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(a)还包括通过在针对所述期望性状的选择性培养基上培养所述遗传物质来增强所述期望性状的表达的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(b)包括产生原核cDNA文库或真核cDNA文库或两者的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(b)还包括将所述cDNA文库克隆到至少一种双元载体中的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述双元载体包含组成型启动子或胁迫诱导型启动子。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述双元载体包含细菌选择标志物和植物转化选择标志物。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括将所述克隆的双元载体转化到宿主细胞中的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括将所述克隆的双元载体转化到根癌农杆菌(Agrobacterium tumefaciens)中的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括将所述转化的根癌农杆菌引入到以下至少一种中的步骤：整株植物、植物组织和植物细胞。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法包括通过向所述植物喷洒包含转化的农杆菌的接种物来引入所述转化的根癌农杆菌的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(d)包括在对所述期望性状具有选择性的条件下生长所述转化植物。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括以下步骤：(f)从步骤(d)的所述转化植物收集T1种子；(g)确定所述T1种子的种子文库转化效率；(h)在允许筛选和选择表达所述期望性状的转化植物的选择条件下播种步骤(e)的所述T1种子；(i)测试步骤(g)的所述选择的表达所述期望性状的植物中所述转基因的存在；和(j)对步骤(h)的对于所述转基因阳性测试的所述选择的转化植物的所述转基因进行分离和测序。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括以下步骤：(k)从(h)的发现对于所述转基因的存在为阳性的所述植物收集T2种子；(l)与用赋予所述期望性状的已知基因转化的对照植物相比，在允许筛选和选择表达所述期望性状的转化植物的选择条件下生长所述T2种子的植物；和(m)任选地，对步骤(j)的所述选择的植物的所述转基因进行分离和测序。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法包括以下步骤：(a)对步骤(i)和/或(l)的所述分离的转基因进行重克隆和测序；(b)将所述重克隆的转基因转化到植物中；(c)筛选步骤(b)的所述转化植物以选择表达所述期望性状的转化植物；(d)从步骤(c)的所述选择的植物中分离所述转基因；和(e)任选地，重复步骤(a)至(d)。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述环境生态位包括生态生态位、种群、生境、基因库、原核培养物、真核培养物及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述环境生态位包括微生物组、微生物群、微生物培养物、植物、酵母、藻类、线虫或任何其它生物或其组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述环境生态位包括预定的生物因子、非生物因子及其组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述采样包括土壤样品、水样品、有机物样品及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述期望性状选自由以下所组成的组：对至少一种生物胁迫的抗性或耐受性、对至少一种非生物胁迫的抗性或耐受性、提高的产量、提高的生物量、提高的食物品质和价值、提高的谷物产量、除草剂或化学物质抗性或耐受性及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述非生物胁迫选自由以下所组成的组：干旱、盐分、热、冷、肥料吸收、肥料使用效率及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述生物胁迫选自由以下所组成的组：植物疾病、病原体、细菌、病毒、真菌、寄生虫、有益和有害昆虫、杂草和栽培的或天然的植物或其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(a)包括从所述预定环境生态位的所述采样中提取RNA的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述RNA提取根据标准商业试剂盒或根据任何其它用于从环境采样中提取RNA的方案进行。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述用于从环境采样中提取RNA的方案包括以下步骤：(a)获得土壤样品；(b)将所述土壤样品与提取缓冲液混合，所述提取缓冲液包含500mM pH 8的磷酸盐缓冲液和5％w/v的鲸蜡基三甲基溴化铵(CTAB)以及比率为25:24:1的苯酚(pH 8)/氯仿/IAA；(c)使步骤(b)的所述混合物经受37℃下振摇15分钟或珠打浆机1分钟；(d)在室温下将步骤(c)的所述混合物在2500g下离心约10分钟以获得水相；(e)将所述水相转移到新管中；(f)向步骤(e)的所述管内的所述水相添加等量的补充有20mg/ml的结晶紫溶液的异丙醇，以获得紫色染色溶液；(g)通过倒转步骤(f)的所述管来混合所述溶液，然后将所述管在室温下孵育约30分钟；(h)在室温下将步骤(g)的所述管在2500g下离心约30分钟，以获得紫色染色层；(i)将所述紫色染色层转移到新管中，并将所述管以最大速度离心约5min，以获得沉淀和上清液；(j)用80％v/v冰冷的乙醇洗涤所述沉淀并且再离心5分钟，以获得沉淀和上清液；(k)除去步骤(j)的所述上清液并使所述沉淀干燥；和(l)将所述干燥的沉淀以100μl水比2克步骤(a)的土壤的比例混悬在水中。

本发明的另一个目的是公开一种植物，该植物包含通过以上任一者所限定的方法鉴定的所述转基因。

本发明的另一个目的是公开如以上所限定的植物，其中与缺少所述转基因的相同属的植物相比，所述植物具有至少一种植物改良性状。

本发明的另一个目的是公开可通过以上任一者所限定的方法获得的多核苷酸序列。

本发明的另一个目的是公开如以上任一者所限定的多核苷酸，其中所述多核苷酸包含与选自由SEQ ID NO:1-148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

本发明的另一个目的是公开多核苷酸序列，该多核苷酸序列与上述任一项所定义的多核苷酸序列具有至少80％序列相似性。

本发明的另一个目的是公开一种可通过以上任一者所限定的方法获得的多肽序列。

本发明的另一个目的是公开如以上任一者所限定的多肽序列，其中所述多肽包含与选自由SEQ ID NO:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

本发明的另一个目的是公开多肽序列，该多肽序列与以上任一者所限定的多肽序列具有至少60％序列相似性。

本发明的另一个目的是公开如以上任一者所限定的方法用于鉴定赋予植物改良性状的基因的用途，所述植物改良性状选自由以下所组成的组：对非生物胁迫的抗性或耐受性、对生物胁迫的抗性或耐受性、提高的产量、提高的生物量、提高的食物品质和价值、提高的谷物产量、除草剂或化学物质抗性或耐受性及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的用途，其中所述非生物胁迫选自由以下所组成的组：干旱、盐分、热、冷、肥料利用及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的用途，其中所述生物胁迫选自由以下所组成的组：植物疾病、病原体、细菌、病毒、真菌、寄生虫、有益和有害昆虫、杂草和栽培的或天然的植物或其任意组合。

本发明的另一个目的是公开一种用于筛选和鉴定植物中的干旱或盐分抗性或耐受性改良性状的方法，所述方法包括以下步骤：(a)获得来源于低水分或高盐分来源样品的遗传物质；(b)从所述遗传物质构建表达文库；其中所述方法还包括以下步骤：(c)产生用所述表达文库转化的植物，转化效率为至少0.5％至30％，代表至少10²-10¹⁰个转基因；(d)筛选对预定干旱或盐分条件具有抗性或耐受性的转化植物；以及(e)鉴定步骤(d)的所述干旱或盐分抗性或耐受性转化植物的所述转基因。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括根据从所述转基因获得的遗传信息编辑期望作物植物中的靶基因的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中使用任何基因组编辑系统或方法进行所述靶基因的所述编辑，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述预定来源包括环境生态位的植物、微生物、真菌或其它生物或其部分。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述筛选步骤包括与对照植物相比对所述转化植物的测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、植物地上部分鲜重、叶数、枝鲜重、主枝长度、花产量、荚或果实产量、缺绿症、叶损伤、植物的状态或性能及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述对照植物是与所述转基因植物相同属但是缺少所述转基因的植物，或者与所述转基因植物相同属、缺少所述转基因并且用赋予所述植物改良性状的已知基因转化的植物。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述步骤(b)还包括将所述表达文库克隆到至少一种双元载体中的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括以下步骤：(f)从步骤(c)的所述转化植物收集T1种子；(g)将所述T1种子播种在对转化植物有选择性的水含量为约100％容量的土壤中；(h)在干旱或盐分条件下和/或不灌溉的情况下生长所述T1种子的植物，直到大部分植物死亡，以产生在所述干旱或盐分条件下存活的转化植物；(i)使所述干旱或盐分存活转化植物生长以产生T2种子；(j)筛选步骤(i)的所述干旱或盐分存活转化植物中转基因的存在；和(k)从步骤(j)的阳性筛选植物分离所述转基因并且测序。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括以下步骤：(l)从步骤(j)的每一个所述含转基因的阳性筛选的干旱或盐分存活转化植物收集T2种子；(m)与相同属但缺少所述转基因或者用赋予干旱或盐分耐受性或干旱或盐分抗性的已知基因转化的对照植物相比，在预定干旱或盐分条件下从步骤(l)的每一个所述含转基因的T2种子生长T2植物；(n)与所述对照植物相比，对每一个所述含转基因的T2植物进行干旱耐受性或抗性筛选测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、鲜重、叶数、枝鲜重、主枝长度、花和荚产量、缺绿症和叶损伤、植物的状态或性能及其任意组合；(o)从步骤(n)的所述筛选的干旱或盐分抗性性能T2植物分离所述转基因；(p)任选地，将所述转基因重克隆到双元载体中；(q)任选地，将所述克隆的双元载体转化到植物中，并且在预定干旱或盐分条件下生长所述转化植物；和(r)任选地，重复步骤(l)至(q)。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述生长T2植物的步骤包括以下步骤：(a)将所述T2种子播种在对转化植物有选择性的水含量为约100％容量的土壤中；和(b)当所述土壤中的水含量达到约5-10％时，灌溉所述植物。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述预定干旱或盐分条件选自由以下所组成的组：低水分、高盐分、干燥土壤和热。

本发明的另一个目的是公开可通过以上任一者所限定的方法获得的多核苷酸序列。

本发明的另一个目的是公开如以上任一者所限定的多核苷酸，其中所述多核苷酸包含与选自由SEQ ID NO:1至SEQ ID NO:148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

本发明的另一个目的是公开多核苷酸序列，该多核苷酸序列与以上任一者所限定的多核苷酸序列具有至少80％序列相似性。

本发明的另一个目的是公开可通过以上任一者所限定的方法获得的多肽序列。

本发明的另一个目的是公开如以上任一者所限定的多肽序列，所述多肽包含与选自由SEQ ID NO:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

本发明的另一个目的是公开多肽序列，该多肽序列与以上任一者所限定的多肽序列具有至少60％序列相似性。

本发明的另一个目的是公开一种用于从土壤样品中提取RNA的方法，该方法包括以下步骤：(a)获得土壤样品；(b)将所述土壤样品与提取缓冲液混合，所述提取缓冲液包含500mM pH 8的磷酸盐缓冲液和5％w/v的鲸蜡基三甲基溴化铵(CTAB)以及比率为25:24:1的苯酚(pH 8)/氯仿/IAA；(c)使步骤(b)的所述混合物经受37℃下振摇15分钟或珠打浆机1分钟；(d)在室温下将步骤(c)的所述混合物在2500g下离心约10分钟以获得水相；(e)将所述水相转移到新管中；(f)向步骤(e)的所述管内的所述水相添加等量的补充有20mg/ml的结晶紫溶液的异丙醇，以获得紫色染色溶液；(g)通过倒转步骤(f)的所述管来混合所述溶液，然后将所述管在室温下孵育约30分钟；(h)在室温下将步骤(g)的所述管在2500g下离心约30分钟，以获得紫色染色层；(i)将所述紫色染色层转移到新管中，并将所述管以最大速度离心约5min，以获得沉淀和上清液；(j)用80％v/v冰冷的乙醇洗涤所述沉淀并且再离心5分钟，以获得沉淀和上清液；(k)除去步骤(j)的所述上清液并使所述沉淀干燥；和(l)将所述干燥的沉淀以100μl水比2克步骤(a)的土壤的比例混悬在水中。

本发明的另一个目的是公开一种用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：(a)获得预定来源的采样；(b)根据权利要求60所述的方法从所述采样提取RNA；(c)从步骤(b)的所述RNA构建表达文库；其中所述方法还包括以下步骤：(d)产生用所述表达文库转化的植物，效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；(e)筛选表达所述期望性状的转化植物；和(f)鉴定所述转化植物的表达所述期望性状的所述转基因。

本发明的另一个目的是公开如以上任一者所限定的方法，该方法还包括根据从所述转基因获得的遗传信息编辑期望作物植物中的靶基因的步骤。

本发明的另一个目的是公开如以上任一者所限定的方法，其中使用任何基因组编辑系统或方法进行所述靶基因的所述编辑，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述预定来源包括环境生态位的植物、微生物、真菌或其它生物或其部分。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述筛选步骤包括与对照植物相比对所述转化植物的测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、植物地上部分鲜重、叶数、枝鲜重、主枝长度、花产量、荚或果实产量、缺绿症、叶损伤、植物的状态或性能及其任意组合。

本发明的另一个目的是公开如以上任一者所限定的方法，其中所述对照植物是与所述转基因植物相同属但是缺少所述转基因的植物，或者与所述转基因植物相同属、缺少所述转基因并且用赋予所述植物改良性状的已知基因转化的植物。

本发明的另一个目的是公开分离的多核苷酸，该分离的多核苷酸与选自由SEQ IDNO:1至SEQ ID NO:148及其任意组合所组成的组的核苷酸序列具有至少80％序列相似性。

本发明的另一个目的是公开分离的多肽，该分离的多肽与选自由SEQ ID NO:149-321及其任意组合所组成的组的氨基酸序列具有至少60％序列相似性。

附图说明

为了理解本发明并看到其如何在实践中实施，现在将参照附图仅通过非限制性实例的方式描述多个实施例，其中：

图1A-D示出了用于在启动子(35S、CBF3、ErdlO和Kinl)和HSP终止子之间插入经扩增cDNA克隆的双元载体的示意图。图1A示出了具有组成型CaMV 35S启动子的pPA-35H载体。图1B-D示出了含有拟南芥(Arabidopsis thaliana)的胁迫诱导型启动子的载体：具有CBF3启动子的pPA-CH(图1B)，具有ErdlO启动子的pPA-EH(图1C)和具有Kinl启动子的pPA-KH(图1D)；

图2示出了对于LB培养皿上3个不同文库计数的农杆菌文库的照片；

图3示出了用文库转化的烟草组织培养物的照片：转化后7天(图3A)，转化后40天(图3B)和转化后6-8周(图3C)；

图4示出了说明选择10天龄的拟南芥表达文库幼苗的草丁膦抗性的照片。绿色植物对草丁膦有抗性，而小的黄色植物不存在转基因，因此是易感的。

图5示出了温室中T2和T3对照实验的照片；

图6示出了筛选对干旱具有抗性的转基因植物的照片结果；

图7示出了说明在停止灌溉后数天，表达用作对照的基因的植物中相性对于土壤含水量的膨压损失(深灰色)；

图8示出了显示与表达GFP的植物相比表达阳性对照的转基因植物的归一化死亡规模的图示；

图9图示了通过本发明的方法鉴定的多种干旱抗性基因的结果；以及

图10图示了与阳性和阴性对照相比表达鉴定的赋予干旱抗性的新基因的多种转基因植物品系在重克隆之后的叶面积分析。

具体实施方式

与本发明的所有章节一起提供了以下描述，以使本领域任何技术人员能够利用本发明，并阐明发明人想到的实施本发明的最佳方式。然而，由于本发明的一般原理已被特别限定以提供用于筛选和鉴定期望的植物改良性状的手段和方法，因此多种修改对本领域技术人员而言仍然是显而易见的。

已知一些植物物种对多种疾病具有抗性。但是，这样的物种通常很难或不可能通过常规技术和方法进行繁殖。

本发明提供了一种方法和平台，用于从植物中发现和鉴定具有独特且有价值的特征的基因，例如疾病抗性、非生物胁迫抗性或耐受性、食物改良品质(例如改良的油、蛋白质含量、氨基酸、维生素等)，然后通过基因编辑或其它转化技术在期望的作物中插入或表达它们。

因此，通过植物育种(包括基因工程和基因(基因组)编辑)将靶性状引入现有作物中在本发明的范围内。

本发明提供了一种用于筛选和鉴定期望的植物改良性状的新方法。所述方法包括以下步骤：(a)获得来自预定环境生态位的采样的遗传物质，或者提取自其它来源(例如相同或其它属的植物)的遗传物质；(b)从所述遗传物质构建表达文库。根据核心方面，本发明还包括以下步骤：(c)产生用所述表达文库转化的植物，效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；(d)筛选表达所述期望性状的转化植物；和(e)鉴定所述转化植物的表达所述期望性状的所述转基因。

本发明首次提供了一种用于筛选和选择来自在有价值的作物植物中赋予改良性状的预定来源(例如生态生态位和/或植物)的未知序列的方法。相对于普通和常规筛选方法，本发明方法在以下方面是有效和有利的：

1.表达文库是由源自预定来源例如极端环境、植物材料等的遗传物质或遗传库(即RNA)制备的。这样，仅将在预先选定环境条件下表达的基因用于植物中的筛选程序。

2.将整个表达文库转化到植物中，效率为0.05％-30％，并且代表至少10²-10¹⁰个独特的转基因。

3.在本发明的方法中，在作为植物的靶生物体中进行表达文库的期望表型的筛选。这样就没有预先选择，并且揭示了在植物中可表达的期望表型的新独特基因。

在常规方法中，第一步是在源遗传物质中选择用于预定性状的基因，例如通过在原核或真核细胞中探测具有已知序列的DNA文库，然后才在植物中表达预先选定的基因。这种常规方法的结果是有限的，并且具有以下缺点：

1.筛选是在不是靶生物体的宿主细胞/生物中(通常在原核或单细胞生物中)进行。

2.由于使用已知序列或探针或活性进行，因此筛选受到限制。已经发现，功能筛选方法需要可检测水平的酶活性，其无法始终实现，例如在基于大肠杆菌的表达系统中可能仅检测到约40％的酶活性(Gabor等人,2004)。此外，本文指出，尽管有可用的先进测序技术，但总蛋白质编码基因中有约35-60％显示出与“假设蛋白质”、“预测蛋白质”或“功能未知的蛋白质”的高度相似性(Culligan等人,2014；Venter等人,2004)。

3.仅将预先选定的克隆转化到植物中。

4.预先选定克隆在靶植物中的表达和作用是不可预测的。

由于上述原因，本发明筛选用表达文库转化的植物的期望表型的新方法是有利的。

本文承认干旱和盐分被认为是对植物生长和发育具有重要影响的两种非生物胁迫。

关于干旱，其被认为是最具有破坏性的环境胁迫，其降低作物的生长和生产力。干旱严重影响植物的生长和发育，导致生长速率和生物量积累显著降低。植物中干旱的主要后果是降低的细胞分裂和扩增的速率、叶片大小、茎伸长和根增殖、干扰气孔振幅以及水分利用效率(WUE)(Farooq等人.2009)。这种现象涉及遗传、生理和环境事件及其复杂的相互作用。植物生长的速度和量取决于这些事件，其受缺水影响。由于膨压和可用水的减少，细胞生长是对干旱最敏感的生理过程之一(Taiz和Zeiger,2006)。在缺水情况下，高等植物的细胞伸长可能被从木质部到周围伸长细胞的水流的中断而抑制。受损的有丝分裂、降低的细胞伸长和扩增导致植物高度、叶面积和作物生长的降低(Nonami,1998)。

盐分也被认为是影响作物生长和生产力的主要的严重非生物因素之一。在盐胁迫期间，所有主要过程(例如光合作用、蛋白质合成以及能量和脂质代谢)都会受到影响(Parida&Das,2005)。在最初暴露于盐分期间，植物会遭受水胁迫，从而减少叶片扩展。盐分胁迫的渗透影响可以在盐施用后立即观察到，并且据信在暴露期间会持续下去，从而导致受抑制的细胞扩增和细胞分裂以及气孔关闭。在长期暴露于盐分期间，植物经历离子胁迫，这可能导致成年叶片的过早衰老，从而减少可用于支持持续生长的光合面积。事实上，过量的钠以及更重要的氯化物可能不利地影响植物酶，导致能量产生减少和其它生理变化。进一步承认的是，离子胁迫导致较老的叶片过早衰老，以及成熟叶片中的毒性症状(缺绿症、坏死)。不希望受到理论的束缚，高钠离子通过破坏蛋白质合成并干扰酶的活性来影响植物(Carillo等人,2011)。

本发明提供了一种用于有效筛选在植物并且特别是在有价值的作物中赋予对干旱和/或盐分的抗性或提高的耐受性的新基因的方法。

本发明的方法通过使用表达的遗传物质(例如RNA或mRNA)(其代表例如由于环境条件(例如干旱或高盐)而在所选生物中表达的基因)并且产生代表某些生物生态位或其它遗传来源的“宏表达(Meta-Expression)”或宏转录组(metatranscriptome)状态的cDNA文库克服了上述缺点。然后将整个cDNA文库转化到植物中并且表达和筛选植物中期望的表型。

本发明的核心方面是从多种来源(包括植物)和环境产生表达文库。将表达文库转化到作为靶生物的植物中，以改善其性状或功能。然后筛选植物表达文库的期望性状，例如盐或干旱抗性或耐受性、提高的生物量和产量、生物胁迫(疾病和病原体)抗性或耐受性、提高的营养价值或提高的肥料利用率。

本文承认，植物生长于其中的环境(例如土壤)被微生物群落所栖息，例如一克土壤含有约10⁷-10⁹个微生物细胞(估计每克土壤中细菌物种的数量在2000至830万之间变化，https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2970868/)，其包含约一千兆碱基(gigabase)的序列信息，或更多。居住在植物生长环境(例如土壤)中的微生物群落非常复杂，并且尽管具有重要的经济意义，但仍然所知甚少。这样的微生物联盟(consortia)为植物生长提供了必要的生态系统，包括固定大气中的氮、营养循环、疾病抑制以及螯合铁和其它金属。

使用功能性宏基因组学和宏转录组学方法来探索赋予植物改良性状的新基因在本发明的范围内。

现在参考根据一些方面的由本发明所采用的宏基因组学方法。宏基因组学是对来自环境样品的遗传物质的研究。其通常指环境基因组学、生态基因组学或群落基因组学。尽管传统的微生物学和微生物基因组测序以及基因组学依赖于培养的克隆培养物，但是环境基因测序克隆特定基因以在天然样品中产生多样性谱。在一些方面，宏基因组学利用微生物群落中基因组的研究来构成研究微生物组的第一步。其的主要目的是推断微生物群落的分类学概况。全宏基因组测序(WMS)提供有关微生物群落功能概况的数据。这样的工作表明，通过基于培养的方法，错过了绝大多数微生物生物多样性。事实上，据估计，在地球上几乎每个环境中，超过99％的微生物无法在实验室中进行培养。

本文的宏基因组学还指代宏转录组学，其研究并关联一组相互作用的生物或物种的转录组。宏转录组学涉及对微生物群落的完整(宏)转录组进行测序。在一些方面，宏转录组学告知由整个群落表达的基因。通过使用表达基因的功能注释，可以推断特定条件下群落的功能概况，这通常取决于宿主的状态。尽管宏基因组学提供了在不同条件下的微生物群落组成的数据，而宏转录组学则提供了在不同条件下共同表达的基因的数据。宏转录组学涉及对整个群落的基因表达(RNA-seq)进行分析。在特定方面，宏转录组学描述了在特定微生物环境中表达的基因。因此，宏转录组学是对来自环境样品的完整转录本(RNA-seq)的功能和活性的研究。

应注意，基因表达呈对数状分布，例如，表达最高的前100个基因可覆盖所有转录本的多至30％。甚至单个基因也可覆盖10％的比例。因此，需要很高的测序深度才能看到表达水平较低的基因。

通过使用诸如“鸟枪”或PCR定向测序(PCR directed sequencing)的方法，可以从采样群落的成员中获得很大程度上无偏的基因样品。本文承认宏基因组学方法提供了利用微生物生态学来改善植物性状的强大工具，例如，可用于农业的生物学机制和改善的植物性状。

如本文所用，术语“约”表示所限定的量或量度或值的±25％。

如本文所用，术语“相似”表示约±20％、特别是±15％、更特别约±10％、并且甚至更特别约±5％的对应或相似范围。

如本文所用，术语“平均值”是指通过测量某一植物种群的每株植物中的预定参数并根据所述种群中植物的数目计算平均值而获得的平均值。

在本说明书和所附权利要求书中，除非上下文另外明确指出，否则未用数量词限定的名词指示一个/种或多个/种指示对象。因此，例如，提及“植物”时包括一个/种或多个/种植物，提及“性状”时包括一个/种或多个/种性状，提及“细胞”时包括细胞的混合物、组织等。

如本文所用，“植物”是指处于任何发育阶段的任何植物，包括植物种子。

术语“植物”包括整株植物或其任何部分或衍生物，例如植物细胞、植物原生质体、可从其再生植物的植物细胞组织培养物、植物愈合组织或愈伤组织、分生组织细胞、小孢子、胚、未成熟的胚、花粉、胚珠、花药、果实、花、叶、子叶、雌蕊、种子、种皮、根、根尖等。

本文使用的术语“植物细胞”是指植物的结构和生理单位，包含原生质体和细胞壁。植物细胞可以是分离的单细胞或培养的细胞的形式，或者作为较高组织单元例如植物组织、植物器官或整株植物的一部分。

如本文所用，术语“植物细胞培养物”或“组织培养物”是指植物单位的培养物，例如原生质体、可再生细胞、细胞培养物、细胞、植物组织中的细胞、花粉、花粉管、胚珠、胚囊、处于不同发育阶段的合子和胚、叶、根、根尖、花药、分生细胞、小孢子、花、子叶、雌蕊、果实、种子、种皮或其任意组合。

如本文所用，术语“植物材料”或“植物部分”是指植物的叶、茎、根、根尖、花或花部分、果实、花粉、卵细胞、合子、种子、种皮、插条、细胞或组织培养物或任何其它部分或产品或其任何组合。

如本文所用，“植物器官”是指植物的独特且可见的结构化和分化的部分，例如根、茎、叶、花、花芽或胚。

如本文所用，“植物组织”是指组织成结构和功能单元的一组植物细胞。包括植物中或培养物中的任何植物组织。该术语包括但不限于整株植物、植物器官、植物种子、组织培养物、原生质体、分生细胞、愈伤组织以及组织成结构和/或功能单元的任何一组植物细胞。该术语与以上所列或以其它方式由本定义所涵盖的任何特定类型的植物组织结合使用或在不存在后者时使用，并不旨在排除任何其它类型的植物组织。

如本文所用，术语“性状”是指特征或表型，尤其是指植物改良特征或表型。表型性状可以指个体的外观或其它可检测的特征，由其基因组、蛋白质组和/或代谢组与环境的相互作用而产生。例如，在本发明的上下文中，植物改良性状或期望的植物改良性状涉及对至少一种生物胁迫的抗性或耐受性、对至少一种非生物胁迫的抗性或耐受性、提高的产量或生物量、提高的谷物产量、提高的肥料吸收和使用效率及其任意组合。

性状可以显性或隐性方式或者以部分或不完全显性方式遗传。性状可以是单基因的(即由单个基因座决定)或多基因的(即由多于一个基因座决定)，或者也可以由一个或多个基因与环境的相互作用产生。显性性状导致在杂合或纯合状态时的完整表型表现。通常，隐性性状仅在以纯合状态存在时才表现出来。

在本发明的范围内，术语“表型”应理解为是指遗传控制性状的可区别特征。

如本文所用，短语“表型性状”是指个体的外观或其它可检测的特征，由其基因组、蛋白质组和/或代谢组与环境的相互作用产生。

在本发明的范围内，“胁迫”可以定义为对植物生长、功能和/或繁殖具有负面影响的任何外部因素。

术语“非生物胁迫”在本文中通常被定义为在特定环境中非生命因素对植物的负面影响。非生命变量必须以超出正常变化范围影响环境，以显著方式不利地影响植物或植物种群的性能或生理。非生物胁迫因素或胁迫源或环境因素的非限制性实例可包括诸如阳光、风、温度(冷、热)、盐分、过度浇水(洪水)、干旱等因素，以及诸如肥料吸收和肥料使用效率的因素，及其任意组合。非生物胁迫抗性或耐受性可以增强植物特别是作物的生长和生产力。已经表明，非生物胁迫因素是最有害的，并且当与非生物胁迫因素一起出现时，可能会产生协同效应。

术语“干旱”在下文中是指通常由长时间低于平均的降水或灌溉所引起的物理现象。例如，不足或低的水分(土壤或空气中)、供水短缺、干燥土壤、水分状况、高盐分、热及其任何组合。干燥条件可能由于不同的原因而形成。其可能对生态系统和农业产生重大影响，例如降低产量和作物损害。

许多生物具有干旱抗性生理和遗传适应性。

“生物胁迫”在本文中定义为由于其它活生物(例如细菌、病毒、真菌、粉虱、蓟马、蜘蛛、线虫、寄生虫、有益和有害昆虫、杂草以及栽培或原生植物)对植物造成的损害而产生的胁迫。施加在植物上的生物胁迫的类型可能取决于地理和气候两者以及寄主植物及其抵抗特定胁迫的能力。

如本文所用，词语“抗性”是指与相似环境条件下的易感植物相比，植物限制特定病原体的生长和发育和/或对植物造成的损害的能力。抗性植物在病原体或害虫压力下或在非生物胁迫条件下可能表现出某些疾病症状或损害。

进一步在本发明的范围内，抗性是指植物自身完全免疫特定的胁迫，例如生物营养性病原体感染。根据本发明的特定实施例，通过将表达文库转化至宿主植物，经转化的宿主获得了抗性基因，其阻止病原体的增殖和/或赋予对特定非生物胁迫(例如干旱)的抗性。

根据一些方面，抗性是绝对术语，其中植物自身完全免疫特定胁迫。应当指出，这并不意味着在生物或非生物胁迫下无法获得耐受性。

术语“耐受性”在下文中是指在通常会对相同物种的其它植物造成更大伤害的条件下，允许植物避免、耐受生物或非生物胁迫源或从其中恢复的植物特征。这些可遗传的特征影响对植物造成的损害程度。就农业生产的耐受性而言，意味着植物可处于胁迫(疾病/感染/或生理攻击)之下，但损失程度不超过经济阈值水平(损失程度不妨碍产品的经济潜力)。根据本发明的其它一些方面，耐受性是相对术语。耐受性的实例可存在于植物病原体和所有非生物胁迫的情况下，尤其是在由多个因素控制的复杂性状的情况下。

通常，“抗性”和“耐受性”是用来表示植物应对生物或非生物胁迫的能力的术语。

本文所用的术语“转化”是指通过将外源DNA引入细胞而引起的遗传改变或修饰。这包括将外源DNA整合进宿主基因组，和/或将包含外源DNA的质粒DNA引入植物细胞。这样的转化过程导致外源遗传物质(外源DNA，例如从生态生态位采样制备的表达文库)的摄取、并入和表达。植物转化可以是指利用分子和细胞生物学开发的直接或间接手段将外源基因引入植物细胞、组织或器官。

术语“环境生态位”或“生态生态位”通常是指生活在特定环境条件下的物种的行为。其包括居住于给定环境位置的微生物、真菌、植物或其它生物(极端微生物)。生态生态位描述了生物体或种群如何响应于资源和竞争者的分布，以及反过来又如何改变那些相同的因素。构成环境生态位规模的变量的类型和数量在物种之间变化，并且特定环境变量对于物种的相对重要性可能会根据地理非生物和生物环境而变化。

根据其它方面，术语“环境生态位”或“生态生态位”描述了物种或种群在生态系统中的关系位置。更具体地说，其描述了种群如何响应于其丰富的资源和竞争者以及如何影响这些相同的因素。非生物或物理环境也是生态位的一部分，因其影响种群如何影响资源和竞争以及如何被资源和竞争影响。生态位的描述可能包括生物体的生活史、生境以及在食物链中的位置的描述。在本发明的上下文中，“环境生态位”或“生态生态位”可以根据生物因素或非生物因素来定义，例如高盐分、干旱条件、高温、寒冷条件、pH或任何其它极端环境条件。

在本发明的范围内，遗传物质来自预定环境生态位的采样，包括来自土壤、水、植物生物质、微生物、酵母、藻类、线虫等。

如本文所用，术语“微生物组”或“微生物群”是指在从植物到动物的所有多细胞生物中和上发现的共生、共栖和致病微生物的生态群落。微生物群包括细菌、古细菌、原生生物、真菌和病毒。已经发现微生物群对于其宿主的免疫稳态、激素稳态和代谢稳态是至关重要的。同义术语微生物组描述了存在于环境生态位中的微生物的集体基因组或微生物本身。微生物组和宿主在进化过程中以表观遗传学和基因组特征的协同单位出现，有时统称为共生功能体(holobiont)。

术语“遗传物质”或“遗传库”在下文中是指给定时间种群的遗传物质的总和。其包括种群中的所有基因和基因组合(等位基因的总和)。

下文所用的术语“分离的”是指将材料从其原始环境(例如，如果是天然存在，则从自然环境)中去除。例如，从天然系统中的一些或全部共存物质分离的天然存在的多核苷酸或多肽是分离的。

从微生物或任何生物分离或来源的核酸可优选插入载体或质粒。这样的载体或质粒优选是包含表达调节序列(包括括适合在植物中表达的启动子、增强子等)的那些。在本文阐述的方案中详细描述了特别优选的质粒及其引入和转化的方法。

下文所用的术语“表达文库”是指包含以被宿主生物(在本发明的上下文中为植物)转录和/或翻译的方式构建的cDNA或基因组片段的代表性样品的载体或病毒(例如用作病毒载体的植物病毒)或质粒或噬菌体的集合。该技术使用表达载体产生克隆文库，每个克隆转录一种RNA和/或表达一种蛋白质。然后筛选该表达文库的目的特性，并且回收目的克隆以进行进一步分析。一个且非限制性的实例是使用表达文库分离可以赋予干旱抗性或耐受性的基因。

在本发明的范围内，可以将表达文库(通常来源于微生物遗传物质)构建在能够在多种宿主(例如大肠杆菌(E.coli)和根癌农杆菌(Agrobacterium tumefaciens))中复制以产生遗传修饰植物的双元载体(或转移DNA(T-DNA)双元系统或穿梭载体)中。这些是从根癌农杆菌中发现的天然存在的Ti质粒产生的人工载体。在一些方面，将表达文库从根癌农杆菌转移至植物。

术语“编辑”或“基因编辑”或“基因组编辑”在下文中是指任何常规或已知的基因组编辑系统或方法，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。在本发明的上下文中，根据从通过本发明的方法鉴定的转基因获得的信息，使用上述基因编辑技术来编辑期望作物植物中的靶基因。

术语“与序列相对应”在下文中指序列同源性或序列相似性。这些术语涉及两个或更多个核酸或蛋白质序列，其在用于最大对应性的比较和比对时是相同的或具有指定百分比的相同氨基酸残基或核苷酸，如使用一种可用的序列比较算法或通过目视检查测定的。

根据本发明的另一些方面，术语“与核苷酸序列相对应”是指所指示的核苷酸序列的变体、同源物和片段，其具有或执行相同的生物学功能或者与所指示核苷酸序列的相同表型特征相关。

两个核酸序列基本上相似或序列“与核苷酸序列相对应”的另一种指示是，两个分子在严格条件下彼此杂交。高严格条件(例如高杂交温度和杂交缓冲液中的低盐)仅允许高度相似的核酸序列之间的杂交，而低严格条件(例如较低温度和高盐条件)则允许当序列不太相似时进行杂交。

术语“相似性”或“序列相似性”在下文中是指当比较时两个序列之间的相似程度。这取决于它们的同一性，并且显示了残基被比对上的程度。序列相似性是指最佳匹配问题(即用于序列比对)。最佳匹配算法找到最少数量的编辑操作(插入、缺失和替换)，以便对一个序列与另一个序列进行比对。序列相似性检索可以通过检测过量相似性(即反映共同祖先的统计学上显著的相似性)来识别“同源”蛋白质或基因。

在本发明的范围内，相似性检索是用于鉴定同源物(即，具有共同的进化祖先的序列)的有效且可靠的策略或工具。相似性检索程序的非限制性实例包括BLAST(例如Altschul等人，1997)；3.3和3.4单元)、PSI-BLAST(例如Altschul等人，1997)、SSEARCH(例如Smith和Waterman，1981)；Pearson,1991，单元3.10)、FASTA(例如Pearson和Lipman，1988，单元3.9)和HMMER3(例如Johnson等人，2010)。这样的程序产生准确的统计学估计，并且可以确保具有显著相似性的蛋白质或核酸序列也可以具有相似的结构。相似性检索是有效且可靠的，因为可以推断出具有显著相似性的序列是同源的；即具有共同的祖先。

在本发明的范围内，相似性应理解为是指至少60％序列相似性，特别是至少70％，优选大于80％，更优选大于90％的相似性。术语“基本上相似”是指当比较整个ORF(开放阅读框)时与参考序列至少50％相同的核酸，其中序列相似性优选为至少70％，更优选至少80％，还更优选至少85％，特别是大于约90％，最优选95％或更大，特别是98％或更大。

在本发明的一些实施例中，这样的基本相似的序列是指与指示的多核苷酸或氨基酸序列具有至少约60％相似性，优选地至少约80％相似性，或者约90％、95％、96％、97％、98％或99％相似性的多核苷酸或氨基酸序列。

本发明涵盖与通过本发明的方法鉴定的多核苷酸序列或与参考序列具有至少60％相似性，优选70％，更优选80％，甚至更优选90％并且特别更优选95％相似性的核苷酸序列。

本发明还涵盖与通过本发明的方法鉴定的多肽序列或与参考序列具有至少60％相似性，优选70％，更优选80％，甚至更优选90％并且特别更优选95％相似性的氨基酸序列。

如本文所用，“参考序列”是用作序列比较的基础的限定序列。参考序列可以是指定序列的子集或整体；例如，作为全长cDNA或基因序列的片段，或完整的cDNA或基因或蛋白质序列。

如本文所用，在两个核酸或多肽序列情况下的“序列同一性”或“同一性”是指当在指定比较窗口内进行最大对应性比对时两个序列中相同的残基。当使用序列同一性百分比来表示蛋白质时，认识到不相同的残基位置通常会因保守的氨基酸取代而有所不同，其中氨基酸残基被具有类似化学性质(例如，电荷或疏水性)的其它氨基酸残基取代，因此不会改变分子的功能特性。

术语“同一性”或“序列同一性”在下文中还指在两个不同序列之间精确匹配的特征的量。因此，不计算缺口(gap)，并且测量与两个序列中较短的一个有关。

换句话说，如果相互比较的两个序列的长度不同，则序列同一性优选地涉及较短序列中与较长序列的核苷酸残基相同的核苷酸残基的百分比。如本文所用，在考虑需要被引入以用于两个序列最佳比对的缺口的数目和每个缺口的长度的情况下，两个序列之间的同一性百分比是两个序列具有的相同位置的数目的百分比。可以使用相关领域中已知的数学算法来完成序列的比较和两个序列之间的同一性百分比的确定。

进一步在范围内，术语“相似性”和“同一性”还指局部同源性，鉴定了同源或相似(在核苷酸和/或氨基酸序列中)的结构域。公认的是，诸如BLAST、SSEARCH、FASTA和HMMER的生物信息学工具计算局部序列比对，其鉴定两个序列之间最相似的区域。对于在不同蛋白质中不同序列背景下发现的结构域，比对应限于同源结构域，因为结构域同源性提供了得分中捕获的序列相似性。根据一些方面，术语相似性或同一性还包括序列基序，其是广泛的并且具有或被认为具有生物学意义的核苷酸或氨基酸序列模式。蛋白质可以具有序列基序和/或结构基序，该基序是由可能不相邻的氨基酸的三维排列形成的。

根据另一些实施例，通过本发明的方法鉴定了具有特定位置或结构域序列相似性的蛋白质或多核苷酸序列。当比较无保守性的残基时，低相似性是没有意义的，因此在保守区域中具有较高保守性的较低总体相似性序列在给定范围在被认为是相似的，例如在基于hmm的搜索算法(例如HMMER3)可以发现的>60％(即，与最接近的同源序列显示约37％的低相似性但是具有特定蛋白家族的所有保守底物结合残基的序列)。

术语“保守结构域数据库(CDD)”是指代表蛋白质结构域的序列比对和谱的集合。其还包括结构域与数据库(即分子模型数据库(MMDB))中已知的3维蛋白质结构进行比对。

在本发明的一些实施例中，这样的基本相同的序列是指与指定的多核苷酸或氨基酸序列具有至少约60％同一性，优选至少约80％的同一性，或者约90％、95％、96％、97％、98％或99％同一性的多核苷酸或氨基酸序列。

在本发明范围内的多肽与通过本发明方法鉴定的蛋白质具有至少50％同一性；或者与通过本发明方法鉴定的蛋白质或与参考序列具有至少55％同一性、或至少60％同一性、或至少65％同一性、或至少70％同一性、或至少75％同一性、或至少80％同一性、或至少85％同一性或至少90％同一性或至少90％同一性。

如本文所用，“序列同一性百分比”是指通过比较在比较窗口中最佳比对的两个序列而确定的值，其中与参考序列(不包含添加或缺失)相比，比较窗口中的多核苷酸序列的部分可包含添加或缺失(即缺口)，以用于两个序列的最佳比对。如下计算百分比：确定两个序列中出现相同核酸碱基或氨基酸残基的位置的数目以产生匹配位置的数目，用匹配位置的数目除以比较窗口中位置的总数目，并且将结果乘以100以产生序列同一性百分比。

术语多核苷酸序列的“基本同一性”是指使用标准参数描述的比对程序之一，多核苷酸序列包含与参考序列相比包含至少80％序列同一性，优选至少85％、更优选至少90％、最优选至少95％序列同一性。本领域技术人员将认识到，通过考虑密码子简并性、氨基酸相似性、阅读框定位等，可以适当地调节这些值以确定由两个核苷酸序列编码的蛋白质的相应同一性。为了这些目的，氨基酸序列的基本同一性通常是指至少80％，优选至少85％，更优选至少90％，最优选至少95％的序列同一性。优选地，使用Needleman等人的同源性比对算法进行最佳比对(1970.J.Mol.Biol.48:443)。

如本文所用，术语“同源物”是指在共有的氨基酸或核苷酸序列方面具有一定程度的序列相似性的DNA或氨基酸序列。可能存在部分相似性或完全相似性(即同一性)。对于蛋白质序列，可以使用氨基酸相似性矩阵，这在不同的生物信息学程序中是已知的(例如BLAST、FASTA,Bestfit program-Wisconsin Sequence Analysis Package,Version 8forUnix,Genetics Computer Group,University Research Park,575Science DriveMadison,WI 53711,Smith Waterman)。当利用不同的矩阵执行特定检索时，可能获得不同的结果。如本领域所公知的，核苷酸序列的相似性程度是基于同一性匹配，其中具有对优化比对所需的缺口或插入所作出的罚分(例如，Altschul S.F.等人,1990,J Mol Biol 215(3):403-10；Altschul S.F.等,1997,Nucleic Acids Res.25:3389-3402)。可以使用本领域周知的计算机程序(例如DNASTAR软件)找到确定哪些氨基酸残基可以被取代、插入或缺失而不丧失生物学或活性的指南。

在本发明的范围内，术语“多态性”应理解为是指群体中存在两种或更多种不同形式的基因、遗传标志物或遗传性状或基因产物，其可例如通过选择性剪接、DNA甲基化等获得。

本发明涵盖“高通量筛选”或“HTS”技术，其在本文中是指快速鉴定调节特定生物分子途径或功能的基因的方法。其包括宏转录组学和宏基因组学基因表达。

本发明概述了由从生态生态位中分离的遗传物质产生表达文库的方法，所述表达文库可以转化到靶植物中以用于筛选期望性状，例如对生物或非生物胁迫的耐受性或抗性以及提高产量或生物质生产。

根据一个实施例，本发明提供了一种用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：(a)从预定环境生态位的采样获得遗传物质；和(b)从所述遗传物质构建表达文库。根据核心实施例，本发明还包括以下步骤：(c)产生用表达文库转化的植物，效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因，从而在植物或种子中产生表达文库；(d)筛选表达期望性状的转化植物；和(e)鉴定转化植物的表达期望性状的转基因。

范围内还公开了如以上任一者所限定的方法，其中步骤(a)还包括通过在富培养基或选择性培养基上生长来富集遗传物质的步骤。

范围内还公开了如以上任一者所限定的方法，其中步骤(a)还包括通过在针对期望性状的选择性培养基上培养遗传物质来增强期望性状的表达的步骤。

范围内还公开了如以上任一者所限定的方法，其中步骤(b)包括产生原核cDNA文库或真核cDNA文库或两者的步骤。

范围内还公开了如以上任一者所限定的方法，其中步骤(b)还包括将cDNA文库克隆到至少一种双元载体中的步骤。

范围内还公开了如以上任一者所限定的方法，其中所述双元载体包含组成型启动子或胁迫诱导型启动子。

范围内还公开了如以上任一者所限定的方法，其中所述双元载体包含细菌选择标志物和植物转化选择标志物。

范围内还公开了如以上任一者所限定的方法，其中细菌选择标志物是卡那霉素抗性或任何其它抗生素抗性赋予基因，而植物转化选择标志物是赋予对包含草丁膦的除草剂(例如Basta除草剂)的抗性的bar基因。

现在提及草铵膦(也称为草丁膦，通常是铵盐)是由链霉菌属土壤细菌的多个物种产生的天然存在的广谱内吸性除草剂。草铵膦是一种用于控制杂草的广谱除草剂。其以包括Basta、Rely、Finale、Challenge和Liberty的品牌的制剂出售。bar基因赋予对除草剂Basta(含草丁膦)的抗性。

范围内还公开了如以上任一者所限定的方法，还包括将克隆的双元载体转化到宿主细胞中的步骤。

范围内还公开了如以上任一者所限定的方法，还包括将克隆的双元载体转化到根癌农杆菌中的步骤。

范围内还公开了如以上任一者所限定的方法，还包括将转化的根癌农杆菌引入到以下至少一种中的步骤：整株植物、植物组织和植物细胞。

范围内还公开了如以上任一者所限定的方法，包括通过向植物喷洒包含转化的农杆菌的接种物来引入转化的根癌农杆菌的步骤。

范围内还公开了如以上任一者所限定的方法，其中步骤(d)包括在对期望形状具有选择性的条件下培养转化植物。

范围内还公开了如以上任一者所限定的方法，还包括以下步骤：

f.从步骤(d)的转化植物收集T1种子；

g.通过计算草丁膦抗性植物与植物总数的比率，确定T1种子的种子文库效率；

h.在允许筛选和选择表达期望性状的转化植物的选择性条件下播种步骤(e)的T1种子；

i.测试步骤(g)选择的的表达期望性状的植物中转基因的存在；和

j.对步骤(h)的对于转基因阳性测试的选择的转化植物的转基因进行分离和测序。

范围内还公开了如以上任一者所限定的方法，还包括以下步骤：

k.从(h)的发现对于转基因的存在为阳性的植物收集T2种子；

l.与用赋予期望性状的已知基因转化的对照植物相比，在允许筛选和选择表达期望性状的转化植物的选择性条件下生长T2种子的植物；和

m.任选地，对步骤j的选择的植物的转基因进行分离和测序。

范围内还公开了如以上任一者所限定的方法，还包括以下步骤：

a.对步骤(i)和/或(l)的分离的转基因进行重克隆和测序；

b.将重克隆的转基因转化到植物中；

c.筛选步骤(b)的转化植物以选择表达期望性状的转化植物；

d.从步骤(c)的选择的植物中分离转基因；和

e.任选地，重复步骤(a)至(d)。

范围内还公开了如以上任一者所限定的方法，其中环境生态位包括来源于生态生态位、来源、种群、生境、基因库、原核培养物、真核培养物及其任何组合的样品。

范围内还公开了如以上任一者所限定的方法，其中环境生态位采样包括微生物组、微生物群或微生物培养物、植物、酵母、藻类、线虫或任何其它生物或其组合。

范围内还公开了如以上任一者所限定的方法，其中根据生物因子、非生物因子及其组合来限定环境生态位。

范围内还公开了如以上任一者所限定的方法，其中环境生态位采样包括土壤样品、水样品、有机物样品、任何活生物体(例如植物、酵母、细菌、微生物、藻类、线虫)及其任意组合。

范围内还公开了如以上任一者所限定的方法，其中所述期望性状选自由以下所组成的组：对至少一种生物胁迫的抗性或耐受性、对至少一种非生物胁迫的抗性或耐受性、提高的产量或生物量、提高的谷物产量、提高的肥料吸收和提高的使用效率及其组合。

范围内还公开了如以上任一者所限定的方法，其中非生物胁迫选自由以下所组成的组：干旱、盐分、热、冷、肥料吸收、肥料利用效率及其任意组合。

范围内还公开了如以上任一者所限定的方法，其中生物胁迫选自由以下所组成的组：病原体、细菌、病毒、真菌、寄生虫、有益和有害昆虫、杂草和栽培的或天然的植物或其任意组合。

范围内还公开了如以上任一者所限定的方法，其中所述方法包括从预定环境生态位的采样中提取RNA的步骤。

范围内还公开了如以上任一者所限定的方法，其中RNA提取根据标准商业试剂盒或根据任何其它用于从环境采样中提取RNA的方案进行。

范围内还公开了如以上任一者所限定的方法，其中用于从环境采样中提取RNA的方案包括以下步骤：

a.获得土壤样品；

b.将土壤样品与提取缓冲液混合，所述提取缓冲液包含500mM pH 8的磷酸盐缓冲液和5％w/v的鲸蜡基三甲基溴化铵(CTAB)以及比率为25:24:1的苯酚(pH 8)/氯仿/IAA；

c.使步骤(b)的混合物经受37℃下振摇15分钟或珠打浆机1分钟；

d.在室温下将步骤(c)的混合物在2500g下离心约10分钟以获得水相；

e.将水相转移到新管中；

f.向步骤(e)的管内的水相中添加等量的补充有20mg/ml的结晶紫溶液的异丙醇，以获得紫色染色溶液；

g.通过倒转步骤(f)的管来混合所述溶液，然后将管在室温下孵育约30分钟；

h.在室温下将步骤(g)的管在2500g下离心约30分钟，以获得紫色染色层；

i.紫色染色层转移到新管中，并将管以最大速度离心约5min，以获得沉淀和上清液；

j.用80％v/v冰冷的乙醇洗涤所述沉淀并且再离心5分钟，以获得沉淀和上清液；

k.除去步骤(j)的上清液并使沉淀干燥；和

l.将干燥的沉淀以100μl水比2克步骤(a)的土壤的比例混悬在水中。

范围内还公开了可通过如以上所限定的方法获得的多核苷酸序列。

范围内还公开了如以上所限定的多核苷酸，其中所述多核苷酸包含与选自由SEQIDNO:1-148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

范围内还公开了与可通过如以上所限定的方法获得的多核苷酸序列具有至少80％、85％、90％或95％序列相似性的多核苷酸序列。

范围内还公开了可通过如以上所限定的方法获得的多肽序列。

范围内还公开了如以上所限定的多肽序列，其中所述多肽包含与选自由SEQ IDNO:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

范围内还公开了与可通过如以上所限定的方法获得的氨基酸具有至少60％、70％、80％或90％序列相似性的氨基酸序列。

范围内还公开了如以上所限定的方法在用于鉴定赋予对非生物或生物胁迫的抗性或耐受性的基因中的用途。

范围内还公开了如以上所限定的方法在用于鉴定在暴露或不暴露于胁迫条件下赋予植物提高的产量和生物量(即提高的谷物产量，例如通过增强生长)的基因中的用途。

范围内还公开了如以上所限定的方法在用于鉴定赋予提高的产量的基因中的用途。

范围内还公开了如以上任一者所限定的用途，其中所述非生物胁迫选自由以下所组成的组：干旱、盐分、热、冷、肥料利用、肥料吸收及其任意组合。

范围内还公开了如以上任一者所限定的用途，其中生物胁迫选自由以下所组成的组：病原体、细菌、病毒、真菌、寄生虫、有益和有害昆虫、杂草和栽培的或天然的植物或其任意组合。

范围内还公开了用于筛选和鉴定植物中的干旱抗性或耐受性改良性状的方法，所述方法包括以下步骤：(a)获得来源于低水分或高盐分环境生态位样品的遗传物质；和(b)从所述遗传物质构建表达文库。根据核心实施例，所述方法还包括以下步骤：(c)产生用所述表达文库转化的植物，效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；(d)筛选在预定干旱条件下存活的转化植物；和(e)鉴定步骤(d)的干旱存活的转化植物的转基因。

范围内还公开了如以上所限定的方法，其中步骤(b)还包括将表达文库克隆到至少一种双元载体中的步骤。

范围内还公开了如以上任一者所限定的方法，还包括以下步骤：

f.从步骤(c)的转化植物收集T1种子；

g.将所述T1种子播种在对转化植物有选择性的水含量为约100％容量的土壤中；

h.在干旱条件和/或不灌溉的情况下生长T1种子的植物，直到大部分植物死亡，以产生在干旱条件下存活的转化植物；

i.使干旱存活的转化植物生长以产生T2种子；

j.筛选步骤(i)的干旱存活的转化植物中转基因的存在；

k.从步骤(j)的阳性筛选植物分离转基因并且测序。

范围内还公开了如以上任一者所限定的方法，还包括以下步骤：

l.从步骤(j)的每一个含转基因的阳性筛选的干旱存活转化植物收集T2种子；

m.与用赋予干旱耐受性或干旱抗性的已知基因转化的对照植物相比，在预定的干旱条件下从步骤(l)的每一个含转基因的T2种子生长T2植物；

n.与对照植物相比，对每一个含转基因的T2植物进行干旱耐受性或抗性筛选测量，其选自由以下所组成的：膨压测量、植物死亡数、植物状态及其任意组合；

o.从步骤(n)的筛选的干旱抗性性能T2植物分离转基因；

p.任选地，将转基因重克隆到双元载体中；

q.任选地，将克隆的双元载体转化到植物中，并且在预定干旱条件下生长转化植物；和

r.任选地，重复步骤(l)至(q)。

范围内还公开了如以上任一者所限定的方法，其中生长T2植物的步骤包括以下步骤：(a)将T2种子播种在对转化植物有选择性的水含量为约100％容量的土壤中；和(b)当土壤中的水含量达到约5-10％时，灌溉所述植物。

范围内还公开了如以上任一者所限定的方法，其中预定干旱条件选自低水分、高盐分、干燥土壤和热。

范围内还公开了可通过如以上任一者所限定的方法获得的多核苷酸序列。

范围内还公开了如以上所限定的多核苷酸，其中所述多核苷酸包含与选自由SEQIDNO:1-148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

范围内还公开了与可通过如以上任一者所限定的方法获得的多核苷酸序列具有至少80％、85％、90％或95％序列相似性的多核苷酸序列。

范围内还公开了可通过如以上任一者所限定的方法获得的多肽序列。

范围内还公开了如以上所限定的多肽序列，其中所述多肽包含与选自由SEQ IDNO:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

范围内还公开了与可通过如以上任一者所限定的方法获得的氨基酸序列具有至少60％、70％、80％或90％序列相似性的多肽序列。

本发明的范围内还公开了用于从土壤样品中提取RNA的方法，包括以下步骤：

m.获得土壤样品；

n.将所述土壤样品与提取缓冲液混合，所述提取缓冲液包含500mM pH 8的磷酸盐缓冲液和5％w/v的鲸蜡基三甲基溴化铵(CTAB)以及比率为25:24:1的苯酚(pH 8)/氯仿/IAA；

o.使步骤(b)的所述混合物经受37℃下振摇15分钟或珠打浆机1分钟；

p.在室温下将步骤(c)的所述混合物在2500g下离心约10分钟以获得水相；

q.将所述水相转移到新管中；

r.向步骤(e)的所述管内的所述水相添加等量的补充有20mg/ml的结晶紫溶液的异丙醇，以获得紫色染色溶液；

s.通过倒转步骤(f)的所述管来混合所述溶液，然后将所述管在室温下孵育约30分钟；

t.在室温下将步骤(g)的所述管在2500g下离心约30分钟，以获得紫色染色层；

u.将步骤(h)的所述紫色染色层转移到新管中，并将所述管以最大速度离心约5min，以获得沉淀和上清液；

v.用80％v/v冰冷的乙醇洗涤所述沉淀并且再离心5分钟，以获得沉淀和上清液；

w.除去步骤(j)的所述上清液并使所述沉淀干燥；和

x.将所述干燥的沉淀以100μl水比2克步骤(a)的土壤的比例混悬在水中。

本发明的范围内还公开了用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：

y.获得预定义环境生态位的采样；

z.根据如上文所限定的用于从土壤样品中提取RNA的方法从采样中提取RNA；

aa.从步骤(b)的RNA构建表达文库；

所述方法还包括以下步骤：

bb.产生用所述表达文库转化的植物，效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；

cc.筛选表达期望性状的转化植物；和

dd.鉴定表达期望性状的转化植物的转基因。

本发明的范围内还公开了分离的多核苷酸，该分离的多核苷酸具有与选自由SEQIDNO:1至SEQ ID NO:148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

本发明的范围内还公开了分离的多肽，该分离的多肽具有与选自由SEQ.ID No:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

为了理解本发明并且看到其如何在实践中实现，现在将仅通过非限制性实例的方式，参考以下实来描述多个优选实施例。

实例1

通过将来自生态生态位的表达文库转化到植物中并且筛选期望性状来改良植物性状的方法

1.样品采集与处理

第一步，已分离了来自多种环境样品和来源(例如来自不同生境的土壤、水或有机物)的遗传库。根据特定的期望目标性状选择来源。例如，当筛选干旱或盐分抗性基因时，将使用干旱土地，例如沙漠土地或高盐分土地或其它实施，但不是必须的。

每个样品中发现的微生物组可任选地通过在富培养基上生长或通过用抗生素选择性生长来富集。为了增强潜在期望基因的表达，使培养物在类似于、与目标性状相关或影响目标性状的胁迫条件或培养基中生长，例如对于干旱或盐分抗性性状的富含盐或PEG的培养基。

在振动培养箱中于28℃-37℃下，在富生长培养基(例如YPD)上进行样品富集数天。如果制备真核文库，则添加抗细菌抗生素，例如青霉素-链霉素和壮观霉素。

为了诱导胁迫抗性基因，使样品在任何期望的环境胁迫条件下生长。例如，为了诱导干旱抗性基因，通过在生长培养基中添加PEG(10％-30％w/v)使样品在高渗透压下生长。高盐浓度培养基例如NaCl(5％-10％w/v)用于诱导高盐分胁迫。此外，使样品暴露于不同的氮浓度(补充有6mM KH₂PO₄和微量元素的水中的0-100mM KNO₃，参见表1，制造商建议http://www.gatfertilizers.com/properties-of-solid-and-liquid-fertilizers/)、极端温度(50-60℃)和任何期望的环境胁迫。

表1：

2.RNA提取

已经根据标准商业试剂盒例如RNeasy PowerSoil Total RNA Kit(Qiagen)和Quick-RNA(Zymo research)进行了总RNA提取。另外，如下所示将独特方案用于从土壤样品中提取RNA：

在7ml的管中，用提取缓冲液(500mM pH8的磷酸盐缓冲液和5％w/v CTAB以及苯酚(pH 8)、氯仿、IAA(25:24:1))瓦解2g土壤。使管经受37℃下振摇15分钟或珠打浆机1分钟。然后将管在室温下以2500g离心10分钟。将水相转移到新管中，并添加等量的补充5ul结晶紫溶液(20mg/ml)异丙醇。通过颠倒将管混合，在室温下静置30分钟，然后在室温下以2500g离心30分钟。将紫色染色层转移到新的1.5ml管中，并以最大速度离心5分钟。将沉淀用500μl 80％v/v冰冷的乙醇洗涤并且再离心5分钟。离心之后，除去液体，并将沉淀干燥。将干燥的沉淀物混悬在100μl水中

3.cDNA文库的构建

3.1.真核cDNA文库

基于模板切换——poly-A mRNA的逆转录(SMART)或cDNA末端的低封端快速扩增(5'-RACE)方法，构建了来自总RNA和mRNA的真核cDNA文库。所用的poly-A mRNA逆转录引物为5’-ATTCTAGAGCGATCGCACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’(称为SEQ.ID NO:321)和5’-AAGCAGTGGTATCAACGCAGAGTGGCGCGCCrGrGG-3’(称为SEQ.ID NO:322)。cDNA末端的低封端快速扩增引物为5’-ATTCTAGAGCGATCGCACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’(称为SEQ ID NO:321)和5’-InvddT(5'反向二脱氧-T)-r(AAGCAGUGGUAUCAACGCAGAGUGGCGCGCCG)-3’(称为SEQ ID NO:323)。将扩增的cDNA在启动子(35S、KINl、erdlO和/或CBF3)和HSP或NOS终止子之间插入双元载体(见图1-4)中。图1A示出了pPA-35H载体，其具有组成型CaMV 35S启动子，其中GFP基因作为示例克隆在启动子和终止子之间。图1B-D示出了含有拟南芥(Arabidopsis thaliana)的胁迫诱导型启动子的载体：具有CBF3启动子的pPA-CH，其具有如SEQ.ID NO:330中所示的核苷酸序列(图1B)，具有ErdlO启动子的pPA-EH，其具有如SEQ.ID NO:331中所示的核苷酸序列(图1C)和具有Kinl启动子的pPA-KH，其具有如SEQ.ID NO:332中所示的核苷酸序列(图1D)，其中GFP基因作为示例克隆在启动子和终止子之间(Plant Physiol.1997Oct；115(2):327–334.,Plant Journal(2004)38,982-993，通过引用并入本文)。

这些载体包含卡那霉素作为细菌选择和bar基因作为赋予对草丁膦除草剂的抗性的转基因植物选择。使用吉布森组装(Gibson assembly)、限制性连接、无限制性或融合法中的至少一种非限制性实例，然后将连接产物转化到大肠杆菌感受态细胞中以在卡那霉素的选择下生长。文库的大小通过LB培养皿上接种的转化细菌的活菌计数来估计(通常为10^5–10^7)(图5)。使用标准微型制备试剂盒从大肠杆菌中纯化cDNA文库的载体，并且转化到电感受态(electrocompetent)根癌农杆菌GV3101细胞中。转化的农杆菌在LB培养基上在卡那霉素和利福平选择(每种50μg/ml)下于28℃生长过夜(每1m²目标植物生长面积250ml)。在冰上使生长停止至少30分钟，然后在4℃下以8000rpm离心10分钟。将沉淀的农杆菌混悬于混悬缓冲液(5％蔗糖和0.03％L-77Silwet,Momentive,US)中。

3.2.原核生物cDNA文库

使用ScriptSeq^TMComplete Kit(epicenter)基于标准5’和3’RNA修饰构建来自总RNA的原核生物cDNA文库。使用的引物为5’-ATTCTAGAGCGATCGCACATGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3’(称为SEQ.ID NO：321)和5’-InvddT(5'反向二脱氧-T)-r(AAGCAGUGGUAUCAACGCAGAGUGGCGCGCCG)-3’(称为SEQ ID NO:324)。将经扩增的cDNA插入具有卡那霉素和草丁膦抗性的运载载体中，然后转化到大肠杆菌感受态细胞中，以在卡那霉素选择(50μg/ml)下生长。文库大小通过转化宿主的活体计数来估计(通常为10^5–10^7)。使用标准微型制备试剂盒(50μl)从宿主细胞中纯化cDNA文库的载体，并且转化到电感受态农杆菌GV3103细胞(100μl)中。转化的农杆菌在LB培养基上在卡那霉素和利福平选择(50μg/ml)下于28℃生长过夜(每1m²目标植物生长面积100ml)。在冰上使生长停止至少30分钟，然后在4℃下以8000rpm离心10分钟。将沉淀的农杆菌混悬于混悬缓冲液(5％蔗糖和0.03％L-77Silwet)中。

4.植物的生长和转化

4.1.拟南芥植物

使植物生长在受控温室中作为转化的准备。使植物生长在由75％的泥炭、25％的珍珠岩组成的土壤中，并且需要时根据制造商说明定期用补充有肥料(例如，Shefer5.3.8,ICL Israel)的水灌溉。植物在3-4周之后开始开花，然后可以进行转化。使利用表达文库的转化农杆菌如上所述生长，并混悬在混悬缓冲液(5％蔗糖和0.03％L-77Silwet)中，并用2升喷雾器(例如Solo,German)喷在花上。在植物持续生长5-6周后变干时，收集种子并将其在阴凉干燥的地方放置2周或直到使用。

4.2.烟草植物

将烟叶切成1-2cm²的片，并用70％乙醇然后用0.3％漂白剂处理5分钟来灭菌。将叶片与文库转化的农杆菌(或与表4中SEQ ID 1-148的任一已鉴定基因)混合，混悬在补充有MS的液体再生培养基(RM)中30分钟，所述培养基包含Gamborg B5维生素、3％蔗糖、2mg/LBAP(6-苄基氨基嘌呤)和0.2mg/L NAA(萘乙酸)(例如，Duchefa,Netherland)。然后将细菌洗涤，并将叶片在黑暗中置于RM植物琼脂板上一天。将叶片转移到新的选择RM植物琼脂板上，其补充有300μg/ml的特美汀抗生素以杀死农杆菌和1.5μg/ml的磷酸肌醇(例如，Duchefa,Netherland)以选择转基因植物。图3A-B示出了用文库转化的烟草组织培养物的照片：转化后7天(图3A)和转化后40天(图3B)。6-8周后，小植株开始出现，并转移到包含相同选择RM植物琼脂但排除BAP的新容器中(见图3C)。生根后，将植物转移到温室的土壤中。

实例2

用于鉴定植物中的干旱抗性性状的方法

A.筛选干旱和/或盐分抗性植物/基因

将具有期望表达文库的拟南芥T1种子用于筛选。在第一阶段，确定特定种子文库的转化效率。根据制造商的说明，将1ml种子(约50,000粒种子)播种在用补充有Basta(例如，Bayer,Germany)的水灌溉的土壤上。播种后7天，对草丁膦抗性植物的数目进行计数并与对草丁膦易感性植物进行比较(图4)。如图4所示，较大的植物对草丁膦具有抗性，而较小的植物不存在转基因，因此是易感的并将死亡。种子库效率由抗性植物数目与总植物数目之比表示。

然后根据打算在具体实验中表现的最能代表文库大小的植物期望数目来播种文库。例如，如果表达文库由5X10⁴个基因组成，且转化效率为1％，则应播种>500万种子。在这种情况下，在约20m²的土壤中，将种植50,000个Basta抗性植物进行实验。

在种子播种时，应根据制造商的说明用补充有草丁膦和肥料(例如Shefer 5.3.8,ICL Israel)的水将土壤灌溉一次，并且土壤水含量达到100％容量。使植物在装有空调的温室中生长，并且不灌溉植物直到大多数植物因缺水而死亡。通过灌溉挽救存活的植物(初始草丁膦抗性植物的0.1％)，直到其产生种子，收集种子用于T2实验。在其生长过程中，通过从其一片叶的gDNA提取以及使用基因特异启动子(CaMV 35S、CBF3、ErdlO和Kinl)和终止子(NOS、HSP)的引物(参见表2)进行PCR来测试存活植物的转基因。对PCR产物进行测序，并将所得序列与序列数据库(如NCBI)进行比对，以进行DNA比较(即BLASTn)和氨基酸序列比较(即BLASTx)。

现在参考表2，其示出了用于本发明的引物和启动子序列的SEQ ID NO

表2：引物序列的SEQ ID NO

SEQ ID NO.	描述
		SEQ ID NO:321	用于poly-A mRNA转录的反向引物
SEQ ID NO:322	用于poly-A mRNA转录正向引物
		SEQ ID NO:323	用于cDNA末端的低封端扩增的正向引物
SEQ ID NO:324	用于扩增原核生物cDNA文库的正向引物(例如来自总RNA)
		SEQ ID NO:325	CaMV 35S启动子的正向引物
SEQ ID NO:326	CBF3启动子的正向引物
		SEQ ID NO:327	Erd10启动子的正向引物
SEQ ID NO:328	Kin1启动子的正向引物
		SEQ ID NO:329	NOS/HSP终止子的反向引物
SEQ ID NO:330	CBF3启动子
		SEQ ID NO:331	Erd10启动子
SEQ ID NO:332	Kin1启动子

B.后续世代(T2，T3)实验

将从干旱存活植物中收集的种子在包括重复和对照的另外的实验中再次进行测试，以测试其对干旱的抗性/耐受性(见图5)。

选择了多种基因作为干旱实验中的对照：

1)EGFP-水母绿色荧光蛋白，在多种使用的启动子和HSP终止子的控制下克隆(见图1A-D的载体图)，用作干旱的阴性对照，因为其未表现出与改良植物对干旱的抗性相关(Yang T-T等人,1996)。

2)mtlD-来自自大肠杆菌的甘露醇-1-磷酸脱氢酶，在多种使用的启动子和HSP终止子的控制下克隆(见图1A-D的载体图)，用作阳性对照，因为其表现出与改良植物对干旱和盐分的抗性相关(Hema R.等人,2014)。

3)HRD-来自拟南芥的HARDY基因，在多种使用的启动子和HSP终止子的控制下克隆(见图1A-D的载体图)，用作阳性对照，因为其表现出与改良植物对干旱和盐分的抗性相关(Karaba A等人,2007)

将在筛选实验中被鉴定为表达独特基因的植物(包括所有对照)播种在38x28 cm的托盘中，每个托盘中有16个塑料插入物(例如，Desch Plantpak,Netherland)，并如上所述用补充有肥料和草丁膦的土壤填充。在每个插入物中播种数粒种子，并且在10天后保留一株草丁膦抗性植物用于进一步的实验。每个实验包含代表了表达的独特基因的每种植物的20-40个重复，随机分布在温室桌上。土壤的灌溉与筛选试验类似。在种子播种时进行灌溉，只是当土壤完全干燥且重量达到低于灌溉前的土壤初始重量(水含量的5％-10％)时，再次灌溉植物以检查其恢复性能。

现在参考图6，其显示了筛选在上述条件下生长的对干旱具有抗性的转基因植物的照片结果。该图显示了携带干旱抗性基因10、20和30的转基因植物在严峻的干旱条件下存活，而其它没有干旱抗性赋予基因的转基因植物不能在胁迫条件下生存。值得注意的是，在该图所示的小区域(约15x25厘米)内，筛选了约300株植物，其中3株在干旱条件下存活。

当干旱条件开始发展时，将进行如表3所示的多种测量：

1)膨压观察，用1-10的量表测量，其中1为高膨压，而10为完全失去膨压(见图7)。

2)植物和盆的重量，以克为单位。

3)植物死亡观察，10＝死亡，1＝存活(见图8)。

4)植物状态观察，1-10的量表，其中1为良好状态，而10为差。

表3：干旱实验中的测量

现在参考图7，其显示了在停止灌溉后的数天中表达用作对照的基因的植物相对于土壤含水量的膨压损失(深灰色)。该图显示了表达不同基因(指示的)的拟南芥属植物的曲线，作为对干旱条件下生长的响应。深色线表示土壤水含量从停止水灌溉后第15天的40％到停止水灌溉后第22天的接近0％。阴性对照GFP植物膨压损失响应类似于表达HRD的植物膨压，而表达mtlD的植物膨压似乎很少受干旱的影响，直到停止水灌溉后20天。

在该图中证明，表达阳性对照基因mtlD和HRD的植物通过表现出显著降低的膨压损失效应而表现出提高的干旱抗性，而表达阴性对照GFP基因的转基因植物在暴露于相同的水含量损失时表现出提高的膨压损失效应。

现在参考图8，其显示与表达GFP的植物相比表达阳性对照的转基因植物的归一化死亡规模。可以看出，与表达阴性对照的GFP植物相比，表达干旱抗性阳性对照基因HRD和mtlD的植物显示出明显降低的死亡率。

现在参考图9，其图示了通过本发明的方法鉴定的多种干旱抗性基因的结果。

该图显示了在严重干旱条件下多种鉴定的基因的膨压(Tu)和死亡率(Dr)的平均结果(参见表4)。死亡和膨压损失的量表为1-10，其中10分别被认为干燥-褐色和死亡植物，或者膨压完全丧失。图中的结果表示播种后的第23天(1)、第28天(6)和第30天(8)。每个不同表达基因的每一个柱代表5个重复的平均值，每个重复具有4株植物。表达GFP的植物用作阴性对照，而表达HRD的用作阳性对照。可以看出，与表达阴性对照GFP基因的植物相比，通过本发明方法鉴定的所有测试基因均显示出显著降低的膨压损失(播种约23天后至少2倍)和降低了死亡率(播种30天后为9至2倍)。而且，与表达阳性对照HRD的植物相比，表达新发现的基因的植物(参见表4)显示出显著降低的死亡率。这些结果表明通过本发明的方法，鉴定了新的干旱抗性基因，其赋予植物改良的干旱耐受性。

用于评估植物在干旱条件下的性能的另一种方法是在干旱条件变得突出时，在生长阶段测量其叶面积。在播种植物后约10-14天，每2-3天拍摄植物图像，总共50mm²白色表面。对从干旱实验拍摄的照片进行图像分析，并计算叶面积。将表达在重克隆后鉴定为赋予干旱抗性的基因的多个植物品系的叶面积与阳性和阴性对照进行比较(见表5和图10)。

图10的图显示了转化植物品系的叶面积的图像分析。具有SEQ ID NO:16的经鉴定基因的两个独立的转化事件(图10A)和具有SEQ ID NO:25的经鉴定基因的两个独立的转化事件(图10B)以黑线示出在图10A和10B各自的顶部。将这些转基因植物与在图10A和10B各自的底部以浅灰色线表示的表达GFP的阴性对照植物和表达mtlD的阳性对照植物进行比较。干旱条件下改良的性能显示为在指示的测量时间点(TP)处相对于对照植物的百分比(图中箭头和百分比)。

从该图中可以看出，相对于表达阴性对照基因的植物，表达新鉴定的测试基因的植物的总叶面积增加了约10％至约82％(例如约45％)。

总而言之，本发明提供了新鉴定的基因，其被证明在植物中赋予对干旱条件的耐受性。

C.将所选基因重克隆并重转化到植物中

将来自B部分的选择的基因重克隆到如上所述的双元载体(即图1A-D)，并进行测序以确认其具有与来自T1和T2实验的原始基因相同的序列。用重克隆的基因转化植物，并收集种子。如B中一样重复实验，只是测试每个基因的3-5个具有不同无关转化事件的个体转基因植物。在实验中，每个单独的转基因植物/事件都要进行5-10次重复，因此，对于每个事件，每个基因测试20-40株植物，并且对于每个不同的基因，测试60-200株植物。

实例3

鉴定为在植物中改良干旱和/或盐分抗性的多核苷酸序列

筛选T1转基因种子的上述方法揭示了约1000个转基因作为候选多核苷酸序列，用于改良植物的干旱抗性。在这些候选物中，对T2种子的筛选揭示了约140个性能最佳的转基因，其可能改善植物中的干旱抗性或耐受性。对这些转基因序列进行进一步的验证测试。

现在参考表4，其给出了通过本发明的方法发现的新和独特的多核苷酸序列和由这些序列编码的多肽的实例。这些序列是从环境挑战生态位中纯化的宏转录组，

SEQ ID NO:1至SEQ ID NO:148代表通过本发明的方法发现作为改善植物干旱抗性的候选物的多核苷酸序列(表4)。

SEQ ID NO:149至SEQ ID NO:321代表由通过本发明的方法发现作为改善植物干旱抗性的候选物的相应多核苷酸序列编码的多肽序列(参见表4)。

应注意，以下DNA序列根据不同的起始密码子编码多于一个开放阅读框(ORF)(称为SEQ ID NO X.1p和X.2p等)：SEQ ID NO:9、SEQ ID NO:11、SEQ ID NO:20、SEQ ID NO:21、SEQ ID NO:28、SEQ ID NO:36、SEQ ID NO:43、SEQ ID NO:47、SEQ ID NO:51、SEQ ID NO:54、SEQ ID NO:55、SEQ ID NO:60、SEQ ID NO:75、SEQ ID NO:78、SEQ ID NO:80、SEQ IDNO:81、SEQ ID NO:85、SEQ ID NO:87、SEQ ID NO:97、SEQ ID NO:98、SEQ ID NO:105、SEQID NO:106、SEQ ID NO:107、SEQ ID NO:109、SEQ ID NO:111、SEQ ID NO:112、SEQ ID NO:120、SEQ ID NO:132、SEQ ID NO:134、SEQ ID NO:135、SEQ ID NO:140、SEQ ID NO:141。

表4：多核苷酸和多肽序列的SEQ ID No

现在参考表5，其示出了多种鉴定的基因在干旱耐受性实验中的表型结果。使植物在受控温室中的土壤中生长，并在上述条件下测试其干旱耐受性。在其生长过程中，进行测量和拍摄图像(参见表3)，并进行图像分析，将图像转换为每株植物的叶面积。结果显示为在干旱阶段用作阴性对照的表达GFP植物测量值的百分比。

表5：用T2拟南芥植物进行的干旱实验的结果

DR-干旱下的性能(叶面积)，以表达GFP的植物的百分比表示

SD-显示的值±标准偏差

如表5所示，与表达阴性对照基因(GFP)的植物相比，在干旱条件下，所有表达通过本发明方法鉴定的测试基因的植物显示叶面积增加了约15％至约90％。这些结果证明，本发明的方法提供赋予植物改良的干旱耐受性的新基因。

现在参考表6，其示出了用相关Seq.ID重克隆的T2拟南芥植物进行的干旱实验的结果。将不同Seq.ID重克隆并再转化到拟南芥植物中，产生多个独立的事件(表6中的E1-3表示)。使植物在受控温室中的土壤中生长，并在上述条件下测试其干旱耐受性。在它们的生长期间，拍摄图像并进行图像分析，将图像转换为每株植物的叶面积。结果示于表6中，作为在干旱阶段用作阴性对照的GFP表达植物的百分比。

表6：用相关Seq.ID重克隆的T2拟南芥植物进行的干旱实验的结果。

DR-干旱下的表现(叶面积)，以表达GFP的植物的百分比表示

RC E1-3-具有重克隆相关Seq.ID事件1-3的表现。

SD-显示的值±标准偏差

如表6所示，在经受干旱条件的拟南芥植物中，与表达阴性对照基因的植物相比，表达通过本发明的方法鉴定的重新克隆的基因的植物呈现出增加的叶面积。

现在参考表7，其显示了用T2烟草植物进行的干旱实验的结果。将通过本发明方法鉴定的不同基因重克隆并转化到烟草植物中，产生多个独立的事件(表7中的E1-3表示)。使植物在受控温室中的土壤中生长，并在上述条件下测试其干旱耐受性。在试验结束时，评估植物地上部分(shoots)鲜重、叶数、主枝长度和主枝重量。结果示于表7中，以作为阴性对照的野生型(WT)植物的百分比表示。

表7：用T2烟草植物进行的干旱实验的结果

FW-鲜重，以克为单位

LN-叶数

BFW-枝鲜重，以克为单位

BL-主枝长度，以cm为单位

SD-显示的值+/-标准偏差，为所测性状的百分比

E1-3-不同的独立事件

表7中显示的结果表明，与阴性对照植物相比，通过本发明鉴定的大多数基因赋予烟草植物对干旱条件的改善的耐受性，如通过测试的参数(例如鲜重、叶数、枝鲜重、枝长度)所示。

现在参考表8，其示出了与对照WT植物相比转基因烟草植物的盐分实验的结果。使表达通过本发明的方法鉴定的各种基因的不同烟草品系(参见表4)在土壤中发芽。发芽后7天；用含有400mM NaCl的肥料水灌溉植物。用盐灌溉后14天拍摄叶片图像，并分析不同独立事件的叶片面积。结果示于表8中，以相对于WT植物的叶面积差异百分比表示。

表8：烟草植物中盐分实验的结果

HST-高盐分耐受性，显示为与WT相比叶面积的差异％

SD-4个独立事件的显示的值+/-标准偏差

表8的结果清楚地表明，与WT对照植物相比，表达通过本发明鉴定的新盐分耐受性基因的植物显示出显著更高的叶面积。

现在参考表9，其示出了在表达具有指示的Seq.ID的新基因的拟南芥植物上进行的盐分实验。每盆每个事件十株植物在受控温室的土壤中生长。发芽后，将所有装有植物的盆通过浸入100mM NaCl中进行灌溉。表9的结果代表每个Seq.ID和野生型植物(WT)的4个不同事件的平均数据。

表9：在表达新的鉴定基因的拟南芥植物上进行的盐分实验的结果

FP-花和荚产量

1-没有花

2-很少花形成，短花梗

3-一些花形成，几乎没有荚

4-花和荚形成缺绿症-绿化和叶片损害

1-完全干燥的叶

2-干燥叶边缘

3-黄色

4-有些黄

5-绿色

±SD标准偏差

如表9所示，与经受相同盐分胁迫条件的WT对照植物相比，表达通过本发明的方法鉴定为赋予盐分耐受性的基因的植物显示出显著更高的花和荚产量，并且显著降低了缺绿症和对叶片的损害作用。

总而言之，上述实验结果清楚地表明，通过本发明的独特方法，可以鉴定出植物中非常有价值的胁迫耐受性(例如干旱、盐分)基因。新鉴定的基因赋予植物在各种重要参数如叶面积、膨压、气产量和品质、花和果实产量等方面提高的对预先选定胁迫的耐受性或抗性。这些结果表明本发明提供了鉴定胁迫耐受性植物基因的新的筛选方法，所述基因可以在期望和重要作物中表达，以使其在多种非生物和生物胁迫条件下生长并提高产量。

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序列表

<110> 植物ARC生物技术有限公司

<120> 用于改良植物性状的方法

<130> 1754-P-01-PCT

<160> 333

<170> PatentIn version 3.5

<210> 1

<211> 1019

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 1

ggactttctc attttcagaa ttattttcta tactctgaca agagcaagca ataccaaaca 60

tcttccacat cgaagcttta accattttgc ccttaacatt tgaacaagac gaaatggcct 120

tcttcccaca ctacaccact aatctgtcgc ctctgctcta cttgttggac gacgactatg 180

ctgtctaccg ctcaacttgt ccaaagtcca actaccacca caagcaacac cacagccgcc 240

gtcagccttc gccagttcgt tactttagtc cgaattttga tatgcgagag gggaatgact 300

cctactacct tgacggagag ctccctggtg tcaaccagaa tgatgtcgat attgaattct 360

ctgaccctca gacactggtg atcaagggtc gagtggagcg gaattacaac aatctcgacg 420

gcatgaacga ggaaaaccag caagatgaag aacaattctc tgaaactctc tctagcaagt 480

cgtaccaacc cactgtcgag gacgaggacg aggcgaacca ttcaccaccc gtggcgacac 540

caacctactc tgagaagtct gttactgaga aaactcagaa gcctgcgtac aaataccgaa 600

attctgaacg tgctattggc gaattccacc gagccttcaa tctccctaca agagtcgatc 660

aagatgcggt cagggctaca ttgaggaatg gaatcctctc gctggagctc ccgaaggagc 720

cggcaccgaa gatgaagaag attcggattg aatagaggat ttcgaataaa atttttgatt 780

tgatgagtag ttggtgttta ttgttatgtc taattatatg gggctatgtc atgattggga 840

aatgggacac cgcatttgtt tcctttttcc ccatttcttc agacgccatc tatattacat 900

gtatgttgca tgaactatgg tttttgctag gagcggttgc ttctgctctg cattttcatg 960

aactattttc tttttattaa attaataact agcatatcaa ttaatgatct gtcatatgg 1019

<210> 2

<211> 712

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 2

gatcatcaat caattaatca atctactcta ctttccaaaa cataactacc aaataaccag 60

aatgcagctc ctcagcaccc tcacccccct tgccctccta gtcaccgtcg cttccgccac 120

cggcaaagcc gtcaataatg ccgttggcaa cgccgtcgtc acaaaccact gtaaagaccc 180

aatctatctc tggtccgtcg gctcctccgt ctccccgaaa cacaccatcc cctccggctc 240

caactatacc gagcccttcc gccacgacga cgcatctggc ggcatcgcgc tgaagatcac 300

ccgtaacgac aacgggctgt atgacgggag tgcgcagtta gtttactcct acgctttgga 360

tggggaacag gtgtggtatg atttgtcgag tgtgtttggg gatgcgtttg caggggaggc 420

tgttgctgtg aagccggaga atgaggggtg tgggagtatt tgttggccta agggtaccac 480

gcctggtgga agccaggtta aggtctgtga tgcggagggg gatgttggat tggttgtttg 540

tgcgaagggg tgttaggggg tctgagtgaa ggttggtggt ggtaatgagc aattgggtat 600

gagaggggaa aggatatgtt aatcgtttat gttatttact tgatcaaaat atttgtattg 660

acgtcggttg ttttgttatt gttgttttaa atgcaaatgt atatgaactt tc 712

<210> 3

<211> 580

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 3

ggttcgtcaa cgcgacgatc cgcgggggtc caagctagga cgtggcagtt gtgacacaac 60

aagagcatgc tatggcaaat gccattcgcg agctcgccac taccctgtga actgtggcct 120

tactcctata cccgccagag tctgacttat tcctgtcact ggaatctggc ttactgctgg 180

tgctggagtc tggtcccagt attttagtat agtacaattg ctagctgaag ccataaggcg 240

tggattgttg gggtggcgca gggctgaagc caaatggcag cggtgttgct gctggttgag 300

caccgggcat agcgccagaa agtgcacccg cgaacattcc ctggtattgc atgaacggct 360

gtccaggaat cgcgccaggc attggcaagg cgttgttgcg ctttgcctcc gctgccagca 420

gcctctctgc ttccgtaccg tgtctttcgc ccttaccgtc cttcttgaat gcataatcaa 480

ccgtcagagg cttgttcatc aagtactggc cattcatagc cgtgattgcc tggtccgagc 540

tgtcaaagtc gttgtactgg atgaatccat atcctttcaa 580

<210> 4

<211> 580

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 4

ttgaaaggat atggattcat ccagtacaac gactttgaca gctcggacca ggcaatcacg 60

gctatgaatg gccagtactt gatgaacaag cctctgacgg ttgattatgc attcaagaag 120

gacggtaagg gcgaaagaca cggtacggaa gcagagaggc tgctggcagc ggaggcaaag 180

cgcaacaacg ccttgccaat gcctggcgcg attcctggac agccgttcat gcaataccag 240

ggaatgttcg cgggtgcact ttctggcgct atgcccggtg ctcaaccagc agcaacaccg 300

ctgccatttg gcttcagccc tgcgccaccc caacaatcca cgccttatgg cttcagctag 360

caattgtact atactaaaat actgggacca gactccagca ccagcagtaa gccagattcc 420

agtgacagga ataagtcaga ctctggcggg tataggagta aggccacagt tcacagggta 480

gtggcgagct cgcgaatggc atttgccata gcatgctctt gttgtgtcac aactgccacg 540

tcctagcttg gacccccgcg gatcgtcgcg ttgacgaacc 580

<210> 5

<211> 781

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 5

gaaaaaaact ttagaataca gtttaatcaa tcttcacagc tacaaggcta tatcatttga 60

tatagcatat caaagtggct ttgatttctg taaatttata tctaataata atagtgttta 120

tatcagctaa atacatattt ctatcctatc tatatatcac cgacagacca tatttgaaac 180

tgctgttgac actattattc atatgttcgg atttaatttt aatacgacaa aattgttaaa 240

aacaattctc gttgtttgtt atttgcaggc aacagtgtta gctgatcctt atacaagagt 300

atcttgggaa gcgtatatga atcatgtcaa tggatccgac gactatcgta ctcaagggga 360

tgataccaga gctacacgct ttccagagac taaacctcca aaacaaggaa aagatttcct 420

gtggtcgagt aaaccagtcc ccagttcaga tctatttctg gagttcttta tgtatgaggg 480

agaaccagat gaattcagca ggacgactga atcgtatcaa tcacttccga gcaacgcgtt 540

aactgctagg caaaatgccc ttacttgtca ggacatagag tcatgttcgt atcctccaca 600

ggtgaacaac tttcaagctt tattcgacga cctggggcca tcaacttgta atctcataaa 660

agacgaaact cgtgactgga tattgcagca gtggcccggg ttagctgtag gagccgttat 720

atcgtttgcg gtagccgttg cgggaagctc ctgtgatata ttatattaat cagctttggc 780

a 781

<210> 6

<211> 661

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 6

ggtcgagcta ctttcaaggt caagcaagat ggtccgttac gcacacaatg ctgagaaccc 60

agagaagacc gccaaggctc gtggtcagca cttgcgtacg cacttcaaga acacccgtga 120

agtcgctgct gctctgaccg gcttgaagct ttcaaaggct tacaagtacc tcggcgatgt 180

ccaagagcac aaggatgtca tcccattccg tcgcttcaac ggtggtgttg gcagagccgc 240

tcaggctaag aaccacggta cgacccaagg tcgttggcca gtcaagtcga ttggcttctt 300

gctcagactt ttgaagaacg ctgaggccaa cgctgacgcc aagtcactcg acacggaaga 360

cctcttgatc aagcacattg ttgtccaaca agctccaaaa acccgtcgtc gtacttaccg 420

tgctcacggt cgtatcaacc cttaccaagg acacccatgc cacattgaga tcactctggc 480

tgtcccagac gagcaagtcg ctcgcaacaa ggacgttgag gtgaaccaac caaagaagat 540

ccaaggcaac aagcgtcaag tcgctgctca acgtcgcttg acctctgcat aaactggcta 600

ctcggttgtg taccactcta tacaaattat tcagtaaaat gctatccatc ttggcttcga 660

a 661

<210> 7

<211> 713

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 7

agccacaacc acatcaatcc tccaccactt tcagctttcg acttcatcaa acaactcctt 60

ctaccactac tacctcaaca accttcatca aaatgactgg acgcggcaag ggcggcaagg 120

gtctcggaaa gggcggcgcc aagcgtcacc gcaagatctt gcgcgacaac atccagggca 180

tcaccaagcc cgccatccgc cgtctggcgc gtcgtggcgg tgtcaagcgt atctccgcca 240

tgatctacga ggagacccgc ggtgtcctca agaccttcct cgagggtgtc atccgcgacg 300

ccgtcaccta caccgagcac gccaagcgca agaccgtcac ctccctcgac gtcgtctacg 360

ccctcaagag gcaaggccgc accctctacg gtttcggtgg ttaagcagct cgctcttctc 420

tcttcgactg ctttgctttc ttcaaacaca ataacaatca cgacaacaac aacttcatca 480

gatatccacc cacaatgcga gagttgggct tgcgggtatg gcgcgaatgg gcaatgggct 540

atccgggttt tttcattttt ggggtttttt tctcttttcc tgtttcgatg ctgcgaggtg 600

agcacactgg gctgcggctc atgaggcttt gagtgtagaa taggctcaac atcatcaaag 660

aagcattcca cgagacgtgg cgctttcttc atcaaccaaa tgaatattgc agc 713

<210> 8

<211> 1021

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 8

ggacttgcga ccacacacat ctttatacct caaaatgtcg ctcgatgtcg gagatgtaga 60

cgcctggatc gacacgctat cgcagtgcaa gcagctatct gaatctgacg tgaagctcct 120

ctgcgacaag gccagagaaa ttcttataga ggagtccaac gtacagccag tcagatgccc 180

cgtcaccgtc tgcggcgata ttcacggtca attccacgac ttgattgagc tctttagaat 240

aggcggcaac tccccatcca ccaattacct cttcatgggc gattacgtag acagggggta 300

ctactcggtc gaaactgtca ccctcctcgt cgccttgaag ctccgctaca gggaaagaat 360

caccatcttg cgcggtaacc acgagtcgag acagatcacc caggtctacg gtttctacga 420

cgagtgcttg agaaagtatg gaaacgccaa cgtctggaag ttcttcaccg atctctttga 480

ctacctccca ctgacggcgc ttattgacaa tcaaatcttc tgtcttcacg gtggtttgtc 540

tccttccatc gacacgctcg accacatccg ctctatcgac cgtatccaag aggtgcctca 600

cgaaggtcct atgtgcgatc tcctctggtc cgatccagac gaccgctgcg gctggggcat 660

atcccctcgt ggtgccggtt acaccttcgg tcaggacatt tcagaggctt tcaaccactc 720

aaacggcttg acgctcgtag cccgtgctca ccaacttgtc atggaaggtt acaactggtc 780

ccaggacagg aatgtcgtca ctctcttctc tgcgccaaat tactgctaca gatgcggtaa 840

ccaagctgcg atcatggaga ttgacgagaa tctcaagtac actttcctcc aattcgatcc 900

agcaccaaga gctggcgaac cgatggtgtc tcgaagagtt ccggactact tcttataggc 960

tcttactcac tgtatttatg tttgcactgg gtattgttta cttgtacaat gtgtaactac 1020

g 1021

<210> 9

<211> 715

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 9

ggagccacaa ccacatcaat cctccaccac tttcagcttt cgacttcatc aaacaactcc 60

ttctaccact actacctcaa caaccttcat caaaatgact ggacgcggca agggcggcaa 120

gggtctcgga aagggcggcg ccaagcgtca ccgcaagatc ttgcgcgaca acatccaggg 180

catcaccaag cccgccatcc gccgtctggc gcgtcgtggc ggtgtcaagc gtatctccgc 240

catgatctac gaggagaccc gcggtgtcct caagaccttc ctcgagggtg tcatccgcga 300

cgccgtcacc tacaccgagc acgccaagcg caagaccgtc acctccctcg acgtcgtcta 360

cgccctcaag aggcaaggcc gcaccctcta cggtttcggt ggttaagcag ctcgctcttc 420

tctcttcgac tgctttgctt tcttcaaaca caataacaat cacgacaaca acaacttcat 480

cagatatcca cccacaatgc gagagttggg cttgcgggta tggcgcgaat gggcaatggg 540

ctatccgggt tttttcattt ttggggtttt tttctctttt cctgtttcga tgctgcgagg 600

tgagcacact gggctgcggc tcatgaggct ttgagtgtag aataggctca acatcatcaa 660

agaagcattc cacgagacgt ggcgctttct tcatcaacca aatgaatatt gcagc 715

<210> 10

<211> 503

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 10

aatcacccaa atgttctcta aactcatcgc catcgcctct cttgccctcg ctgccaacgc 60

tgcagtcatc gacccaagtg accacactgt ccaatacgaa gctgcaccag gaaaggttgt 120

gactgagcac tacgaggttc tcagccacgc cgaagcatcg cgcataatcg aagccaatcc 180

acacatcagc gactatcgct acagatgcaa ctaccaatgc aacgatagca gcggcaacta 240

catgagaaac ctgcagcagg gagttccaaa ccaagcatgc atcttctcta gctgctacga 300

ctgtgactgg aaattccaaa actgtagcta ctgtcgcttg tcgactggcc acaactaccg 360

tgatatcggt ggactcgaga gctggtgcta caacaacggc ggtactacag tgacgcacaa 420

ctgtggttat actgatggcg accaatgcta agagcggcct tgtaaagtaa aacttgtact 480

ctgaatttgc ctttatcttt ccc 503

<210> 11

<211> 580

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 11

atcatctcaa acccaattat cttgaacacc tagtttctca agaacatcct caaaatgcac 60

ttcaaatctc tctttattgc tggcgccctc ttcatggtcg gtgccagtgc cgttgattgt 120

gccactcctg agattcactg cgagactagt gatggcagcc cctggtacga cgatgccgtc 180

caagccactg aatactggaa agaaatccag gacgccggca aagacagctg cggtgatgct 240

ggttgcgcac agccccatgg ctctggatgc cacagcgacg gtggtagcta tggtaccgcc 300

gagatcgttc tctgccagga tgactcgtcc tcttcaactc cccaatgtgc cgactgccgg 360

tgtgtctaca gctacctgaa gcctcttctc gaccaatgca agggtgccaa caacaagatt 420

ggtggatatg ctcatgttga catgggaggc aactacatca actatgaatt tgttaagaaa 480

tgagcggatc tcacgtgtgt gagaccatca tatagggttt tgaagtctgt ttcctttgta 540

tttaacgtcg aaagacaatt atgagccagg tttatactcc 580

<210> 12

<211> 607

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 12

cgcgccgggg gaaattggac catgatagac gacctcacca ctggctcaga ggacagcttc 60

tccaacagct ggatatcgtg gttcttatct accaaaggga acgagtactt ctgtgaggtg 120

gatgaggagt acatactgga cagattcaac ctcactggcc tcaacaacga cgtgcagaac 180

tactcgcagg cgctggagct catcacagac agcctcgacg acgaggacct cgatgatgag 240

cagagagacg ctatcgagaa cagtgccagg tatctctatg gcttgatcca cgccagatac 300

atcattacct cccgcggact ggcaaagatg ctcttcttgg tgtacccgca gcagctgccg 360

tcaaagacga cgaactcagt gccgagcacg aagccggcaa cttcagcaga cgcagcggtc 420

ggggtggaca ggtacctgcc caagatattc gggttcccgg tgcacgagat gtccaagcac 480

gcgaggtggc aggaggcgca gagggatctg cagatttcga ggctgcagca aagtgcgagt 540

gacccgtcgt acgtgtagag cgttcaaaca tgtattacta ttggtataat aatttaactt 600

tactgcc 607

<210> 13

<211> 1406

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 13

aagttacccg gcttattagt cctagattcc gagatgtcgc tcactcccga acaaaccgaa 60

atcatcaagg ccaccgtgcc tgtcgttaaa gaacatggca agaccatcac caccgttttc 120

tacaagaaca tgctcgaagc gcatcctgag ctgaacgcca ttttcaacac taccaatcag 180

gtcaatggtc accagcccaa cgcactcgcc ggagccctct tcgcctacgc ctccaacatt 240

gacaaccttg gcgccttggg ccctgccgtc gaactcatct gcaacaagca tgcttcgctg 300

tatatccaac ctgagcacta cggcatcgtc ggcaagttcc ttctcgaagc gatgggacag 360

gttttgggtg acgccttgac tccgcagatc ctcgacgcct gggcagctgc ctactggcag 420

ctcgccaacc tctttattgg tcgcgaaagt gctatctaca agcagagtga gggatggaca 480

cagtggcgcg agttccgggt tgcacagaag gtccctgagt ccgcggagat cacatcgttc 540

tacctcaagc ctgtcgacga gaagcctttg ccccgcttcc gccccggaca gtacatttcc 600

gtccaagtgc acgttcctca gcttgaatgc ccccaagctc gccaatactc cctcagcgac 660

aagccccgcg acgattacta ccgcatcagc gtgaagaagg agacgggtct caacacagca 720

aagccggagg ccaaggtcaa cccgggttac gtctcgaata ttctgcacga gaacgtcaac 780

gagggcgacg tgatcaaggt gtcgcaccct tgcggcgatt tcttcttgac cgagcaggaa 840

ccgtcgcacc ctgtcgtcct catcgcagcc ggtgtgggtc tgacgccact tacctcgatg 900

ctcaacacat tggactccac ccccgcggac tctcagcgca agattcactt catccacggt 960

gcgcgcacca cttccgtccg cgctttcaag gaccagatta agtctcgcgc tgagcgactc 1020

ccgaatctcc aggccacctt cttcaccagc tccccgtcgg cagatgaaaa gcaaggcgtc 1080

gactatgacg tccagggccg tatcgatgtg tccaagatgg atgccagcaa ggatcttttc 1140

ctcgacaatg cgcagaccga gttctacatt tgtggtccca cttccttcat gaatgatatc 1200

gcgaacagct tgaaagctcg gggggctacc tcggagcgta tccacatgga attgttcggc 1260

actggcggcg tgcctgttta gatgatggct cagttagccg tgattgggtt ttatttcttt 1320

acgacgatat gactcaggtt tctaagttag tatacataat catgataaat tcttatatag 1380

atatatcaat aatacatctc ctctcg 1406

<210> 14

<211> 750

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 14

gggtctcttc catttgaatt tttcaaccca cagcatggcc ttcatgaatc tcccatggcc 60

cactgaatgc ctgcatgccg ctctcaagaa cggatcctta cctttctggg gatttgtaat 120

ctatcgaacg acctacaccg ctcagtcaga tgccgcctgg ccgcagatta tcgagcttat 180

tgcctcctat atgaaagcct tactctacca cgagtataac gacaagaaaa aagatggaga 240

tgagcctaca gtctacgacg aaatctgggc aaggcatcag ttgacgatta tggatgatag 300

acaattcaac ggagcgtctg tgtttgatat ccaacttcac ttcgaaaagt gggttgaggc 360

gcagggaaag cgagatgaat ctactatgta tcgcatgtgt atggtcattg atgatgaatc 420

aatccagacg ttattggagg cgccacccgg ggaaaatagg aaactcggac gacgtatagg 480

gggccctgta cgctttgtca aagtcgtgga ggctttcccc gagctagaca gccttgacga 540

attccaggga tggatgaaat gtgagatcaa cgcgttatgg ccgctgtgga agatgatgtc 600

tgacggagat gaaatgagga tgtcatatga tgagatgaag gggaatggaa agcaggtcta 660

tggcgcaatt taatcggttt ttcttcatgt tatcctgatg gaaaaaatgg cagaacatat 720

gtctgtacat gcagaaaata aggtgattgg 750

<210> 15

<211> 811

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 15

gacaccacct ctttttcgac aaccacaccc cgtttcgcag gaagtccatt tccagcagtc 60

aaaatggccc gtcgtcccgc gagatgttac cgctactgca agaacaagcc ttaccctaag 120

tcccggttca accgtggtgt tcccgacccc aagatccgta tcttcgactt gggtcgtaag 180

aaggcttccg tggacgactt ccccctgtgc gtccacatgg tctccaacga atacgaacag 240

ctttcctccg aagctctcga agctgcccgt atctgtgcca acaagtacct cgtcaagatc 300

gccggtaagg aaggtttcca cctgcgtgtc cgcgcccacc ccttccacgt cgtccgtatc 360

aacaagatgt tgtcgtgcgc tggtgccgat cgtctccaga ccggtatgcg tggtgccttc 420

ggtaagccca acggtgttgt cgcccgtgtg aacatcggcc agatcctcct gtccatccgc 480

acccgtgact ccaaccgcgc cgccgccgtt gaggccatgc gccgctccac ctacaagttc 540

cctggtcgcc aaaagatcat tatctccaag aactggggct tcacccccgt ccgtcgtgag 600

gagtacgtca agctccgcca ggagggcaag ctcaagcagg acggtgccta cgtccagttc 660

ctgcgtggcc acggtttggt cgaggagaac atgaagcgct tcccccaggc ctacgagggc 720

gttgctcagt agattgggat gaattaggtg gttttatgtg ctggtcgtat ttatcgtttt 780

tactagggcc aaatgagaac aaaaaaaggc t 811

<210> 16

<211> 923

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 16

gtctttagtt ggccattgaa cactgacaga tttgcattgc ttatatttgc atctacctca 60

catctactca actcctcctc tccgtttgtc atgtccttct accagtctcg tccagacact 120

atcaagggtc ctgatccttt gaccgacaat tggacttatg atagtgccat tgatctcttc 180

tcttggaatc ccatgatgcc cgatcctttt acctttgacc tgcccgacga tcttatgaaa 240

tttgaatcta aggatatgtc tgctggcatg gtcgctcctt cggacattag tggttttgcc 300

attggtaacc atttgggcga ggatgctgcc tcgatatctg atcccgagag tgatgaccac 360

ccatggtccc cctccgctca tgctgccttc ccggagctct ctcccatcac atcgacagag 420

caagtccatc aagaaactgc tcgatactca actacccccg atgccacctc acctcaagaa 480

caaccctcct caccaccaac acgatctact cgccgccgat catccgctga cggtcccgtt 540

cgcaacgctg ccaaacgagc agcccacaac gtcattgaaa agcgctacag aacaaacatg 600

aatgccaaat tcgtggcact cgagaaagca atgaatggcg gtaatggcgt gcaaacatca 660

tcaagaggcg gagggtccgc gtcgcttaag aaatccgaaa tcctctctaa tgctattgcc 720

tacatgcatg gactgcaaga ggaaaatcgc tatttacaaa aggagcttgc tatcgttaaa 780

cagaatcttg taccggcagg gatatggcga ggggctccta gttgtaaacg ggagacgagt 840

tatcgttaac ttgttgattt ccctgtggtt gtttagattt tttttacgat gttacgtgta 900

taataatact ctcccctcgg gtc 923

<210> 17

<211> 1046

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 17

ggacaagccc atcttcaatt cgagacagtc gccatgggtc gcgttatccg caaccagagg 60

aagggccgtg gctccatttt cacggctcac acccgtctga acaaggctcc cgcccagttc 120

cgtaccctcg acttcgctga gcgtcacgga tacacccgtg gtgttgtcaa ggagatcatc 180

cacgatgccg gccgtggtgc tcccctcgcc aaggtccagt tccgccaccc ctacaagttc 240

aagatggtga ccgagacctt catcgccaac gagggcatgt acaccggtca gttcatctac 300

gccggtaaga acgctcagct caccgtcggc aacgttctgc ccctcgcctc catgcccgag 360

ggtaccgtca tctccaacgt tgaggagaag tccggtgacc gtggtgcgct tggccgtacc 420

tccggtaact acgttaccgt cattggccac aaccccgagg acggcaagac ccgtgtcaag 480

cttccctccg gtgccaaaaa ggtcatcaag aacaccgccc gtggtatggt tggtatcgtc 540

gccggtggtg gtcgtaccga caagcccctg ctcaaggctt cccgcgccaa gcacaagttc 600

gccgtcaagc gcaactcttg gcccaagact cgtggtgttg ccatgaaccc cgttgatcac 660

cctcacggtg gtggtaacca ccagcatatc ggtaaggcct ctaccatctc ccgctacgcc 720

gcccagggtc aaaaggccgg tctcattgct gcccggagaa ccggtctgct ccgtggtacc 780

cagaagacca aggattaagc gtgatattac gtggagtttt ctttgtgacg ggttgaaaat 840

ggacttctgc tatgagacat atgtacttag gcgagtgcgg ataagcgtcc catgcgccct 900

tagcgaatta aggttgtggt caccatcctt ttctttttat taaatcaaaa aagggtgatg 960

gaatggggtc cgaggctggc ctcaagtcaa ggcagaacgg aaaagtcaaa aatgcccctt 1020

ggggttttgg aaatgataca cctttg 1046

<210> 18

<211> 780

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 18

ggcgcagagg cctattactc cccagtatca tcgctaatag gcatgtccac gggtctaagg 60

ttcagcactt tgccagctgc ttccaatcca cagtcgtcgt cattgatacc cagccctagt 120

gctcccatat caacttttcc atatacttta acactcacgc taactccctt gacgggatcc 180

ctctcaacct catactcatt acgcgcctct cccaatctgt catttagctc tcggttcggt 240

ttcaatgttt acagttggga aagcgagatg gtagcgggat ttgaactatg gcgacaatcg 300

aaaaagccca agttggccgc gggaagcgat ggcgacgatc ttgaatgggc ccgcaggaag 360

gtccgtgtct gggatccctc agcttttccc ctggcacccc ctgaacctga aatcccacaa 420

ccaaaccatg aagatgagtc tcaagagtca gtacttaagc tacgagtcga ccaatcctgg 480

aatgttcgtc ttctctggga aggtcgggtg aaggagcttt tggtcagcgc tggtgtcggg 540

ctcggcccga gttccttctc accatcgtca tatgcaaatc ccccgggtac agccggggct 600

caaggcagcg gtgggggctc accggcctca tactggaggg gcgtgggggg tttcggtatc 660

atattcttca tgagggattt cttcggatct atgtacttga atgagcactg tctagatgta 720

tatagtttat cagattttat gagacaatag acaccatgaa tctgcgttat tgcgagacgg 780

<210> 19

<211> 896

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 19

ggccctggcg tgctttctgg ctttcaacct cccgacctcc ctctaattac ctcaattgaa 60

ctcgatttag acgtggtgct gccacctccc gcctgccgca caatgtttct tcgcaccgtt 120

tctcgcgctg tccctcgcag caccgcggcc atccgtgctg caccgactgc ctctgtgaac 180

gccctgcaga cccgcgctgc ctcggaccat gctatcccca accctaccct cgccaacatt 240

gagaagcgct gggaggtcat gccccctcag gagcaggccg agctctggat gcagctccgt 300

gaccgcatga aggttgactg gcaccagatg accctgcagg agaagaaggc cgcttactac 360

attgccttcg gcgcccacgg cccccgcgcc cagcccccca agggtgaggg catgcgcgtg 420

ttcgccaagg tgctccagct cactgccgcc tccgttgctg tcttctacgc catccacgcc 480

ttcgccggca agcagcccgc caccatgtcc aaggagtggc aggaggcctc caacgaatat 540

gccctgaaag agaagatcaa ccccatccac ggcatcagca aagagggtta cgaaggcaag 600

ggcttcgtcc agagcccccc tgccgagaag tcataggtgt accagttgcc cgaccgggaa 660

tgagttgata tctacgccgg acggacggcg gcacccatcg cacgatctat atgtcgatct 720

tattacaagc tactctttcc atagccatgt tcgacatgtc tttgtgtcgg aggatgggcc 780

tccgcccgtg cgcgcggccg tcgattgttc cattctatct tttttggcaa gcattggaaa 840

atgcgtgtat cccgtactgt gctataatca atgtatctct tttgtagcca tagagc 896

<210> 20

<211> 641

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 20

gtggcgcgcc gggggggcat ctacctcgac ggcaacaacg acctggtcac tatgaagggt 60

aactacatct accacaccag cggccgctct cctaaggttc agggtaacac cttgctgcac 120

gctgtcaaca actactggca cgacaactcc ggccacgcct tcgagatcgg tgagggtggt 180

tacgttctgg ccgagggtaa cgtcttccag gatgttacta cccccgttga ggaccccgtt 240

gacggccagc tcttcacttc ccctgacccc agcaccaacg ctcagtgctc gtcatacctt 300

ggccgggcct gcgaaatcaa cggcttcggt aactctggta ccttcaacca ggctgacact 360

agcctgctgt ctaaatttaa gggtcagaac attgcttctg ctgatgctta ctctaaggtt 420

gcctcgagcg ttgccagcaa cgccggtcag ggacacctgt aaaatggaaa gaggaggttc 480

agagcttaat ttgctcatgt cggacgacat agccctagcg gcttgctggt gaatttggca 540

taatagcgtt tctcttctca tacctacttt attactccgt ttggatcctt attaggtaaa 600

tattagccca ttgtatggtt caattcgatt gactttgagg c 641

<210> 21

<211> 591

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 21

gtggcgcgcc gggattctca tcatcagata aaatcaagat taatcttact ggacatcaca 60

acgatccaac acaaagttcc ttcatacttc aaacaaatct ctacaattga atcaaaatgc 120

catccaaaac cgaagcagcc cgtctacaaa acgacttcgg cgcagactac tgggttagaa 180

atacccaaga acgccgccac tcaaccgctg gccgcggact attcgccggt ctccaggatg 240

tcaagcacta taacgtcgac catggctggg cccgtcgcaa gtctagcgat aaccccggac 300

tccttgcttc tttcttcagt cgattcaccg ggggatcata ccatccgccc tcggaataga 360

attccttttc ttaatgtgcg atattgggag gagtgtgatt tgaattggga ataagggaaa 420

agagtgcttg gaatatttga gtctcagact taactcgagt caagtttcat ttatgagtat 480

actgaggttt ttgtgttagt agcttggagt ttgggtggtt tattagtatt acctattgca 540

ttaccatgtt tatacatcgt gaatcatcga atgaatacca tgtcttcaat t 591

<210> 22

<211> 639

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 22

gtggtatcaa cgcagagtga cgagcccacc atccccggag gcgccgctgt caccatccac 60

tcccgtaacg agaagaaggc ccgtaaggcc attggcaagc tcggtctcaa gcacgtcccc 120

ggcatcaccc gtgttactct ccgccgtcct aagaacatcc ttttcgttgt taaccagccc 180

gatgtctaca agtcgccttc cagcaacacc tggatcatct tcggtgaggc caagatcgag 240

gacctgaact cccaggccca ggcttccgct gctcagcagc ttgccgccgc cgaggctgcc 300

gccggaggtg agcacgctgg tcacgaccac gagcacgaca tcctcggcaa gggcaaggcc 360

cccgagaccg agggcaagaa ggaagaagag gaggacgacg gcgaggaggt tgacgaggcc 420

ggcctcgagg ccaaggacat cgaccttgtc atggcccagg ccaacgtctc ccgcaagaag 480

gccgtcaagg ccctccggga gaacgacaat gatatcgtga actcgatcat ggctctcagc 540

atatgatttg gctgcctgcc ggcaggatga atgagtgagc tttgggcgcg aggtcacgtt 600

gatatccctg ttctgggccc tctcccttaa gtgtatagc 639

<210> 23

<211> 832

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 23

gctcccaacg tcaacacccc ctccgccttc ccctcgaccg ttactcctgc ccgtccgatt 60

acaacaagga gaatgttcct tcagcgtacg gtatctaccc tcgcgaggcg cacccccgtg 120

cggggccttg ctgccgcgcg cccgttttct tcgtccgtta gccgattcaa caagtacgag 180

gttaaggagg ccaagctccg ttctcttgac gagatccaaa ctgaagaaga cctcatcccc 240

cctggtgcta agcccggtac cgtccctagc gatatcgaac acgccactgg tctcgagcgt 300

ctcgaactcg tcggtaaaat gcagggaatt gacatcttcg acttgaggcc tctggatgct 360

tcccgcaagg gaaccctcga aaaccccatt gttgtcaacg gtgctggtga cgagcagtac 420

gctggttgca ctggttaccc cgtcgactct caccaggtta actggttgac tgtctctcgt 480

gagcgcccca tcgagcgctg caacgaatgc ggtaacgttg tcaagctgaa ctatgtcgga 540

cctgaggagg accctcacgc tcacgaccac ggccacggcc accaccctgc ccccgaggag 600

cccaagacct tcgccgacta cgtcaagccc gagtactggt accggtaaat accccagcag 660

tacgacgcga gagttttcaa aaaagagaat aagaaacaag caaagggacg gatcaagacg 720

ggctagtgcg ggaatgtcaa acgcaacata tttaagcatt gggtctacta tatacgggtt 780

cattcgtcca ttgattcctc ggtctagtgt tttcttgaac gtctttagct gg 832

<210> 24

<211> 832

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 24

ccagctaaag acgttcaaga aaacactaga ccgaggaatc aatggacgaa tgaacccgta 60

tatagtagac ccaatgctta aatatgttgc gtttgacatt cccgcactag cccgtcttga 120

tccgtccctt tgcttgtttc ttattctctt ttttgaaaac tctcgcgtcg tactgctggg 180

gtatttaccg gtaccagtac tcgggcttga cgtagtcggc gaaggtcttg ggctcctcgg 240

gggcagggtg gtggccgtgg ccgtggtcgt gagcgtgagg gtcctcctca ggtccgacat 300

agttcagctt gacaacgtta ccgcattcgt tgcagcgctc gatggggcgc tcacgagaga 360

cagtcaacca gttaacctgg tgagagtcga cggggtaacc agtgcaacca gcgtactgct 420

cgtcaccagc accgttgaca acaatggggt tttcgagggt tcccttgcgg gaagcatcca 480

gaggcctcaa gtcgaagatg tcaattccct gcattttacc gacgagttcg agacgctcga 540

gaccagtggc gtgttcgata tcgctaggga cggtaccggg cttagcacca ggggggatga 600

ggtcttcttc agtttggatc tcgtcaagag aacggagctt ggcctcctta acctcgtact 660

tgttgaatcg gctaacggac gaagaaaacg ggcgcgcggc agcaaggccc cgcacggggg 720

tgcgcctcgc gagggtagat accgtacgct gaaggaacat tctccttgtt gtaatcggac 780

gggcaggagt aacggtcgag gggaaggcgg agggggtgtt gacgttggga gc 832

<210> 25

<211> 1263

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 25

acccaaaccc tgcgaagcaa aacgcgttac tcgatttggt catttcctcc aaagcatcat 60

tcctctttgg gcactgcctg tttcgtccat taatcaactg tgcttgaatc tctaactatc 120

ttttgatata ccctctttat ctctctcccc tttaatcttt ttttctctct ctctcttttc 180

ctttcttttt cggttactca ctatcatggc cgacatcact gccgtcggtg aggagaaccc 240

ttctcctacc caggatgagc tgcagcaggc cgcggccggt aacggcgctc ctgataaccg 300

cactcccaag cgtcgcatga gtgacgatga agaggacgag gagaagcagg gtcgcgagcg 360

cagaaagatt gagatcaagt tcattcagga taagtcgcgt cgccacatca ccttctccaa 420

gcggaaggcg ggtatcatga agaaggcata cgaattgtcc gtcctcacag gcacccaggt 480

gctgttgctg gtcgtgtccg agaccggcct ggtctatacc tttaccaccc ctaagctcca 540

accattggtc accaaggcgg agggcaagaa cctgattcag gcttgcctca acgcccccga 600

ccctaccacc agcgagaatg gcgtcgatgc ccccgaggtc ccagcggaga cccccgagga 660

tgtcaaccac gccaacgtca acgctgccgc agcccagcag accaacatcc ctcgtcccac 720

cggaatgcat cccggctaca tgaccaacga acaacagcag cagatggcct actaccaaaa 780

ccacctccag cagcaacagc aggccggtgg gcagtaccct ggcatgtctg tcggtggtcg 840

catgcctacg cagcaccagc ctaccgcata atcttattta ctcttatcta cgctcccacg 900

cacctcctct ttctgatttc cctgcatatg gtcttgtttt tagtagctga ggagtccaga 960

gttcagttgt ttttgccttc tttccgcatc taccctttat tttcccctct ttcgttatta 1020

tctctctccc ctgacatttg atacccgaca atcctgttgt tcaatccatc gtcgcatgaa 1080

aacgggtcct ataaatataa tgcatccccc tgtttacttt cgactgcgaa cgagagcatg 1140

caaatctgaa gaacagcatg gtcaattgtc tcagtaacct cgttaaggcg ccgatgagtt 1200

tggcgtttac atactctgct ttggaacgtg tgatgccttt ttaccgttca atgaaagcga 1260

ctc 1263

<210> 26

<211> 1066

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 26

aggctttgat ggcctcttca aaggggaaca ttgttcactc gaccagagtt gagtagagcc 60

aagaccattc cataatctcc aggggcatat cgccatgccg tcttgtcaag acgatctcct 120

tctcacacat attgatgata ggtaccgtta gattggactc ggttgaaccg agtccaattt 180

ggaactacga ctcctcccgg gcctgatttt gcaacgagga ttccactccc gtacactaca 240

gaactatatc gacaccgtcg ctgagattca atgtattctt tcattcgact ggcttcttcc 300

tccgggtgag acgagctgat atttgggaga aatattggac atcccagata atctcgcgca 360

aagtctagtt tggtctggtt gatatcggaa atgatcactt gtttggctcc aaacgctgtg 420

gccgtcgctg cacagaacag accgatagtc cccgacgctt gtaccaggac agtatgaccg 480

ggagtgatac ctgccacccg agctccatga attgcaacac tcaacggctc gaccaaaaca 540

gtttcttcaa gtgagaaatt ttcgggaatc ggatacacca aatcctcggg tgcgcggaat 600

agatgggtca gagtcccatg gttattgggg ggaggatccg cggcaaagct catctctgga 660

aaaatatcat atctccctgc tttacattgt ttacatcgtc gacgagagaa actagctcga 720

tggcaaagcg gtcgccagga atcacttttg tcactgccgg tccaattgag tgcacgatac 780

ctgatgcctc atgacccatg accagtggtt gctcgtcaga gaccattcga agtaccccgc 840

cgtgtttcca gaaatgggcc tatggaattg gaaattagca aaagagaccc tggtgaaagg 900

aagagggatt tgtggggact catatcgctc ccatacacac ccacatacgc gatgcgaact 960

aatatatcat agggatcact gagggtaggg acatcgcggt actcaagtcg agctttccca 1020

ggcccgtaga gtaggcagga caaattattc tgaaattatg atcaac 1066

<210> 27

<211> 1066

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 27

gttgatcata atttcagaat aatttgtcct gcctactcta cgggcctggg aaagctcgac 60

ttgagtaccg cgatgtccct accctcagtg atccctatga tatattagtt cgcatcgcgt 120

atgtgggtgt gtatgggagc gatatgagtc cccacaaatc cctcttcctt tcaccagggt 180

ctcttttgct aatttccaat tccataggcc catttctgga aacacggcgg ggtacttcga 240

atggtctctg acgagcaacc actggtcatg ggtcatgagg catcaggtat cgtgcactca 300

attggaccgg cagtgacaaa agtgattcct ggcgaccgct ttgccatcga gctagtttct 360

ctcgtcgacg atgtaaacaa tgtaaagcag ggagatatga tatttttcca gagatgagct 420

ttgccgcgga tcctcccccc aataaccatg ggactctgac ccatctattc cgcgcacccg 480

aggatttggt gtatccgatt cccgaaaatt tctcacttga agaaactgtt ttggtcgagc 540

cgttgagtgt tgcaattcat ggagctcggg tggcaggtat cactcccggt catactgtcc 600

tggtacaagc gtcggggact atcggtctgt tctgtgcagc gacggccaca gcgtttggag 660

ccaaacaagt gatcatttcc gatatcaacc agaccaaact agactttgcg cgagattatc 720

tgggatgtcc aatatttctc ccaaatatca gctcgtctca cccggaggaa gaagccagtc 780

gaatgaaaga atacattgaa tctcagcgac ggtgtcgata tagttctgta gtgtacggga 840

gtggaatcct cgttgcaaaa tcaggcccgg gaggagtcgt agttccaaat tggactcggt 900

tcaaccgagt ccaatctaac ggtacctatc atcaatatgt gtgagaagga gatcgtcttg 960

acaagacggc atggcgatat gcccctggag attatggaat ggtcttggct ctactcaact 1020

ctggtcgagt gaacaatgtt cccctttgaa gaggccatca aagcct 1066

<210> 28

<211> 564

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 28

agactcaacg ataattcaat acccagttgc aacactattt gttttctaaa catatcctca 60

agaccaattc taccttaatc tccccaacac aactcaattg aaaacataca ccatgcctcg 120

cggagccgaa tacgccaacg gtcctctcca gagcgacaat gccatcgaag ctggcgaaaa 180

taaggcccac ggaacctccg gtaacaccgg cctcaaccgc gtcaacaagg tcgccgaatt 240

ccccgaaggc gccagaggaa ccggtaccgc tgctaacccg ctcagtggcc agggtagcgc 300

cggccatcag gatggaaagg gtggccatga cccgaagacc cttggagaga acaagggact 360

gggtactcaa tgatcttatg attcagaaga catgagttat ttgcatgagc tgggctcgct 420

gcgattctgt gggattctgt gatttgtaat atgatttgca tgggtcaggt cagacttaat 480

taagcatgcg ctattgtttc cgttatgctt atgatatgga tgggtccatg gttggagttg 540

ataatctaat atggaattga agtg 564

<210> 29

<211> 39

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 29

attccatcta aagctaaata ttggcctgag accgatagc 39

<210> 30

<211> 577

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 30

gagactatga cgacgatcac agagttcccg ccattctaca cgcagcagcc gaatgcgagc 60

gcgctgacgc agcagctggg gctgtggcag aagcatatac tgagcacgtg caagcagcgg 120

cggcagttca agctgagcgt gagtgatgat atctgggcca acgagaggat aaagcgagct 180

gcttctcgtg aatttatttc tgtgattatc tcctcgctgg tgacagaagg gctagcgagc 240

tatacagacg ccaccaagga ggctgtgtgg gtgtactggc ggagtctatc tgattgggcg 300

caggcggcgt acgcgtacgc ggaaagcaca gcgcagctga acacgccgtt gacgtactat 360

gagctagtac aaggggagta cagccatcta tctgagctgc atgagatgcc agtagagctg 420

ctcaagcttg ctgtgtcgct gctggtgaag cagaacaaag cggtgataat caaaacgagt 480

caaggggaag gtgtcaaatt cgtctagtat agaataactt aggttacatt ggaatctggt 540

aatcaattcc cttgtcattc agcttctgct gctttcc 577

<210> 31

<211> 606

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 31

gggaacaaac tctcattcta actaaatact ttttactctc tgctccccta tcgcattctt 60

tttaggacat tcagaaggtg atcgcttgac caaatgtcta tcccaaaagc ggcagctcat 120

accgacaagg cgcctcagcc tttcaaggac ctctattcgc aagcagttat tgctggtggc 180

gtggtctatt gctctggaat tgttgccatt gaccctgaaa ccggtagcct gattgaagga 240

gatgtcaagg ctcatacgga acgaatttta caaagccttt ctagtactct acaggccgcc 300

ggtaccagtc ttgatcgagc tgtaaagatc aatgtttacc tagcaaacat ggaagacttc 360

acatccatga actcagttta cgaaaagtat tttgtggatg gagtgaaacc ctgcagaacc 420

tgtgtggctg ttaagtctct accttttggc actgatgttg agatggaatg cattgcagta 480

ctgtaaatgt ttagttttat gcgcaactga gaaagacgga aggatcatcc tattactttt 540

tcgaatgtgc tctttggatt tctctgttgg atacacaaca atgccacaca ttgggtacaa 600

ccagat 606

<210> 32

<211> 411

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 32

ggaaccaatt acatccatca accaaaccaa caaaatgctg agatctcaat tcggtgtaat 60

ttcaaacgca gcaaagacag ccgcattcct caagcctgtt caaaccagat tgtacgctag 120

tggcgctctc tcgaagggcg acatccaaac tcgcattttt gatgtcctca agtcgtttga 180

taaggtgaag gctgataacc tcactgaatc ggcttctttc accaacgacc tcggcttgga 240

tagcttggac gccgttgaag tcgttatggc cattgaggag gagtttgcca tcgaaattcc 300

agacgctgaa gctgacgcaa tccaaaacgt gaaccaggct atcgaataca tcgccaaaac 360

ccctgaagca cactaaacac gctaaataat tttatcaatt catttcaaac g 411

<210> 33

<211> 619

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 33

gtggtatcaa cgcagagtgg cgcgccggga acgaagaaag tttcttcgac ctacctatca 60

aagatatgta aggagcaaca taaatcaata ttcttttatc tgattgacct tcactctagt 120

gcctcgctac gatcagttac aaccttgccc gcgcgaatgg aacgtatacg actttccgaa 180

caggccgcga cgatttgcaa ccaaattcgt gaaatgatac cagagactgc cactttgccc 240

aatcaacctg gcaaggatca agctgaactc atgcatgaag atgaaaacgg gaataagata 300

tacggcggga aacttttaac ggagagagct gctcgactga aagagcacat gaagattgac 360

caagtgagtg ccagatttat ctcacagtac tttactaatg gcattcagga ctggacagag 420

cgcttggtat attggacaaa gccgacgaaa ctattgaacc aacggaaaca aggatatatc 480

ataccgttat ctaaagacat cgttctacaa cctgggggac ctttagaagc aaataacggc 540

tttcgggtca caaacgagcg gattctgagt tcaggagctg cccttttcat tatgccccaa 600

tgatattatt ttgaaaccc 619

<210> 34

<211> 1647

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 34

accgctaccg tcatcactac aaatgctggc tcgcagctta cagcaaatca gacgctcaag 60

caggctgagc ttacaattgc gcgcctacgc cagcagtcca gaccgcagcg caagcttctc 120

taagctctca gagcaagatc tcccatcact cgcctccatc ttctcatccc ccgacacctc 180

cctcctcacc acgctcggcg acaagccaac agccaccagc gacgatctcg agccattcaa 240

cgtcgactgg atgggcaagt acaagggcca ctcttccata attgtgaaac caaagacgac 300

gcaagaagtc agcaaggtgc tgcagtggtg caacgagcgc aacgtagctg ttgttccaca 360

aggtggcaac accggtctcg ttggtggatc cgtgcctttg cacgacgagg tcgtcttatc 420

tctctcctca atgaacagca tcagacactt cgaccctctt tccggttacg tttctgtcga 480

ttccggtatc gtgctcgaaa atttggataa ctacctcgca caacaaggac acattgtccc 540

tctcgatctg ggtgctaaag gctcctgtca gattggtggc aacgtcgcaa ccaacgctgg 600

tggtctgcgc atgttgagat acggtagttt gcacggcaac gtgctcggcc tcgaagtcgt 660

tctgccagat ggtagagtaa tcaatggtat gaagggactc aagaaggaca acactggtat 720

cgatctcaag cagctcttca tcggctcgga gggtgttctc ggtgttatca ctggtgtcac 780

tctcgccaca cccgtcagac catccgcaac taacgtcgct gtcttcgctt tgcctgacta 840

tgagtcagtg cagactgcct tctcatcagc tagacgcgat ctcggtgaga tcttgtcggc 900

gtttgagttc ttcgatgctg cctcatacaa gctcgtgcgc agccatggac acgcagctga 960

gcgcaaaacc ttcgaagatg gggaagacgc accatttttc tgcttggtcg agacgtctgg 1020

ctcgaacaaa gaccacgacg atgagaaact gggtgctttc ctagagcagc tcatggagtc 1080

aggtatcgtc aatgacggtg tattggcaca agacgagacg caaattggcc agctgtggtc 1140

gctgcgtgag ggcattccag aagctgcagg caaagctggt cgcgtgtaca agtacgactt 1200

gagtttacca gtcgagaaga tgtactcgct ggtgccagag ctgcgccaaa agcttgctga 1260

gaagggtctg cttgccgctg agtcagaggg tggtaatgga gatgggccag tcaagacagt 1320

cttcggattt ggtcaccttg gcgatggcaa cctgcacatc aacattgttg ccgatgctta 1380

cagaaaggag gtggaggaag tcgtcgagcc atacatttac gagttggtag ccaagtacaa 1440

tggatctatc tcagcagagc atggtctcgg tctgatgaag gcaccttatg tcgcatacag 1500

tcaagacgcg ccatcgcttg acctcatgcg cactctcaag aagacactcg atccaaaggg 1560

cattctcaac ccatacaagt gcgtcaccgc ggaatagatt ggagttatag atttacgtta 1620

tatgcatgcg atcctgttac attatcc 1647

<210> 35

<211> 669

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 35

gtcatcaact tcattcagca aaatggctct ctattacggc atcgtatttg gtatcttaac 60

atttgagatt attctctttt tcttattctt gttgcctatc ccaactcgtt ggcaaaaacc 120

agtgttccgt tggttagcta cctcacctac cattgcacat gctcaatata tcatgaaaat 180

tgtatttgta ttcatctttg tgctcttcct tgattccgtc aacactctcc gcgctttcta 240

cgaagtagtg aacactgaag atgagaatgg tggtattcca gctgccggta actctgattt 300

cagagctcaa gttggtcaag ctgcaaagaa gttttatgct caaagaaatt tgtatctcac 360

tggattcacc attctgttat tactcatttt gaacaagatc aagaacatgg ctatggacta 420

tattagattg gaagatcaat tcattgagct tgaaggatcc gtttccaaag atcccgccat 480

cagaaaggca agcaaagaaa tcgacactac tcccatcgaa gaccatgtta caagactcga 540

gcctgttgaa caagaacagg aaaacaaaaa ggatatctaa ttcacacctg taactaatat 600

gtaaacatct ccctcgctaa aagcgcaata aactaaaatc agcatcattg cgtatctctt 660

tcttctcac 669

<210> 36

<211> 873

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 36

gtggcgcgcc gggaggcgcc taacggtcat gaattgcctc ctcgcggtta tgatcccgga 60

gaaaacactt accaagcacc acctgatgaa cgtagtcaag tagatgttgc gattgaccct 120

aaatccaacc gtcttcagct gttgaagcct ttccagaagt gggacggcaa ggacatcacc 180

aatgttccta tcttgattaa ggtgcaaggc aaatgcacta cagatcatat ttccatggcc 240

ggcccttggc tcaagtatcg tggtcatttg gacaatatca gtaacaattt cctcattggc 300

gccaagagta gcgaaggcaa agtcaacagc atcaagaatg cttttactgg tgaatacaag 360

ggtgtccaga aacagctcgt gattacaaga aggaaggtgt tcgttgggtc gtggtaggtg 420

atgagaacta tggcgaaggc tcctctcgtg agcatgccgc tctagaacct cgattcctca 480

atggagctgc catcattacc aaatcatttg ctcgtatcca tgaaaccaat ctcaaaaagc 540

aaggaatgct tcctttaacc tttgctgatc ccaaggacta tgacaaggtg gacgcctcag 600

ataaagttga tattcttggc ttgactgatt tccaagaagg aaagccattg acccttcgct 660

tgcacaaaaa agatggatca actgtcgatg ttcctttgaa ccatacattc aacggtcagc 720

aaattgaatg gttcaagcat ggatctgcct tgaaccttat gaaggaaaat actgccaaga 780

acggaagctt gtaggtgcac cgttacgtta tcttcacaag catttgtatg tcaaataaac 840

tcgattagtt acttgcactt ttgttaagtt tat 873

<210> 37

<211> 874

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 37

gtggcgcgcc gggaggcgcc taacggtcat gaattgcctc ctcgcggtta tgatcccgga 60

gaaaacactt accaagcacc acctgatgaa cgtagtcaag tagatgttgc gattgaccct 120

aaatccaacc gtcttcagct gttgaagcct ttccagaagt gggacggcaa ggacatcacc 180

aatgttccta tcttgattaa ggtgcaaggc aaatgcacta cagatcatat ttccatggcc 240

ggcccttggc tcaagtatcg tggtcatttg gacaatatca gtaacaattt cctcattggc 300

gccaagagta gcgaaggcaa agtcaacagc atcaagaatg cttttactgg tgaatacaag 360

ggtgtcccag aaacagctcg tgattacaag aaggaaggtg ttcgttgggt cgtggtaggt 420

gatgagaact atggcgaagg ctcctctcgt gagcatgccg ctctagaacc tcgattcctc 480

aatggagctg ccatcattac caaatcattt gctcgtatcc atgaaaccaa tctcaaaaag 540

caaggaatgc ttcctttaac ctttgctgat cccaaggact atgacaaggt ggacgcctca 600

gataaagttg atattcttgg cttgactgat ttccaagaag gaaagccatt gacccttcgc 660

ttgcacaaaa aagatggatc aactgtcgat gttcctttga accatacatt caacggtcag 720

caaattgaat ggttcaagca tggatctgcc ttgaacctta tgaaggaaaa tactgccaag 780

aacggaagct tgtaggtgca ccgttacgtt atcttcacaa gcatttgtat gtcaaataaa 840

ctcgattagt tacttgcact tttgttaagt ttat 874

<210> 38

<211> 718

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 38

ggcgcgccct tgacacagga gcacgggttt cccgtgcgcg tcatcgttcc aggcgtggcg 60

ggcgcgaggg ccgtgaagtg gttggatcac atcacagtgc agcgggaaat gagcagcaat 120

cattatatgc atttcgacta caaggtccta ccaccagaag cggtcgatgc ggaaagggca 180

cgcaccttct ggcataaagt cccgccggtg atcgacatgc cagcgaattc tgccatcacg 240

tcgccacgaa atgaagacac ggtggaagtg gatgcagagg gatttatcac ggtggatggg 300

tacgctttgc cggggggaga agatgggccg gtgaaaagag tcgaggtctc cattgacaag 360

gagagatggg tcgacgcgga actgtttaca catcccatgg aaagcaagtg gacttggaaa 420

atctggaagg ccaaagtgca ggtcgagccg ggcgagcgaa gatgtctcta cagcagaacc 480

actgatgaag cgggcaactc gcagccgcag cgttctcagt ggaacctgag aggcgtatgt 540

tacaacggct atggagaagt gaggaatttg aaggtggtga aaggataggc ccaatcgttc 600

attccatcat ccatcaagat gtgtctgtat gtgtatgaag gcctgaagcg accacgggac 660

cccagggtgg tcactaaaca gtactcaaac ggactgtttg gttcgtttga cactttcg 718

<210> 39

<211> 160

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 39

aatcaagttc gactgtcaaa atgccagcca acacgatgtc tgccactctc agatccctcc 60

acgttcccgg gaaaccagtc atcttcgcca atgtctggga caccgtctcc gccaaatcaa 120

tcgcacctct ggattcatgc aaagctctag caacggccag 160

<210> 40

<211> 1274

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 40

gccttgccca tgctactcat caaccctcct cgcaacctca tcggccaaag tcagtctggt 60

accggcaaga cggctgcatt caccctcaac atgctctcac gagtcgaccc aaacatcatg 120

acccctcagg ctatctgttt ggcaccgtcg cgagagcttg ctcgacagat tcaggaagta 180

gtcgacaaga ttggccagtt cacccagatc aagagtttcc tcgctgttcc gggctcttgg 240

tcgcgtaatg tcaagatcga caagcacatt cttgtcggta cgcctggtac actcgtcgac 300

atgctttcgc gaggaggcag gatcttcgac ccgaagcaga ttagagtctt tgtgctggat 360

gaagcggacg aaatgatcgc tttgcaaggt ctgggggacc agacgaagcg catcaagagg 420

atgctgccgc ctggggtcca gaacgtcctg ttctccgcta ctttccccga caacgtccga 480

gactttgcag gcgacttcgc acccgaggcg aaccagatct tcctgaagaa agaggagatc 540

actgtcgacg ccatcaagca gctctacctc gagtgtgatg gagaggagca gaagtacaac 600

gccctttctg ccttgtacga catcatgtcg atcggtcaga gtatcgtatt ctgcaagcga 660

aaagacacgg ccgaccgaat tgcggcgaga ctgacggatg agggtcactc tgtcgcttct 720

ctacacggtg acaaacagac tcgagaccgt gatgacatcc ttgacgcttt ccgagatggc 780

aaaaccaagg ttctgatcac caccaacgtc gttgctcgag gtatcgatat ccagcaagtg 840

aacatggtgg tcaactatga cgttcccgat ctcggtccag agggagattg gaagcctgat 900

atcgagacct atatccatcg aataggtcga accggtcgat ttggtcgaaa aggttgttcg 960

gtcatctttg cccatgatca gaggtcgatg caggatgttc agttcatcgc cgatacgctc 1020

ggcaagaaaa tgagcagaat caacgctacc aggcagactg atctcgatca gctcgaagcg 1080

gctttgaaag ccgccatcaa gggcaatcaa ccgaaagagt gaagagtggc accgaattcg 1140

aagagacggg cgctggaaga tatcctgaag caacagggag gagctcccct tatagcatga 1200

tcattgacga taaccatcta gggcctgaag tacattatga tagatagcag acatcaatgc 1260

aacgtcgcgt cgcc 1274

<210> 41

<211> 919

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 41

cacctccaac ctctttctag tttaccttca aaacacatcg gtgtaaggtc ttgcccaaca 60

tggctacctt ctcgacccgc atcaacctcg tccccacctc ccgaacgctc gctagcggcg 120

ttccattcgc acccaggatc gcccttgttc atcctcccgc gtctcacggt cacggaacga 180

gcggtcccag gagtgatgtc ccacccaggt gggctggtgt ccagggtgga ttcgcctcga 240

actcgagggt caatgtactc cccaccggca acttccagca acgattcatg tccaccacgc 300

cagcccgcaa gatcgaggct caaccccacg tccgaggtgt tcccgattgg tcggcatatc 360

agtcttcggg caagggcgag aacacccgat ccctttcgta cttcatggtc ggatctctcg 420

gtgtcctcgc tgcttcaggt gccaagtcga ccgtcagcga cattctgagc aacatggccg 480

cttcggctga tgttttggct ttggccaaga tcgaagttga gatgggtgct atccctgagg 540

gcaagaacct gatcgtcaag tggcgaggaa agcccgtctt cattcgacac cgaacggaag 600

atgagattaa cgaggcacgc gcagtcgaca tcaagtcttt gcgtgatccg gagagcgacg 660

aggataggac ccaaagggga gagtggcttg tcatgctggg tgtctgcact cacttgggtt 720

gtgttcccat tggcgaggct ggtgattacg gaggatggtt ctgcccctgt cacggatctc 780

actacgatat ctctggccga atccgacgag gtcccgcccc tctcaacttg gaggttcccg 840

agtacgcttt caacgacgac gaggagaagc ttgtcattgg ttaggtgtag atggacatat 900

gcagtctatg gccatagcg 919

<210> 42

<211> 1459

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 42

ggggagcatc accatagtga cagggactgt agctcggtca agcgcgtatt cgcacttggg 60

cgcctggacg ttactctggc cgtggagtgc gggagtgtca ggacgatgtc ccttcgtgac 120

ctcgcttgct acactctcac gctcaagcca tctaccgaga ataccctcct gaccgagctc 180

acggctttgg agggaccgag tgaggagcca cgattcgcaa gagtgcggga gaaggtggaa 240

ggagaggtct attcgtctgc catatacgat gcgttgacgg gagccaagct ggcctcggtg 300

ggtttcgctt ccgaaaagca gaagaacagg aggctacagc tacacaaccc ggatgagagt 360

gtgccttttg acaatactag caagctaggt ttcgaatgga cattcatctt cgaaggcaac 420

aagtacaggt ggacgagaga gctatacgga aaagattata tctgctcact agaccggaaa 480

cccgatccaa gggtggagat ctgcctagct cgagacgcag attcgaaagc gcctggacga 540

ctgcagattc tacactacaa catcgaacga ttcccgaacg agatcaagga tttgagggga 600

ctggaaacgc tactcattgc taccctcatg tgcttcgtcg acgcggccga agatcggtcc 660

aattccggtc cgacccgcac ttcgcccttg cctgctaagc cggttgccaa tgctgcagca 720

ggtcaaagcg gcaccagcgc aagtggatct tctgataccc gagcgaaagt tgcgccggtg 780

acagtgccag taatcactgc agaggacttt gaggatgatt gtgacccgaa tgagatactg 840

gtaggaacgg agactgatgt gggcgagcac attgcacgag ctatagcgct tttggaggac 900

ccgaccatgc tgttcattgt cattcgaacg cgaactgcgg ccgcgagctc aagagcgtta 960

gaagtctcct taggggttac aaggttccgg caccgtgagg gcatgagcga gctgcatcaa 1020

tacgtggtag aggaagatcc ggtccggaag ccgaaaccca ttatgcctgc tcagggcctc 1080

aagttgatca acctggatga tcgaccagcg gcacaatcac ccaccaaacc ggaatggtct 1140

gccccaccta acatcgctgt ttacctatca tcgatcgagt tgccagatct cacgcccaag 1200

cccaagcctg tccaggggca cacacggccg ccaactcaag cacctcatgc tcggcctccg 1260

ccgccttctc aactaccaca aaagccgcag ccgcggccac gcccgcctcc atccgatggt 1320

tcaggtagta gtcagactac actcgcttca acgcgaccgc cccaggacga cgggaaggat 1380

tcgagaaagt ctagctttgg aagactcttt ggcaggtagt acgatacact tagcagggca 1440

tatgcaggtg tatcgacgg 1459

<210> 43

<211> 623

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 43

ggcgacgaca acaagaaaac aatacctcac ctgaacatat tcaataatgg cgtcccaatt 60

gatgcccctg gagctgatcg atcgttgcat cggatcaaga atgcgtgtga tcatgaaagg 120

cgacaaagag ttcagcggca cacttctcgg attcgacgac ttcgtcaata tggtgctcga 180

ggatgtcacc gagtacgact acaccggcgc aacgaccaag cttcccaaga tccttctgaa 240

cggcaacaac atctgcatgc tcatcccagg tggcatgccc gagggcgagt catgaatcac 300

ggacatatga tatcccttct tacgtctctt gaaatggcaa agcgagtctg atttaagaac 360

cacacgtgtc atgagaaggc agactatacc gctgtccagt ccaagctgct tgaacaataa 420

ttatcccgac ggagccacga aaacgtgaca agcggaagct cgcattcgca aagcgccggc 480

gcaataaaac gccttgttca gctcgccgac tttgtgcatg catgcagctc gccacacccc 540

gcagatatca ggctgccttc ttgttatcag gtatgcgtgt ttatactcta gcttatttca 600

gctatgcaaa acctatatca tcc 623

<210> 44

<211> 489

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 44

gcaaaggagt tgtcgcctga cgtcaagcct gagccgacat ggtcttgtgg cgaggttgtc 60

aatgtcgtcg atgagcacgg caatgtcatc aaaccgtcag acctctgggt caagatgggt 120

atgcagcagc aggacaatgt ggacaaccta ttgatcgacg acctgtgtga tcagatgagg 180

gccaaggcca aatgcacaga gaacggcgct caattgaatg tcgacgacct gaaccacatg 240

atgtcgtatg acaagtcata taagcagaaa agggtagacg acctcaaaga caagtacggc 300

tggggagcag tctttggccc gaaatgagcc gcctccgcgg gggcaaggtg gacggacgat 360

ggtagacatg aatatgagag caaacagaca tagggtctga gtccagtagt gtgcttgtac 420

caccactgta aatatttgta cgatagccct acaccactta caattgatca tgtaactgtg 480

tgaaccgtg 489

<210> 45

<211> 480

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 45

gggagcctga cccgccgtgc ttggttcatc caatccacct gcgccaccac tggtgatggt 60

ctctacgagg gtctggagtg gctcgccgac actctccgga aaacgaaccg cgattaaacg 120

cgtataatac gaaattgtga tggggaggat tgtgtacgta gcagagcaag agaaatacca 180

cgggaaatct gcaaatgatg gaatgatgat tatggcggga gtttcttcca atgttcttct 240

gcgaggccaa atatcccggc gatgaaaaag aattccctca ccggcatggc atggccatcc 300

tcaggagcaa ggtgtttgtg tttggctcgc cagggctcta tttctcttcg ctatgctatt 360

agcctcattt gttcttttct ctctggcgcc acgtcaaaat tgctggttta tctccttttg 420

attgcatgtt cagtatcggt atgatctcag tataccagca cttgggttga gcattcttct 480

<210> 46

<211> 1139

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 46

acctcacttt gtgcgaatta tcctcctaca gcatcagctc tcttcagaaa gaggctaaat 60

ctatagaccg tccggaacag gttgtcaaca cgcgcgataa gagaagaagg gaatctactg 120

gtagacaaca gatcgatcgc tcttcagcaa acgcaagatg gagaaccttc ttcgtcagat 180

gcaaggggga ggtggtagga tgggtgcacg gccaggccct ggaggcgaaa ctatcctcgc 240

cgacaacggt gaaacagtcc atatttcatc tcttgctcta ttgaagatgc tcaagcatgg 300

acgagcgggt gtgcctatgg aagtcatggg tctcatgctt ggcgaatttg ttgatgacta 360

cactatctcc tgtgtcgacg tttttgcaat gcctcaatcc ggtacgacag tgacggtcga 420

atcagtggat cacgtctttc aaaccaagat gttggatatg ttaaaacaga cgggccgacc 480

cgagatggtc gtcggttggt accactcgca ccccggtttt ggttgttggc tgtccagtgt 540

cgatgtcaac actcagcagt ctttcgaaca gctacatccg cgagcagtag ccgttgtcat 600

cgaccctatc cagtctgttc gtggtaaagt cgtcatcgac gctttccgat ccatcaaccc 660

tcaatcactt gtcgctggac aagagtcgag gcaaacaacg agtaacattg gtcatctgaa 720

caaaccgtcc attcaggctc tcatacacgg tctgaatagg cattactaca gtctggccat 780

cgattacagg aaaacagaag gggagcaggg tatgttgttg aacctgcaca agcggggatg 840

gacagagggt ttgaagatgc gtgatcactc agagatgaag gagggtaatg agaaggcaat 900

caaggaaatg ctctctcttg cctcggccta cacgaaatct gttcaggaag agacgacaat 960

gacggccgaa cagcttaaaa cccgtcacgt aggaaagctt gatccaaaac gtcatttggg 1020

cgaggcggct gagaaagcga tgggtgatca agtgacgcag agtctggcca tgggtgtcct 1080

ggctgagctg tagacgtaga agagggaaga aaggaaacga catgcattgt acatatcgc 1139

<210> 47

<211> 674

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 47

ggacacaccg gtgacgtctt gagcgtctcg ttctcggccg acaaccgaca aatcgtttct 60

gcttcccgag accgaactac caagctctgg aacactctcg gagagtgcaa gttcaacatt 120

gttgacgatg gtcactcgga gtgggtctct tgcgttcgat tctctcctaa ccccgtcatt 180

cccgtcatcg tctctgctgg ttgggacaag gtcgtcaagg tctgggaatt gtccaagtgc 240

aagctcaaga ccaaccacca cggtcacact ggttacatca acaccctcgc cgtttcgccc 300

gacggatcgc tcgccgcatc cggtggaaag tatggcatca ccatgctttg ggatttgaac 360

gatggcaaac acctctactc tctagaggct ggagacattg tcaactcgct cgtcttctct 420

cctaaccgat actggctctg tgccgccact gcttcgtcaa tcaagatctt agacttggag 480

tccaagtcaa tcgttgacga cctcaagcca gacttctccg ccgagtaccc tgacaaggct 540

caaaagccac aatgtacttc cctcgcctgg tctgccgatg gtcagaccct ctttgccggt 600

ttctccgaca acctcgtccg agtctgggtt gtcactgctt agagtcgtga ggattgtatg 660

catggataac gtgg 674

<210> 48

<211> 674

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 48

ccacgttatc catgcataca atcctcacga ctctaagcag tgacaaccca gactcggacg 60

aggttgtcgg agaaaccggc aaagagggtc tgaccatcgg cagaccaggc gagggaagta 120

cattgtggct tttgagcctt gtcagggtac tcggcggaga agtctggctt gaggtcgtca 180

acgattgact tggactccaa gtctaagatc ttgattgacg aagcagtggc ggcacagagc 240

cagtatcggt taggagagaa gacgagcgag ttgacaatgt ctccagcctc tagagagtag 300

aggtgtttgc catcgttcaa atcccaaagc atggtgatgc catactttcc accggatgcg 360

gcgagcgatc cgtcgggcga aacggcgagg gtgttgatgt aaccagtgtg accgtggtgg 420

ttggtcttga gcttgcactt ggacaattcc cagaccttga cgaccttgtc ccaaccagca 480

gagacgatga cgggaatgac ggggttagga gagaatcgaa cgcaagagac ccactccgag 540

tgaccatcgt caacaatgtt gaacttgcac tctccgagag tgttccagag cttggtagtt 600

cggtctcggg aagcagaaac gatttgtcgg ttgtcggccg agaacgagac gctcaagacg 660

tcaccggtgt gtcc 674

<210> 49

<211> 480

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 49

gggagcctga cccgccgtgc ttggttcatc caatccacct gcgccaccac tggtgatggt 60

ctctacgagg gtctggagtg gctcgccgac actctccgga aaacgaaccg cgattaaacg 120

cgtataatac gaaattgtga tggggaggat tgtgtacgta gcagagcaag agaaatacca 180

cgggaaatct gcaaatgatg gaatgatgat tatggcggga gtttcttcca atgttcttct 240

gcgaggccaa atatcccggc gatgaaaaag aattccctca ccggcatggc atggccatcc 300

tcaggagcaa ggtgtttgtg tttggctcgc cagggctcta tttctcttcg ctatgctatt 360

agcctcattt gttcttttct ctctggcgcc acgtcaaaat tgctggttta tctccttttg 420

attgcatgtt cagtatcggt atgatctcag tataccagca cttgggttga gcattcttct 480

<210> 50

<211> 1170

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 50

aaagttgatc gaccacattg ggtctgagaa aaacccaatg cttttttgta cagcagtgag 60

gaatattggt caatggccga aaggctgaac cagtaacttg gaagaatgaa attgtatttg 120

tataaataca atagtggtta aaccacataa aattctaaat agattatata taatgacaaa 180

atctatttat aagtcttgac caaactacgt gccagcagtc gcggtaatac gtagaaggct 240

agtgttagtt atctttattg ggtttaaagg gtaagtagac ggtaaattaa actctaaaag 300

agtacttatt tactagagtt atatgagaga aggaagaatt cctggagtag agataaaatt 360

ttttgatacc aggaggactg tcaacggcga aggcgtcctt ctatgtaata actgacgttg 420

agagacgaag gcttgggtag caaacaggat tagataccct aatagtccaa gcagacaatg 480

atgaatgtca tacattagat agattttaat gtataaacga aagtgtaagc attccacctc 540

aagagtacta tggcaacata taaactgaaa tcattagacc gtttctgaaa ccagtagtga 600

agtatgttat ttaattcgat gatccgcgaa aaaccttacc acagcttgta tagcagttat 660

gaaaaattgt tacaagcgct gcatggctgt ctttagttaa tgtcgtgaga tttggttaac 720

tcctctaatt aacgaaaacc ctcactttat ttatatatat aaagtggttc gctattacat 780

tggttgataa tagggattaa gacaagtcat tatggcctaa atgctgtggg ctatagacgt 840

gccacatacg cctttacaaa gggatgcgat attgtgaaat ggagctaacc cccaaaaaag 900

gaaatactat ggatagtagt ctgtaactcg actgcttgaa taaggaatta ctagtaatcg 960

tgaatcacca tcgtcacggt gaattatttc tcagttaggt actaaccact cgtcaggcgc 1020

tgaaagaaga agatgcagta agtttgatgt tttctgtgta tgattataca taaagttgtt 1080

gtataactac gcagaaaagt tttcgtatgc aaaactttga ttggtgttaa gtcgaaataa 1140

ggttcgtgta atggaaattg cacggggagc 1170

<210> 51

<211> 1231

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 51

ggacgatatg acttcaagca gcctcagcga attcgagacg cttctgtcac ggccacgcca 60

gaatggaacc tgcttgaaga gatcgagttt ggccgattgg gcaagctcaa cctttccgtc 120

gaagagcccg aagacctcga atcgcacggt accctccaag gttacgacaa gacgtttgac 180

cgcatcaaca ctcgtaccga aagacctctc gagatcattg atcgagcatg gtacaatcaa 240

accacttctg acgatcccgt tattgctcag ctcgctcaaa cgcagtctgc ccaaatcttc 300

gcgacagatg ccattcttgc ggttctgatg tgcaccactc gttccgtaaa ctcgtgggat 360

atcattctcg agcgacgagg taaccagctt ttcctcgaca aacgagattc tggtccattc 420

gactacgtca ctgttcacga aaacgccgcc gacccacctg ccgactctga cgatcccaac 480

aacgtaaact cggcttcttc cctttcgctc gaggccacct acattacccg aaatttctcg 540

tctcaagtca ttgatgccaa gtccaagcca tattcgccta gccccaatcc gttctattcg 600

gaggacgagc catcacccgt cgcttcctgc ttgtacaggt accgaaagtt cgacctgtct 660

gttggcgagg aagataccct ggacctcatt gtacgaaccg aagtcgacgc ctatcaaggc 720

aagaaggact ctctcgtcac tgtcaaggca ttgaacgagt ttgatcctcg agcttcaggt 780

ggtggcaaag ccctagactg gcgaaagtac ctcgacactc aaaagggtgc cattgtcgcc 840

tcggaaatga agaacaactc ggctaaactc gctcgatggg ctatccagtc tgtcttggcc 900

ggtgccgaag tcatgaagat gggatacatc tcgcgagctt cgcccaggga tacaactcat 960

cacgtcattg tcggtgtgca aaattacaag ccaaaagact ttgccgctca aatgaatgtg 1020

tccctcaaca acggttgggg tatcgtccga acgattgccg atcttgtcct caagcagcca 1080

gagggcaagt atgtcctcgt caaggaccca aatgcaggca tcattcgtct ctacagtgtg 1140

ccagagaatg ctttcgaggc agaggaggag gaggagcaat agtcgaaaag tctagacagg 1200

ccgtgtcgga catgcatcat atacttcaag g 1231

<210> 52

<211> 788

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 52

gagcgctcaa ggtccttggc cccagaagcc gatcaaggtg tcgcgactca gtgtcacgaa 60

gtcagatcgt caggtagcta cgacacgttc gagatcggcc atcaccaacc tgacagcgat 120

acctcgggag tagctgactt gcgcacttcc agtcgtatgg acacctgcga cgcgcatctt 180

ctacggcggg tcaaaagctg ccctcttttc agctaccgcg aagacgaggt gtccgaaact 240

gtacaattgc ctacaggcga atggacgacg atcagagata tcactccgag tgcaccaaag 300

atcggctttg aagtgcgcga ctcgctctcc gcgttcccga cagccaagcc tgtcgaagcg 360

aagcacgagt ccgcctccag catatccaat gatttaccct ctcagccatc ctcaaggccg 420

ctgattgagt gtccgacact ggtcgccgat tcacgcacaa cgacggggtc caactctgtg 480

cgcagtttcg acgcccagac cgaacgcctg agcggcttga gcgacgtgca ccacagatac 540

atgcaggaca agccgtcaca gcgttctgat tcctggaccg acgtcaaatc ctccgctccg 600

tcctcccagt cgatggcagt ccccaacaaa gcggcttacc tggctccgat cccagctggc 660

ccaaatgaca gtaagacttc gagttccggt cgcgccccgt cagacgccgc gaccgaacac 720

gagtgttcgc tacaataagt cagacttgct gttggaacgt ttcctacctc atgcatacct 780

ggcatgct 788

<210> 53

<211> 706

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 53

accctccaaa ctccaagctc ttttcaaccc tttcctacct tacacaacaa cttcaacaac 60

aactatggca cccaagtcca ctgacaagcc cgcatccacc gctggcaagg ccccctctgc 120

tggaggcaag gctcctgcct ccaagactgt cggtgctaag aagaccgcag caaagaagtc 180

tgctaagtct actggcgagg gcggcgagaa gaagaagcgt gtcaagtcca gaaaggagac 240

ctactctacc tacatctaca aggtcctcaa gcaggtccac cctgacactg gtatctccaa 300

caaggccatg cttatcctca actctttcgt gaacgacatt ttcgagcgta ttgccggtga 360

agcctccaag ctcgctactt acaacaagaa gtctaccatc tcctcccgcg agatccagac 420

tgctgtccgc ctgatcctcc ccggtgaact gtccaagcac gctatttctg agggcaccaa 480

gggtgtcacc aagtactcca gctccaagta aacttgtctt ttgcttggct gagagtcttt 540

cccctttcct tcttcattgt ccctaccctc ctgttcttcc cctctccctc acattcatca 600

tgttgtctat taggcgagct gcctgcagac ttgctcgctg tcaaggctga agcagtcgcg 660

tagttagtgt aatggagcca caaatgtaat tctagagcac atgcag 706

<210> 54

<211> 1203

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 54

gatgtcaagc gattcaccaa ggatctgctg ttcaactcgg agggcaacct aaccttcaag 60

ccccacttgt ggaacgacat ccgtcacacc ctcctcccca cctttatccg acagatcgga 120

tacgttccca tcccacgagc cgagttctcc tcgcctgaca ttgaccttgt catcgagaat 180

ctggtcctgt ccggacccaa cctcttcccc aacgtcgtct cgctcgagag ccacaactcg 240

ttcaagttct cgccttacca gcagctcaac aagggtatgg acacgcatca ccacaagttc 300

aggctgggta tgagccagat ccaggccgat atccgagatg tccgattctc gttccgacga 360

aagactggat ggcccaagct caaggaccac ggtctcgccg atgtcatcct tgccggtaag 420

ggtatgtcga tcgacgtcga gctcgagtct gtcgagggac gacgagactc tgttgtgcga 480

gtcaaccacg tccacaccac catcgacacc ctcaccttct ccatccgaga ctccaagcac 540

gacttgctct acaagttcgt caagtcggtg gccacgggta cgatcaagaa ggcaatccag 600

gccgccgtcg acaatgccat ccgtacggct gtcggtcacc tcgacgacca gctcgtccag 660

gtccgaaaca ccgtcgatga cgccaagaag tctgacgaga ccacccgaac gcaagccctc 720

aaggacttgt actcgaagaa ggcggacacg gcacagaaga agcaggccga gtccaaggag 780

cagcctggta ctttccgaat cgtcgccaac cgagactctg ttctcaaccc cgacatgggc 840

ggtggcaagg gcgccatgac caacaagatg tggaagaccg aggaccttgc acactctggc 900

aaggaatggc actctcccgc tttcgacttg ctcgactcca agcacccagc acgtaccggt 960

cagacccacc ccgaggccaa ggagggtgct ggacacggaa acagcttgag ctcaaaggct 1020

cagcccggcg ccaacgcggc cgaccagctc aaggctactc acggtcagtc tgaggctgag 1080

gccatcgctg gtcagaagcg acagcaatag gtggaagaga gggagccgcg tattgagaag 1140

taggaaggac tagctgtata cccccttata cttttgtgtc tatagtaatg aatgcgtgaa 1200

acc 1203

<210> 55

<211> 962

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 55

ggacctttca tccatcagcg tatcgcatat cagcttcctg acaaagtaag aggtaataac 60

aagacaccac actctttcag cacgtacctc ataccggacc gccatgtaca catccgccgt 120

gacactgctc tctttggtct tgctcctggc gacttccgtt attgcacaag aacaagctgg 180

tcggcctggc actcagcgag gcggcgtctt ctttgggtgt tatgccgatc gacctacagg 240

caatgccaac cagcccatca ctcgagtcgc caactccgac acattctttg aatgcatgga 300

gaattgtgct gcgataacgt ctccttcgtt gctgggatac tatcaaccct cctccggtca 360

atgcttctgc ggcaaccttt tatttaaccc tcaagctcaa ttgaacggta acggttgtca 420

aggtagtgat tggtcctttg gccggacttc gaccaccttc aggaggttcg gtgacgcttg 480

tcgacctttc ggtggtgtcg gattttctgc gaatcaatac actacagtca ctggtcccgt 540

agcttgtcat gttcaatgcg catcaaacag attcgcctat gtctggtccg atactggaag 600

caactcatgg caatgtgctt gcagcaacaa tgtccgtgtt caggaggact tccagtacac 660

ttgtcaaggt ggcggtgtat ttgtgtttga acattcagta caagctcagg cttcttcgct 720

taacaggaag cggacggtgg aggaacaatg ggctgttccg aaagacgccc tctgtccatt 780

cggaatgtca gcgtgcaagg tatcaggtgt cgataatgct tacgaggtat gcttcttttc 840

agaccgctag gcccctggtt ccctggccac gaggtttgaa acacgccatt gacctgtagt 900

gcctcgatac ctcagccgag ctagaatcgt gcggtggttg tctgcatggt caattgttct 960

ga 962

<210> 56

<211> 909

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 56

gggggaggaa cggtcgttag caatgctttg ctggagaatg ccaagctctg caagacccag 60

ggcaaggaga gctctcttcg agtcatcgtg tgtggccgaa ataggttgga gaatgggtct 120

gcacctcatt gggccgaggc gtttgctacg catggcaaat tggtggaagt gaggatgccg 180

caaaacggca ttcgcatgga gggcatcaaa gctatcgccg acggactggc caagtgtccg 240

acattggaag tgcttgattt gcaggacaac acggctacca agacaggaac acggagtatt 300

gtccgacacc tctcaacttg gcctaaactt cgaatactca atctctcgga ctgtcttttg 360

ggttcggtcg gcggtatcgc tcttgcaacc gcattgtcca ctggctcgaa caagcacctc 420

gaacagctca aactgcaata tggcgagttt gacaagagga cggttgagat actgtcgacg 480

gcaattagcc agcatttgcc aaaattgacg acactcgaac tgaatggaaa ccgtttcgat 540

gccgaagacg aatgcgttga gaccctgaag aaggcacttg agctacatgg gaacgaggat 600

gctttggacg aacttgacga tatggaggag gtggacgagg acgaagagga tgatgatgac 660

gaggacgagg aggacgaaga cgaggacaag gacactagcg ccgacgatgg gatcgatgca 720

ggagctgctg gagaagacgc tctaccacca gtcacgaaga aggacgagga cgtacttgcg 780

gatctcctgt ccaaggtcca cgttcagcct agctgagtcc aagcgctttc cggtcggcaa 840

gtagatagac tagacagcat aataccttga ccctcatgat gccacccgca tgtacacatt 900

tgttctccg 909

<210> 57

<211> 909

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 57

cggagaacaa atgtgtacat gcgggtggca tcatgagggt caaggtatta tgctgtctag 60

tctatctact tgccgaccgg aaagcgcttg gactcagcta ggctgaacgt ggaccttgga 120

caggagatcc gcaagtacgt cctcgtcctt cttcgtgact ggtggtagag cgtcttctcc 180

agcagctcct gcatcgatcc catcgtcggc gctagtgtcc ttgtcctcgt cttcgtcctc 240

ctcgtcctcg tcatcatcat cctcttcgtc ctcgtccacc tcctccatat cgtcaagttc 300

gtccaaagca tcctcgttcc catgtagctc aagtgccttc ttcagggtct caacgcattc 360

gtcttcggca tcgaaacggt ttccattcag ttcgagtgtc gtcaattttg gcaaatgctg 420

gctaattgcc gtcgacagta tctcaaccgt cctcttgtca aactcgccat attgcagttt 480

gagctgttcg aggtgcttgt tcgagccagt ggacaatgcg gttgcaagag cgataccgcc 540

gaccgaaccc aaaagacagt ccgagagatt gagtattcga agtttaggcc aagttgagag 600

gtgtcggaca atactccgtg ttcctgtctt ggtagccgtg ttgtcctgca aatcaagcac 660

ttccaatgtc ggacacttgg ccagtccgtc ggcgatagct ttgatgccct ccatgcgaat 720

gccgttttgc ggcatcctca cttccaccaa tttgccatgc gtagcaaacg cctcggccca 780

atgaggtgca gacccattct ccaacctatt tcggccacac acgatgactc gaagagagct 840

ctccttgccc tgggtcttgc agagcttggc attctccagc aaagcattgc taacgaccgt 900

tcctccccc 909

<210> 58

<211> 596

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 58

ggatggtgaa gcttagtaac agtcttgtcc gtcgtcttaa atggcaacac gttcgcagtc 60

tcggcgtggt ggcgctgact gcccaattgc gaggaccaca acctcagagc gccgaggacg 120

aagattctga agcagctggc aagaagctca aactggctgg cgaccaagct acatctgcgg 180

tcattcccaa gtccgcagac aagcccgata ctttccctct actcgacaca ctacctgcta 240

ctatggctgc tggcaccagg tctatgacta ggcccttgca tgtcggtgat ctgaggttgg 300

ctgatctgcg taaaatcatg caggcagctg gccacacggc tgagttccga ggtgagggaa 360

cactactcat tgacaagtcc gtcgctgtca gaaaatcagg cacagggcag attgaaatcg 420

aggcatctgc tcaagcagct gcaaaccaag ctactcctgg ccgaggtgcg agtagcttcc 480

tcgctgtcaa aagaaagata tacgagggtc tcgctgttgt cacaggaagt taaatgaccg 540

tgtaccctat attcaatttt tgtataattt acgcaatacc aacgatattc tctcgt 596

<210> 59

<211> 418

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 59

gaaaatgaaa attgatgtgg agaagctgaa taaagatatc agccttttcc cgcaggtgca 60

tccgattacg gaagatatga aaatcacgca caaaggtgtt tcgcgccttg taatgctgga 120

caggtattca tttaaagaca ctgaaaaaat tacgctatct gaaggcgatt ttgtagtgct 180

gacgatcaag gaagatccaa aatttcctgc aagagggcta ggctacatta aagaaattga 240

ttgggaaaat aaaaaggcaa aggttcaggt cgaagaagag tttcgtcata ctcttgaaaa 300

gcctgaagaa cgggagacgg gaatcatcgt tcgctcttta gatgtcatcg aaaaaccgct 360

tgaaattttt tatgaacaaa ttgccaaaag aaatgcaaca ggtcttgctg ctgttgaa 418

<210> 60

<211> 988

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 60

ggggatgcaa cggtgactca actgcgcgaa atcatggacg acccagctgg ctatttcttg 60

ccaaatctca aacatggcgc cgataacatg ttctacgtcg gtccacgcgg acttgcacaa 120

gagctcgagg agctttttac cttcccaagc acaatcctca gaaagcgcca ggataccagt 180

cagcatgacg aaaggcaggc aaagaaggcg cgcacgcaag aggacgaagc ggctggtgac 240

gcgttggagg agcccgagac tgggcgacgc gacagtgtgc ttccgactga acgggccgct 300

tttggtctcg agggtgatga ctcgggcttt ttccttggcg accagacgat gggagacgac 360

atgctgccta tggacgacat gggagccatg gacaccggag tggaccagcg acgcatgcga 420

acaccatcag tcgcaccgtc ggtcaccgaa tcgatcgcac gtcagattca gaatgaccga 480

agcgctggca cacacccact ggctatattc gagaaggagg caagggacga cacgcagtcg 540

caatcgcagg ctacgcccaa caaatcggtg gcctccgagt ctatcagcaa gacttcttct 600

ggccaatcaa agaatactgg catggccatg ggtttgttgc gaagggagat tgaggcgatc 660

gaggaggaag acaagatggt cgggtttgat cacttggcag acaaggcgtc caagcgagca 720

gcgtctgcat tcttcttcga gctgttggtg cttggtacca aacatgcggt caagcttgaa 780

caagctcagg ctttcggcga catccacata cgcggcaaag acaagctgtt tgcagaggtt 840

gttgcataga caaacttgaa gagccacgat cttacgcgca acggagggag atctaatgac 900

catcttgatg tcgactttaa tgttatttgg tacttgtaca tgagctgcta agagggtctt 960

gaatgagatg atgcatcgct tcatgagg 988

<210> 61

<211> 614

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 61

gcagaccgtc tcttttaaat ctcctccttg acacccgtct cctttgcaca tttactacac 60

tccacatatc tccataacaa ccttatatct ttacacaatg ggtgaccacg ccactaccaa 120

cgacccctcc aacgccacct tcgaggagaa gggcaagggc aaggacgtcc aggatcaaat 180

cgcggaggac tccagcgacg aggagagtga ccaggagcct gagatggttg acgaggaaga 240

ggatgacaac aacctcgagc ccatctccca agacaacatc atctcaggtg gtcgccgtac 300

acgcggcaag atcatcgatt atgccgccga agccgagaag aacaaggatg agatggagga 360

ctctgaggat gacgaggatt accaaggcgc taatgacgac gaggatgacc agatgcgcga 420

ctaagcgcat ggtcttgatg acggatctca attaacatag gactttggag gattggcgct 480

atggtttctg aaggaggttc tctcgtgcgc ctttgtggtt agcatctcac ctatgaaatg 540

tcatggcctg agcctggcaa tggacatgac tatgaataaa tgaaatgaag cctgcttctg 600

tctttgtgta acag 614

<210> 62

<211> 418

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 62

gaacttaagt attttaaagc agttgcacta tacaacctga gccggtactt ggatgcacgg 60

aaagcaatca atgacctcat tcagagctac ccggacttcc ggcaagctga ggccctcaag 120

tcagccattg atgacaaggt ggtgcgcgat gggctgattg gcgtgagtgt ggcaggagca 180

gtggtggctg gcgtcgtggg cttggctgtg gctcttgcac gtggcaacag aggatgatgc 240

tacaggaggc agcaggttgt tggacagttc agtgcaccgt gccaatgctt caacggtctg 300

gcacaggagg cagcaggttg tgaccctgca caagcttggg ccatgattct acagacacac 360

cttatggcaa tcaaatgtgt gtttgcatgt gcgttgaaga gtgtaaatgt gctcttcc 418

<210> 63

<211> 94

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 63

tatcccgagt agcatgggac acgtggaatc ccgtgtgaat cagcgaggac cacctcgtaa 60

ggctaaatac tcctgggtga ccgatagcga aaaa 94

<210> 64

<211> 371

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 64

cacgatttaa atacccgggg gacgttttgg attcgacagg gatagatcga gcttaagctg 60

cgagccggag ggatcgtctc cgtcatcaac gtcgcctaaa gataactggc aaacaaaaca 120

actacgcttt agctgcttaa tgctaaaggc tcctttcttc catcgcccat gtggaggaaa 180

aggggttcaa cttaagtggg ctacgcccga ttccgccgtc tgaggaagag ggaagagacg 240

aatcagacta gctgtccgga tgcctgccga caggctaagg aacagtgaaa tgttaaatat 300

gtcggatacg ctcgtagatg cttaagtggc gatatctctg gacgtgggtt cgattcccac 360

cgtctccacc a 371

<210> 65

<211> 681

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 65

gccatcagca ccgcaaagct acctcatcaa ccattgaaag cacgcaaata actccccaaa 60

gttaatgccc gtacgaccct acctggaaga aatggccgac atgcccgtgc ccctgttcgc 120

gtacgacgca ccgcccaccc tggccgacca ccctcacgcc cgcgagcacc aacacacgac 180

cttcatgcaa taccttgcgc gcaagcagcc ggacccaaag aactacccca actaccctga 240

cgtggacatc cgcgacgcca tcaatcacta cctgatcgag ctcgaatgcc cggggatcaa 300

agacgcagcc gacatccact gccagtggac gagctcgcgg cacctgaccg tcaccggcga 360

catcgcccgt cctgaggaaa gccagatcga agcgcagatc gagagcaggc ccgtctacct 420

ggttctggga gagagacgca ttggctcttt ccgtcgcaac tttaccttcc ctgtggaggt 480

cgagcaggaa aatatgactg ccaagttgga ggccggattg ttgaagattg tcttgcccaa 540

gcacaagcac catactccga agggaacagg aaaggtcgac attgatgtca ttgagtgaac 600

gtcttttggg tctgcgatta tatgcgagga gttcttagat tgccggagtg ggtacctgta 660

tgggaattat gtatctgcaa c 681

<210> 66

<211> 681

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 66

gttgcagata cataattccc atacaggtac ccactccggc aatctaagaa ctcctcgcat 60

ataatcgcag acccaaaaga cgttcactca atgacatcaa tgtcgacctt tcctgttccc 120

ttcggagtat ggtgcttgtg cttgggcaag acaatcttca acaatccggc ctccaacttg 180

gcagtcatat tttcctgctc gacctccaca gggaaggtaa agttgcgacg gaaagagcca 240

atgcgtctct ctcccagaac caggtagacg ggcctgctct cgatctgcgc ttcgatctgg 300

ctttcctcag gacgggcgat gtcgccggtg acggtcaggt gccgcgagct cgtccactgg 360

cagtggatgt cggctgcgtc tttgatcccc gggcattcga gctcgatcag gtagtgattg 420

atggcgtcgc ggatgtccac gtcagggtag ttggggtagt tctttgggtc cggctgcttg 480

cgcgcaaggt attgcatgaa ggtcgtgtgt tggtgctcgc gggcgtgagg gtggtcggcc 540

agggtgggcg gtgcgtcgta cgcgaacagg ggcacgggca tgtcggccat ttcttccagg 600

tagggtcgta cgggcattaa ctttggggag ttatttgcgt gctttcaatg gttgatgagg 660

tagctttgcg gtgctgatgg c 681

<210> 67

<211> 50

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 67

gggatgatat gcatcatata gatcttgaga aatcaaggta agtatacaaa 50

<210> 68

<211> 558

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 68

gggaaaaaaa ctttagaata cagtttaatc aatcttcaca gctacaaggc tatatcattt 60

gatatagcat atcaaagtgg ctttgatttc tgtaaattta tatctaataa taatagtgtt 120

tatatcagct aaatacatat ttctatccta tctatatatc accgacagac catatttgaa 180

actgctgttg acactattat tcatatgttc ggatttaatt ttaatacgac aaaattgtta 240

aaaacaattc tcgttgtttg ttatttgcag gcaacagtgt tagctgatcc ttatacaaga 300

gtatcttggg aagcgtatat gaatcatgtc aatggatccg acgactatcg tactcaaggg 360

gatgatacca gagctacacg ctttccagag actaaacctc caaaacaagg aaaagatttc 420

ctgtggtcga gtaaaccagt ccccagttca gatctatttc tggagttctt tatgtatgag 480

ggagaaccag atgaattcag caggacgact gaatcgtatc aatcacttcc gagcaacgcg 540

ttaactgcta ggcaaaaa 558

<210> 69

<211> 1164

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 69

ggacacatca cacaacaaca atgtctccaa caccaacatc accacacaac aagctctcgc 60

tccccgcaag agcttcttcc cacgactcga cagacggcat ccgtaagcga gtatgcaagg 120

cttgcgacag gtgtcgattg aagaagagca aatgcgacgg atcaagtcca tgctctcgat 180

gcaaagcaga caacgccatc tgcgtgttcg gagagcgcaa acgatcacat gataaacact 240

atcccaaagg ctatgtcgag atgctcgaac aacagcaggg tcagctcgtc tcaggcctca 300

aagaaatgta ccacagactc cagaaagcct ccgcctggga tggccctgtg ttggacgaaa 360

gcaccggaca gcctctcact cacgacatcc tgtcagcatt agacctcctc gaaccaaagc 420

atgacgacag caacgagcca gaagtcttcg aagagaactg cgaaaagctg caatcaaaat 480

tgctcgcaga cggcgcgggc tttgcccacc gacgaggatc gatcagttcg gattctgaac 540

acagccatca cgatcgaccc aaaacatcct cacgccacga cacgcccgtg caacccaaac 600

cgtcgatctt caaggagaac ctgagcttcg ccagcgcggc ctcatcacca ctcacgcaaa 660

gccccatccc tcgatcgaaa cccttgaacg tcatgccata ccaaacgctg caaccgtcgt 720

caagaccatc cccactccag atgccctcag catacaacga cccgcaacta tacgcacccg 780

aatgggcaca agcactggca gacatgagcg gcgatcccaa ctaccgccaa agattctcca 840

tgcagcagca acaacaaaac gacttcgaca acctgctctg ggatccctca gcgcaagcgc 900

ccatggaatc gcccttcagc caaccagcct tcttcaacca ggcgcaactg atcggcagcg 960

gcaacgtctt tgggctgtct gacatcaacg atctgggccc caaccccgcg gatggcggga 1020

tggactttga cttcagcaag ttcgtgcagc agaccgaagt catgacatga acatgattct 1080

tgccttctgt caatacgcgc gagaattttg cttcagagtt ccagtccgtg taattcttgt 1140

gtatttatta cgatacgaac acgc 1164

<210> 70

<211> 923

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 70

agagctcttc gtactccaca ccaccatctt ccatccgacc acactttcat cccaaatcca 60

tcaacaaccc atctcaactc catctcacca cctcaccatc atcacaatgt cttccttccg 120

cgtcgccgcc cccaagatgg cctccatggc cgctcagtcc tccgtgaagg tcgcccgccc 180

ggccttccag gctgctcagc tccagaagtt cacccgcgcc tactccgcgg tccccaagaa 240

caccgtcttc aacaccatga agcgcaccca gatgatggcc cgccaggcct cccccatcgc 300

caagcgtgcc tactcctctg agatggccaa cgccctcgtc caggtctccc agaacatcgg 360

tatgggttcc gccgccatcg gtcttgccgg tgctggtgtc ggtatcggtc tcgtcttcgc 420

cgccctcatc caggccgtcg cccgcaaccc ctccctccgt ggccagcttt tctcttacgc 480

cattcttggt ttcgctttcg tcgaggccat cggtctcttc gacctcatgg ttgccatgat 540

ggccaagttc ttgtaaaaat gtgcattcca ttacctaccg agatggagat ggatgcgaag 600

gcgattgggg acggagacag tgcgttgctg cagcagcatt agtaccggtg ttggtcgtgt 660

accagtagtc tgatggagac ggatagatgg atagaaagct ggtgaatggg ggctacgaag 720

aaaacgtacc tctcgatcca tttgtaccca tactcatgaa gtatatccgt cttctttcct 780

tctatcattc gcgcgcactt ccttgctggt ggctttttgg ggttgcgctc tcaccgaaaa 840

gcaacgtcac tcttgtatat aacttattcg accacggcca tatcttggtt tggctgggga 900

aataacaatg tctcatttgt acc 923

<210> 71

<211> 923

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 71

ggtacaaatg agacattgtt atttccccag ccaaaccaag atatggccgt ggtcgaataa 60

gttatataca agagtgacgt tgcttttcgg tgagagcgca accccaaaaa gccaccagca 120

aggaagtgcg cgcgaatgat agaaggaaag aagacggata tacttcatga gtatgggtac 180

aaatggatcg agaggtacgt tttcttcgta gcccccattc accagctttc tatccatcta 240

tccgtctcca tcagactact ggtacacgac caacaccggt actaatgctg ctgcagcaac 300

gcactgtctc cgtccccaat cgccttcgca tccatctcca tctcggtagg taatggaatg 360

cacattttta caagaacttg gccatcatgg caaccatgag gtcgaagaga ccgatggcct 420

cgacgaaagc gaaaccaaga atggcgtaag agaaaagctg gccacggagg gaggggttgc 480

gggcgacggc ctggatgagg gcggcgaaga cgagaccgat accgacacca gcaccggcaa 540

gaccgatggc ggcggaaccc ataccgatgt tctgggagac ctggacgagg gcgttggcca 600

tctcagagga gtaggcacgc ttggcgatgg gggaggcctg gcgggccatc atctgggtgc 660

gcttcatggt gttgaagacg gtgttcttgg ggaccgcgga gtaggcgcgg gtgaacttct 720

ggagctgagc agcctggaag gccgggcggg cgaccttcac ggaggactga gcggccatgg 780

aggccatctt gggggcggcg acgcggaagg aagacattgt gatgatggtg aggtggtgag 840

atggagttga gatgggttgt tgatggattt gggatgaaag tgtggtcgga tggaagatgg 900

tggtgtggag tacgaagagc tct 923

<210> 72

<211> 1368

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 72

ggaggattct cggtcaagtt caggaccgca gaaggaaatt gggactttgt ggccaacaac 60

acccccgtct tcttcctccg agacccggcc aagttccccc acttcatcca cacccagaag 120

cgagatcccg ccacccactt gtctggtgac gatgactcga ccatgttctg ggactacctg 180

tcgcagaacc ccgagtcgat ccaccaagtc atgatcctca tgggtgatcg aggtatcccc 240

aagggctggc gattcatgca tggctactac ggccacaccc tcaagattgt caatgacaag 300

ggcgaatggg tctacgccca gttccacctc atctctgatc agggcaccca gaacttcacg 360

ggtgacgagg ctgctcagca atccaacgat tacggacaga aggatctgta cgaagccatc 420

gagaagggag acttcccctc gtggacgatg aaggttcaga tcatgaccga gaagcaagcc 480

gaggaggcat gggagcaaaa gaggatcaac gtctttgatt tgacccacgt ctggcctcat 540

ggtgattacc cacttcgaac agtcggtaaa ttcaccctta acgagaatgc caagaactac 600

ttcgccgagg tggaacaagt cgcattcaac ccgtctcaca tgattcccgg tgtcgagccg 660

tccaacgacc cagtgttgca gtcgcgactg ttctcttacc ccgatgctca ccgacaccga 720

atcggagcca actatcagca actgcccgtt aaccagaatg tgtgcccctt cgccttgggc 780

aacttccagc gagacggcca gatggcattc tacaatcaag gtagtcgacc caactacctt 840

tcttcgattg agccaatctc attcaaggag agggcgtatg atctcaacaa ggtccacggc 900

aaattcgtcg gagaagccgt cgccttcttg tctgaaatca ggccagagga cttcaatgcc 960

ccaagggcac tgtggcagaa agtctttagc gaggaaagca agcagcgatt cgtcgacacc 1020

gtctctggtc acatgtcgac agtccgagac aaagccatca ccgctcgaat gatgactatc 1080

ttccgagaag tttcgcctga tcttggtgat cgacttgaga aggccactgg tgtcaagggc 1140

gaatccacca ttgccgggat gaagttcaac ggaacgcaca atgggtttga caaggccaac 1200

aagatcccgg ctaatgggat gaagaagggt ggagaagtca tctttgacaa tggtgcacct 1260

gctactgctg ccaggtaaat gagcggtcag gcgtacttga tatatgttgt tacgatatgt 1320

cggtctcgta gtcatgtagc caggataaat gaagcggatg tggcagtg 1368

<210> 73

<211> 1368

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 73

cactgccaca tccgcttcat ttatcctggc tacatgacta cgagaccgac atatcgtaac 60

aacatatatc aagtacgcct gaccgctcat ttacctggca gcagtagcag gtgcaccatt 120

gtcaaagatg acttctccac ccttcttcat cccattagcc gggatcttgt tggccttgtc 180

aaacccattg tgcgttccgt tgaacttcat cccggcaatg gtggattcgc ccttgacacc 240

agtggccttc tcaagtcgat caccaagatc aggcgaaact tctcggaaga tagtcatcat 300

tcgagcggtg atggctttgt ctcggactgt cgacatgtga ccagagacgg tgtcgacgaa 360

tcgctgcttg ctttcctcgc taaagacttt ctgccacagt gcccttgggg cattgaagtc 420

ctctggcctg atttcagaca agaaggcgac ggcttctccg acgaatttgc cgtggacctt 480

gttgagatca tacgccctct ccttgaatga gattggctca atcgaagaaa ggtagttggg 540

tcgactacct tgattgtaga atgccatctg gccgtctcgc tggaagttgc ccaaggcgaa 600

ggggcacaca ttctggttaa cgggcagttg ctgatagttg gctccgattc ggtgtcggtg 660

agcatcgggg taagagaaca gtcgcgactg caacactggg tcgttggacg gctcgacacc 720

gggaatcatg tgagacgggt tgaatgcgac ttgttccacc tcggcgaagt agttcttggc 780

attctcgtta agggtgaatt taccgactgt tcgaagtggg taatcaccat gaggccagac 840

gtgggtcaaa tcaaagacgt tgatcctctt ttgctcccat gcctcctcgg cttgcttctc 900

ggtcatgatc tgaaccttca tcgtccacga ggggaagtct cccttctcga tggcttcgta 960

cagatccttc tgtccgtaat cgttggattg ctgagcagcc tcgtcacccg tgaagttctg 1020

ggtgccctga tcagagatga ggtggaactg ggcgtagacc cattcgccct tgtcattgac 1080

aatcttgagg gtgtggccgt agtagccatg catgaatcgc cagcccttgg ggatacctcg 1140

atcacccatg aggatcatga cttggtggat cgactcgggg ttctgcgaca ggtagtccca 1200

gaacatggtc gagtcatcgt caccagacaa gtgggtggcg ggatctcgct tctgggtgtg 1260

gatgaagtgg gggaacttgg ccgggtctcg gaggaagaag acgggggtgt tgttggccac 1320

aaagtcccaa tttccttctg cggtcctgaa cttgaccgag aatcctcc 1368

<210> 74

<211> 1377

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 74

ggacaccatt gacgcagagg tgctcgacag tttgggtgtc acccaagaga acttccagtt 60

tgcccttggc gtcagcaacc cctctgccct tcgcgaggtc gcagtggtcg aggttcccaa 120

cgtcagatgg gaggacattg gtggtctcga ggaggtcaag agggagctca tcgagagcgt 180

gcaatacccc gtcgaccacc ccgagaagtt cctcaagttt ggcatgtccc catcaaaggg 240

tgtgcttttc tacggtcccc ctggtactgg taagactctt ctggccaagg ctgtcgccaa 300

cgagtgcgcg gccaacttta tttccgtcaa gggtcccgag cttctctcca tgtggttcgg 360

tgagtctgag agcaacattc gtgacatctt cgacaaggct cgtgctgccg cgccttgcgt 420

tgtcttcctc gacgagctgg actccatcgc caagtctcgt ggcggttctc agggcgatgc 480

tggcggtgct tccgaccgtg tggtcaacca gcttctcact gagatggacg gtatgaccag 540

caagaagaac gttttcgtca tcggtgccac caacaggcct gagcagctcg acaacgctct 600

ctgccgtcct ggtcgtctcg acactctcgt ctacgttccc ctgcctgacc aggagggccg 660

tgagagcatt ctcaaggccc agctccgcaa gactcctatc gccgacgaca tcgacctttc 720

ctacatggcc tccaagactc acggtttctc tggtgccgat cttggcttca tcacccagcg 780

tgccgtcaag ctggccatca agcagtctat tgacctggcc atccagaacc aaaaggctag 840

agaggccgag ggtgacaccg ccatggacga ggacatcgag gaggacgacc ccgtgcccga 900

gctgaccaag gctcactttg aggaggctat gagcatggct cgtcgctccg tcaccgacac 960

cgagatcagg cgctacgagg ctttcgccca gagcatgaag agctccggtg gcggcagcgc 1020

tttcttccgc ttccctgaga gcggtgccga tggcaacgca gccgagcagc agcaaaatgg 1080

tgctggcgag gaggacctct acgactaaat tggtttcacg aacctcacga cctaatcctt 1140

tgctgttatc ggagtaatat tccagatgga gagagcaatc atgcattcag gcgcgtctat 1200

ggactgaagg ggaagatgga tagagtgttc cagtagccct tttctctttt tttctgggaa 1260

ctcttgctgt ttggctggtc gcctcttatc gagtgtggtt gtgctagagt aggcagttca 1320

gagtttccct gttatgttat gcctttccgg gcagtatgag aataatttcc ttgcaaa 1377

<210> 75

<211> 1377

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 75

tttgcaagga aattattctc atactgcccg gaaaggcata acataacagg gaaactctga 60

actgcctact ctagcacaac cacactcgat aagaggcgac cagccaaaca gcaagagttc 120

ccagaaaaaa agagaaaagg gctactggaa cactctatcc atcttcccct tcagtccata 180

gacgcgcctg aatgcatgat tgctctctcc atctggaata ttactccgat aacagcaaag 240

gattaggtcg tgaggttcgt gaaaccaatt tagtcgtaga ggtcctcctc gccagcacca 300

ttttgctgct gctcggctgc gttgccatcg gcaccgctct cagggaagcg gaagaaagcg 360

ctgccgccac cggagctctt catgctctgg gcgaaagcct cgtagcgcct gatctcggtg 420

tcggtgacgg agcgacgagc catgctcata gcctcctcaa agtgagcctt ggtcagctcg 480

ggcacggggt cgtcctcctc gatgtcctcg tccatggcgg tgtcaccctc ggcctctcta 540

gccttttggt tctggatggc caggtcaata gactgcttga tggccagctt gacggcacgc 600

tgggtgatga agccaagatc ggcaccagag aaaccgtgag tcttggaggc catgtaggaa 660

aggtcgatgt cgtcggcgat aggagtcttg cggagctggg ccttgagaat gctctcacgg 720

ccctcctggt caggcagggg aacgtagacg agagtgtcga gacgaccagg acggcagaga 780

gcgttgtcga gctgctcagg cctgttggtg gcaccgatga cgaaaacgtt cttcttgctg 840

gtcataccgt ccatctcagt gagaagctgg ttgaccacac ggtcggaagc accgccagca 900

tcgccctgag aaccgccacg agacttggcg atggagtcca gctcgtcgag gaagacaacg 960

caaggcgcgg cagcacgagc cttgtcgaag atgtcacgaa tgttgctctc agactcaccg 1020

aaccacatgg agagaagctc gggacccttg acggaaataa agttggccgc gcactcgttg 1080

gcgacagcct tggccagaag agtcttacca gtaccagggg gaccgtagaa aagcacaccc 1140

tttgatgggg acatgccaaa cttgaggaac ttctcggggt ggtcgacggg gtattgcacg 1200

ctctcgatga gctccctctt gacctcctcg agaccaccaa tgtcctccca tctgacgttg 1260

ggaacctcga ccactgcgac ctcgcgaagg gcagaggggt tgctgacgcc aagggcaaac 1320

tggaagttct cttgggtgac acccaaactg tcgagcacct ctgcgtcaat ggtgtcc 1377

<210> 76

<211> 699

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 76

gaaggtgacg acgagcagac tttgcgcccc acgacgatac taacgtaacg acccagcaca 60

cattaatcca caatgggtca ctccgccggt ctcaggaagg gcactcgcta tgccttctct 120

cgcgacttca agaagagggg catgatcccc ctctccacct accttaagca gtacaaggtc 180

ggcgacatcg tccacgtcgt ctgcaacggt gccgtccaga agggcatgcc ccacaaggac 240

ttccacggca agactggtgt cgtctacaac gtgaccaagt ccgccgtcgg cgtcatcctg 300

tacaagcagg ttggcaaccg ttacatcgag aagcgcgtca acctccgcat cgagcacgtc 360

cgcctctccc gctcgcgtga ggagttcatc gtccgcgtca agaccaacgc tgagaagaag 420

cgcaaggcca aggaggaggg caccaccgtc ttcctcaagc gccaggccga caagccccgc 480

gaggcccgca ccatcagcgc caaggacaac aagcccgaga gcatcgctcc tatcgcctac 540

gacacccaca tttaagcgtg cttgtttcga aagggagggc gtacgggctg gtatgatggc 600

gaggctagga ggttggtatc ggcggatcgg attccaccgg atgggaaata cctgccggat 660

gagccagcta gcttcgcaag gtgcatgaat tctagcgcc 699

<210> 77

<211> 1664

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 77

ggggacatgg gcatcggtgg tcttgatacg gagttctcgg ctatcttccg acgagcattt 60

gccagtcgta ttttcccgcc gggactggtc gagaaattag gtatccagca cgtcaagggt 120

atcttactgt ttggcccgcc aggaacagga aaaaccttga tggcacggca gatcggaacg 180

atgctcaacg ccagagagcc taaggtggtc aacggtcccg aaatcctcaa caagttcgtc 240

ggtcagagtg aggagaatat cagaaagctg tttgccgatg ctgagaaaga gcaaaaggaa 300

aagggggatg aaagtggctt gcacatcatc atcttcgatg agctggacgc tatctgtaaa 360

cagcgaggat ctacaaacag cggtaccggc gttggagact cggttgtcaa tcagctgtta 420

tcgaagatgg acggtgtaga tcaactgaac aatgtcttga tcatcggtat gactaatcga 480

atggacatga tcgacgaagc gctcctccga cctggacgtc tggaagtcca cattgagatc 540

tcgttgcctg acgaagctgg ccgattccag atcctcaaca ttcataccaa caagatgagg 600

acgaatggtg tcatggacag cgatgtggat ctgggcgaac tagcggccct gacgaagaac 660

ttctcgggtg ccgagattgg tggtctggtc aaatcagcga ccagtttcgc tttcaaccgt 720

cacgtcaagg ttggctccgt cgccgcgttt gatgatatcg acaatatgaa gatctcacga 780

gccgacttcc tccacgccct agacgaggtt acacctgcgt ttggtgtctc cgaagaagag 840

ctgcaacagg tcgtgcagaa cggtatcatt cactactcgc aacacgtcaa tgacacacta 900

aacgatggaa gtctgcttgt ggagcaagtg cgaaaatccg accgcacccc gcttgtctcg 960

gccctccttc acggtccatc tggcgcgggc aagacggctt tggcagccac gatcgccatg 1020

gcatccgagt tccctttcat caagctcatc tcgcctgaaa caatggttgg gttttctgag 1080

ccgcagaaga ttgctcaact caacaaggtg ttcacagaca gctacaagag tccgatgagc 1140

atcatcgttg tcgacagtct cgagagattg ctggactgga acccgatcgg acccaggttc 1200

tcgaatggtg tgcttcaggc tttggttgtc ctctttggca aacgtccgcc caagggtcgg 1260

cgtcttctca ttctggccac cacgtcaaat cgcaacatcc tcacggatat ggacgtcctt 1320

tcggctttcg acactgatat ccccattaac cccatctcat cgatcgatgc agtggtgcac 1380

gttctagatg aggtcaagtt attcccgaac tcgaaggaaa agcagagagc aacacagatg 1440

cttcgcgagg cgagactggg cgaaggtggt cgaccagact tgttggtcgg agtgaaaaag 1500

ctgttgagta tggcagagat ggcccggcag gatccggatc ccacgatgaa gatcgtgacg 1560

agcattctca gggaggcgag ttaggatgtg tgaagcgtga tcatgataga gtgtagtcca 1620

aacaatgtac tagtgcaaca gaagctatgc agatgaataa cgtt 1664

<210> 78

<211> 1368

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 78

ggaggattct cggtcaagtt caggaccgca gaaggaaatt gggactttgt ggccaacaac 60

acccccgtct tcttcctccg agacccggcc aagttccccc acttcatcca cacccagaag 120

cgagatcccg ccacccactt gtctggtgac gatgactcga ccatgttctg ggactacctg 180

tcgcagaacc ccgagtcgat ccaccaagtc atgatcctca tgggtgatcg aggtatcccc 240

aagggctggc gattcatgca tggctactac ggccacaccc tcaagattgt caatgacaag 300

ggcgaatggg tctacgccca gttccacctc atctctgatc agggcaccca gaacttcacg 360

ggtgacgagg ctgctcagca atccaacgat tacggacaga aggatctgta cgaagccatc 420

gagaagggag acttcccctc gtggacgatg aaggttcaga tcatgaccga gaagcaagcc 480

gaggaggcat gggagcaaaa gaggatcaac gtctttgatt tgacccacgt ctggcctcat 540

ggtgattacc cacttcgaac agtcggtaaa ttcaccctta acgagaatgc caagaactac 600

ttcgccgagg tggaacaagt cgcattcaac ccgtctcaca tgattcccgg tgtcgagccg 660

tccaacgacc cagtgttgca gtcgcgactg ttctcttacc ccgatgctca ccgacaccga 720

atcggagcca actatcagca actgcccgtt aaccagaatg tgtgcccctt cgccttgggc 780

aacttccagc gagacggcca gatggcattc tacaatcaag gtagtcgacc caactacctt 840

tcttcgattg agccaatctc attcaaggag agggcgtatg atctcaacaa ggtccacggc 900

aaattcgtcg gagaagccgt cgccttcttg tctgaaatca ggccagagga cttcaatgcc 960

ccaagggcac tgtggcagaa agtctttagc gaggaaagca agcagcgatt cgtcgacacc 1020

gtctctggtc acatgtcgac agtccgagac aaagccatca ccgctcgaat gatgactatc 1080

ttccgagaag tttcgcctga tcttggtgat cgacttgaga aggccactgg tgtcaagggc 1140

gaatccacca ttgccgggat gaagttcaac ggaacgcaca atgggtttga caaggccaac 1200

aagatcccgg ctaatgggat gaagaagggt ggagaagtca tctttgacaa tggtgcacct 1260

gctactgctg ccaggtaaat gagcggtcag gcgtacttga tatatgttgt tacgatatgt 1320

cggtctcgta gtcatgtagc caggataaat gaagcggatg tggcagtg 1368

<210> 79

<211> 1280

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 79

gagcacatac acacaccacc gcaatcatgc ctccccgcca accagcaaca cggctctttg 60

ccctaccgcc tcgcttcctc tgcccttcgc tgcccaccac gcaaacgcgc accatccgct 120

ccatcgacaa acccgcccca aaacccagcc gattcaatgc ctcactcaat ctccccgtgc 180

tgggctcctc gtccaccgcc gccttcgcgc gcaaagagca ctcgctcccc ctgcgcaccg 240

gcgcgctcgc catcaaaaag ggcatgacgg cactcttcga cccggtcaca gcgaagcgca 300

cgccctgcac cgtcctgcaa ctcgacagat gccaggtggt cagccacaag cgacgcgaca 360

tccacggcta ctgggcggtg caagtgggcg cgggcgccaa agaagcgagg aacgtcacgc 420

ggccggagag gggccacttc gccgcctaca acgtgccctt gagcaggcac ctggccgagt 480

tcagagtcaa gaacgccgag ggcctgcccc ccgttggctc ggctattacc gccgacctgt 540

tcatcgaggg ccagttcatc gatgccaaag ccgaccgcag aggcatgggt ttcgagggtg 600

gtatgaagcg ctggaacttc ggcggacagc ccgcgtcgca cggtaactcg ctcgcgcaca 660

gattgatggg ttcgtccggt ggtggacagg gcagcggtag cagagtcttg cccggcaaga 720

agatgccggg tcgcatgggt ggcgagcagg cgaccgttgc gaacctgagg gtcatgcagg 780

tggacaagga gaacggtatc gtggttgtga gtggcgctgt gcctggcccg aagaactgca 840

tggtcaagct gcaggatgcg ctcaagaagc cttggcctga tgcgacttgg ccgccgtcta 900

ttgagggcgc gacggaggtt ctgagggagg ccactgagaa ggcgcctgct gcgtaagggg 960

gtcggtcgag gtcaagaaat atcgttgcaa tttgggagat gatgctgtcc gatgcctgtc 1020

gaaaaggggt tcttgtgggg aggtctggag aatcatcgat gcaagcatta acatgagcgt 1080

gatctcacga gcaatcccag agaagcggtt acagctgctt gctcgaaatg tacactgctc 1140

aaagcttgcc ggagaagttg gccaaagtca tcactctcgg cacaggaata tactttgtaa 1200

ccatagggaa aagaggagag ggtctcgagc caggatcaaa aataggaaat gtacattata 1260

attgcatatc gtcatcatcc 1280

<210> 80

<211> 677

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 80

ggaatcgacg aacgacacct caatcgaaac caccactcgc cattgtgaat ctttccacct 60

gtcgcaatgg gtatctggga cgctttcacc gatattgtcg aggctgtgac gccatggagc 120

gtcgttgagg ccgaggctcc tgctgaggag ccccaggagg agaacgagtc caagaccgag 180

tccaaggacg agcccgagga ggaggaagag gatgaggaag aagaggagga tgaggatgat 240

gaggaggagc tcgtcgaccc caaggagact ctcgaggaag agtgcaagaa ctctcctcaa 300

tgtgcccccg ccaagcacca cttcgacgag tgtgttgagc gcgttcagca gcaggagagc 360

gagggtggtg ctaaggagga ctgtgtcgag gagttcttcc accttgccca ctgtgcgacc 420

gcttgcgccg ctcccaagct ttggtctcag ctcaagtaaa ctcacaacat tgggttatcg 480

gttactacga cgacgcaatg gctacataca cgtcgaaaag atgcctggag ccggaacgag 540

gcaatgctgc ccactacgga aggctgttcc cttgtacgaa tgctcatctg ccgggtatca 600

agtcggccag agattactct gatgtcgact ctctctgtac catacgctct tacgcctgaa 660

tagatttctt gcacttt 677

<210> 81

<211> 1019

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 81

gggagatact accgtgcgcc cgagatcatg ttgacatggc aaaaatacga tgtcgccgtc 60

gacatttgga gcacaggatg tatcttcgcc gagatgctcg agggaaagcc cctgttcccg 120

ggcaaggacc acgttaatca gttctcgatc atcacagaat tgctcggcac acctcctgac 180

gatgtcatcc agaccatcgc atctgagaac accctccgat tcgtccagtc gctgcccaag 240

cgtgagaagg tcccattcac tacgaaattc gccaatgccg acccgctttc gcttgacttg 300

ttggagaaga tgcttgtctt cgatccacgt acccgtatct cggcatcaga agggctgtcg 360

cacgagtacc ttgcgccata ccatgacccg acggatgagc ccgtcgctgc cgaggtgttt 420

gactggagtt tcaacgatgc ggatctacca gtagacacct ggaaggtcat gatgtactcc 480

gagatcctgg acttccacaa cttgggtgat atccagcaag accaggccgc cgaaggaccc 540

gtcactggcg acctagcccc accttccgct acgacttcgg catagacagc ttgcctttag 600

gggttttttt ctcgtttttc tcttctcgtc tcattacgtt cctagtcaac atgtgtccat 660

tagcatccca aattattggt ggtagaaagg agggaaggaa ttggtgcaac atgatctctc 720

ctagaaaatc gtttctcttc atctctcgtc catgatccac gctttcccaa gctttatctc 780

ccccttcccc ttcctcacgc ctcaacttct cctgtaccaa caaatcttcg ctaccgcttt 840

ctcgaccgtc gaacgaacat cacaaagaat caagaaaggt agaagaggtg tgaatagacc 900

aggaaaggca ttcttggagc gaggggggag gaggaagtaa tctggaacga aagcccatca 960

cactgttttc tttgaaccta catacacgga cagaggggaa tgcatgtgca tggtaatgt 1019

<210> 82

<211> 391

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 82

cgctaccggt cgccggcgcg ccggggttga cctcggatca ggtagggata cccgctgaac 60

ttaagcatat caataagcgg aggaaaagaa accaaccggg attgccctag taacggcgag 120

tgaagcggca agagctcaaa tttgaaagct ggccccctcg gggtccgcat tgtaatttgc 180

agaggatgct tcgggaacgg cccccatcta agtgccctgg aacgggccgt catagagggt 240

gagaatcccg tctgggatgg ggtggccgcg cccgtgtgaa gctccttcga cgagtcgagt 300

tgtttgggaa tgcagctcta attgggtggt aaatttcatc taaagctaaa tattggccgg 360

agaccgatag cgcacaagta gagtgatcga a 391

<210> 83

<211> 391

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 83

cgctaccggt cgccggcgcg ccggggttga cctcggatca ggtagggata cccgctgaac 60

ttaagcatat caataagcgg aggaaaagaa accaaccggg attgccctag taacggcgag 120

cgaagcggca agagctcaaa tttgaaagct ggccccctcg gggtccgcat tgtaatttgc 180

agaggatgct tcgggaacgg cccccatcta agtgccctgg aacgggccgt catagagggt 240

gagaatcccg tctgggatgg ggtggccgcg cccgtgtcaa gctccttcga cgagtcgagt 300

tgtttgggaa tgcagctcaa attgggtggt aaatttcatc taaagctaaa tattggccgg 360

agaccgatag cgcacaagta gagtgatcga a 391

<210> 84

<211> 124

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 84

gggtctggtg gcgatagcga gacggccaca cccgttccca tgccaaacac ggaagttaag 60

cgtctcagcg ccgaaagtag ttgggggatc tccccctgtg aggataggac gttgccaggc 120

aaaa 124

<210> 85

<211> 717

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 85

agatggagcc tgaccaagaa gagtctgaag aggaagaaga ggaagaggat gacgagatgg 60

atgaagatga ggatgagggc cagcagcagg acgccagtgg catgcagaca ccctctgggc 120

tcgccacgcc ctcaggctat gcctctacta catctacaat gcctggtggc atggagacgc 180

ctgactttat ggacttgcgc aagcagcgac agacgcgcga cgagaccgct gatcaagagg 240

accagggtgc accgcgagac ctctatacgg tcgtgcccga gcgcagagcc accgcttctg 300

gcttcctcgg ttctgaccgc gcctatgact tgtccaatgc gccacagtct tccaacatgc 360

ctgtgttggg tcaagaagac tcgcgcaaga agaaaggcgg cagatctggt gcagacgacg 420

tcgacctggc cttggatcca gctgagctcg agggcatgtc tgagcaagag cttaggcaga 480

agtacgactc gcacaggcgc tcctcgtcca gtcaaggcgc cggcggacag caggacaaag 540

aagatttctc agatttcgtc gcgcaagagg tcgcaaagaa gaggcagagg gctcagcagc 600

gcggcggcag tggacgcgac cgcgaaagct ctcggagcaa ggaaaagttc aagttttaga 660

gtgtatgttt gtattgtatg aagatcagac aaaaatgcta tgggtggcgt tgctgct 717

<210> 86

<211> 872

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 86

cgtgtagcat aaagctagaa gtaatattca cagctaactc tacagtagaa caaagttctt 60

gtttcgttct ccagatccaa gtgatagcaa ctgcttcaca aggatggaag aaccaaattg 120

ttgaaagatt cggcacctaa taggcggcac ccatgttgaa atcgtcagtc atgttgaaac 180

tccgtaaacc cgcttttggt gccctctaat ccgcaaaacc ttgaaaccct atgttgaact 240

tttggtatca tcttcgtaat cgtcataaaa cagaactccc cgtgccagag gcggcaggtt 300

gagaataccg ccccttgaat taacacatta taggaagtgg aacaaaggaa aaatgagaaa 360

tgttaatgcg cacagaatta ctgagtgtac ttctggcggt agaacttagc agcctcgacc 420

agagaggtca gctggccgat agtctgctca gtggtacggg tcttcagacc gcagtcaggg 480

ttgatccaga gctgctcagg cttgaggtac tggagcatct gctcgatacg ctccttgatc 540

tcatccacgg agggaacacg aggagagtgg atatcgtaga caccaggtcc aatgtgggcg 600

gggaaactct gatcaacgaa gacctggagg agcttggcat cggacttgct gttctcgatg 660

gacaaaacat cggtatcaag ggcagcaata gcgtggaaga agtcctggaa ttcactgtag 720

cagaagtggg agtggacctg ggtgctgtcg gtgacaccag cagtagacag cttgaaagca 780

ttgacagccc acttaacata agcatcacgg gcagcgccag tacgcagagg aagaccctca 840

cgcagggcag gctcgtcgac ttggatgacc cc 872

<210> 87

<211> 872

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 87

ggggtcatcc aagtcgacga gcctgccctg cgtgagggtc ttcctctgcg tactggcgct 60

gcccgtgatg cttatgttaa gtgggctgtc aatgctttca agctgtctac tgctggtgtc 120

accgacagca cccaggtcca ctcccacttc tgctacagtg aattccagga cttcttccac 180

gctattgctg cccttgatac cgatgttttg tccatcgaga acagcaagtc cgatgccaag 240

ctcctccagg tcttcgttga tcagagtttc cccgcccaca ttggacctgg tgtctacgat 300

atccactctc ctcgtgttcc ctccgtggat gagatcaagg agcgtatcga gcagatgctc 360

cagtacctca agcctgagca gctctggatc aaccctgact gcggtctgaa gacccgtacc 420

actgagcaga ctatcggcca gctgacctct ctggtcgagg ctgctaagtt ctaccgccag 480

aagtacactc agtaattctg tgcgcattaa catttctcat ttttcctttg ttccacttcc 540

tataatgtgt taattcaagg ggcggtattc tcaacctgcc gcctctggca cggggagttc 600

tgttttatga cgattacgaa gatgatacca aaagttcaac atagggtttc aaggttttgc 660

ggattagagg gcaccaaaag cgggtttacg gagtttcaac atgactgacg atttcaacat 720

gggtgccgcc tattaggtgc cgaatctttc aacaatttgg ttcttccatc cttgtgaagc 780

agttgctatc acttggatct ggagaacgaa acaagaactt tgttctactg tagagttagc 840

tgtgaatatt acttctagct ttatgctaca cg 872

<210> 88

<211> 362

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 88

gttgacctcg gatcaggtag ggatacccgc tgaacttaag catatcaata agcggaggaa 60

aagaaaccaa cagggattgc tctagtaacg gcgagtgaag cagcaatagc tcaaatttga 120

aatctggcgt cttcggcgtc cgagttgtaa tttgtagagg atgcttctgg gcagccaccg 180

acctaagttc cttggaacag gacgtcatag agggtgagaa tcccgtatgc ggtcggaaag 240

gcaccctaca cgtagctcct tcgacgagtc gagttgtttg ggaatgcagc tctaaatggg 300

aggtaaattt cttctaaagc taaatattgg ccagagaccg atagcgcaca agtagagtaa 360

cc 362

<210> 89

<211> 663

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 89

gaccctcact ctctttctcc ctctcttaca tagcgagctg gtctccatcc ttgttgtttg 60

atttgatctt ctttgcattt ccctatccca gtgatgaagt tatccaattc cgctcattac 120

tcgcttttcc tcctatcctc catcctcggc ttctccagcg cgtcggccaa ctctcacctc 180

agtgatgatt ctccttgtgt ggcccgctcg ccaacaagtg ggctctatta tgatctgaat 240

gctatctcat tagcaccgcc ggaatggaag aacgggaaga aagttgatca ggaagcgcga 300

gatgaaagct ggcatgccaa ggggcatgac taccccgcga acttcacaat caatgtctgc 360

gcgccggttc ttgagaatgt aaccaatgtt gtcggggtag atgcctctcg atgggcgaat 420

gtcagtgctt tctatgagca agctgggaag atatactcaa tgggagagca agcctccgag 480

cctttcttcc gcggccgcaa gctagtactc aactacacgg acggttcgcc atgtcccggt 540

gattcgaata ctgctagcgg caatagctct attcgaacca agtccactct gatgtccttc 600

ctctgcgatc gcgcggccga attccccggg ctcgagaagc ttggatccac cggatctaga 660

taa 663

<210> 90

<211> 1243

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 90

atgtccatcc gcaatgaatg gcttcaatga gaaaggcctc gacggggatg cctttggaga 60

gaagtccaat ctctccgggc taaagacatt tgacgctttc cccaaaacaa aaacatccta 120

cacaacccca acccgacgag gcggccaatg gaccgttctc atcctagcag tatgcacact 180

attcagcctc cacgaactcc gcacctggtg gcgcggcaca gaagcccacc acttcagcgt 240

ggaaaaaggc gtatcccacg atctccaatt aaacctcgat atggtcgttc acatgccctg 300

tgacactctc cgcataaaca ttcaagacgc ctccggagac cgcgttttag ctggcgaact 360

cctaacccgc gaagacacaa actgggacct ttggatgaag aagcgcaatt tcgaatccca 420

cggcgaacac gaataccaaa cgctcaatca tgaagcggct gatcgattaa gtgcgcagga 480

tgaagacgcg cacgtacacc atgtcctggg tgaagtgcgc cgtaacccgc gccgcaagtt 540

ttctaagggt ccacgtctac gctggggcga taacaaggat tcttgtcgaa tttatggaag 600

tcttgaaggg aataaagtgc aaggggattt ccatattacg gcacggggac atggatatat 660

ggaattggcg ccgcatttgg atcacgaagt cttcaatttc tcccacatga ttacagaact 720

gtccttcgga ccacactatc catcccttct aaaccctctt gacaagacca tcgccgaaag 780

cgaaacccac taccagaaat tccaatactt cctttccgtc gtcccgaccc tctactcaaa 840

gggccacaat gcacttgacc tcgtgacaac aaataaagat aactccgtcc gctacggccg 900

taacacaatc ttcacaaacc aatacgcagc cacaagccag agtaccgccc tccctgaaat 960

ccccacccta atcccgggaa tctttttcaa gtataatatc gagccgatct tgctacttgt 1020

cagcgaagag cggacgggat tcttggctct tgtcattcga gtcattaata ccgtttctgg 1080

ggtcttggtt acgggtggtt ggatctacca gatttctggg tggattgttg agatccttgg 1140

gaaaaggaaa cggcagtctg agggtgtttt gactgggaag cattattcgg attgatttgt 1200

ttctagtagt ttcgtctcaa tataagtttg attttccttt tcc 1243

<210> 91

<211> 1007

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 91

gaacggtgat agtagtagta ggctcgtcat ctatctacaa cccctctctc ctcactcccc 60

tctctcgacg ccatgttcac gcgtactctc cgaccggccg tggcggtcgc caggactcag 120

gctgtccagc agcaacaggc cggtatggcc acattgaagg aaatcgacca gcgtttgaaa 180

tccgtcaaga acattgggaa gatcaccaag tcgatgaagg tcgttgcctc gaccaagttg 240

acgcgagctg agaaggccat gcgtgaagcc aagaagtacg gtgccgccaa caacgttctg 300

ttcgagcaga ccaaggctgg tgaggaggag cccaaggagc gcaagatcct ctacctcgcc 360

atgacatccg acggtggtct gtgcggtggt atccactcca acattacgcg atacatgaag 420

aaggctgtgg ccaaggaacc cggaatgctg gctgttgtcg gtgacaagcc caaggctcag 480

ctctctcgag cgatgcccaa ggctttgacc atgtctttca acggcgtcgg caaggatgtc 540

cccactttcg tcgaggccag cgctatcgcc gatgagatta tgaaatctgc cggtgacttt 600

gacgagatcc gaatcgtctc taacaagtac ctttccgcta tcgcctacga acctcacacc 660

aacgccgtca tctccgctga ggcactccga caagccgccg gtttccagca atacgagatg 720

gaggaggatg tctccaagga cttggccgag ttcgctcttg ccaacgccat ctacactgcc 780

ctggtcgagg gacacgccgc cgagatctct gcaaggaggc aagctatgga gaacgcttcc 840

aacaacgcca acgacatgat caactctctc cagctgcagt acaaccgtgg tcgacaggct 900

gtcattacca ccgagctgat cgatatcatt accggtgcct cggctctgta agcgggtgta 960

gactagatgg acaaaacaac aaaaatggca tgcagcgaat gacattg 1007

<210> 92

<211> 1007

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 92

caatgtcatt cgctgcatgc catttttgtt gttttgtcca tctagtctac acccgcttac 60

agagccgagg caccggtaat gatatcgatc agctcggtgg taatgacagc ctgtcgacca 120

cggttgtact gcagctggag agagttgatc atgtcgttgg cgttgttgga agcgttctcc 180

atagcttgcc tccttgcaga gatctcggcg gcgtgtccct cgaccagggc agtgtagatg 240

gcgttggcaa gagcgaactc ggccaagtcc ttggagacat cctcctccat ctcgtattgc 300

tggaaaccgg cggcttgtcg gagtgcctca gcggagatga cggcgttggt gtgaggttcg 360

taggcgatag cggaaaggta cttgttagag acgattcgga tctcgtcaaa gtcaccggca 420

gatttcataa tctcatcggc gatagcgctg gcctcgacga aagtggggac atccttgccg 480

acgccgttga aagacatggt caaagccttg ggcatcgctc gagagagctg agccttgggc 540

ttgtcaccga caacagccag cattccgggt tccttggcca cagccttctt catgtatcgc 600

gtaatgttgg agtggatacc accgcacaga ccaccgtcgg atgtcatggc gaggtagagg 660

atcttgcgct ccttgggctc ctcctcacca gccttggtct gctcgaacag aacgttgttg 720

gcggcaccgt acttcttggc ttcacgcatg gccttctcag ctcgcgtcaa cttggtcgag 780

gcaacgacct tcatcgactt ggtgatcttc ccaatgttct tgacggattt caaacgctgg 840

tcgatttcct tcaatgtggc cataccggcc tgttgctgct ggacagcctg agtcctggcg 900

accgccacgg ccggtcggag agtacgcgtg aacatggcgt cgagagaggg gagtgaggag 960

agaggggttg tagatagatg acgagcctac tactactatc accgttc 1007

<210> 93

<211> 1139

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 93

acctcacttt gtgcgaatta tcctcctaca gcatcagctc tcttcagaaa gaggctaaat 60

ctatagaccg tccggaacag gttgtcaaca cgcgcgataa gagaagaagg gaatctactg 120

gtagacaaca gatcgatcgc tcttcagcaa acgcaagatg gagaaccttc ttcgtcagat 180

gcaaggggga ggtggtagga tgggtgcacg gccaggccct ggaggcgaaa ctatcctcgc 240

cgacaacggt gaaacagtcc atatttcatc tcttgctcta ttgaagatgc tcaagcatgg 300

acgagcgggt gtgcctatgg aagtcatggg tctcatgctt ggcgaatttg ttgatgacta 360

cactatctcc tgtgtcgacg tttttgcaat gcctcaatcc ggtacgacag tgacggtcga 420

atcagtggat cacgtctttc aaaccaagat gttggatatg ttaaaacaga cgggccgacc 480

cgagatggtc gtcggttggt accactcgca ccccggtttt ggttgttggc tgtccagtgt 540

cgatgtcaac actcagcagt ctttcgaaca gctacatccg cgagcagtag ccgttgtcat 600

cgaccctatc cagtctgttc gtggtaaagt cgtcatcgac gctttccgat ccatcaaccc 660

tcaatcactt gtcgctggac aagagtcgag gcaaacaacg agtaacattg gtcatctgaa 720

caaaccgtcc attcaggctc tcatacacgg tctgaatagg cattactaca gtctggccat 780

cgattacagg aaaacagaag gggagcaggg tatgttgttg aacctgcaca agcggggatg 840

gacagagggt ttgaagatgc gtgatcactc agagatgaag gagggtaatg agaaggcaat 900

caaggaaatg ctctctcttg cctcggccta cacgaaatct gttcaggaag agacgacaat 960

gacggccgaa cagcttaaaa cccgtcacgt aggaaagctt gatccaaaac gtcatttggg 1020

cgaggcggct gagaaagcga tgggtgatca agtgacgcag agtctggcca tgggtgtcct 1080

ggctgagctg tagacgtaga agagggaaga aaggaaacga catgcattgt acatatcgc 1139

<210> 94

<211> 526

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 94

aacccacccc gccattctca attcttcgtc cgtgttcttc tcgagaagct acacttcgca 60

aaaatgggtg ccattccgga atatgatccc gaggagcccc tcgagaccaa gcccttcaag 120

ttcgtgactg ctggttacga cgctcgtttc ccccagcaga accagaccaa gcactgctgg 180

caaaactacg tcgactacta caagtgtgtc gaggccaagg gtgaagactt ccgcccctgc 240

aagcagttct accacgcttt ccgctccctc tgccccaagg cctggactga ccgctgggac 300

acccagcgcg agggtggtaa cttccctgct atccttaaca aatagataac caatggctgc 360

tttgtgttgg tgaattgggt tatagcagat tctgtattga caaactttcc aatgtactct 420

acctggtcat gcggggatac atttcttttc tgtttggatg taattttccc actctgatga 480

agaaagtgtg tctataaact cgctgttttg aaactaaacg tcttcc 526

<210> 95

<211> 839

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 95

gggccattgc tcgaggagct cgatgtcgag gcgtacgcca agaagtaccg ttacctgaga 60

ttcatgtgcc aggagacgct ggaccatctc gcctttctca aggacaaagt gaaggatgtc 120

gaagggttct gggcatccac cttgttgaag caccgcgatc tcaggggcta catcacttca 180

cgatccgaca aggacgcatt gaagtacttg actcacattg agctcgttca ggatcccaag 240

gatccccgtc cgttcgctct caaattctac ttcaaggaga acccatactt ctccgacttg 300

gtcttggaga agaagtacga tatgtccgag ggttccgaac ccgcacctgc cgatggtagc 360

attacggagg gaatgcgcaa tttcaaagaa gacgagctgg tcaccaaggc taccacgatc 420

aactggaagt cggacgacaa gaatctagtc gccaagcagc ccagatccaa aattcccgac 480

aatgacgacg atgaagattt cgacggggac gtcggatcgt tcttcaacta ctttacagat 540

gacacagata ttttccagat tggggccctc ctgcagtcgg agctactgcc tgatgccatc 600

gactactttg ttggccgagg cgagcaggtg gactctgaag gagaggagct agacgagctg 660

gaagaggatg atgaagacga cgatgaggat gatgagggca gtatcgacct cgaagacgag 720

gaggagcagc cgagtaaaaa gaagcccaag agggcctaag aaacatttga tccgtcaaca 780

tgtacggacg aggtaatcgt gttcgaatgt taatgatcat gcatatgcta gtaaattcg 839

<210> 96

<211> 804

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 96

ggatctttcc tcacccctca acacactcac acaccattcg gacgcgctat gcacaatgcc 60

ttgacggttt cgaggctcaa cgacaagttc caagagccgc tcgttgttct tgtggagctt 120

ttgcgtgctc gtgttctgca cgaccgcaac ttctcgaaca gacaattctc aggtggtcct 180

tcattcggga cagacaatca gaagagaagc atgcttttga tcttccgtac tctgtccatt 240

attccgctcc aattcaaggc cgagcactgg tcaggaccat tgtcaagaga gctgcttgtc 300

ttcaactcat tccacaagac actgtcaaga tccctgagaa cgctggtcga atcaatcact 360

atgaacgcct tcctcaagaa caatgcgaga agagcacgtg acgactatct tgacattgca 420

ctttcactac cattccaaaa cgataccaac actggattcg gtatcttctt taagatttac 480

ttggatgcat tgactacatt tgcagaaggc aacatcactg aagagaacaa agacagcgag 540

tctgtgaaag aggccaagca gtcagcaatg gagatcctag gtgacgccat accgaacgtg 600

aaggacccag aggccgagct tttgcggggt ttcagattct gggatgctgt gctcgtgtgc 660

gtccgtacac tcaaagcaga cagggcaatc gatctcaagc tagctgagtc tttcgaggcg 720

gcaaacagct accttaatat gatgagacca aattgatacg gcgttttgta gcaatcttga 780

gctttatgca atctacttct gtcg 804

<210> 97

<211> 848

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 97

ggatctttcc tcacccctca acacactcac acaccattcg gacgcgctat gcacaatgcc 60

ttgacggttt cgaggctcaa cgacaagttc caagagccgc tcgttgttct tgtggagctt 120

ttgcgtgctc gtgttctgca cgaccgcaac ttctcgaaca gacaattctc aggtggtcct 180

tcattcggga cagacaatca gaagagaagc atgcttttga tcttccgtac tctgtccatt 240

attccgctcc aattcaaggc cgagcactgg tcaggaccat tgtcaagaga gctgcttgtc 300

ttcaactcat tccacaagac actgtcaaga tccctgagaa cgctggtcga atcaatcact 360

atgaacgcct tcctcaagaa caatgcgaga agagcacgtg acgactatct tgacattgca 420

ctttcactac cattccaaaa cgataccaac actggattcg gtatcttctt taaggttggt 480

ggccttgcag gagtctgaca tagcgctgac gcagacagat ttacttggat gcattgacta 540

catttgcaga aggcaacatc actgaagaga acaaagacag cgagtctgtg aaagaggcca 600

agcagtcagc aatggagatc ctaggtgacg ccataccgaa cgtgaaggac ccagaggccg 660

agcttttgcg gggtttcaga ttctgggatg ctgtgctcgt gtgcgtccgt acactcaaag 720

cagacagggc aatcgatctc aagctagctg agtctttcga ggcggcaaac agctacctta 780

atatgatgag accaaattga tacggcgttt tgtagcaatc ttgagcttta tgcaatctac 840

ttctgtcg 848

<210> 98

<211> 628

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 98

ggggcatcta cctcgacggc aacaacgacc tggtcactat gaagggtaac tacatctacc 60

acaccagcgg ccgctctcct aaggttcagg gtaacacctt gctgcacgct gtcaacaact 120

actggcacga caactccggc cacgccttcg agatcggtga gggtggttac gttctggccg 180

agggtaacgt cttccaggat gttactaccc ccgttgagga ccccgttgac ggccagctcc 240

tcacttcccc tgaccccagc accaacgctc agtgctcgtc ataccttggc cgggcctgcg 300

aaatcaacgg cttcggtaac tctggtacct tcaaccaggc tgacactagc ctgctgtcta 360

aatttaaggg tcagaacatt gcttctgctg atgcttactc taaggttgcc tcgagcgttg 420

ccagcaacgc cggtcaggga cacctgtaaa atggaaagag gaggttcaga gcttaatttg 480

ctcatgtcgg acgacatagc cctagcggct tgctggtgaa tttggcataa tagcgtttct 540

cttctcatac ctactttatt actccgtttg gatccttatt aggtaaatat tagcccattg 600

tatggttcaa ttcgattgac tttgaggc 628

<210> 99

<211> 804

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 99

agttggaaat ctgatcaatt actctccatc ttctcgttct actatctaat cctctcttcc 60

ttccaaaaca tatcatcatg tctgctcaac ctctccgcat tgtcatggcc tgtgacgagg 120

ctggtgttcc ttacaaggat gccatcaagg ccgttctcga gaagagcccc ctcgtcgcct 180

ccgtctctga cgtcggtgtc aacgatgcct ccgataagac cgcctacccc caccccgccg 240

tcgagggtgc tcaacagatc aaggccggta aggctgaccg tggcctcttc atctgcggta 300

ctggtctagg tgtcgctatc gccgccaaca aggttcccgg tattcgtgcc gttactgccc 360

acgacccttt ctccgtcgag cgttccattc tgagcaacga tgctcaggtc ctctgcatgg 420

gtcaacgtgt cattggcgtc gaacttgcga agaagcttgc cctcgattgg ctcaactacc 480

gtttcgatcc taagagtgcc tctgccgcga aggtccaggc tatctccgac tacgagacca 540

agttcgctgg ctcttcttaa atgcattatc ttgcatgaat gacggtcttc gtacatactt 600

tgccacatat gggttctaat tgcactgcgt ctgcagtctc gatatgaaac cattggattg 660

cgatggatgt cccttttcca tttgcaactt tttatatact ttcttttcta ccaagcgctt 720

catgatacca cgattcgatt accgagttct gctgtttgct ttggtcggta gatctagata 780

cacaatgcag tatattcgag tttc 804

<210> 100

<211> 782

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 100

acttctcctt ttctgttagc tttgactcta ctatcctgct cctcctctaa atccgtggaa 60

tccaattttt tcacaataac ttcgctacca taatgtccgt cactaccact tcctccgccg 120

ccgcagcctc ctgcactccc tcttggcaga ttcctgtcga cgatgttgcc tgtgccggtc 180

agatcagcgg taatatcacc aaggttttcg atacctgctg taagggaaac agccctgtca 240

agtacaacga cgactgcaac atctactgtc ttgcccaagg acaaaccaag caagagttga 300

ccgactgttt gaccgagaag agcggaaaca accagatctt ctgtggtcat ggcaagcaga 360

atgccactgc tacagctgaa gccaccacca ccaaggagac tggcacatcg accggcactt 420

caacctcttc cactggcact tctaccgaga ccaacgctgc cgtgctcaac caacccatct 480

ccaagaccgg tcttggactc gtcgccatgc tcttctgctc tgccctcgtt ggtgttgtcg 540

cctaagttat gactccaaaa cgaacacatt actgcggtat ggatacggca attatgacaa 600

ccagaggacc gcagggacgg agaatggtaa ttgatgaacc cggaaaagat acgtggtgca 660

tggacataaa tgtttgattt actcttactg tctgcttcaa ctttccgaga ggaatattgt 720

ttcttctgta ccaatagcga tagcattaac agcatcttaa ttctaatttt gcatatcact 780

tc 782

<210> 101

<211> 693

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 101

gccggggtcg atcgaggtgt catcaccaag gacgagaagg acagcagtat caatagacta 60

ctcgtcactg gttacggtct ggccgaggtc atgggtacag atggtgtgaa cggcatcaag 120

acgcgaacca atcacgtcat ggagacgtgc gaggttctgg gcatcgaagc cgctcgacag 180

accatctaca acgagattca gcataccatg acatcgcacg gaatgtcaat cgatcctcga 240

cacgttatgc tgctcggaga cgtcatgact tacaagggcg aggtgctcgg tatcactcga 300

ttcggtgtgc aaaagatgaa ggactcggtt ctcatgttgg ccagtttcga gaagaccact 360

gatcatctgt tcgatgcctc gctgttttcg aaaaaggatg aaatccaagg cgtctccgag 420

tgtatcatta tgggcacacc cgcgccaggt tgtggcacct cacttgcatc gatcgtcaca 480

cctgcccctc tcctcccacg caaaaagcct ttgctgtttg aaacagcgtt caaagctggt 540

caggatcgat tgagctatca cgaaaacaat ggcggcatgg aggtggacat gtgaacccgg 600

tccctcatac atcttcttct gattgtctgt accatacata catcgcattg cttcttttca 660

catacgacac gacatgcatc tgacatctac gac 693

<210> 102

<211> 776

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 102

gcaggatcag gaggagcata ttccttctct ctgaccacct tctcgccttc tggaaagctt 60

gtccagatcg aacatgcatt ggcagcagta gcgggtggaa caacatcact gggtatcaaa 120

gctaccaacg gtgttgtcct tgcgactgag aagaagtcac cgtcactcct gctcgatacg 180

tctgttctcg aaaaggtagc tcctatatgt cccaacattg gtttcgtcta ctcgggtatg 240

ggacccgatt tccgagtcct ggtcgccaaa gctaggaaga tcgcccaagc gtactataaa 300

gtgtatggcg agtacccacc tacaaaggtt ctagtgcagg aggtggcggg cgtgatgcaa 360

aaggctacgc aatctggtgg tgtgcgacca tatggtatct ccctcttgat cgctggttgg 420

gattcgcacc gaggtcagag cctgtaccaa gtggatccgt caggtagcta ctgggcgtgg 480

aaggcaagcg cgatcggcaa gaacatggtc aacggaaaga cattccttga gaagcgatac 540

aatgacgacc tgtcactcga agatgccatt cacacggccc ttctcacgct gaaagaaggt 600

ttcgagggac agatgactga gaacacgatc gagatcggtg tagtgacggt accgacggcc 660

gagcagatgc aggagaagcc aggagagagg ctacctccca cgttcaggaa gttgacggag 720

caggaagtga gggactatct cgccttgtag acgatgcaga cagaacatga ccatcc 776

<210> 103

<211> 1191

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 103

aagtctacga ctcctccagc caatttactt tgatccaaaa tgttgaccag cgccttttct 60

acatctgctt ccaagatgct cggcaagaga gcagtctcgt cttccagcgc cttgaacgga 120

aaggttgccg tcctcggtgc tgctggcggt attggccagc ccctctcctt gctggtcaag 180

cagaaccctg ctgtctccag cctctccctt tacgatgttc gcggctcccc tggtgttgct 240

gctgacatta gccacatcaa cacccctgct gtcaccgagg gcttcctccc cgacaacgat 300

ggcctcaagc aagccctcga gggtgctgag gtggtcctca ttcctgctgg tgttcctcgc 360

aagcccggca tgacccgtga cgaccttttc aacaccaacg cttccatcgt caagatgctt 420

gctgaggctt ctgccaagta ctgccccaag gctatgatgc tcatcattgc caaccccgtc 480

aactccaccg tgccgatcgt cgctgagacc ttcaagcgtg ctggtgtcta cgaccctgcc 540

cgtctcttcg gtgtcaccac cctcgacgtt gtccgctctt ccactttcgt ctctggcatc 600

accggtgcca agccctccga caccgtggtc caggtcatcg gtggtcactc tggcgccacc 660

atcgtgcccc tgctctccca gatccctcag ggcgacaaga ttgtcaaggc tggcggccag 720

cagtacgctg acctcgtcaa gcgcatccag tttggcggtg acgaagtcgt caaggccaag 780

gacggcactg gctccgctac cctctccatg gcttacgccg ctgccgtctt caacgacgct 840

ctcctcaagg ctatggacgg ccaaaagggt ctcgttcaac ccgcttacgt cgagagcccc 900

cacttcgcca aggagggtgc taagtacttc gcctccaacg tcgagctcgg ccccaacggt 960

gttgagaaga tcctcgacat cggcaacatg tcctctgagg agcaggagct ccttaaggag 1020

tgccttcccc agctcgccaa gaacattgct gctggtgaga agttcgtcgc tgacaactag 1080

aggatatccc acgacgttgc tccctataat aatgagagca agcgagaaca agagaaataa 1140

agacatagca aattgaatag ggcttccaac tgcaccaaaa agcagtgatg c 1191

<210> 104

<211> 1191

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 104

gcatcactgc tttttggtgc agttggaagc cctattcaat ttgctatgtc tttatttctc 60

ttgttctcgc ttgctctcat tattataggg agcaacgtcg tgggatatcc tctagttgtc 120

agcgacgaac ttctcaccag cagcaatgtt cttggcgagc tggggaaggc actccttaag 180

gagctcctgc tcctcagagg acatgttgcc gatgtcgagg atcttctcaa caccgttggg 240

gccgagctcg acgttggagg cgaagtactt agcaccctcc ttggcgaagt gggggctctc 300

gacgtaagcg ggttgaacga gacccttttg gccgtccata gccttgagga gagcgtcgtt 360

gaagacggca gcggcgtaag ccatggagag ggtagcggag ccagtgccgt ccttggcctt 420

gacgacttcg tcaccgccaa actggatgcg cttgacgagg tcagcgtact gctggccgcc 480

agccttgaca atcttgtcgc cctgagggat ctgggagagc aggggcacga tggtggcgcc 540

agagtgacca ccgatgacct ggaccacggt gtcggagggc ttggcaccgg tgatgccaga 600

gacgaaagtg gaagagcgga caacgtcgag ggtggtgaca ccgaagagac gggcagggtc 660

gtagacacca gcacgcttga aggtctcagc gacgatcggc acggtggagt tgacggggtt 720

ggcaatgatg agcatcatag ccttggggca gtacttggca gaagcctcag caagcatctt 780

gacgatggaa gcgttggtgt tgaaaaggtc gtcacgggtc atgccgggct tgcgaggaac 840

accagcagga atgaggacca cctcagcacc ctcgagggct tgcttgaggc catcgttgtc 900

ggggaggaag ccctcggtga cagcaggggt gttgatgtgg ctaatgtcag cagcaacacc 960

aggggagccg cgaacatcgt aaagggagag gctggagaca gcagggttct gcttgaccag 1020

caaggagagg ggctggccaa taccgccagc agcaccgagg acggcaacct ttccgttcaa 1080

ggcgctggaa gacgagactg ctctcttgcc gagcatcttg gaagcagatg tagaaaaggc 1140

gctggtcaac attttggatc aaagtaaatt ggctggagga gtcgtagact t 1191

<210> 105

<211> 1505

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 105

ggggcaagcg tgctgcttcc ggcactgcat ttcgacgagc gggagcaacg acctggacgt 60

acgtcttcga ctgcaagtct gctgcagcgt cacgatcgca atgaaccttt atcgctcaac 120

tcgcactcgc cgacatctgt ggaccacact cccactactg cgcatttcac tggtgctgaa 180

gagttgctcg cctccgacgt cggaccgacc gcgacagctg ggctacccgg tgatgcggag 240

cttgagagca agctcaagct gcttgaagag gtcaaacgtg cacgggaatc ggtacatagc 300

tcgctcgaga ggatcagagc cggcacgcct accccgtcta tcagccaggg aatgcccagc 360

ccgacaccct ctggtgcccc tggttacgct agaactccgt cgtctgtcgg cctgtcggac 420

gacgtgcgct cgagacgagg ctcaacgacc agctccaagg ttcttgacgc tatcgacaag 480

cctcgagtcg ctacccaatc cgaatgggac gagtacgttc gcaaccggca tgtcatctca 540

cctccaccca ctcagtttgc cgtattgccc acatctgctg cgatggtcga tcgtggtacc 600

agtcgacaca gccagtatgc ccttgtttcc gacggcgttg ccaaagcgct tgacaggcgg 660

gagcgaacta tttcaatgat ggagccgcaa gttgccgagg actggggacc aagagagacg 720

ctcgacagca ctccggctca tgtctcgatg ggccgtcgag ccatgtcatt ccatgagata 780

cctctggcat cgcctgtcgc tgcctctcga cctcaggacc gctcctccta ctctgccgga 840

ccacgtcagg tcatagggtc agctgctggc cacacgcagc gaccgggtat cagtcaatcg 900

agatcagccc acggccggac tatgacatac gacgagctga cggagagaca tcgtcagcgc 960

ttgtcggcat tgcaagcgcc agtcagcgcc aaaatcaggg agccgatgga catcgcgtcc 1020

gccaaagcca gctgggacaa gcaaaagcgg gtcgagcggg acgaaatgaa gaggcgagaa 1080

gccgagaagc tcgctcaggc tcacgcaaga gagcgacgag ggcccgctgt cgacaagaag 1140

gaagttctca agtcgaccga cgaatggagg cgaagcgtcc acggcggtct cgacggtttc 1200

gccgttccgc acctaccggc ccacgctcga ggttccacgc agcctggtgg atccggcgcc 1260

aagcgatctt cactctctca aaggcccagc aactacttcg ccaactaggc ataatcgaat 1320

cgcggacagt catctgtaca tagaaccgta cctgtattac caaccctgca cttccgctca 1380

cacctgttgc ctatacctcg tctaccaacg ctcattccaa tatcatagct acattcattt 1440

gcaaggacac tatcacaccg cagtcatgac tccgtatgga tattcaatgc atacctcttc 1500

cagag 1505

<210> 106

<211> 1391

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 106

aacctcggcc gagaggacaa gattatcaag aatgggatct cctgcgcctc accaccgcca 60

tcaatcctca cttgaaggag tcattgactt ttctactggt agagggcatc cattgaatcc 120

ctatcaacgc gacaaggccg agagcgtttt tactggcatt atcaaccgct tcgaggactc 180

gtcgaccgta gagaaaccat acaaccgtgc caagctggtt cgcctgacgt atgagtatgc 240

tcgctcggaa gattctcgat gcaatttctt gcaagcattc ttcggatcag taaacgttac 300

gatggatgac tctattgatt tcgacgatga agcggtagaa gaggggattc gctcgagcct 360

gaattccttc gcagatttct tggtggagaa cttcttcctt ccactcaagg cttccgccag 420

caggacgccc ccagcccccc agcccaagtt ccgagcagac gtcctgctgt ggggtctgtg 480

gaaagagtgg cctcgctcag acgcgactgc ctcatccgcg atcgacatcg ttgcgtaatc 540

tctcgcaact tcgacatgaa agaagctgag cgacgtcttg acgatagcgg atatgaccat 600

gcctcggacg atgaaggaca tttactgaaa gatcaggagc atgggtcatt cgcggaacta 660

gaagttgcgc atatacttcc tcactcattg atgactacga cagcgaactc cgagctgaac 720

aagtccaaag aaacggcatt gacaatactt aatatgttcg acagtggcat tgtccatcta 780

atcgacggtc cagacattga tcgccctcga aatgctctta ccttaagcat tgacctccat 840

cgacagtttg gcaacttcaa ggtttttttt gagcctatgc ctgagcccca tacctaccgg 900

attgattcaa ccctccgcca gccatttaga aacccgattt tccctgtaac ccgtgcactc 960

tacctcaccc ctgagcgaac tattgatccc ccgtccggtc gacttcttgc cgttcatcgc 1020

gcaatttgcc acattttaca tctcagtgct gctgggaatt acatcgacag catacttcgc 1080

gacatggatg acgggactgt acaagccaac ggctcgactc gcctggctag catagttcgt 1140

ctgaaactgg ggggttggtg ggatggcact gttgttggat agtcaaccac ttcgaccctc 1200

tccatacacc acaacggcaa ctcgagctga tgcatcaccg atctacctac gccattcgcg 1260

tggaggattg tcgcatatca ccactaggtt cgtgcgactg gatatgaaac gcggcccgta 1320

ctttggggtc gtgtatccgg tttcacatcc agcttgtcgc atcaaggatt ccaatcctaa 1380

cgacatgagc c 1391

<210> 107

<211> 665

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 107

ggacgaccga aataccgtca aaatggtcaa catcccgaag acgcgcagga cctactgcaa 60

gggcaaggaa tgcaagaagc acacccagca caaggtcacc cagtacaagg ctggcaaggc 120

ctccctcttc gcgcagggta agcgtcgtta cgaccgtaag cagtccggtt acggtggtca 180

gaccaagccc gtcttccaca agaaggccaa gaccaccaag aaggtcgtcc tcagattaga 240

atgcacttcg tgcaagacca aggcgcagct cgctctcaag cgctgcaagc acttcgagct 300

tggtggtgac aagaagacca agggtgccgc tcttgtcttc tagatgggtg cataacggtt 360

atggcgctag ggatgatgat ggagcggtct gtgcatgtag cctccttgag tacatgatcc 420

tcgagggctc ggaatcaaag cttcgtttct cctacgatcg tcccactcgc aaagacatgt 480

ctcgtcatat catggcttgc gcacaacatt cttcgagggt ccatcagaga tgcccgaccc 540

tgccgctacg ctgcgtggga tgtgactcca gcacaaccgc cttccagtat catctcttcg 600

cgtgcagaag tgaggacgat tttacgacag tccatataac aaatcggaaa tgccaacaag 660

atcaa 665

<210> 108

<211> 1327

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 108

ggcccctctt gaactttgga ctttttagca tctattttct ctacttctct ctccctcctc 60

ctatctacct tctctatcat ctccttcagc tccctacaac atgaagctca cctttaagga 120

cctgaagcag gagaagttcg taatcgaggt cgagccctcc gagactgttc gcgaagtcaa 180

gcaaaaaatt gctcaagaaa aaggcgaata tgaggcggaa cgaatgaaag ttatctactc 240

gggcaagatc cttcaggatg acaagaccgt cgaatcatac aacatccagg agaaggattt 300

cctagtctgt ctgccttcaa agggtcctaa gcccgctgcc tcgtcgtctg cctcccaggc 360

acccgccact ccggccccta gagctcctgt tgctactcct gctgctcctg cccccgctgc 420

tcctgcacct gctagttcta cgcctgctgt ccctgcgact ccctcgcctg ctggcgccca 480

gaccggtccc tctttcggtg acccatctgc attgaccatg ggttctgcgg ctgagggtgc 540

cgtcactcag atggaagcaa tgggatttgc cagaagcgat attgaccggg ccatgcgggc 600

tgcattcttc aatcctgacc gcgctgtcga ttacctcttg aacggtattc ccgccgatgt 660

tcaacaggaa caacagcagc ggcaacaaga gcaacaagcg gaccgtgctg cagaacaagc 720

tcctgtgccc agcgctgagg atgctgctgc tgccgccgct ctgggtggcg atgagggttt 780

taacatgttc gaggctgccg ctcaggctgg tgatggtcgt ggtggtggtg ctcggtctgg 840

aggtagcgag gcccttgcga acctggactt tctccgcagt aacccccatt tccagcaact 900

gagacagttg gtccagcagc agccgcacat gctcgaaccc atcctgcaac aggttgctgc 960

cggaaaccca cagatttccc agatcattgg ccaaaactct gaacagtttc tccaactgct 1020

aagtgaggag ggtgatgagg aagatgcggc cctgcctcct ggtacacaag ctatctccgt 1080

tacagaggag gagcgggacg ccattgagcg gttgtgccgt ctgggtttcc cccgggattc 1140

cgtcatccag gcctacttcg cctgcgacaa gaacgaagaa ctcgcagcaa acttcctctt 1200

cgaccagccg gacgatgatg aggagtaaat ctgatccacg atgctgtggt tcacttcttt 1260

actccatgtc ttatcccctt ccccttttgc ttctttacgt tctgatgaat accaagcatg 1320

cctgttg 1327

<210> 109

<211> 1326

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 109

ggcccctctt gaactttgga ctttttagca tctattttct ctacttctct ctccctcctc 60

ctatctacct tctctatcat ctccttcagc tccctacaac atgaagctca cctttaagga 120

cctgaagcag gagaagttcg taatcgaggt cgagccctcc gagactgttc gcgaagtcaa 180

gcaaaaattg ctcaagaaaa aggcgaatat gaggcggaac gaatgaaagt tatctactcg 240

ggcaagatcc ttcaggatga caagaccgtc gaatcataca acatccagga gaaggatttc 300

ctagtctgtc tgccttcaaa gggtcctaag cccgctgcct cgtcgtctgc ctcccaggca 360

cccgccactc cggcccctag agctcctgtt gctactcctg ctgctcctgc ccccgctgct 420

cctgcacctg ctagttctac gcctgctgtc cctgcgactc cctcgcctgc tggcgcccag 480

accggtccct ctttcggtga cccatctgca ttgaccatgg gttctgcggc tgagggtgcc 540

gtcactcaga tggaagcaat gggatttgcc agaagcgata ttgaccgggc catgcgggct 600

gcattcttca atcctgaccg cgctgtcgat tacctcttga acggtattcc cgccgatgtt 660

caacaggaac aacagcagcg gcaacaagag caacaagcgg accgtgctgc agaacaagct 720

cctgtgccca gcgctgagga tgctgctgct gccgccgctc tgggtggcga tgagggtttt 780

aacatgttcg aggctgccgc tcaggctggt gatggtcgtg gtggtggtgc tcggtctgga 840

ggtagcgagg cccttgcgaa cctggacttt ctccgcagta acccccattt ccagcaactg 900

agacagttgg tccagcagca gccgcacatg ctcgaaccca tcctgcaaca ggttgctgcc 960

ggaaacccac agatttccca gatcattggc caaaactctg aacagtttct ccaactgcta 1020

agtgaggagg gtgatgagga agatgcggcc ctgcctcctg gtacacaagc tatctccgtt 1080

acagaggagg agcgggacgc cattgagcgg ttgtgccgtc tgggtttccc ccgggattcc 1140

gtcatccagg cctacttcgc ctgcgacaag aacgaagaac tcgcagcaaa cttcctcttc 1200

gaccagccgg acgatgatga ggagtaaatc tgatccacga tgctgtggtt cacttcttta 1260

ctccatgtct tatccccttc cccttttgct tctttacgtt ctgatgaata ccaagcatgc 1320

ctgttg 1326

<210> 110

<211> 1162

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 110

gcgccggggg acatggagac tgccgacgcc aagaacaggg ctatgcgagc cgctggcttc 60

atcgttcccg acaccttcga agacctgccc gaggtcctca agaccaccta cactggtctg 120

gttcaaaagg gtgtcatcgt tcccaaggcc gagatcgacc cacccaacat ccccatggac 180

taccagtggg cttccaagtt gggtcttatc cgaaagcccg ccgccttcat ctcgaccatc 240

tcggacgagc gaggtcagga gttgatgtac gccggtatgc gaatctccga cgttttcaag 300

gaggagatcg gtatcggtgg tgtcatctcc ctcctgtggt tcaagcgacg attgccacct 360

ttcgcctgca aattcatcga gatggttctg caattgactg ccgaccacgg acccgccgtt 420

tcgggtgcca tgaacaccat catcaccgct cgagcaggca aggacctgat ctcgtccctg 480

gccgctggtc tcttgaccat cggtgaccga ttcggtggcg ctctcgatgg tgccgccgcc 540

gagttctctc gaggtctcaa ctctggtgct accccacgag aatttgtcga ctcgatgcga 600

aaggccaacc gattgattcc cggtatcgga cacaagatca agtcaaagac caaccccgat 660

ctccgagtcg ttctcgttgt cgattacgtc aagaagcact tcccgtctca caagacgctc 720

gactttgcct tggccgtcga ggacgtcacg acgcaaaagt ccaacacgct catcttgaac 780

gttgatggtg ctattgccgc ttccttctgt gatttgctta gcggttgcgg tgctttcact 840

gaggatgagg ctgccgatta cctcaagaac ggtactctta acggtctttt cgttcttggt 900

cgatcgatcg gtttcatcgg tcactacctc gaccaaaggc tcctcaagca gcctctctac 960

cgacaccccg ccgacgacat tttcatcaac atgcaagagc gagttgtctt ccagcctggg 1020

tccaactaag aggcgaccgc gactacgggt ctcggccaat ttctcccttg ggtttcctcc 1080

ttcaattaaa actactgtac ataccaccca catcatttat ctcttctttc atgactatag 1140

acgcatgcac gggatcgctc gg 1162

<210> 111

<211> 965

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 111

ggggcataca aggagggcaa gttcaccagc gaaagcatcc aaaagtcaaa gctcagattc 60

caggacatcc tcgttgagct gcccctcagg gttcacaact cccaccttct caccagcttc 120

ctgcaccagg tcccgcaggc gccgccggca aagaaccccc tcgacttccc ttcatccctt 180

gcagagcttt cgcgcgactc cgatgtcagc tccaacccct tcgcacccaa ccttgacacc 240

ctggacctca gcatcgaccc cttccagtac tggcagcgcg ccctcggccg cgagcagcag 300

aagatcaccg catggcaaca gaagcgcaag gctgagaatg ctgcacgcgc cgcgagcaag 360

cagccgcccc ttgacgagaa tgagtggcag aagctgttca agctgcccac ggagcccagc 420

aggctcgagg ctctgcttgt cggcaggcag gtcgagcagt acgcccgcca ggtcgacgga 480

ttctccgcca ccgtttccgc caagatgttt ggcgtcaggg gcaacctcct ccctaacgag 540

atcgagtaga ggacgaatat tacggagacg ggaccggcgt ttatgcatag cgaggcgttc 600

tcggctgggt ggggtagagt acatgcggca tggctacaaa aaaaaggatg atgtggttcc 660

gccatcgacg agttcagggc aacgctgcat agaatcccaa aagaagaaag gattttaacg 720

tttttgaatt tggaacttct tcgcattgga cgattgcttt cttgacgact ccgtcagttg 780

cgcgcttttt ccatggccca taccctcttt atctctaatg agggtgcgcc accgcagacc 840

caccagctac tcgaagaaaa gtcgctattt tttatttgga gttattagcg agtacaaacg 900

gaggcatgtc tagaggctga ggagtgtggt agtaagatta tagatgtctt tatgctcgat 960

atgag 965

<210> 112

<211> 965

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 112

ctcatatcga gcataaagac atctataatc ttactaccac actcctcagc ctctagacat 60

gcctccgttt gtactcgcta ataactccaa ataaaaaata gcgacttttc ttcgagtagc 120

tggtgggtct gcggtggcgc accctcatta gagataaaga gggtatgggc catggaaaaa 180

gcgcgcaact gacggagtcg tcaagaaagc aatcgtccaa tgcgaagaag ttccaaattc 240

aaaaacgtta aaatcctttc ttcttttggg attctatgca gcgttgccct gaactcgtcg 300

atggcggaac cacatcatcc ttttttttgt agccatgccg catgtactct accccaccca 360

gccgagaacg cctcgctatg cataaacgcc ggtcccgtct ccgtaatatt cgtcctctac 420

tcgatctcgt tagggaggag gttgcccctg acgccaaaca tcttggcgga aacggtggcg 480

gagaatccgt cgacctggcg ggcgtactgc tcgacctgcc tgccgacaag cagagcctcg 540

agcctgctgg gctccgtggg cagcttgaac agcttctgcc actcattctc gtcaaggggc 600

ggctgcttgc tcgcggcgcg tgcagcattc tcagccttgc gcttctgttg ccatgcggtg 660

atcttctgct gctcgcggcc gagggcgcgc tgccagtact ggaaggggtc gatgctgagg 720

tccagggtgt caaggttggg tgcgaagggg ttggagctga catcggagtc gcgcgaaagc 780

tctgcaaggg atgaagggaa gtcgaggggg ttctttgccg gcggcgcctg cgggacctgg 840

tgcaggaagc tggtgagaag gtgggagttg tgaaccctga ggggcagctc aacgaggatg 900

tcctggaatc tgagctttga cttttggatg ctttcgctgg tgaacttgcc ctccttgtat 960

gcccc 965

<210> 113

<211> 1160

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 113

gacttcggcg aaggcgatga gcaactcttt cataaggcac cttccttcga ctcttcgtgc 60

gtttcgttca tctactgcat tctctcttac acgttcattc tcttccacca tggcatccaa 120

cgggacatcc acaaatggcg ttcagcatga cgctcgcaag gtcttcttct tcgacatcga 180

caactgtctt tacccgaaat cgtatcaaat acacgacaag atggccgtgc tgatcgacaa 240

ctactttcaa aaccatctgt cgctgtccca agaagatgcg accactcttc atcagcggta 300

ctataaggac tacggcctcg ccatcgaggg gcttgttcgc caccacaaag tcgacccact 360

tgagtacaac gagaaggtcg acgatgcgtt gcctctggat gatatcatca aacccgatcc 420

gaaacttcga aaattgctgc aagacataga caccgacaag gtgaagctgt ggctattcac 480

caacgcctac gtgaaccacg ccaaaagggt gactcgcctg cttggtgtag acgatttgtt 540

cgaaggcatg actttttgcg actacgccgc ggaacgcctc ctctgcaagc ccacgacgga 600

gatgtacaac aaggctatgc aagaggcgaa cgccaccgat atcgatcagt gctactttgt 660

tgatgattca gcgctgaatg cggctgctgc tatgaaatac ggttggaaaa ctgcgcatct 720

ggtcgagcct accgcgaagc ctccgcccca gcccgtctca caacaccaga tcagcaacct 780

tgaagagctg cgcaaggtct tccctgaagt atttaagact tcatgatggc atggaaattt 840

taacgaagac acgagtgtat tttacgaaaa ctactcagga ttcccttgcc ttgtaagatg 900

cgaccatcgc tactgggttg ggattggaga tggtgcccag caacgctttt gcgacactat 960

caggtctaag gactctattg taaaacccgg gtcgatttgc atatggttaa ttcgaatctt 1020

ccatgaacac agcatttcgt gaaccaaaga gcacacacgt cgaagtgttg ggatgtcttt 1080

gagcagccag cttggatttc ttgagaggtc ggaagcaatt ctataggata gacagcataa 1140

atgcaataaa gccactattg 1160

<210> 114

<211> 982

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 114

atacaagact taccatcaac acaatggctc gcatctttat cactggcagc accgacggcc 60

tcggtcttct ttctgcgaag cttctctcgg aacaaggcca cagcgtcttc ctccatgccc 120

gcaatgccga acgagcatcc caggccaaag cagcagtgcc caaagcccaa ggtgtcatca 180

tcggcgatct ttcaaacgtc tcagacgtga agcagctcgc cgccgatgcc aacaaggctg 240

gaccttttga cgccgttgtt cacaatgctg gcctcggact caccaccaat ggccagaaga 300

ctgctgaggg cgtagcccag atttttgccg ttaacagcat ggcaccttac attctgaccg 360

ctctcatgga caagccgaag aggctcttgt acgtcagctc cggactgcac ttcggtggcg 420

accccagcct cgaggacgtc acttgggcca caagggagtt ccgaccatcg gatgcataca 480

acgatacaaa gatgcaaaac gtcatgctct cgaaagcagt cgccaaacgc tggcctgatg 540

tgcagagcgg ctctcttgac ccaggctggg tgaagactaa gctcggcggg tcggccgcgc 600

ctggcaccac cgacgctcca gcagagatga ttgctgagta cgctgccggc aaatcttgcg 660

caggcgatca aacaggtgcc tacttgactc cgcgtggcgt ggaagagccg catgatgcga 720

ctaagctggc cgagaagcag gatcgtctga tgcagattta caaggaggta tcgggtgttt 780

cgttccccca gtaaacacag cttcatggct ttgcctcgcg gagacctcac attttcaatt 840

agatctccct gccgattgca gcagaccagt actcactagg ctgtgcaggg ggcatgttga 900

tcaagaacga gccataacga catgccatgt caacggacaa tgagtgggcg aagtaacaca 960

tgaaattcat tatctaagcg cc 982

<210> 115

<211> 982

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 115

ggcgcttaga taatgaattt catgtgttac ttcgcccact cattgtccgt tgacatggca 60

tgtcgttatg gctcgttctt gatcaacatg ccccctgcac agcctagtga gtactggtct 120

gctgcaatcg gcagggagat ctaattgaaa atgtgaggtc tccgcgaggc aaagccatga 180

agctgtgttt actgggggaa cgaaacaccc gatacctcct tgtaaatctg catcagacga 240

tcctgcttct cggccagctt agtcgcatca tgcggctctt ccacgccacg cggagtcaag 300

taggcacctg tttgatcgcc tgcgcaagat ttgccggcag cgtactcagc aatcatctct 360

gctggagcgt cggtggtgcc aggcgcggcc gacccgccga gcttagtctt cacccagcct 420

gggtcaagag agccgctctg cacatcaggc cagcgtttgg cgactgcttt cgagagcatg 480

acgttttgca tctttgtatc gttgtatgca tccgatggtc ggaactccct tgtggcccaa 540

gtgacgtcct cgaggctggg gtcgccaccg aagtgcagtc cggagctgac gtacaagagc 600

ctcttcggct tgtccatgag agcggtcaga atgtaaggtg ccatgctgtt aacggcaaaa 660

atctgggcta cgccctcagc agtcttctgg ccattggtgg tgagtccgag gccagcattg 720

tgaacaacgg cgtcaaaagg tccagccttg ttggcatcgg cggcgagctg cttcacgtct 780

gagacgtttg aaagatcgcc gatgatgaca ccttgggctt tgggcactgc tgctttggcc 840

tgggatgctc gttcggcatt gcgggcatgg aggaagacgc tgtggccttg ttccgagaga 900

agcttcgcag aaagaagacc gaggccgtcg gtgctgccag tgataaagat gcgagccatt 960

gtgttgatgg taagtcttgt at 982

<210> 116

<211> 821

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 116

agcgactccg acaacaacga ccaccgggac gactcatatc cttcacaatg gccattggac 60

aatcctcgca gcagcaggcc gacggccaga atgtcgtcac ccagggcaac tctgacaagg 120

ccgccaaccc catgcgcgag ctgcgcatcc agaagctcgt cctcaacatc tccgtcggcg 180

agtctggtga cagacttact cgtgccgcca aggtgctcga gcagctgagc ggtcagaccc 240

ccgtctacag caaggcccgc tacaccgtcc gtaccttcgg tatccgccgt aacgagaaga 300

tctccgtcca cgttaccgtc cgtggcgcca aggccgagga gatcctcgag cgtggcctca 360

aggtcaagga gtacgagctc cgcaagcgca acttctctgc caccggtaat ttcggtttcg 420

gtatctccga gcacatcgac ctgggtatca agtacgaccc tgcgatcggt atctacggca 480

tggacttcta cgtcgtcatg tcccgtcccg gtgagcgtgt cgcccgccgc cgtcgcgcga 540

agacccgcgt tggtgcttct cacaaggtca acgctcccga ggtcatcaag tggtacaaga 600

accgcttcga gggcatcgtc aggtaaaaag cttgaaaggt ggtctgggat gatgaaaaat 660

tcaacttgtg gttttggcaa cggcgcaaaa gagcgaggct atttttccgt agcttgagga 720

tatatccggc ctatcggagc tttactttta cgcttgagca agatcgcaaa aatggaggcc 780

tcgtatacca agcgagcgtg ccgcataacc attgatcgct c 821

<210> 117

<211> 674

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 117

ggacacaccg gtgacgtctt gagcgtctcg ttctcggccg acaaccgaca aatcgtttct 60

gcttcccgag accgaactat caagctctgg aacactctcg gagagtgcaa gttcaacatt 120

gttgacgatg gtcactcgga gtgggtctct tgcgttcgat tctctcctaa ccccgtcatt 180

cccgtcatcg tctctgctgg ttgggacaag gtcgtcaagg tctgggaatt gtccaagtgc 240

aagctcaaga ccaaccacca cggtcacact ggttacatca acaccctcgc cgtttcgccc 300

gacggatcgc tcgccgcatc cggtggaaag gatggcatca ccatgctttg ggatttgaac 360

gatggcaaac acctctactc tctagaggct ggagacattg tcaactcgct cgtcttctct 420

cctaaccgat actggctctg tgccgccact gcttcgtcaa tcaagatctt cgacttggag 480

tccaagtcaa tcgttgacga cctcaagcca gacttctccg ccgagtactc tgacaaggct 540

caaaagccac aatgtacttc cctcgcctgg tctgccgatg gtcagaccct ctttgccggt 600

ttctccgaca acctcgtccg agtctgggtt gtcactgctt agagtcgtga ggattgtatg 660

catggataac gtgg 674

<210> 118

<211> 1183

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 118

gaagtaccgt tttctgtgca gtttttttta aacccagaac ttgcaattga gatcacgcgt 60

cgcaatggca ccctctaccc agaagcaatg gaccgttaaa aacggggagc aggactttga 120

cggcctcgtt tacggcgacg cgccagttcc gactgcgggg gactcggaag tcgttgtcaa 180

gctccatggt gcctcgctca actaccgtga cctgattatc cccaagggaa agtacccctt 240

cccgctctcg ttcccggtcg tccccggctc tgacggtgcc ggtgaagtcg tcgaggtcgg 300

atccaaggtc aagcaattca agaagggcga caaggttgtt accctcttca accagctcca 360

tcagtacggt cccgttgacg ctgctgcggc atcgtcgggc ctcggtggtg cggttgacgg 420

aaccctgcgc cagtacggtg tcttcaatga gaacggcgtc gtcagggccc cgaccaacct 480

gaacttcctt gagtcgagca cactaacctg tgcgggacta acaagctgga atgcgctgta 540

tgggctgaag ccgcttcttc ctggccagac cgtcctggtg cagggcactg gcggtgtgag 600

tatctttgct ttgcagttcg caaaagcagc gggcgcaact gtgatcgcaa caacctcatc 660

cgaagagaaa ggcaagcgcc ttaaggacct cggtgccgat cacgtcatta actacaagac 720

ccaaaccaac tggggcgaga tcgcgcgcgg tttgacgcgc gacaacatcg gggttgacca 780

catcattgag gttggaggcg ccggcaccct ggagcagagc ttcaagtgca tcaagttcga 840

gggagtcatt agtattattg gcttcttggg cggaatgaac cccagcacca tacccaatgt 900

tctgcagacc ctgagcaaca tctgcactgt gcgcggtgtg tatgttggca gcaaggcgct 960

gatgaacgac atgatcaacg ccatcgaggc gaacaatatc caccctgttg tggatggaac 1020

tgtgttcacc cttgagaaga cacgagaggc ctatgagtac atgtgggcgc agaagcactt 1080

cggaaagctg accatccaga tcgcttaatc acttgatgaa tataatgagg gatatatgcg 1140

actaggaatt atgcgctaat gaatataata accatgcaat tag 1183

<210> 119

<211> 1563

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 119

ggggccatgc tcgagcagca gtaccagatg cgaaaggagc agcaagtgca atttacacct 60

atggcatcgc cgtccagcac tccttaccac atgcatcaag atttcactgt tccgggcgac 120

tttttctccc ccctcacatc gcctgcgctc cacgctcaga atcagccaca atcgcgacag 180

caattcacgg ctcatcaaca gggctactac acgaatccca gcaccgctgc gagctcggcg 240

gctccgagtc caatcgacgc gaacggagat gtggaaatgg gtggcgacgg tgttgcgctg 300

ccagagtcag cgagccaacc gaagaagcct tcccgaagga agcctgcgac accgaggact 360

ttcgccatga acaaggtcaa gcaaagtccc atacaaaaac cgcaaaaaag gaagtctgtg 420

gcgttggcac acaaggatgc agatgctgtg gtgcaggacg cccaacggtc tggccatatc 480

gcgcccaaat ccgcaggtct ccaaatgccg cctccgtttg agagctcgga aaacgacagt 540

gtttcgccgg aagcgctgaa cgacctgcct atgggccccc cgcctagacc tggatcggtt 600

tcgcagtcgc ccgccatcgc tcctcagaat cagagcgttt ctggaccggc cgcgactccc 660

aaatctctcc tttctatgaa gggcgctcaa gatatgaatg cacctgccag tactggtatt 720

tctggccaaa tgggacaggc atccttagaa gatctcgaac ttcccgaagc tgccgaaaat 780

ccaggatcga ctgcgacaca ctcgcaagtc ttgaactcgc aagagccgac acctcgcctc 840

atgccctccc gtaaaacgcc aaaactcggc cctcttagca cgccttcatc gggcaagcct 900

acttctgctt ccaacagtcc cgctcatgcg ttgtctccca tgacagcgag tacccctgct 960

ggtctgctga aggacaagaa ggacaacaaa ggcggacgtg caaccagcaa gaagcgtggt 1020

agtgtcagta ccaccaattc agcaatggtc tctccggcac tccgaccgaa ggtcagcccg 1080

agtatcaagc ctctgctacc cgaaggcacc agcctcaact ccccgaccca tgccctcctc 1140

ctcgcctcca aatccaatta ccagaacctc ctggaaggca accacctccc cggcatctcc 1200

tacccggact ccctctcaac cggcctcacc agcaaacgca cctcgcacaa agtcgccgag 1260

caaggccgcc gcaaccgcat caacgacgcc ctcaaagaaa tgcaagccct catccccgcc 1320

tcgtccggcg cccgcgccga agagctcatg accgccgacg ccggcgacga cgacagccag 1380

gaaaccaagg agaaggaccg cgacgccgct gtcaagagca atagctccaa agccgcgacc 1440

gtcgagagtg cgaatcggta tattcgcgtg ttgaaggaga gcgacgcggc gcagaaggat 1500

gcgatcgcgc ggccgaattc cccgggctcg agaagcttgg atccaccgga tctagataac 1560

tga 1563

<210> 120

<211> 939

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 120

gacgacacaa tgagaaacat cctcctagta ttggcgtctg cagcgcttgc tgttgtggca 60

caaaagccag atctcgacgt gaaaggcacg tttggagacg cgaacccctt ctccaaggtc 120

gtcaacggcc aaagcaacaa gctctacctc acgctggaca accacagccc tgagtctctg 180

gtggtcaagt ctatcagcgg gtcatggtct gagaagacgt ccgcttcatc cggtcaagag 240

aagtttctta agaactctac cacccaagag aagctcactg tccccatccc tcccaagtcc 300

gagggcgcat tccagcctcc tacagtcttg acctaccagt tctggagcga attcaagcct 360

agagagttgc tcttgaccgt tttgggttga ctatgttgat gctaccggtc actcgtacag 420

agaaacagcc tacgaaggcc aagtgactgt cgttgaggcc ccgggatctt tctttgaccc 480

cgccttgctc tttgcctacg ccatggtgct ggctctcgtc ggcggcgccg gctaccttgc 540

ctacaacatc tactttccac ctgcccgcaa gcccagaaga agcgccaaca ccgcacctac 600

agatgctcct gctgctccgg ctgaccctga cgaatggatt cctgtccacc acaagagggc 660

caaaaagacg tctggcggcg gggccaccag tggtgaagag agcgaagcca ctgaaggcta 720

tgcaagcgag aagtctgcca gtggagccaa gaagagaggc aaaggtggca gaaaataaat 780

actgacatgt gcctcgagct gcagacgacg ctcgtcaaaa gtgtagcaag ttgaagaagc 840

ccagcacgaa gtccccagct tgactgctgc cgtttggctt aatggcacag aaagcgagtg 900

tacgtcgtac acggcttata gtctcgaatg caacaaagg 939

<210> 121

<211> 896

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 121

gccaaccacg acattgatcc ctttcacgac ttgctttcgc catgtcagat aacaacgatg 60

gaaatcacgg gggaggcgta ggcgcgtctt actactatgg cggcatcgcg attgcgctgt 120

gtcttgtgat tgtgttgacg cttgtatcaa gaatattata tcgacgacgt gtaaggaaca 180

gactcctgcg agccaacaga caagagcgca ttactttgcg agaccgggga gaagcgccag 240

gcctgccaac ctatcgggag tctcgcaatc agccctcatt accgcgatac acggccgagg 300

cagactacgc acctccaccc ggcccgcctc cttccaacag cccggacaac gaaggccacc 360

acttccactt ccatttcccc tctttacatg tgcctcaggc actgcacttg cggcctaggc 420

aggcagacga tcctgctgac cagatcccca ccgtgccccc tccgtcctac gagccgccca 480

agtatgagcc gcccagtgga gcgcctccag agcagcaaca agagcctgtg gctagcggga 540

gtagcgagca tcatcaccag cagtctgctt tgggcgaaca taccgcggcg gcaacagccg 600

ctgccacaac tccggcagag cacagtggcg agtcgacaga gcttaggagt gcgtcgcctt 660

ctcagccaca atctcaatcc caacctcaag caccagcaca accacaagag caggattacg 720

gctacgacga tgccgacttt atccatcctg aagagcgacg caggatcgag gctgcgcagc 780

gcaatgatcc gcagacatga ttcaaacatg tgttgtaaag tgtactacta tgaactcgtt 840

gaccagtata atcgaagcgt atataacggc acaaatgcaa agctgccatc atcccg 896

<210> 122

<211> 697

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 122

cattacggcc gggaagcttt cagaagctaa tcgagttctt cctatccctt tcaactttac 60

acaccatgtc tagaattggc gatccaacga acaaccctgc tacccagcaa ctgtactctg 120

ataggccctt gcatctccct ggccccggcc tcaagccatc caggcagctc actatcagct 180

cggctgttgc gttccgcgag gattcgggcc aaacacgctt caacctcatc agctctgacc 240

accgcgaggt gttgcacatt agtattcgtg caagggacaa cgttctcgtg ctcaacacca 300

aggcccccga tggcgattgg ggcaaagaag agcgacatga tctcaaaccc cttttcgata 360

ccccactgct gccttacatc accgtaatgg caacgaagaa cagctatatc ctttctgttc 420

ctggtaaacg ggagatcatc ttcaataaga ggaaagggtt catggagcct gctgtgagga 480

ttgagtatga ctatgatgag atgtctgcgt tctccgaccc ctgctacatt acagtcccat 540

cttcatctta aagctttcct agttggcttg gagttggcgg atatggtcac attggttttt 600

tcacacggca aacggtaaag aattacggct tctctctcct gtcatgttca gcggacgatg 660

tatgatgtag tgttctgttc aattgatctg gttgttg 697

<210> 123

<211> 329

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 123

ggaacgacca cgagcagttt ttaaaatgcc acaagaaatc aaggacatca agaacttact 60

cgaaatcgct cgtcgtaagg acgctcgttc cgcccgcatc aagaagacca agaccgttgg 120

tgctaagggc gagccagctc aacttaccaa gttcaagatt cgttgctctc gctacctcta 180

cactctcgtc gtctctgacg gtgagaaggc agagaagctt aagcaatcac tcccaccaac 240

cctcaacgtc gaggagattg gtaaggtttc aaagaagtag attagtgatg taatttgctg 300

ccttgattga ttgtccttgt tggtatttt 329

<210> 124

<211> 747

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 124

gtacacataa ctcttcattc ctcatcatgt ctcacacttt ctacgatggc accatcgtgg 60

tgcttcaagg cattcttgaa actttttctc atatccttca caaagccgaa gaaagcccaa 120

actctagcgc ttttcccgca gctcgtctgc acgaggacat gtatccattg accgaccaaa 180

ttcgcctagc aactcaattt tctgagtata ttctggctaa agtgaccggc cgcgagccaa 240

ggaagttcga aggcaatcca ttgaccttcg ctgaattcta tgagcgtatc gataccatgc 300

tgaagtcact caaagaagca gataaggatg tcgtcaatgc aaatgccgac aaggaggagc 360

ttactcaagt tggacctacc gcaaaaattg aattgagtaa tgctatatac gcccatcgca 420

tagccttgcc caacatttac ttccatctca acattgctta cggcattttg cggaaggagg 480

gcgtgcctct tggcaagctt gactattttg cgggcttttt cccaccgagc atggctcaag 540

gcaagtaaag aagtgatgtt ggttatgttt ccggatggag agggtgctga tctatgagaa 600

tgagttccga gtagaccatg atggtctaga tgtggacttg agctttcatt tgccaaattc 660

ttgtggaaag atagcaatga cggaacaagc gatttgtatg tacatttaat gaagtctatc 720

tatagaatta atctccgatc tatcgcg 747

<210> 125

<211> 693

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 125

aagctcttca aagctaatta tcgagttctc ctatctcttg cacttataca caccatgtct 60

agaattggcg attttgcgaa caacaaccag gctacccagc agctgttctc tgatagaccc 120

atgcagctcc ctggccccgg ccttaagccg tccaggcagc tcacggtcag ctcagctatg 180

gcgttccgct gggactctgg ccaaacccgc ttcaacctca tcagctctga ccgtcgtgaa 240

gtgctgcaca tcagcatccg cgcaaaagac gacgtccttg tgcttaacac taaggctcct 300

gatggcaatt ggggcaagga agagcgacac gagctcaaac cccttttcga caccccgatg 360

ctgccttata tcaccgtaac ggcgactaag actagctata tcctgtccgt tcctggtaat 420

caggagatca tcttcaataa gaggaaaggg ttcatggagc ctgctgtcaa gattgagtat 480

gactatgacg agaaccctgc gttctctgat ccgtgctacg tcacagttcc gcatttatct 540

taaggtctta ttggcttgga gttggcggat agtcacaccg gtttttttca cacggcaaaa 600

ggcaaagtat tacggctttt ctctcctgtc ctgtttagcg gatgtacgat gtatgttgta 660

gtagtgttct ggaatttgtg ttcaagttgt tgg 693

<210> 126

<211> 1030

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 126

gagcgacttc atcaaaaatg tcagagcaac ttcactacaa gggttcattg gccggccacg 60

gcaactgggt tactgccatc gctacctctg cagagaaccc agacatgatc ctcactgctt 120

cccgtgacaa gtctgtcatc gtctggcaac tcacccgtga cgacgctcag tacggttacc 180

caaagagaat cctcaagggc cacaaccact tcgtctctga cgtctccatc tcatacgacg 240

gtcaattcgc tttgtcctcc tcatgggaca agaccctccg tctctgggac ctcaacactg 300

gtcttaccac cagacgtttc gttggccacg aagcagacgt tctctccgtc tccttctccg 360

ccgacaacag acaaatcgtc tctggctccc gcgaccgcac catcaagctc tggaacaccc 420

ttggtgaatg caagttcgac atcaaggatg aaggccactc cgaatgggtt tcatgcgttc 480

gtttctctcc aaacccaatg aacccagtca tcgtctcagc tggttgggac aaggttgtca 540

aggtttggga actctcaaac tgcaagctca agaccaacca ctacggtcac actggctaca 600

tcaacaccgt ctctgtctcc ccagacggat cccttgctgc ctccggcggt aaggacggca 660

tcaccatgct ttgggacctc aacgagggca agcacctcta ctccctcgag gctggtgaca 720

ttgtcaacgc actcgtcttc tcaccaaacc gttactggtt gtgcgctgct actgcctcat 780

gcatcaagat cttcgacctc gagtccaagt ccatcgtcga cgagctcaag ccagactttg 840

tcgacgtcgg caagaactcc cgcgagccag aagctgtctc cctctcctgg tccgctgatg 900

gtcaaaccct cttcgctggt ttcaccgaca acgccgtccg tgtctggacc gtcgcataaa 960

actaagctgt atctaataga cagggtattg ggttttgtaa cactattgcg aggaactcat 1020

gattttaccg 1030

<210> 127

<211> 668

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 127

ggggaaggtg gtggtatcca cggaaccacc ttcaactcca tcatgaagtg tgatgttgac 60

gtccgtaagg atctctatgg caacattgtc atgtctggtg gtactactat gtaccctggt 120

attgccgacc gtatgcagaa ggaaatcacc gctcttgctc cttcgtcgat gaaggtcaag 180

atcattgctc ctcctgagcg taaatactct gtgtggattg gtggttccat cctggcttct 240

ctgtccacct tccagcagat gtggatctcg aagcaggagt acgacgagag cggcccttcg 300

atcgtccacc gcaagtgctt ctaagcttaa gcgcatggtt gatttgcttg tttgtacttc 360

ttttctggcg tatcaaaagg caggacagtg tggcatgcgg accttttctg acctgatgac 420

gagagggatc gcctaagaaa aaggaacttt attttagttg tggaatagag acggtttatt 480

tgacgctagt tctcgtccag agcatcctcg agacgatagt ctgggttcgt cttaagcgat 540

ggatggtggt gattctcttc gtattgttcc tgtacctgta ctacatattg cctacaccat 600

gtcctgttca tttcttctct gtttgcgttg cgttagacct tataaattta aatgtcgtat 660

tgctcccc 668

<210> 128

<211> 668

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 128

ggggagcaat acgacattta aatttataag gtctaacgca acgcaaacag agaagaaatg 60

aacaggacat ggtgtaggca atatgtagta caggtacagg aacaatacga agagaatcac 120

caccatccat cgcttaagac gaacccagac tatcgtctcg aggatgctct ggacgagaac 180

tagcgtcaaa taaaccgtct ctattccaca actaaaataa agttcctttt tcttaggcga 240

tccctctcgt catcaggtca gaaaaggtcc gcatgccaca ctgtcctgcc ttttgatacg 300

ccagaaaaga agtacaaaca agcaaatcaa ccatgcgctt aagcttagaa gcacttgcgg 360

tggacgatcg aagggccgct ctcgtcgtac tcctgcttcg agatccacat ctgctggaag 420

gtggacagag aagccaggat ggaaccacca atccacacag agtatttacg ctcaggagga 480

gcaatgatct tgaccttcat cgacgaagga gcaagagcgg tgatttcctt ctgcatacgg 540

tcggcaatac cagggtacat agtagtacca ccagacatga caatgttgcc atagagatcc 600

ttacggacgt caacatcaca cttcatgatg gagttgaagg tggttccgtg gataccacca 660

ccttcccc 668

<210> 129

<211> 1018

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 129

gactttctca tttcagaatt attttctata ctctgacaag agcaagcaat accaaacatc 60

ttccacatcg aagctttaac cattttgccc ttaacatttg aacaagacga aatggccttc 120

ttcccacact acaccactaa tctgtcgcct ctgctctact tgttggacga cgactatgct 180

gtctaccgct caacttgtcc aaagtccaac taccaccaca agcaacacca cagccgccgt 240

cagccttcgc cagttcgtta ctttagtccg aattttgata tgcgagaggg gaatgactcc 300

tactaccttg acggagagct ccctggtgtc aaccagaatg atgtcgatat tgaattctct 360

gaccctcaga cactggtgat caagggtcga gtggagcgga attacaacaa tctcgacggc 420

atgaacgagg aaaaccagca agatgaagaa caattctctg aaactctctc tagcaagtcg 480

taccaaccca ctgtcgagga cgaggacgag gcgaaccatt caccacccgt ggcgacacca 540

acctactctg agaagtctgt tactgagaaa actcagaagc ctgcgtacaa ataccgaaat 600

tctgaacgtg ctattggcga attccaccga gccttcaatc tccctacaag agtcgatcaa 660

gatgcggtca gggctacatt gaggaatgga atcctctcgc tggagctccc gaaggagccg 720

gcaccgaaga tgaagaagat tcggattgaa tagaggattt cgaataaaat ttttgatttg 780

atgagtagtt ggtgtttatt gttatgtcta attatatggg gctatgtcat gattgggaaa 840

tgggacaccg catttgtttc ctttttcccc atttcttcag acgccatcta tattgcatgt 900

atgttgcatg aactatggtt tttgctagga gcggttgctt ctgctttgca ttttcatgaa 960

ctattttctt tttattaaat taataactag catatcaatt aatgatctgt catatggc 1018

<210> 130

<211> 686

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 130

aagctttcag aagctaatcg agttcttcct atccctttca actttacaca ccatgtctag 60

aattggcgat ccaacgaaca accctgctac ccagcaactg tactctgata ggcccttgca 120

tctccctggc cccggcctca agccatccag gcagctcact atcagctcgg ctgttgcgtt 180

ccgcgaggat tcgggccaaa cacgcttcaa cctcatcagc tctgaccacc gcgaggtgtt 240

gcacattagt attcgtgcaa gggacaacgt tctcgtgctc aacaccaagg cccccgatgg 300

cgattggggc aaagaagagc gacatgatct caaacccctt ttcgataccc cactgctgcc 360

ttacatcacc gtaatggcaa cgaagaacag ctatatcctt tctgttcctg gtaaacggga 420

gatcatcttc aataagagga aagggttcat ggagcctgct gtgaggattg agtatgacta 480

tgatgagatg tctgcgttct ccgacccctg ctacattaca gtcccatctt catcttaaag 540

ctttcctagt tggcttggag ttggcggata tggtcacatt ggttttttca cacggcaaac 600

ggtaaagaat tacggcttct ctctcctgtc atgttcagcg gacgatgtat gatgtagtgt 660

tctgttcaat tgatctggtt gttgac 686

<210> 131

<211> 698

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 131

gggcatataa ccacaggtca ttcgcatccg tcgcagaact tcttacatct gagctttcct 60

gtccgttaga tataggcaaa atgaaggcct actggtacga taaccaaccg ggcgaccagc 120

gcttgcctca cgactccggc cgccccgtca ccgagtccta cctcgagtcc atcggcgtct 180

tctaccgcca ctgcccaaca attgaccttg tcgactccct ggccgccgag cgcggctaca 240

agaaccgcga cgaggtctgc gtctcgccgc agactatggg cgatgtctac gaggagaagg 300

tgaagacgtt ctttagtgaa catttgcacg aggacgagga gattcggtac attcgagatg 360

gggaggggta ctttgatgtg cgtgggcagg aggatgagtg ggtacggatt cggttgagta 420

aggatgatct gatcattctt ccggctggga tctaccatcg gtttacgaca gatgataaga 480

actacgtcaa ggctatgcgt ctcttccagg aggagcccaa gtggacgccc ttgaaccgtg 540

gccctgaggt tgatgtcaac cctcaccgga agacatacct ggaaaccgtc cccagccctg 600

ctgtggctgc gaactaagtg agcatcgaat gctcttgttg aacaatctat ttgcacatct 660

ttagccttta tacacctcaa tgcatcaatg gatttagg 698

<210> 132

<211> 884

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 132

gtggctgccg gctacacccc cgaggccctc gagatcctct ccaagaagaa gggcggcaag 60

tacctcgtcc tcgagatgga cgagacctac aacccccccg ccgaggagac acgtactctc 120

tacggtgtcc agctcaccca ggcccgcaac gatgctgtca tctcccccca gaagaccttc 180

aataccatca ttacccccaa gaacaccgag tccctccccg agtccgccct ccgcgacctc 240

accgtcgcca ccctcgccct gaaatacaca cagtccaact ccgtctgcta cgcgctcaac 300

ggacaggtcg tcggcctcgg tgccggccag caaagtcgta tccactgcac tcgtcttgcc 360

ggcgacaaga ccgacaactg gtggatgcgc ttccacgagc gcgtgctcaa catcaagtgg 420

aagcagggca ccaagcgtgc tgacaagagc aacgccatcg acctgctctg ctcgggccag 480

acgccccgca atgacgctga gaaggtcgag tacgagcgtg tgttcgcgga ggttcctgct 540

ccgttcaccc aggaggagcg tgatgcttgg ctctcgcagt tgaccaacgt tgctatttct 600

tcggatgctt tcgtatgtct ctcccctctg ttagagcatt ctaagttcta agatcatgct 660

aattggtgaa atagttcccc ttcatcgaca acgtcttccg agccgcccgc tccggcgtca 720

agtacatcgc tgcacccagc ggttcgcaga acgacggccc tgtcttcgag actgccgaga 780

agcttggtat ctcgttcgtt gagcagggta ctcgtctgtt ccaccactaa cttgcttttc 840

cggtggcgtg gtattatggt ataaaaagaa aaagggtttg gggg 884

<210> 133

<211> 822

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 133

gtggctgccg gctacacccc cgaggccctc gagatcctct ccaagaagaa gggcggcaag 60

tacctcgtcc tcgagatgga cgagacctac aacccccccg ccgaggagac acgtactctc 120

tacggtgtcc agctcaccca ggcccgcaac gatgctgtca tctcccccca gaagaccttc 180

aataccatca ttacccccaa gaacaccgag tccctccccg agtccgccct ccgcgacctc 240

accgtcgcca ccctcgccct gaaatacaca cagtccaact ccgtctgcta cgcgctcaac 300

ggacaggtcg tcggcctcgg tgccggccag caaagtcgta tccactgcac tcgtcttgcc 360

ggcgacaaga ccgacaactg gtggatgcgc ttccacgagc gcgtgctcaa catcaagtgg 420

aagcagggca ccaagcgtgc tgacaagagc aacgccatcg acctgctctg ctcgggccag 480

acgccccgca atgacgctga gaaggtcgag tacgagcgtg tgttcgcgga ggttcctgct 540

ccgttcaccc aggaggagcg tgatgcttgg ctctcgcagt tgaccaacgt tgctatttct 600

tcggatgctt tcttcccctt catcgacaac gtcttccgag ccgcccgctc cggcgtcaag 660

tacatcgctg cacccagcgg ttcgcagaac gacggccctg tcttcgagac tgccgagaag 720

cttggtatct cgttcgttga gcagggtact cgtctgttcc accactaact tgcttttccg 780

gtggcgtggt attatggtat aaaaagaaaa agggtttggg gg 822

<210> 134

<211> 996

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 134

tgaagcagtg gtatcaacgc aagcagtggt atcaacgcag aatgtgcgat cgctctagaa 60

tcggtcccaa gggttgggaa gcagtggtat caacgcaagc agtggtatca acgcaagcag 120

tggtatcaac gcaagcagtg gtatcaacgc aagcagtggt atcaacgcaa gcagtggtat 180

caacgcagag tgcgcagccc ggtgctatct ctgctcctgt ggcagctggt aaggacgttg 240

agctgcagtg gaccgaatgg ccggaaagtc atcatggccc tgtcattact tacctggcca 300

actgcaacgg tgactgctct gaggtcgaca aatcctctct ggagtttttc aagatcgatc 360

agaagggtct catcgatgac agcaatgtcc ctggcacatg ggctaccgac aaactaatct 420

ccaacaacaa cagctacacc gtcaccatcc ccagcgacat tgctgccggt aactacgtcc 480

tccgccatga aatcattgct ctgcactccg ctggcaacga ggatggtgcc cagaactacc 540

cccagtgtct caacctcaag gttactggtg gtggcaacgc ttctccctca ggtactcttg 600

gtaccaagct ctacaacgag gacgactcgg gtatccttgt cagtatctac cagcagcttg 660

actcctacga catccccggc cctgctctgt actctggcgc ttcctcgtcc tccaactctg 720

gttcttcttc cagcgttgct tcggccactg cttctgccac ttctgccgct gcttcctctc 780

cctcgtcctc tcaggcttcc ggtacccccg cttcccaggt caaggctcag accgctagct 840

ctactcctag cgcttcgtcc ggtgccactt ccggcagtct gtccgactac ttcagctctc 900

tgagcgctga ggagttcctc aacgttatca gcgagactct gtcttggttg gtcactgaca 960

agattcacgc tcgtgacttg tcgaccgcat aaatgg 996

<210> 135

<211> 823

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 135

gtggtatcaa cgcagagtgc gcagcccggt gctatctctg ctcctgtggc agctggtaag 60

gacgttgagc tgcagtggac cgaatggccg gaaagtcatc atggccctgt cattacttac 120

ctggccaact gcaacggtga ctgctctgag gtcgacaaat cctctctgga gtttttcaag 180

atcgatcaga agggtctcat cgatgacagc aatgtccctg gcacatgggc taccgacaaa 240

ctaatctcca acaacaacag ctacaccgtc accatcccca gcgacattgc tgccggtaac 300

tacgtcctcc gccatgaaat cattgctctg cactccgctg gcaacgagga tggtgcccag 360

aactaccccc agtgtctcaa cctcaaggtt actggtggtg gcaacgcttc tccctcaggt 420

actcttggta ccaagctcta caacgaggac gactcgggta tccttgtcag tatctaccag 480

cagcttgact cctacgacat ccccggccct gctctgtact ctggcgcttc ctcgtcctcc 540

aactctggtt cttcttccag cgttgcttcg gccactgctt ctgccacttc tgccgctgct 600

tcctctccct cgtcctctca ggcttccggt acccccgctt cccaggtcaa ggctcagacc 660

gctagctcta ctcctagcgc ttcgtccggt gccacttccg gcagtctgtc cgactacttc 720

agctctctga gcgctgagga gttcctcaac gttatcagcg agactctgtc ttggttggtc 780

actgacaaga ttcacgctcg tgacttgtcg accgcataaa tgg 823

<210> 136

<211> 1000

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 136

gacggtgaag ttggaataga ataaaatgtt gagcatgttt accagagtgg ccagaggaca 60

ggccaaggtg tttacccgca acgcatccac agcatcatcc aaaccaacga atcaatcatc 120

caacaaggct gccactatcg cagcttcaat ttcaggtgtt accgccgcgt tatacgccca 180

ccaatacggc ctcattgaca gcgtcttcgc tagtggctta gaagagggtt tgcacgctcc 240

tcatttccct tggtcacaca atggctggtt ggacagcttt gaccacaact ccattagacg 300

cggttaccaa gtttaccgtg aggtgtgcag ctcgtgtcac tctttggaca gaatagcgtg 360

gagaaacctt gtcgctgtgt cacacacttc agatgaagcc agagcgatgg ctgaagagca 420

agagtacact gatggtccaa atgaccaagg agagtctttc caaagacctg gtaaattggc 480

tgattacatg ccagctcctt atccaaatga ggaagcttcg agggccgcca atggtggtgc 540

tcttcctcct gatctttctc tcatcgttaa agcaagacac ggaggagctg attacattat 600

ggctctgctc actggttacc aggatcctcc tgctggtatt caagttcaag agggcatgaa 660

cttcaaccca tatttcccag gtggtggtat tgccatgggt agagttttgt tcgatggtct 720

ggtagaatac gacgatggca ctcctgctac tactacacaa atggctaagg atgtcgctac 780

tttcctcagc tgggctagtg agccagaaca cgacgacaga aagaagatgg gcttccaagc 840

tgtcattatc ctctcagcta tgaccgccat ctcactctac gtcaagagac tcaagtggtc 900

gcctatcaag acgaggaaac tgacttacaa cccaccaaag tgatctgaat gtagagaaaa 960

gtttgacccg tataaaaaat ttcatcctct cctttttccg 1000

<210> 137

<211> 544

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 137

ggaggtagtc cagccaaaaa gagtttgata ggcgcgatgg aggcacaaaa tctcaagact 60

ttcccaaagc aacctatctt ccaaaactca aagacccgtg gtaacaagaa ggtcaccaag 120

gaccgtcgtt ggtacaagga cgtcggtctc ggtttcaaga ctcctcaaga agccatcacc 180

ggtacttaca tcgacaagaa gtgcccatgg accggtgagg tttccatcag aggccgtatc 240

ttgtccggca aggtggtctc taccaagatg acccgtacga tcgtcatcag aagagagtac 300

cttcactacg tgccaaagta caacagatac gagaagcgtc acaagaacct cccagtgcac 360

gcatcacctg cattccgtat cgagaatggt gaccaagtcg tcgttggcca atgccgtcca 420

ctttcaaaga ctgtgagatt caacgtcctc cgtgtcatca agaacaaggc tgctgctaag 480

gctttcgcaa agttctaaac ttgttattaa tgtagttggt ccattcacag aattttgaaa 540

gtcc 544

<210> 138

<211> 938

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 138

acgttcaatt gacttttcca ttcttttgtt cgttctgaag agttttcttt tttctttcat 60

tgtcgcctcc tttctttcgc cttcccgttg tttccgatca tcgggtgttg ccagagtata 120

tatagagttg gggctccctt ttatctatct caccgcaacc gtcctcctga tcctctctcc 180

tgatcctcct ccttcattcc ttgactcctc ttcacgctcc tcctgaccag ccaagtctta 240

catccctctc tacaactact actcttcaaa taatcctctc ctctcggtgg gcttgaatcc 300

cttatcttcc gcctctcccc acgaaccgga ccggatcgtc ttatcgcctc cgcaccagct 360

ggcgcttact acctcatcca cctctttccc gtctcgccac cgaaaccagt ctacaatgcc 420

tcctcgcaag cccagatgct cctttaagga gtgcaaggaa caagcccagc gcattgtcgg 480

agactgcagc ttctgcagcg gtcacttctg ctccaagcat cgcatgctcg aagcccactc 540

ctgctccggt ctggaagact gcaagaagga gtcccacgcc cgcaatgctg ataagttgaa 600

cagcgagcgc acacaggtta tcaagggtgt atgacgggat cacatctaca ctactacaac 660

aatctcggcg catttcgatt gcatttactt gccattttta tccgacgttg agttagcgcg 720

gtgttattta caatttcttc ttttctttat tttgcctacg atgtctcccc ctatcggtat 780

ggtggtgtct cgtttcggga gcgacatggt ttacaatgat tttggtttgg ggtggtctct 840

cggtatttgt ctattatcca cttattttcc ggggtattat gcgcatggcg ttactatatg 900

gagtttgata ttctatctcg aatcgatact tttacaac 938

<210> 139

<211> 687

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 139

aagctctccc aagctaatcg agtttttact gtcacttgca tttatacata ccatgtctag 60

gaacttcggc gatttttcga ctaaccaggc tactcagcag ctgtactccg atagaccctt 120

gcatctccct ggaaatggcc ttaagccggc tagacagctc acgatcagtt cagctgtcgc 180

attccgctgg gactctgacc aaacccgctt caacctgatc agctctgacc gtcgcgaagt 240

gttgcacatc agcattcgcg caaaagacaa cgttctcgtc ctcaacacca aggcgcccga 300

tggtgactgg ggcagggaag agcgacacga gctcaagaaa cttttcgata cccctatgct 360

gccttacatc accgtgacgg cgacgaagat gacctataac atcactgtcc ctagtggtca 420

agaaatcatc ttcaacaaga ggaaaggatt catggaacct gctgtgaaga ttgagtatga 480

ctatgatgag cactctgcgt tctccgaccc atgctacatt acggttccat cttcttaaag 540

gctcgtcggc ttagagttgg cggatagtca cactggtttt tcatacggca aacggcaatg 600

tattacggct tttctctcct gtcctgttca gcggtagatg tacgatgtat gttgtagtgt 660

ttcaaatttg agttcaagtt gttggcc 687

<210> 140

<211> 601

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 140

gtctgcttgc ttcaacgagc gctgaatttc ttgttggccg gtgttgattc cccggattcc 60

ctgttatcgc ctgctgtgtt cgccttggta gcaggtgttt gaattggtcc tgcaatttcg 120

ctgattgctg gcctcgaact cccgatcgaa cgaactctcc tctcctccgt caacgcacct 180

tcacatatcg caaagcacaa tggtttccaa gattctcttc tggagtggct tcggcatcgc 240

cgtccgtctc tggcaactcg gtatcgaaat gcgtcccatt cttgccaagc agggtctctg 300

ggcctacccc gtcttcgcag gtgtcggtgg aagcttcggt tactggctcc agggtgtcga 360

ggaccgtcag ctgaagattc ttgcgcagcg ccgcgaagcc atcctcgaca agcgccggag 420

acgggacgag cgtgagggtc tgagcaacat tgagaaggag ggtactttgg ctgcgacccc 480

atgatttgtt gcgttggctg ttgtttattt tcactgcctt cggagaaaga ccggcaattg 540

cattgctggg catgtatcat accaaaacag aggaaggtta atggtcaatt gtttaatgac 600

c 601

<210> 141

<211> 601

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 141

ggtcattaaa caattgacca ttaaccttcc tctgttttgg tatgatacat gcccagcaat 60

gcaattgccg gtctttctcc gaaggcagtg aaaataaaca acagccaacg caacaaatca 120

tggggtcgca gccaaagtac cctccttctc aatgttgctc agaccctcac gctcgtcccg 180

tctccggcgc ttgtcgagga tggcttcgcg gcgctgcgca agaatcttca gctgacggtc 240

ctcgacaccc tggagccagt aaccgaagct tccaccgaca cctgcgaaga cggggtaggc 300

ccagagaccc tgcttggcaa gaatgggacg catttcgata ccgagttgcc agagacggac 360

ggcgatgccg aagccactcc agaagagaat cttggaaacc attgtgcttt gcgatatgtg 420

aaggtgcgtt gacggaggag aggagagttc gttcgatcgg gagttcgagg ccagcaatca 480

gcgaaattgc aggaccaatt caaacacctg ctaccaaggc gaacacagca ggcgataaca 540

gggaatccgg ggaatcaaca ccggccaaca agaaattcag cgctcgttga agcaagcaga 600

c 601

<210> 142

<211> 380

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 142

gggggcgaca tggcttcgac gccggtgaca atccctgagg tgcatgccga gagcgtaacg 60

tatctcgtaa atccaacgtt acgataaaat agtcgcaaac gacgacaact acgctcaggg 120

cgcgctggca gcgtaacaac tgctagcttc tagtccggcc cggaggtgat gtgcccattc 180

atcaccgaag ggatacgagc tcagactgat gggatcgcgg ctggctttgt cctcgcgtca 240

gccgctaaaa cttagaggaa tcgcgtcgct ggatcctgcc cgtcggagcc agaggcgcta 300

aatcaaaaga cggacctaag catgtagagc cgatgggtga gtgccggcgg acgggggttc 360

aattcccccc gcctccacca 380

<210> 143

<211> 977

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 143

gggaccagaa cagcttcagc tacaatgcca ttcatcaagg aagccaagag caacagctac 60

ttctctcgct accaagtcaa gtaccgcaga cgtcgtgaag gcaagactga cttctacgca 120

cgtaagcgct tggtaacgca agctaagaac aagtacaacg caccaaagta ccgtctcgta 180

gttagattca cgaacaagga catcatctgt caaatcgtgt catcaaagct tcaaggtgac 240

gttgttctca ctcacgctcg cgcccgcgaa cttccacgtt acggcatcaa gcacggtctc 300

acgtcatggt catccgctta cgcggttggt ctcctcgtcg caagaagagc gctcaccaag 360

ctcggtcttg ctgacaagta cgagggtgac gttgaagcta ctggtgaata caacctcacc 420

gagccacttg gcgatgatga accacgtcct ttcaaggtct tccttgacgt tggtcttaag 480

cgtacctcta ctggttctag agtcttcggt gctcttaagg gcgcctcaga cggtggtctc 540

tacatccctc actctgagaa ccgtttccca ggttacgata tcgagagcaa ggaactcgac 600

gctgaaatct tgaacaagta catcttgggt ggtcacattg ctgagtacat ggaggctctt 660

gaggaggaag atgaggagag attcaaggct caattctcta cctatcttga agacggtatt 720

ggatctgagg acattgaaga aatcttctcg ggcgcacacg aggctatccg tgctgaccca 780

accttcaagc caagtgaggc tgccaagggc accgactgga agtccgagtc aaagaagcac 840

cgcgctgtca gactcaccaa gcaacaacgc gaggacgcta tccaacagcg tatcaagtac 900

taccagcaag ctggcgacct cgagtaaacg gtaattgtag cggtctacat agacaatcaa 960

tgtctgttgt tccttag 977

<210> 144

<211> 73

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 144

gattacccgc tgaacttaag catatcaata agcggaggaa aagaaactaa caaggattcc 60

cctagtaacg gcg 73

<210> 145

<211> 823

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 145

gggtctcttc catttgaatt tttcaaccca cagcatggcc ttcatgaatc tcccatggcc 60

cactgaatgc ctacatgccg ctctcaagaa cggatcctta cctttctggg gatttgtaat 120

ctatcgaacg acctacaccg ctcagtcaga tgccgcctgg ccgcagatta tcgagcttat 180

tgcctcctat atgaaagcct tactctacca cgagtataac gacaagaaaa aagatggaga 240

tgagcctaca gtctacgacg aaatctgggc aaggcatcag ttgacgatta tggatgatag 300

acaattcaac ggagcgtctg tgtttgatat ccaacttcac ttcgaaaagt gggttgaggc 360

gcagggaaag cgagatgaat ctactatgta tcgcatgtgt atggtcattg atgatgaatc 420

aatccagacg ttattggagg cgccacccgg ggaaaatagg aaactcggac gacgtatagg 480

gggccctgta cgctttgtca aagtcgtgga ggctttcccc gagctagaca gccttgacga 540

attccaggga tggatgaaat gtgagatcaa cgcgttatgg ccgctgtgga agatgatgtc 600

tgacggagat gaaatgagga tgtcatatga tgagatgaag gggaatggaa agcaggtcta 660

tggcgcaatt taatcggttt ttcttcatgt tatcctgatg gaaaaaatgg cagaacatat 720

gtctgtacat gcagaaaata aggtgattgg aaaatacttg aatgctatga agttagatag 780

tagctgttct agcggccaga taaagccgcg catgtgaatt tcg 823

<210> 146

<211> 181

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 146

gtactaacca ctcgtcaggc gctgaaagaa gaagatgcag taagtttgat gttttctgtg 60

tatgattata cataaagttg ttgtataact acgcagaaaa gttttcgtat gcaaaacttt 120

gattggtgtt aagtcgaaat aaggttcgtg taatggaaat tgcacgggga gtataaaatg 180

t 181

<210> 147

<211> 734

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 147

atgacagaga aactttacac cgagcaagtg aatgcgttcg gaaacgaatt acctcctcta 60

tcatacaaag acctggacaa actcccctta caccaaaacg tcatcaaaga aactcttcga 120

atccacaact caattcatac actcatgcgt aaagtgaaaa atcccctccc agtcccagga 180

acaagattcg ttataccaac cagtcacacc ctcctcgcgt ccccgggcgt aacaacccgc 240

gacgattcac acttccgaaa cgcaatgacc tgggatccac accgttggga aacacgaagt 300

gaggtcgaag atgatggtga gacaatcgat tatggatatg gggttgtttc aaaggggacg 360

aagagtcctt atttgccctt tggagcgggt cgacatcgat gtattgggga gaaattcgca 420

tacttgaatc ttactgttat tgttgctact cttgtgagga attttcggtt ttctgaacct 480

gatgatagag agggtgttcc tgaaacggat tattcgtcac tcttttctag acctatgcgg 540

ccggcgactg ctcggtggga acgacgtggg gagtactaga ggtgggatta ttggggattt 600

gattgctttt tggaattggg atggaagagt tcttgggata tattcttgtt cttcgaggct 660

ttcccaggtg atttttcaca gggcttggta ttatcgtatt taatcaatca attcactaca 720

cttttcgagc ttgc 734

<210> 148

<211> 801

<212> DNA

<213> 从环境挑战生态位纯化的宏基因组

<400> 148

gacatcattg catccaatat cagatcacat tccatagcct catttactgc ccaagaaggc 60

ttatggccca ttggagagag gagtacctaa ctgcactggc agtgcgagat cagagggaga 120

aggccaatct cagtatttac gatgcctata cccgactcgc agatagcacg gccaaacttc 180

cagctacaat agatacaagt ggcagcccct caggtgataa agggccatct ggtacctacg 240

agtccgaaaa gacggcattt tctcaatcaa ggacagccaa gaagcagcag acagaagtgg 300

agccttcagt tacggagctt ctaaatacta cacgtgcaga actagccgaa gcacagcgct 360

ctcgggcaga attgcgagat cgtctagagc gagctactaa cgaagcggag aaattgcgga 420

aacagattgg caaagatggt cgacgaatac atggactgga aaatgaagtt gctcaacagc 480

aaaagcgccg caaagatgtt gaagaagagt tgagaggaaa ggctaagcta ctcaatgaat 540

tccaagacga aattgcagct ctgactctcc aggtgaacat ggccgagaga aaagctaaga 600

agcttggaga ggagaacgat gatcttgtta atcgttggat gaagagaatg ggccaggaag 660

ctgatgcaat gaatgatgcc tccaagtttt cgtgactgcc gaatcagaat agaatcaaat 720

ggcccagata ggccttcgca ttgttatgac atgatcgaat tccgaggcaa attcgcctat 780

catggtaatg aacggataaa g 801

<210> 149

<211> 213

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 149

Met Ala Phe Phe Pro His Tyr Thr Thr Asn Leu Ser Pro Leu Leu Tyr

1 5 10 15

Leu Leu Asp Asp Asp Tyr Ala Val Tyr Arg Ser Thr Cys Pro Lys Ser

20 25 30

Asn Tyr His His Lys Gln His His Ser Arg Arg Gln Pro Ser Pro Val

35 40 45

Arg Tyr Phe Ser Pro Asn Phe Asp Met Arg Glu Gly Asn Asp Ser Tyr

50 55 60

Tyr Leu Asp Gly Glu Leu Pro Gly Val Asn Gln Asn Asp Val Asp Ile

65 70 75 80

Glu Phe Ser Asp Pro Gln Thr Leu Val Ile Lys Gly Arg Val Glu Arg

85 90 95

Asn Tyr Asn Asn Leu Asp Gly Met Asn Glu Glu Asn Gln Gln Asp Glu

100 105 110

Glu Gln Phe Ser Glu Thr Leu Ser Ser Lys Ser Tyr Gln Pro Thr Val

115 120 125

Glu Asp Glu Asp Glu Ala Asn His Ser Pro Pro Val Ala Thr Pro Thr

130 135 140

Tyr Ser Glu Lys Ser Val Thr Glu Lys Thr Gln Lys Pro Ala Tyr Lys

145 150 155 160

Tyr Arg Asn Ser Glu Arg Ala Ile Gly Glu Phe His Arg Ala Phe Asn

165 170 175

Leu Pro Thr Arg Val Asp Gln Asp Ala Val Arg Ala Thr Leu Arg Asn

180 185 190

Gly Ile Leu Ser Leu Glu Leu Pro Lys Glu Pro Ala Pro Lys Met Lys

195 200 205

Lys Ile Arg Ile Glu

210

<210> 150

<211> 164

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 150

Met Gln Leu Leu Ser Thr Leu Thr Pro Leu Ala Leu Leu Val Thr Val

1 5 10 15

Ala Ser Ala Thr Gly Lys Ala Val Asn Asn Ala Val Gly Asn Ala Val

20 25 30

Val Thr Asn His Cys Lys Asp Pro Ile Tyr Leu Trp Ser Val Gly Ser

35 40 45

Ser Val Ser Pro Lys His Thr Ile Pro Ser Gly Ser Asn Tyr Thr Glu

50 55 60

Pro Phe Arg His Asp Asp Ala Ser Gly Gly Ile Ala Leu Lys Ile Thr

65 70 75 80

Arg Asn Asp Asn Gly Leu Tyr Asp Gly Ser Ala Gln Leu Val Tyr Ser

85 90 95

Tyr Ala Leu Asp Gly Glu Gln Val Trp Tyr Asp Leu Ser Ser Val Phe

100 105 110

Gly Asp Ala Phe Ala Gly Glu Ala Val Ala Val Lys Pro Glu Asn Glu

115 120 125

Gly Cys Gly Ser Ile Cys Trp Pro Lys Gly Thr Thr Pro Gly Gly Ser

130 135 140

Gln Val Lys Val Cys Asp Ala Glu Gly Asp Val Gly Leu Val Val Cys

145 150 155 160

Ala Lys Gly Cys

<210> 151

<211> 149

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 151

Leu Leu Ala Glu Ala Ile Arg Arg Gly Leu Leu Gly Trp Arg Arg Ala

1 5 10 15

Glu Ala Lys Trp Gln Arg Cys Cys Cys Trp Leu Ser Thr Gly His Ser

20 25 30

Ala Arg Lys Cys Thr Arg Glu His Ser Leu Val Leu His Glu Arg Leu

35 40 45

Ser Arg Asn Arg Ala Arg His Trp Gln Gly Val Val Ala Leu Cys Leu

50 55 60

Arg Cys Gln Gln Pro Leu Cys Phe Arg Thr Val Ser Phe Ala Leu Thr

65 70 75 80

Val Leu Leu Glu Cys Ile Ile Asn Arg Gln Arg Leu Val His Gln Val

85 90 95

Leu Ala Ile His Ser Arg Asp Cys Leu Val Arg Ala Val Lys Val Val

100 105 110

Val Leu Asp Glu Ser Ile Ser Phe Gln Lys Lys Lys Lys Lys Lys Lys

115 120 125

Lys Lys Thr Gly Gly Gln Val Leu Lys Leu Pro Glu Gly Ile Ser Pro

130 135 140

His Gly Gln Lys Phe

145

<210> 152

<211> 119

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 152

Leu Lys Gly Tyr Gly Phe Ile Gln Tyr Asn Asp Phe Asp Ser Ser Asp

1 5 10 15

Gln Ala Ile Thr Ala Met Asn Gly Gln Tyr Leu Met Asn Lys Pro Leu

20 25 30

Thr Val Asp Tyr Ala Phe Lys Lys Asp Gly Lys Gly Glu Arg His Gly

35 40 45

Thr Glu Ala Glu Arg Leu Leu Ala Ala Glu Ala Lys Arg Asn Asn Ala

50 55 60

Leu Pro Met Pro Gly Ala Ile Pro Gly Gln Pro Phe Met Gln Tyr Gln

65 70 75 80

Gly Met Phe Ala Gly Ala Leu Ser Gly Ala Met Pro Gly Ala Gln Pro

85 90 95

Ala Ala Thr Pro Leu Pro Phe Gly Phe Ser Pro Ala Pro Pro Gln Gln

100 105 110

Ser Thr Pro Tyr Gly Phe Ser

115

<210> 153

<211> 188

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 153

Met Phe Gly Phe Asn Phe Asn Thr Thr Lys Leu Leu Lys Thr Ile Leu

1 5 10 15

Val Val Cys Tyr Leu Gln Ala Thr Val Leu Ala Asp Pro Tyr Thr Arg

20 25 30

Val Ser Trp Glu Ala Tyr Met Asn His Val Asn Gly Ser Asp Asp Tyr

35 40 45

Arg Thr Gln Gly Asp Asp Thr Arg Ala Thr Arg Phe Pro Glu Thr Lys

50 55 60

Pro Pro Lys Gln Gly Lys Asp Phe Leu Trp Ser Ser Lys Pro Val Pro

65 70 75 80

Ser Ser Asp Leu Phe Leu Glu Phe Phe Met Tyr Glu Gly Glu Pro Asp

85 90 95

Glu Phe Ser Arg Thr Thr Glu Ser Tyr Gln Ser Leu Pro Ser Asn Ala

100 105 110

Leu Thr Ala Arg Gln Asn Ala Leu Thr Cys Gln Asp Ile Glu Ser Cys

115 120 125

Ser Tyr Pro Pro Gln Val Asn Asn Phe Gln Ala Leu Phe Asp Asp Leu

130 135 140

Gly Pro Ser Thr Cys Asn Leu Ile Lys Asp Glu Thr Arg Asp Trp Ile

145 150 155 160

Leu Gln Gln Trp Pro Gly Leu Ala Val Gly Ala Val Ile Ser Phe Ala

165 170 175

Val Ala Val Ala Gly Ser Ser Cys Asp Ile Leu Tyr

180 185

<210> 154

<211> 187

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 154

Met Val Arg Tyr Ala His Asn Ala Glu Asn Pro Glu Lys Thr Ala Lys

1 5 10 15

Ala Arg Gly Gln His Leu Arg Thr His Phe Lys Asn Thr Arg Glu Val

20 25 30

Ala Ala Ala Leu Thr Gly Leu Lys Leu Ser Lys Ala Tyr Lys Tyr Leu

35 40 45

Gly Asp Val Gln Glu His Lys Asp Val Ile Pro Phe Arg Arg Phe Asn

50 55 60

Gly Gly Val Gly Arg Ala Ala Gln Ala Lys Asn His Gly Thr Thr Gln

65 70 75 80

Gly Arg Trp Pro Val Lys Ser Ile Gly Phe Leu Leu Arg Leu Leu Lys

85 90 95

Asn Ala Glu Ala Asn Ala Asp Ala Lys Ser Leu Asp Thr Glu Asp Leu

100 105 110

Leu Ile Lys His Ile Val Val Gln Gln Ala Pro Lys Thr Arg Arg Arg

115 120 125

Thr Tyr Arg Ala His Gly Arg Ile Asn Pro Tyr Gln Gly His Pro Cys

130 135 140

His Ile Glu Ile Thr Leu Ala Val Pro Asp Glu Gln Val Ala Arg Asn

145 150 155 160

Lys Asp Val Glu Val Asn Gln Pro Lys Lys Ile Gln Gly Asn Lys Arg

165 170 175

Gln Val Ala Ala Gln Arg Arg Leu Thr Ser Ala

180 185

<210> 155

<211> 103

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 155

Met Thr Gly Arg Gly Lys Gly Gly Lys Gly Leu Gly Lys Gly Gly Ala

1 5 10 15

Lys Arg His Arg Lys Ile Leu Arg Asp Asn Ile Gln Gly Ile Thr Lys

20 25 30

Pro Ala Ile Arg Arg Leu Ala Arg Arg Gly Gly Val Lys Arg Ile Ser

35 40 45

Ala Met Ile Tyr Glu Glu Thr Arg Gly Val Leu Lys Thr Phe Leu Glu

50 55 60

Gly Val Ile Arg Asp Ala Val Thr Tyr Thr Glu His Ala Lys Arg Lys

65 70 75 80

Thr Val Thr Ser Leu Asp Val Val Tyr Ala Leu Lys Arg Gln Gly Arg

85 90 95

Thr Leu Tyr Gly Phe Gly Gly

100

<210> 156

<211> 307

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 156

Met Ser Leu Asp Val Gly Asp Val Asp Ala Trp Ile Asp Thr Leu Ser

1 5 10 15

Gln Cys Lys Gln Leu Ser Glu Ser Asp Val Lys Leu Leu Cys Asp Lys

20 25 30

Ala Arg Glu Ile Leu Ile Glu Glu Ser Asn Val Gln Pro Val Arg Cys

35 40 45

Pro Val Thr Val Cys Gly Asp Ile His Gly Gln Phe His Asp Leu Ile

50 55 60

Glu Leu Phe Arg Ile Gly Gly Asn Ser Pro Ser Thr Asn Tyr Leu Phe

65 70 75 80

Met Gly Asp Tyr Val Asp Arg Gly Tyr Tyr Ser Val Glu Thr Val Thr

85 90 95

Leu Leu Val Ala Leu Lys Leu Arg Tyr Arg Glu Arg Ile Thr Ile Leu

100 105 110

Arg Gly Asn His Glu Ser Arg Gln Ile Thr Gln Val Tyr Gly Phe Tyr

115 120 125

Asp Glu Cys Leu Arg Lys Tyr Gly Asn Ala Asn Val Trp Lys Phe Phe

130 135 140

Thr Asp Leu Phe Asp Tyr Leu Pro Leu Thr Ala Leu Ile Asp Asn Gln

145 150 155 160

Ile Phe Cys Leu His Gly Gly Leu Ser Pro Ser Ile Asp Thr Leu Asp

165 170 175

His Ile Arg Ser Ile Asp Arg Ile Gln Glu Val Pro His Glu Gly Pro

180 185 190

Met Cys Asp Leu Leu Trp Ser Asp Pro Asp Asp Arg Cys Gly Trp Gly

195 200 205

Ile Ser Pro Arg Gly Ala Gly Tyr Thr Phe Gly Gln Asp Ile Ser Glu

210 215 220

Ala Phe Asn His Ser Asn Gly Leu Thr Leu Val Ala Arg Ala His Gln

225 230 235 240

Leu Val Met Glu Gly Tyr Asn Trp Ser Gln Asp Arg Asn Val Val Thr

245 250 255

Leu Phe Ser Ala Pro Asn Tyr Cys Tyr Arg Cys Gly Asn Gln Ala Ala

260 265 270

Ile Met Glu Ile Asp Glu Asn Leu Lys Tyr Thr Phe Leu Gln Phe Asp

275 280 285

Pro Ala Pro Arg Ala Gly Glu Pro Met Val Ser Arg Arg Val Pro Asp

290 295 300

Tyr Phe Leu

305

<210> 157

<211> 103

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 157

Met Thr Gly Arg Gly Lys Gly Gly Lys Gly Leu Gly Lys Gly Gly Ala

1 5 10 15

Lys Arg His Arg Lys Ile Leu Arg Asp Asn Ile Gln Gly Ile Thr Lys

20 25 30

Pro Ala Ile Arg Arg Leu Ala Arg Arg Gly Gly Val Lys Arg Ile Ser

35 40 45

Ala Met Ile Tyr Glu Glu Thr Arg Gly Val Leu Lys Thr Phe Leu Glu

50 55 60

Gly Val Ile Arg Asp Ala Val Thr Tyr Thr Glu His Ala Lys Arg Lys

65 70 75 80

Thr Val Thr Ser Leu Asp Val Val Tyr Ala Leu Lys Arg Gln Gly Arg

85 90 95

Thr Leu Tyr Gly Phe Gly Gly

100

<210> 158

<211> 151

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 158

Gly Ala Thr Thr Thr Ser Ile Leu His His Phe Gln Leu Ser Thr Ser

1 5 10 15

Ser Asn Asn Ser Phe Tyr His Tyr Tyr Leu Asn Asn Leu His Gln Asn

20 25 30

Asp Trp Thr Arg Gln Gly Arg Gln Gly Ser Arg Lys Gly Arg Arg Gln

35 40 45

Ala Ser Pro Gln Asp Leu Ala Arg Gln His Pro Gly His His Gln Ala

50 55 60

Arg His Pro Pro Ser Gly Ala Ser Trp Arg Cys Gln Ala Tyr Leu Arg

65 70 75 80

His Asp Leu Arg Gly Asp Pro Arg Cys Pro Gln Asp Leu Pro Arg Gly

85 90 95

Cys His Pro Arg Arg Arg His Leu His Arg Ala Arg Gln Ala Gln Asp

100 105 110

Arg His Leu Pro Arg Arg Arg Leu Arg Pro Gln Glu Ala Arg Pro His

115 120 125

Pro Leu Arg Phe Arg Trp Leu Ser Ser Ser Leu Phe Ser Leu Arg Leu

130 135 140

Leu Cys Phe Leu Gln Thr Gln

145 150

<210> 159

<211> 146

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 159

Met Phe Ser Lys Leu Ile Ala Ile Ala Ser Leu Ala Leu Ala Ala Asn

1 5 10 15

Ala Ala Val Ile Asp Pro Ser Asp His Thr Val Gln Tyr Glu Ala Ala

20 25 30

Pro Gly Lys Val Val Thr Glu His Tyr Glu Val Leu Ser His Ala Glu

35 40 45

Ala Ser Arg Ile Ile Glu Ala Asn Pro His Ile Ser Asp Tyr Arg Tyr

50 55 60

Arg Cys Asn Tyr Gln Cys Asn Asp Ser Ser Gly Asn Tyr Met Arg Asn

65 70 75 80

Leu Gln Gln Gly Val Pro Asn Gln Ala Cys Ile Phe Ser Ser Cys Tyr

85 90 95

Asp Cys Asp Trp Lys Phe Gln Asn Cys Ser Tyr Cys Arg Leu Ser Thr

100 105 110

Gly His Asn Tyr Arg Asp Ile Gly Gly Leu Glu Ser Trp Cys Tyr Asn

115 120 125

Asn Gly Gly Thr Thr Val Thr His Asn Cys Gly Tyr Thr Asp Gly Asp

130 135 140

Gln Cys

145

<210> 160

<211> 142

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 160

Met His Phe Lys Ser Leu Phe Ile Ala Gly Ala Leu Phe Met Val Gly

1 5 10 15

Ala Ser Ala Val Asp Cys Ala Thr Pro Glu Ile His Cys Glu Thr Ser

20 25 30

Asp Gly Ser Pro Trp Tyr Asp Asp Ala Val Gln Ala Thr Glu Tyr Trp

35 40 45

Lys Glu Ile Gln Asp Ala Gly Lys Asp Ser Cys Gly Asp Ala Gly Cys

50 55 60

Ala Gln Pro His Gly Ser Gly Cys His Ser Asp Gly Gly Ser Tyr Gly

65 70 75 80

Thr Ala Glu Ile Val Leu Cys Gln Asp Asp Ser Ser Ser Ser Thr Pro

85 90 95

Gln Cys Ala Asp Cys Arg Cys Val Tyr Ser Tyr Leu Lys Pro Leu Leu

100 105 110

Asp Gln Cys Lys Gly Ala Asn Asn Lys Ile Gly Gly Tyr Ala His Val

115 120 125

Asp Met Gly Gly Asn Tyr Ile Asn Tyr Glu Phe Val Lys Lys

130 135 140

<210> 161

<211> 100

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 161

Leu Leu Ala Pro Ser Ser Trp Ser Val Pro Val Pro Leu Ile Val Pro

1 5 10 15

Leu Leu Arg Phe Thr Ala Arg Leu Val Met Ala Ala Pro Gly Thr Thr

20 25 30

Met Pro Ser Lys Pro Leu Asn Thr Gly Lys Lys Ser Arg Thr Pro Ala

35 40 45

Lys Thr Ala Ala Val Met Leu Val Ala His Ser Pro Met Ala Leu Asp

50 55 60

Ala Thr Ala Thr Val Val Ala Met Val Pro Pro Arg Ser Phe Ser Ala

65 70 75 80

Arg Met Thr Arg Pro Leu Gln Leu Pro Asn Val Pro Thr Ala Gly Val

85 90 95

Ser Thr Ala Thr

100

<210> 162

<211> 185

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 162

Arg Ala Gly Gly Asn Trp Thr Met Ile Asp Asp Leu Thr Thr Gly Ser

1 5 10 15

Glu Asp Ser Phe Ser Asn Ser Trp Ile Ser Trp Phe Leu Ser Thr Lys

20 25 30

Gly Asn Glu Tyr Phe Cys Glu Val Asp Glu Glu Tyr Ile Leu Asp Arg

35 40 45

Phe Asn Leu Thr Gly Leu Asn Asn Asp Val Gln Asn Tyr Ser Gln Ala

50 55 60

Leu Glu Leu Ile Thr Asp Ser Leu Asp Asp Glu Asp Leu Asp Asp Glu

65 70 75 80

Gln Arg Asp Ala Ile Glu Asn Ser Ala Arg Tyr Leu Tyr Gly Leu Ile

85 90 95

His Ala Arg Tyr Ile Ile Thr Ser Arg Gly Leu Ala Lys Met Leu Phe

100 105 110

Leu Val Tyr Pro Gln Gln Leu Pro Ser Lys Thr Thr Asn Ser Val Pro

115 120 125

Ser Thr Lys Pro Ala Thr Ser Ala Asp Ala Ala Val Gly Val Asp Arg

130 135 140

Tyr Leu Pro Lys Ile Phe Gly Phe Pro Val His Glu Met Ser Lys His

145 150 155 160

Ala Arg Trp Gln Glu Ala Gln Arg Asp Leu Gln Ile Ser Arg Leu Gln

165 170 175

Gln Ser Ala Ser Asp Pro Ser Tyr Val

180 185

<210> 163

<211> 415

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 163

Met Ser Leu Thr Pro Glu Gln Thr Glu Ile Ile Lys Ala Thr Val Pro

1 5 10 15

Val Val Lys Glu His Gly Lys Thr Ile Thr Thr Val Phe Tyr Lys Asn

20 25 30

Met Leu Glu Ala His Pro Glu Leu Asn Ala Ile Phe Asn Thr Thr Asn

35 40 45

Gln Val Asn Gly His Gln Pro Asn Ala Leu Ala Gly Ala Leu Phe Ala

50 55 60

Tyr Ala Ser Asn Ile Asp Asn Leu Gly Ala Leu Gly Pro Ala Val Glu

65 70 75 80

Leu Ile Cys Asn Lys His Ala Ser Leu Tyr Ile Gln Pro Glu His Tyr

85 90 95

Gly Ile Val Gly Lys Phe Leu Leu Glu Ala Met Gly Gln Val Leu Gly

100 105 110

Asp Ala Leu Thr Pro Gln Ile Leu Asp Ala Trp Ala Ala Ala Tyr Trp

115 120 125

Gln Leu Ala Asn Leu Phe Ile Gly Arg Glu Ser Ala Ile Tyr Lys Gln

130 135 140

Ser Glu Gly Trp Thr Gln Trp Arg Glu Phe Arg Val Ala Gln Lys Val

145 150 155 160

Pro Glu Ser Ala Glu Ile Thr Ser Phe Tyr Leu Lys Pro Val Asp Glu

165 170 175

Lys Pro Leu Pro Arg Phe Arg Pro Gly Gln Tyr Ile Ser Val Gln Val

180 185 190

His Val Pro Gln Leu Glu Cys Pro Gln Ala Arg Gln Tyr Ser Leu Ser

195 200 205

Asp Lys Pro Arg Asp Asp Tyr Tyr Arg Ile Ser Val Lys Lys Glu Thr

210 215 220

Gly Leu Asn Thr Ala Lys Pro Glu Ala Lys Val Asn Pro Gly Tyr Val

225 230 235 240

Ser Asn Ile Leu His Glu Asn Val Asn Glu Gly Asp Val Ile Lys Val

245 250 255

Ser His Pro Cys Gly Asp Phe Phe Leu Thr Glu Gln Glu Pro Ser His

260 265 270

Pro Val Val Leu Ile Ala Ala Gly Val Gly Leu Thr Pro Leu Thr Ser

275 280 285

Met Leu Asn Thr Leu Asp Ser Thr Pro Ala Asp Ser Gln Arg Lys Ile

290 295 300

His Phe Ile His Gly Ala Arg Thr Thr Ser Val Arg Ala Phe Lys Asp

305 310 315 320

Gln Ile Lys Ser Arg Ala Glu Arg Leu Pro Asn Leu Gln Ala Thr Phe

325 330 335

Phe Thr Ser Ser Pro Ser Ala Asp Glu Lys Gln Gly Val Asp Tyr Asp

340 345 350

Val Gln Gly Arg Ile Asp Val Ser Lys Met Asp Ala Ser Lys Asp Leu

355 360 365

Phe Leu Asp Asn Ala Gln Thr Glu Phe Tyr Ile Cys Gly Pro Thr Ser

370 375 380

Phe Met Asn Asp Ile Ala Asn Ser Leu Lys Ala Arg Gly Ala Thr Ser

385 390 395 400

Glu Arg Ile His Met Glu Leu Phe Gly Thr Gly Gly Val Pro Val

405 410 415

<210> 164

<211> 212

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 164

Met Ala Phe Met Asn Leu Pro Trp Pro Thr Glu Cys Leu His Ala Ala

1 5 10 15

Leu Lys Asn Gly Ser Leu Pro Phe Trp Gly Phe Val Ile Tyr Arg Thr

20 25 30

Thr Tyr Thr Ala Gln Ser Asp Ala Ala Trp Pro Gln Ile Ile Glu Leu

35 40 45

Ile Ala Ser Tyr Met Lys Ala Leu Leu Tyr His Glu Tyr Asn Asp Lys

50 55 60

Lys Lys Asp Gly Asp Glu Pro Thr Val Tyr Asp Glu Ile Trp Ala Arg

65 70 75 80

His Gln Leu Thr Ile Met Asp Asp Arg Gln Phe Asn Gly Ala Ser Val

85 90 95

Phe Asp Ile Gln Leu His Phe Glu Lys Trp Val Glu Ala Gln Gly Lys

100 105 110

Arg Asp Glu Ser Thr Met Tyr Arg Met Cys Met Val Ile Asp Asp Glu

115 120 125

Ser Ile Gln Thr Leu Leu Glu Ala Pro Pro Gly Glu Asn Arg Lys Leu

130 135 140

Gly Arg Arg Ile Gly Gly Pro Val Arg Phe Val Lys Val Val Glu Ala

145 150 155 160

Phe Pro Glu Leu Asp Ser Leu Asp Glu Phe Gln Gly Trp Met Lys Cys

165 170 175

Glu Ile Asn Ala Leu Trp Pro Leu Trp Lys Met Met Ser Asp Gly Asp

180 185 190

Glu Met Arg Met Ser Tyr Asp Glu Met Lys Gly Asn Gly Lys Gln Val

195 200 205

Tyr Gly Ala Ile

210

<210> 165

<211> 222

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 165

Met Ala Arg Arg Pro Ala Arg Cys Tyr Arg Tyr Cys Lys Asn Lys Pro

1 5 10 15

Tyr Pro Lys Ser Arg Phe Asn Arg Gly Val Pro Asp Pro Lys Ile Arg

20 25 30

Ile Phe Asp Leu Gly Arg Lys Lys Ala Ser Val Asp Asp Phe Pro Leu

35 40 45

Cys Val His Met Val Ser Asn Glu Tyr Glu Gln Leu Ser Ser Glu Ala

50 55 60

Leu Glu Ala Ala Arg Ile Cys Ala Asn Lys Tyr Leu Val Lys Ile Ala

65 70 75 80

Gly Lys Glu Gly Phe His Leu Arg Val Arg Ala His Pro Phe His Val

85 90 95

Val Arg Ile Asn Lys Met Leu Ser Cys Ala Gly Ala Asp Arg Leu Gln

100 105 110

Thr Gly Met Arg Gly Ala Phe Gly Lys Pro Asn Gly Val Val Ala Arg

115 120 125

Val Asn Ile Gly Gln Ile Leu Leu Ser Ile Arg Thr Arg Asp Ser Asn

130 135 140

Arg Ala Ala Ala Val Glu Ala Met Arg Arg Ser Thr Tyr Lys Phe Pro

145 150 155 160

Gly Arg Gln Lys Ile Ile Ile Ser Lys Asn Trp Gly Phe Thr Pro Val

165 170 175

Arg Arg Glu Glu Tyr Val Lys Leu Arg Gln Glu Gly Lys Leu Lys Gln

180 185 190

Asp Gly Ala Tyr Val Gln Phe Leu Arg Gly His Gly Leu Val Glu Glu

195 200 205

Asn Met Lys Arg Phe Pro Gln Ala Tyr Glu Gly Val Ala Gln

210 215 220

<210> 166

<211> 252

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 166

Met Ser Phe Tyr Gln Ser Arg Pro Asp Thr Ile Lys Gly Pro Asp Pro

1 5 10 15

Leu Thr Asp Asn Trp Thr Tyr Asp Ser Ala Ile Asp Leu Phe Ser Trp

20 25 30

Asn Pro Met Met Pro Asp Pro Phe Thr Phe Asp Leu Pro Asp Asp Leu

35 40 45

Met Lys Phe Glu Ser Lys Asp Met Ser Ala Gly Met Val Ala Pro Ser

50 55 60

Asp Ile Ser Gly Phe Ala Ile Gly Asn His Leu Gly Glu Asp Ala Ala

65 70 75 80

Ser Ile Ser Asp Pro Glu Ser Asp Asp His Pro Trp Ser Pro Ser Ala

85 90 95

His Ala Ala Phe Pro Glu Leu Ser Pro Ile Thr Ser Thr Glu Gln Val

100 105 110

His Gln Glu Thr Ala Arg Tyr Ser Thr Thr Pro Asp Ala Thr Ser Pro

115 120 125

Gln Glu Gln Pro Ser Ser Pro Pro Thr Arg Ser Thr Arg Arg Arg Ser

130 135 140

Ser Ala Asp Gly Pro Val Arg Asn Ala Ala Lys Arg Ala Ala His Asn

145 150 155 160

Val Ile Glu Lys Arg Tyr Arg Thr Asn Met Asn Ala Lys Phe Val Ala

165 170 175

Leu Glu Lys Ala Met Asn Gly Gly Asn Gly Val Gln Thr Ser Ser Arg

180 185 190

Gly Gly Gly Ser Ala Ser Leu Lys Lys Ser Glu Ile Leu Ser Asn Ala

195 200 205

Ile Ala Tyr Met His Gly Leu Gln Glu Glu Asn Arg Tyr Leu Gln Lys

210 215 220

Glu Leu Ala Ile Val Lys Gln Asn Leu Val Pro Ala Gly Ile Trp Arg

225 230 235 240

Gly Ala Pro Ser Cys Lys Arg Glu Thr Ser Tyr Arg

245 250

<210> 167

<211> 254

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 167

Met Gly Arg Val Ile Arg Asn Gln Arg Lys Gly Arg Gly Ser Ile Phe

1 5 10 15

Thr Ala His Thr Arg Leu Asn Lys Ala Pro Ala Gln Phe Arg Thr Leu

20 25 30

Asp Phe Ala Glu Arg His Gly Tyr Thr Arg Gly Val Val Lys Glu Ile

35 40 45

Ile His Asp Ala Gly Arg Gly Ala Pro Leu Ala Lys Val Gln Phe Arg

50 55 60

His Pro Tyr Lys Phe Lys Met Val Thr Glu Thr Phe Ile Ala Asn Glu

65 70 75 80

Gly Met Tyr Thr Gly Gln Phe Ile Tyr Ala Gly Lys Asn Ala Gln Leu

85 90 95

Thr Val Gly Asn Val Leu Pro Leu Ala Ser Met Pro Glu Gly Thr Val

100 105 110

Ile Ser Asn Val Glu Glu Lys Ser Gly Asp Arg Gly Ala Leu Gly Arg

115 120 125

Thr Ser Gly Asn Tyr Val Thr Val Ile Gly His Asn Pro Glu Asp Gly

130 135 140

Lys Thr Arg Val Lys Leu Pro Ser Gly Ala Lys Lys Val Ile Lys Asn

145 150 155 160

Thr Ala Arg Gly Met Val Gly Ile Val Ala Gly Gly Gly Arg Thr Asp

165 170 175

Lys Pro Leu Leu Lys Ala Ser Arg Ala Lys His Lys Phe Ala Val Lys

180 185 190

Arg Asn Ser Trp Pro Lys Thr Arg Gly Val Ala Met Asn Pro Val Asp

195 200 205

His Pro His Gly Gly Gly Asn His Gln His Ile Gly Lys Ala Ser Thr

210 215 220

Ile Ser Arg Tyr Ala Ala Gln Gly Gln Lys Ala Gly Leu Ile Ala Ala

225 230 235 240

Arg Arg Thr Gly Leu Leu Arg Gly Thr Gln Lys Thr Lys Asp

245 250

<210> 168

<211> 249

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 168

Gly Ala Glu Ala Tyr Tyr Ser Pro Val Ser Ser Leu Ile Gly Met Ser

1 5 10 15

Thr Gly Leu Arg Phe Ser Thr Leu Pro Ala Ala Ser Asn Pro Gln Ser

20 25 30

Ser Ser Leu Ile Pro Ser Pro Ser Ala Pro Ile Ser Thr Phe Pro Tyr

35 40 45

Thr Leu Thr Leu Thr Leu Thr Pro Leu Thr Gly Ser Leu Ser Thr Ser

50 55 60

Tyr Ser Leu Arg Ala Ser Pro Asn Leu Ser Phe Ser Ser Arg Phe Gly

65 70 75 80

Phe Asn Val Tyr Ser Trp Glu Ser Glu Met Val Ala Gly Phe Glu Leu

85 90 95

Trp Arg Gln Ser Lys Lys Pro Lys Leu Ala Ala Gly Ser Asp Gly Asp

100 105 110

Asp Leu Glu Trp Ala Arg Arg Lys Val Arg Val Trp Asp Pro Ser Ala

115 120 125

Phe Pro Leu Ala Pro Pro Glu Pro Glu Ile Pro Gln Pro Asn His Glu

130 135 140

Asp Glu Ser Gln Glu Ser Val Leu Lys Leu Arg Val Asp Gln Ser Trp

145 150 155 160

Asn Val Arg Leu Leu Trp Glu Gly Arg Val Lys Glu Leu Leu Val Ser

165 170 175

Ala Gly Val Gly Leu Gly Pro Ser Ser Phe Ser Pro Ser Ser Tyr Ala

180 185 190

Asn Pro Pro Gly Thr Ala Gly Ala Gln Gly Ser Gly Gly Gly Ser Pro

195 200 205

Ala Ser Tyr Trp Arg Gly Val Gly Gly Phe Gly Ile Ile Phe Phe Met

210 215 220

Arg Asp Phe Phe Gly Ser Met Tyr Leu Asn Glu His Cys Leu Asp Val

225 230 235 240

Tyr Ser Leu Ser Asp Phe Met Arg Gln

245

<210> 169

<211> 211

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 169

Gly Pro Gly Val Leu Ser Gly Phe Gln Pro Pro Asp Leu Pro Leu Ile

1 5 10 15

Thr Ser Ile Glu Leu Asp Leu Asp Val Val Leu Pro Pro Pro Ala Cys

20 25 30

Arg Thr Met Phe Leu Arg Thr Val Ser Arg Ala Val Pro Arg Ser Thr

35 40 45

Ala Ala Ile Arg Ala Ala Pro Thr Ala Ser Val Asn Ala Leu Gln Thr

50 55 60

Arg Ala Ala Ser Asp His Ala Ile Pro Asn Pro Thr Leu Ala Asn Ile

65 70 75 80

Glu Lys Arg Trp Glu Val Met Pro Pro Gln Glu Gln Ala Glu Leu Trp

85 90 95

Met Gln Leu Arg Asp Arg Met Lys Val Asp Trp His Gln Met Thr Leu

100 105 110

Gln Glu Lys Lys Ala Ala Tyr Tyr Ile Ala Phe Gly Ala His Gly Pro

115 120 125

Arg Ala Gln Pro Pro Lys Gly Glu Gly Met Arg Val Phe Ala Lys Val

130 135 140

Leu Gln Leu Thr Ala Ala Ser Val Ala Val Phe Tyr Ala Ile His Ala

145 150 155 160

Phe Ala Gly Lys Gln Pro Ala Thr Met Ser Lys Glu Trp Gln Glu Ala

165 170 175

Ser Asn Glu Tyr Ala Leu Lys Glu Lys Ile Asn Pro Ile His Gly Ile

180 185 190

Ser Lys Glu Gly Tyr Glu Gly Lys Gly Phe Val Gln Ser Pro Pro Ala

195 200 205

Glu Lys Ser

210

<210> 170

<211> 153

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 170

Val Ala Arg Arg Gly Gly Ile Tyr Leu Asp Gly Asn Asn Asp Leu Val

1 5 10 15

Thr Met Lys Gly Asn Tyr Ile Tyr His Thr Ser Gly Arg Ser Pro Lys

20 25 30

Val Gln Gly Asn Thr Leu Leu His Ala Val Asn Asn Tyr Trp His Asp

35 40 45

Asn Ser Gly His Ala Phe Glu Ile Gly Glu Gly Gly Tyr Val Leu Ala

50 55 60

Glu Gly Asn Val Phe Gln Asp Val Thr Thr Pro Val Glu Asp Pro Val

65 70 75 80

Asp Gly Gln Leu Phe Thr Ser Pro Asp Pro Ser Thr Asn Ala Gln Cys

85 90 95

Ser Ser Tyr Leu Gly Arg Ala Cys Glu Ile Asn Gly Phe Gly Asn Ser

100 105 110

Gly Thr Phe Asn Gln Ala Asp Thr Ser Leu Leu Ser Lys Phe Lys Gly

115 120 125

Gln Asn Ile Ala Ser Ala Asp Ala Tyr Ser Lys Val Ala Ser Ser Val

130 135 140

Ala Ser Asn Ala Gly Gln Gly His Leu

145 150

<210> 171

<211> 120

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 171

Val Arg Val Val Thr Phe Trp Pro Arg Val Thr Ser Ser Arg Met Leu

1 5 10 15

Leu Pro Pro Leu Arg Thr Pro Leu Thr Ala Ser Ser Ser Leu Pro Leu

20 25 30

Thr Pro Ala Pro Thr Leu Ser Ala Arg His Thr Leu Ala Gly Pro Ala

35 40 45

Lys Ser Thr Ala Ser Val Thr Leu Val Pro Ser Thr Arg Leu Thr Leu

50 55 60

Ala Cys Cys Leu Asn Leu Arg Val Arg Thr Leu Leu Leu Leu Met Leu

65 70 75 80

Thr Leu Arg Leu Pro Arg Ala Leu Pro Ala Thr Pro Val Arg Asp Thr

85 90 95

Cys Lys Met Glu Arg Gly Gly Ser Glu Leu Asn Leu Leu Met Ser Asp

100 105 110

Asp Ile Ala Leu Ala Ala Cys Trp

115 120

<210> 172

<211> 80

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 172

Met Pro Ser Lys Thr Glu Ala Ala Arg Leu Gln Asn Asp Phe Gly Ala

1 5 10 15

Asp Tyr Trp Val Arg Asn Thr Gln Glu Arg Arg His Ser Thr Ala Gly

20 25 30

Arg Gly Leu Phe Ala Gly Leu Gln Asp Val Lys His Tyr Asn Val Asp

35 40 45

His Gly Trp Ala Arg Arg Lys Ser Ser Asp Asn Pro Gly Leu Leu Ala

50 55 60

Ser Phe Phe Ser Arg Phe Thr Gly Gly Ser Tyr His Pro Pro Ser Glu

65 70 75 80

<210> 173

<211> 137

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 173

Val Ala Arg Arg Asp Ser His His Gln Ile Lys Ser Arg Leu Ile Leu

1 5 10 15

Leu Asp Ile Thr Thr Ile Gln His Lys Val Pro Ser Tyr Phe Lys Gln

20 25 30

Ile Ser Thr Ile Glu Ser Lys Cys His Pro Lys Pro Lys Gln Pro Val

35 40 45

Tyr Lys Thr Thr Ser Ala Gln Thr Thr Gly Leu Glu Ile Pro Lys Asn

50 55 60

Ala Ala Thr Gln Pro Leu Ala Ala Asp Tyr Ser Pro Val Ser Arg Met

65 70 75 80

Ser Ser Thr Ile Thr Ser Thr Met Ala Gly Pro Val Ala Ser Leu Ala

85 90 95

Ile Thr Pro Asp Ser Leu Leu Leu Ser Ser Val Asp Ser Pro Gly Asp

100 105 110

His Thr Ile Arg Pro Arg Asn Arg Ile Pro Phe Leu Asn Val Arg Tyr

115 120 125

Trp Glu Glu Cys Asp Leu Asn Trp Glu

130 135

<210> 174

<211> 181

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 174

Val Val Ser Thr Gln Ser Asp Glu Pro Thr Ile Pro Gly Gly Ala Ala

1 5 10 15

Val Thr Ile His Ser Arg Asn Glu Lys Lys Ala Arg Lys Ala Ile Gly

20 25 30

Lys Leu Gly Leu Lys His Val Pro Gly Ile Thr Arg Val Thr Leu Arg

35 40 45

Arg Pro Lys Asn Ile Leu Phe Val Val Asn Gln Pro Asp Val Tyr Lys

50 55 60

Ser Pro Ser Ser Asn Thr Trp Ile Ile Phe Gly Glu Ala Lys Ile Glu

65 70 75 80

Asp Leu Asn Ser Gln Ala Gln Ala Ser Ala Ala Gln Gln Leu Ala Ala

85 90 95

Ala Glu Ala Ala Ala Gly Gly Glu His Ala Gly His Asp His Glu His

100 105 110

Asp Ile Leu Gly Lys Gly Lys Ala Pro Glu Thr Glu Gly Lys Lys Glu

115 120 125

Glu Glu Glu Asp Asp Gly Glu Glu Val Asp Glu Ala Gly Leu Glu Ala

130 135 140

Lys Asp Ile Asp Leu Val Met Ala Gln Ala Asn Val Ser Arg Lys Lys

145 150 155 160

Ala Val Lys Ala Leu Arg Glu Asn Asp Asn Asp Ile Val Asn Ser Ile

165 170 175

Met Ala Leu Ser Ile

180

<210> 175

<211> 191

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 175

Met Phe Leu Gln Arg Thr Val Ser Thr Leu Ala Arg Arg Thr Pro Val

1 5 10 15

Arg Gly Leu Ala Ala Ala Arg Pro Phe Ser Ser Ser Val Ser Arg Phe

20 25 30

Asn Lys Tyr Glu Val Lys Glu Ala Lys Leu Arg Ser Leu Asp Glu Ile

35 40 45

Gln Thr Glu Glu Asp Leu Ile Pro Pro Gly Ala Lys Pro Gly Thr Val

50 55 60

Pro Ser Asp Ile Glu His Ala Thr Gly Leu Glu Arg Leu Glu Leu Val

65 70 75 80

Gly Lys Met Gln Gly Ile Asp Ile Phe Asp Leu Arg Pro Leu Asp Ala

85 90 95

Ser Arg Lys Gly Thr Leu Glu Asn Pro Ile Val Val Asn Gly Ala Gly

100 105 110

Asp Glu Gln Tyr Ala Gly Cys Thr Gly Tyr Pro Val Asp Ser His Gln

115 120 125

Val Asn Trp Leu Thr Val Ser Arg Glu Arg Pro Ile Glu Arg Cys Asn

130 135 140

Glu Cys Gly Asn Val Val Lys Leu Asn Tyr Val Gly Pro Glu Glu Asp

145 150 155 160

Pro His Ala His Asp His Gly His Gly His His Pro Ala Pro Glu Glu

165 170 175

Pro Lys Thr Phe Ala Asp Tyr Val Lys Pro Glu Tyr Trp Tyr Arg

180 185 190

<210> 176

<211> 261

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 176

Met Asn Pro Tyr Ile Val Asp Pro Met Leu Lys Tyr Val Ala Phe Asp

1 5 10 15

Ile Pro Ala Leu Ala Arg Leu Asp Pro Ser Leu Cys Leu Phe Leu Ile

20 25 30

Leu Phe Phe Glu Asn Ser Arg Val Val Leu Leu Gly Tyr Leu Pro Val

35 40 45

Pro Val Leu Gly Leu Asp Val Val Gly Glu Gly Leu Gly Leu Leu Gly

50 55 60

Gly Arg Val Val Ala Val Ala Val Val Val Ser Val Arg Val Leu Leu

65 70 75 80

Arg Ser Asp Ile Val Gln Leu Asp Asn Val Thr Ala Phe Val Ala Ala

85 90 95

Leu Asp Gly Ala Leu Thr Arg Asp Ser Gln Pro Val Asn Leu Val Arg

100 105 110

Val Asp Gly Val Thr Ser Ala Thr Ser Val Leu Leu Val Thr Ser Thr

115 120 125

Val Asp Asn Asn Gly Val Phe Glu Gly Ser Leu Ala Gly Ser Ile Gln

130 135 140

Arg Pro Gln Val Glu Asp Val Asn Ser Leu His Phe Thr Asp Glu Phe

145 150 155 160

Glu Thr Leu Glu Thr Ser Gly Val Phe Asp Ile Ala Arg Asp Gly Thr

165 170 175

Gly Leu Ser Thr Arg Gly Asp Glu Val Phe Phe Ser Leu Asp Leu Val

180 185 190

Lys Arg Thr Glu Leu Gly Leu Leu Asn Leu Val Leu Val Glu Ser Ala

195 200 205

Asn Gly Arg Arg Lys Arg Ala Arg Gly Ser Lys Ala Pro His Gly Gly

210 215 220

Ala Pro Arg Glu Gly Arg Tyr Arg Thr Leu Lys Glu His Ser Pro Cys

225 230 235 240

Cys Asn Arg Thr Gly Arg Ser Asn Gly Arg Gly Glu Gly Gly Gly Gly

245 250 255

Val Asp Val Gly Ser

260

<210> 177

<211> 221

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 177

Met Ala Asp Ile Thr Ala Val Gly Glu Glu Asn Pro Ser Pro Thr Gln

1 5 10 15

Asp Glu Leu Gln Gln Ala Ala Ala Gly Asn Gly Ala Pro Asp Asn Arg

20 25 30

Thr Pro Lys Arg Arg Met Ser Asp Asp Glu Glu Asp Glu Glu Lys Gln

35 40 45

Gly Arg Glu Arg Arg Lys Ile Glu Ile Lys Phe Ile Gln Asp Lys Ser

50 55 60

Arg Arg His Ile Thr Phe Ser Lys Arg Lys Ala Gly Ile Met Lys Lys

65 70 75 80

Ala Tyr Glu Leu Ser Val Leu Thr Gly Thr Gln Val Leu Leu Leu Val

85 90 95

Val Ser Glu Thr Gly Leu Val Tyr Thr Phe Thr Thr Pro Lys Leu Gln

100 105 110

Pro Leu Val Thr Lys Ala Glu Gly Lys Asn Leu Ile Gln Ala Cys Leu

115 120 125

Asn Ala Pro Asp Pro Thr Thr Ser Glu Asn Gly Val Asp Ala Pro Glu

130 135 140

Val Pro Ala Glu Thr Pro Glu Asp Val Asn His Ala Asn Val Asn Ala

145 150 155 160

Ala Ala Ala Gln Gln Thr Asn Ile Pro Arg Pro Thr Gly Met His Pro

165 170 175

Gly Tyr Met Thr Asn Glu Gln Gln Gln Gln Met Ala Tyr Tyr Gln Asn

180 185 190

His Leu Gln Gln Gln Gln Gln Ala Gly Gly Gln Tyr Pro Gly Met Ser

195 200 205

Val Gly Gly Arg Met Pro Thr Gln His Gln Pro Thr Ala

210 215 220

<210> 178

<211> 176

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 178

Met Ser Phe Ala Ala Asp Pro Pro Pro Asn Asn His Gly Thr Leu Thr

1 5 10 15

His Leu Phe Arg Ala Pro Glu Asp Leu Val Tyr Pro Ile Pro Glu Asn

20 25 30

Phe Ser Leu Glu Glu Thr Val Leu Val Glu Pro Leu Ser Val Ala Ile

35 40 45

His Gly Ala Arg Val Ala Gly Ile Thr Pro Gly His Thr Val Leu Val

50 55 60

Gln Ala Ser Gly Thr Ile Gly Leu Phe Cys Ala Ala Thr Ala Thr Ala

65 70 75 80

Phe Gly Ala Lys Gln Val Ile Ile Ser Asp Ile Asn Gln Thr Lys Leu

85 90 95

Asp Phe Ala Arg Asp Tyr Leu Gly Cys Pro Ile Phe Leu Pro Asn Ile

100 105 110

Ser Ser Ser His Pro Glu Glu Glu Ala Ser Arg Met Lys Glu Tyr Ile

115 120 125

Glu Ser Gln Arg Arg Cys Arg Tyr Ser Ser Val Val Tyr Gly Ser Gly

130 135 140

Ile Leu Val Ala Lys Ser Gly Pro Gly Gly Val Val Val Pro Asn Trp

145 150 155 160

Thr Arg Phe Asn Arg Val Gln Ser Asn Gly Thr Tyr His Gln Tyr Val

165 170 175

<210> 179

<211> 86

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 179

Met Pro Arg Gly Ala Glu Tyr Ala Asn Gly Pro Leu Gln Ser Asp Asn

1 5 10 15

Ala Ile Glu Ala Gly Glu Asn Lys Ala His Gly Thr Ser Gly Asn Thr

20 25 30

Gly Leu Asn Arg Val Asn Lys Val Ala Glu Phe Pro Glu Gly Ala Arg

35 40 45

Gly Thr Gly Thr Ala Ala Asn Pro Leu Ser Gly Gln Gly Ser Ala Gly

50 55 60

His Gln Asp Gly Lys Gly Gly His Asp Pro Lys Thr Leu Gly Glu Asn

65 70 75 80

Lys Gly Leu Gly Thr Gln

85

<210> 180

<211> 78

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 180

Met Pro Ser Lys Leu Ala Lys Ile Arg Pro Thr Glu Pro Pro Val Thr

1 5 10 15

Pro Ala Ser Thr Ala Ser Thr Arg Ser Pro Asn Ser Pro Lys Ala Pro

20 25 30

Glu Glu Pro Val Pro Leu Leu Thr Arg Ser Val Ala Arg Val Ala Pro

35 40 45

Ala Ile Arg Met Glu Arg Val Ala Met Thr Arg Arg Pro Leu Glu Arg

50 55 60

Thr Arg Asp Trp Val Leu Asn Asp Leu Met Ile Gln Lys Thr

65 70 75

<210> 181

<211> 166

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 181

Met Thr Thr Ile Thr Glu Phe Pro Pro Phe Tyr Thr Gln Gln Pro Asn

1 5 10 15

Ala Ser Ala Leu Thr Gln Gln Leu Gly Leu Trp Gln Lys His Ile Leu

20 25 30

Ser Thr Cys Lys Gln Arg Arg Gln Phe Lys Leu Ser Val Ser Asp Asp

35 40 45

Ile Trp Ala Asn Glu Arg Ile Lys Arg Ala Ala Ser Arg Glu Phe Ile

50 55 60

Ser Val Ile Ile Ser Ser Leu Val Thr Glu Gly Leu Ala Ser Tyr Thr

65 70 75 80

Asp Ala Thr Lys Glu Ala Val Trp Val Tyr Trp Arg Ser Leu Ser Asp

85 90 95

Trp Ala Gln Ala Ala Tyr Ala Tyr Ala Glu Ser Thr Ala Gln Leu Asn

100 105 110

Thr Pro Leu Thr Tyr Tyr Glu Leu Val Gln Gly Glu Tyr Ser His Leu

115 120 125

Ser Glu Leu His Glu Met Pro Val Glu Leu Leu Lys Leu Ala Val Ser

130 135 140

Leu Leu Val Lys Gln Asn Lys Ala Val Ile Ile Lys Thr Ser Gln Gly

145 150 155 160

Glu Gly Val Lys Phe Val

165

<210> 182

<211> 130

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 182

Met Ser Ile Pro Lys Ala Ala Ala His Thr Asp Lys Ala Pro Gln Pro

1 5 10 15

Phe Lys Asp Leu Tyr Ser Gln Ala Val Ile Ala Gly Gly Val Val Tyr

20 25 30

Cys Ser Gly Ile Val Ala Ile Asp Pro Glu Thr Gly Ser Leu Ile Glu

35 40 45

Gly Asp Val Lys Ala His Thr Glu Arg Ile Leu Gln Ser Leu Ser Ser

50 55 60

Thr Leu Gln Ala Ala Gly Thr Ser Leu Asp Arg Ala Val Lys Ile Asn

65 70 75 80

Val Tyr Leu Ala Asn Met Glu Asp Phe Thr Ser Met Asn Ser Val Tyr

85 90 95

Glu Lys Tyr Phe Val Asp Gly Val Lys Pro Cys Arg Thr Cys Val Ala

100 105 110

Val Lys Ser Leu Pro Phe Gly Thr Asp Val Glu Met Glu Cys Ile Ala

115 120 125

Val Leu

130

<210> 183

<211> 113

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 183

Met Leu Arg Ser Gln Phe Gly Val Ile Ser Asn Ala Ala Lys Thr Ala

1 5 10 15

Ala Phe Leu Lys Pro Val Gln Thr Arg Leu Tyr Ala Ser Gly Ala Leu

20 25 30

Ser Lys Gly Asp Ile Gln Thr Arg Ile Phe Asp Val Leu Lys Ser Phe

35 40 45

Asp Lys Val Lys Ala Asp Asn Leu Thr Glu Ser Ala Ser Phe Thr Asn

50 55 60

Asp Leu Gly Leu Asp Ser Leu Asp Ala Val Glu Val Val Met Ala Ile

65 70 75 80

Glu Glu Glu Phe Ala Ile Glu Ile Pro Asp Ala Glu Ala Asp Ala Ile

85 90 95

Gln Asn Val Asn Gln Ala Ile Glu Tyr Ile Ala Lys Thr Pro Glu Ala

100 105 110

His

<210> 184

<211> 200

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 184

Val Val Ser Thr Gln Ser Gly Ala Pro Gly Thr Lys Lys Val Ser Ser

1 5 10 15

Thr Tyr Leu Ser Lys Ile Cys Lys Glu Gln His Lys Ser Ile Phe Phe

20 25 30

Tyr Leu Ile Asp Leu His Ser Ser Ala Ser Leu Arg Ser Val Thr Thr

35 40 45

Leu Pro Ala Arg Met Glu Arg Ile Arg Leu Ser Glu Gln Ala Ala Thr

50 55 60

Ile Cys Asn Gln Ile Arg Glu Met Ile Pro Glu Thr Ala Thr Leu Pro

65 70 75 80

Asn Gln Pro Gly Lys Asp Gln Ala Glu Leu Met His Glu Asp Glu Asn

85 90 95

Gly Asn Lys Ile Tyr Gly Gly Lys Leu Leu Thr Glu Arg Ala Ala Arg

100 105 110

Leu Lys Glu His Met Lys Ile Asp Gln Val Ser Ala Arg Phe Ile Ser

115 120 125

Gln Tyr Phe Thr Asn Gly Ile Gln Asp Trp Thr Glu Arg Leu Val Tyr

130 135 140

Trp Thr Lys Pro Thr Lys Leu Leu Asn Gln Arg Lys Gln Gly Tyr Ile

145 150 155 160

Ile Pro Leu Ser Lys Asp Ile Val Leu Gln Pro Gly Gly Pro Leu Glu

165 170 175

Ala Asn Asn Gly Phe Arg Val Thr Asn Glu Arg Ile Leu Ser Ser Gly

180 185 190

Ala Ala Leu Phe Ile Met Pro Gln

195 200

<210> 185

<211> 524

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 185

Met Leu Ala Arg Ser Leu Gln Gln Ile Arg Arg Ser Ser Arg Leu Ser

1 5 10 15

Leu Gln Leu Arg Ala Tyr Ala Ser Ser Pro Asp Arg Ser Ala Ser Phe

20 25 30

Ser Lys Leu Ser Glu Gln Asp Leu Pro Ser Leu Ala Ser Ile Phe Ser

35 40 45

Ser Pro Asp Thr Ser Leu Leu Thr Thr Leu Gly Asp Lys Pro Thr Ala

50 55 60

Thr Ser Asp Asp Leu Glu Pro Phe Asn Val Asp Trp Met Gly Lys Tyr

65 70 75 80

Lys Gly His Ser Ser Ile Ile Val Lys Pro Lys Thr Thr Gln Glu Val

85 90 95

Ser Lys Val Leu Gln Trp Cys Asn Glu Arg Asn Val Ala Val Val Pro

100 105 110

Gln Gly Gly Asn Thr Gly Leu Val Gly Gly Ser Val Pro Leu His Asp

115 120 125

Glu Val Val Leu Ser Leu Ser Ser Met Asn Ser Ile Arg His Phe Asp

130 135 140

Pro Leu Ser Gly Tyr Val Ser Val Asp Ser Gly Ile Val Leu Glu Asn

145 150 155 160

Leu Asp Asn Tyr Leu Ala Gln Gln Gly His Ile Val Pro Leu Asp Leu

165 170 175

Gly Ala Lys Gly Ser Cys Gln Ile Gly Gly Asn Val Ala Thr Asn Ala

180 185 190

Gly Gly Leu Arg Met Leu Arg Tyr Gly Ser Leu His Gly Asn Val Leu

195 200 205

Gly Leu Glu Val Val Leu Pro Asp Gly Arg Val Ile Asn Gly Met Lys

210 215 220

Gly Leu Lys Lys Asp Asn Thr Gly Ile Asp Leu Lys Gln Leu Phe Ile

225 230 235 240

Gly Ser Glu Gly Val Leu Gly Val Ile Thr Gly Val Thr Leu Ala Thr

245 250 255

Pro Val Arg Pro Ser Ala Thr Asn Val Ala Val Phe Ala Leu Pro Asp

260 265 270

Tyr Glu Ser Val Gln Thr Ala Phe Ser Ser Ala Arg Arg Asp Leu Gly

275 280 285

Glu Ile Leu Ser Ala Phe Glu Phe Phe Asp Ala Ala Ser Tyr Lys Leu

290 295 300

Val Arg Ser His Gly His Ala Ala Glu Arg Lys Thr Phe Glu Asp Gly

305 310 315 320

Glu Asp Ala Pro Phe Phe Cys Leu Val Glu Thr Ser Gly Ser Asn Lys

325 330 335

Asp His Asp Asp Glu Lys Leu Gly Ala Phe Leu Glu Gln Leu Met Glu

340 345 350

Ser Gly Ile Val Asn Asp Gly Val Leu Ala Gln Asp Glu Thr Gln Ile

355 360 365

Gly Gln Leu Trp Ser Leu Arg Glu Gly Ile Pro Glu Ala Ala Gly Lys

370 375 380

Ala Gly Arg Val Tyr Lys Tyr Asp Leu Ser Leu Pro Val Glu Lys Met

385 390 395 400

Tyr Ser Leu Val Pro Glu Leu Arg Gln Lys Leu Ala Glu Lys Gly Leu

405 410 415

Leu Ala Ala Glu Ser Glu Gly Gly Asn Gly Asp Gly Pro Val Lys Thr

420 425 430

Val Phe Gly Phe Gly His Leu Gly Asp Gly Asn Leu His Ile Asn Ile

435 440 445

Val Ala Asp Ala Tyr Arg Lys Glu Val Glu Glu Val Val Glu Pro Tyr

450 455 460

Ile Tyr Glu Leu Val Ala Lys Tyr Asn Gly Ser Ile Ser Ala Glu His

465 470 475 480

Gly Leu Gly Leu Met Lys Ala Pro Tyr Val Ala Tyr Ser Gln Asp Ala

485 490 495

Pro Ser Leu Asp Leu Met Arg Thr Leu Lys Lys Thr Leu Asp Pro Lys

500 505 510

Gly Ile Leu Asn Pro Tyr Lys Cys Val Thr Ala Glu

515 520

<210> 186

<211> 185

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 186

Met Ala Leu Tyr Tyr Gly Ile Val Phe Gly Ile Leu Thr Phe Glu Ile

1 5 10 15

Ile Leu Phe Phe Leu Phe Leu Leu Pro Ile Pro Thr Arg Trp Gln Lys

20 25 30

Pro Val Phe Arg Trp Leu Ala Thr Ser Pro Thr Ile Ala His Ala Gln

35 40 45

Tyr Ile Met Lys Ile Val Phe Val Phe Ile Phe Val Leu Phe Leu Asp

50 55 60

Ser Val Asn Thr Leu Arg Ala Phe Tyr Glu Val Val Asn Thr Glu Asp

65 70 75 80

Glu Asn Gly Gly Ile Pro Ala Ala Gly Asn Ser Asp Phe Arg Ala Gln

85 90 95

Val Gly Gln Ala Ala Lys Lys Phe Tyr Ala Gln Arg Asn Leu Tyr Leu

100 105 110

Thr Gly Phe Thr Ile Leu Leu Leu Leu Ile Leu Asn Lys Ile Lys Asn

115 120 125

Met Ala Met Asp Tyr Ile Arg Leu Glu Asp Gln Phe Ile Glu Leu Glu

130 135 140

Gly Ser Val Ser Lys Asp Pro Ala Ile Arg Lys Ala Ser Lys Glu Ile

145 150 155 160

Asp Thr Thr Pro Ile Glu Asp His Val Thr Arg Leu Glu Pro Val Glu

165 170 175

Gln Glu Gln Glu Asn Lys Lys Asp Ile

180 185

<210> 187

<211> 138

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 187

Val Ala Arg Arg Glu Ala Pro Asn Gly His Glu Leu Pro Pro Arg Gly

1 5 10 15

Tyr Asp Pro Gly Glu Asn Thr Tyr Gln Ala Pro Pro Asp Glu Arg Ser

20 25 30

Gln Val Asp Val Ala Ile Asp Pro Lys Ser Asn Arg Leu Gln Leu Leu

35 40 45

Lys Pro Phe Gln Lys Trp Asp Gly Lys Asp Ile Thr Asn Val Pro Ile

50 55 60

Leu Ile Lys Val Gln Gly Lys Cys Thr Thr Asp His Ile Ser Met Ala

65 70 75 80

Gly Pro Trp Leu Lys Tyr Arg Gly His Leu Asp Asn Ile Ser Asn Asn

85 90 95

Phe Leu Ile Gly Ala Lys Ser Ser Glu Gly Lys Val Asn Ser Ile Lys

100 105 110

Asn Ala Phe Thr Gly Glu Tyr Lys Gly Val Gln Lys Gln Leu Val Ile

115 120 125

Thr Arg Arg Lys Val Phe Val Gly Ser Trp

130 135

<210> 188

<211> 144

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 188

Gly Cys Pro Glu Thr Ala Arg Asp Tyr Lys Lys Glu Gly Val Arg Trp

1 5 10 15

Val Val Val Gly Asp Glu Asn Tyr Gly Glu Gly Ser Ser Arg Glu His

20 25 30

Ala Ala Leu Glu Pro Arg Phe Leu Asn Gly Ala Ala Ile Ile Thr Lys

35 40 45

Ser Phe Ala Arg Ile His Glu Thr Asn Leu Lys Lys Gln Gly Met Leu

50 55 60

Pro Leu Thr Phe Ala Asp Pro Lys Asp Tyr Asp Lys Val Asp Ala Ser

65 70 75 80

Asp Lys Val Asp Ile Leu Gly Leu Thr Asp Phe Gln Glu Gly Lys Pro

85 90 95

Leu Thr Leu Arg Leu His Lys Lys Asp Gly Ser Thr Val Asp Val Pro

100 105 110

Leu Asn His Thr Phe Asn Gly Gln Gln Ile Glu Trp Phe Lys His Gly

115 120 125

Ser Ala Leu Asn Leu Met Lys Glu Asn Thr Ala Lys Asn Gly Ser Leu

130 135 140

<210> 189

<211> 264

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 189

Val Ala Arg Arg Glu Ala Pro Asn Gly His Glu Leu Pro Pro Arg Gly

1 5 10 15

Tyr Asp Pro Gly Glu Asn Thr Tyr Gln Ala Pro Pro Asp Glu Arg Ser

20 25 30

Gln Val Asp Val Ala Ile Asp Pro Lys Ser Asn Arg Leu Gln Leu Leu

35 40 45

Lys Pro Phe Gln Lys Trp Asp Gly Lys Asp Ile Thr Asn Val Pro Ile

50 55 60

Leu Ile Lys Val Gln Gly Lys Cys Thr Thr Asp His Ile Ser Met Ala

65 70 75 80

Gly Pro Trp Leu Lys Tyr Arg Gly His Leu Asp Asn Ile Ser Asn Asn

85 90 95

Phe Leu Ile Gly Ala Lys Ser Ser Glu Gly Lys Val Asn Ser Ile Lys

100 105 110

Asn Ala Phe Thr Gly Glu Tyr Lys Gly Val Pro Glu Thr Ala Arg Asp

115 120 125

Tyr Lys Lys Glu Gly Val Arg Trp Val Val Val Gly Asp Glu Asn Tyr

130 135 140

Gly Glu Gly Ser Ser Arg Glu His Ala Ala Leu Glu Pro Arg Phe Leu

145 150 155 160

Asn Gly Ala Ala Ile Ile Thr Lys Ser Phe Ala Arg Ile His Glu Thr

165 170 175

Asn Leu Lys Lys Gln Gly Met Leu Pro Leu Thr Phe Ala Asp Pro Lys

180 185 190

Asp Tyr Asp Lys Val Asp Ala Ser Asp Lys Val Asp Ile Leu Gly Leu

195 200 205

Thr Asp Phe Gln Glu Gly Lys Pro Leu Thr Leu Arg Leu His Lys Lys

210 215 220

Asp Gly Ser Thr Val Asp Val Pro Leu Asn His Thr Phe Asn Gly Gln

225 230 235 240

Gln Ile Glu Trp Phe Lys His Gly Ser Ala Leu Asn Leu Met Lys Glu

245 250 255

Asn Thr Ala Lys Asn Gly Ser Leu

260

<210> 190

<211> 195

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 190

Gly Ala Pro Leu Thr Gln Glu His Gly Phe Pro Val Arg Val Ile Val

1 5 10 15

Pro Gly Val Ala Gly Ala Arg Ala Val Lys Trp Leu Asp His Ile Thr

20 25 30

Val Gln Arg Glu Met Ser Ser Asn His Tyr Met His Phe Asp Tyr Lys

35 40 45

Val Leu Pro Pro Glu Ala Val Asp Ala Glu Arg Ala Arg Thr Phe Trp

50 55 60

His Lys Val Pro Pro Val Ile Asp Met Pro Ala Asn Ser Ala Ile Thr

65 70 75 80

Ser Pro Arg Asn Glu Asp Thr Val Glu Val Asp Ala Glu Gly Phe Ile

85 90 95

Thr Val Asp Gly Tyr Ala Leu Pro Gly Gly Glu Asp Gly Pro Val Lys

100 105 110

Arg Val Glu Val Ser Ile Asp Lys Glu Arg Trp Val Asp Ala Glu Leu

115 120 125

Phe Thr His Pro Met Glu Ser Lys Trp Thr Trp Lys Ile Trp Lys Ala

130 135 140

Lys Val Gln Val Glu Pro Gly Glu Arg Arg Cys Leu Tyr Ser Arg Thr

145 150 155 160

Thr Asp Glu Ala Gly Asn Ser Gln Pro Gln Arg Ser Gln Trp Asn Leu

165 170 175

Arg Gly Val Cys Tyr Asn Gly Tyr Gly Glu Val Arg Asn Leu Lys Val

180 185 190

Val Lys Gly

195

<210> 191

<211> 50

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 191

Met Pro Ala Asn Thr Met Ser Ala Thr Leu Arg Ser Leu His Val Pro

1 5 10 15

Gly Lys Pro Val Ile Phe Ala Asn Val Trp Asp Thr Val Ser Ala Lys

20 25 30

Ser Ile Ala Pro Leu Asp Ser Cys Lys Ala Leu Ala Thr Ala Ser Tyr

35 40 45

Ala Ile

50

<210> 192

<211> 373

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 192

Ala Leu Pro Met Leu Leu Ile Asn Pro Pro Arg Asn Leu Ile Gly Gln

1 5 10 15

Ser Gln Ser Gly Thr Gly Lys Thr Ala Ala Phe Thr Leu Asn Met Leu

20 25 30

Ser Arg Val Asp Pro Asn Ile Met Thr Pro Gln Ala Ile Cys Leu Ala

35 40 45

Pro Ser Arg Glu Leu Ala Arg Gln Ile Gln Glu Val Val Asp Lys Ile

50 55 60

Gly Gln Phe Thr Gln Ile Lys Ser Phe Leu Ala Val Pro Gly Ser Trp

65 70 75 80

Ser Arg Asn Val Lys Ile Asp Lys His Ile Leu Val Gly Thr Pro Gly

85 90 95

Thr Leu Val Asp Met Leu Ser Arg Gly Gly Arg Ile Phe Asp Pro Lys

100 105 110

Gln Ile Arg Val Phe Val Leu Asp Glu Ala Asp Glu Met Ile Ala Leu

115 120 125

Gln Gly Leu Gly Asp Gln Thr Lys Arg Ile Lys Arg Met Leu Pro Pro

130 135 140

Gly Val Gln Asn Val Leu Phe Ser Ala Thr Phe Pro Asp Asn Val Arg

145 150 155 160

Asp Phe Ala Gly Asp Phe Ala Pro Glu Ala Asn Gln Ile Phe Leu Lys

165 170 175

Lys Glu Glu Ile Thr Val Asp Ala Ile Lys Gln Leu Tyr Leu Glu Cys

180 185 190

Asp Gly Glu Glu Gln Lys Tyr Asn Ala Leu Ser Ala Leu Tyr Asp Ile

195 200 205

Met Ser Ile Gly Gln Ser Ile Val Phe Cys Lys Arg Lys Asp Thr Ala

210 215 220

Asp Arg Ile Ala Ala Arg Leu Thr Asp Glu Gly His Ser Val Ala Ser

225 230 235 240

Leu His Gly Asp Lys Gln Thr Arg Asp Arg Asp Asp Ile Leu Asp Ala

245 250 255

Phe Arg Asp Gly Lys Thr Lys Val Leu Ile Thr Thr Asn Val Val Ala

260 265 270

Arg Gly Ile Asp Ile Gln Gln Val Asn Met Val Val Asn Tyr Asp Val

275 280 285

Pro Asp Leu Gly Pro Glu Gly Asp Trp Lys Pro Asp Ile Glu Thr Tyr

290 295 300

Ile His Arg Ile Gly Arg Thr Gly Arg Phe Gly Arg Lys Gly Cys Ser

305 310 315 320

Val Ile Phe Ala His Asp Gln Arg Ser Met Gln Asp Val Gln Phe Ile

325 330 335

Ala Asp Thr Leu Gly Lys Lys Met Ser Arg Ile Asn Ala Thr Arg Gln

340 345 350

Thr Asp Leu Asp Gln Leu Glu Ala Ala Leu Lys Ala Ala Ile Lys Gly

355 360 365

Asn Gln Pro Lys Glu

370

<210> 193

<211> 274

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 193

Met Ala Thr Phe Ser Thr Arg Ile Asn Leu Val Pro Thr Ser Arg Thr

1 5 10 15

Leu Ala Ser Gly Val Pro Phe Ala Pro Arg Ile Ala Leu Val His Pro

20 25 30

Pro Ala Ser His Gly His Gly Thr Ser Gly Pro Arg Ser Asp Val Pro

35 40 45

Pro Arg Trp Ala Gly Val Gln Gly Gly Phe Ala Ser Asn Ser Arg Val

50 55 60

Asn Val Leu Pro Thr Gly Asn Phe Gln Gln Arg Phe Met Ser Thr Thr

65 70 75 80

Pro Ala Arg Lys Ile Glu Ala Gln Pro His Val Arg Gly Val Pro Asp

85 90 95

Trp Ser Ala Tyr Gln Ser Ser Gly Lys Gly Glu Asn Thr Arg Ser Leu

100 105 110

Ser Tyr Phe Met Val Gly Ser Leu Gly Val Leu Ala Ala Ser Gly Ala

115 120 125

Lys Ser Thr Val Ser Asp Ile Leu Ser Asn Met Ala Ala Ser Ala Asp

130 135 140

Val Leu Ala Leu Ala Lys Ile Glu Val Glu Met Gly Ala Ile Pro Glu

145 150 155 160

Gly Lys Asn Leu Ile Val Lys Trp Arg Gly Lys Pro Val Phe Ile Arg

165 170 175

His Arg Thr Glu Asp Glu Ile Asn Glu Ala Arg Ala Val Asp Ile Lys

180 185 190

Ser Leu Arg Asp Pro Glu Ser Asp Glu Asp Arg Thr Gln Arg Gly Glu

195 200 205

Trp Leu Val Met Leu Gly Val Cys Thr His Leu Gly Cys Val Pro Ile

210 215 220

Gly Glu Ala Gly Asp Tyr Gly Gly Trp Phe Cys Pro Cys His Gly Ser

225 230 235 240

His Tyr Asp Ile Ser Gly Arg Ile Arg Arg Gly Pro Ala Pro Leu Asn

245 250 255

Leu Glu Val Pro Glu Tyr Ala Phe Asn Asp Asp Glu Glu Lys Leu Val

260 265 270

Ile Gly

<210> 194

<211> 472

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 194

Gly Glu His His His Ser Asp Arg Asp Cys Ser Ser Val Lys Arg Val

1 5 10 15

Phe Ala Leu Gly Arg Leu Asp Val Thr Leu Ala Val Glu Cys Gly Ser

20 25 30

Val Arg Thr Met Ser Leu Arg Asp Leu Ala Cys Tyr Thr Leu Thr Leu

35 40 45

Lys Pro Ser Thr Glu Asn Thr Leu Leu Thr Glu Leu Thr Ala Leu Glu

50 55 60

Gly Pro Ser Glu Glu Pro Arg Phe Ala Arg Val Arg Glu Lys Val Glu

65 70 75 80

Gly Glu Val Tyr Ser Ser Ala Ile Tyr Asp Ala Leu Thr Gly Ala Lys

85 90 95

Leu Ala Ser Val Gly Phe Ala Ser Glu Lys Gln Lys Asn Arg Arg Leu

100 105 110

Gln Leu His Asn Pro Asp Glu Ser Val Pro Phe Asp Asn Thr Ser Lys

115 120 125

Leu Gly Phe Glu Trp Thr Phe Ile Phe Glu Gly Asn Lys Tyr Arg Trp

130 135 140

Thr Arg Glu Leu Tyr Gly Lys Asp Tyr Ile Cys Ser Leu Asp Arg Lys

145 150 155 160

Pro Asp Pro Arg Val Glu Ile Cys Leu Ala Arg Asp Ala Asp Ser Lys

165 170 175

Ala Pro Gly Arg Leu Gln Ile Leu His Tyr Asn Ile Glu Arg Phe Pro

180 185 190

Asn Glu Ile Lys Asp Leu Arg Gly Leu Glu Thr Leu Leu Ile Ala Thr

195 200 205

Leu Met Cys Phe Val Asp Ala Ala Glu Asp Arg Ser Asn Ser Gly Pro

210 215 220

Thr Arg Thr Ser Pro Leu Pro Ala Lys Pro Val Ala Asn Ala Ala Ala

225 230 235 240

Gly Gln Ser Gly Thr Ser Ala Ser Gly Ser Ser Asp Thr Arg Ala Lys

245 250 255

Val Ala Pro Val Thr Val Pro Val Ile Thr Ala Glu Asp Phe Glu Asp

260 265 270

Asp Cys Asp Pro Asn Glu Ile Leu Val Gly Thr Glu Thr Asp Val Gly

275 280 285

Glu His Ile Ala Arg Ala Ile Ala Leu Leu Glu Asp Pro Thr Met Leu

290 295 300

Phe Ile Val Ile Arg Thr Arg Thr Ala Ala Ala Ser Ser Arg Ala Leu

305 310 315 320

Glu Val Ser Leu Gly Val Thr Arg Phe Arg His Arg Glu Gly Met Ser

325 330 335

Glu Leu His Gln Tyr Val Val Glu Glu Asp Pro Val Arg Lys Pro Lys

340 345 350

Pro Ile Met Pro Ala Gln Gly Leu Lys Leu Ile Asn Leu Asp Asp Arg

355 360 365

Pro Ala Ala Gln Ser Pro Thr Lys Pro Glu Trp Ser Ala Pro Pro Asn

370 375 380

Ile Ala Val Tyr Leu Ser Ser Ile Glu Leu Pro Asp Leu Thr Pro Lys

385 390 395 400

Pro Lys Pro Val Gln Gly His Thr Arg Pro Pro Thr Gln Ala Pro His

405 410 415

Ala Arg Pro Pro Pro Pro Ser Gln Leu Pro Gln Lys Pro Gln Pro Arg

420 425 430

Pro Arg Pro Pro Pro Ser Asp Gly Ser Gly Ser Ser Gln Thr Thr Leu

435 440 445

Ala Ser Thr Arg Pro Pro Gln Asp Asp Gly Lys Asp Ser Arg Lys Ser

450 455 460

Ser Phe Gly Arg Leu Phe Gly Arg

465 470

<210> 195

<211> 82

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 195

Met Ala Ser Gln Leu Met Pro Leu Glu Leu Ile Asp Arg Cys Ile Gly

1 5 10 15

Ser Arg Met Arg Val Ile Met Lys Gly Asp Lys Glu Phe Ser Gly Thr

20 25 30

Leu Leu Gly Phe Asp Asp Phe Val Asn Met Val Leu Glu Asp Val Thr

35 40 45

Glu Tyr Asp Tyr Thr Gly Ala Thr Thr Lys Leu Pro Lys Ile Leu Leu

50 55 60

Asn Gly Asn Asn Ile Cys Met Leu Ile Pro Gly Gly Met Pro Glu Gly

65 70 75 80

Glu Ser

<210> 196

<211> 116

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 196

Gly Asp Asp Asn Lys Lys Thr Ile Pro His Leu Asn Ile Phe Asn Asn

1 5 10 15

Gly Val Pro Ile Asp Ala Pro Gly Ala Asp Arg Ser Leu His Arg Ile

20 25 30

Lys Asn Ala Cys Asp His Glu Arg Arg Gln Arg Val Gln Arg His Thr

35 40 45

Ser Arg Ile Arg Arg Leu Arg Gln Tyr Gly Ala Arg Gly Cys His Arg

50 55 60

Val Arg Leu His Arg Arg Asn Asp Gln Ala Ser Gln Asp Pro Ser Glu

65 70 75 80

Arg Gln Gln His Leu His Ala His Pro Arg Trp His Ala Arg Gly Arg

85 90 95

Val Met Asn His Gly His Met Ile Ser Leu Leu Thr Ser Leu Glu Met

100 105 110

Ala Lys Arg Val

115

<210> 197

<211> 108

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 197

Ala Lys Glu Leu Ser Pro Asp Val Lys Pro Glu Pro Thr Trp Ser Cys

1 5 10 15

Gly Glu Val Val Asn Val Val Asp Glu His Gly Asn Val Ile Lys Pro

20 25 30

Ser Asp Leu Trp Val Lys Met Gly Met Gln Gln Gln Asp Asn Val Asp

35 40 45

Asn Leu Leu Ile Asp Asp Leu Cys Asp Gln Met Arg Ala Lys Ala Lys

50 55 60

Cys Thr Glu Asn Gly Ala Gln Leu Asn Val Asp Asp Leu Asn His Met

65 70 75 80

Met Ser Tyr Asp Lys Ser Tyr Lys Gln Lys Arg Val Asp Asp Leu Lys

85 90 95

Asp Lys Tyr Gly Trp Gly Ala Val Phe Gly Pro Lys

100 105

<210> 198

<211> 38

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 198

Gly Ser Leu Thr Arg Arg Ala Trp Phe Ile Gln Ser Thr Cys Ala Thr

1 5 10 15

Thr Gly Asp Gly Leu Tyr Glu Gly Leu Glu Trp Leu Ala Asp Thr Leu

20 25 30

Arg Lys Thr Asn Arg Asp

35

<210> 199

<211> 311

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 199

Met Glu Asn Leu Leu Arg Gln Met Gln Gly Gly Gly Gly Arg Met Gly

1 5 10 15

Ala Arg Pro Gly Pro Gly Gly Glu Thr Ile Leu Ala Asp Asn Gly Glu

20 25 30

Thr Val His Ile Ser Ser Leu Ala Leu Leu Lys Met Leu Lys His Gly

35 40 45

Arg Ala Gly Val Pro Met Glu Val Met Gly Leu Met Leu Gly Glu Phe

50 55 60

Val Asp Asp Tyr Thr Ile Ser Cys Val Asp Val Phe Ala Met Pro Gln

65 70 75 80

Ser Gly Thr Thr Val Thr Val Glu Ser Val Asp His Val Phe Gln Thr

85 90 95

Lys Met Leu Asp Met Leu Lys Gln Thr Gly Arg Pro Glu Met Val Val

100 105 110

Gly Trp Tyr His Ser His Pro Gly Phe Gly Cys Trp Leu Ser Ser Val

115 120 125

Asp Val Asn Thr Gln Gln Ser Phe Glu Gln Leu His Pro Arg Ala Val

130 135 140

Ala Val Val Ile Asp Pro Ile Gln Ser Val Arg Gly Lys Val Val Ile

145 150 155 160

Asp Ala Phe Arg Ser Ile Asn Pro Gln Ser Leu Val Ala Gly Gln Glu

165 170 175

Ser Arg Gln Thr Thr Ser Asn Ile Gly His Leu Asn Lys Pro Ser Ile

180 185 190

Gln Ala Leu Ile His Gly Leu Asn Arg His Tyr Tyr Ser Leu Ala Ile

195 200 205

Asp Tyr Arg Lys Thr Glu Gly Glu Gln Gly Met Leu Leu Asn Leu His

210 215 220

Lys Arg Gly Trp Thr Glu Gly Leu Lys Met Arg Asp His Ser Glu Met

225 230 235 240

Lys Glu Gly Asn Glu Lys Ala Ile Lys Glu Met Leu Ser Leu Ala Ser

245 250 255

Ala Tyr Thr Lys Ser Val Gln Glu Glu Thr Thr Met Thr Ala Glu Gln

260 265 270

Leu Lys Thr Arg His Val Gly Lys Leu Asp Pro Lys Arg His Leu Gly

275 280 285

Glu Ala Ala Glu Lys Ala Met Gly Asp Gln Val Thr Gln Ser Leu Ala

290 295 300

Met Gly Val Leu Ala Glu Leu

305 310

<210> 200

<211> 213

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 200

Gly His Thr Gly Asp Val Leu Ser Val Ser Phe Ser Ala Asp Asn Arg

1 5 10 15

Gln Ile Val Ser Ala Ser Arg Asp Arg Thr Thr Lys Leu Trp Asn Thr

20 25 30

Leu Gly Glu Cys Lys Phe Asn Ile Val Asp Asp Gly His Ser Glu Trp

35 40 45

Val Ser Cys Val Arg Phe Ser Pro Asn Pro Val Ile Pro Val Ile Val

50 55 60

Ser Ala Gly Trp Asp Lys Val Val Lys Val Trp Glu Leu Ser Lys Cys

65 70 75 80

Lys Leu Lys Thr Asn His His Gly His Thr Gly Tyr Ile Asn Thr Leu

85 90 95

Ala Val Ser Pro Asp Gly Ser Leu Ala Ala Ser Gly Gly Lys Tyr Gly

100 105 110

Ile Thr Met Leu Trp Asp Leu Asn Asp Gly Lys His Leu Tyr Ser Leu

115 120 125

Glu Ala Gly Asp Ile Val Asn Ser Leu Val Phe Ser Pro Asn Arg Tyr

130 135 140

Trp Leu Cys Ala Ala Thr Ala Ser Ser Ile Lys Ile Leu Asp Leu Glu

145 150 155 160

Ser Lys Ser Ile Val Asp Asp Leu Lys Pro Asp Phe Ser Ala Glu Tyr

165 170 175

Pro Asp Lys Ala Gln Lys Pro Gln Cys Thr Ser Leu Ala Trp Ser Ala

180 185 190

Asp Gly Gln Thr Leu Phe Ala Gly Phe Ser Asp Asn Leu Val Arg Val

195 200 205

Trp Val Val Thr Ala

210

<210> 201

<211> 76

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 201

Met Val Thr Arg Ser Gly Ser Leu Ala Phe Asp Ser Leu Leu Thr Pro

1 5 10 15

Ser Phe Pro Ser Ser Ser Leu Leu Val Gly Thr Arg Ser Ser Arg Ser

20 25 30

Gly Asn Cys Pro Ser Ala Ser Ser Arg Pro Thr Thr Thr Val Thr Leu

35 40 45

Val Thr Ser Thr Pro Ser Pro Phe Arg Pro Thr Asp Arg Ser Pro His

50 55 60

Pro Val Glu Ser Met Ala Ser Pro Cys Phe Gly Ile

65 70 75

<210> 202

<211> 99

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 202

Met Leu Trp Asp Leu Asn Asp Gly Lys His Leu Tyr Ser Leu Glu Ala

1 5 10 15

Gly Asp Ile Val Asn Ser Leu Val Phe Ser Pro Asn Arg Tyr Trp Leu

20 25 30

Cys Ala Ala Thr Ala Ser Ser Ile Lys Ile Leu Asp Leu Glu Ser Lys

35 40 45

Ser Ile Val Asp Asp Leu Lys Pro Asp Phe Ser Ala Glu Tyr Pro Asp

50 55 60

Lys Ala Gln Lys Pro Gln Cys Thr Ser Leu Ala Trp Ser Ala Asp Gly

65 70 75 80

Gln Thr Leu Phe Ala Gly Phe Ser Asp Asn Leu Val Arg Val Trp Val

85 90 95

Val Thr Ala

<210> 203

<211> 124

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 203

Val Phe Ala Ile Val Gln Ile Pro Lys His Gly Asp Ala Ile Leu Ser

1 5 10 15

Thr Gly Cys Gly Glu Arg Ser Val Gly Arg Asn Gly Glu Gly Val Asp

20 25 30

Val Thr Ser Val Thr Val Val Val Gly Leu Glu Leu Ala Leu Gly Gln

35 40 45

Phe Pro Asp Leu Asp Asp Leu Val Pro Thr Ser Arg Asp Asp Asp Gly

50 55 60

Asn Asp Gly Val Arg Arg Glu Ser Asn Ala Arg Asp Pro Leu Arg Val

65 70 75 80

Thr Ile Val Asn Asn Val Glu Leu Ala Leu Ser Glu Ser Val Pro Glu

85 90 95

Leu Gly Ser Ser Val Ser Gly Ser Arg Asn Asp Leu Ser Val Val Gly

100 105 110

Arg Glu Arg Asp Ala Gln Asp Val Thr Gly Val Ser

115 120

<210> 204

<211> 38

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 204

Gly Ser Leu Thr Arg Arg Ala Trp Phe Ile Gln Ser Thr Cys Ala Thr

1 5 10 15

Thr Gly Asp Gly Leu Tyr Glu Gly Leu Glu Trp Leu Ala Asp Thr Leu

20 25 30

Arg Lys Thr Asn Arg Asp

35

<210> 205

<211> 393

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 205

Gly Arg Tyr Asp Phe Lys Gln Pro Gln Arg Ile Arg Asp Ala Ser Val

1 5 10 15

Thr Ala Thr Pro Glu Trp Asn Leu Leu Glu Glu Ile Glu Phe Gly Arg

20 25 30

Leu Gly Lys Leu Asn Leu Ser Val Glu Glu Pro Glu Asp Leu Glu Ser

35 40 45

His Gly Thr Leu Gln Gly Tyr Asp Lys Thr Phe Asp Arg Ile Asn Thr

50 55 60

Arg Thr Glu Arg Pro Leu Glu Ile Ile Asp Arg Ala Trp Tyr Asn Gln

65 70 75 80

Thr Thr Ser Asp Asp Pro Val Ile Ala Gln Leu Ala Gln Thr Gln Ser

85 90 95

Ala Gln Ile Phe Ala Thr Asp Ala Ile Leu Ala Val Leu Met Cys Thr

100 105 110

Thr Arg Ser Val Asn Ser Trp Asp Ile Ile Leu Glu Arg Arg Gly Asn

115 120 125

Gln Leu Phe Leu Asp Lys Arg Asp Ser Gly Pro Phe Asp Tyr Val Thr

130 135 140

Val His Glu Asn Ala Ala Asp Pro Pro Ala Asp Ser Asp Asp Pro Asn

145 150 155 160

Asn Val Asn Ser Ala Ser Ser Leu Ser Leu Glu Ala Thr Tyr Ile Thr

165 170 175

Arg Asn Phe Ser Ser Gln Val Ile Asp Ala Lys Ser Lys Pro Tyr Ser

180 185 190

Pro Ser Pro Asn Pro Phe Tyr Ser Glu Asp Glu Pro Ser Pro Val Ala

195 200 205

Ser Cys Leu Tyr Arg Tyr Arg Lys Phe Asp Leu Ser Val Gly Glu Glu

210 215 220

Asp Thr Leu Asp Leu Ile Val Arg Thr Glu Val Asp Ala Tyr Gln Gly

225 230 235 240

Lys Lys Asp Ser Leu Val Thr Val Lys Ala Leu Asn Glu Phe Asp Pro

245 250 255

Arg Ala Ser Gly Gly Gly Lys Ala Leu Asp Trp Arg Lys Tyr Leu Asp

260 265 270

Thr Gln Lys Gly Ala Ile Val Ala Ser Glu Met Lys Asn Asn Ser Ala

275 280 285

Lys Leu Ala Arg Trp Ala Ile Gln Ser Val Leu Ala Gly Ala Glu Val

290 295 300

Met Lys Met Gly Tyr Ile Ser Arg Ala Ser Pro Arg Asp Thr Thr His

305 310 315 320

His Val Ile Val Gly Val Gln Asn Tyr Lys Pro Lys Asp Phe Ala Ala

325 330 335

Gln Met Asn Val Ser Leu Asn Asn Gly Trp Gly Ile Val Arg Thr Ile

340 345 350

Ala Asp Leu Val Leu Lys Gln Pro Glu Gly Lys Tyr Val Leu Val Lys

355 360 365

Asp Pro Asn Ala Gly Ile Ile Arg Leu Tyr Ser Val Pro Glu Asn Ala

370 375 380

Phe Glu Ala Glu Glu Glu Glu Glu Gln

385 390

<210> 206

<211> 106

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 206

Met Thr Ser Ser Ser Leu Ser Glu Phe Glu Thr Leu Leu Ser Arg Pro

1 5 10 15

Arg Gln Asn Gly Thr Cys Leu Lys Arg Ser Ser Leu Ala Asp Trp Ala

20 25 30

Ser Ser Thr Phe Pro Ser Lys Ser Pro Lys Thr Ser Asn Arg Thr Val

35 40 45

Pro Ser Lys Val Thr Thr Arg Arg Leu Thr Ala Ser Thr Leu Val Pro

50 55 60

Lys Asp Leu Ser Arg Ser Leu Ile Glu His Gly Thr Ile Lys Pro Leu

65 70 75 80

Leu Thr Ile Pro Leu Leu Leu Ser Ser Leu Lys Arg Ser Leu Pro Lys

85 90 95

Ser Ser Arg Gln Met Pro Phe Leu Arg Phe

100 105

<210> 207

<211> 245

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 207

Glu Arg Ser Arg Ser Leu Ala Pro Glu Ala Asp Gln Gly Val Ala Thr

1 5 10 15

Gln Cys His Glu Val Arg Ser Ser Gly Ser Tyr Asp Thr Phe Glu Ile

20 25 30

Gly His His Gln Pro Asp Ser Asp Thr Ser Gly Val Ala Asp Leu Arg

35 40 45

Thr Ser Ser Arg Met Asp Thr Cys Asp Ala His Leu Leu Arg Arg Val

50 55 60

Lys Ser Cys Pro Leu Phe Ser Tyr Arg Glu Asp Glu Val Ser Glu Thr

65 70 75 80

Val Gln Leu Pro Thr Gly Glu Trp Thr Thr Ile Arg Asp Ile Thr Pro

85 90 95

Ser Ala Pro Lys Ile Gly Phe Glu Val Arg Asp Ser Leu Ser Ala Phe

100 105 110

Pro Thr Ala Lys Pro Val Glu Ala Lys His Glu Ser Ala Ser Ser Ile

115 120 125

Ser Asn Asp Leu Pro Ser Gln Pro Ser Ser Arg Pro Leu Ile Glu Cys

130 135 140

Pro Thr Leu Val Ala Asp Ser Arg Thr Thr Thr Gly Ser Asn Ser Val

145 150 155 160

Arg Ser Phe Asp Ala Gln Thr Glu Arg Leu Ser Gly Leu Ser Asp Val

165 170 175

His His Arg Tyr Met Gln Asp Lys Pro Ser Gln Arg Ser Asp Ser Trp

180 185 190

Thr Asp Val Lys Ser Ser Ala Pro Ser Ser Gln Ser Met Ala Val Pro

195 200 205

Asn Lys Ala Ala Tyr Leu Ala Pro Ile Pro Ala Gly Pro Asn Asp Ser

210 215 220

Lys Thr Ser Ser Ser Gly Arg Ala Pro Ser Asp Ala Ala Thr Glu His

225 230 235 240

Glu Cys Ser Leu Gln

245

<210> 208

<211> 148

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 208

Met Ala Pro Lys Ser Thr Asp Lys Pro Ala Ser Thr Ala Gly Lys Ala

1 5 10 15

Pro Ser Ala Gly Gly Lys Ala Pro Ala Ser Lys Thr Val Gly Ala Lys

20 25 30

Lys Thr Ala Ala Lys Lys Ser Ala Lys Ser Thr Gly Glu Gly Gly Glu

35 40 45

Lys Lys Lys Arg Val Lys Ser Arg Lys Glu Thr Tyr Ser Thr Tyr Ile

50 55 60

Tyr Lys Val Leu Lys Gln Val His Pro Asp Thr Gly Ile Ser Asn Lys

65 70 75 80

Ala Met Leu Ile Leu Asn Ser Phe Val Asn Asp Ile Phe Glu Arg Ile

85 90 95

Ala Gly Glu Ala Ser Lys Leu Ala Thr Tyr Asn Lys Lys Ser Thr Ile

100 105 110

Ser Ser Arg Glu Ile Gln Thr Ala Val Arg Leu Ile Leu Pro Gly Glu

115 120 125

Leu Ser Lys His Ala Ile Ser Glu Gly Thr Lys Gly Val Thr Lys Tyr

130 135 140

Ser Ser Ser Lys

145

<210> 209

<211> 369

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 209

Asp Val Lys Arg Phe Thr Lys Asp Leu Leu Phe Asn Ser Glu Gly Asn

1 5 10 15

Leu Thr Phe Lys Pro His Leu Trp Asn Asp Ile Arg His Thr Leu Leu

20 25 30

Pro Thr Phe Ile Arg Gln Ile Gly Tyr Val Pro Ile Pro Arg Ala Glu

35 40 45

Phe Ser Ser Pro Asp Ile Asp Leu Val Ile Glu Asn Leu Val Leu Ser

50 55 60

Gly Pro Asn Leu Phe Pro Asn Val Val Ser Leu Glu Ser His Asn Ser

65 70 75 80

Phe Lys Phe Ser Pro Tyr Gln Gln Leu Asn Lys Gly Met Asp Thr His

85 90 95

His His Lys Phe Arg Leu Gly Met Ser Gln Ile Gln Ala Asp Ile Arg

100 105 110

Asp Val Arg Phe Ser Phe Arg Arg Lys Thr Gly Trp Pro Lys Leu Lys

115 120 125

Asp His Gly Leu Ala Asp Val Ile Leu Ala Gly Lys Gly Met Ser Ile

130 135 140

Asp Val Glu Leu Glu Ser Val Glu Gly Arg Arg Asp Ser Val Val Arg

145 150 155 160

Val Asn His Val His Thr Thr Ile Asp Thr Leu Thr Phe Ser Ile Arg

165 170 175

Asp Ser Lys His Asp Leu Leu Tyr Lys Phe Val Lys Ser Val Ala Thr

180 185 190

Gly Thr Ile Lys Lys Ala Ile Gln Ala Ala Val Asp Asn Ala Ile Arg

195 200 205

Thr Ala Val Gly His Leu Asp Asp Gln Leu Val Gln Val Arg Asn Thr

210 215 220

Val Asp Asp Ala Lys Lys Ser Asp Glu Thr Thr Arg Thr Gln Ala Leu

225 230 235 240

Lys Asp Leu Tyr Ser Lys Lys Ala Asp Thr Ala Gln Lys Lys Gln Ala

245 250 255

Glu Ser Lys Glu Gln Pro Gly Thr Phe Arg Ile Val Ala Asn Arg Asp

260 265 270

Ser Val Leu Asn Pro Asp Met Gly Gly Gly Lys Gly Ala Met Thr Asn

275 280 285

Lys Met Trp Lys Thr Glu Asp Leu Ala His Ser Gly Lys Glu Trp His

290 295 300

Ser Pro Ala Phe Asp Leu Leu Asp Ser Lys His Pro Ala Arg Thr Gly

305 310 315 320

Gln Thr His Pro Glu Ala Lys Glu Gly Ala Gly His Gly Asn Ser Leu

325 330 335

Ser Ser Lys Ala Gln Pro Gly Ala Asn Ala Ala Asp Gln Leu Lys Ala

340 345 350

Thr His Gly Gln Ser Glu Ala Glu Ala Ile Ala Gly Gln Lys Arg Gln

355 360 365

Gln

<210> 210

<211> 173

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 210

Met Ser Asp Ser Arg Ser Asp Glu Arg Leu Asp Gly Pro Ser Ser Arg

1 5 10 15

Thr Thr Val Ser Pro Met Ser Ser Leu Pro Val Arg Val Cys Arg Ser

20 25 30

Thr Ser Ser Ser Ser Leu Ser Arg Asp Asp Glu Thr Leu Leu Cys Glu

35 40 45

Ser Thr Thr Ser Thr Pro Pro Ser Thr Pro Ser Pro Ser Pro Ser Glu

50 55 60

Thr Pro Ser Thr Thr Cys Ser Thr Ser Ser Ser Ser Arg Trp Pro Arg

65 70 75 80

Val Arg Ser Arg Arg Gln Ser Arg Pro Pro Ser Thr Met Pro Ser Val

85 90 95

Arg Leu Ser Val Thr Ser Thr Thr Ser Ser Ser Arg Ser Glu Thr Pro

100 105 110

Ser Met Thr Pro Arg Ser Leu Thr Arg Pro Pro Glu Arg Lys Pro Ser

115 120 125

Arg Thr Cys Thr Arg Arg Arg Arg Thr Arg His Arg Arg Ser Arg Pro

130 135 140

Ser Pro Arg Ser Ser Leu Val Leu Ser Glu Ser Ser Pro Thr Glu Thr

145 150 155 160

Leu Phe Ser Thr Pro Thr Trp Ala Val Ala Arg Ala Pro

165 170

<210> 211

<211> 248

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 211

Met Tyr Thr Ser Ala Val Thr Leu Leu Ser Leu Val Leu Leu Leu Ala

1 5 10 15

Thr Ser Val Ile Ala Gln Glu Gln Ala Gly Arg Pro Gly Thr Gln Arg

20 25 30

Gly Gly Val Phe Phe Gly Cys Tyr Ala Asp Arg Pro Thr Gly Asn Ala

35 40 45

Asn Gln Pro Ile Thr Arg Val Ala Asn Ser Asp Thr Phe Phe Glu Cys

50 55 60

Met Glu Asn Cys Ala Ala Ile Thr Ser Pro Ser Leu Leu Gly Tyr Tyr

65 70 75 80

Gln Pro Ser Ser Gly Gln Cys Phe Cys Gly Asn Leu Leu Phe Asn Pro

85 90 95

Gln Ala Gln Leu Asn Gly Asn Gly Cys Gln Gly Ser Asp Trp Ser Phe

100 105 110

Gly Arg Thr Ser Thr Thr Phe Arg Arg Phe Gly Asp Ala Cys Arg Pro

115 120 125

Phe Gly Gly Val Gly Phe Ser Ala Asn Gln Tyr Thr Thr Val Thr Gly

130 135 140

Pro Val Ala Cys His Val Gln Cys Ala Ser Asn Arg Phe Ala Tyr Val

145 150 155 160

Trp Ser Asp Thr Gly Ser Asn Ser Trp Gln Cys Ala Cys Ser Asn Asn

165 170 175

Val Arg Val Gln Glu Asp Phe Gln Tyr Thr Cys Gln Gly Gly Gly Val

180 185 190

Phe Val Phe Glu His Ser Val Gln Ala Gln Ala Ser Ser Leu Asn Arg

195 200 205

Lys Arg Thr Val Glu Glu Gln Trp Ala Val Pro Lys Asp Ala Leu Cys

210 215 220

Pro Phe Gly Met Ser Ala Cys Lys Val Ser Gly Val Asp Asn Ala Tyr

225 230 235 240

Glu Val Cys Phe Phe Ser Asp Arg

245

<210> 212

<211> 116

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 212

Met Phe Asn Ala His Gln Thr Asp Ser Pro Met Ser Gly Pro Ile Leu

1 5 10 15

Glu Ala Thr His Gly Asn Val Leu Ala Ala Thr Met Ser Val Phe Arg

20 25 30

Arg Thr Ser Ser Thr Leu Val Lys Val Ala Val Tyr Leu Cys Leu Asn

35 40 45

Ile Gln Tyr Lys Leu Arg Leu Leu Arg Leu Thr Gly Ser Gly Arg Trp

50 55 60

Arg Asn Asn Gly Leu Phe Arg Lys Thr Pro Ser Val His Ser Glu Cys

65 70 75 80

Gln Arg Ala Arg Tyr Gln Val Ser Ile Met Leu Thr Arg Tyr Ala Ser

85 90 95

Phe Gln Thr Ala Arg Pro Leu Val Pro Trp Pro Arg Gly Leu Lys His

100 105 110

Ala Ile Asp Leu

115

<210> 213

<211> 271

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 213

Gly Gly Gly Thr Val Val Ser Asn Ala Leu Leu Glu Asn Ala Lys Leu

1 5 10 15

Cys Lys Thr Gln Gly Lys Glu Ser Ser Leu Arg Val Ile Val Cys Gly

20 25 30

Arg Asn Arg Leu Glu Asn Gly Ser Ala Pro His Trp Ala Glu Ala Phe

35 40 45

Ala Thr His Gly Lys Leu Val Glu Val Arg Met Pro Gln Asn Gly Ile

50 55 60

Arg Met Glu Gly Ile Lys Ala Ile Ala Asp Gly Leu Ala Lys Cys Pro

65 70 75 80

Thr Leu Glu Val Leu Asp Leu Gln Asp Asn Thr Ala Thr Lys Thr Gly

85 90 95

Thr Arg Ser Ile Val Arg His Leu Ser Thr Trp Pro Lys Leu Arg Ile

100 105 110

Leu Asn Leu Ser Asp Cys Leu Leu Gly Ser Val Gly Gly Ile Ala Leu

115 120 125

Ala Thr Ala Leu Ser Thr Gly Ser Asn Lys His Leu Glu Gln Leu Lys

130 135 140

Leu Gln Tyr Gly Glu Phe Asp Lys Arg Thr Val Glu Ile Leu Ser Thr

145 150 155 160

Ala Ile Ser Gln His Leu Pro Lys Leu Thr Thr Leu Glu Leu Asn Gly

165 170 175

Asn Arg Phe Asp Ala Glu Asp Glu Cys Val Glu Thr Leu Lys Lys Ala

180 185 190

Leu Glu Leu His Gly Asn Glu Asp Ala Leu Asp Glu Leu Asp Asp Met

195 200 205

Glu Glu Val Asp Glu Asp Glu Glu Asp Asp Asp Asp Glu Asp Glu Glu

210 215 220

Asp Glu Asp Glu Asp Lys Asp Thr Ser Ala Asp Asp Gly Ile Asp Ala

225 230 235 240

Gly Ala Ala Gly Glu Asp Ala Leu Pro Pro Val Thr Lys Lys Asp Glu

245 250 255

Asp Val Leu Ala Asp Leu Leu Ser Lys Val His Val Gln Pro Ser

260 265 270

<210> 214

<211> 222

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 214

Leu Asp Arg Arg Ser Ala Ser Thr Ser Ser Ser Phe Phe Val Thr Gly

1 5 10 15

Gly Arg Ala Ser Ser Pro Ala Ala Pro Ala Ser Ile Pro Ser Ser Ala

20 25 30

Leu Val Ser Leu Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser Ser

35 40 45

Ser Ser Ser Ser Ser Ser Thr Ser Ser Ile Ser Ser Ser Ser Ser Lys

50 55 60

Ala Ser Ser Phe Pro Cys Ser Ser Ser Ala Phe Phe Arg Val Ser Thr

65 70 75 80

His Ser Ser Ser Ala Ser Lys Arg Phe Pro Phe Ser Ser Ser Val Val

85 90 95

Asn Phe Gly Lys Cys Trp Leu Ile Ala Val Asp Ser Ile Ser Thr Val

100 105 110

Leu Leu Ser Asn Ser Pro Tyr Cys Ser Leu Ser Cys Ser Arg Cys Leu

115 120 125

Phe Glu Pro Val Asp Asn Ala Val Ala Arg Ala Ile Pro Pro Thr Glu

130 135 140

Pro Lys Arg Gln Ser Glu Arg Leu Ser Ile Arg Ser Leu Gly Gln Val

145 150 155 160

Glu Arg Cys Arg Thr Ile Leu Arg Val Pro Val Leu Val Ala Val Leu

165 170 175

Ser Cys Lys Ser Ser Thr Ser Asn Val Gly His Leu Ala Ser Pro Ser

180 185 190

Ala Ile Ala Leu Met Pro Ser Met Arg Met Pro Phe Cys Gly Ile Leu

195 200 205

Thr Ser Thr Asn Leu Pro Cys Val Ala Asn Ala Ser Ala Gln

210 215 220

<210> 215

<211> 176

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 215

Met Val Lys Leu Ser Asn Ser Leu Val Arg Arg Leu Lys Trp Gln His

1 5 10 15

Val Arg Ser Leu Gly Val Val Ala Leu Thr Ala Gln Leu Arg Gly Pro

20 25 30

Gln Pro Gln Ser Ala Glu Asp Glu Asp Ser Glu Ala Ala Gly Lys Lys

35 40 45

Leu Lys Leu Ala Gly Asp Gln Ala Thr Ser Ala Val Ile Pro Lys Ser

50 55 60

Ala Asp Lys Pro Asp Thr Phe Pro Leu Leu Asp Thr Leu Pro Ala Thr

65 70 75 80

Met Ala Ala Gly Thr Arg Ser Met Thr Arg Pro Leu His Val Gly Asp

85 90 95

Leu Arg Leu Ala Asp Leu Arg Lys Ile Met Gln Ala Ala Gly His Thr

100 105 110

Ala Glu Phe Arg Gly Glu Gly Thr Leu Leu Ile Asp Lys Ser Val Ala

115 120 125

Val Arg Lys Ser Gly Thr Gly Gln Ile Glu Ile Glu Ala Ser Ala Gln

130 135 140

Ala Ala Ala Asn Gln Ala Thr Pro Gly Arg Gly Ala Ser Ser Phe Leu

145 150 155 160

Ala Val Lys Arg Lys Ile Tyr Glu Gly Leu Ala Val Val Thr Gly Ser

165 170 175

<210> 216

<211> 139

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 216

Lys Met Lys Ile Asp Val Glu Lys Leu Asn Lys Asp Ile Ser Leu Phe

1 5 10 15

Pro Gln Val His Pro Ile Thr Glu Asp Met Lys Ile Thr His Lys Gly

20 25 30

Val Ser Arg Leu Val Met Leu Asp Arg Tyr Ser Phe Lys Asp Thr Glu

35 40 45

Lys Ile Thr Leu Ser Glu Gly Asp Phe Val Val Leu Thr Ile Lys Glu

50 55 60

Asp Pro Lys Phe Pro Ala Arg Gly Leu Gly Tyr Ile Lys Glu Ile Asp

65 70 75 80

Trp Glu Asn Lys Lys Ala Lys Val Gln Val Glu Glu Glu Phe Arg His

85 90 95

Thr Leu Glu Lys Pro Glu Glu Arg Glu Thr Gly Ile Ile Val Arg Ser

100 105 110

Leu Asp Val Ile Glu Lys Pro Leu Glu Ile Phe Tyr Glu Gln Ile Ala

115 120 125

Lys Arg Asn Ala Thr Gly Leu Ala Ala Val Glu

130 135

<210> 217

<211> 282

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 217

Gly Asp Ala Thr Val Thr Gln Leu Arg Glu Ile Met Asp Asp Pro Ala

1 5 10 15

Gly Tyr Phe Leu Pro Asn Leu Lys His Gly Ala Asp Asn Met Phe Tyr

20 25 30

Val Gly Pro Arg Gly Leu Ala Gln Glu Leu Glu Glu Leu Phe Thr Phe

35 40 45

Pro Ser Thr Ile Leu Arg Lys Arg Gln Asp Thr Ser Gln His Asp Glu

50 55 60

Arg Gln Ala Lys Lys Ala Arg Thr Gln Glu Asp Glu Ala Ala Gly Asp

65 70 75 80

Ala Leu Glu Glu Pro Glu Thr Gly Arg Arg Asp Ser Val Leu Pro Thr

85 90 95

Glu Arg Ala Ala Phe Gly Leu Glu Gly Asp Asp Ser Gly Phe Phe Leu

100 105 110

Gly Asp Gln Thr Met Gly Asp Asp Met Leu Pro Met Asp Asp Met Gly

115 120 125

Ala Met Asp Thr Gly Val Asp Gln Arg Arg Met Arg Thr Pro Ser Val

130 135 140

Ala Pro Ser Val Thr Glu Ser Ile Ala Arg Gln Ile Gln Asn Asp Arg

145 150 155 160

Ser Ala Gly Thr His Pro Leu Ala Ile Phe Glu Lys Glu Ala Arg Asp

165 170 175

Asp Thr Gln Ser Gln Ser Gln Ala Thr Pro Asn Lys Ser Val Ala Ser

180 185 190

Glu Ser Ile Ser Lys Thr Ser Ser Gly Gln Ser Lys Asn Thr Gly Met

195 200 205

Ala Met Gly Leu Leu Arg Arg Glu Ile Glu Ala Ile Glu Glu Glu Asp

210 215 220

Lys Met Val Gly Phe Asp His Leu Ala Asp Lys Ala Ser Lys Arg Ala

225 230 235 240

Ala Ser Ala Phe Phe Phe Glu Leu Leu Val Leu Gly Thr Lys His Ala

245 250 255

Val Lys Leu Glu Gln Ala Gln Ala Phe Gly Asp Ile His Ile Arg Gly

260 265 270

Lys Asp Lys Leu Phe Ala Glu Val Val Ala

275 280

<210> 218

<211> 261

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 218

Met Ala Pro Ile Thr Cys Ser Thr Ser Val His Ala Asp Leu His Lys

1 5 10 15

Ser Ser Arg Ser Phe Leu Pro Ser Gln Ala Gln Ser Ser Glu Ser Ala

20 25 30

Arg Ile Pro Val Ser Met Thr Lys Gly Arg Gln Arg Arg Arg Ala Arg

35 40 45

Lys Arg Thr Lys Arg Leu Val Thr Arg Trp Arg Ser Pro Arg Leu Gly

50 55 60

Asp Ala Thr Val Cys Phe Arg Leu Asn Gly Pro Leu Leu Val Ser Arg

65 70 75 80

Val Met Thr Arg Ala Phe Ser Leu Ala Thr Arg Arg Trp Glu Thr Thr

85 90 95

Cys Cys Leu Trp Thr Thr Trp Glu Pro Trp Thr Pro Glu Trp Thr Ser

100 105 110

Asp Ala Cys Glu His His Gln Ser His Arg Arg Ser Pro Asn Arg Ser

115 120 125

His Val Arg Phe Arg Met Thr Glu Ala Leu Ala His Thr His Trp Leu

130 135 140

Tyr Ser Arg Arg Arg Gln Gly Thr Thr Arg Ser Arg Asn Arg Arg Leu

145 150 155 160

Arg Pro Thr Asn Arg Trp Pro Pro Ser Leu Ser Ala Arg Leu Leu Leu

165 170 175

Ala Asn Gln Arg Ile Leu Ala Trp Pro Trp Val Cys Cys Glu Gly Arg

180 185 190

Leu Arg Arg Ser Arg Arg Lys Thr Arg Trp Ser Gly Leu Ile Thr Trp

195 200 205

Gln Thr Arg Arg Pro Ser Glu Gln Arg Leu His Ser Ser Ser Ser Cys

210 215 220

Trp Cys Leu Val Pro Asn Met Arg Ser Ser Leu Asn Lys Leu Arg Leu

225 230 235 240

Ser Ala Thr Ser Thr Tyr Ala Ala Lys Thr Ser Cys Leu Gln Arg Leu

245 250 255

Leu His Arg Gln Thr

260

<210> 219

<211> 108

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 219

Met Gly Asp His Ala Thr Thr Asn Asp Pro Ser Asn Ala Thr Phe Glu

1 5 10 15

Glu Lys Gly Lys Gly Lys Asp Val Gln Asp Gln Ile Ala Glu Asp Ser

20 25 30

Ser Asp Glu Glu Ser Asp Gln Glu Pro Glu Met Val Asp Glu Glu Glu

35 40 45

Asp Asp Asn Asn Leu Glu Pro Ile Ser Gln Asp Asn Ile Ile Ser Gly

50 55 60

Gly Arg Arg Thr Arg Gly Lys Ile Ile Asp Tyr Ala Ala Glu Ala Glu

65 70 75 80

Lys Asn Lys Asp Glu Met Glu Asp Ser Glu Asp Asp Glu Asp Tyr Gln

85 90 95

Gly Ala Asn Asp Asp Glu Asp Asp Gln Met Arg Asp

100 105

<210> 220

<211> 78

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 220

Glu Leu Lys Tyr Phe Lys Ala Val Ala Leu Tyr Asn Leu Ser Arg Tyr

1 5 10 15

Leu Asp Ala Arg Lys Ala Ile Asn Asp Leu Ile Gln Ser Tyr Pro Asp

20 25 30

Phe Arg Gln Ala Glu Ala Leu Lys Ser Ala Ile Asp Asp Lys Val Val

35 40 45

Arg Asp Gly Leu Ile Gly Val Ser Val Ala Gly Ala Val Val Ala Gly

50 55 60

Val Val Gly Leu Ala Val Ala Leu Ala Arg Gly Asn Arg Gly

65 70 75

<210> 221

<211> 27

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 221

Met Gly His Val Glu Ser Arg Val Asn Gln Arg Gly Pro Pro Arg Lys

1 5 10 15

Ala Lys Tyr Ser Trp Val Thr Asp Ser Glu Lys

20 25

<210> 222

<211> 27

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 222

Met Leu Lys Ala Pro Phe Phe His Arg Pro Cys Gly Gly Lys Gly Val

1 5 10 15

Gln Leu Lys Trp Ala Thr Pro Asp Ser Ala Val

20 25

<210> 223

<211> 177

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 223

Met Pro Val Arg Pro Tyr Leu Glu Glu Met Ala Asp Met Pro Val Pro

1 5 10 15

Leu Phe Ala Tyr Asp Ala Pro Pro Thr Leu Ala Asp His Pro His Ala

20 25 30

Arg Glu His Gln His Thr Thr Phe Met Gln Tyr Leu Ala Arg Lys Gln

35 40 45

Pro Asp Pro Lys Asn Tyr Pro Asn Tyr Pro Asp Val Asp Ile Arg Asp

50 55 60

Ala Ile Asn His Tyr Leu Ile Glu Leu Glu Cys Pro Gly Ile Lys Asp

65 70 75 80

Ala Ala Asp Ile His Cys Gln Trp Thr Ser Ser Arg His Leu Thr Val

85 90 95

Thr Gly Asp Ile Ala Arg Pro Glu Glu Ser Gln Ile Glu Ala Gln Ile

100 105 110

Glu Ser Arg Pro Val Tyr Leu Val Leu Gly Glu Arg Arg Ile Gly Ser

115 120 125

Phe Arg Arg Asn Phe Thr Phe Pro Val Glu Val Glu Gln Glu Asn Met

130 135 140

Thr Ala Lys Leu Glu Ala Gly Leu Leu Lys Ile Val Leu Pro Lys His

145 150 155 160

Lys His His Thr Pro Lys Gly Thr Gly Lys Val Asp Ile Asp Val Ile

165 170 175

Glu

<210> 224

<211> 163

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 224

Met Val Leu Val Leu Gly Gln Asp Asn Leu Gln Gln Ser Gly Leu Gln

1 5 10 15

Leu Gly Ser His Ile Phe Leu Leu Asp Leu His Arg Glu Gly Lys Val

20 25 30

Ala Thr Glu Arg Ala Asn Ala Ser Leu Ser Gln Asn Gln Val Asp Gly

35 40 45

Pro Ala Leu Asp Leu Arg Phe Asp Leu Ala Phe Leu Arg Thr Gly Asp

50 55 60

Val Ala Gly Asp Gly Gln Val Pro Arg Ala Arg Pro Leu Ala Val Asp

65 70 75 80

Val Gly Cys Val Phe Asp Pro Arg Ala Phe Glu Leu Asp Gln Val Val

85 90 95

Ile Asp Gly Val Ala Asp Val His Val Arg Val Val Gly Val Val Leu

100 105 110

Trp Val Arg Leu Leu Ala Arg Lys Val Leu His Glu Gly Arg Val Leu

115 120 125

Val Leu Ala Gly Val Arg Val Val Gly Gln Gly Gly Arg Cys Val Val

130 135 140

Arg Glu Gln Gly His Gly His Val Gly His Phe Phe Gln Val Gly Ser

145 150 155 160

Tyr Gly His

<210> 225

<211> 118

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 225

Met Phe Gly Phe Asn Phe Asn Thr Thr Lys Leu Leu Lys Thr Ile Leu

1 5 10 15

Val Val Cys Tyr Leu Gln Ala Thr Val Leu Ala Asp Pro Tyr Thr Arg

20 25 30

Val Ser Trp Glu Ala Tyr Met Asn His Val Asn Gly Ser Asp Asp Tyr

35 40 45

Arg Thr Gln Gly Asp Asp Thr Arg Ala Thr Arg Phe Pro Glu Thr Lys

50 55 60

Pro Pro Lys Gln Gly Lys Asp Phe Leu Trp Ser Ser Lys Pro Val Pro

65 70 75 80

Ser Ser Asp Leu Phe Leu Glu Phe Phe Met Tyr Glu Gly Glu Pro Asp

85 90 95

Glu Phe Ser Arg Thr Thr Glu Ser Tyr Gln Ser Leu Pro Ser Asn Ala

100 105 110

Leu Thr Ala Arg Gln Lys

115

<210> 226

<211> 349

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 226

Met Ser Pro Thr Pro Thr Ser Pro His Asn Lys Leu Ser Leu Pro Ala

1 5 10 15

Arg Ala Ser Ser His Asp Ser Thr Asp Gly Ile Arg Lys Arg Val Cys

20 25 30

Lys Ala Cys Asp Arg Cys Arg Leu Lys Lys Ser Lys Cys Asp Gly Ser

35 40 45

Ser Pro Cys Ser Arg Cys Lys Ala Asp Asn Ala Ile Cys Val Phe Gly

50 55 60

Glu Arg Lys Arg Ser His Asp Lys His Tyr Pro Lys Gly Tyr Val Glu

65 70 75 80

Met Leu Glu Gln Gln Gln Gly Gln Leu Val Ser Gly Leu Lys Glu Met

85 90 95

Tyr His Arg Leu Gln Lys Ala Ser Ala Trp Asp Gly Pro Val Leu Asp

100 105 110

Glu Ser Thr Gly Gln Pro Leu Thr His Asp Ile Leu Ser Ala Leu Asp

115 120 125

Leu Leu Glu Pro Lys His Asp Asp Ser Asn Glu Pro Glu Val Phe Glu

130 135 140

Glu Asn Cys Glu Lys Leu Gln Ser Lys Leu Leu Ala Asp Gly Ala Gly

145 150 155 160

Phe Ala His Arg Arg Gly Ser Ile Ser Ser Asp Ser Glu His Ser His

165 170 175

His Asp Arg Pro Lys Thr Ser Ser Arg His Asp Thr Pro Val Gln Pro

180 185 190

Lys Pro Ser Ile Phe Lys Glu Asn Leu Ser Phe Ala Ser Ala Ala Ser

195 200 205

Ser Pro Leu Thr Gln Ser Pro Ile Pro Arg Ser Lys Pro Leu Asn Val

210 215 220

Met Pro Tyr Gln Thr Leu Gln Pro Ser Ser Arg Pro Ser Pro Leu Gln

225 230 235 240

Met Pro Ser Ala Tyr Asn Asp Pro Gln Leu Tyr Ala Pro Glu Trp Ala

245 250 255

Gln Ala Leu Ala Asp Met Ser Gly Asp Pro Asn Tyr Arg Gln Arg Phe

260 265 270

Ser Met Gln Gln Gln Gln Gln Asn Asp Phe Asp Asn Leu Leu Trp Asp

275 280 285

Pro Ser Ala Gln Ala Pro Met Glu Ser Pro Phe Ser Gln Pro Ala Phe

290 295 300

Phe Asn Gln Ala Gln Leu Ile Gly Ser Gly Asn Val Phe Gly Leu Ser

305 310 315 320

Asp Ile Asn Asp Leu Gly Pro Asn Pro Ala Asp Gly Gly Met Asp Phe

325 330 335

Asp Phe Ser Lys Phe Val Gln Gln Thr Glu Val Met Thr

340 345

<210> 227

<211> 149

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 227

Met Ser Ser Phe Arg Val Ala Ala Pro Lys Met Ala Ser Met Ala Ala

1 5 10 15

Gln Ser Ser Val Lys Val Ala Arg Pro Ala Phe Gln Ala Ala Gln Leu

20 25 30

Gln Lys Phe Thr Arg Ala Tyr Ser Ala Val Pro Lys Asn Thr Val Phe

35 40 45

Asn Thr Met Lys Arg Thr Gln Met Met Ala Arg Gln Ala Ser Pro Ile

50 55 60

Ala Lys Arg Ala Tyr Ser Ser Glu Met Ala Asn Ala Leu Val Gln Val

65 70 75 80

Ser Gln Asn Ile Gly Met Gly Ser Ala Ala Ile Gly Leu Ala Gly Ala

85 90 95

Gly Val Gly Ile Gly Leu Val Phe Ala Ala Leu Ile Gln Ala Val Ala

100 105 110

Arg Asn Pro Ser Leu Arg Gly Gln Leu Phe Ser Tyr Ala Ile Leu Gly

115 120 125

Phe Ala Phe Val Glu Ala Ile Gly Leu Phe Asp Leu Met Val Ala Met

130 135 140

Met Ala Lys Phe Leu

145

<210> 228

<211> 165

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 228

Met Glu Cys Thr Phe Leu Gln Glu Leu Gly His His Gly Asn His Glu

1 5 10 15

Val Glu Glu Thr Asp Gly Leu Asp Glu Ser Glu Thr Lys Asn Gly Val

20 25 30

Arg Glu Lys Leu Ala Thr Glu Gly Gly Val Ala Gly Asp Gly Leu Asp

35 40 45

Glu Gly Gly Glu Asp Glu Thr Asp Thr Asp Thr Ser Thr Gly Lys Thr

50 55 60

Asp Gly Gly Gly Thr His Thr Asp Val Leu Gly Asp Leu Asp Glu Gly

65 70 75 80

Val Gly His Leu Arg Gly Val Gly Thr Leu Gly Asp Gly Gly Gly Leu

85 90 95

Ala Gly His His Leu Gly Ala Leu His Gly Val Glu Asp Gly Val Leu

100 105 110

Gly Asp Arg Gly Val Gly Ala Gly Glu Leu Leu Glu Leu Ser Ser Leu

115 120 125

Glu Gly Arg Ala Gly Asp Leu His Gly Gly Leu Ser Gly His Gly Gly

130 135 140

His Leu Gly Gly Gly Asp Ala Glu Gly Arg His Cys Asp Asp Gly Glu

145 150 155 160

Val Val Arg Trp Ser

165

<210> 229

<211> 425

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 229

Gly Gly Phe Ser Val Lys Phe Arg Thr Ala Glu Gly Asn Trp Asp Phe

1 5 10 15

Val Ala Asn Asn Thr Pro Val Phe Phe Leu Arg Asp Pro Ala Lys Phe

20 25 30

Pro His Phe Ile His Thr Gln Lys Arg Asp Pro Ala Thr His Leu Ser

35 40 45

Gly Asp Asp Asp Ser Thr Met Phe Trp Asp Tyr Leu Ser Gln Asn Pro

50 55 60

Glu Ser Ile His Gln Val Met Ile Leu Met Gly Asp Arg Gly Ile Pro

65 70 75 80

Lys Gly Trp Arg Phe Met His Gly Tyr Tyr Gly His Thr Leu Lys Ile

85 90 95

Val Asn Asp Lys Gly Glu Trp Val Tyr Ala Gln Phe His Leu Ile Ser

100 105 110

Asp Gln Gly Thr Gln Asn Phe Thr Gly Asp Glu Ala Ala Gln Gln Ser

115 120 125

Asn Asp Tyr Gly Gln Lys Asp Leu Tyr Glu Ala Ile Glu Lys Gly Asp

130 135 140

Phe Pro Ser Trp Thr Met Lys Val Gln Ile Met Thr Glu Lys Gln Ala

145 150 155 160

Glu Glu Ala Trp Glu Gln Lys Arg Ile Asn Val Phe Asp Leu Thr His

165 170 175

Val Trp Pro His Gly Asp Tyr Pro Leu Arg Thr Val Gly Lys Phe Thr

180 185 190

Leu Asn Glu Asn Ala Lys Asn Tyr Phe Ala Glu Val Glu Gln Val Ala

195 200 205

Phe Asn Pro Ser His Met Ile Pro Gly Val Glu Pro Ser Asn Asp Pro

210 215 220

Val Leu Gln Ser Arg Leu Phe Ser Tyr Pro Asp Ala His Arg His Arg

225 230 235 240

Ile Gly Ala Asn Tyr Gln Gln Leu Pro Val Asn Gln Asn Val Cys Pro

245 250 255

Phe Ala Leu Gly Asn Phe Gln Arg Asp Gly Gln Met Ala Phe Tyr Asn

260 265 270

Gln Gly Ser Arg Pro Asn Tyr Leu Ser Ser Ile Glu Pro Ile Ser Phe

275 280 285

Lys Glu Arg Ala Tyr Asp Leu Asn Lys Val His Gly Lys Phe Val Gly

290 295 300

Glu Ala Val Ala Phe Leu Ser Glu Ile Arg Pro Glu Asp Phe Asn Ala

305 310 315 320

Pro Arg Ala Leu Trp Gln Lys Val Phe Ser Glu Glu Ser Lys Gln Arg

325 330 335

Phe Val Asp Thr Val Ser Gly His Met Ser Thr Val Arg Asp Lys Ala

340 345 350

Ile Thr Ala Arg Met Met Thr Ile Phe Arg Glu Val Ser Pro Asp Leu

355 360 365

Gly Asp Arg Leu Glu Lys Ala Thr Gly Val Lys Gly Glu Ser Thr Ile

370 375 380

Ala Gly Met Lys Phe Asn Gly Thr His Asn Gly Phe Asp Lys Ala Asn

385 390 395 400

Lys Ile Pro Ala Asn Gly Met Lys Lys Gly Gly Glu Val Ile Phe Asp

405 410 415

Asn Gly Ala Pro Ala Thr Ala Ala Arg

420 425

<210> 230

<211> 239

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 230

Val Ser Val Ser Ile Gly Val Arg Glu Gln Ser Arg Leu Gln His Trp

1 5 10 15

Val Val Gly Arg Leu Asp Thr Gly Asn His Val Arg Arg Val Glu Cys

20 25 30

Asp Leu Phe His Leu Gly Glu Val Val Leu Gly Ile Leu Val Lys Gly

35 40 45

Glu Phe Thr Asp Cys Ser Lys Trp Val Ile Thr Met Arg Pro Asp Val

50 55 60

Gly Gln Ile Lys Asp Val Asp Pro Leu Leu Leu Pro Cys Leu Leu Gly

65 70 75 80

Leu Leu Leu Gly His Asp Leu Asn Leu His Arg Pro Arg Gly Glu Val

85 90 95

Ser Leu Leu Asp Gly Phe Val Gln Ile Leu Leu Ser Val Ile Val Gly

100 105 110

Leu Leu Ser Ser Leu Val Thr Arg Glu Val Leu Gly Ala Leu Ile Arg

115 120 125

Asp Glu Val Glu Leu Gly Val Asp Pro Phe Ala Leu Val Ile Asp Asn

130 135 140

Leu Glu Gly Val Ala Val Val Ala Met His Glu Ser Pro Ala Leu Gly

145 150 155 160

Asp Thr Ser Ile Thr His Glu Asp His Asp Leu Val Asp Arg Leu Gly

165 170 175

Val Leu Arg Gln Val Val Pro Glu His Gly Arg Val Ile Val Thr Arg

180 185 190

Gln Val Gly Gly Gly Ile Ser Leu Leu Gly Val Asp Glu Val Gly Glu

195 200 205

Leu Gly Arg Val Ser Glu Glu Glu Asp Gly Gly Val Val Gly His Lys

210 215 220

Val Pro Ile Ser Phe Cys Gly Pro Glu Leu Asp Arg Glu Ser Ser

225 230 235

<210> 231

<211> 368

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 231

Asp Thr Ile Asp Ala Glu Val Leu Asp Ser Leu Gly Val Thr Gln Glu

1 5 10 15

Asn Phe Gln Phe Ala Leu Gly Val Ser Asn Pro Ser Ala Leu Arg Glu

20 25 30

Val Ala Val Val Glu Val Pro Asn Val Arg Trp Glu Asp Ile Gly Gly

35 40 45

Leu Glu Glu Val Lys Arg Glu Leu Ile Glu Ser Val Gln Tyr Pro Val

50 55 60

Asp His Pro Glu Lys Phe Leu Lys Phe Gly Met Ser Pro Ser Lys Gly

65 70 75 80

Val Leu Phe Tyr Gly Pro Pro Gly Thr Gly Lys Thr Leu Leu Ala Lys

85 90 95

Ala Val Ala Asn Glu Cys Ala Ala Asn Phe Ile Ser Val Lys Gly Pro

100 105 110

Glu Leu Leu Ser Met Trp Phe Gly Glu Ser Glu Ser Asn Ile Arg Asp

115 120 125

Ile Phe Asp Lys Ala Arg Ala Ala Ala Pro Cys Val Val Phe Leu Asp

130 135 140

Glu Leu Asp Ser Ile Ala Lys Ser Arg Gly Gly Ser Gln Gly Asp Ala

145 150 155 160

Gly Gly Ala Ser Asp Arg Val Val Asn Gln Leu Leu Thr Glu Met Asp

165 170 175

Gly Met Thr Ser Lys Lys Asn Val Phe Val Ile Gly Ala Thr Asn Arg

180 185 190

Pro Glu Gln Leu Asp Asn Ala Leu Cys Arg Pro Gly Arg Leu Asp Thr

195 200 205

Leu Val Tyr Val Pro Leu Pro Asp Gln Glu Gly Arg Glu Ser Ile Leu

210 215 220

Lys Ala Gln Leu Arg Lys Thr Pro Ile Ala Asp Asp Ile Asp Leu Ser

225 230 235 240

Tyr Met Ala Ser Lys Thr His Gly Phe Ser Gly Ala Asp Leu Gly Phe

245 250 255

Ile Thr Gln Arg Ala Val Lys Leu Ala Ile Lys Gln Ser Ile Asp Leu

260 265 270

Ala Ile Gln Asn Gln Lys Ala Arg Glu Ala Glu Gly Asp Thr Ala Met

275 280 285

Asp Glu Asp Ile Glu Glu Asp Asp Pro Val Pro Glu Leu Thr Lys Ala

290 295 300

His Phe Glu Glu Ala Met Ser Met Ala Arg Arg Ser Val Thr Asp Thr

305 310 315 320

Glu Ile Arg Arg Tyr Glu Ala Phe Ala Gln Ser Met Lys Ser Ser Gly

325 330 335

Gly Gly Ser Ala Phe Phe Arg Phe Pro Glu Ser Gly Ala Asp Gly Asn

340 345 350

Ala Ala Glu Gln Gln Gln Asn Gly Ala Gly Glu Glu Asp Leu Tyr Asp

355 360 365

<210> 232

<211> 317

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 232

Met His Asp Cys Ser Leu His Leu Glu Tyr Tyr Ser Asp Asn Ser Lys

1 5 10 15

Gly Leu Gly Arg Glu Val Arg Glu Thr Asn Leu Val Val Glu Val Leu

20 25 30

Leu Ala Ser Thr Ile Leu Leu Leu Leu Gly Cys Val Ala Ile Gly Thr

35 40 45

Ala Leu Arg Glu Ala Glu Glu Ser Ala Ala Ala Thr Gly Ala Leu His

50 55 60

Ala Leu Gly Glu Ser Leu Val Ala Pro Asp Leu Gly Val Gly Asp Gly

65 70 75 80

Ala Thr Ser His Ala His Ser Leu Leu Lys Val Ser Leu Gly Gln Leu

85 90 95

Gly His Gly Val Val Leu Leu Asp Val Leu Val His Gly Gly Val Thr

100 105 110

Leu Gly Leu Ser Ser Leu Leu Val Leu Asp Gly Gln Val Asn Arg Leu

115 120 125

Leu Asp Gly Gln Leu Asp Gly Thr Leu Gly Asp Glu Ala Lys Ile Gly

130 135 140

Thr Arg Glu Thr Val Ser Leu Gly Gly His Val Gly Lys Val Asp Val

145 150 155 160

Val Gly Asp Arg Ser Leu Ala Glu Leu Gly Leu Glu Asn Ala Leu Thr

165 170 175

Ala Leu Leu Val Arg Gln Gly Asn Val Asp Glu Ser Val Glu Thr Thr

180 185 190

Arg Thr Ala Glu Ser Val Val Glu Leu Leu Arg Pro Val Gly Gly Thr

195 200 205

Asp Asp Glu Asn Val Leu Leu Ala Gly His Thr Val His Leu Ser Glu

210 215 220

Lys Leu Val Asp His Thr Val Gly Ser Thr Ala Ser Ile Ala Leu Arg

225 230 235 240

Thr Ala Thr Arg Leu Gly Asp Gly Val Gln Leu Val Glu Glu Asp Asn

245 250 255

Ala Arg Arg Gly Ser Thr Ser Leu Val Glu Asp Val Thr Asn Val Ala

260 265 270

Leu Arg Leu Thr Glu Pro His Gly Glu Lys Leu Gly Thr Leu Asp Gly

275 280 285

Asn Lys Val Gly Arg Ala Leu Val Gly Asp Ser Leu Gly Gln Lys Ser

290 295 300

Leu Thr Ser Thr Arg Gly Thr Val Glu Lys His Thr Leu

305 310 315

<210> 233

<211> 166

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 233

Val Ser Pro Lys Arg Thr Ser Ser Leu Pro Leu Ala Ser Ala Thr Pro

1 5 10 15

Leu Pro Phe Ala Arg Ser Gln Trp Ser Arg Phe Pro Thr Ser Asp Gly

20 25 30

Arg Thr Leu Val Val Ser Arg Arg Ser Arg Gly Ser Ser Ser Arg Ala

35 40 45

Cys Asn Thr Pro Ser Thr Thr Pro Arg Ser Ser Ser Ser Leu Ala Cys

50 55 60

Pro His Gln Arg Val Cys Phe Ser Thr Val Pro Leu Val Leu Val Arg

65 70 75 80

Leu Phe Trp Pro Arg Leu Ser Pro Thr Ser Ala Arg Pro Thr Leu Phe

85 90 95

Pro Ser Arg Val Pro Ser Phe Ser Pro Cys Gly Ser Val Ser Leu Arg

100 105 110

Ala Thr Phe Val Thr Ser Ser Thr Arg Leu Val Leu Pro Arg Leu Ala

115 120 125

Leu Ser Ser Ser Thr Ser Trp Thr Pro Ser Pro Ser Leu Val Ala Val

130 135 140

Leu Arg Ala Met Leu Ala Val Leu Pro Thr Val Trp Ser Thr Ser Phe

145 150 155 160

Ser Leu Arg Trp Thr Val

165

<210> 234

<211> 160

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 234

Met Gly His Ser Ala Gly Leu Arg Lys Gly Thr Arg Tyr Ala Phe Ser

1 5 10 15

Arg Asp Phe Lys Lys Arg Gly Met Ile Pro Leu Ser Thr Tyr Leu Lys

20 25 30

Gln Tyr Lys Val Gly Asp Ile Val His Val Val Cys Asn Gly Ala Val

35 40 45

Gln Lys Gly Met Pro His Lys Asp Phe His Gly Lys Thr Gly Val Val

50 55 60

Tyr Asn Val Thr Lys Ser Ala Val Gly Val Ile Leu Tyr Lys Gln Val

65 70 75 80

Gly Asn Arg Tyr Ile Glu Lys Arg Val Asn Leu Arg Ile Glu His Val

85 90 95

Arg Leu Ser Arg Ser Arg Glu Glu Phe Ile Val Arg Val Lys Thr Asn

100 105 110

Ala Glu Lys Lys Arg Lys Ala Lys Glu Glu Gly Thr Thr Val Phe Leu

115 120 125

Lys Arg Gln Ala Asp Lys Pro Arg Glu Ala Arg Thr Ile Ser Ala Lys

130 135 140

Asp Asn Lys Pro Glu Ser Ile Ala Pro Ile Ala Tyr Asp Thr His Ile

145 150 155 160

<210> 235

<211> 526

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 235

Asp Met Gly Ile Gly Gly Leu Asp Thr Glu Phe Ser Ala Ile Phe Arg

1 5 10 15

Arg Ala Phe Ala Ser Arg Ile Phe Pro Pro Gly Leu Val Glu Lys Leu

20 25 30

Gly Ile Gln His Val Lys Gly Ile Leu Leu Phe Gly Pro Pro Gly Thr

35 40 45

Gly Lys Thr Leu Met Ala Arg Gln Ile Gly Thr Met Leu Asn Ala Arg

50 55 60

Glu Pro Lys Val Val Asn Gly Pro Glu Ile Leu Asn Lys Phe Val Gly

65 70 75 80

Gln Ser Glu Glu Asn Ile Arg Lys Leu Phe Ala Asp Ala Glu Lys Glu

85 90 95

Gln Lys Glu Lys Gly Asp Glu Ser Gly Leu His Ile Ile Ile Phe Asp

100 105 110

Glu Leu Asp Ala Ile Cys Lys Gln Arg Gly Ser Thr Asn Ser Gly Thr

115 120 125

Gly Val Gly Asp Ser Val Val Asn Gln Leu Leu Ser Lys Met Asp Gly

130 135 140

Val Asp Gln Leu Asn Asn Val Leu Ile Ile Gly Met Thr Asn Arg Met

145 150 155 160

Asp Met Ile Asp Glu Ala Leu Leu Arg Pro Gly Arg Leu Glu Val His

165 170 175

Ile Glu Ile Ser Leu Pro Asp Glu Ala Gly Arg Phe Gln Ile Leu Asn

180 185 190

Ile His Thr Asn Lys Met Arg Thr Asn Gly Val Met Asp Ser Asp Val

195 200 205

Asp Leu Gly Glu Leu Ala Ala Leu Thr Lys Asn Phe Ser Gly Ala Glu

210 215 220

Ile Gly Gly Leu Val Lys Ser Ala Thr Ser Phe Ala Phe Asn Arg His

225 230 235 240

Val Lys Val Gly Ser Val Ala Ala Phe Asp Asp Ile Asp Asn Met Lys

245 250 255

Ile Ser Arg Ala Asp Phe Leu His Ala Leu Asp Glu Val Thr Pro Ala

260 265 270

Phe Gly Val Ser Glu Glu Glu Leu Gln Gln Val Val Gln Asn Gly Ile

275 280 285

Ile His Tyr Ser Gln His Val Asn Asp Thr Leu Asn Asp Gly Ser Leu

290 295 300

Leu Val Glu Gln Val Arg Lys Ser Asp Arg Thr Pro Leu Val Ser Ala

305 310 315 320

Leu Leu His Gly Pro Ser Gly Ala Gly Lys Thr Ala Leu Ala Ala Thr

325 330 335

Ile Ala Met Ala Ser Glu Phe Pro Phe Ile Lys Leu Ile Ser Pro Glu

340 345 350

Thr Met Val Gly Phe Ser Glu Pro Gln Lys Ile Ala Gln Leu Asn Lys

355 360 365

Val Phe Thr Asp Ser Tyr Lys Ser Pro Met Ser Ile Ile Val Val Asp

370 375 380

Ser Leu Glu Arg Leu Leu Asp Trp Asn Pro Ile Gly Pro Arg Phe Ser

385 390 395 400

Asn Gly Val Leu Gln Ala Leu Val Val Leu Phe Gly Lys Arg Pro Pro

405 410 415

Lys Gly Arg Arg Leu Leu Ile Leu Ala Thr Thr Ser Asn Arg Asn Ile

420 425 430

Leu Thr Asp Met Asp Val Leu Ser Ala Phe Asp Thr Asp Ile Pro Ile

435 440 445

Asn Pro Ile Ser Ser Ile Asp Ala Val Val His Val Leu Asp Glu Val

450 455 460

Lys Leu Phe Pro Asn Ser Lys Glu Lys Gln Arg Ala Thr Gln Met Leu

465 470 475 480

Arg Glu Ala Arg Leu Gly Glu Gly Gly Arg Pro Asp Leu Leu Val Gly

485 490 495

Val Lys Lys Leu Leu Ser Met Ala Glu Met Ala Arg Gln Asp Pro Asp

500 505 510

Pro Thr Met Lys Ile Val Thr Ser Ile Leu Arg Glu Ala Ser

515 520 525

<210> 236

<211> 425

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 236

Gly Gly Phe Ser Val Lys Phe Arg Thr Ala Glu Gly Asn Trp Asp Phe

1 5 10 15

Val Ala Asn Asn Thr Pro Val Phe Phe Leu Arg Asp Pro Ala Lys Phe

20 25 30

Pro His Phe Ile His Thr Gln Lys Arg Asp Pro Ala Thr His Leu Ser

35 40 45

Gly Asp Asp Asp Ser Thr Met Phe Trp Asp Tyr Leu Ser Gln Asn Pro

50 55 60

Glu Ser Ile His Gln Val Met Ile Leu Met Gly Asp Arg Gly Ile Pro

65 70 75 80

Lys Gly Trp Arg Phe Met His Gly Tyr Tyr Gly His Thr Leu Lys Ile

85 90 95

Val Asn Asp Lys Gly Glu Trp Val Tyr Ala Gln Phe His Leu Ile Ser

100 105 110

Asp Gln Gly Thr Gln Asn Phe Thr Gly Asp Glu Ala Ala Gln Gln Ser

115 120 125

Asn Asp Tyr Gly Gln Lys Asp Leu Tyr Glu Ala Ile Glu Lys Gly Asp

130 135 140

Phe Pro Ser Trp Thr Met Lys Val Gln Ile Met Thr Glu Lys Gln Ala

145 150 155 160

Glu Glu Ala Trp Glu Gln Lys Arg Ile Asn Val Phe Asp Leu Thr His

165 170 175

Val Trp Pro His Gly Asp Tyr Pro Leu Arg Thr Val Gly Lys Phe Thr

180 185 190

Leu Asn Glu Asn Ala Lys Asn Tyr Phe Ala Glu Val Glu Gln Val Ala

195 200 205

Phe Asn Pro Ser His Met Ile Pro Gly Val Glu Pro Ser Asn Asp Pro

210 215 220

Val Leu Gln Ser Arg Leu Phe Ser Tyr Pro Asp Ala His Arg His Arg

225 230 235 240

Ile Gly Ala Asn Tyr Gln Gln Leu Pro Val Asn Gln Asn Val Cys Pro

245 250 255

Phe Ala Leu Gly Asn Phe Gln Arg Asp Gly Gln Met Ala Phe Tyr Asn

260 265 270

Gln Gly Ser Arg Pro Asn Tyr Leu Ser Ser Ile Glu Pro Ile Ser Phe

275 280 285

Lys Glu Arg Ala Tyr Asp Leu Asn Lys Val His Gly Lys Phe Val Gly

290 295 300

Glu Ala Val Ala Phe Leu Ser Glu Ile Arg Pro Glu Asp Phe Asn Ala

305 310 315 320

Pro Arg Ala Leu Trp Gln Lys Val Phe Ser Glu Glu Ser Lys Gln Arg

325 330 335

Phe Val Asp Thr Val Ser Gly His Met Ser Thr Val Arg Asp Lys Ala

340 345 350

Ile Thr Ala Arg Met Met Thr Ile Phe Arg Glu Val Ser Pro Asp Leu

355 360 365

Gly Asp Arg Leu Glu Lys Ala Thr Gly Val Lys Gly Glu Ser Thr Ile

370 375 380

Ala Gly Met Lys Phe Asn Gly Thr His Asn Gly Phe Asp Lys Ala Asn

385 390 395 400

Lys Ile Pro Ala Asn Gly Met Lys Lys Gly Gly Glu Val Ile Phe Asp

405 410 415

Asn Gly Ala Pro Ala Thr Ala Ala Arg

420 425

<210> 237

<211> 122

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 237

Met Leu Thr Asp Thr Glu Ser Glu Pro Thr Ile Ser Asn Cys Pro Leu

1 5 10 15

Thr Arg Met Cys Ala Pro Ser Pro Trp Ala Thr Ser Ser Glu Thr Ala

20 25 30

Arg Trp His Ser Thr Ile Lys Val Val Asp Pro Thr Thr Phe Leu Arg

35 40 45

Leu Ser Gln Ser His Ser Arg Arg Gly Arg Met Ile Ser Thr Arg Ser

50 55 60

Thr Ala Asn Ser Ser Glu Lys Pro Ser Pro Ser Cys Leu Lys Ser Gly

65 70 75 80

Gln Arg Thr Ser Met Pro Gln Gly His Cys Gly Arg Lys Ser Leu Ala

85 90 95

Arg Lys Ala Ser Ser Asp Ser Ser Thr Pro Ser Leu Val Thr Cys Arg

100 105 110

Gln Ser Glu Thr Lys Pro Ser Pro Leu Glu

115 120

<210> 238

<211> 309

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 238

Met Pro Pro Arg Gln Pro Ala Thr Arg Leu Phe Ala Leu Pro Pro Arg

1 5 10 15

Phe Leu Cys Pro Ser Leu Pro Thr Thr Gln Thr Arg Thr Ile Arg Ser

20 25 30

Ile Asp Lys Pro Ala Pro Lys Pro Ser Arg Phe Asn Ala Ser Leu Asn

35 40 45

Leu Pro Val Leu Gly Ser Ser Ser Thr Ala Ala Phe Ala Arg Lys Glu

50 55 60

His Ser Leu Pro Leu Arg Thr Gly Ala Leu Ala Ile Lys Lys Gly Met

65 70 75 80

Thr Ala Leu Phe Asp Pro Val Thr Ala Lys Arg Thr Pro Cys Thr Val

85 90 95

Leu Gln Leu Asp Arg Cys Gln Val Val Ser His Lys Arg Arg Asp Ile

100 105 110

His Gly Tyr Trp Ala Val Gln Val Gly Ala Gly Ala Lys Glu Ala Arg

115 120 125

Asn Val Thr Arg Pro Glu Arg Gly His Phe Ala Ala Tyr Asn Val Pro

130 135 140

Leu Ser Arg His Leu Ala Glu Phe Arg Val Lys Asn Ala Glu Gly Leu

145 150 155 160

Pro Pro Val Gly Ser Ala Ile Thr Ala Asp Leu Phe Ile Glu Gly Gln

165 170 175

Phe Ile Asp Ala Lys Ala Asp Arg Arg Gly Met Gly Phe Glu Gly Gly

180 185 190

Met Lys Arg Trp Asn Phe Gly Gly Gln Pro Ala Ser His Gly Asn Ser

195 200 205

Leu Ala His Arg Leu Met Gly Ser Ser Gly Gly Gly Gln Gly Ser Gly

210 215 220

Ser Arg Val Leu Pro Gly Lys Lys Met Pro Gly Arg Met Gly Gly Glu

225 230 235 240

Gln Ala Thr Val Ala Asn Leu Arg Val Met Gln Val Asp Lys Glu Asn

245 250 255

Gly Ile Val Val Val Ser Gly Ala Val Pro Gly Pro Lys Asn Cys Met

260 265 270

Val Lys Leu Gln Asp Ala Leu Lys Lys Pro Trp Pro Asp Ala Thr Trp

275 280 285

Pro Pro Ser Ile Glu Gly Ala Thr Glu Val Leu Arg Glu Ala Thr Glu

290 295 300

Lys Ala Pro Ala Ala

305

<210> 239

<211> 130

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 239

Met Gly Ile Trp Asp Ala Phe Thr Asp Ile Val Glu Ala Val Thr Pro

1 5 10 15

Trp Ser Val Val Glu Ala Glu Ala Pro Ala Glu Glu Pro Gln Glu Glu

20 25 30

Asn Glu Ser Lys Thr Glu Ser Lys Asp Glu Pro Glu Glu Glu Glu Glu

35 40 45

Asp Glu Glu Glu Glu Glu Asp Glu Asp Asp Glu Glu Glu Leu Val Asp

50 55 60

Pro Lys Glu Thr Leu Glu Glu Glu Cys Lys Asn Ser Pro Gln Cys Ala

65 70 75 80

Pro Ala Lys His His Phe Asp Glu Cys Val Glu Arg Val Gln Gln Gln

85 90 95

Glu Ser Glu Gly Gly Ala Lys Glu Asp Cys Val Glu Glu Phe Phe His

100 105 110

Leu Ala His Cys Ala Thr Ala Cys Ala Ala Pro Lys Leu Trp Ser Gln

115 120 125

Leu Lys

130

<210> 240

<211> 136

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 240

Met Arg Lys Lys Arg Arg Met Arg Met Met Arg Arg Ser Ser Ser Thr

1 5 10 15

Pro Arg Arg Leu Ser Arg Lys Ser Ala Arg Thr Leu Leu Asn Val Pro

20 25 30

Pro Pro Ser Thr Thr Ser Thr Ser Val Leu Ser Ala Phe Ser Ser Arg

35 40 45

Arg Ala Arg Val Val Leu Arg Arg Thr Val Ser Arg Ser Ser Ser Thr

50 55 60

Leu Pro Thr Val Arg Pro Leu Ala Pro Leu Pro Ser Phe Gly Leu Ser

65 70 75 80

Ser Ser Lys Leu Thr Thr Leu Gly Tyr Arg Leu Leu Arg Arg Arg Asn

85 90 95

Gly Tyr Ile His Val Glu Lys Met Pro Gly Ala Gly Thr Arg Gln Cys

100 105 110

Cys Pro Leu Arg Lys Ala Val Pro Leu Tyr Glu Cys Ser Ser Ala Gly

115 120 125

Tyr Gln Val Gly Gln Arg Leu Leu

130 135

<210> 241

<211> 194

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 241

Gly Arg Tyr Tyr Arg Ala Pro Glu Ile Met Leu Thr Trp Gln Lys Tyr

1 5 10 15

Asp Val Ala Val Asp Ile Trp Ser Thr Gly Cys Ile Phe Ala Glu Met

20 25 30

Leu Glu Gly Lys Pro Leu Phe Pro Gly Lys Asp His Val Asn Gln Phe

35 40 45

Ser Ile Ile Thr Glu Leu Leu Gly Thr Pro Pro Asp Asp Val Ile Gln

50 55 60

Thr Ile Ala Ser Glu Asn Thr Leu Arg Phe Val Gln Ser Leu Pro Lys

65 70 75 80

Arg Glu Lys Val Pro Phe Thr Thr Lys Phe Ala Asn Ala Asp Pro Leu

85 90 95

Ser Leu Asp Leu Leu Glu Lys Met Leu Val Phe Asp Pro Arg Thr Arg

100 105 110

Ile Ser Ala Ser Glu Gly Leu Ser His Glu Tyr Leu Ala Pro Tyr His

115 120 125

Asp Pro Thr Asp Glu Pro Val Ala Ala Glu Val Phe Asp Trp Ser Phe

130 135 140

Asn Asp Ala Asp Leu Pro Val Asp Thr Trp Lys Val Met Met Tyr Ser

145 150 155 160

Glu Ile Leu Asp Phe His Asn Leu Gly Asp Ile Gln Gln Asp Gln Ala

165 170 175

Ala Glu Gly Pro Val Thr Gly Asp Leu Ala Pro Pro Ser Ala Thr Thr

180 185 190

Ser Ala

<210> 242

<211> 134

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 242

Met Ser Pro Ser Thr Phe Gly Ala Gln Asp Val Ser Ser Pro Arg Cys

1 5 10 15

Ser Arg Glu Ser Pro Cys Ser Arg Ala Arg Thr Thr Leu Ile Ser Ser

20 25 30

Arg Ser Ser Gln Asn Cys Ser Ala His Leu Leu Thr Met Ser Ser Arg

35 40 45

Pro Ser His Leu Arg Thr Pro Ser Asp Ser Ser Ser Arg Cys Pro Ser

50 55 60

Val Arg Arg Ser His Ser Leu Arg Asn Ser Pro Met Pro Thr Arg Phe

65 70 75 80

Arg Leu Thr Cys Trp Arg Arg Cys Leu Ser Ser Ile His Val Pro Val

85 90 95

Ser Arg His Gln Lys Gly Cys Arg Thr Ser Thr Leu Arg His Thr Met

100 105 110

Thr Arg Arg Met Ser Pro Ser Leu Pro Arg Cys Leu Thr Gly Val Ser

115 120 125

Thr Met Arg Ile Tyr Gln

130

<210> 243

<211> 111

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 243

Gly Tyr Pro Leu Asn Leu Ser Ile Ser Ile Ser Gly Gly Lys Glu Thr

1 5 10 15

Asn Arg Asp Cys Pro Ser Asn Gly Glu Arg Ser Gly Lys Ser Ser Asn

20 25 30

Leu Lys Ala Gly Pro Leu Gly Val Arg Ile Val Ile Cys Arg Gly Cys

35 40 45

Phe Gly Asn Gly Pro His Leu Ser Ala Leu Glu Arg Ala Val Ile Glu

50 55 60

Gly Glu Asn Pro Val Trp Asp Gly Val Ala Ala Pro Val Ser Ser Ser

65 70 75 80

Phe Asp Glu Ser Ser Cys Leu Gly Met Gln Leu Lys Leu Gly Gly Lys

85 90 95

Phe His Leu Lys Leu Asn Ile Gly Arg Arg Pro Ile Ala His Lys

100 105 110

<210> 244

<211> 41

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 244

Gly Leu Val Ala Ile Ala Arg Arg Pro His Pro Phe Pro Cys Gln Thr

1 5 10 15

Arg Lys Leu Ser Val Ser Ala Pro Lys Val Val Gly Gly Ser Pro Pro

20 25 30

Val Arg Ile Gly Arg Cys Gln Ala Lys

35 40

<210> 245

<211> 218

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 245

Met Glu Pro Asp Gln Glu Glu Ser Glu Glu Glu Glu Glu Glu Glu Asp

1 5 10 15

Asp Glu Met Asp Glu Asp Glu Asp Glu Gly Gln Gln Gln Asp Ala Ser

20 25 30

Gly Met Gln Thr Pro Ser Gly Leu Ala Thr Pro Ser Gly Tyr Ala Ser

35 40 45

Thr Thr Ser Thr Met Pro Gly Gly Met Glu Thr Pro Asp Phe Met Asp

50 55 60

Leu Arg Lys Gln Arg Gln Thr Arg Asp Glu Thr Ala Asp Gln Glu Asp

65 70 75 80

Gln Gly Ala Pro Arg Asp Leu Tyr Thr Val Val Pro Glu Arg Arg Ala

85 90 95

Thr Ala Ser Gly Phe Leu Gly Ser Asp Arg Ala Tyr Asp Leu Ser Asn

100 105 110

Ala Pro Gln Ser Ser Asn Met Pro Val Leu Gly Gln Glu Asp Ser Arg

115 120 125

Lys Lys Lys Gly Gly Arg Ser Gly Ala Asp Asp Val Asp Leu Ala Leu

130 135 140

Asp Pro Ala Glu Leu Glu Gly Met Ser Glu Gln Glu Leu Arg Gln Lys

145 150 155 160

Tyr Asp Ser His Arg Arg Ser Ser Ser Ser Gln Gly Ala Gly Gly Gln

165 170 175

Gln Asp Lys Glu Asp Phe Ser Asp Phe Val Ala Gln Glu Val Ala Lys

180 185 190

Lys Arg Gln Arg Ala Gln Gln Arg Gly Gly Ser Gly Arg Asp Arg Glu

195 200 205

Ser Ser Arg Ser Lys Glu Lys Phe Lys Phe

210 215

<210> 246

<211> 210

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 246

Met Thr Arg Trp Met Lys Met Arg Met Arg Ala Ser Ser Arg Thr Pro

1 5 10 15

Val Ala Cys Arg His Pro Leu Gly Ser Pro Arg Pro Gln Ala Met Pro

20 25 30

Leu Leu His Leu Gln Cys Leu Val Ala Trp Arg Arg Leu Thr Leu Trp

35 40 45

Thr Cys Ala Ser Ser Asp Arg Arg Ala Thr Arg Pro Leu Ile Lys Arg

50 55 60

Thr Arg Val His Arg Glu Thr Ser Ile Arg Ser Cys Pro Ser Ala Glu

65 70 75 80

Pro Pro Leu Leu Ala Ser Ser Val Leu Thr Ala Pro Met Thr Cys Pro

85 90 95

Met Arg His Ser Leu Pro Thr Cys Leu Cys Trp Val Lys Lys Thr Arg

100 105 110

Ala Arg Arg Lys Ala Ala Asp Leu Val Gln Thr Thr Ser Thr Trp Pro

115 120 125

Trp Ile Gln Leu Ser Ser Arg Ala Cys Leu Ser Lys Ser Leu Gly Arg

130 135 140

Ser Thr Thr Arg Thr Gly Ala Pro Arg Pro Val Lys Ala Pro Ala Asp

145 150 155 160

Ser Arg Thr Lys Lys Ile Ser Gln Ile Ser Ser Arg Lys Arg Ser Gln

165 170 175

Arg Arg Gly Arg Gly Leu Ser Ser Ala Ala Ala Val Asp Ala Thr Ala

180 185 190

Lys Ala Leu Gly Ala Arg Lys Ser Ser Ser Phe Arg Val Tyr Val Cys

195 200 205

Ile Val

210

<210> 247

<211> 164

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 247

Gly Val Ile Gln Val Asp Glu Pro Ala Leu Arg Glu Gly Leu Pro Leu

1 5 10 15

Arg Thr Gly Ala Ala Arg Asp Ala Tyr Val Lys Trp Ala Val Asn Ala

20 25 30

Phe Lys Leu Ser Thr Ala Gly Val Thr Asp Ser Thr Gln Val His Ser

35 40 45

His Phe Cys Tyr Ser Glu Phe Gln Asp Phe Phe His Ala Ile Ala Ala

50 55 60

Leu Asp Thr Asp Val Leu Ser Ile Glu Asn Ser Lys Ser Asp Ala Lys

65 70 75 80

Leu Leu Gln Val Phe Val Asp Gln Ser Phe Pro Ala His Ile Gly Pro

85 90 95

Gly Val Tyr Asp Ile His Ser Pro Arg Val Pro Ser Val Asp Glu Ile

100 105 110

Lys Glu Arg Ile Glu Gln Met Leu Gln Tyr Leu Lys Pro Glu Gln Leu

115 120 125

Trp Ile Asn Pro Asp Cys Gly Leu Lys Thr Arg Thr Thr Glu Gln Thr

130 135 140

Ile Gly Gln Leu Thr Ser Leu Val Glu Ala Ala Lys Phe Tyr Arg Gln

145 150 155 160

Lys Tyr Thr Gln

<210> 248

<211> 125

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 248

Val Arg Val Phe Leu Cys Val Leu Ala Leu Pro Val Met Leu Met Leu

1 5 10 15

Ser Gly Leu Ser Met Leu Ser Ser Cys Leu Leu Leu Val Ser Pro Thr

20 25 30

Ala Pro Arg Ser Thr Pro Thr Ser Ala Thr Val Asn Ser Arg Thr Ser

35 40 45

Ser Thr Leu Leu Leu Pro Leu Ile Pro Met Phe Cys Pro Ser Arg Thr

50 55 60

Ala Ser Pro Met Pro Ser Ser Ser Arg Ser Ser Leu Ile Arg Val Ser

65 70 75 80

Pro Pro Thr Leu Asp Leu Val Ser Thr Ile Ser Thr Leu Leu Val Phe

85 90 95

Pro Pro Trp Met Arg Ser Arg Ser Val Ser Ser Arg Cys Ser Ser Thr

100 105 110

Ser Ser Leu Ser Ser Ser Gly Ser Thr Leu Thr Ala Val

115 120 125

<210> 249

<211> 171

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 249

Met Leu Met Arg Thr Glu Leu Leu Ser Val Leu Leu Ala Val Glu Leu

1 5 10 15

Ser Ser Leu Asp Gln Arg Gly Gln Leu Ala Asp Ser Leu Leu Ser Gly

20 25 30

Thr Gly Leu Gln Thr Ala Val Arg Val Asp Pro Glu Leu Leu Arg Leu

35 40 45

Glu Val Leu Glu His Leu Leu Asp Thr Leu Leu Asp Leu Ile His Gly

50 55 60

Gly Asn Thr Arg Arg Val Asp Ile Val Asp Thr Arg Ser Asn Val Gly

65 70 75 80

Gly Glu Thr Leu Ile Asn Glu Asp Leu Glu Glu Leu Gly Ile Gly Leu

85 90 95

Ala Val Leu Asp Gly Gln Asn Ile Gly Ile Lys Gly Ser Asn Ser Val

100 105 110

Glu Glu Val Leu Glu Phe Thr Val Ala Glu Val Gly Val Asp Leu Gly

115 120 125

Ala Val Gly Asp Thr Ser Ser Arg Gln Leu Glu Ser Ile Asp Ser Pro

130 135 140

Leu Asn Ile Ser Ile Thr Gly Ser Ala Ser Thr Gln Arg Lys Thr Leu

145 150 155 160

Thr Gln Gly Arg Leu Val Asp Leu Asp Asp Pro

165 170

<210> 250

<211> 66

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 250

Leu Lys Ser Gly Val Phe Gly Val Arg Val Val Ile Cys Arg Gly Cys

1 5 10 15

Phe Trp Ala Ala Thr Asp Leu Ser Ser Leu Glu Gln Asp Val Ile Glu

20 25 30

Gly Glu Asn Pro Val Cys Gly Arg Lys Gly Thr Leu His Val Ala Pro

35 40 45

Ser Thr Ser Arg Val Val Trp Glu Cys Ser Ser Lys Trp Glu Val Asn

50 55 60

Phe Phe

65

<210> 251

<211> 189

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 251

Met Lys Leu Ser Asn Ser Ala His Tyr Ser Leu Phe Leu Leu Ser Ser

1 5 10 15

Ile Leu Gly Phe Ser Ser Ala Ser Ala Asn Ser His Leu Ser Asp Asp

20 25 30

Ser Pro Cys Val Ala Arg Ser Pro Thr Ser Gly Leu Tyr Tyr Asp Leu

35 40 45

Asn Ala Ile Ser Leu Ala Pro Pro Glu Trp Lys Asn Gly Lys Lys Val

50 55 60

Asp Gln Glu Ala Arg Asp Glu Ser Trp His Ala Lys Gly His Asp Tyr

65 70 75 80

Pro Ala Asn Phe Thr Ile Asn Val Cys Ala Pro Val Leu Glu Asn Val

85 90 95

Thr Asn Val Val Gly Val Asp Ala Ser Arg Trp Ala Asn Val Ser Ala

100 105 110

Phe Tyr Glu Gln Ala Gly Lys Ile Tyr Ser Met Gly Glu Gln Ala Ser

115 120 125

Glu Pro Phe Phe Arg Gly Arg Lys Leu Val Leu Asn Tyr Thr Asp Gly

130 135 140

Ser Pro Cys Pro Gly Asp Ser Asn Thr Ala Ser Gly Asn Ser Ser Ile

145 150 155 160

Arg Thr Lys Ser Thr Leu Met Ser Phe Leu Cys Asp Arg Ala Ala Glu

165 170 175

Phe Pro Gly Leu Glu Lys Leu Gly Ser Thr Gly Ser Arg

180 185

<210> 252

<211> 393

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 252

Met Asn Gly Phe Asn Glu Lys Gly Leu Asp Gly Asp Ala Phe Gly Glu

1 5 10 15

Lys Ser Asn Leu Ser Gly Leu Lys Thr Phe Asp Ala Phe Pro Lys Thr

20 25 30

Lys Thr Ser Tyr Thr Thr Pro Thr Arg Arg Gly Gly Gln Trp Thr Val

35 40 45

Leu Ile Leu Ala Val Cys Thr Leu Phe Ser Leu His Glu Leu Arg Thr

50 55 60

Trp Trp Arg Gly Thr Glu Ala His His Phe Ser Val Glu Lys Gly Val

65 70 75 80

Ser His Asp Leu Gln Leu Asn Leu Asp Met Val Val His Met Pro Cys

85 90 95

Asp Thr Leu Arg Ile Asn Ile Gln Asp Ala Ser Gly Asp Arg Val Leu

100 105 110

Ala Gly Glu Leu Leu Thr Arg Glu Asp Thr Asn Trp Asp Leu Trp Met

115 120 125

Lys Lys Arg Asn Phe Glu Ser His Gly Glu His Glu Tyr Gln Thr Leu

130 135 140

Asn His Glu Ala Ala Asp Arg Leu Ser Ala Gln Asp Glu Asp Ala His

145 150 155 160

Val His His Val Leu Gly Glu Val Arg Arg Asn Pro Arg Arg Lys Phe

165 170 175

Ser Lys Gly Pro Arg Leu Arg Trp Gly Asp Asn Lys Asp Ser Cys Arg

180 185 190

Ile Tyr Gly Ser Leu Glu Gly Asn Lys Val Gln Gly Asp Phe His Ile

195 200 205

Thr Ala Arg Gly His Gly Tyr Met Glu Leu Ala Pro His Leu Asp His

210 215 220

Glu Val Phe Asn Phe Ser His Met Ile Thr Glu Leu Ser Phe Gly Pro

225 230 235 240

His Tyr Pro Ser Leu Leu Asn Pro Leu Asp Lys Thr Ile Ala Glu Ser

245 250 255

Glu Thr His Tyr Gln Lys Phe Gln Tyr Phe Leu Ser Val Val Pro Thr

260 265 270

Leu Tyr Ser Lys Gly His Asn Ala Leu Asp Leu Val Thr Thr Asn Lys

275 280 285

Asp Asn Ser Val Arg Tyr Gly Arg Asn Thr Ile Phe Thr Asn Gln Tyr

290 295 300

Ala Ala Thr Ser Gln Ser Thr Ala Leu Pro Glu Ile Pro Thr Leu Ile

305 310 315 320

Pro Gly Ile Phe Phe Lys Tyr Asn Ile Glu Pro Ile Leu Leu Leu Val

325 330 335

Ser Glu Glu Arg Thr Gly Phe Leu Ala Leu Val Ile Arg Val Ile Asn

340 345 350

Thr Val Ser Gly Val Leu Val Thr Gly Gly Trp Ile Tyr Gln Ile Ser

355 360 365

Gly Trp Ile Val Glu Ile Leu Gly Lys Arg Lys Arg Gln Ser Glu Gly

370 375 380

Val Leu Thr Gly Lys His Tyr Ser Asp

385 390

<210> 253

<211> 292

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 253

Met Phe Thr Arg Thr Leu Arg Pro Ala Val Ala Val Ala Arg Thr Gln

1 5 10 15

Ala Val Gln Gln Gln Gln Ala Gly Met Ala Thr Leu Lys Glu Ile Asp

20 25 30

Gln Arg Leu Lys Ser Val Lys Asn Ile Gly Lys Ile Thr Lys Ser Met

35 40 45

Lys Val Val Ala Ser Thr Lys Leu Thr Arg Ala Glu Lys Ala Met Arg

50 55 60

Glu Ala Lys Lys Tyr Gly Ala Ala Asn Asn Val Leu Phe Glu Gln Thr

65 70 75 80

Lys Ala Gly Glu Glu Glu Pro Lys Glu Arg Lys Ile Leu Tyr Leu Ala

85 90 95

Met Thr Ser Asp Gly Gly Leu Cys Gly Gly Ile His Ser Asn Ile Thr

100 105 110

Arg Tyr Met Lys Lys Ala Val Ala Lys Glu Pro Gly Met Leu Ala Val

115 120 125

Val Gly Asp Lys Pro Lys Ala Gln Leu Ser Arg Ala Met Pro Lys Ala

130 135 140

Leu Thr Met Ser Phe Asn Gly Val Gly Lys Asp Val Pro Thr Phe Val

145 150 155 160

Glu Ala Ser Ala Ile Ala Asp Glu Ile Met Lys Ser Ala Gly Asp Phe

165 170 175

Asp Glu Ile Arg Ile Val Ser Asn Lys Tyr Leu Ser Ala Ile Ala Tyr

180 185 190

Glu Pro His Thr Asn Ala Val Ile Ser Ala Glu Ala Leu Arg Gln Ala

195 200 205

Ala Gly Phe Gln Gln Tyr Glu Met Glu Glu Asp Val Ser Lys Asp Leu

210 215 220

Ala Glu Phe Ala Leu Ala Asn Ala Ile Tyr Thr Ala Leu Val Glu Gly

225 230 235 240

His Ala Ala Glu Ile Ser Ala Arg Arg Gln Ala Met Glu Asn Ala Ser

245 250 255

Asn Asn Ala Asn Asp Met Ile Asn Ser Leu Gln Leu Gln Tyr Asn Arg

260 265 270

Gly Arg Gln Ala Val Ile Thr Thr Glu Leu Ile Asp Ile Ile Thr Gly

275 280 285

Ala Ser Ala Leu

290

<210> 254

<211> 317

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 254

Met Ser Phe Ala Ala Cys His Phe Cys Cys Phe Val His Leu Val Tyr

1 5 10 15

Thr Arg Leu Gln Ser Arg Gly Thr Gly Asn Asp Ile Asp Gln Leu Gly

20 25 30

Gly Asn Asp Ser Leu Ser Thr Thr Val Val Leu Gln Leu Glu Arg Val

35 40 45

Asp His Val Val Gly Val Val Gly Ser Val Leu His Ser Leu Pro Pro

50 55 60

Cys Arg Asp Leu Gly Gly Val Ser Leu Asp Gln Gly Ser Val Asp Gly

65 70 75 80

Val Gly Lys Ser Glu Leu Gly Gln Val Leu Gly Asp Ile Leu Leu His

85 90 95

Leu Val Leu Leu Glu Thr Gly Gly Leu Ser Glu Cys Leu Ser Gly Asp

100 105 110

Asp Gly Val Gly Val Arg Phe Val Gly Asp Ser Gly Lys Val Leu Val

115 120 125

Arg Asp Asp Ser Asp Leu Val Lys Val Thr Gly Arg Phe His Asn Leu

130 135 140

Ile Gly Asp Ser Ala Gly Leu Asp Glu Ser Gly Asp Ile Leu Ala Asp

145 150 155 160

Ala Val Glu Arg His Gly Gln Ser Leu Gly His Arg Ser Arg Glu Leu

165 170 175

Ser Leu Gly Leu Val Thr Asp Asn Ser Gln His Ser Gly Phe Leu Gly

180 185 190

His Ser Leu Leu His Val Ser Arg Asn Val Gly Val Asp Thr Thr Ala

195 200 205

Gln Thr Thr Val Gly Cys His Gly Glu Val Glu Asp Leu Ala Leu Leu

210 215 220

Gly Leu Leu Leu Thr Ser Leu Gly Leu Leu Glu Gln Asn Val Val Gly

225 230 235 240

Gly Thr Val Leu Leu Gly Phe Thr His Gly Leu Leu Ser Ser Arg Gln

245 250 255

Leu Gly Arg Gly Asn Asp Leu His Arg Leu Gly Asp Leu Pro Asn Val

260 265 270

Leu Asp Gly Phe Gln Thr Leu Val Asp Phe Leu Gln Cys Gly His Thr

275 280 285

Gly Leu Leu Leu Leu Asp Ser Leu Ser Pro Gly Asp Arg His Gly Arg

290 295 300

Ser Glu Ser Thr Arg Glu His Gly Val Glu Arg Gly Glu

305 310 315

<210> 255

<211> 311

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 255

Met Glu Asn Leu Leu Arg Gln Met Gln Gly Gly Gly Gly Arg Met Gly

1 5 10 15

Ala Arg Pro Gly Pro Gly Gly Glu Thr Ile Leu Ala Asp Asn Gly Glu

20 25 30

Thr Val His Ile Ser Ser Leu Ala Leu Leu Lys Met Leu Lys His Gly

35 40 45

Arg Ala Gly Val Pro Met Glu Val Met Gly Leu Met Leu Gly Glu Phe

50 55 60

Val Asp Asp Tyr Thr Ile Ser Cys Val Asp Val Phe Ala Met Pro Gln

65 70 75 80

Ser Gly Thr Thr Val Thr Val Glu Ser Val Asp His Val Phe Gln Thr

85 90 95

Lys Met Leu Asp Met Leu Lys Gln Thr Gly Arg Pro Glu Met Val Val

100 105 110

Gly Trp Tyr His Ser His Pro Gly Phe Gly Cys Trp Leu Ser Ser Val

115 120 125

Asp Val Asn Thr Gln Gln Ser Phe Glu Gln Leu His Pro Arg Ala Val

130 135 140

Ala Val Val Ile Asp Pro Ile Gln Ser Val Arg Gly Lys Val Val Ile

145 150 155 160

Asp Ala Phe Arg Ser Ile Asn Pro Gln Ser Leu Val Ala Gly Gln Glu

165 170 175

Ser Arg Gln Thr Thr Ser Asn Ile Gly His Leu Asn Lys Pro Ser Ile

180 185 190

Gln Ala Leu Ile His Gly Leu Asn Arg His Tyr Tyr Ser Leu Ala Ile

195 200 205

Asp Tyr Arg Lys Thr Glu Gly Glu Gln Gly Met Leu Leu Asn Leu His

210 215 220

Lys Arg Gly Trp Thr Glu Gly Leu Lys Met Arg Asp His Ser Glu Met

225 230 235 240

Lys Glu Gly Asn Glu Lys Ala Ile Lys Glu Met Leu Ser Leu Ala Ser

245 250 255

Ala Tyr Thr Lys Ser Val Gln Glu Glu Thr Thr Met Thr Ala Glu Gln

260 265 270

Leu Lys Thr Arg His Val Gly Lys Leu Asp Pro Lys Arg His Leu Gly

275 280 285

Glu Ala Ala Glu Lys Ala Met Gly Asp Gln Val Thr Gln Ser Leu Ala

290 295 300

Met Gly Val Leu Ala Glu Leu

305 310

<210> 256

<211> 93

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 256

Met Gly Ala Ile Pro Glu Tyr Asp Pro Glu Glu Pro Leu Glu Thr Lys

1 5 10 15

Pro Phe Lys Phe Val Thr Ala Gly Tyr Asp Ala Arg Phe Pro Gln Gln

20 25 30

Asn Gln Thr Lys His Cys Trp Gln Asn Tyr Val Asp Tyr Tyr Lys Cys

35 40 45

Val Glu Ala Lys Gly Glu Asp Phe Arg Pro Cys Lys Gln Phe Tyr His

50 55 60

Ala Phe Arg Ser Leu Cys Pro Lys Ala Trp Thr Asp Arg Trp Asp Thr

65 70 75 80

Gln Arg Glu Gly Gly Asn Phe Pro Ala Ile Leu Asn Lys

85 90

<210> 257

<211> 252

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 257

Gly Pro Leu Leu Glu Glu Leu Asp Val Glu Ala Tyr Ala Lys Lys Tyr

1 5 10 15

Arg Tyr Leu Arg Phe Met Cys Gln Glu Thr Leu Asp His Leu Ala Phe

20 25 30

Leu Lys Asp Lys Val Lys Asp Val Glu Gly Phe Trp Ala Ser Thr Leu

35 40 45

Leu Lys His Arg Asp Leu Arg Gly Tyr Ile Thr Ser Arg Ser Asp Lys

50 55 60

Asp Ala Leu Lys Tyr Leu Thr His Ile Glu Leu Val Gln Asp Pro Lys

65 70 75 80

Asp Pro Arg Pro Phe Ala Leu Lys Phe Tyr Phe Lys Glu Asn Pro Tyr

85 90 95

Phe Ser Asp Leu Val Leu Glu Lys Lys Tyr Asp Met Ser Glu Gly Ser

100 105 110

Glu Pro Ala Pro Ala Asp Gly Ser Ile Thr Glu Gly Met Arg Asn Phe

115 120 125

Lys Glu Asp Glu Leu Val Thr Lys Ala Thr Thr Ile Asn Trp Lys Ser

130 135 140

Asp Asp Lys Asn Leu Val Ala Lys Gln Pro Arg Ser Lys Ile Pro Asp

145 150 155 160

Asn Asp Asp Asp Glu Asp Phe Asp Gly Asp Val Gly Ser Phe Phe Asn

165 170 175

Tyr Phe Thr Asp Asp Thr Asp Ile Phe Gln Ile Gly Ala Leu Leu Gln

180 185 190

Ser Glu Leu Leu Pro Asp Ala Ile Asp Tyr Phe Val Gly Arg Gly Glu

195 200 205

Gln Val Asp Ser Glu Gly Glu Glu Leu Asp Glu Leu Glu Glu Asp Asp

210 215 220

Glu Asp Asp Asp Glu Asp Asp Glu Gly Ser Ile Asp Leu Glu Asp Glu

225 230 235 240

Glu Glu Gln Pro Ser Lys Lys Lys Pro Lys Arg Ala

245 250

<210> 258

<211> 165

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 258

Gly Ser Phe Leu Thr Pro Gln His Thr His Thr Pro Phe Gly Arg Ala

1 5 10 15

Met His Asn Ala Leu Thr Val Ser Arg Leu Asn Asp Lys Phe Gln Glu

20 25 30

Pro Leu Val Val Leu Val Glu Leu Leu Arg Ala Arg Val Leu His Asp

35 40 45

Arg Asn Phe Ser Asn Arg Gln Phe Ser Gly Gly Pro Ser Phe Gly Thr

50 55 60

Asp Asn Gln Lys Arg Ser Met Leu Leu Ile Phe Arg Thr Leu Ser Ile

65 70 75 80

Ile Pro Leu Gln Phe Lys Ala Glu His Trp Ser Gly Pro Leu Ser Arg

85 90 95

Glu Leu Leu Val Phe Asn Ser Phe His Lys Thr Leu Ser Arg Ser Leu

100 105 110

Arg Thr Leu Val Glu Ser Ile Thr Met Asn Ala Phe Leu Lys Asn Asn

115 120 125

Ala Arg Arg Ala Arg Asp Asp Tyr Leu Asp Ile Ala Leu Ser Leu Pro

130 135 140

Phe Gln Asn Asp Thr Asn Thr Gly Phe Gly Ile Phe Phe Lys Val Gly

145 150 155 160

Gly Leu Ala Gly Val

165

<210> 259

<211> 94

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 259

Gln Ile Tyr Leu Asp Ala Leu Thr Thr Phe Ala Glu Gly Asn Ile Thr

1 5 10 15

Glu Glu Asn Lys Asp Ser Glu Ser Val Lys Glu Ala Lys Gln Ser Ala

20 25 30

Met Glu Ile Leu Gly Asp Ala Ile Pro Asn Val Lys Asp Pro Glu Ala

35 40 45

Glu Leu Leu Arg Gly Phe Arg Phe Trp Asp Ala Val Leu Val Cys Val

50 55 60

Arg Thr Leu Lys Ala Asp Arg Ala Ile Asp Leu Lys Leu Ala Glu Ser

65 70 75 80

Phe Glu Ala Ala Asn Ser Tyr Leu Asn Met Met Arg Pro Asn

85 90

<210> 260

<211> 251

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 260

Gly Ser Phe Leu Thr Pro Gln His Thr His Thr Pro Phe Gly Arg Ala

1 5 10 15

Met His Asn Ala Leu Thr Val Ser Arg Leu Asn Asp Lys Phe Gln Glu

20 25 30

Pro Leu Val Val Leu Val Glu Leu Leu Arg Ala Arg Val Leu His Asp

35 40 45

Arg Asn Phe Ser Asn Arg Gln Phe Ser Gly Gly Pro Ser Phe Gly Thr

50 55 60

Asp Asn Gln Lys Arg Ser Met Leu Leu Ile Phe Arg Thr Leu Ser Ile

65 70 75 80

Ile Pro Leu Gln Phe Lys Ala Glu His Trp Ser Gly Pro Leu Ser Arg

85 90 95

Glu Leu Leu Val Phe Asn Ser Phe His Lys Thr Leu Ser Arg Ser Leu

100 105 110

Arg Thr Leu Val Glu Ser Ile Thr Met Asn Ala Phe Leu Lys Asn Asn

115 120 125

Ala Arg Arg Ala Arg Asp Asp Tyr Leu Asp Ile Ala Leu Ser Leu Pro

130 135 140

Phe Gln Asn Asp Thr Asn Thr Gly Phe Gly Ile Phe Phe Lys Ile Tyr

145 150 155 160

Leu Asp Ala Leu Thr Thr Phe Ala Glu Gly Asn Ile Thr Glu Glu Asn

165 170 175

Lys Asp Ser Glu Ser Val Lys Glu Ala Lys Gln Ser Ala Met Glu Ile

180 185 190

Leu Gly Asp Ala Ile Pro Asn Val Lys Asp Pro Glu Ala Glu Leu Leu

195 200 205

Arg Gly Phe Arg Phe Trp Asp Ala Val Leu Val Cys Val Arg Thr Leu

210 215 220

Lys Ala Asp Arg Ala Ile Asp Leu Lys Leu Ala Glu Ser Phe Glu Ala

225 230 235 240

Ala Asn Ser Tyr Leu Asn Met Met Arg Pro Asn

245 250

<210> 261

<211> 148

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 261

Gly Ile Tyr Leu Asp Gly Asn Asn Asp Leu Val Thr Met Lys Gly Asn

1 5 10 15

Tyr Ile Tyr His Thr Ser Gly Arg Ser Pro Lys Val Gln Gly Asn Thr

20 25 30

Leu Leu His Ala Val Asn Asn Tyr Trp His Asp Asn Ser Gly His Ala

35 40 45

Phe Glu Ile Gly Glu Gly Gly Tyr Val Leu Ala Glu Gly Asn Val Phe

50 55 60

Gln Asp Val Thr Thr Pro Val Glu Asp Pro Val Asp Gly Gln Leu Leu

65 70 75 80

Thr Ser Pro Asp Pro Ser Thr Asn Ala Gln Cys Ser Ser Tyr Leu Gly

85 90 95

Arg Ala Cys Glu Ile Asn Gly Phe Gly Asn Ser Gly Thr Phe Asn Gln

100 105 110

Ala Asp Thr Ser Leu Leu Ser Lys Phe Lys Gly Gln Asn Ile Ala Ser

115 120 125

Ala Asp Ala Tyr Ser Lys Val Ala Ser Ser Val Ala Ser Asn Ala Gly

130 135 140

Gln Gly His Leu

145

<210> 262

<211> 120

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 262

Val Arg Val Val Thr Phe Trp Pro Arg Val Thr Ser Ser Arg Met Leu

1 5 10 15

Leu Pro Pro Leu Arg Thr Pro Leu Thr Ala Ser Ser Ser Leu Pro Leu

20 25 30

Thr Pro Ala Pro Thr Leu Ser Ala Arg His Thr Leu Ala Gly Pro Ala

35 40 45

Lys Ser Thr Ala Ser Val Thr Leu Val Pro Ser Thr Arg Leu Thr Leu

50 55 60

Ala Cys Cys Leu Asn Leu Arg Val Arg Thr Leu Leu Leu Leu Met Leu

65 70 75 80

Thr Leu Arg Leu Pro Arg Ala Leu Pro Ala Thr Pro Val Arg Asp Thr

85 90 95

Cys Lys Met Glu Arg Gly Gly Ser Glu Leu Asn Leu Leu Met Ser Asp

100 105 110

Asp Ile Ala Leu Ala Ala Cys Trp

115 120

<210> 263

<211> 160

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 263

Met Ser Ala Gln Pro Leu Arg Ile Val Met Ala Cys Asp Glu Ala Gly

1 5 10 15

Val Pro Tyr Lys Asp Ala Ile Lys Ala Val Leu Glu Lys Ser Pro Leu

20 25 30

Val Ala Ser Val Ser Asp Val Gly Val Asn Asp Ala Ser Asp Lys Thr

35 40 45

Ala Tyr Pro His Pro Ala Val Glu Gly Ala Gln Gln Ile Lys Ala Gly

50 55 60

Lys Ala Asp Arg Gly Leu Phe Ile Cys Gly Thr Gly Leu Gly Val Ala

65 70 75 80

Ile Ala Ala Asn Lys Val Pro Gly Ile Arg Ala Val Thr Ala His Asp

85 90 95

Pro Phe Ser Val Glu Arg Ser Ile Leu Ser Asn Asp Ala Gln Val Leu

100 105 110

Cys Met Gly Gln Arg Val Ile Gly Val Glu Leu Ala Lys Lys Leu Ala

115 120 125

Leu Asp Trp Leu Asn Tyr Arg Phe Asp Pro Lys Ser Ala Ser Ala Ala

130 135 140

Lys Val Gln Ala Ile Ser Asp Tyr Glu Thr Lys Phe Ala Gly Ser Ser

145 150 155 160

<210> 264

<211> 150

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 264

Met Ser Val Thr Thr Thr Ser Ser Ala Ala Ala Ala Ser Cys Thr Pro

1 5 10 15

Ser Trp Gln Ile Pro Val Asp Asp Val Ala Cys Ala Gly Gln Ile Ser

20 25 30

Gly Asn Ile Thr Lys Val Phe Asp Thr Cys Cys Lys Gly Asn Ser Pro

35 40 45

Val Lys Tyr Asn Asp Asp Cys Asn Ile Tyr Cys Leu Ala Gln Gly Gln

50 55 60

Thr Lys Gln Glu Leu Thr Asp Cys Leu Thr Glu Lys Ser Gly Asn Asn

65 70 75 80

Gln Ile Phe Cys Gly His Gly Lys Gln Asn Ala Thr Ala Thr Ala Glu

85 90 95

Ala Thr Thr Thr Lys Glu Thr Gly Thr Ser Thr Gly Thr Ser Thr Ser

100 105 110

Ser Thr Gly Thr Ser Thr Glu Thr Asn Ala Ala Val Leu Asn Gln Pro

115 120 125

Ile Ser Lys Thr Gly Leu Gly Leu Val Ala Met Leu Phe Cys Ser Ala

130 135 140

Leu Val Gly Val Val Ala

145 150

<210> 265

<211> 197

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 265

Ala Gly Val Asp Arg Gly Val Ile Thr Lys Asp Glu Lys Asp Ser Ser

1 5 10 15

Ile Asn Arg Leu Leu Val Thr Gly Tyr Gly Leu Ala Glu Val Met Gly

20 25 30

Thr Asp Gly Val Asn Gly Ile Lys Thr Arg Thr Asn His Val Met Glu

35 40 45

Thr Cys Glu Val Leu Gly Ile Glu Ala Ala Arg Gln Thr Ile Tyr Asn

50 55 60

Glu Ile Gln His Thr Met Thr Ser His Gly Met Ser Ile Asp Pro Arg

65 70 75 80

His Val Met Leu Leu Gly Asp Val Met Thr Tyr Lys Gly Glu Val Leu

85 90 95

Gly Ile Thr Arg Phe Gly Val Gln Lys Met Lys Asp Ser Val Leu Met

100 105 110

Leu Ala Ser Phe Glu Lys Thr Thr Asp His Leu Phe Asp Ala Ser Leu

115 120 125

Phe Ser Lys Lys Asp Glu Ile Gln Gly Val Ser Glu Cys Ile Ile Met

130 135 140

Gly Thr Pro Ala Pro Gly Cys Gly Thr Ser Leu Ala Ser Ile Val Thr

145 150 155 160

Pro Ala Pro Leu Leu Pro Arg Lys Lys Pro Leu Leu Phe Glu Thr Ala

165 170 175

Phe Lys Ala Gly Gln Asp Arg Leu Ser Tyr His Glu Asn Asn Gly Gly

180 185 190

Met Glu Val Asp Met

195

<210> 266

<211> 249

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 266

Ala Gly Ser Gly Gly Ala Tyr Ser Phe Ser Leu Thr Thr Phe Ser Pro

1 5 10 15

Ser Gly Lys Leu Val Gln Ile Glu His Ala Leu Ala Ala Val Ala Gly

20 25 30

Gly Thr Thr Ser Leu Gly Ile Lys Ala Thr Asn Gly Val Val Leu Ala

35 40 45

Thr Glu Lys Lys Ser Pro Ser Leu Leu Leu Asp Thr Ser Val Leu Glu

50 55 60

Lys Val Ala Pro Ile Cys Pro Asn Ile Gly Phe Val Tyr Ser Gly Met

65 70 75 80

Gly Pro Asp Phe Arg Val Leu Val Ala Lys Ala Arg Lys Ile Ala Gln

85 90 95

Ala Tyr Tyr Lys Val Tyr Gly Glu Tyr Pro Pro Thr Lys Val Leu Val

100 105 110

Gln Glu Val Ala Gly Val Met Gln Lys Ala Thr Gln Ser Gly Gly Val

115 120 125

Arg Pro Tyr Gly Ile Ser Leu Leu Ile Ala Gly Trp Asp Ser His Arg

130 135 140

Gly Gln Ser Leu Tyr Gln Val Asp Pro Ser Gly Ser Tyr Trp Ala Trp

145 150 155 160

Lys Ala Ser Ala Ile Gly Lys Asn Met Val Asn Gly Lys Thr Phe Leu

165 170 175

Glu Lys Arg Tyr Asn Asp Asp Leu Ser Leu Glu Asp Ala Ile His Thr

180 185 190

Ala Leu Leu Thr Leu Lys Glu Gly Phe Glu Gly Gln Met Thr Glu Asn

195 200 205

Thr Ile Glu Ile Gly Val Val Thr Val Pro Thr Ala Glu Gln Met Gln

210 215 220

Glu Lys Pro Gly Glu Arg Leu Pro Pro Thr Phe Arg Lys Leu Thr Glu

225 230 235 240

Gln Glu Val Arg Asp Tyr Leu Ala Leu

245

<210> 267

<211> 346

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 267

Met Leu Thr Ser Ala Phe Ser Thr Ser Ala Ser Lys Met Leu Gly Lys

1 5 10 15

Arg Ala Val Ser Ser Ser Ser Ala Leu Asn Gly Lys Val Ala Val Leu

20 25 30

Gly Ala Ala Gly Gly Ile Gly Gln Pro Leu Ser Leu Leu Val Lys Gln

35 40 45

Asn Pro Ala Val Ser Ser Leu Ser Leu Tyr Asp Val Arg Gly Ser Pro

50 55 60

Gly Val Ala Ala Asp Ile Ser His Ile Asn Thr Pro Ala Val Thr Glu

65 70 75 80

Gly Phe Leu Pro Asp Asn Asp Gly Leu Lys Gln Ala Leu Glu Gly Ala

85 90 95

Glu Val Val Leu Ile Pro Ala Gly Val Pro Arg Lys Pro Gly Met Thr

100 105 110

Arg Asp Asp Leu Phe Asn Thr Asn Ala Ser Ile Val Lys Met Leu Ala

115 120 125

Glu Ala Ser Ala Lys Tyr Cys Pro Lys Ala Met Met Leu Ile Ile Ala

130 135 140

Asn Pro Val Asn Ser Thr Val Pro Ile Val Ala Glu Thr Phe Lys Arg

145 150 155 160

Ala Gly Val Tyr Asp Pro Ala Arg Leu Phe Gly Val Thr Thr Leu Asp

165 170 175

Val Val Arg Ser Ser Thr Phe Val Ser Gly Ile Thr Gly Ala Lys Pro

180 185 190

Ser Asp Thr Val Val Gln Val Ile Gly Gly His Ser Gly Ala Thr Ile

195 200 205

Val Pro Leu Leu Ser Gln Ile Pro Gln Gly Asp Lys Ile Val Lys Ala

210 215 220

Gly Gly Gln Gln Tyr Ala Asp Leu Val Lys Arg Ile Gln Phe Gly Gly

225 230 235 240

Asp Glu Val Val Lys Ala Lys Asp Gly Thr Gly Ser Ala Thr Leu Ser

245 250 255

Met Ala Tyr Ala Ala Ala Val Phe Asn Asp Ala Leu Leu Lys Ala Met

260 265 270

Asp Gly Gln Lys Gly Leu Val Gln Pro Ala Tyr Val Glu Ser Pro His

275 280 285

Phe Ala Lys Glu Gly Ala Lys Tyr Phe Ala Ser Asn Val Glu Leu Gly

290 295 300

Pro Asn Gly Val Glu Lys Ile Leu Asp Ile Gly Asn Met Ser Ser Glu

305 310 315 320

Glu Gln Glu Leu Leu Lys Glu Cys Leu Pro Gln Leu Ala Lys Asn Ile

325 330 335

Ala Ala Gly Glu Lys Phe Val Ala Asp Asn

340 345

<210> 268

<211> 373

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 268

Met Ser Leu Phe Leu Leu Phe Ser Leu Ala Leu Ile Ile Ile Gly Ser

1 5 10 15

Asn Val Val Gly Tyr Pro Leu Val Val Ser Asp Glu Leu Leu Thr Ser

20 25 30

Ser Asn Val Leu Gly Glu Leu Gly Lys Ala Leu Leu Lys Glu Leu Leu

35 40 45

Leu Leu Arg Gly His Val Ala Asp Val Glu Asp Leu Leu Asn Thr Val

50 55 60

Gly Ala Glu Leu Asp Val Gly Gly Glu Val Leu Ser Thr Leu Leu Gly

65 70 75 80

Glu Val Gly Ala Leu Asp Val Ser Gly Leu Asn Glu Thr Leu Leu Ala

85 90 95

Val His Ser Leu Glu Glu Ser Val Val Glu Asp Gly Ser Gly Val Ser

100 105 110

His Gly Glu Gly Ser Gly Ala Ser Ala Val Leu Gly Leu Asp Asp Phe

115 120 125

Val Thr Ala Lys Leu Asp Ala Leu Asp Glu Val Ser Val Leu Leu Ala

130 135 140

Ala Ser Leu Asp Asn Leu Val Ala Leu Arg Asp Leu Gly Glu Gln Gly

145 150 155 160

His Asp Gly Gly Ala Arg Val Thr Thr Asp Asp Leu Asp His Gly Val

165 170 175

Gly Gly Leu Gly Thr Gly Asp Ala Arg Asp Glu Ser Gly Arg Ala Asp

180 185 190

Asn Val Glu Gly Gly Asp Thr Glu Glu Thr Gly Arg Val Val Asp Thr

195 200 205

Ser Thr Leu Glu Gly Leu Ser Asp Asp Arg His Gly Gly Val Asp Gly

210 215 220

Val Gly Asn Asp Glu His His Ser Leu Gly Ala Val Leu Gly Arg Ser

225 230 235 240

Leu Ser Lys His Leu Asp Asp Gly Ser Val Gly Val Glu Lys Val Val

245 250 255

Thr Gly His Ala Gly Leu Ala Arg Asn Thr Ser Arg Asn Glu Asp His

260 265 270

Leu Ser Thr Leu Glu Gly Leu Leu Glu Ala Ile Val Val Gly Glu Glu

275 280 285

Ala Leu Gly Asp Ser Arg Gly Val Asp Val Ala Asn Val Ser Ser Asn

290 295 300

Thr Arg Gly Ala Ala Asn Ile Val Lys Gly Glu Ala Gly Asp Ser Arg

305 310 315 320

Val Leu Leu Asp Gln Gln Gly Glu Gly Leu Ala Asn Thr Ala Ser Ser

325 330 335

Thr Glu Asp Gly Asn Leu Ser Val Gln Gly Ala Gly Arg Arg Asp Cys

340 345 350

Ser Leu Ala Glu His Leu Gly Ser Arg Cys Arg Lys Gly Ala Gly Gln

355 360 365

His Phe Gly Ser Lys

370

<210> 269

<211> 435

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 269

Gly Ala Ser Val Leu Leu Pro Ala Leu His Phe Asp Glu Arg Glu Gln

1 5 10 15

Arg Pro Gly Arg Thr Ser Ser Thr Ala Ser Leu Leu Gln Arg His Asp

20 25 30

Arg Asn Glu Pro Leu Ser Leu Asn Ser His Ser Pro Thr Ser Val Asp

35 40 45

His Thr Pro Thr Thr Ala His Phe Thr Gly Ala Glu Glu Leu Leu Ala

50 55 60

Ser Asp Val Gly Pro Thr Ala Thr Ala Gly Leu Pro Gly Asp Ala Glu

65 70 75 80

Leu Glu Ser Lys Leu Lys Leu Leu Glu Glu Val Lys Arg Ala Arg Glu

85 90 95

Ser Val His Ser Ser Leu Glu Arg Ile Arg Ala Gly Thr Pro Thr Pro

100 105 110

Ser Ile Ser Gln Gly Met Pro Ser Pro Thr Pro Ser Gly Ala Pro Gly

115 120 125

Tyr Ala Arg Thr Pro Ser Ser Val Gly Leu Ser Asp Asp Val Arg Ser

130 135 140

Arg Arg Gly Ser Thr Thr Ser Ser Lys Val Leu Asp Ala Ile Asp Lys

145 150 155 160

Pro Arg Val Ala Thr Gln Ser Glu Trp Asp Glu Tyr Val Arg Asn Arg

165 170 175

His Val Ile Ser Pro Pro Pro Thr Gln Phe Ala Val Leu Pro Thr Ser

180 185 190

Ala Ala Met Val Asp Arg Gly Thr Ser Arg His Ser Gln Tyr Ala Leu

195 200 205

Val Ser Asp Gly Val Ala Lys Ala Leu Asp Arg Arg Glu Arg Thr Ile

210 215 220

Ser Met Met Glu Pro Gln Val Ala Glu Asp Trp Gly Pro Arg Glu Thr

225 230 235 240

Leu Asp Ser Thr Pro Ala His Val Ser Met Gly Arg Arg Ala Met Ser

245 250 255

Phe His Glu Ile Pro Leu Ala Ser Pro Val Ala Ala Ser Arg Pro Gln

260 265 270

Asp Arg Ser Ser Tyr Ser Ala Gly Pro Arg Gln Val Ile Gly Ser Ala

275 280 285

Ala Gly His Thr Gln Arg Pro Gly Ile Ser Gln Ser Arg Ser Ala His

290 295 300

Gly Arg Thr Met Thr Tyr Asp Glu Leu Thr Glu Arg His Arg Gln Arg

305 310 315 320

Leu Ser Ala Leu Gln Ala Pro Val Ser Ala Lys Ile Arg Glu Pro Met

325 330 335

Asp Ile Ala Ser Ala Lys Ala Ser Trp Asp Lys Gln Lys Arg Val Glu

340 345 350

Arg Asp Glu Met Lys Arg Arg Glu Ala Glu Lys Leu Ala Gln Ala His

355 360 365

Ala Arg Glu Arg Arg Gly Pro Ala Val Asp Lys Lys Glu Val Leu Lys

370 375 380

Ser Thr Asp Glu Trp Arg Arg Ser Val His Gly Gly Leu Asp Gly Phe

385 390 395 400

Ala Val Pro His Leu Pro Ala His Ala Arg Gly Ser Thr Gln Pro Gly

405 410 415

Gly Ser Gly Ala Lys Arg Ser Ser Leu Ser Gln Arg Pro Ser Asn Tyr

420 425 430

Phe Ala Asn

435

<210> 270

<211> 225

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 270

Gly Gln Ala Cys Cys Phe Arg His Cys Ile Ser Thr Ser Gly Ser Asn

1 5 10 15

Asp Leu Asp Val Arg Leu Arg Leu Gln Val Cys Cys Ser Val Thr Ile

20 25 30

Ala Met Asn Leu Tyr Arg Ser Thr Arg Thr Arg Arg His Leu Trp Thr

35 40 45

Thr Leu Pro Leu Leu Arg Ile Ser Leu Val Leu Lys Ser Cys Ser Pro

50 55 60

Pro Thr Ser Asp Arg Pro Arg Gln Leu Gly Tyr Pro Val Met Arg Ser

65 70 75 80

Leu Arg Ala Ser Ser Ser Cys Leu Lys Arg Ser Asn Val His Gly Asn

85 90 95

Arg Tyr Ile Ala Arg Ser Arg Gly Ser Glu Pro Ala Arg Leu Pro Arg

100 105 110

Leu Ser Ala Arg Glu Cys Pro Ala Arg His Pro Leu Val Pro Leu Val

115 120 125

Thr Leu Glu Leu Arg Arg Leu Ser Ala Cys Arg Thr Thr Cys Ala Arg

130 135 140

Asp Glu Ala Gln Arg Pro Ala Pro Arg Phe Leu Thr Leu Ser Thr Ser

145 150 155 160

Leu Glu Ser Leu Pro Asn Pro Asn Gly Thr Ser Thr Phe Ala Thr Gly

165 170 175

Met Ser Ser His Leu His Pro Leu Ser Leu Pro Tyr Cys Pro His Leu

180 185 190

Leu Arg Trp Ser Ile Val Val Pro Val Asp Thr Ala Ser Met Pro Leu

195 200 205

Phe Pro Thr Ala Leu Pro Lys Arg Leu Thr Gly Gly Ser Glu Leu Phe

210 215 220

Gln

225

<210> 271

<211> 168

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 271

Met Gly Ser Pro Ala Pro His His Arg His Gln Ser Ser Leu Glu Gly

1 5 10 15

Val Ile Asp Phe Ser Thr Gly Arg Gly His Pro Leu Asn Pro Tyr Gln

20 25 30

Arg Asp Lys Ala Glu Ser Val Phe Thr Gly Ile Ile Asn Arg Phe Glu

35 40 45

Asp Ser Ser Thr Val Glu Lys Pro Tyr Asn Arg Ala Lys Leu Val Arg

50 55 60

Leu Thr Tyr Glu Tyr Ala Arg Ser Glu Asp Ser Arg Cys Asn Phe Leu

65 70 75 80

Gln Ala Phe Phe Gly Ser Val Asn Val Thr Met Asp Asp Ser Ile Asp

85 90 95

Phe Asp Asp Glu Ala Val Glu Glu Gly Ile Arg Ser Ser Leu Asn Ser

100 105 110

Phe Ala Asp Phe Leu Val Glu Asn Phe Phe Leu Pro Leu Lys Ala Ser

115 120 125

Ala Ser Arg Thr Pro Pro Ala Pro Gln Pro Lys Phe Arg Ala Asp Val

130 135 140

Leu Leu Trp Gly Leu Trp Lys Glu Trp Pro Arg Ser Asp Ala Thr Ala

145 150 155 160

Ser Ser Ala Ile Asp Ile Val Ala

165

<210> 272

<211> 253

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 272

Gln Asp Ala Pro Ser Pro Pro Ala Gln Val Pro Ser Arg Arg Pro Ala

1 5 10 15

Val Gly Ser Val Glu Arg Val Ala Ser Leu Arg Arg Asp Cys Leu Ile

20 25 30

Arg Asp Arg His Arg Cys Val Ile Ser Arg Asn Phe Asp Met Lys Glu

35 40 45

Ala Glu Arg Arg Leu Asp Asp Ser Gly Tyr Asp His Ala Ser Asp Asp

50 55 60

Glu Gly His Leu Leu Lys Asp Gln Glu His Gly Ser Phe Ala Glu Leu

65 70 75 80

Glu Val Ala His Ile Leu Pro His Ser Leu Met Thr Thr Thr Ala Asn

85 90 95

Ser Glu Leu Asn Lys Ser Lys Glu Thr Ala Leu Thr Ile Leu Asn Met

100 105 110

Phe Asp Ser Gly Ile Val His Leu Ile Asp Gly Pro Asp Ile Asp Arg

115 120 125

Pro Arg Asn Ala Leu Thr Leu Ser Ile Asp Leu His Arg Gln Phe Gly

130 135 140

Asn Phe Lys Val Phe Phe Glu Pro Met Pro Glu Pro His Thr Tyr Arg

145 150 155 160

Ile Asp Ser Thr Leu Arg Gln Pro Phe Arg Asn Pro Ile Phe Pro Val

165 170 175

Thr Arg Ala Leu Tyr Leu Thr Pro Glu Arg Thr Ile Asp Pro Pro Ser

180 185 190

Gly Arg Leu Leu Ala Val His Arg Ala Ile Cys His Ile Leu His Leu

195 200 205

Ser Ala Ala Gly Asn Tyr Ile Asp Ser Ile Leu Arg Asp Met Asp Asp

210 215 220

Gly Thr Val Gln Ala Asn Gly Ser Thr Arg Leu Ala Ser Ile Val Arg

225 230 235 240

Leu Lys Leu Gly Gly Trp Trp Asp Gly Thr Val Val Gly

245 250

<210> 273

<211> 106

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 273

Met Val Asn Ile Pro Lys Thr Arg Arg Thr Tyr Cys Lys Gly Lys Glu

1 5 10 15

Cys Lys Lys His Thr Gln His Lys Val Thr Gln Tyr Lys Ala Gly Lys

20 25 30

Ala Ser Leu Phe Ala Gln Gly Lys Arg Arg Tyr Asp Arg Lys Gln Ser

35 40 45

Gly Tyr Gly Gly Gln Thr Lys Pro Val Phe His Lys Lys Ala Lys Thr

50 55 60

Thr Lys Lys Val Val Leu Arg Leu Glu Cys Thr Ser Cys Lys Thr Lys

65 70 75 80

Ala Gln Leu Ala Leu Lys Arg Cys Lys His Phe Glu Leu Gly Gly Asp

85 90 95

Lys Lys Thr Lys Gly Ala Ala Leu Val Phe

100 105

<210> 274

<211> 84

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 274

Met Ile Leu Glu Gly Ser Glu Ser Lys Leu Arg Phe Ser Tyr Asp Arg

1 5 10 15

Pro Thr Arg Lys Asp Met Ser Arg His Ile Met Ala Cys Ala Gln His

20 25 30

Ser Ser Arg Val His Gln Arg Cys Pro Thr Leu Pro Leu Arg Cys Val

35 40 45

Gly Cys Asp Ser Ser Thr Thr Ala Phe Gln Tyr His Leu Phe Ala Cys

50 55 60

Arg Ser Glu Asp Asp Phe Thr Thr Val His Ile Thr Asn Arg Lys Cys

65 70 75 80

Gln Gln Asp Gln

<210> 275

<211> 41

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 275

Met Lys Leu Thr Phe Lys Asp Leu Lys Gln Glu Lys Phe Val Ile Glu

1 5 10 15

Val Glu Pro Ser Glu Thr Val Arg Glu Val Lys Gln Lys Leu Leu Lys

20 25 30

Lys Lys Ala Asn Met Arg Arg Asn Glu

35 40

<210> 276

<211> 369

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 276

Gly Pro Glu Ala Gly Glu Val Arg Asn Arg Gly Arg Ala Leu Arg Asp

1 5 10 15

Cys Ser Arg Ser Gln Ala Lys Ile Ala Gln Glu Lys Gly Glu Tyr Glu

20 25 30

Ala Glu Arg Met Lys Val Ile Tyr Ser Gly Lys Ile Leu Gln Asp Asp

35 40 45

Lys Thr Val Glu Ser Tyr Asn Ile Gln Glu Lys Asp Phe Leu Val Cys

50 55 60

Leu Pro Ser Lys Gly Pro Lys Pro Ala Ala Ser Ser Ser Ala Ser Gln

65 70 75 80

Ala Pro Ala Thr Pro Ala Pro Arg Ala Pro Val Ala Thr Pro Ala Ala

85 90 95

Pro Ala Pro Ala Ala Pro Ala Pro Ala Ser Ser Thr Pro Ala Val Pro

100 105 110

Ala Thr Pro Ser Pro Ala Gly Ala Gln Thr Gly Pro Ser Phe Gly Asp

115 120 125

Pro Ser Ala Leu Thr Met Gly Ser Ala Ala Glu Gly Ala Val Thr Gln

130 135 140

Met Glu Ala Met Gly Phe Ala Arg Ser Asp Ile Asp Arg Ala Met Arg

145 150 155 160

Ala Ala Phe Phe Asn Pro Asp Arg Ala Val Asp Tyr Leu Leu Asn Gly

165 170 175

Ile Pro Ala Asp Val Gln Gln Glu Gln Gln Gln Arg Gln Gln Glu Gln

180 185 190

Gln Ala Asp Arg Ala Ala Glu Gln Ala Pro Val Pro Ser Ala Glu Asp

195 200 205

Ala Ala Ala Ala Ala Ala Leu Gly Gly Asp Glu Gly Phe Asn Met Phe

210 215 220

Glu Ala Ala Ala Gln Ala Gly Asp Gly Arg Gly Gly Gly Ala Arg Ser

225 230 235 240

Gly Gly Ser Glu Ala Leu Ala Asn Leu Asp Phe Leu Arg Ser Asn Pro

245 250 255

His Phe Gln Gln Leu Arg Gln Leu Val Gln Gln Gln Pro His Met Leu

260 265 270

Glu Pro Ile Leu Gln Gln Val Ala Ala Gly Asn Pro Gln Ile Ser Gln

275 280 285

Ile Ile Gly Gln Asn Ser Glu Gln Phe Leu Gln Leu Leu Ser Glu Glu

290 295 300

Gly Asp Glu Glu Asp Ala Ala Leu Pro Pro Gly Thr Gln Ala Ile Ser

305 310 315 320

Val Thr Glu Glu Glu Arg Asp Ala Ile Glu Arg Leu Cys Arg Leu Gly

325 330 335

Phe Pro Arg Asp Ser Val Ile Gln Ala Tyr Phe Ala Cys Asp Lys Asn

340 345 350

Glu Glu Leu Ala Ala Asn Phe Leu Phe Asp Gln Pro Asp Asp Asp Glu

355 360 365

Glu

<210> 277

<211> 375

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 277

Met Lys Leu Thr Phe Lys Asp Leu Lys Gln Glu Lys Phe Val Ile Glu

1 5 10 15

Val Glu Pro Ser Glu Thr Val Arg Glu Val Lys Gln Lys Ile Ala Gln

20 25 30

Glu Lys Gly Glu Tyr Glu Ala Glu Arg Met Lys Val Ile Tyr Ser Gly

35 40 45

Lys Ile Leu Gln Asp Asp Lys Thr Val Glu Ser Tyr Asn Ile Gln Glu

50 55 60

Lys Asp Phe Leu Val Cys Leu Pro Ser Lys Gly Pro Lys Pro Ala Ala

65 70 75 80

Ser Ser Ser Ala Ser Gln Ala Pro Ala Thr Pro Ala Pro Arg Ala Pro

85 90 95

Val Ala Thr Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Pro Ala Ser

100 105 110

Ser Thr Pro Ala Val Pro Ala Thr Pro Ser Pro Ala Gly Ala Gln Thr

115 120 125

Gly Pro Ser Phe Gly Asp Pro Ser Ala Leu Thr Met Gly Ser Ala Ala

130 135 140

Glu Gly Ala Val Thr Gln Met Glu Ala Met Gly Phe Ala Arg Ser Asp

145 150 155 160

Ile Asp Arg Ala Met Arg Ala Ala Phe Phe Asn Pro Asp Arg Ala Val

165 170 175

Asp Tyr Leu Leu Asn Gly Ile Pro Ala Asp Val Gln Gln Glu Gln Gln

180 185 190

Gln Arg Gln Gln Glu Gln Gln Ala Asp Arg Ala Ala Glu Gln Ala Pro

195 200 205

Val Pro Ser Ala Glu Asp Ala Ala Ala Ala Ala Ala Leu Gly Gly Asp

210 215 220

Glu Gly Phe Asn Met Phe Glu Ala Ala Ala Gln Ala Gly Asp Gly Arg

225 230 235 240

Gly Gly Gly Ala Arg Ser Gly Gly Ser Glu Ala Leu Ala Asn Leu Asp

245 250 255

Phe Leu Arg Ser Asn Pro His Phe Gln Gln Leu Arg Gln Leu Val Gln

260 265 270

Gln Gln Pro His Met Leu Glu Pro Ile Leu Gln Gln Val Ala Ala Gly

275 280 285

Asn Pro Gln Ile Ser Gln Ile Ile Gly Gln Asn Ser Glu Gln Phe Leu

290 295 300

Gln Leu Leu Ser Glu Glu Gly Asp Glu Glu Asp Ala Ala Leu Pro Pro

305 310 315 320

Gly Thr Gln Ala Ile Ser Val Thr Glu Glu Glu Arg Asp Ala Ile Glu

325 330 335

Arg Leu Cys Arg Leu Gly Phe Pro Arg Asp Ser Val Ile Gln Ala Tyr

340 345 350

Phe Ala Cys Asp Lys Asn Glu Glu Leu Ala Ala Asn Phe Leu Phe Asp

355 360 365

Gln Pro Asp Asp Asp Glu Glu

370 375

<210> 278

<211> 342

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 278

Ala Pro Gly Asp Met Glu Thr Ala Asp Ala Lys Asn Arg Ala Met Arg

1 5 10 15

Ala Ala Gly Phe Ile Val Pro Asp Thr Phe Glu Asp Leu Pro Glu Val

20 25 30

Leu Lys Thr Thr Tyr Thr Gly Leu Val Gln Lys Gly Val Ile Val Pro

35 40 45

Lys Ala Glu Ile Asp Pro Pro Asn Ile Pro Met Asp Tyr Gln Trp Ala

50 55 60

Ser Lys Leu Gly Leu Ile Arg Lys Pro Ala Ala Phe Ile Ser Thr Ile

65 70 75 80

Ser Asp Glu Arg Gly Gln Glu Leu Met Tyr Ala Gly Met Arg Ile Ser

85 90 95

Asp Val Phe Lys Glu Glu Ile Gly Ile Gly Gly Val Ile Ser Leu Leu

100 105 110

Trp Phe Lys Arg Arg Leu Pro Pro Phe Ala Cys Lys Phe Ile Glu Met

115 120 125

Val Leu Gln Leu Thr Ala Asp His Gly Pro Ala Val Ser Gly Ala Met

130 135 140

Asn Thr Ile Ile Thr Ala Arg Ala Gly Lys Asp Leu Ile Ser Ser Leu

145 150 155 160

Ala Ala Gly Leu Leu Thr Ile Gly Asp Arg Phe Gly Gly Ala Leu Asp

165 170 175

Gly Ala Ala Ala Glu Phe Ser Arg Gly Leu Asn Ser Gly Ala Thr Pro

180 185 190

Arg Glu Phe Val Asp Ser Met Arg Lys Ala Asn Arg Leu Ile Pro Gly

195 200 205

Ile Gly His Lys Ile Lys Ser Lys Thr Asn Pro Asp Leu Arg Val Val

210 215 220

Leu Val Val Asp Tyr Val Lys Lys His Phe Pro Ser His Lys Thr Leu

225 230 235 240

Asp Phe Ala Leu Ala Val Glu Asp Val Thr Thr Gln Lys Ser Asn Thr

245 250 255

Leu Ile Leu Asn Val Asp Gly Ala Ile Ala Ala Ser Phe Cys Asp Leu

260 265 270

Leu Ser Gly Cys Gly Ala Phe Thr Glu Asp Glu Ala Ala Asp Tyr Leu

275 280 285

Lys Asn Gly Thr Leu Asn Gly Leu Phe Val Leu Gly Arg Ser Ile Gly

290 295 300

Phe Ile Gly His Tyr Leu Asp Gln Arg Leu Leu Lys Gln Pro Leu Tyr

305 310 315 320

Arg His Pro Ala Asp Asp Ile Phe Ile Asn Met Gln Glu Arg Val Val

325 330 335

Phe Gln Pro Gly Ser Asn

340

<210> 279

<211> 182

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 279

Gly Ala Tyr Lys Glu Gly Lys Phe Thr Ser Glu Ser Ile Gln Lys Ser

1 5 10 15

Lys Leu Arg Phe Gln Asp Ile Leu Val Glu Leu Pro Leu Arg Val His

20 25 30

Asn Ser His Leu Leu Thr Ser Phe Leu His Gln Val Pro Gln Ala Pro

35 40 45

Pro Ala Lys Asn Pro Leu Asp Phe Pro Ser Ser Leu Ala Glu Leu Ser

50 55 60

Arg Asp Ser Asp Val Ser Ser Asn Pro Phe Ala Pro Asn Leu Asp Thr

65 70 75 80

Leu Asp Leu Ser Ile Asp Pro Phe Gln Tyr Trp Gln Arg Ala Leu Gly

85 90 95

Arg Glu Gln Gln Lys Ile Thr Ala Trp Gln Gln Lys Arg Lys Ala Glu

100 105 110

Asn Ala Ala Arg Ala Ala Ser Lys Gln Pro Pro Leu Asp Glu Asn Glu

115 120 125

Trp Gln Lys Leu Phe Lys Leu Pro Thr Glu Pro Ser Arg Leu Glu Ala

130 135 140

Leu Leu Val Gly Arg Gln Val Glu Gln Tyr Ala Arg Gln Val Asp Gly

145 150 155 160

Phe Ser Ala Thr Val Ser Ala Lys Met Phe Gly Val Arg Gly Asn Leu

165 170 175

Leu Pro Asn Glu Ile Glu

180

<210> 280

<211> 171

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 280

Met Ser Ala Pro Thr Pro Ser His Pro Thr Leu Thr Pro Trp Thr Ser

1 5 10 15

Ala Ser Thr Pro Ser Ser Thr Gly Ser Ala Pro Ser Ala Ala Ser Ser

20 25 30

Arg Arg Ser Pro His Gly Asn Arg Ser Ala Arg Leu Arg Met Leu His

35 40 45

Ala Pro Arg Ala Ser Ser Arg Pro Leu Thr Arg Met Ser Gly Arg Ser

50 55 60

Cys Ser Ser Cys Pro Arg Ser Pro Ala Gly Ser Arg Leu Cys Leu Ser

65 70 75 80

Ala Gly Arg Ser Ser Ser Thr Pro Ala Arg Ser Thr Asp Ser Pro Pro

85 90 95

Pro Phe Pro Pro Arg Cys Leu Ala Ser Gly Ala Thr Ser Ser Leu Thr

100 105 110

Arg Ser Ser Arg Gly Arg Ile Leu Arg Arg Arg Asp Arg Arg Leu Cys

115 120 125

Ile Ala Arg Arg Ser Arg Leu Gly Gly Val Glu Tyr Met Arg His Gly

130 135 140

Tyr Lys Lys Lys Asp Asp Val Val Pro Pro Ser Thr Ser Ser Gly Gln

145 150 155 160

Arg Cys Ile Glu Ser Gln Lys Lys Lys Gly Phe

165 170

<210> 281

<211> 217

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 281

Met Arg Arg Ser Ser Lys Phe Lys Asn Val Lys Ile Leu Ser Ser Phe

1 5 10 15

Gly Ile Leu Cys Ser Val Ala Leu Asn Ser Ser Met Ala Glu Pro His

20 25 30

His Pro Phe Phe Cys Ser His Ala Ala Cys Thr Leu Pro His Pro Ala

35 40 45

Glu Asn Ala Ser Leu Cys Ile Asn Ala Gly Pro Val Ser Val Ile Phe

50 55 60

Val Leu Tyr Ser Ile Ser Leu Gly Arg Arg Leu Pro Leu Thr Pro Asn

65 70 75 80

Ile Leu Ala Glu Thr Val Ala Glu Asn Pro Ser Thr Trp Arg Ala Tyr

85 90 95

Cys Ser Thr Cys Leu Pro Thr Ser Arg Ala Ser Ser Leu Leu Gly Ser

100 105 110

Val Gly Ser Leu Asn Ser Phe Cys His Ser Phe Ser Ser Arg Gly Gly

115 120 125

Cys Leu Leu Ala Ala Arg Ala Ala Phe Ser Ala Leu Arg Phe Cys Cys

130 135 140

His Ala Val Ile Phe Cys Cys Ser Arg Pro Arg Ala Arg Cys Gln Tyr

145 150 155 160

Trp Lys Gly Ser Met Leu Arg Ser Arg Val Ser Arg Leu Gly Ala Lys

165 170 175

Gly Leu Glu Leu Thr Ser Glu Ser Arg Glu Ser Ser Ala Arg Asp Glu

180 185 190

Gly Lys Ser Arg Gly Phe Phe Ala Gly Gly Ala Cys Gly Thr Trp Cys

195 200 205

Arg Lys Leu Val Arg Arg Trp Glu Leu

210 215

<210> 282

<211> 150

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 282

Met Tyr Ser Thr Pro Pro Ser Arg Glu Arg Leu Ala Met His Lys Arg

1 5 10 15

Arg Ser Arg Leu Arg Asn Ile Arg Pro Leu Leu Asp Leu Val Arg Glu

20 25 30

Glu Val Ala Pro Asp Ala Lys His Leu Gly Gly Asn Gly Gly Gly Glu

35 40 45

Ser Val Asp Leu Ala Gly Val Leu Leu Asp Leu Pro Ala Asp Lys Gln

50 55 60

Ser Leu Glu Pro Ala Gly Leu Arg Gly Gln Leu Glu Gln Leu Leu Pro

65 70 75 80

Leu Ile Leu Val Lys Gly Arg Leu Leu Ala Arg Gly Ala Cys Ser Ile

85 90 95

Leu Ser Leu Ala Leu Leu Leu Pro Cys Gly Asp Leu Leu Leu Leu Ala

100 105 110

Ala Glu Gly Ala Leu Pro Val Leu Glu Gly Val Asp Ala Glu Val Gln

115 120 125

Gly Val Lys Val Gly Cys Glu Gly Val Gly Ala Asp Ile Gly Val Ala

130 135 140

Arg Lys Leu Cys Lys Gly

145 150

<210> 283

<211> 269

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 283

Met Ser Asn Ser Phe Ile Arg His Leu Pro Ser Thr Leu Arg Ala Phe

1 5 10 15

Arg Ser Ser Thr Ala Phe Ser Leu Thr Arg Ser Phe Ser Ser Thr Met

20 25 30

Ala Ser Asn Gly Thr Ser Thr Asn Gly Val Gln His Asp Ala Arg Lys

35 40 45

Val Phe Phe Phe Asp Ile Asp Asn Cys Leu Tyr Pro Lys Ser Tyr Gln

50 55 60

Ile His Asp Lys Met Ala Val Leu Ile Asp Asn Tyr Phe Gln Asn His

65 70 75 80

Leu Ser Leu Ser Gln Glu Asp Ala Thr Thr Leu His Gln Arg Tyr Tyr

85 90 95

Lys Asp Tyr Gly Leu Ala Ile Glu Gly Leu Val Arg His His Lys Val

100 105 110

Asp Pro Leu Glu Tyr Asn Glu Lys Val Asp Asp Ala Leu Pro Leu Asp

115 120 125

Asp Ile Ile Lys Pro Asp Pro Lys Leu Arg Lys Leu Leu Gln Asp Ile

130 135 140

Asp Thr Asp Lys Val Lys Leu Trp Leu Phe Thr Asn Ala Tyr Val Asn

145 150 155 160

His Ala Lys Arg Val Thr Arg Leu Leu Gly Val Asp Asp Leu Phe Glu

165 170 175

Gly Met Thr Phe Cys Asp Tyr Ala Ala Glu Arg Leu Leu Cys Lys Pro

180 185 190

Thr Thr Glu Met Tyr Asn Lys Ala Met Gln Glu Ala Asn Ala Thr Asp

195 200 205

Ile Asp Gln Cys Tyr Phe Val Asp Asp Ser Ala Leu Asn Ala Ala Ala

210 215 220

Ala Met Lys Tyr Gly Trp Lys Thr Ala His Leu Val Glu Pro Thr Ala

225 230 235 240

Lys Pro Pro Pro Gln Pro Val Ser Gln His Gln Ile Ser Asn Leu Glu

245 250 255

Glu Leu Arg Lys Val Phe Pro Glu Val Phe Lys Thr Ser

260 265

<210> 284

<211> 256

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 284

Met Ala Arg Ile Phe Ile Thr Gly Ser Thr Asp Gly Leu Gly Leu Leu

1 5 10 15

Ser Ala Lys Leu Leu Ser Glu Gln Gly His Ser Val Phe Leu His Ala

20 25 30

Arg Asn Ala Glu Arg Ala Ser Gln Ala Lys Ala Ala Val Pro Lys Ala

35 40 45

Gln Gly Val Ile Ile Gly Asp Leu Ser Asn Val Ser Asp Val Lys Gln

50 55 60

Leu Ala Ala Asp Ala Asn Lys Ala Gly Pro Phe Asp Ala Val Val His

65 70 75 80

Asn Ala Gly Leu Gly Leu Thr Thr Asn Gly Gln Lys Thr Ala Glu Gly

85 90 95

Val Ala Gln Ile Phe Ala Val Asn Ser Met Ala Pro Tyr Ile Leu Thr

100 105 110

Ala Leu Met Asp Lys Pro Lys Arg Leu Leu Tyr Val Ser Ser Gly Leu

115 120 125

His Phe Gly Gly Asp Pro Ser Leu Glu Asp Val Thr Trp Ala Thr Arg

130 135 140

Glu Phe Arg Pro Ser Asp Ala Tyr Asn Asp Thr Lys Met Gln Asn Val

145 150 155 160

Met Leu Ser Lys Ala Val Ala Lys Arg Trp Pro Asp Val Gln Ser Gly

165 170 175

Ser Leu Asp Pro Gly Trp Val Lys Thr Lys Leu Gly Gly Ser Ala Ala

180 185 190

Pro Gly Thr Thr Asp Ala Pro Ala Glu Met Ile Ala Glu Tyr Ala Ala

195 200 205

Gly Lys Ser Cys Ala Gly Asp Gln Thr Gly Ala Tyr Leu Thr Pro Arg

210 215 220

Gly Val Glu Glu Pro His Asp Ala Thr Lys Leu Ala Glu Lys Gln Asp

225 230 235 240

Arg Leu Met Gln Ile Tyr Lys Glu Val Ser Gly Val Ser Phe Pro Gln

245 250 255

<210> 285

<211> 201

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 285

Met Lys Leu Cys Leu Leu Gly Glu Arg Asn Thr Arg Tyr Leu Leu Val

1 5 10 15

Asn Leu His Gln Thr Ile Leu Leu Leu Gly Gln Leu Ser Arg Ile Met

20 25 30

Arg Leu Phe His Ala Thr Arg Ser Gln Val Gly Thr Cys Leu Ile Ala

35 40 45

Cys Ala Arg Phe Ala Gly Ser Val Leu Ser Asn His Leu Cys Trp Ser

50 55 60

Val Gly Gly Ala Arg Arg Gly Arg Pro Ala Glu Leu Ser Leu His Pro

65 70 75 80

Ala Trp Val Lys Arg Ala Ala Leu His Ile Arg Pro Ala Phe Gly Asp

85 90 95

Cys Phe Arg Glu His Asp Val Leu His Leu Cys Ile Val Val Cys Ile

100 105 110

Arg Trp Ser Glu Leu Pro Cys Gly Pro Ser Asp Val Leu Glu Ala Gly

115 120 125

Val Ala Thr Glu Val Gln Ser Gly Ala Asp Val Gln Glu Pro Leu Arg

130 135 140

Leu Val His Glu Ser Gly Gln Asn Val Arg Cys His Ala Val Asn Gly

145 150 155 160

Lys Asn Leu Gly Tyr Ala Leu Ser Ser Leu Leu Ala Ile Gly Gly Glu

165 170 175

Ser Glu Ala Ser Ile Val Asn Asn Gly Val Lys Arg Ser Ser Leu Val

180 185 190

Gly Ile Gly Gly Glu Leu Leu His Val

195 200

<210> 286

<211> 192

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 286

Met Ala Ile Gly Gln Ser Ser Gln Gln Gln Ala Asp Gly Gln Asn Val

1 5 10 15

Val Thr Gln Gly Asn Ser Asp Lys Ala Ala Asn Pro Met Arg Glu Leu

20 25 30

Arg Ile Gln Lys Leu Val Leu Asn Ile Ser Val Gly Glu Ser Gly Asp

35 40 45

Arg Leu Thr Arg Ala Ala Lys Val Leu Glu Gln Leu Ser Gly Gln Thr

50 55 60

Pro Val Tyr Ser Lys Ala Arg Tyr Thr Val Arg Thr Phe Gly Ile Arg

65 70 75 80

Arg Asn Glu Lys Ile Ser Val His Val Thr Val Arg Gly Ala Lys Ala

85 90 95

Glu Glu Ile Leu Glu Arg Gly Leu Lys Val Lys Glu Tyr Glu Leu Arg

100 105 110

Lys Arg Asn Phe Ser Ala Thr Gly Asn Phe Gly Phe Gly Ile Ser Glu

115 120 125

His Ile Asp Leu Gly Ile Lys Tyr Asp Pro Ala Ile Gly Ile Tyr Gly

130 135 140

Met Asp Phe Tyr Val Val Met Ser Arg Pro Gly Glu Arg Val Ala Arg

145 150 155 160

Arg Arg Arg Ala Lys Thr Arg Val Gly Ala Ser His Lys Val Asn Ala

165 170 175

Pro Glu Val Ile Lys Trp Tyr Lys Asn Arg Phe Glu Gly Ile Val Arg

180 185 190

<210> 287

<211> 213

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 287

Gly His Thr Gly Asp Val Leu Ser Val Ser Phe Ser Ala Asp Asn Arg

1 5 10 15

Gln Ile Val Ser Ala Ser Arg Asp Arg Thr Ile Lys Leu Trp Asn Thr

20 25 30

Leu Gly Glu Cys Lys Phe Asn Ile Val Asp Asp Gly His Ser Glu Trp

35 40 45

Val Ser Cys Val Arg Phe Ser Pro Asn Pro Val Ile Pro Val Ile Val

50 55 60

Ser Ala Gly Trp Asp Lys Val Val Lys Val Trp Glu Leu Ser Lys Cys

65 70 75 80

Lys Leu Lys Thr Asn His His Gly His Thr Gly Tyr Ile Asn Thr Leu

85 90 95

Ala Val Ser Pro Asp Gly Ser Leu Ala Ala Ser Gly Gly Lys Asp Gly

100 105 110

Ile Thr Met Leu Trp Asp Leu Asn Asp Gly Lys His Leu Tyr Ser Leu

115 120 125

Glu Ala Gly Asp Ile Val Asn Ser Leu Val Phe Ser Pro Asn Arg Tyr

130 135 140

Trp Leu Cys Ala Ala Thr Ala Ser Ser Ile Lys Ile Phe Asp Leu Glu

145 150 155 160

Ser Lys Ser Ile Val Asp Asp Leu Lys Pro Asp Phe Ser Ala Glu Tyr

165 170 175

Ser Asp Lys Ala Gln Lys Pro Gln Cys Thr Ser Leu Ala Trp Ser Ala

180 185 190

Asp Gly Gln Thr Leu Phe Ala Gly Phe Ser Asp Asn Leu Val Arg Val

195 200 205

Trp Val Val Thr Ala

210

<210> 288

<211> 347

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 288

Met Ala Pro Ser Thr Gln Lys Gln Trp Thr Val Lys Asn Gly Glu Gln

1 5 10 15

Asp Phe Asp Gly Leu Val Tyr Gly Asp Ala Pro Val Pro Thr Ala Gly

20 25 30

Asp Ser Glu Val Val Val Lys Leu His Gly Ala Ser Leu Asn Tyr Arg

35 40 45

Asp Leu Ile Ile Pro Lys Gly Lys Tyr Pro Phe Pro Leu Ser Phe Pro

50 55 60

Val Val Pro Gly Ser Asp Gly Ala Gly Glu Val Val Glu Val Gly Ser

65 70 75 80

Lys Val Lys Gln Phe Lys Lys Gly Asp Lys Val Val Thr Leu Phe Asn

85 90 95

Gln Leu His Gln Tyr Gly Pro Val Asp Ala Ala Ala Ala Ser Ser Gly

100 105 110

Leu Gly Gly Ala Val Asp Gly Thr Leu Arg Gln Tyr Gly Val Phe Asn

115 120 125

Glu Asn Gly Val Val Arg Ala Pro Thr Asn Leu Asn Phe Leu Glu Ser

130 135 140

Ser Thr Leu Thr Cys Ala Gly Leu Thr Ser Trp Asn Ala Leu Tyr Gly

145 150 155 160

Leu Lys Pro Leu Leu Pro Gly Gln Thr Val Leu Val Gln Gly Thr Gly

165 170 175

Gly Val Ser Ile Phe Ala Leu Gln Phe Ala Lys Ala Ala Gly Ala Thr

180 185 190

Val Ile Ala Thr Thr Ser Ser Glu Glu Lys Gly Lys Arg Leu Lys Asp

195 200 205

Leu Gly Ala Asp His Val Ile Asn Tyr Lys Thr Gln Thr Asn Trp Gly

210 215 220

Glu Ile Ala Arg Gly Leu Thr Arg Asp Asn Ile Gly Val Asp His Ile

225 230 235 240

Ile Glu Val Gly Gly Ala Gly Thr Leu Glu Gln Ser Phe Lys Cys Ile

245 250 255

Lys Phe Glu Gly Val Ile Ser Ile Ile Gly Phe Leu Gly Gly Met Asn

260 265 270

Pro Ser Thr Ile Pro Asn Val Leu Gln Thr Leu Ser Asn Ile Cys Thr

275 280 285

Val Arg Gly Val Tyr Val Gly Ser Lys Ala Leu Met Asn Asp Met Ile

290 295 300

Asn Ala Ile Glu Ala Asn Asn Ile His Pro Val Val Asp Gly Thr Val

305 310 315 320

Phe Thr Leu Glu Lys Thr Arg Glu Ala Tyr Glu Tyr Met Trp Ala Gln

325 330 335

Lys His Phe Gly Lys Leu Thr Ile Gln Ile Ala

340 345

<210> 289

<211> 518

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 289

Met Leu Glu Gln Gln Tyr Gln Met Arg Lys Glu Gln Gln Val Gln Phe

1 5 10 15

Thr Pro Met Ala Ser Pro Ser Ser Thr Pro Tyr His Met His Gln Asp

20 25 30

Phe Thr Val Pro Gly Asp Phe Phe Ser Pro Leu Thr Ser Pro Ala Leu

35 40 45

His Ala Gln Asn Gln Pro Gln Ser Arg Gln Gln Phe Thr Ala His Gln

50 55 60

Gln Gly Tyr Tyr Thr Asn Pro Ser Thr Ala Ala Ser Ser Ala Ala Pro

65 70 75 80

Ser Pro Ile Asp Ala Asn Gly Asp Val Glu Met Gly Gly Asp Gly Val

85 90 95

Ala Leu Pro Glu Ser Ala Ser Gln Pro Lys Lys Pro Ser Arg Arg Lys

100 105 110

Pro Ala Thr Pro Arg Thr Phe Ala Met Asn Lys Val Lys Gln Ser Pro

115 120 125

Ile Gln Lys Pro Gln Lys Arg Lys Ser Val Ala Leu Ala His Lys Asp

130 135 140

Ala Asp Ala Val Val Gln Asp Ala Gln Arg Ser Gly His Ile Ala Pro

145 150 155 160

Lys Ser Ala Gly Leu Gln Met Pro Pro Pro Phe Glu Ser Ser Glu Asn

165 170 175

Asp Ser Val Ser Pro Glu Ala Leu Asn Asp Leu Pro Met Gly Pro Pro

180 185 190

Pro Arg Pro Gly Ser Val Ser Gln Ser Pro Ala Ile Ala Pro Gln Asn

195 200 205

Gln Ser Val Ser Gly Pro Ala Ala Thr Pro Lys Ser Leu Leu Ser Met

210 215 220

Lys Gly Ala Gln Asp Met Asn Ala Pro Ala Ser Thr Gly Ile Ser Gly

225 230 235 240

Gln Met Gly Gln Ala Ser Leu Glu Asp Leu Glu Leu Pro Glu Ala Ala

245 250 255

Glu Asn Pro Gly Ser Thr Ala Thr His Ser Gln Val Leu Asn Ser Gln

260 265 270

Glu Pro Thr Pro Arg Leu Met Pro Ser Arg Lys Thr Pro Lys Leu Gly

275 280 285

Pro Leu Ser Thr Pro Ser Ser Gly Lys Pro Thr Ser Ala Ser Asn Ser

290 295 300

Pro Ala His Ala Leu Ser Pro Met Thr Ala Ser Thr Pro Ala Gly Leu

305 310 315 320

Leu Lys Asp Lys Lys Asp Asn Lys Gly Gly Arg Ala Thr Ser Lys Lys

325 330 335

Arg Gly Ser Val Ser Thr Thr Asn Ser Ala Met Val Ser Pro Ala Leu

340 345 350

Arg Pro Lys Val Ser Pro Ser Ile Lys Pro Leu Leu Pro Glu Gly Thr

355 360 365

Ser Leu Asn Ser Pro Thr His Ala Leu Leu Leu Ala Ser Lys Ser Asn

370 375 380

Tyr Gln Asn Leu Leu Glu Gly Asn His Leu Pro Gly Ile Ser Tyr Pro

385 390 395 400

Asp Ser Leu Ser Thr Gly Leu Thr Ser Lys Arg Thr Ser His Lys Val

405 410 415

Ala Glu Gln Gly Arg Arg Asn Arg Ile Asn Asp Ala Leu Lys Glu Met

420 425 430

Gln Ala Leu Ile Pro Ala Ser Ser Gly Ala Arg Ala Glu Glu Leu Met

435 440 445

Thr Ala Asp Ala Gly Asp Asp Asp Ser Gln Glu Thr Lys Glu Lys Asp

450 455 460

Arg Asp Ala Ala Val Lys Ser Asn Ser Ser Lys Ala Ala Thr Val Glu

465 470 475 480

Ser Ala Asn Arg Tyr Ile Arg Val Leu Lys Glu Ser Asp Ala Ala Gln

485 490 495

Lys Asp Ala Ile Ala Arg Pro Asn Ser Pro Gly Ser Arg Ser Leu Asp

500 505 510

Pro Pro Asp Leu Asp Asn

515

<210> 290

<211> 126

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 290

Met Arg Asn Ile Leu Leu Val Leu Ala Ser Ala Ala Leu Ala Val Val

1 5 10 15

Ala Gln Lys Pro Asp Leu Asp Val Lys Gly Thr Phe Gly Asp Ala Asn

20 25 30

Pro Phe Ser Lys Val Val Asn Gly Gln Ser Asn Lys Leu Tyr Leu Thr

35 40 45

Leu Asp Asn His Ser Pro Glu Ser Leu Val Val Lys Ser Ile Ser Gly

50 55 60

Ser Trp Ser Glu Lys Thr Ser Ala Ser Ser Gly Gln Glu Lys Phe Leu

65 70 75 80

Lys Asn Ser Thr Thr Gln Glu Lys Leu Thr Val Pro Ile Pro Pro Lys

85 90 95

Ser Glu Gly Ala Phe Gln Pro Pro Thr Val Leu Thr Tyr Gln Phe Trp

100 105 110

Ser Glu Phe Lys Pro Arg Glu Leu Leu Leu Thr Val Leu Gly

115 120 125

<210> 291

<211> 91

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 291

Met Val Leu Ala Leu Val Gly Gly Ala Gly Tyr Leu Ala Tyr Asn Ile

1 5 10 15

Tyr Phe Pro Pro Ala Arg Lys Pro Arg Arg Ser Ala Asn Thr Ala Pro

20 25 30

Thr Asp Ala Pro Ala Ala Pro Ala Asp Pro Asp Glu Trp Ile Pro Val

35 40 45

His His Lys Arg Ala Lys Lys Thr Ser Gly Gly Gly Ala Thr Ser Gly

50 55 60

Glu Glu Ser Glu Ala Thr Glu Gly Tyr Ala Ser Glu Lys Ser Ala Ser

65 70 75 80

Gly Ala Lys Lys Arg Gly Lys Gly Gly Arg Lys

85 90

<210> 292

<211> 252

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 292

Met Ser Asp Asn Asn Asp Gly Asn His Gly Gly Gly Val Gly Ala Ser

1 5 10 15

Tyr Tyr Tyr Gly Gly Ile Ala Ile Ala Leu Cys Leu Val Ile Val Leu

20 25 30

Thr Leu Val Ser Arg Ile Leu Tyr Arg Arg Arg Val Arg Asn Arg Leu

35 40 45

Leu Arg Ala Asn Arg Gln Glu Arg Ile Thr Leu Arg Asp Arg Gly Glu

50 55 60

Ala Pro Gly Leu Pro Thr Tyr Arg Glu Ser Arg Asn Gln Pro Ser Leu

65 70 75 80

Pro Arg Tyr Thr Ala Glu Ala Asp Tyr Ala Pro Pro Pro Gly Pro Pro

85 90 95

Pro Ser Asn Ser Pro Asp Asn Glu Gly His His Phe His Phe His Phe

100 105 110

Pro Ser Leu His Val Pro Gln Ala Leu His Leu Arg Pro Arg Gln Ala

115 120 125

Asp Asp Pro Ala Asp Gln Ile Pro Thr Val Pro Pro Pro Ser Tyr Glu

130 135 140

Pro Pro Lys Tyr Glu Pro Pro Ser Gly Ala Pro Pro Glu Gln Gln Gln

145 150 155 160

Glu Pro Val Ala Ser Gly Ser Ser Glu His His His Gln Gln Ser Ala

165 170 175

Leu Gly Glu His Thr Ala Ala Ala Thr Ala Ala Ala Thr Thr Pro Ala

180 185 190

Glu His Ser Gly Glu Ser Thr Glu Leu Arg Ser Ala Ser Pro Ser Gln

195 200 205

Pro Gln Ser Gln Ser Gln Pro Gln Ala Pro Ala Gln Pro Gln Glu Gln

210 215 220

Asp Tyr Gly Tyr Asp Asp Ala Asp Phe Ile His Pro Glu Glu Arg Arg

225 230 235 240

Arg Ile Glu Ala Ala Gln Arg Asn Asp Pro Gln Thr

245 250

<210> 293

<211> 161

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 293

Met Ser Arg Ile Gly Asp Pro Thr Asn Asn Pro Ala Thr Gln Gln Leu

1 5 10 15

Tyr Ser Asp Arg Pro Leu His Leu Pro Gly Pro Gly Leu Lys Pro Ser

20 25 30

Arg Gln Leu Thr Ile Ser Ser Ala Val Ala Phe Arg Glu Asp Ser Gly

35 40 45

Gln Thr Arg Phe Asn Leu Ile Ser Ser Asp His Arg Glu Val Leu His

50 55 60

Ile Ser Ile Arg Ala Arg Asp Asn Val Leu Val Leu Asn Thr Lys Ala

65 70 75 80

Pro Asp Gly Asp Trp Gly Lys Glu Glu Arg His Asp Leu Lys Pro Leu

85 90 95

Phe Asp Thr Pro Leu Leu Pro Tyr Ile Thr Val Met Ala Thr Lys Asn

100 105 110

Ser Tyr Ile Leu Ser Val Pro Gly Lys Arg Glu Ile Ile Phe Asn Lys

115 120 125

Arg Lys Gly Phe Met Glu Pro Ala Val Arg Ile Glu Tyr Asp Tyr Asp

130 135 140

Glu Met Ser Ala Phe Ser Asp Pro Cys Tyr Ile Thr Val Pro Ser Ser

145 150 155 160

Ser

<210> 294

<211> 84

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 294

Met Pro Gln Glu Ile Lys Asp Ile Lys Asn Leu Leu Glu Ile Ala Arg

1 5 10 15

Arg Lys Asp Ala Arg Ser Ala Arg Ile Lys Lys Thr Lys Thr Val Gly

20 25 30

Ala Lys Gly Glu Pro Ala Gln Leu Thr Lys Phe Lys Ile Arg Cys Ser

35 40 45

Arg Tyr Leu Tyr Thr Leu Val Val Ser Asp Gly Glu Lys Ala Glu Lys

50 55 60

Leu Lys Gln Ser Leu Pro Pro Thr Leu Asn Val Glu Glu Ile Gly Lys

65 70 75 80

Val Ser Lys Lys

<210> 295

<211> 173

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 295

Met Ser His Thr Phe Tyr Asp Gly Thr Ile Val Val Leu Gln Gly Ile

1 5 10 15

Leu Glu Thr Phe Ser His Ile Leu His Lys Ala Glu Glu Ser Pro Asn

20 25 30

Ser Ser Ala Phe Pro Ala Ala Arg Leu His Glu Asp Met Tyr Pro Leu

35 40 45

Thr Asp Gln Ile Arg Leu Ala Thr Gln Phe Ser Glu Tyr Ile Leu Ala

50 55 60

Lys Val Thr Gly Arg Glu Pro Arg Lys Phe Glu Gly Asn Pro Leu Thr

65 70 75 80

Phe Ala Glu Phe Tyr Glu Arg Ile Asp Thr Met Leu Lys Ser Leu Lys

85 90 95

Glu Ala Asp Lys Asp Val Val Asn Ala Asn Ala Asp Lys Glu Glu Leu

100 105 110

Thr Gln Val Gly Pro Thr Ala Lys Ile Glu Leu Ser Asn Ala Ile Tyr

115 120 125

Ala His Arg Ile Ala Leu Pro Asn Ile Tyr Phe His Leu Asn Ile Ala

130 135 140

Tyr Gly Ile Leu Arg Lys Glu Gly Val Pro Leu Gly Lys Leu Asp Tyr

145 150 155 160

Phe Ala Gly Phe Phe Pro Pro Ser Met Ala Gln Gly Lys

165 170

<210> 296

<211> 162

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 296

Met Ser Arg Ile Gly Asp Phe Ala Asn Asn Asn Gln Ala Thr Gln Gln

1 5 10 15

Leu Phe Ser Asp Arg Pro Met Gln Leu Pro Gly Pro Gly Leu Lys Pro

20 25 30

Ser Arg Gln Leu Thr Val Ser Ser Ala Met Ala Phe Arg Trp Asp Ser

35 40 45

Gly Gln Thr Arg Phe Asn Leu Ile Ser Ser Asp Arg Arg Glu Val Leu

50 55 60

His Ile Ser Ile Arg Ala Lys Asp Asp Val Leu Val Leu Asn Thr Lys

65 70 75 80

Ala Pro Asp Gly Asn Trp Gly Lys Glu Glu Arg His Glu Leu Lys Pro

85 90 95

Leu Phe Asp Thr Pro Met Leu Pro Tyr Ile Thr Val Thr Ala Thr Lys

100 105 110

Thr Ser Tyr Ile Leu Ser Val Pro Gly Asn Gln Glu Ile Ile Phe Asn

115 120 125

Lys Arg Lys Gly Phe Met Glu Pro Ala Val Lys Ile Glu Tyr Asp Tyr

130 135 140

Asp Glu Asn Pro Ala Phe Ser Asp Pro Cys Tyr Val Thr Val Pro His

145 150 155 160

Leu Ser

<210> 297

<211> 313

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 297

Met Ser Glu Gln Leu His Tyr Lys Gly Ser Leu Ala Gly His Gly Asn

1 5 10 15

Trp Val Thr Ala Ile Ala Thr Ser Ala Glu Asn Pro Asp Met Ile Leu

20 25 30

Thr Ala Ser Arg Asp Lys Ser Val Ile Val Trp Gln Leu Thr Arg Asp

35 40 45

Asp Ala Gln Tyr Gly Tyr Pro Lys Arg Ile Leu Lys Gly His Asn His

50 55 60

Phe Val Ser Asp Val Ser Ile Ser Tyr Asp Gly Gln Phe Ala Leu Ser

65 70 75 80

Ser Ser Trp Asp Lys Thr Leu Arg Leu Trp Asp Leu Asn Thr Gly Leu

85 90 95

Thr Thr Arg Arg Phe Val Gly His Glu Ala Asp Val Leu Ser Val Ser

100 105 110

Phe Ser Ala Asp Asn Arg Gln Ile Val Ser Gly Ser Arg Asp Arg Thr

115 120 125

Ile Lys Leu Trp Asn Thr Leu Gly Glu Cys Lys Phe Asp Ile Lys Asp

130 135 140

Glu Gly His Ser Glu Trp Val Ser Cys Val Arg Phe Ser Pro Asn Pro

145 150 155 160

Met Asn Pro Val Ile Val Ser Ala Gly Trp Asp Lys Val Val Lys Val

165 170 175

Trp Glu Leu Ser Asn Cys Lys Leu Lys Thr Asn His Tyr Gly His Thr

180 185 190

Gly Tyr Ile Asn Thr Val Ser Val Ser Pro Asp Gly Ser Leu Ala Ala

195 200 205

Ser Gly Gly Lys Asp Gly Ile Thr Met Leu Trp Asp Leu Asn Glu Gly

210 215 220

Lys His Leu Tyr Ser Leu Glu Ala Gly Asp Ile Val Asn Ala Leu Val

225 230 235 240

Phe Ser Pro Asn Arg Tyr Trp Leu Cys Ala Ala Thr Ala Ser Cys Ile

245 250 255

Lys Ile Phe Asp Leu Glu Ser Lys Ser Ile Val Asp Glu Leu Lys Pro

260 265 270

Asp Phe Val Asp Val Gly Lys Asn Ser Arg Glu Pro Glu Ala Val Ser

275 280 285

Leu Ser Trp Ser Ala Asp Gly Gln Thr Leu Phe Ala Gly Phe Thr Asp

290 295 300

Asn Ala Val Arg Val Trp Thr Val Ala

305 310

<210> 298

<211> 107

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 298

Gly Glu Gly Gly Gly Ile His Gly Thr Thr Phe Asn Ser Ile Met Lys

1 5 10 15

Cys Asp Val Asp Val Arg Lys Asp Leu Tyr Gly Asn Ile Val Met Ser

20 25 30

Gly Gly Thr Thr Met Tyr Pro Gly Ile Ala Asp Arg Met Gln Lys Glu

35 40 45

Ile Thr Ala Leu Ala Pro Ser Ser Met Lys Val Lys Ile Ile Ala Pro

50 55 60

Pro Glu Arg Lys Tyr Ser Val Trp Ile Gly Gly Ser Ile Leu Ala Ser

65 70 75 80

Leu Ser Thr Phe Gln Gln Met Trp Ile Ser Lys Gln Glu Tyr Asp Glu

85 90 95

Ser Gly Pro Ser Ile Val His Arg Lys Cys Phe

100 105

<210> 299

<211> 111

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 299

Met Arg Leu Ser Leu Glu Ala Leu Ala Val Asp Asp Arg Arg Ala Ala

1 5 10 15

Leu Val Val Leu Leu Leu Arg Asp Pro His Leu Leu Glu Gly Gly Gln

20 25 30

Arg Ser Gln Asp Gly Thr Thr Asn Pro His Arg Val Phe Thr Leu Arg

35 40 45

Arg Ser Asn Asp Leu Asp Leu His Arg Arg Arg Ser Lys Ser Gly Asp

50 55 60

Phe Leu Leu His Thr Val Gly Asn Thr Arg Val His Ser Ser Thr Thr

65 70 75 80

Arg His Asp Asn Val Ala Ile Glu Ile Leu Thr Asp Val Asn Ile Thr

85 90 95

Leu His Asp Gly Val Glu Gly Gly Ser Val Asp Thr Thr Thr Phe

100 105 110

<210> 300

<211> 213

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 300

Met Ala Phe Phe Pro His Tyr Thr Thr Asn Leu Ser Pro Leu Leu Tyr

1 5 10 15

Leu Leu Asp Asp Asp Tyr Ala Val Tyr Arg Ser Thr Cys Pro Lys Ser

20 25 30

Asn Tyr His His Lys Gln His His Ser Arg Arg Gln Pro Ser Pro Val

35 40 45

Arg Tyr Phe Ser Pro Asn Phe Asp Met Arg Glu Gly Asn Asp Ser Tyr

50 55 60

Tyr Leu Asp Gly Glu Leu Pro Gly Val Asn Gln Asn Asp Val Asp Ile

65 70 75 80

Glu Phe Ser Asp Pro Gln Thr Leu Val Ile Lys Gly Arg Val Glu Arg

85 90 95

Asn Tyr Asn Asn Leu Asp Gly Met Asn Glu Glu Asn Gln Gln Asp Glu

100 105 110

Glu Gln Phe Ser Glu Thr Leu Ser Ser Lys Ser Tyr Gln Pro Thr Val

115 120 125

Glu Asp Glu Asp Glu Ala Asn His Ser Pro Pro Val Ala Thr Pro Thr

130 135 140

Tyr Ser Glu Lys Ser Val Thr Glu Lys Thr Gln Lys Pro Ala Tyr Lys

145 150 155 160

Tyr Arg Asn Ser Glu Arg Ala Ile Gly Glu Phe His Arg Ala Phe Asn

165 170 175

Leu Pro Thr Arg Val Asp Gln Asp Ala Val Arg Ala Thr Leu Arg Asn

180 185 190

Gly Ile Leu Ser Leu Glu Leu Pro Lys Glu Pro Ala Pro Lys Met Lys

195 200 205

Lys Ile Arg Ile Glu

210

<210> 301

<211> 161

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 301

Met Ser Arg Ile Gly Asp Pro Thr Asn Asn Pro Ala Thr Gln Gln Leu

1 5 10 15

Tyr Ser Asp Arg Pro Leu His Leu Pro Gly Pro Gly Leu Lys Pro Ser

20 25 30

Arg Gln Leu Thr Ile Ser Ser Ala Val Ala Phe Arg Glu Asp Ser Gly

35 40 45

Gln Thr Arg Phe Asn Leu Ile Ser Ser Asp His Arg Glu Val Leu His

50 55 60

Ile Ser Ile Arg Ala Arg Asp Asn Val Leu Val Leu Asn Thr Lys Ala

65 70 75 80

Pro Asp Gly Asp Trp Gly Lys Glu Glu Arg His Asp Leu Lys Pro Leu

85 90 95

Phe Asp Thr Pro Leu Leu Pro Tyr Ile Thr Val Met Ala Thr Lys Asn

100 105 110

Ser Tyr Ile Leu Ser Val Pro Gly Lys Arg Glu Ile Ile Phe Asn Lys

115 120 125

Arg Lys Gly Phe Met Glu Pro Ala Val Arg Ile Glu Tyr Asp Tyr Asp

130 135 140

Glu Met Ser Ala Phe Ser Asp Pro Cys Tyr Ile Thr Val Pro Ser Ser

145 150 155 160

Ser

<210> 302

<211> 178

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 302

Met Lys Ala Tyr Trp Tyr Asp Asn Gln Pro Gly Asp Gln Arg Leu Pro

1 5 10 15

His Asp Ser Gly Arg Pro Val Thr Glu Ser Tyr Leu Glu Ser Ile Gly

20 25 30

Val Phe Tyr Arg His Cys Pro Thr Ile Asp Leu Val Asp Ser Leu Ala

35 40 45

Ala Glu Arg Gly Tyr Lys Asn Arg Asp Glu Val Cys Val Ser Pro Gln

50 55 60

Thr Met Gly Asp Val Tyr Glu Glu Lys Val Lys Thr Phe Phe Ser Glu

65 70 75 80

His Leu His Glu Asp Glu Glu Ile Arg Tyr Ile Arg Asp Gly Glu Gly

85 90 95

Tyr Phe Asp Val Arg Gly Gln Glu Asp Glu Trp Val Arg Ile Arg Leu

100 105 110

Ser Lys Asp Asp Leu Ile Ile Leu Pro Ala Gly Ile Tyr His Arg Phe

115 120 125

Thr Thr Asp Asp Lys Asn Tyr Val Lys Ala Met Arg Leu Phe Gln Glu

130 135 140

Glu Pro Lys Trp Thr Pro Leu Asn Arg Gly Pro Glu Val Asp Val Asn

145 150 155 160

Pro His Arg Lys Thr Tyr Leu Glu Thr Val Pro Ser Pro Ala Val Ala

165 170 175

Ala Asn

<210> 303

<211> 216

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 303

Val Ala Ala Gly Tyr Thr Pro Glu Ala Leu Glu Ile Leu Ser Lys Lys

1 5 10 15

Lys Gly Gly Lys Tyr Leu Val Leu Glu Met Asp Glu Thr Tyr Asn Pro

20 25 30

Pro Ala Glu Glu Thr Arg Thr Leu Tyr Gly Val Gln Leu Thr Gln Ala

35 40 45

Arg Asn Asp Ala Val Ile Ser Pro Gln Lys Thr Phe Asn Thr Ile Ile

50 55 60

Thr Pro Lys Asn Thr Glu Ser Leu Pro Glu Ser Ala Leu Arg Asp Leu

65 70 75 80

Thr Val Ala Thr Leu Ala Leu Lys Tyr Thr Gln Ser Asn Ser Val Cys

85 90 95

Tyr Ala Leu Asn Gly Gln Val Val Gly Leu Gly Ala Gly Gln Gln Ser

100 105 110

Arg Ile His Cys Thr Arg Leu Ala Gly Asp Lys Thr Asp Asn Trp Trp

115 120 125

Met Arg Phe His Glu Arg Val Leu Asn Ile Lys Trp Lys Gln Gly Thr

130 135 140

Lys Arg Ala Asp Lys Ser Asn Ala Ile Asp Leu Leu Cys Ser Gly Gln

145 150 155 160

Thr Pro Arg Asn Asp Ala Glu Lys Val Glu Tyr Glu Arg Val Phe Ala

165 170 175

Glu Val Pro Ala Pro Phe Thr Gln Glu Glu Arg Asp Ala Trp Leu Ser

180 185 190

Gln Leu Thr Asn Val Ala Ile Ser Ser Asp Ala Phe Val Cys Leu Ser

195 200 205

Pro Leu Leu Glu His Ser Lys Phe

210 215

<210> 304

<211> 51

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 304

Phe Pro Phe Ile Asp Asn Val Phe Arg Ala Ala Arg Ser Gly Val Lys

1 5 10 15

Tyr Ile Ala Ala Pro Ser Gly Ser Gln Asn Asp Gly Pro Val Phe Glu

20 25 30

Thr Ala Glu Lys Leu Gly Ile Ser Phe Val Glu Gln Gly Thr Arg Leu

35 40 45

Phe His His

50

<210> 305

<211> 255

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 305

Val Ala Ala Gly Tyr Thr Pro Glu Ala Leu Glu Ile Leu Ser Lys Lys

1 5 10 15

Lys Gly Gly Lys Tyr Leu Val Leu Glu Met Asp Glu Thr Tyr Asn Pro

20 25 30

Pro Ala Glu Glu Thr Arg Thr Leu Tyr Gly Val Gln Leu Thr Gln Ala

35 40 45

Arg Asn Asp Ala Val Ile Ser Pro Gln Lys Thr Phe Asn Thr Ile Ile

50 55 60

Thr Pro Lys Asn Thr Glu Ser Leu Pro Glu Ser Ala Leu Arg Asp Leu

65 70 75 80

Thr Val Ala Thr Leu Ala Leu Lys Tyr Thr Gln Ser Asn Ser Val Cys

85 90 95

Tyr Ala Leu Asn Gly Gln Val Val Gly Leu Gly Ala Gly Gln Gln Ser

100 105 110

Arg Ile His Cys Thr Arg Leu Ala Gly Asp Lys Thr Asp Asn Trp Trp

115 120 125

Met Arg Phe His Glu Arg Val Leu Asn Ile Lys Trp Lys Gln Gly Thr

130 135 140

Lys Arg Ala Asp Lys Ser Asn Ala Ile Asp Leu Leu Cys Ser Gly Gln

145 150 155 160

Thr Pro Arg Asn Asp Ala Glu Lys Val Glu Tyr Glu Arg Val Phe Ala

165 170 175

Glu Val Pro Ala Pro Phe Thr Gln Glu Glu Arg Asp Ala Trp Leu Ser

180 185 190

Gln Leu Thr Asn Val Ala Ile Ser Ser Asp Ala Phe Phe Pro Phe Ile

195 200 205

Asp Asn Val Phe Arg Ala Ala Arg Ser Gly Val Lys Tyr Ile Ala Ala

210 215 220

Pro Ser Gly Ser Gln Asn Asp Gly Pro Val Phe Glu Thr Ala Glu Lys

225 230 235 240

Leu Gly Ile Ser Phe Val Glu Gln Gly Thr Arg Leu Phe His His

245 250 255

<210> 306

<211> 316

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 306

Met Cys Asp Arg Ser Arg Ile Gly Pro Lys Gly Trp Glu Ala Val Val

1 5 10 15

Ser Thr Gln Ala Val Val Ser Thr Gln Ala Val Val Ser Thr Gln Ala

20 25 30

Val Val Ser Thr Gln Ala Val Val Ser Thr Gln Ala Val Val Ser Thr

35 40 45

Gln Ser Ala Gln Pro Gly Ala Ile Ser Ala Pro Val Ala Ala Gly Lys

50 55 60

Asp Val Glu Leu Gln Trp Thr Glu Trp Pro Glu Ser His His Gly Pro

65 70 75 80

Val Ile Thr Tyr Leu Ala Asn Cys Asn Gly Asp Cys Ser Glu Val Asp

85 90 95

Lys Ser Ser Leu Glu Phe Phe Lys Ile Asp Gln Lys Gly Leu Ile Asp

100 105 110

Asp Ser Asn Val Pro Gly Thr Trp Ala Thr Asp Lys Leu Ile Ser Asn

115 120 125

Asn Asn Ser Tyr Thr Val Thr Ile Pro Ser Asp Ile Ala Ala Gly Asn

130 135 140

Tyr Val Leu Arg His Glu Ile Ile Ala Leu His Ser Ala Gly Asn Glu

145 150 155 160

Asp Gly Ala Gln Asn Tyr Pro Gln Cys Leu Asn Leu Lys Val Thr Gly

165 170 175

Gly Gly Asn Ala Ser Pro Ser Gly Thr Leu Gly Thr Lys Leu Tyr Asn

180 185 190

Glu Asp Asp Ser Gly Ile Leu Val Ser Ile Tyr Gln Gln Leu Asp Ser

195 200 205

Tyr Asp Ile Pro Gly Pro Ala Leu Tyr Ser Gly Ala Ser Ser Ser Ser

210 215 220

Asn Ser Gly Ser Ser Ser Ser Val Ala Ser Ala Thr Ala Ser Ala Thr

225 230 235 240

Ser Ala Ala Ala Ser Ser Pro Ser Ser Ser Gln Ala Ser Gly Thr Pro

245 250 255

Ala Ser Gln Val Lys Ala Gln Thr Ala Ser Ser Thr Pro Ser Ala Ser

260 265 270

Ser Gly Ala Thr Ser Gly Ser Leu Ser Asp Tyr Phe Ser Ser Leu Ser

275 280 285

Ala Glu Glu Phe Leu Asn Val Ile Ser Glu Thr Leu Ser Trp Leu Val

290 295 300

Thr Asp Lys Ile His Ala Arg Asp Leu Ser Thr Ala

305 310 315

<210> 307

<211> 138

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 307

Met Lys Ser Leu Leu Cys Thr Pro Leu Ala Thr Arg Met Val Pro Arg

1 5 10 15

Thr Thr Pro Ser Val Ser Thr Ser Arg Leu Leu Val Val Ala Thr Leu

20 25 30

Leu Pro Gln Val Leu Leu Val Pro Ser Ser Thr Thr Arg Thr Thr Arg

35 40 45

Val Ser Leu Ser Val Ser Thr Ser Ser Leu Thr Pro Thr Thr Ser Pro

50 55 60

Ala Leu Leu Cys Thr Leu Ala Leu Pro Arg Pro Pro Thr Leu Val Leu

65 70 75 80

Leu Pro Ala Leu Leu Arg Pro Leu Leu Leu Pro Leu Leu Pro Leu Leu

85 90 95

Pro Leu Pro Arg Pro Leu Arg Leu Pro Val Pro Pro Leu Pro Arg Ser

100 105 110

Arg Leu Arg Pro Leu Ala Leu Leu Leu Ala Leu Arg Pro Val Pro Leu

115 120 125

Pro Ala Val Cys Pro Thr Thr Ser Ala Leu

130 135

<210> 308

<211> 272

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 308

Val Val Ser Thr Gln Ser Ala Gln Pro Gly Ala Ile Ser Ala Pro Val

1 5 10 15

Ala Ala Gly Lys Asp Val Glu Leu Gln Trp Thr Glu Trp Pro Glu Ser

20 25 30

His His Gly Pro Val Ile Thr Tyr Leu Ala Asn Cys Asn Gly Asp Cys

35 40 45

Ser Glu Val Asp Lys Ser Ser Leu Glu Phe Phe Lys Ile Asp Gln Lys

50 55 60

Gly Leu Ile Asp Asp Ser Asn Val Pro Gly Thr Trp Ala Thr Asp Lys

65 70 75 80

Leu Ile Ser Asn Asn Asn Ser Tyr Thr Val Thr Ile Pro Ser Asp Ile

85 90 95

Ala Ala Gly Asn Tyr Val Leu Arg His Glu Ile Ile Ala Leu His Ser

100 105 110

Ala Gly Asn Glu Asp Gly Ala Gln Asn Tyr Pro Gln Cys Leu Asn Leu

115 120 125

Lys Val Thr Gly Gly Gly Asn Ala Ser Pro Ser Gly Thr Leu Gly Thr

130 135 140

Lys Leu Tyr Asn Glu Asp Asp Ser Gly Ile Leu Val Ser Ile Tyr Gln

145 150 155 160

Gln Leu Asp Ser Tyr Asp Ile Pro Gly Pro Ala Leu Tyr Ser Gly Ala

165 170 175

Ser Ser Ser Ser Asn Ser Gly Ser Ser Ser Ser Val Ala Ser Ala Thr

180 185 190

Ala Ser Ala Thr Ser Ala Ala Ala Ser Ser Pro Ser Ser Ser Gln Ala

195 200 205

Ser Gly Thr Pro Ala Ser Gln Val Lys Ala Gln Thr Ala Ser Ser Thr

210 215 220

Pro Ser Ala Ser Ser Gly Ala Thr Ser Gly Ser Leu Ser Asp Tyr Phe

225 230 235 240

Ser Ser Leu Ser Ala Glu Glu Phe Leu Asn Val Ile Ser Glu Thr Leu

245 250 255

Ser Trp Leu Val Thr Asp Lys Ile His Ala Arg Asp Leu Ser Thr Ala

260 265 270

<210> 309

<211> 138

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 309

Met Lys Ser Leu Leu Cys Thr Pro Leu Ala Thr Arg Met Val Pro Arg

1 5 10 15

Thr Thr Pro Ser Val Ser Thr Ser Arg Leu Leu Val Val Ala Thr Leu

20 25 30

Leu Pro Gln Val Leu Leu Val Pro Ser Ser Thr Thr Arg Thr Thr Arg

35 40 45

Val Ser Leu Ser Val Ser Thr Ser Ser Leu Thr Pro Thr Thr Ser Pro

50 55 60

Ala Leu Leu Cys Thr Leu Ala Leu Pro Arg Pro Pro Thr Leu Val Leu

65 70 75 80

Leu Pro Ala Leu Leu Arg Pro Leu Leu Leu Pro Leu Leu Pro Leu Leu

85 90 95

Pro Leu Pro Arg Pro Leu Arg Leu Pro Val Pro Pro Leu Pro Arg Ser

100 105 110

Arg Leu Arg Pro Leu Ala Leu Leu Leu Ala Leu Arg Pro Val Pro Leu

115 120 125

Pro Ala Val Cys Pro Thr Thr Ser Ala Leu

130 135

<210> 310

<211> 305

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 310

Met Leu Ser Met Phe Thr Arg Val Ala Arg Gly Gln Ala Lys Val Phe

1 5 10 15

Thr Arg Asn Ala Ser Thr Ala Ser Ser Lys Pro Thr Asn Gln Ser Ser

20 25 30

Asn Lys Ala Ala Thr Ile Ala Ala Ser Ile Ser Gly Val Thr Ala Ala

35 40 45

Leu Tyr Ala His Gln Tyr Gly Leu Ile Asp Ser Val Phe Ala Ser Gly

50 55 60

Leu Glu Glu Gly Leu His Ala Pro His Phe Pro Trp Ser His Asn Gly

65 70 75 80

Trp Leu Asp Ser Phe Asp His Asn Ser Ile Arg Arg Gly Tyr Gln Val

85 90 95

Tyr Arg Glu Val Cys Ser Ser Cys His Ser Leu Asp Arg Ile Ala Trp

100 105 110

Arg Asn Leu Val Ala Val Ser His Thr Ser Asp Glu Ala Arg Ala Met

115 120 125

Ala Glu Glu Gln Glu Tyr Thr Asp Gly Pro Asn Asp Gln Gly Glu Ser

130 135 140

Phe Gln Arg Pro Gly Lys Leu Ala Asp Tyr Met Pro Ala Pro Tyr Pro

145 150 155 160

Asn Glu Glu Ala Ser Arg Ala Ala Asn Gly Gly Ala Leu Pro Pro Asp

165 170 175

Leu Ser Leu Ile Val Lys Ala Arg His Gly Gly Ala Asp Tyr Ile Met

180 185 190

Ala Leu Leu Thr Gly Tyr Gln Asp Pro Pro Ala Gly Ile Gln Val Gln

195 200 205

Glu Gly Met Asn Phe Asn Pro Tyr Phe Pro Gly Gly Gly Ile Ala Met

210 215 220

Gly Arg Val Leu Phe Asp Gly Leu Val Glu Tyr Asp Asp Gly Thr Pro

225 230 235 240

Ala Thr Thr Thr Gln Met Ala Lys Asp Val Ala Thr Phe Leu Ser Trp

245 250 255

Ala Ser Glu Pro Glu His Asp Asp Arg Lys Lys Met Gly Phe Gln Ala

260 265 270

Val Ile Ile Leu Ser Ala Met Thr Ala Ile Ser Leu Tyr Val Lys Arg

275 280 285

Leu Lys Trp Ser Pro Ile Lys Thr Arg Lys Leu Thr Tyr Asn Pro Pro

290 295 300

Lys

305

<210> 311

<211> 165

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 311

Gly Gly Ser Pro Ala Lys Lys Ser Leu Ile Gly Ala Met Glu Ala Gln

1 5 10 15

Asn Leu Lys Thr Phe Pro Lys Gln Pro Ile Phe Gln Asn Ser Lys Thr

20 25 30

Arg Gly Asn Lys Lys Val Thr Lys Asp Arg Arg Trp Tyr Lys Asp Val

35 40 45

Gly Leu Gly Phe Lys Thr Pro Gln Glu Ala Ile Thr Gly Thr Tyr Ile

50 55 60

Asp Lys Lys Cys Pro Trp Thr Gly Glu Val Ser Ile Arg Gly Arg Ile

65 70 75 80

Leu Ser Gly Lys Val Val Ser Thr Lys Met Thr Arg Thr Ile Val Ile

85 90 95

Arg Arg Glu Tyr Leu His Tyr Val Pro Lys Tyr Asn Arg Tyr Glu Lys

100 105 110

Arg His Lys Asn Leu Pro Val His Ala Ser Pro Ala Phe Arg Ile Glu

115 120 125

Asn Gly Asp Gln Val Val Val Gly Gln Cys Arg Pro Leu Ser Lys Thr

130 135 140

Val Arg Phe Asn Val Leu Arg Val Ile Lys Asn Lys Ala Ala Ala Lys

145 150 155 160

Ala Phe Ala Lys Phe

165

<210> 312

<211> 72

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 312

Met Pro Pro Arg Lys Pro Arg Cys Ser Phe Lys Glu Cys Lys Glu Gln

1 5 10 15

Ala Gln Arg Ile Val Gly Asp Cys Ser Phe Cys Ser Gly His Phe Cys

20 25 30

Ser Lys His Arg Met Leu Glu Ala His Ser Cys Ser Gly Leu Glu Asp

35 40 45

Cys Lys Lys Glu Ser His Ala Arg Asn Ala Asp Lys Leu Asn Ser Glu

50 55 60

Arg Thr Gln Val Ile Lys Gly Val

65 70

<210> 313

<211> 161

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 313

Met Ser Arg Asn Phe Gly Asp Phe Ser Thr Asn Gln Ala Thr Gln Gln

1 5 10 15

Leu Tyr Ser Asp Arg Pro Leu His Leu Pro Gly Asn Gly Leu Lys Pro

20 25 30

Ala Arg Gln Leu Thr Ile Ser Ser Ala Val Ala Phe Arg Trp Asp Ser

35 40 45

Asp Gln Thr Arg Phe Asn Leu Ile Ser Ser Asp Arg Arg Glu Val Leu

50 55 60

His Ile Ser Ile Arg Ala Lys Asp Asn Val Leu Val Leu Asn Thr Lys

65 70 75 80

Ala Pro Asp Gly Asp Trp Gly Arg Glu Glu Arg His Glu Leu Lys Lys

85 90 95

Leu Phe Asp Thr Pro Met Leu Pro Tyr Ile Thr Val Thr Ala Thr Lys

100 105 110

Met Thr Tyr Asn Ile Thr Val Pro Ser Gly Gln Glu Ile Ile Phe Asn

115 120 125

Lys Arg Lys Gly Phe Met Glu Pro Ala Val Lys Ile Glu Tyr Asp Tyr

130 135 140

Asp Glu His Ser Ala Phe Ser Asp Pro Cys Tyr Ile Thr Val Pro Ser

145 150 155 160

Ser

<210> 314

<211> 119

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 314

Leu Leu Ala Ser Asn Ser Arg Ser Asn Glu Leu Ser Ser Pro Pro Ser

1 5 10 15

Thr His Leu His Ile Ser Gln Ser Thr Met Val Ser Lys Ile Leu Phe

20 25 30

Trp Ser Gly Phe Gly Ile Ala Val Arg Leu Trp Gln Leu Gly Ile Glu

35 40 45

Met Arg Pro Ile Leu Ala Lys Gln Gly Leu Trp Ala Tyr Pro Val Phe

50 55 60

Ala Gly Val Gly Gly Ser Phe Gly Tyr Trp Leu Gln Gly Val Glu Asp

65 70 75 80

Arg Gln Leu Lys Ile Leu Ala Gln Arg Arg Glu Ala Ile Leu Asp Lys

85 90 95

Arg Arg Arg Arg Asp Glu Arg Glu Gly Leu Ser Asn Ile Glu Lys Glu

100 105 110

Gly Thr Leu Ala Ala Thr Pro

115

<210> 315

<211> 91

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 315

Leu Asn Trp Ser Cys Asn Phe Ala Asp Cys Trp Pro Arg Thr Pro Asp

1 5 10 15

Arg Thr Asn Ser Pro Leu Leu Arg Gln Arg Thr Phe Thr Tyr Arg Lys

20 25 30

Ala Gln Trp Phe Pro Arg Phe Ser Ser Gly Val Ala Ser Ala Ser Pro

35 40 45

Ser Val Ser Gly Asn Ser Val Ser Lys Cys Val Pro Phe Leu Pro Ser

50 55 60

Arg Val Ser Gly Pro Thr Pro Ser Ser Gln Val Ser Val Glu Ala Ser

65 70 75 80

Val Thr Gly Ser Arg Val Ser Arg Thr Val Ser

85 90

<210> 316

<211> 182

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 316

Met Ile His Ala Gln Gln Cys Asn Cys Arg Ser Phe Ser Glu Gly Ser

1 5 10 15

Glu Asn Lys Gln Gln Pro Thr Gln Gln Ile Met Gly Ser Gln Pro Lys

20 25 30

Tyr Pro Pro Ser Gln Cys Cys Ser Asp Pro His Ala Arg Pro Val Ser

35 40 45

Gly Ala Cys Arg Gly Trp Leu Arg Gly Ala Ala Gln Glu Ser Ser Ala

50 55 60

Asp Gly Pro Arg His Pro Gly Ala Ser Asn Arg Ser Phe His Arg His

65 70 75 80

Leu Arg Arg Arg Gly Arg Pro Arg Asp Pro Ala Trp Gln Glu Trp Asp

85 90 95

Ala Phe Arg Tyr Arg Val Ala Arg Asp Gly Arg Arg Cys Arg Ser His

100 105 110

Ser Arg Arg Glu Ser Trp Lys Pro Leu Cys Phe Ala Ile Cys Glu Gly

115 120 125

Ala Leu Thr Glu Glu Arg Arg Val Arg Ser Ile Gly Ser Ser Arg Pro

130 135 140

Ala Ile Ser Glu Ile Ala Gly Pro Ile Gln Thr Pro Ala Thr Lys Ala

145 150 155 160

Asn Thr Ala Gly Asp Asn Arg Glu Ser Gly Glu Ser Thr Pro Ala Asn

165 170 175

Lys Lys Phe Ser Ala Arg

180

<210> 317

<211> 127

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 317

Met Pro Ser Asn Ala Ile Ala Gly Leu Ser Pro Lys Ala Val Lys Ile

1 5 10 15

Asn Asn Ser Gln Arg Asn Lys Ser Trp Gly Arg Ser Gln Ser Thr Leu

20 25 30

Leu Leu Asn Val Ala Gln Thr Leu Thr Leu Val Pro Ser Pro Ala Leu

35 40 45

Val Glu Asp Gly Phe Ala Ala Leu Arg Lys Asn Leu Gln Leu Thr Val

50 55 60

Leu Asp Thr Leu Glu Pro Val Thr Glu Ala Ser Thr Asp Thr Cys Glu

65 70 75 80

Asp Gly Val Gly Pro Glu Thr Leu Leu Gly Lys Asn Gly Thr His Phe

85 90 95

Asp Thr Glu Leu Pro Glu Thr Asp Gly Asp Ala Glu Ala Thr Pro Glu

100 105 110

Glu Asn Leu Gly Asn His Cys Ala Leu Arg Tyr Val Lys Val Arg

115 120 125

<210> 318

<211> 300

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 318

Met Pro Phe Ile Lys Glu Ala Lys Ser Asn Ser Tyr Phe Ser Arg Tyr

1 5 10 15

Gln Val Lys Tyr Arg Arg Arg Arg Glu Gly Lys Thr Asp Phe Tyr Ala

20 25 30

Arg Lys Arg Leu Val Thr Gln Ala Lys Asn Lys Tyr Asn Ala Pro Lys

35 40 45

Tyr Arg Leu Val Val Arg Phe Thr Asn Lys Asp Ile Ile Cys Gln Ile

50 55 60

Val Ser Ser Lys Leu Gln Gly Asp Val Val Leu Thr His Ala Arg Ala

65 70 75 80

Arg Glu Leu Pro Arg Tyr Gly Ile Lys His Gly Leu Thr Ser Trp Ser

85 90 95

Ser Ala Tyr Ala Val Gly Leu Leu Val Ala Arg Arg Ala Leu Thr Lys

100 105 110

Leu Gly Leu Ala Asp Lys Tyr Glu Gly Asp Val Glu Ala Thr Gly Glu

115 120 125

Tyr Asn Leu Thr Glu Pro Leu Gly Asp Asp Glu Pro Arg Pro Phe Lys

130 135 140

Val Phe Leu Asp Val Gly Leu Lys Arg Thr Ser Thr Gly Ser Arg Val

145 150 155 160

Phe Gly Ala Leu Lys Gly Ala Ser Asp Gly Gly Leu Tyr Ile Pro His

165 170 175

Ser Glu Asn Arg Phe Pro Gly Tyr Asp Ile Glu Ser Lys Glu Leu Asp

180 185 190

Ala Glu Ile Leu Asn Lys Tyr Ile Leu Gly Gly His Ile Ala Glu Tyr

195 200 205

Met Glu Ala Leu Glu Glu Glu Asp Glu Glu Arg Phe Lys Ala Gln Phe

210 215 220

Ser Thr Tyr Leu Glu Asp Gly Ile Gly Ser Glu Asp Ile Glu Glu Ile

225 230 235 240

Phe Ser Gly Ala His Glu Ala Ile Arg Ala Asp Pro Thr Phe Lys Pro

245 250 255

Ser Glu Ala Ala Lys Gly Thr Asp Trp Lys Ser Glu Ser Lys Lys His

260 265 270

Arg Ala Val Arg Leu Thr Lys Gln Gln Arg Glu Asp Ala Ile Gln Gln

275 280 285

Arg Ile Lys Tyr Tyr Gln Gln Ala Gly Asp Leu Glu

290 295 300

<210> 319

<211> 212

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 319

Met Ala Phe Met Asn Leu Pro Trp Pro Thr Glu Cys Leu His Ala Ala

1 5 10 15

Leu Lys Asn Gly Ser Leu Pro Phe Trp Gly Phe Val Ile Tyr Arg Thr

20 25 30

Thr Tyr Thr Ala Gln Ser Asp Ala Ala Trp Pro Gln Ile Ile Glu Leu

35 40 45

Ile Ala Ser Tyr Met Lys Ala Leu Leu Tyr His Glu Tyr Asn Asp Lys

50 55 60

Lys Lys Asp Gly Asp Glu Pro Thr Val Tyr Asp Glu Ile Trp Ala Arg

65 70 75 80

His Gln Leu Thr Ile Met Asp Asp Arg Gln Phe Asn Gly Ala Ser Val

85 90 95

Phe Asp Ile Gln Leu His Phe Glu Lys Trp Val Glu Ala Gln Gly Lys

100 105 110

Arg Asp Glu Ser Thr Met Tyr Arg Met Cys Met Val Ile Asp Asp Glu

115 120 125

Ser Ile Gln Thr Leu Leu Glu Ala Pro Pro Gly Glu Asn Arg Lys Leu

130 135 140

Gly Arg Arg Ile Gly Gly Pro Val Arg Phe Val Lys Val Val Glu Ala

145 150 155 160

Phe Pro Glu Leu Asp Ser Leu Asp Glu Phe Gln Gly Trp Met Lys Cys

165 170 175

Glu Ile Asn Ala Leu Trp Pro Leu Trp Lys Met Met Ser Asp Gly Asp

180 185 190

Glu Met Arg Met Ser Tyr Asp Glu Met Lys Gly Asn Gly Lys Gln Val

195 200 205

Tyr Gly Ala Ile

210

<210> 320

<211> 192

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 320

Met Thr Glu Lys Leu Tyr Thr Glu Gln Val Asn Ala Phe Gly Asn Glu

1 5 10 15

Leu Pro Pro Leu Ser Tyr Lys Asp Leu Asp Lys Leu Pro Leu His Gln

20 25 30

Asn Val Ile Lys Glu Thr Leu Arg Ile His Asn Ser Ile His Thr Leu

35 40 45

Met Arg Lys Val Lys Asn Pro Leu Pro Val Pro Gly Thr Arg Phe Val

50 55 60

Ile Pro Thr Ser His Thr Leu Leu Ala Ser Pro Gly Val Thr Thr Arg

65 70 75 80

Asp Asp Ser His Phe Arg Asn Ala Met Thr Trp Asp Pro His Arg Trp

85 90 95

Glu Thr Arg Ser Glu Val Glu Asp Asp Gly Glu Thr Ile Asp Tyr Gly

100 105 110

Tyr Gly Val Val Ser Lys Gly Thr Lys Ser Pro Tyr Leu Pro Phe Gly

115 120 125

Ala Gly Arg His Arg Cys Ile Gly Glu Lys Phe Ala Tyr Leu Asn Leu

130 135 140

Thr Val Ile Val Ala Thr Leu Val Arg Asn Phe Arg Phe Ser Glu Pro

145 150 155 160

Asp Asp Arg Glu Gly Val Pro Glu Thr Asp Tyr Ser Ser Leu Phe Ser

165 170 175

Arg Pro Met Arg Pro Ala Thr Ala Arg Trp Glu Arg Arg Gly Glu Tyr

180 185 190

<210> 321

<211> 210

<212> PRT

<213> 从环境挑战生态位纯化的宏基因组

<400> 321

Met Ala His Trp Arg Glu Glu Tyr Leu Thr Ala Leu Ala Val Arg Asp

1 5 10 15

Gln Arg Glu Lys Ala Asn Leu Ser Ile Tyr Asp Ala Tyr Thr Arg Leu

20 25 30

Ala Asp Ser Thr Ala Lys Leu Pro Ala Thr Ile Asp Thr Ser Gly Ser

35 40 45

Pro Ser Gly Asp Lys Gly Pro Ser Gly Thr Tyr Glu Ser Glu Lys Thr

50 55 60

Ala Phe Ser Gln Ser Arg Thr Ala Lys Lys Gln Gln Thr Glu Val Glu

65 70 75 80

Pro Ser Val Thr Glu Leu Leu Asn Thr Thr Arg Ala Glu Leu Ala Glu

85 90 95

Ala Gln Arg Ser Arg Ala Glu Leu Arg Asp Arg Leu Glu Arg Ala Thr

100 105 110

Asn Glu Ala Glu Lys Leu Arg Lys Gln Ile Gly Lys Asp Gly Arg Arg

115 120 125

Ile His Gly Leu Glu Asn Glu Val Ala Gln Gln Gln Lys Arg Arg Lys

130 135 140

Asp Val Glu Glu Glu Leu Arg Gly Lys Ala Lys Leu Leu Asn Glu Phe

145 150 155 160

Gln Asp Glu Ile Ala Ala Leu Thr Leu Gln Val Asn Met Ala Glu Arg

165 170 175

Lys Ala Lys Lys Leu Gly Glu Glu Asn Asp Asp Leu Val Asn Arg Trp

180 185 190

Met Lys Arg Met Gly Gln Glu Ala Asp Ala Met Asn Asp Ala Ser Lys

195 200 205

Phe Ser

210

<210> 322

<211> 53

<212> DNA

<213> 人工序列

<220>

<223> poly-A mRNA引物

<220>

<221> misc_feature

<222> (52)..(53)

<220>

<221> misc_feature

<222> (52)..(52)

<223> a,c or g

<220>

<221> misc_feature

<222> (53)..(53)

<223> n是a、c、g或t

<400> 322

attctagagc gatcgcacat gttttttttt tttttttttt tttttttttt tvn 53

<210> 323

<211> 34

<212> DNA

<213> 人工序列

<220>

<223> poly-A mRNA引物

<220>

<221> 核糖核苷酸鸟苷

<222> (32)..(33)

<220>

<221> LNA修饰

<222> (34)..(34)

<400> 323

aagcagtggt atcaacgcag agtggcgcgc cggg 34

<210> 324

<211> 34

<212> DNA

<213> 人工序列

<220>

<223> cDNA末端的低封端快速扩增引物

<220>

<221> 5' 反向二脱氧-T

<222> (1)..(1)

<220>

<221> 核糖核苷酸

<222> (2)..(33)

<400> 324

aagcagtggt atcaacgcag agtggcgcgc cggg 34

<210> 325

<211> 33

<212> DNA

<213> 人工序列

<220>

<223> 用于扩增原核生物cDNA文库的引物

<220>

<221> 5' 反向二脱氧-T

<222> (1)..(1)

<220>

<221> 核糖核苷酸

<222> (2)..(33)

<400> 325

taagcagugg uaucaacgca gaguggcgcg ccg 33

<210> 326

<211> 20

<212> DNA

<213> 人工序列

<220>

<223> CaMV 35S启动子的引物

<400> 326

ccactgacgt aagggatgac 20

<210> 327

<211> 20

<212> DNA

<213> 人工序列

<220>

<223> CBF3启动子的引物

<400> 327

cagcatgctc tcactccaac 20

<210> 328

<211> 20

<212> DNA

<213> 人工序列

<220>

<223> Erd10启动子的引物

<400> 328

cgtgagaatg acacaaccac 20

<210> 329

<211> 18

<212> DNA

<213> 人工序列

<220>

<223> Kin1启动子的引物

<400> 329

ctcgtggcac cacactcc 18

<210> 330

<211> 19

<212> DNA

<213> 人工序列

<220>

<223> NOS/HSP终止子的引物

<400> 330

ggaaattcgc ctcgagatc 19

<210> 331

<211> 1391

<212> DNA

<213> 拟南芥（Arabidopsis thaliana）

<400> 331

ataccggagc tcagagaatc atatgactaa ggacgtggtg gttgaaggaa atgagagaat 60

acatgaagaa gagaaacttc tttgagtgag aaggaagtgc gctggctgga gagaaaagag 120

agaaaagagt ttcgagtgag agagagggcg ttgagattgt gatcaactta atgtaatatg 180

ttcttttatt acattttctt tttgtcatat actcaaacct tttactattt tgtctcataa 240

atctaacaca ccccaccatt tgttaatgca tgatggtaga aaatattaaa tataattaac 300

tacttttatg tgatcaaaat taggtttcag actcgtttcg cgatccgatc tacaattaca 360

actgcatgct tctaattgat ctaaattcta aattttttat acatattaaa aaaacaactt 420

tttgttaaat tctcaatcat catttttgtg attaacaatt ttttataact ctaaaccaat 480

aatatttgat tatttatttt atatgtataa tgatgattga gaattttaat tagcagtcta 540

tttagggttt tcctaaagtt acaatatgtt gttacccttc tagttaaatt ttccaaaata 600

ccatatttca taacttttca aactgtttat taattcaacc gtaaaaagca ctaaaatgtt 660

acatttgatc attcacccaa attaaattca aaagtttttc cgccaaaact acttggtgac 720

ttacgtgctt atatacggac gactattatt atgttctata cttttttata ctttgttgca 780

caaatatcta ctctcccaat tcatattcta gaaggatgtg ctataagaat gggagaaatt 840

acacaagaag agcatcttta aatatcctct cacaatcttt atgtctaata cacgggtgaa 900

caattaacga caatttcttt attcaggaat ataataatga ataacggtta ccctacacct 960

agtacactaa atccttaaca gccacacatt catacgcaaa gagtttataa aactcataaa 1020

ggtataataa taacgagtga ataagtcaaa aaaagtcttc tctggacaca tggcagatct 1080

taatgagtga atccttaaac tactcatttt acaattgctt cgctgtgtat agtttacgtg 1140

gcattaccag agacacaaac tccgtcttcg ccttttcttt tgcctctaaa atatcttccg 1200

ccattataaa acagcatgct ctcactccaa cttttattta tctacaaaca ttaaatccac 1260

ctgaactaga acagaaagag agagaaacta ttatttcagc aaaccatacc aacaaaaaag 1320

acagagatct tttagttacc ttatccagtt tcttgaaaca gagtactctt ctgatcaggc 1380

gcgccgccat a 1391

<210> 332

<211> 1215

<212> DNA

<213> 拟南芥

<400> 332

ataccggagc tcgactaggt ggacaaaata atttgttaat taaataaaaa ttagttcaat 60

atagaaatga aaacgattgc tttgtttggt atgtgtcggt acagtgacca tcctaatgcc 120

taatataaaa gattcgatcg gtatgttaca agttgcgtgt atatgaaaac gtcacatttt 180

attaagtggc acgtcgttgt gatgaatctt tcaaccgaac acgattcata atctataagc 240

aaaatccgaa aatggtgcct tctcaatgcc ccctatctgt tcaatctttt tttttttttt 300

tttttttgta tatctgttca atcttattta aatgtaatga caaattaaat gaagtttacg 360

ttagtaatat aagctgacaa acaacaccac attacataca taaaattaaa ttcttttaag 420

tatttcaata acgtttcttt atcttaaaaa ttaaatttac ttcgagagct tcctacttcg 480

tcaaaaataa aattttactt tgctgcatgt ttttactttc tttttgtaac gtcttaaaaa 540

ggtgattaac gtcaacttaa ttcaccgaaa gtctctgcaa ttgatatttt ctgccgacgt 600

ggcataagaa gtccgattgg cccacatgac cgacatccac gcttaaacca atcaaaaccg 660

gccattcagt tccatctgtg ggctcctgaa acgttctctt gacacgtgtt taccatatat 720

tggcttaatc catccatagt ctttctattt actgacaggt agtatttttc ctatcaatta 780

tttattttca cgtggcatga tggctatggc tagttgaacc tgtgaataac ttggtcatat 840

ctactctcta tttatttaga tgattcattt tcttgaagga cttgcaattt tatcccctta 900

cttttatttc tttgagagat aacctaaaat tctcaaaatg agttggaaac atccctttga 960

agttcctcta caggctttct atgtgcataa gaatctgctt aacattggaa ataatatatg 1020

cattcttctc caattctcct agttggatac atatatgaag tctataaatt acacatattt 1080

cccacaaaaa ttattgtaag agtttatatt tcaacatata gtatgcaaac ttaaatcgtg 1140

agaatgacac aaccactaat tcaaaccact acattatata ttctaatcca ttcaaattca 1200

tggcgcgccg ccata 1215

<210> 333

<211> 960

<212> DNA

<213> 拟南芥

<400> 333

ataccggagc tcggtaactt gaattcaacc atgaactgtt tggattggca aacataaact 60

caaataaaat atctaggtat aattgtggtt catacaagaa ttacttcata ctgttgggcc 120

aaaggacgta tccttccccg cacctccaaa ccatgggctt actactgatc cgacatcaaa 180

accgtgttag ttgcaaccaa cgaatgataa gtcaataaga ttcaacttgt caacaaatat 240

acagcttata tgacatgtct ggctccaaac tgaattttag tagaaagtta ctaattcata 300

aaattaattt atatacaatt tttcaatttt tattttataa attaaagaaa aaaacatgaa 360

aaatacggga ggttcggcaa acacaacatt taacttgcca aacgtatcat ctaactttcc 420

caccttatac aaggaaccat tttttcaata ataaagtttt tttttttttg tcttcgcaaa 480

taagagcacg aaatgtttgc caaacgcata tgcaacaaac ccacgttaca taattctgtt 540

tacagccata gagcaagcta tattgttaaa gacctaaaaa aaactttact ataacatata 600

gaggcttcga gatatttcga aagactcaac ttatatataa ataaactcaa aaagaaaaca 660

cggaggcgag aggatcatac tctcacacag aaagagtcac attattatat cctctaaaaa 720

accaaactaa aacgacacgt gaagtcttga tcagccgata aatagctacc gacataaggc 780

aaaactgatc gtaccatcaa atgtaatcca cgtggtttta gattactcgt ggcaccacac 840

tccctttagc ctataaatat aaaccattaa gcccacatct cttctcatca tcactaacca 900

aaacacactt caaaaacgat tttacaagaa ataaatatct gaaaaaggcg cgccgccata 960

Claims (67)

1.一种用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：

a.从预定来源的采样获得遗传物质；

b.从所述遗传物质构建表达文库；

其中所述方法还包括以下步骤：

c.产生用所述表达文库转化的植物，转化效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；

d.筛选表达所述期望性状的转化植物；和

e.鉴定所述转化植物的表达所述期望性状的所述转基因。

2.根据权利要求1所述的方法，还包括根据从所述转基因获得的遗传信息编辑期望作物植物中的靶基因的步骤。

3.根据权利要求2所述的方法，其中使用任何基因组编辑系统或方法进行所述靶基因的所述编辑，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。

4.根据权利要求1所述的方法，其中所述预定来源包括环境生态位的植物、微生物、真菌或其它生物或其部分。

5.根据权利要求1所述的方法，其中所述筛选步骤包括与对照植物相比对所述转化植物的测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、植物地上部分鲜重、叶数、枝鲜重、主枝长度、花产量、荚或果实产量、缺绿症、叶损伤、植物的状态或性能及其任意组合。

6.根据权利要求5所述的方法，其中所述对照植物是与所述转基因植物相同属但是缺少所述转基因的植物，或者与所述转基因植物相同属、缺少所述转基因并且用赋予所述植物改良性状的已知基因转化的植物。

7.根据权利要求1所述的方法，其中所述步骤(a)还包括通过在富培养基或选择性培养基上生长来富集所述遗传物质的步骤。

8.根据权利要求1所述的方法，其中所述步骤(a)还包括通过在针对所述期望性状的选择性培养基上培养所述遗传物质来增强所述期望性状的表达的步骤。

9.根据权利要求1所述的方法，其中所述步骤(b)包括产生原核cDNA文库或真核cDNA文库或两者的步骤。

10.根据权利要求1所述的方法，其中所述步骤(b)还包括将所述cDNA文库克隆到至少一种双元载体中的步骤。

11.根据权利要求10所述的方法，其中所述双元载体包含组成型启动子或胁迫诱导型启动子。

12.根据权利要求10所述的方法，其中所述双元载体包含细菌选择标志物和植物转化选择标志物。

13.根据权利要求10所述的方法，还包括将所述克隆的双元载体转化到宿主细胞中的步骤。

14.根据权利要求10所述的方法，还包括将所述克隆的双元载体转化到根癌农杆菌(Agrobacterium tumefaciens)中的步骤。

15.根据权利要求14所述的方法，还包括将所述转化的根癌农杆菌引入到以下至少一种中的步骤：整株植物、植物组织和植物细胞。

16.根据权利要求15所述的方法，包括通过向所述植物喷洒包含转化的农杆菌的接种物来引入所述转化的根癌农杆菌的步骤。

17.根据权利要求1所述的方法，其中所述步骤(d)包括在对所述期望性状具有选择性的条件下生长所述转化植物。

18.根据权利要求1所述的方法，还包括以下步骤：

f.从步骤(d)的所述转化植物收集T1种子；

g.确定所述T1种子的种子文库转化效率；

h.在允许筛选和选择表达所述期望性状的转化植物的选择条件下播种步骤(e)的所述T1种子；

i.测试步骤(g)的所述选择的表达所述期望性状的植物中所述转基因的存在；和

j.对步骤(h)的对于所述转基因阳性测试的所述选择的转化植物的所述转基因进行分离和测序。

19.根据权利要求18所述的方法，还包括以下步骤：

k.从(h)的发现对于所述转基因的存在为阳性的所述植物收集T2种子；

l.与用赋予所述期望性状的已知基因转化的对照植物相比，在允许筛选和选择表达所述期望性状的转化植物的选择条件下生长所述T2种子的植物；和

m.任选地，对步骤(j)的所述选择的植物的所述转基因进行分离和测序。

20.根据权利要求18和19中的任一项所述的方法，包括以下步骤：

a.对步骤(i)和/或(l)的所述分离的转基因进行重克隆和测序；

b.将所述重克隆的转基因转化到植物中；

c.筛选步骤(b)的所述转化植物以选择表达所述期望性状的转化植物；

d.从步骤(c)的所述选择的植物中分离所述转基因；和

e.任选地，重复步骤(a)至(d)。

21.根据权利要求4所述的方法，其中所述环境生态位包括生态生态位、种群、生境、基因库、原核培养物、真核培养物及其任意组合。

22.根据权利要求4所述的方法，其中所述环境生态位包括微生物组、微生物群、微生物培养物、植物、酵母、藻类、线虫或任何其它生物或其组合。

23.根据权利要求4所述的方法，其中所述环境生态位包括预定的生物因子、非生物因子及其组合。

24.根据权利要求1所述的方法，其中所述采样包括土壤样品、水样品、有机物样品及其任意组合。

25.根据权利要求1所述的方法，其中所述期望性状选自由以下所组成的组：对至少一种生物胁迫的抗性或耐受性、对至少一种非生物胁迫的抗性或耐受性、提高的产量、提高的生物量、提高的食物品质和价值、提高的谷物产量、除草剂或化学物质抗性或耐受性及其任意组合。

26.根据权利要求25所述的方法，其中所述非生物胁迫选自由以下所组成的组：干旱、盐分、热、冷、肥料吸收、肥料使用效率及其任意组合。

27.根据权利要求25所述的方法，其中所述生物胁迫选自由以下所组成的组：植物疾病、病原体、细菌、病毒、真菌、寄生虫、有益和有害昆虫、杂草和栽培的或天然的植物或其任意组合。

28.根据权利要求1所述的方法，其中所述步骤(a)包括从所述预定环境生态位的所述采样中提取RNA的步骤。

29.根据权利要求28所述的方法，其中所述RNA提取根据标准商业试剂盒或根据任何其它用于从环境采样中提取RNA的方案进行。

30.根据权利要求29所述的方法，其中所述用于从环境采样中提取RNA的方案包括以下步骤：

a.获得土壤样品；

b.将所述土壤样品与提取缓冲液混合，所述提取缓冲液包含500mM pH 8的磷酸盐缓冲液和5％w/v的鲸蜡基三甲基溴化铵(CTAB)以及比率为25:24:1的苯酚(pH 8)/氯仿/IAA；

c.使步骤(b)的所述混合物经受37℃下振摇15分钟或珠打浆机1分钟；

d.在室温下将步骤(c)的所述混合物在2500g下离心约10分钟以获得水相；

e.将所述水相转移到新管中；

f.向步骤(e)的所述管内的所述水相添加等量的补充有20mg/ml的结晶紫溶液的异丙醇，以获得紫色染色溶液；

g.通过倒转步骤(f)的所述管来混合所述溶液，然后将所述管在室温下孵育约30分钟；

h.在室温下将步骤(g)的所述管在2500g下离心约30分钟，以获得紫色染色层；

i.将所述紫色染色层转移到新管中，并将所述管以最大速度离心约5min，以获得沉淀和上清液；

j.用80％v/v冰冷的乙醇洗涤所述沉淀并且再离心5分钟，以获得沉淀和上清液；

k.除去步骤(j)的所述上清液并使所述沉淀干燥；和

l.将所述干燥的沉淀以100μl水比2克步骤(a)的土壤的比例混悬在水中。

31.一种植物，包含通过权利要求1至30中任一项所述的方法鉴定的所述转基因。

32.根据权利要求31所述的植物，其中与缺少所述转基因的相同属的植物相比，所述植物具有至少一种植物改良性状。

33.多核苷酸序列，可通过根据权利要求1至30中任一项所述的方法获得。

34.根据权利要求33所述的多核苷酸，其中所述多核苷酸包含与选自由SEQ ID NO:1-148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

35.多核苷酸序列，与根据权利要求33和34中任一项所述的多核苷酸序列具有至少80％序列相似性。

36.多肽序列，可通过根据权利要求1所述的方法获得。

37.根据权利要求36所述的多肽序列，其中所述多肽包含与选自由SEQ ID NO:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

38.多肽序列，与根据权利要求36和37中任一项所述的多肽序列具有至少60％序列相似性。

39.根据权利要求1所述的方法用于鉴定赋予植物改良性状的基因的用途，所述植物改良性状选自由以下所组成的组：对非生物胁迫的抗性或耐受性、对生物胁迫的抗性或耐受性、提高的产量、提高的生物量、提高的食物品质和价值、提高的谷物产量、除草剂或化学物质抗性或耐受性及其任意组合。

40.根据权利要求39所述的用途，其中所述非生物胁迫选自由以下所组成的组：干旱、盐分、热、冷、肥料利用及其任意组合。

41.根据权利要求39所述的用途，其中所述生物胁迫选自由以下所组成的组：植物疾病、病原体、细菌、病毒、真菌、寄生虫、有益和有害昆虫、杂草和栽培的或天然的植物或其任意组合。

42.一种用于筛选和鉴定植物中的干旱或盐分抗性或耐受性改良性状的方法，所述方法包括以下步骤：

a.获得来源于低水分或高盐分来源样品的遗传物质；

b.从所述遗传物质构建表达文库；

其中所述方法还包括以下步骤：

c.产生用所述表达文库转化的植物，转化效率为至少0.5％至30％，代表至少10²-10¹⁰个转基因；

d.筛选对预定干旱或盐分条件具有抗性或耐受性的转化植物；

e.鉴定步骤(d)的所述干旱或盐分抗性或耐受性转化植物的所述转基因。

43.根据权利要求42所述的方法，还包括根据从所述转基因获得的遗传信息编辑期望作物植物中的靶基因的步骤。

44.根据权利要求43所述的方法，其中使用任何基因组编辑系统或方法进行所述靶基因的所述编辑，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。

45.根据权利要求42所述的方法，其中所述预定来源包括环境生态位的植物、微生物、真菌或其它生物或其部分。

46.根据权利要求42所述的方法，其中所述筛选步骤包括与对照植物相比对所述转化植物的测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、植物地上部分鲜重、叶数、枝鲜重、主枝长度、花产量、荚或果实产量、缺绿症、叶损伤、植物的状态或性能及其任意组合。

47.根据权利要求46所述的方法，其中所述对照植物是与所述转基因植物相同属但是缺少所述转基因的植物，或者与所述转基因植物相同属、缺少所述转基因并且用赋予所述植物改良性状的已知基因转化的植物。

48.根据权利要求42所述的方法，其中所述步骤(b)还包括将所述表达文库克隆到至少一种双元载体中的步骤。

49.根据权利要求42所述的方法，还包括以下步骤：

f.从步骤(c)的所述转化植物收集T1种子；

g.将所述T1种子播种在对转化植物有选择性的水含量为约100％容量的土壤中；

h.在干旱或盐分条件下和/或不灌溉的情况下生长所述T1种子的植物，直到大部分植物死亡，以产生在所述干旱或盐分条件下存活的转化植物；

i.使所述干旱或盐分存活转化植物生长以产生T2种子；

j.筛选步骤(i)的所述干旱或盐分存活转化植物中转基因的存在；

k.对步骤(j)的阳性筛选植物的所述转基因进行分离和测序。

50.根据权利要求49所述的方法，还包括以下步骤：

l.从步骤(j)的每一个所述含转基因的阳性筛选的干旱或盐分存活转化植物收集T2种子；

m.与相同属但缺少所述转基因或者用赋予干旱或盐分耐受性或干旱或盐分抗性的已知基因转化的对照植物相比，在预定干旱或盐分条件下从步骤(l)的每一个所述含转基因的T2种子生长T2植物；

n.与所述对照植物相比，对每一个所述含转基因的T2植物进行干旱耐受性或抗性筛选测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、鲜重、叶数、枝鲜重、主枝长度、花和荚产量、缺绿症和叶损伤、植物的状态或性能及其任意组合；

o.从步骤(n)的所述筛选的干旱或盐分抗性性能T2植物分离所述转基因；

p.任选地，将所述转基因重克隆到双元载体中；

q.任选地，将所述克隆的双元载体转化到植物中，并且在预定干旱或盐分条件下生长所述转化植物；和

r.任选地，重复步骤(l)至(q)。

51.根据权利要求50所述的方法，其中所述生长T2植物的步骤包括以下步骤：

a.将所述T2种子播种在对转化植物有选择性的水含量为约100％容量的土壤中；和

b.当所述土壤中的水含量达到约5-10％时，灌溉所述植物。

52.根据权利要求50所述的方法，其中所述预定干旱或盐分条件选自由以下所组成的组：低水分、高盐分、干燥土壤和热。

53.多核苷酸序列，可通过根据权利要求42所述的方法获得。

54.根据权利要求53所述的多核苷酸，其中所述多核苷酸包含与选自由SEQ ID NO:1至SEQ ID NO:148及其任意组合所组成的组的多核苷酸序列中所示的序列相对应的核苷酸序列。

55.多核苷酸序列，与根据权利要求53和54中任一项所述的多核苷酸序列具有至少80％序列相似性。

56.多肽序列，可通过根据权利要求42所述的方法获得。

57.根据权利要求56所述的多肽序列，其中所述多肽序列包含与选自由SEQ ID NO:149-321及其任意组合所组成的组的多肽序列中所示的序列相对应的氨基酸序列。

58.多肽序列，与根据权利要求56和57中任一项所述的多肽序列具有至少60％序列相似性。

59.一种用于从土壤样品中提取RNA的方法，包括以下步骤：

a.获得土壤样品；

b.将所述土壤样品与提取缓冲液混合，所述提取缓冲液包含500mM pH 8的磷酸盐缓冲液和5％w/v的鲸蜡基三甲基溴化铵(CTAB)以及比率为25:24:1的苯酚(pH 8)/氯仿/IAA；

c.使步骤(b)的所述混合物经受37℃下振摇15分钟或珠打浆机1分钟；

d.在室温下将步骤(c)的所述混合物在2500g下离心约10分钟以获得水相；

e.将所述水相转移到新管中；

f.向步骤(e)的所述管内的所述水相添加等量的补充有20mg/ml的结晶紫溶液的异丙醇，以获得紫色染色溶液；

g.通过倒转步骤(f)的所述管来混合所述溶液，然后将所述管在室温下孵育约30分钟；

h.在室温下将步骤(g)的所述管在2500g下离心约30分钟，以获得紫色染色层；

i.将所述紫色染色层转移到新管中，并将所述管以最大速度离心约5min，以获得沉淀和上清液；

j.用80％v/v冰冷的乙醇洗涤所述沉淀并且再离心5分钟，以获得沉淀和上清液；

k.除去步骤(j)的所述上清液并使所述沉淀干燥；和

l.将所述干燥的沉淀以100μl水比2克步骤(a)的土壤的比例混悬在水中。

60.一种用于筛选和鉴定期望的植物改良性状的方法，所述方法包括以下步骤：

a.获得预定来源的采样；

b.根据权利要求60所述的方法从所述采样提取RNA；

c.从步骤(b)的所述RNA构建表达文库；

其中所述方法还包括以下步骤：

d.产生用所述表达文库转化的植物，效率为至少0.05％至30％，代表至少10²-10¹⁰个转基因；

e.筛选表达所述期望性状的转化植物；和

f.鉴定所述转化植物的表达所述期望性状的所述转基因。

61.根据权利要求60所述的方法，还包括根据从所述转基因获得的遗传信息编辑期望作物植物中的靶基因的步骤。

62.根据权利要求61所述的方法，其中使用任何基因组编辑系统或方法进行所述靶基因的所述编辑，包括使用选自由以下所组成的组的工程化核酸酶的系统：大范围核酸酶、锌指核酸酶(ZFN)、基于转录激活因子样效应子的核酸酶(TALEN)、成簇规则间隔的短回文重复序列(CRISPR)系统及其任意组合。

63.根据权利要求60所述的方法，其中所述预定来源包括环境生态位的植物、微生物、真菌或其它生物或其部分。

64.根据权利要求60所述的方法，其中所述筛选步骤包括与对照植物相比对所述转化植物的测量，所述测量选自由以下所组成的组：膨压测量、植物死亡、叶面积、植物地上部分鲜重、叶数、枝鲜重、主枝长度、花产量、荚或果实产量、缺绿症、叶损伤、植物的状态或性能及其任意组合。

65.根据权利要求64所述的方法，其中所述对照植物是与所述转基因植物相同属但是缺少所述转基因的植物，或者与所述转基因植物相同属、缺少所述转基因并且用赋予所述植物改良性状的已知基因转化的植物。

66.分离的多核苷酸，与选自由SEQ ID NO:1至SEQ ID NO:148及其任意组合所组成的组的核苷酸序列具有至少80％序列相似性。

67.分离的多肽，与选自由SEQ ID NO:149-321及其任意组合所组成的组的氨基酸序列具有至少60％序列相似性。

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