CN86101659A

CN86101659A - Operation method of breeding large combined pearls

Info

Publication number: CN86101659A
Application number: CN198686101659A
Authority: CN
Inventors: 张元培
Original assignee: SPECIAL AQUATIC PRODUCT INST HANSHOU COUNTY HUNAN PROV
Current assignee: SPECIAL AQUATIC PRODUCT INST HANSHOU COUNTY HUNAN PROV
Priority date: 1986-02-03
Filing date: 1986-02-03
Publication date: 1987-01-24

Abstract

Operation method of breeding large combined pearls belongs to the breed of pearl.

The present invention adopts the method for in batches inserting nuclear and cell panel stack to cultivate the above large-scale combined pearl of 30mm.

This operation method of breeding is a kind of new method that fresh water genuine pearl with pearl is cultivated, simple possible not only, and can shorten the culture-cycle, remarkable in economical benefits.

Description

Operation method of breeding large combined pearls

Artificial nucleus insertion and pearl, method so far are to insert a nuclear, transplant cell small pieces simultaneously, and the cell small pieces are around the nucleosis encystation, and the secretory cell on the capsule is secreted nacre toward the nuclear face on, deposit nacre and are formed with nucleus pearl.Therefore shape of pearl and li sign indicating number size are determined by the shape of inserting nuclear and size basically.Because the anatomical structure of shellfish body and the specification size of the nuclear that physiological characteristic makes insertion are restricted, thereby the fresh water genuine pearl with pearl diameter reaches 8mm and promptly is considered as large pearl.According to " National Geographic " Aug.1985 report, the diameter that Japanese pearl is cultured the fresh water genuine pearl with pearl of expert Syoichi Kitao cultivation is 17mm, and his target is to cultivate the fresh water genuine pearl with pearl of 20mm.Japan is important in the world pearl culture state, and enlarging nacre is one of their present research direction, on relevant documents and materials, promotes the nutritious method of method of pearl growth, magnetic field breeding method, ray facture or the like.Propose to insert nuclear among the j60110-224 and in the shellfish body, add vitamin, mineral matter, protein, fat, phosphide, monose or nutriments such as polysaccharide, alphalise starch simultaneously, can make the pearl diameter reach 3-12mm; What j5 7125-625 taked is to insert the nuclear back to use the solution-treated shellfish body that contains archusia, protease, inosinicacid or ribonucleic acid to increase the thickness of nacre; J8 4032-088 uses by permanent magnetic and thermoplastic resin to make magnetic basket breed shellfish body; J5 8121-733 proposes the breeze with radium powder and so on, sticks on the nuclear with animal glue, inserts in the shellfish body, can shorten the pearl culture time and produce huge pearl.Though above-mentioned various methodologies can be cultivated bigger pearl,, insert the nuclear specification and be difficult to surpass 15mm, thereby can't cultivate the above large-scale fresh water genuine pearl with pearl of 20mm owing to be subjected to the restriction of naiad body anatomical structure.

The present invention proposes to insert nuclear and cell panel stack method in batches can cultivate the above large-scale combined pearl of 30mm.

The feature of operation method of breeding of the present invention is as follows:

Insert the nuclear position at the latter half of marginal membrane of the body of naiad, cultured pearl shape according to design, insert the nuclear zone at deep layer, middle level and three of shallow-layers respectively, once or in batches insert difform nuclear, and on every nuclear, transplant cell small pieces simultaneously, the cell small pieces are piled up at the position, space between nuclear and the nuclear again; The time interval of in batches inserting nuclear was generally 10-20 days, just can carry out after promptly treating last time to insert the wound healing of examining the position; Inserting the nuclear dividing region is benchmark with the overcoat trace, and deep layer region is embedded in about the interior 1cm of overcoat trace, is the middle level district near the overcoat trace, is the shallow-layer district beyond the overcoat trace, and each nuclear of inserting deep layer region when examining earlier of inserting is inserted the nuclear in middle level district again, inserts the nuclear in shallow-layer district at last; When in a zone, inserting several nuclear simultaneously, can insert nuclear continuously, also can be interrupted and insert nuclear; That inserts endorses to be shell matter pearl nucleus, also can be the pearl that cultivates; Can be white pearl nuclear, also can be colored pearl nuclear; Find during the breed that the nuclear that inserts comes off, in time interpolation is examined and the transplanted cells small pieces, should be adjacent one of junction after-culture between the nuclear simultaneously at the nuclear of interpolation or pile up several piece cell small pieces, closely adopt the pearl phase and find that nuclear comes off, should be according to former design, one of position interpolation and nuclear size, the similar pearl of shape of coming off of coming off, and the transplanted cells small pieces, transplanted cells small pieces too between the pearl of interpolation and the adjacent nuclear.The combining form that the large-scale combined pearl inserts nuclear has following several: the combination (3) of (2) pearl nucleus of the combination between the pearl nucleus of (1) difformity and color and pearl is the combination of pearl all.

The transplanted cells small pieces should select for use 2-3 to make the sexual gland epithelial cell of the latter half of marginal membrane shell-side of the body face of immature shellfish of the same race still, the cell small pieces of preparation exsomatize wait to plant during, instil the cell maintenance liquid for mantle pieces to keep the osmotic pressure balance of cells in-vitro small pieces, stablize the pH value, promote the hyperplasia of pearl sac, keep moistening grade to exsomatize the time-to-live, improve transplanting success to prolong the cell small pieces.The component of cell maintenance liquid for mantle pieces is as follows:

K ₂HPO ₄0.003-0.005g

Cromoci 2ml

（0.1%）

Aureomycin 5ml

（0.1%）

Isotonic water 993ml

(filtration)

When operation freshwater mussel (shellfish) finishes at each slotting nuclear, inject energy mistura (ATP, the compound formulation of coacetylase, the insulin) 1ml of 1% concentration immediately at abdominal foot ditch place.After not culturing one month again, the calcium gluconae of every operation freshwater mussel injection 0.5-1.0%.

The operation freshwater mussel will be cultured in " the best water layer of culturing ", and the physics, chemistry and the biological character that promptly refer to culture the waters are all located the optimum condition that freshwater mussel grows.

Adopt said method, short then one winter an of summer, long then two winters of two summers can be cultivated the large-scale combined pearl more than the 30mm of different shape, various colors.

Embodiment:

1,2 operation method of breeding that " ten " cabinet frame shape pearls are described in detail in detail in conjunction with the accompanying drawings:

Insert the nuclear zone in deep layer (Fig. 1 (2)) earlier and insert nuclear 1, (Fig. 1 (3)) are inserted nuclear 2, nuclear 3, nuclear 4 and are examined 5 in the middle level district again, (Fig. 1 (4)) insert nuclear 7 in the shallow-layer district at last, on every nuclear, transplant cell small pieces immediately respectively after inserting nuclear, after the freshwater mussel of waiting to perform the operation inserts the wound healing at nuclear place, the wound passage inserts nuclear 6, nuclear 8, nuclear 9 and examines 10 again, equally respectively transplanting cell small pieces is close on the nuclear, cell small pieces are respectively transplanted in junction between two nuclear simultaneously, guarantee that all nuclear disjunctors are combined as an integral body.

As finding that during culturing nuclear 4 and nuclear 5 come off, then should be in time at this position interpolation nuclear, and must on the nuclear 4 of interpolation, nuclear 5, transplant cell small pieces again, simultaneously also should be between nuclear 8 and nuclear 4, between nuclear 4 and the nuclear 9, between nuclear 9 and the nuclear 5, examine 5 and examine junction difference interpolation cell small pieces between 10, more roomy as the linkage interface between fruit stone 9 and the nuclear 5, then should pile up 2 to polylith cell small pieces.

Adopt the pearl phase and find that nuclear 6 comes off if close on, then should one of 6 position interpolation of nuclear with examine 6 sizes, the similar pearl of shape, and transplanted cells small pieces, simultaneously also should be between the pearl of nuclear 3 and interpolation, and the pearl of interpolation and the junction of nuclear between 2 transplanted cells small pieces respectively, thereby make the large-scale combined pearl all sites of disjunctor all have the nacre of standard thickness.

Description of drawings:

Fig. 1 shellfish (freshwater mussel) body is inserted the anatomical structure in nuclear zone

1. overcoat trace 4. shallow-layers are inserted the nuclear district

2. deep layer is inserted nuclear district 5. anterior adductor muscles

3. nuclear district 6. posterior adductor muscles are inserted in the middle level

Fig. 2 " ten " cabinet frame shape pearl inserts nuclear and executes the art schematic diagram

1-10 is for inserting the sequence number of nuclear

Claims

1, a kind of operation method of breeding large combined pearls of cultivating fresh water genuine pearl with pearl, insert a chitin pearl nuclear at the position of growing cultured pearls of shellfish body, transplanting cell small pieces simultaneously is close on the nuclear, feature of the present invention is to insert the nuclear position at the latter half of marginal membrane of the body of naiad, pearl shaped according to what design, respectively in deep layer, the middle level, three of shallow-layers are inserted the nuclear zone, once or in batches insert difform nuclear, and while cell small pieces of transplanting on every nuclear, the cell small pieces are piled up at position, space between nuclear and the nuclear again, the pearl nucleus and the cell small pieces that insert are combined as an integral body gradually, thereby cultivate the large-scale combined pearl.

2, operation method of breeding large combined pearls as claimed in claim 1 is characterized in that being generally 10-20 days the blanking time of in batches inserting nuclear, treats that promptly last time inserting the wound healing of examining the position carries out later on.

3, operation method of breeding large combined pearls as claimed in claim 1 is characterized in that each order of inserting nuclear is to insert the nuclear of deep layer region earlier, inserts the nuclear in middle level district again, inserts the nuclear in shallow-layer district at last.

4, as claim 1 and 3 described operation method of breeding large combined pearls, when it is characterized in that in a zone, inserting several nuclears simultaneously, can insert nuclear continuously, also can be interrupted and insert nuclear.

5, operation method of breeding large combined pearls as claimed in claim 1, the nuclear that it is characterized in that once or insert in batches can be difform shell matter pearl nucleus as required, also can be the pearl that cultivates; Can be white pearl nuclear, also can be colored pearl nuclear.

6, operation method of breeding large combined pearls as claimed in claim 1 is characterized in that finding during the pearl culture that the nuclear that inserts comes off, and timely interpolation then, and according to the distance of adopting the pearl phase can the interpolation pearl nucleus, but also interpolation pearl.