CN220079011U - Multichannel quick positioning bracket - Google Patents
Multichannel quick positioning bracket Download PDFInfo
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- CN220079011U CN220079011U CN202222043882.2U CN202222043882U CN220079011U CN 220079011 U CN220079011 U CN 220079011U CN 202222043882 U CN202222043882 U CN 202222043882U CN 220079011 U CN220079011 U CN 220079011U
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- 230000007704 transition Effects 0.000 claims description 9
- 238000010276 construction Methods 0.000 claims 1
- 238000002347 injection Methods 0.000 claims 1
- 239000007924 injection Substances 0.000 claims 1
- 238000004113 cell culture Methods 0.000 abstract description 10
- 238000002474 experimental method Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 8
- 239000006285 cell suspension Substances 0.000 description 4
- 238000001746 injection moulding Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000009471 action Effects 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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Abstract
The utility model relates to the field of experimental equipment, and discloses a multichannel rapid positioning bracket which comprises a positioning plate and supporting plates fixed at two ends of the positioning plate, wherein a plurality of positioning pieces are arranged on the positioning plate, each positioning piece comprises a positioning through hole, a first positioning shell and a second positioning shell, the first positioning shell is fixed on the upper surface of the positioning plate, the second positioning shell is fixed on the lower surface of the positioning plate, the positioning through hole penetrates through the first positioning shell, the positioning plate and the second positioning shell from top to bottom, and the positioning bracket can finish rapid positioning alignment of a pipette gun head and the center of a hole, so that the efficiency and the quality of a cell culture experiment are improved.
Description
Technical Field
The utility model relates to the field of experimental equipment, in particular to a multichannel rapid positioning bracket.
Background
Cell culture refers to a technique in which collected in-vivo tissues are placed under sterile, certain nutritional conditions, appropriate temperature and PH to allow them to grow, reproduce, and maintain their structure and function. The cell culture can directly observe the morphological structure, life activity and change of living cells, and has important roles in cytology, genetics, virology and immunology.
The 96-well plate is a common cell culture device in a laboratory, when cell culture is carried out, cell suspension is generally required to be dripped into the 96-well plate through a liquid-transferring gun, in order to be more beneficial to cell culture and observation, the gun head of the liquid-transferring gun is generally opposite to the center of a hole of the 96-well plate, so that the dripped culture solution presents a small dome shape (as shown in figure 1), but the operation of using the gun head of the liquid-transferring gun opposite to the center of the hole has higher requirement on the operation level of a worker, and the gun mouth of the gun head is easy to contact with the side wall of the hole when the common operator operates, thereby influencing the cell culture quality and being unfavorable for the development of cell culture experiments.
Disclosure of Invention
Therefore, it is necessary to provide a multi-channel rapid positioning support to solve the problem that the muzzle of the pipette gun head cannot be right against the center of the hole when using a 96-well plate for cell culture.
In order to achieve the above purpose, the utility model provides a multichannel quick positioning bracket, which comprises a positioning plate and supporting plates fixed at two ends of the positioning plate, wherein a plurality of positioning pieces are arranged on the positioning plate along the axial direction of the positioning plate, each positioning piece comprises a positioning through hole, a first positioning shell and a second positioning shell, the first positioning shell is fixed on the upper surface of the positioning plate, the second positioning shell is fixed on the lower surface of the positioning plate, and the positioning through holes penetrate through the first positioning shell, the positioning plate and the second positioning shell from top to bottom.
Further, the inner side face of the positioning through hole is provided with a positioning boss. The positioning boss is in positioning and blocking connection with the outer side face of the gun head, so that the gun head can be positioned and enter depth conveniently.
Further, the positioning through hole comprises a first positioning cavity, a second positioning cavity and a third positioning cavity which are connected from top to bottom, the first positioning cavity is in a conical shape with a big top and a small bottom, a conical transition table is arranged at the joint of the first positioning cavity and the second positioning cavity, the positioning boss is positioned in the second positioning cavity, and the third positioning cavity is in a conical shape with a big top and a big bottom.
The conical shape of the first positioning cavity facilitates the alignment and introduction of the gun head of the pipetting gun, and the conical transition table facilitates the introduction of the gun head into the second positioning cavity.
Further, the locating piece, the locating plate and the supporting plate are of an integral injection molding structure. The integral injection molding structure is simple and convenient to manufacture.
Further, a connecting plate is arranged between two adjacent second positioning shells. The connecting plate is used for strengthening the strength of the side face of the second positioning shell, and the durability of the device is improved.
Further, the lateral surface of second positioning shell is equipped with the connection boss, connect boss and connect second positioning shell and locating plate lower surface. The strength of the joint of the second positioning shell and the positioning plate is enhanced by the connecting boss, and the durability of the device is improved.
Further, the upper surface of the first positioning shell and the upper surface of the positioning plate are in arc transition connection.
Further, the plurality of positioning pieces are arranged in a single row on the positioning plate.
Further, the plurality of positioning members are arranged in an array on the positioning plate.
The technical scheme has the following beneficial effects:
the positioning through hole allows the gun head of the liquid-transferring gun to pass through, the second positioning shell corresponds to the pipe orifice of the 96-hole plate or the 8-connection pipe for cell culture in advance, the center of the positioning through hole corresponds to the center of the hole, the gun head can directly face the center of the hole after passing through the positioning through hole, the gun head is prevented from being contacted with the side edge of the hole, and the positioning support can be positioned with a plurality of holes at the same time by arranging a plurality of positioning pieces, so that the operation rate of the liquid-transferring gun is improved.
Drawings
FIG. 1 is a diagram showing the structure of a pipette tip of a pipette just facing the center of a 96-well plate well to which a cell suspension is added.
Fig. 2 is a block diagram of a multichannel rapid positioning bracket according to an embodiment.
Fig. 3 is a cross-sectional structural view of a multichannel rapid positioning bracket according to an embodiment.
Fig. 4 is an enlarged view of the area a of fig. 3.
Fig. 5 is a block diagram of a multichannel rapid positioning bracket according to an embodiment. Reference numerals illustrate:
1. a positioning plate; 2. a support plate; 3. a positioning piece; 4. positioning the through hole; 41. a first positioning cavity; 42. a second positioning cavity; 43. a third positioning cavity; 44. a conical transition stage; 5. a first positioning shell; 6. a second positioning shell; 7. positioning the boss; 8. a connecting plate; 9. a connecting boss; 10. a hole; 20. gun head.
Detailed Description
In order to describe the technical content, constructional features, achieved objects and effects of the technical solution in detail, the following description is made in connection with the specific embodiments in conjunction with the accompanying drawings. Referring to fig. 2-4, the present embodiment provides a multi-channel quick positioning bracket, which includes a positioning plate 1 and a supporting plate 2 fixed at two ends of the positioning plate 1, wherein a plurality of positioning members 3 are arranged on the positioning plate 1 along the axial direction of the positioning plate 1, the positioning members 3 include a positioning through hole 4, a first positioning shell 5 and a second positioning shell 6, the first positioning shell 5 is fixed on the upper surface of the positioning plate 1, the second positioning shell 6 is fixed on the lower surface of the positioning plate 1, and the positioning through hole 4 penetrates through the first positioning shell 5, the positioning plate 1 and the second positioning shell 6 from top to bottom.
In this embodiment, the positioning plate 1 is provided with 8 positioning members 3, which can be matched with a conventional 8-connection tube in use, and can also be applied to a single row corresponding to a 96-well plate.
The inner side surface of the positioning through hole 4 is provided with a positioning boss 7. The positioning boss 7 is positioned and blocked with the outer side surface of the gun head, so that the gun head can be positioned and enter depth conveniently.
Specifically, the positioning through hole 4 comprises a first positioning cavity 41, a second positioning cavity 42 and a third positioning cavity 43 which are connected from top to bottom, the first positioning cavity 41 is in a conical shape with a big top and a small bottom, a conical transition table 44 is arranged at the joint of the first positioning cavity 41 and the second positioning cavity 42, the positioning boss 7 is positioned in the second positioning cavity 42, and the third positioning cavity 43 is in a conical shape with a big top and a big bottom. The taper of the first positioning chamber 41 facilitates the alignment of the pipette tip and the tapered transition stage 44 facilitates the introduction of the tip into the second positioning chamber 42.
In this embodiment, the positioning member 3, the positioning plate 1 and the support plate 2 are an integral injection molding structure. The integral injection molding structure is simple and convenient to manufacture.
In another embodiment, the positioning member 3 may be provided as a detachable device for easy replacement.
A connecting plate 8 is arranged between two adjacent second positioning shells 6. The connecting plate 8 is used for strengthening the strength of the side face of the second positioning shell 6, and improves the durability of the device.
The lateral surface of second positioning shell 6 is equipped with connection boss 9, and connection boss 9 connects second positioning shell 6 and locating plate 1 lower surface. The connecting boss 9 strengthens the strength of the joint of the second positioning shell 6 and the positioning plate 1, and improves the durability of the device. The upper surface of the first positioning shell 5 and the upper surface of the positioning plate 1 are in arc transition connection. The strength of the connection between the first positioning shell 5 and the positioning plate 1 is improved.
When the embodiment is used, the following steps are adopted:
the positioning plate 1 is erected above a 96-well plate through the supporting plate 2, the second positioning shell 6 of the position positioning piece 3 is adjusted to be aligned with holes on the 96-well plate, the pipette absorbs cell suspension, the pipette penetrates through the positioning through hole 4 from the first positioning cavity 41 and penetrates a certain distance, the positioning boss 7 blocks the outer side face of the gun head, the pipette reaches a specified depth, at the moment, the gun muzzle of the gun head is right opposite to the center of the holes, and the cell suspension is released.
As shown in fig. 5, in another embodiment, 96 positioning holes 3 arranged in an array are provided on the positioning plate 1, and in use, the positioning pieces 3 are in one-to-one correspondence with the holes on the 96 well plate. It is noted that relational terms such as first and second, and the like are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or terminal that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or terminal. Without further limitation, an element defined by the statement "comprising … …" or "comprising … …" does not exclude the presence of additional elements in a process, method, article or terminal device comprising the element. Further, herein, "greater than," "less than," "exceeding," and the like are understood to not include the present number; "above", "below", "within" and the like are understood to include this number.
While the embodiments have been described above, other variations and modifications will occur to those skilled in the art once the basic inventive concepts are known, and it is therefore intended that the foregoing description and drawings illustrate only embodiments of the utility model and not limit the scope of the utility model, and it is therefore intended that the utility model not be limited to the specific embodiments described, but that the utility model may be practiced with their equivalent structures or with their equivalent processes or with their use directly or indirectly in other related fields.
Claims (9)
1. The multichannel quick positioning support is characterized by comprising a positioning plate and supporting plates fixed at two ends of the positioning plate, wherein a plurality of positioning pieces are arranged on the positioning plate, each positioning piece comprises a positioning through hole, a first positioning shell and a second positioning shell, the first positioning shell is fixed on the upper surface of the positioning plate, the second positioning shell is fixed on the lower surface of the positioning plate, and the positioning through holes penetrate through the first positioning shell, the positioning plate and the second positioning shell from top to bottom.
2. The multi-channel quick positioning bracket of claim 1, wherein the inner side of the positioning through hole is provided with a positioning boss.
3. The multi-channel rapid positioning bracket according to claim 2, wherein the positioning through hole comprises a first positioning cavity, a second positioning cavity and a third positioning cavity which are connected from top to bottom, the first positioning cavity is conical with a large top and a small bottom, a conical transition table is arranged at the joint of the first positioning cavity and the second positioning cavity, the positioning boss is positioned in the second positioning cavity, and the third positioning cavity is conical with a large top and a large bottom.
4. A multi-channel quick positioning bracket as defined in claim 3, wherein the positioning member, positioning plate and support plate are of unitary injection molded construction.
5. The multi-channel quick positioning bracket of claim 4, wherein a connecting plate is provided between two adjacent second positioning shells.
6. The multi-channel quick positioning bracket of claim 1, wherein the outer side of the second positioning shell is provided with a connecting boss, and the connecting boss connects the second positioning shell and the lower surface of the positioning plate.
7. The multi-channel quick positioning bracket of claim 1, wherein the upper surface of the first positioning shell and the upper surface of the positioning plate are in arc transition connection.
8. The multi-channel quick positioning bracket of any one of claims 1-7 wherein the plurality of positioning members are arranged in a single row on the positioning plate.
9. The multi-channel quick positioning bracket of any one of claims 1-7 wherein a plurality of positioning members are arranged in an array on the positioning plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222043882.2U CN220079011U (en) | 2022-08-04 | 2022-08-04 | Multichannel quick positioning bracket |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202222043882.2U CN220079011U (en) | 2022-08-04 | 2022-08-04 | Multichannel quick positioning bracket |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220079011U true CN220079011U (en) | 2023-11-24 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202222043882.2U Active CN220079011U (en) | 2022-08-04 | 2022-08-04 | Multichannel quick positioning bracket |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220079011U (en) |
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2022
- 2022-08-04 CN CN202222043882.2U patent/CN220079011U/en active Active
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