CN218620767U - Magnetic frame for recovering and purifying PCR (polymerase chain reaction) products and recovering and purifying device thereof - Google Patents

Abstract

The application discloses a magnetic rack for PCR product recovery and purification, which comprises an installation panel, a magnetic assembly and an installation bottom plate. The installation panel is located the mounting plate top, and both butt each other. The mounting panel is provided with a bearing surface, and the bearing surface is provided with a mounting hole array which is regularly distributed; the magnetic component comprises a magnetic element and an outer sleeve surrounding the outer side of the magnetic element, each magnetic component is assembled in a single mounting hole of the mounting hole array, and the outer sleeve is embedded between the magnetic element and the mounting hole. The casing is used for extending and homogenizing the magnetic field of the magnetic element, so that the test tube is under the magnetic field with uniform strength, and the efficiency and the precision of PCR product recovery and purification are improved. In addition, this application still discloses a PCR product recovery purification device.

Description

Magnetic frame for recovering and purifying PCR (polymerase chain reaction) products and recovering and purifying device thereof

Technical Field

The utility model belongs to biological magnetism microballon splitter field especially relates to a magnetic frame and retrieve purification device for purification is retrieved to PCR result.

Background

At present, with the importance of society on new crown blight, PCR purification and extraction technology is increasingly applied and developed. In the PCR purification and extraction technology, a 96-hole magnetic frame can efficiently separate various magnetic beads. Compared with the purification method used by the conventional sequencing company or the laboratory, the method is an alcohol/sodium acetate precipitation method, and the precipitation purification method has the characteristics of long purification time, poor purification effect and the like. The magnetic bead method has the advantages of rapid and simple purification and high recovery rate, can effectively remove fluorescent dye influencing sequencing results, and is increasingly applied by people.

In use, the magnetic beads and the DNA of the sequencing product are mixed together and need to be separated by a magnetic stand. Place 96 hole PCB board in magnetic force frame top, on average can accomplish the process of magnetic separation every thirty seconds to reach the purpose of material such as quick separation purification cell, albumen or nucleic acid, the centrifuging tube of 96 hole magnetic force frame can just hug closely at the magnetic force frame, and the separation process is watched to people's intuitionistic being convenient for. The magnetic frame is usually provided with a magnet for absorbing the magnetic microspheres used for purification and extraction. In nucleic acid isolation and extraction, it is generally applied that magnetic beads containing biochemical modifications are bonded to nucleic acids, and the nucleic acids are adsorbed and purified by the adsorption of a magnet.

However, the existing 96-well magnetic rack usually uses a magnetic strip as a magnetic element for adsorbing magnetic beads. It is laid at magnetic frame bottom along a direction, is located one side of centrifuging tube bottom. For example, the chinese patent discloses a magnetic rack (No. CN 209292326U), in which a magnetic strip group is composed of seven magnets, and is sandwiched between a face plate and a bottom plate, and is parallel to a micro-pore row, and magnetic beads can be adsorbed only from one side of a centrifugal tube. Because the distance between the magnetic strip group and the two sides of the centrifugal tube is different, the strength of the magnetic field in the whole centrifugal tube is different, so that all the magnetic beads in the whole centrifugal tube are difficult to be adsorbed uniformly. This problem results in a portion of the magnetic beads still remaining in the aspirated supernatant and reduces the accuracy of purification.

SUMMERY OF THE UTILITY MODEL

In view of the above-mentioned drawbacks of the related art, an object of the present invention is to provide a magnetic rack and a recovery and purification apparatus for making the intensity of magnetic field in a test tube more uniform, which are used to solve the problems of low purification precision and uneven adsorption strength in the related art.

To achieve the above objects and other related objects, the present application discloses a magnetic rack for recovery and purification of PCR products, comprising: the mounting panel is provided with a bearing surface, and the bearing surface is provided with a mounting hole array which is regularly distributed; the magnetic component comprises a magnetic element and an outer sleeve surrounding the outer side of the magnetic element; each magnetic component is assembled in a single mounting hole of the mounting hole array, and the outer sleeve is embedded between the magnetic element and the mounting hole; and a mounting base plate which abuts against the bottom surface of the mounting panel.

In some embodiments of the present application, the number and the position of the mounting holes in the mounting hole array correspond to the number and the position of the PCR product test tubes to be purified.

In certain embodiments of the present application, the array of mounting holes is adapted for a 96-well plate, and the array of mounting holes is laid out in a 12 × 8 matrix.

In certain embodiments of the present application, the mounting panel comprises: and the positioning part is arranged along at least one side edge of the mounting panel and is used for being matched and positioned with a part above the magnetic frame.

In certain embodiments of the present application, the positioning portion includes: more than one positioning groove, it shrinks to the centre along one side or several side edges of the said installation panel; and/or more than one positioning hole which is sunken downwards at the edge of the mounting panel.

In some embodiments of the present application, the outer sleeve is sleeved outside the magnetic element in parallel with the axis of the magnetic element.

In certain embodiments of the present application, the outer jacket is flush with the magnetic element.

In certain embodiments of the present application, the mounting panel comprises: and the fixing part is arranged at the edge of the mounting panel and is used for connecting the mounting panel with the mounting bottom plate.

In certain embodiments of the present application, the fixing portion includes: more than one screw and screw hole, the screw hole certainly the installation panel extends to mounting plate, the screw is worn to locate the screw hole internal connection the installation panel with mounting plate.

The application also discloses a purification device is retrieved to PCR result, includes: the magnetic frame for recovering and purifying PCR products; a transmission device; the driving device is connected with the magnetic frame through the transmission device; and the control device is electrically connected with the driving device and is used for controlling the driving device to operate.

The utility model has the advantages that:

the application discloses a magnetic force frame for PCR product recovery and purification, which comprises magnetic components corresponding to the positions and the number of test tubes one to one. Each magnetic assembly includes a magnetic element and an outer sleeve located directly below the cuvette. Each jacket is arranged on the outer side parallel to the axis of the magnetic element and is as high as the magnetic element. The jacket is used for extending and homogenizing the magnetic field of the magnetic element, so that the test tube is under the magnetic field with uniform intensity, and the efficiency and the precision of the recovery and purification of the PCR product can be improved.

The utility model discloses a purification device is retrieved to PCR result makes the motor begin to operate, and 96 hole site mounting matrix rises to the assigned position thereupon, provides homogeneous and effectual magnetic field for the PCR result, supplies the purification of PCR result, and this purification process need be gone on many times repeatedly, and 96 hole site mounting matrix can freely rise and descend in the positive and negative rotation in-process of motor in this centre to can provide the magnetic field that specific time specific position intensity is homogeneous in the purification of PCR result, convenient and high-efficient. The magnetic beads adsorbed by the magnetic field are adsorbed on the bottom edge of the test tube in a circular ring shape, the central part of the circular ring is provided with the supernatant, so that a liquid transfer device needle can conveniently absorb the supernatant for liquid transfer or cleaning, the possibility of mistaken adsorption of the magnetic beads is reduced or avoided, the purification accuracy is improved, and impurities such as primer dimers, dNTP, inorganic salts, proteins and the like are effectively removed.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 shows the magnetic frame used in the present application for PCR product recovery and purification.

Fig. 2 is a schematic diagram of a magnetic assembly therein.

Fig. 3 and 4 show perspective views of the purification apparatus.

The reference numbers indicate:

1-installing a panel;

2-a magnetic component;

3, positioning a groove;

4-positioning the hole;

5-a fixed part;

21-a magnetic element;

22-a jacket;

10-a magnetic frame;

20-a transmission device;

30-a drive device;

40-control means.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "upper", "lower", "left", "right", "center", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be a fixed connection, a detachable connection, or an integral connection; can be mechanically or electrically connected; either directly or indirectly through intervening media, or both elements may be in communication. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Also, as used herein, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context indicates otherwise. It will be further understood that the terms "comprises," "comprising," "includes" and/or "including," when used in this specification, specify the presence of stated features, steps, operations, elements, components, items, species, and/or groups, but do not preclude the presence, or addition of one or more other features, steps, operations, elements, components, species, and/or groups thereof. The terms "or" and/or "as used herein are to be construed as inclusive or meaning any one or any combination. Thus, "a, B or C" or "a, B and/or C" means "any of the following: a; b; c; a and B; a and C; b and C; A. b and C ". An exception to this definition will occur only when a combination of elements, functions, steps or operations are inherently mutually exclusive in some way.

The existing 96-well magnetic rack usually adopts a magnetic strip as a magnetic element for adsorbing magnetic beads. It is laid at the bottom of the magnetic frame along one direction and is positioned at one side of the bottom of the centrifugal tube. For example, the chinese patent discloses a magnetic rack (No. CN 209292326U), in which a magnetic strip group is composed of seven magnets, and is sandwiched between a face plate and a bottom plate, and is parallel to a micro-pore row, and magnetic beads can be adsorbed only from one side of a centrifugal tube. Because the distance between the magnetic strip group and the two sides of the centrifugal tube is different, the intensity of the magnetic field in the whole centrifugal tube is different, so that all the magnetic beads in the whole centrifugal tube are difficult to be uniformly adsorbed. This problem results in a portion of the magnetic beads still remaining in the aspirated supernatant and reduces the accuracy of purification.

The inventor of the present application finds that the magnetic stripe composition used in the existing equipment technology is a common technology and a conventional procedure in the PCR recovery, purification and extraction technology, and some people are in doubt and improve the uniformity of the magnetic field. Even if the magnetic stripe is changed into a magnetic column arranged below the test tube, the magnetic field intensity is still difficult to meet the required uniformity.

In view of the above, the present application proposes a magnetic rack for PCR product recovery and purification, comprising: a mounting panel 1, a magnetic assembly 2 and a mounting base plate (not shown). The mounting panel 1 is located above the mounting base plate, and both are abutted against each other. The mounting panel 1 is provided with a bearing surface, and the bearing surface is provided with a mounting hole array which is regularly distributed; the magnetic component 2 comprises a magnetic element 21 and an outer sleeve 22 surrounding the magnetic element 21, each magnetic component 2 is assembled in a single mounting hole of the mounting hole array, and the outer sleeve 22 is embedded between the magnetic element 21 and the mounting hole.

In any of the embodiments provided herein, the magnetic element 21 refers to a substance having magnetic force to generate a magnetic field. Generally, the shape of the magnetic element 21 includes: square magnetic blocks, magnetic columns, round magnetic columns, hollow magnetic columns, round hollow magnetic columns and the like. Correspondingly, the cross section of the magnetic element 21 is in various shapes such as rectangle, circle, hollow circle, etc. Therefore, the shape of the mounting hole and the outer sleeve 22 matched with the magnetic element 21 are matched with the shape of the magnetic element 21.

In any of the embodiments provided herein, the outer casing 22 refers to a metal outer casing 22 capable of conducting magnetic force and extending magnetic field, and the metal material of the outer casing 22 includes a single material metal or alloy having one or more elements of iron, chromium, nickel, etc., and so on.

Referring to fig. 1 and fig. 2, wherein fig. 1 shows a magnetic rack for PCR product recovery and purification according to the present invention, and fig. 2 shows a schematic diagram of a magnetic assembly 2 therein. As shown in fig. 1 and 2, the present application proposes a magnetic rack for PCR product recovery purification, comprising: mounting panel 1, magnetic component 2 and mounting bottom plate. The mounting panel 1 is located above the mounting base plate, and both are abutted against each other. The mounting panel 1 is provided with a bearing surface, and the bearing surface is provided with a mounting hole array which is regularly distributed; the magnetic component 2 comprises a magnetic element 21 and an outer sleeve 22 surrounding the magnetic element 21, each magnetic component 2 is assembled in a single mounting hole of the mounting hole array, and the outer sleeve 22 is embedded between the magnetic element 21 and the mounting hole.

Meanwhile, in some examples, the mounting panel 1 and the mounting base plate may be an integrated base, and in some examples, the mounting panel 1 and the mounting base plate are fixedly connected to form an integrated base.

In the embodiment shown in fig. 1 and 2, the number and positions of the mounting holes in the mounting hole array correspond one-to-one to the PCR product tubes to be purified. Typically, the PCR product tubes are placed in multi-well plates, typically 6-well plates, PCR eight-row tube-in-tube plates, 12-well plates, 24-well plates, 48-well plates, or 96-well plates. In the embodiment shown in FIGS. 1 and 2, the 96-well plate is used as an example, and thus the array of the mounting wells is arranged in a 12X 8 matrix corresponding to the PCR product tubes. One magnetic assembly 2 is mounted in each mounting hole of the mounting hole matrix. The axis of the magnetic component 2 coincides with the center line of the test tube, and the magnetic component 2 generates a magnetic field with uniform intensity below the test tube. If the PCR product test tube to be purified is placed in other multi-well plates such as 12-well plate, 24-well plate, etc., the mounting hole array is arranged in the form of corresponding matrix, the mounting hole matrix and the magnetic component 2 are both arranged below the test tube, and the positions, the number, etc. of the mounting hole matrix are similar and easily associated by the technicians in the field, so that the detailed description is omitted in this embodiment.

In the embodiment shown in fig. 1 and 2, the magnetic element 21 is formed by a circular hollow magnetic column, and an iron outer sleeve 22 is sleeved on the outer side surface in the direction parallel to the axis of the circular hollow magnetic column. That is, the outer sleeve 22 is coaxially fitted with the magnetic element 21, and the height of the outer sleeve 22 in the axial direction is equal to the height of the magnetic element 21. The outer sleeve 22 is made of magnetic conductive metal, and spreads and expands the magnetic field generated by the magnetic element 21 while conducting the magnetic force, so that the magnetic field intensity generated at the bottom of the test tube is more uniform. Simulation software and experiments prove that the number and the density of equivalent magnetic induction lines passing through the bottom of the test tube are more uniform, magnetic beads adsorbed by the magnetic field can be uniformly adsorbed around the bottom of the test tube wall to form a circular ring shape, a liquid transfer device needle can conveniently absorb supernatant in the circular ring to transfer or clean the liquid, the possibility that the magnetic beads are sucked by mistake is reduced or avoided, the purification accuracy is improved, and impurities such as primer dimer, dNTP, inorganic salt, protein and the like are effectively removed.

In some embodiments, the mounting panel 1 comprises: and the positioning part is arranged along at least one side edge of the installation panel 1 and is used for being matched with and positioning a part above the magnetic frame. Generally, the components above the magnet holder include, but are not limited to, perforated plates and other mechanical structures placed above the magnet holder. Since the purification devices developed by companies use different multi-well plate transmission mechanisms, the shapes of the transmission mechanisms and the related positioning mechanisms thereon are difficult to be exhausted in limited embodiments, the parts above the magnetic rack should be understood and interpreted as multi-well plates, mechanisms for transporting multi-well plates, structural outlines on multi-well plates or mechanisms, positioning structures on multi-well plates or mechanisms, and the like.

In the embodiment shown in fig. 1 and 2, the positioning portion includes: more than one positioning groove 3, it shrinks to the centre along one side or several side edges of the said installation panel 1; and, more than one locating hole 4, it is sunken downward on the edge of the said installation panel 1. Wherein, the quantity of positioning groove 3 is two, and two positioning groove 3 set up in both sides edge along 1 length direction at the installation panel respectively, and positioning groove 3 all contracts certain degree of depth to the center. The positioning holes 4 are distributed in the width direction of the installation panel 1, the number of the positioning holes 4 is four, the positioning holes 4 are distributed on the width edge of the installation panel 1 in pairs respectively, and the positioning holes 4 are sunken for a certain depth from the bearing surface of the installation panel 1 to the installation bottom plate. Positioning groove 3 and locating hole 4 all are used for fixing a position with the perforated plate of top assembly and relevant mechanism to ensure the test tube center of perforated plate and the coincidence of the axis of magnetic element 21 of below, guarantee among the test tube intensity homogeneous magnetic field.

In some examples, the mounting panel 1 and the mounting base plate may be an integrated base, and in some examples, the mounting panel 1 and the mounting base plate are fixedly connected to form an integrated base.

In the embodiment shown in fig. 1 and 2, the mounting panel 1 comprises: and the fixing part 5 is arranged at the edge of the installation panel 1, and the fixing part 5 is used for connecting the installation panel 1 and the installation bottom plate. The fixing portion 5 includes: more than one screw and screw hole, the screw hole certainly installation panel 1 extends to mounting plate, the screw is worn to locate the screw downthehole connection installation panel 1 with mounting plate.

In the embodiment shown in fig. 1 and 2, the mounting base plate is made of metal, and the mounting base plate includes: a metal having magnetic conductivity, or a metal plate of a single material or an alloy plate of a plurality of materials of a metal not having magnetic conductivity. The mounting base plate is intended to come into contact with the mounting surface and prevent the magnetic assembly 2 on the mounting panel 1 from falling. Therefore, the installation panel 1 and the installation base plate are abutted against each other or connected to each other to prevent the magnetic assembly 2 from falling.

Based on the above-mentioned magnetic frame that is used for PCR product recovery purification, this application still discloses a PCR product recovery purification device, includes: the magnetic frame 10 for recovering and purifying PCR products; a transmission device 20; a driving device 30 connected with the magnetic frame 10 through the transmission device 20; and a control device 40 electrically connected to the driving device 30 for controlling the driving device 30 to operate.

It should be noted that, in the examples provided in the present application, the transmission device 20 is defined by transmitting the driving force of the driving device 30 to realize the position and range of the porous plate and/or the magnetic rack 10, for example, the transmission mechanism is connected to the output shaft of the driving device 30, and the driving force is converted into the mechanical force of the displacement of the porous plate and/or the magnetic rack 10 by the mechanical structure of the transmission mechanism itself, so as to displace the porous plate and/or the magnetic rack 10.

It should be noted that in the examples provided in the present application, the driving device 30 is defined as a function capable of adjusting the direction and magnitude of the output power under the electrical signal, for example, the driving device 30 can be made of various motors, the driving device 30 outputs the driving force through the output shaft, and the porous plate and/or the magnetic frame 10 is driven to displace through the conversion of the transmission mechanism.

It should be noted that, in each example provided in the present application, the control device 40 is defined as a function of outputting an electrical signal to the driving device 30, for example, the control device 40 may be formed by a PCB such as a single chip, and the control device 40 is electrically connected to the driving device 30, and controls the direction and magnitude of the power output by the driving device 30 by outputting different electrical signal commands, so as to control the position and displacement range of the porous plate and/or the magnetic rack 10. The control device 40 is further capable of communicating with an external instruction source to enable remote control, or the like.

In the embodiment shown in fig. 3 and 4, the magnetic frame 10, the transmission 20, the driving device 30 and the control device 40 are connected in sequence. Specifically, the magnetic rack 10 is disposed above the whole frame of the purification apparatus, a transmission mechanism is installed on the left side of the frame of the purification apparatus, and the frame is provided with a driving device 30 and a control device 40. The transmission device 20 is composed of a speed reducer, a synchronous belt wheel, an idler wheel and a guide rail sliding block, the driving device 30 is composed of a motor, and the control device 40 is composed of a PCB. The slider passes through the keysets and the splint is connected with hold-in range and magnetic force frame 10 simultaneously to the rotary motion with the motor converts the perpendicular up-and-down motion of magnetic force frame 10, thereby controls the distance of magnetic component 2 and perforated plate, and then the magnetic field of the required intensity of control experiment flow.

In summary, the magnetic rack for recovering and purifying PCR products disclosed in the present application includes magnetic components corresponding to the positions and numbers of the test tubes. Each magnetic assembly comprises a magnetic element and an outer sleeve which are positioned right below the test tube. Each jacket is arranged on the outer side parallel to the axis of the magnetic element and has the same height with the magnetic element. The jacket is used for extending and homogenizing the magnetic field of the magnetic element, so that the test tube is under the magnetic field with uniform intensity, and the efficiency and the precision of the recovery and purification of the PCR product can be improved.

The utility model discloses a PCR result recovery purification device makes the motor begin to operate, and 96 hole site mounting matrix rises to the assigned position thereupon, provides homogeneous and effectual magnetic field for the PCR result, supplies the purification of PCR result, and this purification process need be gone on repeatedly many times, and this middle 96 hole site mounting matrix can freely rise and descend in the positive and negative rotation in-process of motor to can provide the magnetic field that specific time specific position intensity is homogeneous in the purification of PCR result, convenient and high-efficient. The magnetic beads adsorbed by the magnetic field are adsorbed on the bottom edge of the test tube in a circular ring shape, the center of the circular ring is provided with the supernatant, so that a liquid shifter needle can conveniently absorb the supernatant for liquid shifting or cleaning, the possibility that the magnetic beads are sucked by mistake is reduced or avoided, the purification accuracy is improved, and impurities such as primer dimers, dNTP, inorganic salts, proteins and the like are effectively removed.

The above description is only for the preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, improvements, equivalents, etc. made within the spirit and principle of the present invention should be included in the scope of the present invention.

Claims (10)

1. A magnetic rack for PCR product recovery purification, comprising:

the mounting panel is provided with a bearing surface, and the bearing surface is provided with a mounting hole array which is regularly distributed;

the magnetic component comprises a magnetic element and an outer sleeve surrounding the outer side of the magnetic element; each magnetic component is assembled in a single mounting hole of the mounting hole array, and the outer sleeve is embedded between the magnetic element and the mounting hole; and

and a mounting base plate which abuts against the bottom surface of the mounting panel.

2. The magnetic rack for recovering and purifying PCR products of claim 1, wherein the number and the positions of the mounting holes in the mounting hole array correspond to the test tubes of the PCR products to be purified one by one.

3. The magnetic rack for recovery and purification of PCR products of claim 2, wherein the mounting hole array is suitable for 96-well plate and is arranged in 12 x 8 matrix.

4. The magnetic rack for PCR product recovery purification of claim 1, wherein the mounting panel comprises: and the positioning part is arranged along at least one side edge of the mounting panel and is used for being matched and positioned with a part above the magnetic frame.

5. The magnetic rack for PCR product recovery and purification of claim 4, wherein the positioning part comprises: more than one positioning groove, it shrinks to the centre along one side or several side edges of the said installation panel; and/or

And the more than one positioning hole is recessed downwards from the edge of the mounting panel.

6. The magnetic rack for recovering and purifying PCR products of claim 1, wherein the outer sleeve is sleeved outside the magnetic element in parallel with the axis of the magnetic element.

7. The magnetic rack for recovering and purifying PCR products of claim 1, wherein the outer sleeve is as high as the magnetic elements.

8. The magnetic rack for PCR product recovery purification of claim 1, wherein the mounting panel comprises: and the fixing part is arranged at the edge of the mounting panel and is used for connecting the mounting panel and the mounting bottom plate.

9. The magnetic rack for PCR product recovery purification of claim 8, wherein the fixing portion comprises: more than one screw and screw hole, the screw hole certainly the installation panel extends to mounting plate, the screw is worn to locate the screw downthehole connection the installation panel with mounting plate.

10. A PCR product recovery and purification device is characterized by comprising:

a magnetic rack for PCR product recovery purification as claimed in any one of claims 1-9;

a transmission device;

the driving device is connected with the magnetic frame through the transmission device; and

and the control device is electrically connected with the driving device and is used for controlling the driving device to operate.

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