CN214991246U - Magnetic separation device of batch purification for cell culture - Google Patents
Magnetic separation device of batch purification for cell culture Download PDFInfo
- Publication number
- CN214991246U CN214991246U CN202022161874.9U CN202022161874U CN214991246U CN 214991246 U CN214991246 U CN 214991246U CN 202022161874 U CN202022161874 U CN 202022161874U CN 214991246 U CN214991246 U CN 214991246U
- Authority
- CN
- China
- Prior art keywords
- pin
- side plate
- hole
- base
- magnetic separation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Abstract
The utility model discloses a cell culture is with magnetic separation device of batch purification, including base, first curb plate, second curb plate and support. The base is provided with a plurality of supporting holes, the hole walls of the supporting holes are provided with magnets, one end of the base is provided with a first embedding groove, the other end of the base is provided with a first embedding convex part corresponding to the first embedding groove, the first side plate and the second side plate are symmetrically arranged and connected to the two ends of the base, the support is connected between the first side plate and the second side plate, and the support is provided with a plurality of fixing grooves corresponding to the supporting holes; furthermore, one side of the base is provided with a plurality of second embedding grooves, and the other side is provided with corresponding second embedding convex parts. When separating numerous samples of quantity, a plurality of these magnetism separator accessible base are around the intercombination, and magnetism separation speed is fast, places the sample cell simultaneously, can satisfy the separation of big batch sample, can make the clear smoothness of whole magnetism separation flow again, is difficult for producing the confusion.
Description
Technical Field
The utility model relates to a biological assay technical field specifically is a magnetic separation device of cell culture with purification in batches.
Background
The magnetic separation device is widely applied to industries such as industrial production, electric power production, food safety, chemical engineering, medical pharmacy and the like at present, and mainly adsorbs iron powder, magnetic beads and the like. The method is mainly used for immune cell sorting, protein, DNA and RNA extraction and purification and the like in the field of molecular biology.
Urine microalbumin is an albumin existing in human urine in large quantity, the measurement of urine microalbumin can reflect the conditions of early nephropathy, kidney injury and the like, and diagnostic reagents used for in vitro diagnosis are mostly composed of monoclonal antibodies. The single effective cell fusion rate under the immunity of the urine microalbumin is high, and the yield rate of monoclonal hybridoma cell strains is also very high. Traditionally, purified antibodies are obtained by inducing ascites in animals or cell culture supernatants. The cycle of obtaining purified antibodies by experimental animals is long and there are uncontrollable factors; the cell culture supernatant has short period and no interference of other globulin. The cell culture supernatant is purified by magnetic beads, the method is convenient and quick, the prior art is limited to the purification of small-batch samples, and how to quickly obtain the specific monoclonal antibodies of a large-batch multi-sample becomes a big problem.
However, the existing commercial magnetic separation device is generally only suitable for magnetic separation operation of small-batch (within 12) centrifuge tubes with standard volume of less than 50ml, and if the types of samples to be processed are more and the volume is smaller, the separation of all samples can be completed through repeated operation for many times, which takes a long time; meanwhile, the situation that the purified magnetic beads are reused to purify the same batch of different samples may exist, which may cause the change of yield and the uncertain cross contamination between antibodies, and seriously affect the development of subsequent experiments.
Those skilled in the art need a detachable magnetic device with controllable number of single-time separation samples, which solves the limitations of the current commercial magnetic devices and is suitable for mass purification of cell culture.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a cell culture is with magnetic separation device of purification in batches to solve the problem among the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
a magnetic separation device for batch purification for cell culture, which comprises a base, a first side plate, a second side plate and a bracket,
the base is provided with a plurality of supporting holes, the wall of each supporting hole is provided with a magnet, one end of the base is provided with a first embedded groove, the other end of the base is provided with a first embedded convex part corresponding to the first embedded groove,
the first side plate and the second side plate are symmetrically arranged and connected at two ends of the base,
the support is connected between the first side plate and the second side plate, and a plurality of fixing grooves corresponding to the supporting holes are formed in the support.
Preferably, one side of the base is provided with a plurality of second embedding grooves, and the other side of the base is provided with second embedding protrusions corresponding to the second embedding grooves.
Preferably, the second engaging recess is recessed downward or inward, and the corresponding second engaging protrusion is raised upward or outward.
Preferably, the first embedding groove is provided with a first bolt hole, and the corresponding first embedding convex part is provided with a second bolt hole;
and/or the second embedding groove is provided with a third bolt hole, and the corresponding second embedding convex part is provided with a fourth bolt hole.
Further, a first stud is connected with the first stud hole and the second stud hole,
and/or a second stud is connected with the third stud hole and the fourth stud hole.
Further, a magnet is connected in the first keyhole,
and/or a magnet is connected in the second keyhole;
a magnet is connected in the third keyhole,
and/or a magnet is connected with the fourth keyhole.
Preferably, one end of the support is provided with a plurality of first pin holes, the other end of the support is provided with a plurality of second pin holes, the first side plate is provided with a plurality of third pin holes corresponding to the first pin holes or the second pin holes, and the second side plate is provided with a plurality of fourth pin holes corresponding to the first pin holes or the second pin holes.
Further, a first pin column is connected with the first pin hole and the third pin hole,
and/or the second pin column is connected with the second pin hole and the fourth pin hole.
Furthermore, a first pin column is connected between the first pin hole at one end of the bracket and the corresponding third pin hole, a magnet is connected in the second pin hole at the other end of the bracket,
or magnets are arranged in the first pin hole at one end of the support and the third pin hole corresponding to the first pin hole, the first pin hole and the third pin hole corresponding to the first pin hole are connected through the magnets, and the magnets are arranged in the second pin hole at the other end of the support.
Furthermore, a magnet is arranged between the first pin hole at the two ends of the bracket and the corresponding third pin hole.
Further, the bracket comprises a first bracket component and a second bracket component which are separated,
one end of the first bracket component is connected with the first side plate through connecting the first pin, or one end of the first bracket component is connected with the first side plate through a magnet,
one end of the second bracket assembly is connected with the second side plate through a second connecting pin, or one end of the second bracket assembly is connected with the second side plate through a magnet.
The utility model discloses there is following beneficial effect:
the utility model discloses a magnetic separation device is provided with the magnet through the supported hole pore wall, base one end is provided with first gomphosis recess, and the other end is provided with corresponding first gomphosis convex part with it, and leg joint is between first curb plate and second curb plate, the support be provided with a plurality of with the corresponding fixed slot of supported hole makes the use the utility model discloses a magnetic separation device at first is minimum one in the quantity of the small volume sample of single separation, and when the quantity of separation sample is many, a plurality of the utility model discloses a magnetic separation device can form multiple specification through the intercombination all around between the base, places the sample cell of demand simultaneously for magnetic separation is fast, can satisfy the separation of big many samples in batches, can make the clear smoothness of whole magnetic separation flow again, is difficult for producing the confusion.
Secondly, because the utility model discloses a centrifuging tube can well be fixed to magnetic separation device's support, the utility model discloses a magnetic separation device can satisfy the operation requirement of constant temperature shaking table, avoids the room temperature to hatch the protein degradation risk that brings, has saved the equipment of hatching that needs supporting device simultaneously.
And thirdly, the utility model discloses a magnetic separation device assembles portably, and convenient to detach reduces idle device's space occupy-place.
Furthermore, the utility model discloses an engineering plastics that magnetic separation device can use, service temperature is wide, and resistant organic matter corrodes, and is light, easy operation.
Drawings
Fig. 1 is a first schematic structural view of an embodiment of a magnetic separation apparatus according to the present invention;
fig. 2 is a second schematic structural view of an embodiment of the magnetic separation apparatus of the present invention;
FIG. 3 is a schematic view of the cross-sectional A-A structure of the magnetic separation device of FIG. 2;
fig. 4 is a schematic structural view of a first side plate of the magnetic separation device of the present invention;
fig. 5 is a schematic structural view of a bracket of the magnetic separation device of the present invention;
fig. 6 is a schematic structural diagram of a first embodiment of a base of a magnetic separation apparatus according to the present invention;
fig. 7 is a schematic structural diagram of a second embodiment of a base of a magnetic separation apparatus according to the present invention;
fig. 8 is a schematic structural diagram of a third embodiment of a base of a magnetic separation apparatus according to the present invention;
fig. 9 is a schematic structural diagram of a fourth aspect of the base of the magnetic separation device of the present invention;
in the figure: 1-base, 2-first side plate, 3-second side plate, 4-bracket, 5-support hole, 6-first embedding groove, 7-first embedding convex part, 8-fixing groove, 9-second embedding groove, 10-second embedding convex part, 11-second bolt, 12-first bolt hole, 13-second bolt hole, 14-first pin hole, 15-third pin hole, 16-third bolt hole, 17-fourth bolt hole and 18-first pin hole.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-9, in an embodiment of the present invention, a batch-purified magnetic separation apparatus for cell culture includes a base 1, a first side plate 2, a second side plate 3, and a support 4,
the base is provided with a plurality of supporting holes 5, the hole walls of the supporting holes 5 are provided with magnets, one end of the base 1 is provided with a first embedded groove 6, the other end is provided with a first embedded convex part 7 corresponding to the first embedded groove,
the first side plate 2 and the second side plate 3 are symmetrically arranged and connected at two ends of the base 1,
the support 4 is connected between the first side plate 2 and the second side plate 3, and the support 4 is provided with a plurality of fixing grooves 8 corresponding to the support holes 5.
The utility model discloses a magnetic separation device uses the polycarbonate material of fibre reinforcement, mainly comprises base 1, support 4, curb plate triplex, and the curb plate divide into first curb plate 2 and second curb plate 3, arranges the bottom plate both sides in, and the base is used for placing the centrifuging tube, adsorbs the magnetic bead, guarantees magnetic means's stability, support 4 for the unsettled part of fixed centrifuging tube. Controlling the cell density, preferably controlling the culture time limit of a single-plant anti-human urine micro-envelope protein monoclonal cell strain to be 4 days, and controlling the collection amount of supernatant to be 40 ml; under the condition of similar cell number and the same culture condition, the quality of the cell strain can be preliminarily judged according to the later acquisition rate of the same batch of antibodies. The size and specification of the centrifuge tube are 50ml, the bracket 4 is used for fixing the centrifuge tube, so that the centrifuge tube can be conveniently taken in batches, and the fixed centrifuge tube device can be placed on a low-temperature shaking table for use; the curb plate cooperation is used support 4 to connect base 1, and the fixed a whole set of magnetic means of equipment can directly be dismantled when idle, and flat-plate is convenient for accomodate, reduces the space occupy-place. The utility model discloses an engineering plastics that magnetic device can use, service temperature is wide, can be able to bear or endure organic substance such as 1640/IMDM complete culture medium and corrode, and is light, easy operation. The magnetic device is provided with a positioning groove, a shaft bolt device and more small magnets, and the positioning groove ensures the stability and uniformity of combination under the assistance of the magnets; the amount of the urine-resistant microalbumin cell culture supernatant is more than 100, and a plurality of magnetic separation devices are combined for use, and can be combined into various specifications through mutual combination all around between the bases 1 to place sample tubes with required amount. The utility model discloses a magnet accessible external force of 1 gomphosis of magnetic separation device's base takes out, is convenient for change, and the base also can be used as ordinary centrifuge tube rack.
Referring to fig. 1, 6, and 8-9, preferably, one side of the base 1 is provided with a plurality of second engaging grooves 9, and the other side is provided with corresponding second engaging protrusions 10; preferably, the second engaging recess 9 is recessed downward or inward, and the corresponding second engaging protrusion 10 is raised upward or outward; aim at, it is a plurality of the utility model discloses a when the magnetic separation device makes up the use, through the all around inter combination of controlling between the base 1 and constitute multiple specification, place the sample cell of demand simultaneously.
Referring to fig. 1, fig. 6-9, preferably, the first engaging recess 6 is provided with a first bolt hole 12, and the corresponding first engaging protrusion 7 is provided with a second bolt hole 13; and/or the second embedding groove 9 is provided with a third bolt hole 16, and the corresponding second embedding convex part 10 is provided with a fourth bolt hole 17; further, a first stud is connected to said first stud hole 12 and said second stud hole 13, and/or a second stud 11 is connected to said third stud hole 16 and said fourth stud hole 17; further, a magnet is connected in the first keyhole 12, and/or a magnet is connected in the second keyhole 13; a magnet is connected in the third keyhole 16 and/or a magnet is connected in the fourth keyhole 17; aim at, the utility model discloses a magnetic means is provided with positioning groove and pintle device to and more small-size magnet, positioning groove guarantee the stability and the homogeneity that combine under the supplementary of magnet.
Referring to fig. 1 to 3, preferably, one end of the bracket is provided with a plurality of first pin holes 14, the other end of the bracket is provided with a plurality of second pin holes, the first side plate 2 is provided with a plurality of third pin holes 15 corresponding to the first pin holes 14 or the second pin holes, and the second side plate 3 is provided with a plurality of fourth pin holes 15 corresponding to the first pin holes 14 or the second pin holes; further, a first pin 18 is connected with the first pin hole 14 and the third pin hole 15, and/or a second pin is connected with the second pin hole and the fourth pin hole; further, a first pin column 18 is connected between the first pin hole 14 at one end of the bracket and the corresponding third pin hole 15, and a magnet is connected in the second pin hole at the other end of the bracket; or magnets are arranged in the first pin hole at one end of the support and the third pin hole corresponding to the first pin hole, the first pin hole and the third pin hole corresponding to the first pin hole are connected through the magnets, and the magnets are arranged in the second pin hole at the other end of the support. Further, one end of the first bracket component is connected with the first side plate by connecting the first pin 18, or one end of the first bracket component is connected with the first side plate by a magnet, one end of the second bracket component is connected with the second side plate by connecting the second pin, or one end of the second bracket component is connected with the second side plate by a magnet. Further, the bracket comprises a first bracket component and a second bracket component which are separated, wherein one end of the first bracket component is connected with the first side plate 2 through connecting the first pin 18, and one end of the second bracket component is connected with the second side plate 3 through connecting the second pin; the purpose is that, on the one hand, the plurality of pin holes and the pin columns of the utility model are internally provided with magnets, or the pin columns are magnets or iron, nickel and other substances which can be adsorbed by the magnets, thereby ensuring the stability and uniformity of combination, the side plates are provided with grooves, the magnets are arranged in the grooves and can be connected with the bracket 4 through magnetic force, and the bracket 4 is matched with the side plates to prevent the inclination of the tube body of the centrifugal tube caused by misoperation; on the other hand, the centrifuging tube device that support 4 was fixed can satisfy the operation requirement of constant temperature shaking table, avoids the room to incubate the protein degradation risk that brings, has saved the equipment of hatching that needs supporting device simultaneously.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. A magnetic separation device for batch purification for cell culture is characterized by comprising a base (1), a first side plate (2), a second side plate (3) and a bracket (4),
the base is provided with a plurality of supporting holes (5), the hole wall of each supporting hole (5) is provided with a magnet, one end of the base (1) is provided with a first embedded groove (6), the other end of the base is provided with a first embedded convex part (7) corresponding to the first embedded groove,
the first side plate (2) and the second side plate (3) are symmetrically arranged and connected at two ends of the base (1),
the support (4) is connected between the first side plate (2) and the second side plate (3), and a plurality of fixing grooves (8) corresponding to the supporting holes (5) are formed in the support (4).
2. A magnetic separation device for cell culture batch purification according to claim 1, characterized in that the base (1) is provided with a plurality of second engaging grooves (9) on one side and a corresponding second engaging protrusion (10) on the other side.
3. A magnetic separation device for cell culture batch purification according to claim 2, wherein the second engaging recess (9) is recessed downward or inward, and the corresponding second engaging protrusion (10) is raised upward or outward.
4. A magnetic separation device for cell culture batch purification according to claim 1, wherein the first fitting recess (6) is provided with a first pin hole (12), and the corresponding first fitting protrusion (7) is provided with a second pin hole (13);
and/or the second embedding groove (9) is provided with a third bolt hole (16), and the corresponding second embedding convex part (10) is provided with a fourth bolt hole (17).
5. A magnetic separation device for cell culture batch purification according to claim 4, wherein a first plug is connected to the first plug hole (12) and the second plug hole (13),
and/or a second stud (11) is connected to the third stud hole (16) and the fourth stud hole (17).
6. A magnetic separation device for mass purification in cell culture according to claim 4, wherein a magnet is attached to the first plug hole (12),
and/or a magnet is connected in the second keyhole (13);
a magnet is connected in the third keyhole (16),
and/or a magnet is connected with the fourth keyhole (17).
7. The magnetic separation device for batch purification in cell culture according to claim 1, wherein one end of the bracket is provided with a plurality of first pin holes (14), the other end of the bracket is provided with a plurality of second pin holes, the first side plate (2) is provided with a plurality of third pin holes (15) corresponding to the first pin holes (14) or the second pin holes, and the second side plate (3) is provided with a plurality of fourth pin holes corresponding to the first pin holes (14) or the second pin holes.
8. A magnetic separation device for cell culture batch purification according to claim 7, wherein a first pin (18) is connected to the first pin hole (14) and the third pin hole (15),
and/or the second pin column is connected with the second pin hole and the fourth pin hole.
9. The magnetic separation device for batch purification in cell culture according to claim 7, wherein a first pin (18) is connected between the first pin hole (14) at one end of the bracket and the corresponding third pin hole (15), and a magnet is connected in the second pin hole at the other end,
or magnets are arranged in the first pin hole (14) at one end of the bracket and the third pin hole (15) corresponding to the first pin hole, the first pin hole (14) and the third pin hole (15) corresponding to the first pin hole are connected through the magnets, and the magnets are arranged in the second pin hole at the other end of the bracket.
10. A magnetic separation device for cell culture batch purification according to claim 9, wherein the rack comprises a first rack component and a second rack component which are separated,
one end of the first bracket component is connected with the first side plate (2) by connecting the first pin (18), or one end of the first bracket component is connected with the first side plate (2) by a magnet,
one end of the second bracket assembly is connected with the second side plate (3) through a second connecting pin, or one end of the second bracket assembly is connected with the second side plate (3) through a magnet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022161874.9U CN214991246U (en) | 2020-09-27 | 2020-09-27 | Magnetic separation device of batch purification for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022161874.9U CN214991246U (en) | 2020-09-27 | 2020-09-27 | Magnetic separation device of batch purification for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214991246U true CN214991246U (en) | 2021-12-03 |
Family
ID=79137787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022161874.9U Active CN214991246U (en) | 2020-09-27 | 2020-09-27 | Magnetic separation device of batch purification for cell culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214991246U (en) |
-
2020
- 2020-09-27 CN CN202022161874.9U patent/CN214991246U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1399718A (en) | Multistage electromagnetic separator for purifying cells, chemicals and protein structures | |
| Sasaki et al. | A palmtop‐sized microfluidic cell culture system driven by a miniaturized infusion pump | |
| CN107209180B (en) | Method for detecting organisms in diluted samples | |
| CN214991246U (en) | Magnetic separation device of batch purification for cell culture | |
| EP3705565A1 (en) | Inclined magnetic holder | |
| CN106754345A (en) | Nucleic acid-extracting apparatus | |
| Su et al. | High-efficiency plasma separator based on immunocapture and filtration | |
| Hu et al. | Large-area electronics-enabled high-resolution digital microfluidics for parallel single-cell manipulation | |
| Lombard-Banek et al. | Mass spectrometry advances and perspectives for the characterization of emerging adoptive cell therapies | |
| CN211471390U (en) | High-flux rapid nucleic acid magnetic bead purification device | |
| CN203355863U (en) | Magnetic separation device for separating biological material | |
| CN202968550U (en) | Nucleic acid purifier | |
| CN107177478B (en) | Three-dimensional microfluidic chip and method for improving cell purity through magnetic separation | |
| CN202063922U (en) | Portable multi-functional magnetic separation device | |
| CN205556697U (en) | Electricity magnetic shelf | |
| Yu et al. | Development of an Efficient, Effective, and Economical Technology for Proteome Analysis | |
| CN102174389A (en) | Portable multi-functional magnetic separation device | |
| CN214150323U (en) | Device for detecting half-sedimentation time of magnetic beads | |
| CN209872968U (en) | Magnetic rack | |
| CN201186244Y (en) | Multifunctional magnetic separation rack having different pore diameters | |
| CN214750345U (en) | Incubation seat | |
| CN217265648U (en) | Nucleic acid extraction device and nucleic acid extraction instrument | |
| CN211999756U (en) | Culture dish for molecular biological experiments | |
| CN210765087U (en) | Purified antibody collection device | |
| CN209685816U (en) | A kind of magnetic frame |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |