CN215209477U - Full-automatic nucleic acid extraction and analysis integrated device - Google Patents
Full-automatic nucleic acid extraction and analysis integrated device Download PDFInfo
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- CN215209477U CN215209477U CN202121719808.7U CN202121719808U CN215209477U CN 215209477 U CN215209477 U CN 215209477U CN 202121719808 U CN202121719808 U CN 202121719808U CN 215209477 U CN215209477 U CN 215209477U
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Abstract
The utility model discloses a full-automatic nucleic acid draws and integrative device of analysis relates to biological liquid sample processing field. Including card box orientation module, magnetism attracts the module, injects the module. The utility model discloses a full-automatic nucleic acid draws and integrative device of analysis with nucleic acid extraction and amplification detection integration, avoids the pollution of nucleic acid extraction in-process, has improved the purity of nucleic acid extraction when accelerating nucleic acid extraction efficiency.
Description
Technical Field
The utility model relates to a biological liquid sample handles field, in particular to full-automatic nucleic acid draws and integrative device of analysis.
Background
With the rapid development of economy and technology, the related research of biomolecules is gradually matured. The separation and purification work related to biomolecules in a laboratory is very important, but the process of purifying and extracting a biomolecule sample is difficult, and not only is a proper purification technology selected, but also a great deal of work is required.
At present, the extraction and detection process of biomolecular nucleic acid by workers is carried out step by step, the main process is divided into four parts, namely pretreatment, nucleic acid extraction, PCR reaction and PCR reaction detection, but the nucleic acid extraction and detection process carried out in a subsection mode needs a large amount of manual participation and wastes time, and pollution is easily caused in the manual participation process.
Therefore, it is necessary to provide a fully automatic integrated nucleic acid extraction and analysis device which integrates nucleic acid extraction and amplification detection, avoids contamination during nucleic acid extraction, and improves the purity of nucleic acid extraction.
SUMMERY OF THE UTILITY MODEL
In order to solve the technical problem, the utility model discloses following technical scheme has been taken:
a fully automatic integrated nucleic acid extraction and analysis device, comprising:
the card box positioning module: the card box positioning module is used for fixing the test card box;
a magnetic attraction module: the magnetic attraction module is used for controlling the movement of the nucleic acid extraction medium;
a bolus module: the injection module is used for injecting the reagent capsule of the test card box.
Further, still include the mount, card box positioning module with magnetism attracts the module all install in on the mount, inject the module with mount sliding connection.
Furthermore, the injection module comprises a lifting platform and an injection device, the lifting platform is connected with the fixing frame in a sliding mode, and the injection device is fixedly connected to the upper end of the lifting platform.
Further, the sample pretreatment device further comprises a blending module, wherein the blending module is connected with the fixing frame, and the blending module is used for sample pretreatment.
Further, still include extensive heating module and PCR precision heating accuse temperature module, extensive heating module install in the elevating platform upper end, extensive heating module is used for heating at the nucleic acid extraction in-process, PCR precision heating accuse temperature module install in card box positioning module one side, PCR precision heating accuse temperature module is used for carrying out the precision accuse temperature heating to the PCR reaction zone of test card box.
Furthermore, the heating material of the PCR precise heating temperature control module adopts one or more of a Peltier material, a precise heating plate and heat-conducting carbon fiber.
Further, the kit also comprises a PCR photoelectric detection recording module, wherein the PCR photoelectric detection recording module is used for performing photoelectric detection on the PCR reaction area of the reagent cartridge.
Furthermore, the device also comprises a main control circuit board which is respectively and electrically connected with the card box positioning module, the PCR photoelectric detection recording module, the magnetic attraction module, the injection module, the extensive heating module and the PCR precise heating temperature control module.
Further, the blending module includes a vibration device.
Further, a heat dissipation mechanism is installed on one side of the extensive heating module.
The beneficial effects of the utility model reside in that:
the utility model discloses with the integration of the extraction detection step of nucleic acid, can once accomplish the nucleic acid pretreatment at the integrative device of full-automatic nucleic acid extraction and analysis, nucleic acid extraction, PCR reaction and PCR reaction detect four steps, save artifically, the external environment who has avoided the nucleic acid extraction in-process pollutes, and handle the nucleic acid in the magnetic ball through magnetism attraction module and the cooperation of injecting the module, and work at every turn can detect a plurality of test card boxes simultaneously, the purity of nucleic acid extraction has been improved when accelerating nucleic acid extraction efficiency.
Drawings
FIG. 1 is a front perspective view of the integrated apparatus for automatic nucleic acid extraction and analysis of the present invention.
FIG. 2 is a perspective view of the rear view of the integrated apparatus for automatic nucleic acid extraction and analysis of the present invention.
FIG. 3 is a side view of the integrated device for fully automatic nucleic acid extraction and analysis of the present invention.
FIG. 4 is a schematic diagram of the PCR photoelectric detection recording module of the full-automatic nucleic acid extraction and analysis integrated device according to the present invention.
FIG. 5 is the utility model relates to a module transmission schematic diagram is injected to integrative device of full-automatic nucleic acid extraction and analysis.
Fig. 6 is the utility model relates to a full-automatic nucleic acid draws and integrative device mixing module sketch map of analysis.
Wherein, in the figure:
101-a base; 102-a first side panel; 103-a second side plate; 104-a first backplane; 105-a second backplane; 201-a first slide rail; 202-a second slide rail; 203-a first slider; 204-a second slider; 205-a skateboard; 206-a first motor; 207-first connection block; 208-a connecting plate; 301-a second motor; 302-a third slide rail; 303-a first pulley; 304-a first drive wheel; 305-a third slider; 306-a photodetector head; 307-connecting the circuit board; 308-a first chute; 309-a second chute; 401-a fourth slide rail; 402-a fifth slide rail; 403-a sixth sliding rail; 404-a seventh sliding rail; 405-a third motor; 406-a fourth motor; 407-a first sliding attachment plate; 408-a second slip joint plate; 409-a third slip joint plate; 410-a fourth slip joint plate; 411-a first magnetic rack; 412-a second magnetic rack; 413-magnetic attraction means; 501-a fifth motor; 502-a cam; 503-cam groove connecting plate; 504-reagent capsule holder; 601-a lifting platform; 602-an eighth slide rail; 603-a ninth slide rail; 604-a lifting slide block; 605-bolus upright; 606-a sixth motor; 607-a second pulley; 608-a second transmission wheel; 701-extensive heating module; 801-precision heating device; an 802-electrical connection socket; 901-master control circuit board.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to fig. 1 to 6 of the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Example one
With reference to fig. 1-4, this embodiment provides a full-automatic integrated nucleic acid extraction and analysis device, which includes a fixing frame, where the fixing frame includes a base 101, a first side plate 102, a second side plate 103, a first back plate 104, and a second back plate 105, the first side plate 102, the second side plate 103, and the first back plate 104 are all vertically installed on an upper end surface of the base 101, the first side plate 102 and the second side plate 103 are parallel to each other, the first back plate 104 is connected to the same side as the first side plate 102 and the second side plate 103, and the second back plate 105 is connected to a top end of the first back plate 104.
The test card box comprises a first side plate 102, a second side plate 103, a card box positioning module, a magnetic attraction module and a pushing module, wherein the card box positioning module is used for fixing the test card box and is connected with the first side plate 102 and the second side plate 103 in a sliding mode; the injection module is used for injecting a reagent capsule of the test cartridge, and the injection module is mounted on one side of the upper end surface of the base 101, which is far away from the first side plate 102 and the second side plate 103; the magnetic attraction module is used for controlling the movement of the nucleic acid extraction medium, and the magnetic attraction module is arranged on two sides of the second back plate 105 and fixedly connected with the second back plate.
The cartridge positioning module comprises a first slide rail 201, a second slide rail 202, a first slide block 203, a second slide block 204, a slide plate 205, a first motor 206, a first connecting block 207 and a connecting plate 208, wherein the first slide rail 201 is fixedly connected to the top end of the first side plate 102, the second slide rail 202 is fixedly connected to the top end of the second side plate 103, the first slide block 203 and the second slide block 204 are mounted at the bottom end of the slide plate 205 and are slidably connected with the first slide rail 201 and the second slide rail 202, and one side of the slide plate 205, which is close to the first back plate 104, is provided with a cartridge slot for fixedly mounting a test cartridge; the connecting plate 208 is installed between the first side plate 102 and the second side plate 103, the first motor 206 is installed at one side of the connecting plate 208 far away from the first back plate 104, the first connecting block 207 is fixedly connected to the bottom end of the sliding plate 205, and the power output end of the first motor 206 is rotatably connected with the first connecting block 207 through a lead screw; when the device normally works, the first motor 206 is started to drive the screw rod to rotate, the first connecting block 207 rotationally connected with the screw rod moves along the axial direction, the first connecting block 207 drives the sliding plate 205 to axially translate, and the axial movement of the test card box is realized.
The kit also comprises a PCR photoelectric detection recording module, wherein the PCR photoelectric detection recording module is used for performing photoelectric detection on a PCR reaction area of the reagent card box; the PCR photoelectric detection recording module is arranged on the upper surface of the cassette positioning module and comprises a second motor 301, a third slide rail 302, a first belt wheel 303, a first driving wheel 304, a third slide block 305, a photoelectric detection head 306 and a connecting circuit board 307; the second motor 301 is mounted on the side of the lower end face of the sliding plate 205, the first pulley 303 is connected to the power output end of the second motor 301, the first driving wheel 304 is rotatably connected to one side, away from the first pulley 303, of the upper surface of the sliding plate 205, the first pulley 303 is connected with the first driving wheel 304 through a driving belt, the bottom end of the third slider 305 is provided with a first sliding chute 308 and a second sliding chute 309 respectively, the third sliding rail 302 is slidably connected with the first sliding chute 308, and the driving belt is arranged in the second sliding chute 309 and fixedly connected with the third slider 305; one end of the connecting circuit board 307 is fixedly connected with the third sliding block 305, and the other end is fixedly connected with the photoelectric detection head 306, and the photoelectric detection head 306 is used for performing photoelectric detection on a corresponding area of the test cartridge; when the device normally works, when a PCR photoelectric detection process needs to be carried out, the second motor 301 drives the first belt wheel 303 to rotate, the transmission belt is driven to follow, the third sliding block 305 fixedly connected with the transmission belt moves along with the transmission belt, the connecting circuit board 307 and the photoelectric detection head 306 are driven to move along the third sliding rail 302, and PCR photoelectric detection is carried out on an object to be detected in the test card box.
The magnetic attraction module comprises a fourth slide rail 401, a fifth slide rail 402, a sixth slide rail 403, a seventh slide rail 404, a third motor 405, a fourth motor 406, a first sliding connection plate 407, a second sliding connection plate 408, a third sliding connection plate 409, a fourth sliding connection plate 410, a first magnetic attraction rack 411, a second magnetic attraction rack 412 and a magnetic attraction device 413, wherein the third motor 405 and the fourth motor 406 are respectively installed on two sides of the second back plate 105, the fourth slide rail 401 and the fifth slide rail 402 are respectively installed on two sides of the third motor 405 and fixedly connected with the second back plate 105, the sixth slide rail 403 and the seventh slide rail 404 are respectively installed on two sides of the fourth motor 406 and fixedly connected with the second back plate 105, the third motor 405 is rotatably connected with the first magnetic attraction rack 411 through a lead screw, the fourth motor 406 is rotatably connected with the second magnetic attraction rack 412 through a lead screw, the top end of the first sliding connection plate 407 is slidably connected to the fourth slide rail 401, the bottom end of the first sliding connection plate 407 is connected to the first magnetic attraction rack 411, the top end of the second sliding connection plate 408 is slidably connected to the fifth slide rail 402, the bottom end of the second sliding connection plate 408 is connected to the first magnetic attraction rack 411, the top end of the third sliding connection plate 409 is slidably connected to the sixth slide rail 403, the bottom end of the third sliding connection plate 409 is fixedly connected to the second magnetic attraction rack 412, the top end of the fourth sliding connection plate 410 is slidably connected to the seventh slide rail 404, and the bottom end of the fourth sliding connection plate 410 is fixedly connected to the second magnetic attraction rack 412; the magnetic attraction devices 413 are uniformly and alternately arranged on the lower end surfaces of the first magnetic rack 411 and the second magnetic rack 412; in the normal work of device, third motor 405 and fourth motor 406 drive the lead screw rotation respectively, inhale the frame 411 and reciprocate with first magnetism of lead screw swivelling joint with second magnetism, inhale the frame 412 and fix magnetism attraction device 413 on first magnetism inhale frame 411 and second magnetism and inhale the frame 412 and follow the removal and send magnetic field, and the removal of cooperation card box orientation module realizes jointly that the magnetic bead in the test card box removes, accomplishes the nucleic acid extraction process in the test card box.
As shown in fig. 1 and fig. 6, the sample pretreatment device further comprises a blending module, wherein the blending module is used for sample pretreatment; the blending module is arranged between the first back plate 104 and the card box positioning module and comprises a fifth motor 501, a cam 502, a cam groove connecting plate 503 and a reagent capsule fixing frame 504, the fifth motor 501 is arranged on one side of the first back plate 104, the cam 502 is connected with the power output end of the fifth motor 501, the cam groove connecting plate 503 is provided with a cam groove, the cam 502 is in sliding connection with the cam groove, and the cam groove connecting plate 503 is fixedly connected with the reagent capsule fixing frame 504; the reagent capsule fixing frame 504 is elastically connected to the top end of the first back plate 104, and the elastic connection mode is that an elastic pad is arranged between the reagent capsule fixing frame 504 and the first back plate, so that the reagent capsule fixing frame 504 has movement amount based on the elastic deformation range of the elastic pad; the working process of the blending module is that the fifth motor 501 drives the cam 502 to rotate, the cam 502 slides relative to the cam groove connecting plate 503 in the rotating process and enables the cam groove connecting plate 503 to be pushed by the protruding part of the cam 502 so as to drive the reagent capsule fixing frame 504 to repeatedly vibrate in a small range, so that the reagents in the fixed reagent capsules are uniformly vibrated, and the pretreatment process of nucleic acid is completed.
As shown in fig. 1 and fig. 5, the bolus module includes a lifting platform 601, an eighth sliding rail 602, a ninth sliding rail 603, a lifting slider 604, a bolus upright 605, a sixth motor 606, a second pulley 607 and a second transmission wheel 608, the eighth sliding rail 602 and the ninth sliding rail 603 are respectively installed on two sides of a surface of the first back plate 104 far away from the fifth motor 501, the eighth slide rail 602 and the ninth slide rail 603 are both provided with the lifting slider 604, the lifting platform 601 is fixedly connected to one side of the two lifting sliders 604, the bolus injection upright column 605 is installed on the upper end surface of the lifting platform 601, the sixth motor 606 is installed at one side of the first back plate 104, the power output end of the sixth motor 606 is connected with the second belt pulley 607, the second transmission wheel 608 is rotatably connected to the upper surface of the base 101, and the second transmission wheel 608 and the second pulley 607 are connected through a transmission belt; the second driving wheel 608 is rotatably connected with the lifting table 601 through a lead screw; when the pushing module works, the sixth motor 606 drives the second belt pulley 607 to rotate, the second belt pulley 607 is connected with the second transmission wheel 608 through a transmission belt to drive the second transmission wheel 608 to rotate, the second transmission wheel 608 drives the screw rod to rotate, and the lifting platform 601 rotationally connected with the screw rod slides up and down and drives the pushing upright post to move up and down.
The heating device further comprises a wide heating module 701, the wide heating module is arranged between the injection upright column 605 and the first back plate 104 and is installed on the upper end surface of the lifting platform 601, in the embodiment, the wide heating module adopts a resistance heating mode, and the position of the wide heating module 701 can be controlled by controlling the lifting platform 601 to slide up and down.
The PCR precise heating temperature control module is used for precisely controlling and heating the temperature of the PCR reaction area of the test card box; PCR precision heating accuse temperature module includes precision heating device 801 and electric connection seat 802, electric connection seat 802 install in slide 205 up end, precision heating device install in electric connection seat one side, precision heating device adopts the Peltier material, precision heating device 801 is used for carrying out the heating of precision accuse temperature to the corresponding part of test card box, provides the accurate temperature that PCR reaction needs.
The heating device further comprises a main control circuit board 901, an article placing groove is formed in the upper surface of the sliding plate 205, the main control circuit board 901 is installed in the article placing groove, and the main control circuit board 901 is electrically connected with the first motor 206, the second motor 301, the third motor 405, the fourth motor 406, the fifth motor 501, the sixth motor 606, the electric connection base 802 and the extensive heating module 701 respectively and controls the working conditions of the workpieces.
The full-automatic nucleic acid extraction and analysis integrated device described in this embodiment normally works as follows, after the device is powered on, the main control circuit board 901 controls the fifth motor 501 to drive the cam 502 to rotate, the cam 502 slides relative to the cam groove connecting plate 503 in the rotating process and enables the cam groove connecting plate 503 to be pushed by the protruding part of the cam 502 to drive the reagent capsule fixing frame 504 to repeatedly vibrate in a small range, so that the reagents in the fixed reagent capsules are uniformly vibrated, and the nucleic acid pretreatment process is completed; after the mixing is finished, the main control circuit board 901 controls the first motor 206 to start to drive the screw rod to rotate, the first connecting block 207 rotationally connected with the screw rod moves along the axial direction, the first connecting block 207 drives the sliding plate 205 to axially translate, and the reagent capsule fixing frame is arranged right above the movable reagent capsule fixing frame of the test card box; the sixth motor 606 is controlled by the main control circuit board 901 to drive the second belt wheel 607 to rotate, the second belt wheel 607 is connected with the second driving wheel 608 through a transmission belt to drive the second driving wheel 608 to rotate, the second driving wheel 608 drives the screw rod to rotate, the lifting platform 601 rotationally connected with the screw rod slides and drives the injection upright post 605 to move, the injection upright post 605 pushes reagent capsules in the capsule fixing frame into the reagent card box, the injection process is the combined action of the card box positioning module and the injection module, the test card box is controlled to slide to enable a plurality of reagent capsules at the bottom end of the test card box to be aligned with the injection upright post 605, and the corresponding reagent capsules of the card box are injected by controlling the upward and downward movement of the injection upright post 605, so that the step of adding various reagents required for nucleic acid extraction is completed; in the process of injecting the medicament each time and the process of finishing injecting, the magnetic attraction module moves up and down and the magnetic attraction device sends out a magnetic field, and the magnetic ball is matched with the axial movement of the card box positioning module to control various movements of the magnetic ball, so that the magnetic ball carries the nucleic acid to move to a PCR reaction area in the test card box after being processed by various reagents, and a wide heating module is used for heating if heating is needed in the process; after the nucleic acid pretreatment and nucleic acid extraction processes are finished, the PCR reaction area is precisely heated through the PCR precise heating temperature control module, and after the heating is finished, each test card box is sequentially scanned and detected through the PCR photoelectric detection recording module, so that a detection result is finally obtained.
Example two
The difference between this embodiment and the first embodiment is that the PCR photoelectric detection recording module is further provided with a signal output line, the signal output line is electrically connected with the main control circuit board 901, and the signal output line is used for transmitting a detection signal.
As optimization, a heat dissipation mechanism is installed on one side of the extensive heating module (701), and in this embodiment, the heat dissipation mechanism adopts a heat dissipation fan for dissipating heat and cooling the test card box.
In order to enable the temperature of the Peltier to be timely and effectively conducted to the PCR reaction pool, one of aluminum, copper, silver and carbon fiber is used for manufacturing a temperature conduction shell, the Peltier is embedded into the temperature conduction shell, the temperature conduction shell is manufactured into a shape matched with the PCR reaction pool, the temperature conduction shell effectively wraps the PCR reaction pool, meanwhile, the surface area of the PCR reaction pool is larger as much as possible under the condition of equal volume, and the PCR reaction pool is more effectively heated.
The bolus injection upright 605 is fixedly connected to the upper end surface of the base 101. The lifting platform 601 only drives the extensive heating module 701 to slide up and down.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to the above-described embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A full-automatic nucleic acid extraction and analysis integrated device is characterized by comprising:
the card box positioning module: the card box positioning module is used for fixing the test card box;
a magnetic attraction module: the magnetic attraction module is used for controlling the movement of the nucleic acid extraction medium;
a bolus module: the injection module is used for injecting the reagent capsule of the test card box.
2. The integrated device for full-automatic nucleic acid extraction and analysis according to claim 1, further comprising a fixing frame, wherein the cartridge positioning module and the magnetic attraction module are both mounted on the fixing frame, and the injection module is slidably connected with the fixing frame.
3. The integrated device for full-automatic nucleic acid extraction and analysis according to claim 2, wherein the bolus module comprises a lifting table and a bolus device, the lifting table is slidably connected with the fixing frame, and the bolus device is fixedly connected to the upper end of the lifting table.
4. The full-automatic nucleic acid extraction and analysis integrated device according to claim 2, further comprising a blending module, wherein the blending module is connected with the fixing frame, and the blending module is used for sample pretreatment.
5. The integrated full-automatic nucleic acid extraction and analysis device according to claim 3, further comprising a broad heating module and a PCR precise heating temperature control module, wherein the broad heating module is installed at the upper end of the lifting platform, the broad heating module is used for heating in the nucleic acid extraction process, the PCR precise heating temperature control module is installed at one side of the cartridge positioning module, and the PCR precise heating temperature control module is used for performing precise temperature control heating on a PCR reaction zone of the test cartridge.
6. The integrated device for full-automatic nucleic acid extraction and analysis according to claim 5, wherein the heating material of the PCR precise heating temperature control module is one or more of a Peltier material, a precise heating plate and a heat-conducting carbon fiber.
7. The integrated apparatus for full-automatic nucleic acid extraction and analysis according to claim 6, further comprising a PCR photodetection recording module for photodetection of the PCR reaction region of the reagent cartridge.
8. The integrated device for full-automatic nucleic acid extraction and analysis according to claim 7, further comprising a main control circuit board electrically connected to the cassette positioning module, the PCR photoelectric detection recording module, the magnetic attraction module, the injection module, the extensive heating module and the PCR precise heating temperature control module, respectively.
9. The integrated device for full-automatic nucleic acid extraction and analysis according to claim 4, wherein the mixing module comprises a shaking device.
10. The integrated apparatus for nucleic acid isolation and analysis according to claim 5, wherein a heat dissipation mechanism is installed on one side of the extensive heating module.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121719808.7U CN215209477U (en) | 2021-07-27 | 2021-07-27 | Full-automatic nucleic acid extraction and analysis integrated device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202121719808.7U CN215209477U (en) | 2021-07-27 | 2021-07-27 | Full-automatic nucleic acid extraction and analysis integrated device |
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| CN215209477U true CN215209477U (en) | 2021-12-17 |
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| CN202121719808.7U Active CN215209477U (en) | 2021-07-27 | 2021-07-27 | Full-automatic nucleic acid extraction and analysis integrated device |
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- 2021-07-27 CN CN202121719808.7U patent/CN215209477U/en active Active
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Effective date of registration: 20220506 Address after: 102200 rooms 302, 303 and 310, floor 3, building 1b, yard 27, Chuangxin Road, science and Technology Park, Changping District, Beijing Patentee after: Beijing Jijian Medical Technology Co.,Ltd. Address before: 215200 No. 168, Ludang Road, Jiangling street, Wujiang District, Suzhou City, Jiangsu Province (north of the original Lingyi Road) Patentee before: Jijian medical technology (Suzhou) Co.,Ltd. |