CN218089556U - Arthrospira culture device - Google Patents
Arthrospira culture device Download PDFInfo
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- CN218089556U CN218089556U CN202222487980.5U CN202222487980U CN218089556U CN 218089556 U CN218089556 U CN 218089556U CN 202222487980 U CN202222487980 U CN 202222487980U CN 218089556 U CN218089556 U CN 218089556U
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Abstract
The utility model provides a arthrodia culture apparatus, arthrodia culture apparatus is including cultivateing jar, pH detecting system, temperature control system, charge pump and material pond, pH detecting system and temperatureThe control system is inserted into the culture tank from the top of the culture tank, a feed hole is formed in the top end of the culture tank, the feed hole is connected with a feed pump and a material pool through a water pipe, a discharge hole and a liquid stop valve are arranged at the bottom of the culture tank, and an LED illumination system and an air inlet unit are arranged at the center of the culture tank. The utility model can adjust the CO to be introduced 2 And the air quantity, the problem of the settlement of the arthrospira is solved, the continuous automatic culture of the arthrospira is realized, the culture efficiency is greatly improved, and the labor cost is reduced.
Description
Technical Field
The utility model belongs to the field is cultivateed to the arthroncus, concretely relates to arthroncus culture apparatus.
Background
The illumination condition has obvious influence on the growth and the accumulation of organic matters of the arthrospira, and the previous researches in laboratories find that under red LED light, the algae strain grows at the fastest speed and accumulates the most biomass, and under blue LED light, the algae strain is more prone to accumulate organic matters such as phycocyanin, protein, polysaccharide and the like. Therefore, a culture device capable of adjusting the combination of illumination intensity, illumination time and red and blue LED light quality is needed to meet different culture requirements, the algae liquid state can be monitored in real time and adjusted in the culture process, and the continuous automatic culture of the arthrospira is realized. And the research in the laboratory finds that CO is properly introduced in the culture process of the arthrospira 2 Can increase the yield of arthrospira by using CO 2 To replace NaHCO 3 The carbon source used as the Zarrouk culture medium for culturing the arthrospira can also reduce the production cost, so that the CO introduction can be adjusted 2 And air quantity equipment, thereby maximally improving the yield of the arthrospira. In the process of cultivating the arthrospira, the algae strains are extremely easy to sink and gather due to gravity, the photosynthesis of the algae strains can be influenced, the growth and the accumulation of organic matters are further influenced, and how to solve the problem of the sedimentation of the arthrospira at low cost in the process of large-scale cultivation is also an urgent problem to be solved.
SUMMERY OF THE UTILITY MODEL
Aiming at the needs of adjusting illumination and CO introduction in the process of culturing the arthrospira 2 And air quantity and algae strain bottom-sinking accumulation. The utility model aims at providing a culture apparatus of arthrodia.
In order to achieve the above purpose, the utility model adopts the following technical scheme to realize:
the utility model provides a culture apparatus of festival spirulina, festival spirulina culture apparatus is including cultivateing jar, PH detecting system, temperature control system, charge pump, material pond and the unit that admits air, PH detecting system and temperature control system insert in the cultivation jar from cultivation tank deck portion, the top of cultivation jar is equipped with the feed port, the feed port leads to pipe to be connected with charge pump and material pond, the bottom of cultivateing the jar is equipped with discharge gate and liquid stop valve the center of cultivation jar is equipped with LED illumination system.
Further, the air inlet unit comprises an aeration disc, the aeration disc is divided into two branches by a guide pipe and is respectively connected with the air pump and the carbon dioxide gas generator, and the two branches are respectively provided with a gas flow meter and a gas stop valve.
Furthermore, the aeration disc is tightly embedded in the culture tank, and 4-10 holes are uniformly formed in the aeration disc.
Furthermore, the LED illumination system is a cylindrical lamp post integrated by LED lamps, and comprises red and blue LED lamps which are arranged at intervals, and each row of lamps are connected in parallel and are mutually independent.
Further, the LED illumination system comprises 40-200 LED lamp beads in red and blue, the red and blue are respectively arranged in 4-10 rows, 10-20 lamps in each row are independent units, and the lamps in each row are connected in series with the slide rheostat.
Further, the LED illumination system is also provided with a timer.
Furthermore, the pH detection system, the temperature control system and the LED illumination system are all connected with the single chip microcomputer through wires.
Further, the culture tank is a vertical cylindrical tank.
Furthermore, the culture device of the arthrospira also comprises a fixed frame, wherein the fixed frame is a cubic bracket made of stainless steel, the upper end and the middle part of the fixed frame are provided with fixed circular rings, and the culture tank is fixedly arranged on the frame.
Furthermore, the fixed frame is provided with an operation table board, the monitoring results of the pH detection system and the temperature control system are displayed on the operation table board in real time, and the operation table board is provided with a gas flowmeter and an LED illumination system regulator.
Compared with the prior art, the utility model has the advantages and beneficial effects that:
1. the utility model discloses a good and comprehensive condition is provided in the cultivation of arthroncus, does not need manual operation to intervene among the cultivation process to realize the continuous automatic cultivation of arthroncus, greatly must promote cultivation efficiency, reduced the cost of labor.
2. The utility model discloses use the LED lamp as the light source, it is energy-conserving high-efficient, the lamp pearl setting is optimized according to the cultivation condition of arthroncus, and control is convenient, can realize the high-efficient cultivation of arthroncus and obtain active product.
3. The utility model discloses at the aeration dish of culture tank bottom installation, in the time of ventilating, rising gas has played the effect of stirring to the algae liquid, makes the algae liquid of gathering again misce bene in the culture medium, has increased the area of contact of algae liquid with the LED light, has improved the holistic photosynthesis of algae liquid, and biomass and organic matter accumulation all have had very big improvement. Compared with the traditional methods such as mechanical stirring and manual stirring, the device is more energy-saving, does not need manual operation, and has lower equipment cost and higher efficiency.
4. The utility model discloses a to the real-time supervision of algae liquid pH, judgement arthrospira's growth state that can be roughly to in time update and adjustment experimental condition.
5. The utility model discloses can continue to increase jar body on this basis, can realize once cultivateing multiple algal column, or as the control experiment of same kind of algae.
Drawings
In order to more clearly explain the technical solution of the culture apparatus of the present invention, the drawings of the culture apparatus will be described.
Fig. 1 is a schematic structural diagram of the present invention;
fig. 2 is a schematic structural diagram of the LED illumination system of the present invention.
In the drawings: 1. an operation table top; 2. a fixed frame; 3. a culture tank; 4, a PH detection system; an LED illumination system; 6. a temperature control system; 7. an aeration disc; 8. a discharge port; 9. a liquid stop valve; 10. a gas flow meter; 11. a gas shutoff valve; 12. an air pump; 13. a carbon dioxide gas generator; 14. a feed pump; 15. and (4) a material tank.
Detailed Description
The following describes in further detail embodiments of the present invention with reference to the accompanying drawings. The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Example 1
The utility model provides a culture apparatus of arthrodia, the culture apparatus of arthrodia is including cultivateing jar 3, PH detecting system 4, temperature control system 6, charge pump 14 and material pond 15, in PH detecting system 4 and temperature control system 6 inserted cultivation jar 3 from cultivation jar 3 tops, the submergence can carry out real-time supervision in the algae liquid. Cultivate jar 3's top and be equipped with the feed port, the feed port leads to pipe and is connected with charge pump 14 and material pond 15, the bottom of cultivating jar 3 is equipped with discharge gate 8 and liquid stop valve 9 the center of cultivating jar 3 is equipped with LED lighting system 5, can provide suitable illumination condition for the cultivation of arthrodia.
The arthrospira culture device further comprises an air inlet unit, the air inlet unit comprises an aeration disc 7, the aeration disc 7 is divided into two branches by a guide pipe and is respectively connected with an air pump 12 and a carbon dioxide gas generator 13, and the two branches are respectively provided with a gas flowmeter 10 and a gas stop valve 11. The air inlet unit can continuously and quantitatively introduce air and CO into the culture tank 3 according to the requirement 2 Or mixing the two according to a certain proportion and introducing.
The aeration disc 7 is tightly embedded in the culture tank 3, and 4-10 holes are uniformly formed in the aeration disc 7. The aeration disc 7 is provided with 8 holes, so that the ascending gas generated by the aeration disc 7 plays a role in stirring the algae liquid and uniformly mixing the precipitated arthrospira.
The LED illumination system 5 is a cylindrical lamp post integrated by LED lamps, and comprises red and blue LED lamps which are arranged at intervals, and each row of lamps are connected in parallel and are mutually independent.
The LED illumination system 5 comprises 40-200 LED lamp beads in red and blue, the red and the blue are respectively arranged in 4-10 rows, and each row comprises 10-20 lamps.The LED illumination system 5 is provided with 100 LED lamp beads in red and blue, the red and the blue are respectively arranged in 5 rows, each row of 20 lamps is an independent unit and is connected with a sliding rheostat in series, the illumination intensity of each row of lamp groups can be controlled through adjustment of a knob, and the actually measured illumination intensity range is 0-96 mu mol/m 2 S, thereby realizing the adjustment of different illumination intensity and red and blue light quality combinations.
The LED illumination system is also provided with a timer, so that the lamp can be turned on and off automatically and timely.
The pH detection system 4, the temperature control system 6 and the LED illumination system 5 are all connected with the single chip microcomputer through leads.
The culture tank 3 is a vertical cylinder tank and is composed of a double-layer transparent acrylic sleeve.
The arthrospira culture device further comprises a fixed frame 2, the fixed frame 2 is a cubic support made of stainless steel, a fixed ring is arranged at the upper end and in the middle of the fixed frame 2, and the culture tank 3 is fixedly installed on the fixed frame 2.
The fixed frame 2 is provided with an operation table board 1, the monitoring results of the pH detection system 4 and the temperature control system 6 are displayed on the operation table board 1 in real time, and the operation table board 1 is provided with a gas flowmeter 10 and an LED illumination system 5 regulator for regulating air inflow and illumination.
Example 2
The utility model is suitable for Arthrospira, such as Arthrospira (Arthrospira), spirulina (Spirulina), etc. The following is the experimental process of selecting and using the device of the utility model.
1. Selecting an Arthrospira platensis strain, adopting a Zarrouk culture medium, setting the culture temperature to be 25 ℃, and setting the illumination period to be L12: d12, the illumination intensity is 25 mu mol/m 2 S, LED light quality combination is red: blue =6:1 and blue: red =6:1, air was pumped into the culture tank with an air pump at a rate of 3L/min, and the LED light quality combinations were red: blue =6:1 and blue: red =6:1, and found significant increases in algal strain biomass and organic matter accumulation over control algal strains grown in erlenmeyer flasks in the laboratory, in red: blue =6:1, biomass, chlorophyll a, phycocyaninThe contents of protein and polysaccharide are respectively improved by 1.9%, 0.2%, 39.6%, 19.2% and 3.8%, and the content of the polysaccharide in the blue: when red =6:1, the contents of biomass, chlorophyll a, phycocyanin, protein and polysaccharide are respectively increased by 9.8%, 4.7%, 29.2%, 11.1% and 25.2%. And the detection results of photosynthetic efficiency parameters Y (II) and NPQ of the algae plants show that the photosynthetic efficiency of the algae plants in the culture device is higher than that of the algae plants in a control group, and the growth state of the algae plants is better.
2. Selecting Arthrospira platensis strain, reducing NaHCO content in Zarrouk culture medium 3 Dosage, introducing CO into the culture tank 2 And air to supplement a carbon source of the original Zarrouk culture medium, wherein the speed of the introduced mixed gas is 3L/min, the culture temperature is set to be 25 ℃, and the illumination period is L12: d12, the illumination intensity is 25 mu mol/m 2 S, the LED light quality combination is red: blue =6:1 and blue: red =6:1, and analysis of experimental results shows that the increase of accumulation of either biomass or organic matter of the arthrospira is significant under the conditions (P)<0.05 In red): when blue =6:1, the contents of biomass, chlorophyll a, phycocyanin, protein and polysaccharide are all improved by 25.2%, 0.8%, 126.1%, 104.8% and 11.8%, respectively, and when the content of blue: when red =6:1, the contents of biomass, chlorophyll a, phycocyanin, protein and polysaccharide are all improved by 41.5%, 6.4%, 28.6%, 35.9% and 25.4%, respectively. Therefore, the utility model discloses can improve the growth of arthroncus and the accumulation of organic matter through coordinating improvement culture medium composition and culture method. The Y (II) value is improved compared with that of conical flask culture, and the photosynthesis efficiency of the algae plants is improved compared with that of a control group, which indicates that the growth state of the algae plants is better. Introducing CO 2 Then not only the carbon source is supplemented, but also the pH value of the culture medium is reduced, and researches show that the Zarrouk culture medium is most suitable for the growth of the arthrospira when the pH value is about 9.0, and the growth of the arthrospira is obviously inhibited when the pH value is higher than 9.5, so that CO is introduced 2 Has growth promoting effect. And CO 2 Replacement NaHCO 3 Is favorable for reducing the production cost.
3. Selecting Arthrospira platensis strain, introducing air into culture tank with complete Zarrouk culture medium, and introducingThe gas velocity is 3L/min, the culture temperature is set to be 25 ℃, the illumination period adopts red light and blue light to alternate for 24 hours for uninterrupted illumination, and the method specifically comprises the following steps: red 6: blue 1 LED lamp was illuminated for 12 hours, converting to blue 6: the LED lamp of red 1 irradiates for 12 hours and continuously circulates, and the illumination intensity is 30 mu mol/m of red LED light 2 S, blue LED light 10. Mu. Mol/m 2 S. From the experimental results, it can be found that compared to the L12: under the cultivation of D12 light period, the continuous cultivation of alternating red and blue lights is compared with that of red: when blue =6:1, the biomass difference is small, the accumulation of chlorophyll a is slightly reduced, but the content of phycocyanin, protein and polysaccharide is obviously improved (P)<0.05 278.7%, 18.3%, 152.0% respectively, contrast blue: when red =6:1, the biomass is significantly increased, which is increased by 29.5%, the content of polysaccharide is not greatly different, the content of chlorophyll a, phycocyanin and protein is reduced, and the growth state of the continuous illumination experimental group is red from the change of Y (II) and NPQ along with the culture time: blue =6:1 and blue: red =6:1, but overall better than the growth state of arthrospira in erlenmeyer flasks, and red-blue continuous light experimental group ratio L12: the culture mode of the D12 light cycle is advanced by at least one week to enter a plateau phase. Therefore, the utility model discloses can change illumination condition and illumination cycle through the cooperation, create a more fast more efficient arthrospira cultivation mode.
As described above, the above embodiments are not limiting embodiments of the present invention, and modifications or equivalent variations made by those skilled in the art based on the substance of the present invention are within the technical scope of the present invention.
Claims (10)
1. The utility model provides a arthroncus culture apparatus which characterized in that: the arthrospira culture device comprises a culture tank, a pH detection system, a temperature control system, a feeding pump, a material pool and an air inlet unit, wherein the pH detection system and the temperature control system are inserted into the culture tank from the top of the culture tank, the top end of the culture tank is provided with a feeding hole, the feeding hole is connected with the feeding pump and the material pool through a water pipe, the bottom of the culture tank is provided with a discharge port and a liquid stop valve, and the center of the culture tank is provided with an LED illumination system.
2. The arthrospira cultivation device according to claim 1, wherein: the air inlet unit comprises an aeration disc, the aeration disc is divided into two branches by a guide pipe and is respectively connected with an air pump and a carbon dioxide gas generator, and the two branches are respectively provided with a gas flow meter and a gas stop valve.
3. The arthrospira cultivation device according to claim 2, wherein: the aeration disc is tightly embedded in the culture tank, and 4-10 holes are uniformly formed in the aeration disc.
4. The arthrospira culture device of claim 1, wherein: the LED illumination system is a cylindrical lamp post integrated by LED lamps, and comprises red and blue LED lamps which are arranged at intervals, and each row of lamps are mutually independent in parallel connection.
5. The arthrospira cultivation device according to claim 4, wherein: the LED illumination system comprises 40-200 LED lamp beads in red and blue, the red and blue are respectively arranged in 4-10 rows, 10-20 lamps in each row are independent units, and the independent units are connected in series with a slide rheostat.
6. The arthrospira cultivation device according to claim 1, wherein: the LED illumination system is also provided with a timer.
7. The arthrospira culture device of claim 1, wherein: and the pH detection system, the temperature control system and the LED illumination system are all connected with the single chip microcomputer through wires.
8. The arthrospira cultivation device according to claim 1, wherein: the culture tank is a vertical cylindrical tank.
9. The arthrospira culture device of claim 1, wherein: the culture device further comprises a fixed frame, the fixed frame is a cubic support made of stainless steel, a fixed circular ring is arranged at the upper end and in the middle of the fixed frame, and the culture tank is fixedly installed on the fixed frame.
10. The arthrospira cultivation device according to claim 9, wherein: the fixed frame is provided with an operation table board, monitoring results of the pH detection system and the temperature control system are displayed on the operation table board in real time, and the operation table board is provided with a gas flowmeter and an LED illumination system regulator.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202222487980.5U CN218089556U (en) | 2022-09-20 | 2022-09-20 | Arthrospira culture device |
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| CN202222487980.5U CN218089556U (en) | 2022-09-20 | 2022-09-20 | Arthrospira culture device |
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