CN215065655U - Multi-factor detection sample pretreatment device - Google Patents
Multi-factor detection sample pretreatment device Download PDFInfo
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- CN215065655U CN215065655U CN202121254662.3U CN202121254662U CN215065655U CN 215065655 U CN215065655 U CN 215065655U CN 202121254662 U CN202121254662 U CN 202121254662U CN 215065655 U CN215065655 U CN 215065655U
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Abstract
The utility model discloses a processing apparatus before multifactor detection sample, organism including built-in electric cabinet, be equipped with work platform on the organism, last liquid feeding work position, the imbibition work position of being equipped with of work platform, still be equipped with the liquid feeding subassembly that is located on the liquid feeding work position on the organism, be located the imbibition subassembly on the imbibition work position. Still be equipped with the double track slide rail on the work platform, it can to slide on the double track slide rail the orifice plate support that moves between liquid feeding work position, the imbibition work position, detachably is connected with the orifice plate that is used for the splendid attire sample on the orifice plate support. The liquid feeding working position is provided with a pore plate shaking table for shaking the pore plate, and the liquid suction working position is provided with a magnetic suction assembly for adsorbing the magnetic ball. The utility model discloses a processing apparatus easy operation before multifactor detection sample has alleviateed operating personnel's working strength, has improved the cleaning efficiency moreover.
Description
Technical Field
The utility model relates to a biological detection technical field especially relates to a processing apparatus before multi-factor detection sample.
Background
The magnetic separation is that nanometer magnetic ball capable of combining with sample is added into separated sample to combine with sample, and the combined magnetic ball is adsorbed onto the wall of container or magnetic rod under the action of magnetic field to realize the separation of target sample from solution system. With the development of flow technologies, the use of flow cytometers is more and more common in China, and the technology for simultaneously detecting multiple factors through different fluorescence-labeled magnetic microspheres is more and more mature. At present, when cytokine is detected, a plate washing machine is usually adopted for matching and cleaning, but because the multi-factor detection adopts a magnetic ball coated antibody, and the magnetic ball is easy to settle and gather at the bottom of a container, the plate washing machine is used, shaking up operation is needed, and because the plate washing machine is not provided with a magnetic device, when a target sample and a solution are separated, the magnetic plate is required to be held by an operator, so that the operation is complex, the workload is large, and the efficiency is lower.
Disclosure of Invention
In order to overcome the defects, the utility model aims to provide a processing apparatus before multi-factor detection sample, easy operation has alleviateed operating personnel's working strength, has improved cleaning efficiency moreover.
In order to achieve the above purpose, the utility model discloses a technical scheme is: a multi-factor detection sample pretreatment device comprises a machine body internally provided with an electric cabinet, wherein a working platform is arranged on the machine body, a liquid adding working position and a liquid absorbing working position are arranged on the working platform, and a liquid adding assembly and a liquid absorbing assembly are also arranged on the machine body, wherein the liquid adding assembly and the liquid absorbing assembly are positioned on the liquid adding working position; still be equipped with the double track slide rail on the work platform, it can to slide on the double track slide rail the orifice plate support that moves between liquid feeding work position, the imbibition work position, detachably is connected with the orifice plate that is used for the splendid attire sample on the orifice plate support. The liquid feeding working position is provided with a pore plate shaking table for shaking the pore plate, and the liquid suction working position is provided with a magnetic suction assembly for adsorbing the magnetic ball.
When the device works, the orifice plate bracket and the orifice plate are moved to a liquid adding working position, and the magnetic ball and the sample are added into the sample hole of the orifice plate; starting the hole plate shaker to shake and mix the hole plate uniformly so as to ensure that the magnetic ball is fully combined with the sample; then the pore plate bracket and the pore plate are moved to a liquid absorption working position through the double-track sliding rail, the magnetic ball combined with the sample is absorbed by the magnetic absorption component, and waste liquid in the sample pore is absorbed by the liquid absorption component; then the orifice plate support and the orifice plate are moved to a liquid feeding working position through the double-track slide rail, washing liquid is added into a sample hole of the orifice plate through the liquid feeding assembly, and then the liquid is uniformly mixed through the orifice plate shaking table and then moved to a liquid absorption working position again to suck the waste liquid, so that the washing of the sample is realized repeatedly.
The beneficial effects of the utility model reside in that: the sample and the magnetic balls can be fully and uniformly shaken by arranging the orifice plate shaking table, so that the magnetic balls are prevented from settling at the bottom of the sample orifice; through setting up the magnetism subassembly of inhaling on work platform can directly cooperate the imbibition subassembly with the waste liquid suction, need not the handheld magnetic plate of operating personnel, and then alleviateed operating personnel's working strength, improved the cleaning efficiency.
Further, the magnetic suction assembly comprises a mounting box fixedly connected to the working platform, a fixing plate capable of moving up and down is arranged in the mounting box, and a plurality of rows of magnetic stripes are arranged on the fixing plate. The upper end of the mounting box is provided with a plurality of holes corresponding to the magnetic strips. When the fixed plate moves upwards, the magnetic strip can penetrate out of the corresponding opening.
Furthermore, an opening is formed in one side of the mounting box, and a connecting plate which extends out of the opening and can move up and down along the opening is arranged on one side of the fixing plate.
Further, a limiting clamping seat is arranged at the position, close to the upper end of the opening, of the mounting box, and a limiting plate capable of being clamped with the limiting clamping seat is hinged to one end, far away from the fixing plate, of the connecting plate. When the fixed plate moves to the top, the limiting plate is rotated to be clamped and fixed with the limiting clamping seat, so that the fixed plate can be limited at the top, and the magnetic stripe can be fully magnetically attracted with the magnetic ball.
Further, the orifice plate comprises an orifice plate body, a plurality of orifice plate through holes for the magnetic strips to pass through are formed in the orifice plate body, and a row of sample holes are formed between every two adjacent orifice plate through holes.
The current magnetic force board is placed in the orifice plate bottom more to make the magnetism ball adsorb in the bottom of orifice plate, in order to prevent that the magnetism ball from being siphoned away by the imbibition subassembly, the imbibition needle of imbibition subassembly stretches into in the sample hole time, can not be too close to the bottom in sample hole, will lead to the waste liquid of sample hole bottom can't be siphoned away like this, influences follow-up operation. Therefore, the magnetic strip is driven to move up and down by the movement of the fixing plate in the mounting box, and then when the magnetic ball needs to be magnetically attracted, the magnetic strip is moved to two sides of the sample hole, so that the magnetic ball can be adsorbed on the side wall of the sample hole, and the liquid absorption assembly is convenient to absorb waste liquid at the bottom of the sample hole; simultaneously, through the reciprocating of magnetic stripe drive magnetic ball reciprocate on the sample hole lateral wall, avoid the waste liquid to gather on the sample hole lateral wall.
Further, one side of the orifice plate body is also provided with a positioning hole for connecting with the orifice plate bracket.
Furthermore, the orifice plate support comprises a connecting beam, and two ends of the connecting beam are respectively provided with a supporting leg capable of sliding on the double-track sliding rail; the connection beam is detachably connected with a support hook used for connecting the pore plate body.
Further, the support hook is established including the cover the tie-beam just can follow the sliding sleeve that the tie-beam removed, the one end an organic whole of sliding sleeve is provided with the extension board that can the bearing live the orifice plate body, be equipped with on the extension board with the reference column that the locating hole matches. The position of the supporting hook can be adjusted according to the size of the pore plate through the movable sliding sleeve, and the pore plate is convenient to take through the positioning column and the positioning hole.
Further, the liquid adding assembly comprises a liquid adding needle plate, and the liquid adding needle plate is connected with a liquid adding bottle through a pipeline; the imbibition subassembly includes the imbibition faller, the imbibition faller passes through the pipeline and is connected with the waste liquid bottle.
Further, the machine body is hinged with a light shield which can be covered on the working platform. Quenching of fluorescence during operation can be prevented by the arrangement of the light shield.
Drawings
Fig. 1 is a schematic structural diagram of an embodiment of the present invention;
fig. 2 is a schematic structural diagram of a working platform according to an embodiment of the present invention;
FIG. 3 is a partial enlarged view of portion A of FIG. 2;
fig. 4 is a schematic structural diagram of an orifice plate holder according to an embodiment of the present invention;
fig. 5 is a schematic structural diagram of a mounting box according to an embodiment of the present invention;
fig. 6 is a schematic structural diagram of a fixing plate according to an embodiment of the present invention.
In the figure:
1-body; 11-a working platform; 12-a double-track slide rail; 13-a light shield; 2-a liquid adding assembly; 21-liquid adding needle plate; 22-liquid adding needle; 3-a wicking component; 31-a liquid suction needle plate; 32-a pipette needle; 4-orifice plate holder; 41-connecting beam; 42-support legs; 43-support hook; 431-a sliding sleeve; 432-an extension plate; 433-positioning column; 5-orifice plate; 51-orifice plate body; 52-perforated plate perforation; 53-sample well; 6-orifice plate shaking table; 7-a magnetic attraction component; 71-mounting a box; 711-opening a hole; 712-an opening; 713-limit card seat; 72-a fixed plate; 721-magnetic strip; 73-a connecting plate; 74-limiting plate.
Detailed Description
The following detailed description of the preferred embodiments of the present invention will be provided in conjunction with the accompanying drawings, so as to enable those skilled in the art to more easily understand the advantages and features of the present invention, and thereby define the scope of the invention more clearly and clearly.
Examples
Referring to the attached drawings 1-6, the utility model discloses a processing apparatus before multifactor detection sample, including the organism 1 of built-in electric cabinet, be equipped with work platform 11 on the organism 1, be equipped with liquid feeding work position, imbibition work position on the work platform 11, still be equipped with liquid feeding subassembly 2 that is located the liquid feeding work position on the organism 1, be located imbibition subassembly 3 on the imbibition work position. Still be equipped with double track slide rail 12 on work platform 11, it can to slide on double track slide rail 12 the orifice plate support 4 that moves between liquid feeding work position, the imbibition work position, detachably is connected with the orifice plate 5 that is used for the splendid attire sample on the orifice plate support 4. The liquid feeding working position is provided with a pore plate shaking table 6 for shaking the pore plate 5, and the liquid suction working position is provided with a magnetic suction component 7 for adsorbing a magnetic ball.
Referring to the attached figure 2, the liquid adding assembly 2 comprises a liquid adding needle plate 21, the liquid adding needle plate 21 is connected with a liquid adding bottle through a pipeline, and a row of liquid adding needles 22 capable of extending into the pore plate 5 are arranged at the bottom end of the liquid adding needle plate 21; imbibition subassembly 3 includes imbibition faller 31, imbibition faller 31 passes through the pipeline and is connected with the waste liquid bottle, the bottom of imbibition faller 31 is equipped with one row and can stretches into the imbibition needle 32 in the orifice plate 5.
When the device works, the orifice plate bracket 4 and the orifice plate 5 are moved to a liquid adding working position, and the magnetic ball and the sample are added into the sample hole of the orifice plate 5; starting the orifice plate shaking table 6 to shake and mix the orifice plate 5 uniformly so that the magnetic ball is fully combined with the sample; then the orifice plate bracket 4 and the orifice plate 5 are moved to a liquid suction working position through a double-track slide rail 12, the magnetic ball combined with the sample is absorbed by a magnetic absorption component 7, and waste liquid in the sample hole is sucked out through a liquid absorption component 3; then the orifice plate support 4 and the orifice plate 5 are moved to a liquid feeding working position through the double-track slide rail 12, washing liquid is added into a sample hole of the orifice plate 5 through the liquid feeding assembly 2, and then the liquid is uniformly mixed through the orifice plate shaking table 6 and then moved to a liquid suction working position again to suck the waste liquid, so that the washing of the sample is repeated for many times.
The current magnetic force board is placed in 5 bottoms on orifice plate more to make the magnetism ball adsorb in 5 bottoms on orifice plate, in order to prevent that the magnetism ball from being siphoned away by imbibition subassembly 3, when imbibition needle of imbibition subassembly 3 stretched into the sample pore, can not be too close to the bottom in sample pore, can lead to the waste liquid of sample pore bottom portion can't be siphoned away like this, influences follow-up operation. Therefore, the present embodiment provides a related improvement on the structure of the magnetic attraction component 7 and the orifice plate 5.
Referring to fig. 5-6, the magnetic attraction assembly 7 includes a mounting box 71 fixedly connected to the working platform 11, the upper end of the mounting box 71 is provided with a plurality of parallel openings 711, and one side of the mounting box 71 is provided with a strip-shaped opening 712. A fixing plate 72 capable of moving up and down is arranged in the mounting box 71, a plurality of rows of magnetic strips 721 corresponding to the openings 711 in a one-to-one manner are fixedly connected to the upper end of the fixing plate 72, and when the fixing plate 72 moves up, the magnetic strips 721 can penetrate through the corresponding openings 711. A connecting plate 73 extending out of the opening 712 and capable of moving up and down along the opening 712 is further fixed to one side of the fixing plate 72.
Since it takes a certain time for the magnetic balls to be coupled with the magnetic stripe 721, it is necessary to fix the magnetic stripe 721 at a position to be sufficiently coupled with the magnetic balls. Accordingly, in the present embodiment, the mounting case 71 is provided with the stopper seat 713 near the upper end of the opening 712, and the stopper plate 74 engageable with the stopper seat 713 is hinged to one end of the connecting plate 73 remote from the fixing plate 72. When the fixing plate 72 moves to the top, the limiting plate 74 is rotated to engage and fix with the limiting clamp seat 713, so that the fixing plate 72 is limited at the top, and the magnetic stripe 721 and the magnetic ball can be fully magnetically attracted. Preferably, a self-locking element may be disposed between the position-limiting plate 74 and the position-limiting clamping seat 713 to improve the stability of the clamping.
Referring to fig. 3, the well plate 5 includes a well plate body 51, a plurality of well plate through holes 52 for the magnetic strips 721 to pass through are formed in the well plate body 51, and a row of sample holes 53 is formed between two adjacent well plate through holes 52. When the well plate 5 is moved to the pipetting station, the magnetic strip 721 can pass through the opening 711, the well plate perforation 52 and flank the sample wells in sequence.
The magnetic strip 721 is driven to move up and down by the movement of the fixing plate 72 in the mounting box 71, so that when the magnetic ball needs to be magnetically attracted, the magnetic strip 721 moves to two sides of the sample hole 53, the magnetic ball can be adsorbed on the side wall of the sample hole 53, and the liquid absorption assembly 3 is convenient to absorb waste liquid at the bottom of the sample hole 53; meanwhile, the magnetic ball is driven to move up and down on the side wall of the sample hole 53 by the up-and-down movement of the magnetic strip 721, so that the waste liquid is prevented from being accumulated on the side wall of the sample hole 53.
In this embodiment, referring to fig. 4, the orifice plate holder 4 includes a connecting beam 41, and supporting legs 42 capable of sliding on the double-rail sliding rails 12 are integrally provided at both ends of the connecting beam 41. A pair of support hooks 43 for connecting the orifice plate body 51 is detachably attached to the connection beam 41. The support hook 43 is established including the cover the sliding sleeve 431 that the tie-beam 41 just can follow the tie-beam 41 and remove, the one end an organic whole of sliding sleeve 431 is provided with the extension board 432 that can hold orifice plate body 51, be equipped with reference column 433 on the extension board 432. One side of the orifice plate body 51 is provided with a pair of positioning holes matched with the positioning columns 433.
First, the positions of the support hooks 43 are adjusted by the movement of the sliding bush 431 on the connecting beam 41 so that the interval between the pair of support hooks 43 matches the size of the orifice plate 5, and then the positioning hole on the orifice plate 5 is aligned with the positioning post 433 and the orifice plate 5 is moved downward so that the positioning post 433 passes through the positioning hole until the lower end of the orifice plate 5 abuts on the extension plate 432. The position of the supporting hook 43 can be adjusted according to the size of the orifice plate 5 through the movable sliding sleeve 431, and the orifice plate 5 is convenient to take and take through the arrangement of the positioning column 433 and the positioning hole.
In this embodiment, the machine body 1 is further hinged with a light shield 13 which can be covered on the working platform 11 through a hinge. Quenching of fluorescence during operation can be prevented by the provision of the light shield 13.
The above embodiments are only for illustrating the technical concept and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, so as not to limit the protection scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be covered in the protection scope of the present invention.
Claims (10)
1. The utility model provides a processing apparatus before multifactor detection sample, includes the organism of built-in electric cabinet, be equipped with work platform on the organism, last liquid feeding work position, the imbibition work position of being equipped with of work platform, its characterized in that: the machine body is also provided with a liquid feeding assembly positioned on the liquid feeding working position and a liquid suction assembly positioned on the liquid suction working position; the working platform is also provided with a double-track slide rail, the double-track slide rail is provided with an orifice plate bracket which can move between the liquid feeding working position and the liquid suction working position in a sliding way, and the orifice plate bracket is detachably connected with an orifice plate for containing a sample; the liquid feeding working position is provided with a pore plate shaking table for shaking the pore plate, and the liquid suction working position is provided with a magnetic suction assembly for adsorbing the magnetic ball.
2. The multi-factor detection sample pre-processing device according to claim 1, wherein: the magnetic suction assembly comprises a mounting box fixedly connected to the working platform, a fixing plate capable of moving up and down is arranged in the mounting box, and a plurality of rows of magnetic stripes are arranged on the fixing plate; the upper end of the mounting box is provided with a plurality of openings corresponding to the magnetic strips; when the fixed plate moves upwards, the magnetic strip can penetrate out of the corresponding opening.
3. The multi-factor detection sample pre-processing device according to claim 2, wherein: an opening is formed in one side of the mounting box, and a connecting plate which extends out of the opening and can move up and down along the opening is arranged on one side of the fixing plate.
4. The multi-factor detection sample pre-processing device according to claim 3, wherein: the mounting box is provided with a limiting clamping seat at the position close to the upper end of the opening, and one end of the connecting plate, which is far away from the fixed plate, is hinged with a limiting plate which can be clamped with the limiting clamping seat.
5. The multi-factor test sample pretreatment apparatus according to any one of claims 2 to 4, wherein: the orifice plate includes the orifice plate body, be equipped with a plurality of confessions on the orifice plate body the orifice plate that the magnetic stripe passed is perforated, adjacent two be equipped with one row of sample hole between the orifice plate perforation.
6. The multi-factor detection sample pre-processing device according to claim 5, wherein: and one side of the pore plate body is also provided with a positioning hole for connecting the pore plate bracket.
7. The multi-factor detection sample pre-processing device according to claim 6, wherein: the pore plate bracket comprises a connecting beam, and supporting legs capable of sliding on the double-track sliding rails are arranged at two ends of the connecting beam respectively; the connection beam is detachably connected with a support hook used for connecting the pore plate body.
8. The multi-factor detection sample pre-processing device according to claim 7, wherein: the support hook is established including the cover the tie-beam just can follow the sliding sleeve that the tie-beam removed, the one end an organic whole of sliding sleeve is provided with the extension board that can the bearing live the orifice plate body, be equipped with on the extension board with the reference column that the locating hole matches.
9. The multi-factor detection sample pre-processing device according to claim 1, wherein: the liquid adding assembly comprises a liquid adding needle plate, and the liquid adding needle plate is connected with a liquid adding bottle through a pipeline; the imbibition subassembly includes the imbibition faller, the imbibition faller passes through the pipeline and is connected with the waste liquid bottle.
10. The multi-factor detection sample pre-processing device according to claim 1, wherein: the machine body is also hinged with a light shield which can be covered on the working platform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121254662.3U CN215065655U (en) | 2021-06-04 | 2021-06-04 | Multi-factor detection sample pretreatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121254662.3U CN215065655U (en) | 2021-06-04 | 2021-06-04 | Multi-factor detection sample pretreatment device |
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| Publication Number | Publication Date |
|---|---|
| CN215065655U true CN215065655U (en) | 2021-12-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121254662.3U Active CN215065655U (en) | 2021-06-04 | 2021-06-04 | Multi-factor detection sample pretreatment device |
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|---|---|
| CN (1) | CN215065655U (en) |
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2021
- 2021-06-04 CN CN202121254662.3U patent/CN215065655U/en active Active
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