CN208033233U - A kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device - Google Patents
A kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device Download PDFInfo
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- CN208033233U CN208033233U CN201721878785.8U CN201721878785U CN208033233U CN 208033233 U CN208033233 U CN 208033233U CN 201721878785 U CN201721878785 U CN 201721878785U CN 208033233 U CN208033233 U CN 208033233U
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- reaction cup
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- cleaning
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Abstract
The utility model is related to a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning devices, belong to magnetic particle cleaning technique field, including the first power plant, cleaning needle fixed plate moving up and down is driven by the first power plant, cleaning needle in cleaning needle fixed plate, reaction cup supporting plate is provided with below the cleaning needle, the holding tank for placing response cup is provided on reaction cup supporting plate, second power plant is installed on reaction cup supporting plate, the line shaft of second power plant is connected with magnet fixed plate, magnet fixed plate is located at the side of holding tank and can be moved up and down under the driving of the second power plant, in magnet fixed plate magnet is equipped with reaction cup corresponding position.The utility model scatters with difficulty after solving the problems, such as magnetic particle aggregation, the advantages of ensure that the clean thorough of magnetic particle cleaning, and then ensure that precision of analysis and stability.
Description
Technical field
The utility model is related to a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning devices, belong to magnetic particle cleaning technique
Field.
Background technology
Magnetic particle refers to that magnetic nano-particle is combined what is formed to be dispersed in certain base fluid with inorganic or organic molecule
In with high stability colloidal state composite material.Since magnetic particle has a magnetic responsiveness, at low cost, less energy consumption and pollution-free etc.
Feature, people are on magnetic particle surface or pass through the functional group on magnetic particle surface(Such as amino, carboxyl, sulfydryl and ethylene oxide)
The bioactive substances such as enzyme, antibody, oligonucleotides are fixed, can be further used for enzyme immobilizatio, target medicine carrier,
Cell sorting, immune detection, albumen and nucleic acid isolate and purify and the fields such as hybridization check.
For traditional immunology detection mostly using ELISA Plate as solid phase carrier, suspension magnetic particle has higher ratio as carrier
Surface area, can be substantially more with example reaction, and the flexible Application of externally-applied magnetic field, has higher compared with ELISA Plate carrier in addition
Sensitivity, faster detection speed and preferably repeatability the advantages that, be widely used at present biology and medicine detection
Equal fields.
Magnetic microparticle chemiluminescence immune assay technological synthesis magnetic particle carrier technique and chemiluminescence immunoassay detection technique,
Keep measurement result more acurrate, it is more stable.In magnetic microparticle chemiluminescence immune assay system, to the cleaning performance of intermediate sediment
Directly affect the accuracy and stability of test result.
Existing cleaning way is as depicted in figs. 1 and 2, and reaction cup 3 needs to be pushed over designated position by push rod when cleaning,
At this point, the magnetic particle in reaction cup 3 is adsorbed by the magnet 6 on delivery chute 2, is driven and cleaned by spindle motor 1 after the completion of to be adsorbed
Needle mounting plate 5 moves down, and the cleaning needle 4 for being mounted on cleaning needle mounting plate 5 as a result, also moves down therewith, while cleaning needle 4
Aspirating needle start in reaction cup 3 liquid carry out imbibition, when cleaning needle 4 is moved to the imbibition of designated position and cleaning needle 4
After needle completes imbibition, injection needle fluid injection into reaction cup 3 of cleaning needle 4, spindle motor 1 drives cleaning needle installation after the completion of fluid injection
Plate 5 and cleaning needle 4 are moved upward to designated position and wait for that magnet 6 adsorbs magnetic particle and complete, and so far primary cleaning is completed, if needing
The above-mentioned action of repetition is cleaned multiple times.Existing cleaning way main problem is that, it is defeated in the cleaning process of magnetic particle
The magnet on slot is sent to always exist suction-operated to magnetic particle, i.e. magnetic field always exists, and since magnetic particle is by magnetic Nano
Particle is combined the minimum particle to be formed with inorganic or organic molecule, after aggregation, it is difficult to scatter, thus leads to existing cleaning
Mode is difficult to thoroughly clean the magnetic particle inside aggregation, it may appear that does not wash clean clearly, thoroughly, and then can cause finally to divide
Analyse fluctuation and the inaccuracy of result.Therefore, how clean thoroughly cleaning is carried out to magnetic particle, to ensure the accurate of analysis result
Property and stability become urgent need to resolve the problem of.
Invention content
To solve the above technical deficiency, it is clear that the utility model provides a kind of magnetic microparticle chemiluminescence immune assay instrument
Cleaning device, difficulty is scattered after solving the problems, such as magnetic particle aggregation, ensure that magnetic particle cleaning it is clean thoroughly, and then ensure that point
Analyse the Stability and veracity of result.
The technical solution of the utility model is as follows:A kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device, including the
One power plant is driven cleaning needle fixed plate moving up and down by the first power plant, in cleaning needle fixed plate
Cleaning needle is provided with reaction cup supporting plate below the cleaning needle, the appearance for placing response cup is provided on reaction cup supporting plate
It receives slot, the second power plant is installed on reaction cup supporting plate, the line shaft of second power plant is connected with magnet fixed plate,
Magnet fixed plate is located at the side of holding tank and can be moved up and down under the driving of the second power plant, in magnet fixed plate and anti-
Glass corresponding position is answered to be equipped with magnet.
The technical solution of the utility model further includes:The magnet fixed plate is provided at both ends with the first mounting hole, reaction
The second mounting hole is provided on saucer plate with the first mounting hole corresponding position, is installed in second mounting hole and the first mounting hole
There is lifting guide pillar.
The technical solution of the utility model further includes:The line shaft is connect by fixed plate with magnet fixed plate, reaction
The back side of saucer plate is provided with original point position part.
The technical solution of the utility model further includes:Back-and-forth motion component, institute are provided with below the reaction cup supporting plate
It includes third power plant to state and be moved forward and backward component, and third power plant is connected with driving member, the driving member with react
Saucer plate connects.
The technical solution of the utility model further includes:The driving member includes the driving wheel connected by driving member, institute
It states and guide pad is connected with by pressing plate on driving member, guide pad is connect by connecting plate with reaction cup supporting plate.
The technical solution of the utility model further includes:It is provided with through-hole on the guide pad, front and back lead is installed in through-hole
The both ends of column, front and back guide post are fixed in mounting base, and the driving wheel is fixed in mounting base.
The technical solution of the utility model further includes:Front and back position limiting structure is provided on the driving member, it is described front and back
Position limiting structure includes catch, and catch is connect with guide pad, and photoelectric sensor is equipped in mounting base.
The technical solution of the utility model further includes:At least two holding tanks are provided on the reaction cup supporting plate, each
The side of holding tank is both provided with the magnet fixed plate driven by the second power plant.
The utility model additionally provides a kind of magnetic particle chemical analyzer cleaning method, mainly includes the following steps that:
Reaction cup is pushed into designated position in the holding tank of reaction cup supporting plate by step 1;
Step 2, start the second power plant band moving magnet fixed plate move up, when in magnet fixed plate magnet with
Reaction cup in holding tank is to after just, the second power plant stopping action, magnet starts to inhale the magnetic particle in reaction cup
It is attached;
Step 3 after the completion of absorption, starts the first power plant and cleaning needle is driven to move down, aspirating needle draws reaction cup
In waste liquid after, injection needle injects cleaning solution into reaction cup;
Step 4 is cleaned while injection needle injects cleaning solution to reaction cup alternatively, needle to be injected is injected to reaction cup
After liquid, starts the second power plant band moving magnet fixed plate and move down, magnet is made to be detached from the magnetic particle in reaction cup;
Step 5 starts the first power plant and cleaning needle is driven to be moved upward to initial position, waits for magnetic particle in cleaning solution
After middle dispersion, startup the second power plant band moving magnet fixed plate, which moves up, adsorbs magnetic particle, completes primary cleaning.
The technical solution of the utility model further includes:In the step 4, when magnet fixed plate move down make magnet with
After magnetic particle is detached from, starts third power plant and drive reaction cup supporting plate front-rear reciprocation movement, keeps magnetic particle fast in cleaning solution
Speed is uniformly dispersed.
The utility model has the beneficial effects that:By separately designing reaction cup supporting plate and magnet fixed plate, to reaction cup
In magnetic particle carry out in cleaning process, magnet fixed plate can relative response saucer plate move up and down, and then can realize that magnet is solid
It magnetic particle absorption or is detached from magnet on fixed board and the reaction cup being placed on reaction cup supporting plate, avoids magnetic in cleaning process
Magnetic particle caused by particle is constantly subjected to magnet adsorption effect assembles, do not wash clean thorough problem clearly, it can be achieved that magnetic particle is poly-
Dispersion after collection, it is ensured that magnetic particle cleans up thoroughly, and then precision of analysis and stability can be improved.
Description of the drawings
Fig. 1 is the structural schematic diagram of the prior art.
Fig. 2 is the schematic diagram of prior art delivery chute.
Fig. 3 is the schematic diagram of the preferred embodiment in the utility model.
Fig. 4 is the bottom substance schematic diagram of the preferred embodiment in the utility model.
Fig. 5 is the front and back position limiting structure schematic diagram of the preferred embodiment in the utility model.
Fig. 6 is the schematic diagram of the utility model magnet fixed plate.
Fig. 7 is the schematic diagram of the utility model cleaning needle.
In figure, the 1, first power plant, 11, cleaning needle fixed plate, 12, cleaning needle, 121, aspirating needle, 122, injection needle,
13, vertical supports, the 2, second power plant, 21, lifting guide pillar, 22, magnet fixed plate, 23, line shaft, 24, fixed plate, 3,
Three power plants, 31, mounting base, 32, driving wheel, 33, connecting plate, 34, reaction cup supporting plate, 341, holding tank, 35, reaction cup,
36, belt, 37, guide pad, 38, front and back guide post, 39, pressing plate, 4, magnet, 5, photoelectric sensor, 6, catch.
Specific implementation mode
Below in conjunction with attached drawing, by embodiment, the utility model is described in further detail.
As shown in Fig. 3 to Fig. 7, the magnetic microparticle chemiluminescence immune assay instrument cleaning device of the utility model includes mainly
Cleaning needle, reaction cup and magnet three parts.
Wherein, cleaning needle part is driven by the first power plant 1, specifically, the first power plant 1 with cleaning needle is installed
12 cleaning needle fixed plate 11 connects, and the first power plant 1 is fixed in vertical supports 13.In cleaning process, it is dynamic to start first
Power apparatus 1 can be selected cylinder, hydraulic cylinder or vertical lift spindle motor, drive cleaning needle fixed plate 11 and mounted on clear
The cleaning needle 12 washed in needle fixed plate 11 moves up.
As shown in fig. 7, cleaning needle 12 includes the aspirating needle 121 that can draw waste liquid in reaction cup, and can be to reaction cup
The injection needle 122 of middle injection cleaning solution.
Magnet segment is driven by the second power plant 2, and specifically, the second power plant 2 is mounted under cleaning needle 12
On the reaction cup supporting plate 34 of side, the line shaft 23 of the second power plant 2 is connect by fixed plate 24 with magnet fixed plate 22, is such as schemed
Shown in 6, magnet 4 is installed in magnet fixed plate 22, for being adsorbed to the magnetic particle in reaction cup 35.In magnet fixed plate
22 bottom centre position is nearby provided with little groove, is used for coordinating with fixed plate 22, and magnet fixed plate 22 is fixed on the
On the line shaft 23 of two power plants 2, with this, under the drive of the second power plant 2, magnet fixed plate 22 can do upwards or to
Under movement, to make magnet 4 thereon be adsorbed or be detached from the magnetic particle in reaction cup 35.
In order to improve the stability that magnet fixed plate 22 moves up and down, lifting guide pillar 21, specifically, lifting guide pillar are devised
21 be mounted on first mounting hole at 22 both ends of magnet fixed plate and on reaction cup supporting plate 34 corresponding position second peace
It fills in hole.
In order to improve the accuracy of the up and down action of magnet fixed plate 22, it is mounted with that origin is fixed at the back side of reaction cup supporting plate 34
Position part, indicates for the origin position to magnet fixed plate 22, can specifically select photoelectric sensor 5 to execute.
It is similar with the first power plant 1, as long as the second power plant 2 can realize that band moving magnet fixed plate 22 moves up and down
, cylinder, hydraulic cylinder or vertical lift spindle motor specifically can be selected.
It includes reaction cup supporting plate 34 to react cup portion, and holding tank 341 is provided on reaction cup supporting plate 34 and is used for placing instead
Answer cup 35, in the embodiments of the present invention, in order to improve the cleaning efficiency of magnetic particle, the quantitative design of holding tank 341 is extremely
Few two, if Fig. 3 show, the quantity of holding tank 341 is three, as a result, one group of reaction cup 35 etc. is to be adsorbed or disengaging
In time, another set reaction cup 35 can be operated, on the basis of simplifying equipment, sharing a set of cleaning needle part,
Can reasonable arrangement each group reaction cup cleaning step, save the time, improve cleaning efficiency.
In order to ensure the cleaning performance of every group of reaction cup 35, it is provided with magnet in the side of each holding tank 341 and fixes
Plate 22 and the second power plant 2 moved up and down with moving magnet fixed plate 22, to ensure in cleaning process, magnet 4 and magnetic
Particle effectively can be adsorbed or be detached from.
In the embodiments of the present invention, in order to improve cleaning efficiency and cleaning performance, before reaction cup portion devises
Moving assembly afterwards, the back-and-forth motion component are driven by third power plant 3, and specifically, third power plant 3 is fixed on positioned at anti-
In the mounting base 31 for answering 34 lower section of saucer plate, third power plant 3 is connect by driving member with reaction cup supporting plate 34, is come with this
Reaction cup supporting plate 34 is driven to be moved forward and backward, it can be achieved that two effects, multiple holding tanks are designed first, working as on reaction cup supporting plate 34
When 341, the reaction cup 35 in different holding tanks 341 can be made with cleaning needle 12 to just, realizing waste liquid using component is moved forward and backward
Draw the injection with cleaning solution;Second, when third power plant 3 drives the 34 fast reciprocating action of reaction cup supporting plate, can pair with
Magnetic particle generates concussion effect after the aggregation that magnet 4 is detached from, and then magnetic particle can be made quickly to be uniformly dispersed in cleaning solution.
As shown in Figure 3 and Figure 4, it is illustrated by taking the driving member that driving wheel 32, belt 36 are constituted as an example.Driving wheel 32 divides
It not being mounted in the mounting base 31 of front-end and back-end, one of them is connect with third power plant 3 for driving wheel, and in addition one
A is driven wheel, is connect with driving wheel by belt 36.Belt 36 and guide pad 37 is set to connect by screw on pressing plate 39, it should
The left and right sides of guide pad 37 is connect with the left and right sides of reaction cup supporting plate 34 respectively by connecting plate 33, as a result, third power
3 priority of device drives reaction cup supporting plate 34 to complete to be moved forward and backward by driving wheel 32, belt 36, guide pad 37, connecting plate 33.
In order to improve reaction cup supporting plate 34 back-and-forth motion stability, devise front and back guide post 38, specifically, front and back guide post
38 pass through the through-hole of guide pad 37 to be fixed in the mounting base 31 of front-end and back-end.
In order to improve the accuracy of 34 fore-aft motion of reaction cup supporting plate, front and back position limiting structure is devised, as shown in figure 5, packet
The photoelectric sensor 5 in front-end and back-end mounting base 31 is included, catch 6 is connected on guide pad 37, when positioned at front end
When photoelectric sensor 5 in mounting base 31 detects catch 6, instruction stopping action being sent out to third power plant 3, it is similar,
When the photoelectric sensor 5 positioned at rear end mounting base 31 detects catch 6, also instruction is sent out to third power plant 3 and stop moving
Make.
The utility model additionally provides magnetic particle chemical analyzer cleaning method, mainly includes the following steps that:
Reaction cup 35 is pushed into designated position in the holding tank 341 of reaction cup supporting plate 34 by step 1;
Step 2 starts the second power plant 2 and is moved up with moving magnet fixed plate 22, the magnetic in magnet fixed plate 22
Reaction cup 35 in iron 4 and holding tank 341 is to after just, 2 stopping of the second power plant action, magnet 4 starts in reaction cup 35
Magnetic particle adsorbed;
Step 3 after the completion of absorption, starts the first power plant 1 and cleaning needle 12 is driven to move down, aspirating needle 121 is drawn
After waste liquid in reaction cup, injection needle 122 injects cleaning solution into reaction cup;
Step 4, while injection needle 122 injects cleaning solution to reaction cup 35, alternatively, needle to be injected 122 is to reaction cup
After 35 injection cleaning solutions, starts the second power plant 2 and moved down with moving magnet fixed plate 22, made in magnet 4 and reaction cup 35
Magnetic particle be detached from;
Step 5 starts the first power plant 1 and cleaning needle 12 is driven to be moved upward to initial position, waits for that magnetic particle is cleaning
After disperseing in liquid, the second power plant 2 of startup is moved up with moving magnet fixed plate 22 adsorbs magnetic particle, completes primary
Cleaning.
If desired it is cleaned multiple times, repeats step 1 to five.
In the utility model, in order to improve cleaning efficiency and cleaning performance, in step 4, when magnet fixed plate 22 is downward
It is mobile to make magnet 4 with after magnetic particle disengaging, start third power plant 3 and drive 34 front-rear reciprocation movement of reaction cup supporting plate, makes magnetic
Particle rapid dispersion in cleaning solution is uniform.
The above is only the preferred embodiment of the utility model, not does limit in any form to the utility model
System, done all any modification, equivalent and improvement etc., should be included in this reality within the spirit and principle of the utility model
Within novel protection domain.
Claims (8)
1. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device, including the first power plant(1), by the first power plant
(1)Drive cleaning needle fixed plate moving up and down(11), be mounted on cleaning needle fixed plate(11)On cleaning needle(12), special
Sign is:The cleaning needle(12)Lower section be provided with reaction cup supporting plate(34), reaction cup supporting plate(34)On be provided with for putting
Set reaction cup(35)Holding tank(341), reaction cup supporting plate(34)On the second power plant is installed(2), second power
Device(2)Line shaft(23)It is connected with magnet fixed plate(22), magnet fixed plate(22)Positioned at holding tank(341)Side and
In the second power plant(2)Driving under can move up and down, magnet fixed plate(22)Upper and reaction cup(35)Corresponding position is equipped with
Magnet(4).
2. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as described in claim 1, it is characterised in that:The magnetic
Ferropexy plate(22)Be provided at both ends with the first mounting hole, reaction cup supporting plate(34)It is upper to be provided with the first mounting hole corresponding position
Second mounting hole is equipped with lifting guide pillar in second mounting hole and the first mounting hole(21).
3. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as claimed in claim 2, it is characterised in that:It is described dynamic
Power axis(23)Pass through fixed plate(24)With magnet fixed plate(22)Connection, reaction cup supporting plate(34)The back side be provided with original point position
Part.
4. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as described in claims 1 to 3 is any, feature exist
In:The reaction cup supporting plate(34)Lower section be provided with back-and-forth motion component, the back-and-forth motion component includes third power dress
It sets(3), third power plant(3)It is connected with driving member, the driving member and reaction cup supporting plate(34)Connection.
5. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as claimed in claim 4, it is characterised in that:The biography
Dynamic component includes the driving wheel connected by driving member(32), pass through pressing plate on the driving member(39)It is connected with guide pad
(37), guide pad(37)Pass through connecting plate(33)With reaction cup supporting plate(34)Connection.
6. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as claimed in claim 5, it is characterised in that:It is described to lead
To block(37)On be provided with through-hole, front and back guide post is installed in through-hole(38), front and back guide post(38)Both ends be fixed on mounting base
(31)On, the driving wheel(32)It is fixed on mounting base(31)On.
7. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as claimed in claim 4, it is characterised in that:The transmission
Front and back position limiting structure is provided on component, the front and back position limiting structure includes catch(6), catch(6)With guide pad(37)Connection,
Mounting base(31)On photoelectric sensor is installed(5).
8. a kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device as claimed in claim 4, it is characterised in that:It is described anti-
Answer saucer plate(34)On be provided at least two holding tanks(341), each holding tank(341)Side be both provided with and moved by second
Power apparatus(2)The magnet fixed plate of driving(22).
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CN201721878785.8U CN208033233U (en) | 2017-12-28 | 2017-12-28 | A kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device |
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CN201721878785.8U CN208033233U (en) | 2017-12-28 | 2017-12-28 | A kind of magnetic microparticle chemiluminescence immune assay instrument cleaning device |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108015071A (en) * | 2017-12-28 | 2018-05-11 | 上海裕隆生物科技有限公司 | A kind of magnetic microparticle chemiluminescence analyzer cleaning device and method |
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2017
- 2017-12-28 CN CN201721878785.8U patent/CN208033233U/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN108015071A (en) * | 2017-12-28 | 2018-05-11 | 上海裕隆生物科技有限公司 | A kind of magnetic microparticle chemiluminescence analyzer cleaning device and method |
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