CN214654887U

CN214654887U - Auxiliary sealing tool suitable for PCR (polymerase chain reaction) combined pipe

Info

Publication number: CN214654887U
Application number: CN202120197367.2U
Authority: CN
Inventors: 李子樵; 王雯怡
Original assignee: Ailex Technology Group Co ltd; Zhejiang Ailex Medical Co ltd
Current assignee: Ailex Technology Group Co ltd; Zhejiang Ailex Medical Co ltd
Priority date: 2021-01-25
Filing date: 2021-01-25
Publication date: 2021-11-09
Anticipated expiration: 2031-01-25

Abstract

The utility model relates to an experimental device technical field discloses an auxiliary seal frock suitable for PCR allies oneself with calandria, including bearing structure and closed apron. Wherein bearing structure includes the backup pad, offers in the backup pad along the vertical direction extension put the thing hole, and PCR allies oneself with the calandria and can wear to locate to put the thing downthehole, and closed apron can support and press PCR allies oneself with the calandria lid on PCR allies oneself with the calandria pipe to make PCR allies oneself with the sealed PCR of calandria lid and allies oneself with the calandria. Under this setting, only need to close the apron and support to press in PCR allies oneself with calandria lid, can realize alliing oneself with the quick closure of calandria to PCR, not only can reduce the manual closed PCR of experimenter and ally oneself with the work load and the time of calandria greatly, still can reduce hand and the contact that PCR allies oneself with the calandria, simple and practical and low cost.

Description

Auxiliary sealing tool suitable for PCR (polymerase chain reaction) combined pipe

Technical Field

The utility model relates to an experimental device technical field especially relates to an auxiliary seal frock suitable for PCR allies oneself with calandria.

Background

The PCR reaction technology is indispensable in the research and medical clinical diagnosis process of molecular biology at present, is widely applied to the fields of heredity, biochemistry, immunity, medicine and the like, is not only applied to basic research such as gene separation, cloning and nucleic sequence analysis, but also can be applied to the diagnosis of diseases or any places with RNA and DNA.

In order to ensure the accuracy of the experiment, the PCR header pipes need to be sealed in the experiment process. At present, it goes on to ally oneself with calandria lid directly to press with the finger to ally oneself with the calandria lid to seal to ally oneself with the calandria, and when the required sample of experiment is more, often need consume more time on sealed antithetical couplet calandria, increased experimenter's health burden, in addition, if PCR allies oneself with the calandria not sealed good, often can influence the experimental result, lead to the experiment failure.

Therefore, an auxiliary sealing tool suitable for a PCR joint-row pipe is urgently needed, the PCR joint-row pipe can be quickly sealed, and the tool is simple, practical and low in cost.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an auxiliary seal frock suitable for PCR allies oneself with calandria, but quick seal PCR allies oneself with calandria, simple and practical and low cost.

As the conception, the utility model adopts the technical proposal that:

the utility model provides an auxiliary seal frock suitable for PCR allies oneself with calandria, includes:

the support structure comprises a support plate, the support plate is provided with an object placing hole extending along the vertical direction, and the PCR row tubes can penetrate through the object placing hole;

and the closed cover plate can be pressed against the PCR row tube cover on the PCR row tube so as to seal the PCR row tube cover.

Furthermore, a convex part is arranged on the closed cover plate and can be pressed against the PCR row tube cover.

Furthermore, a convex part is arranged on the closed cover plate, a bulge is arranged on the PCR gang tube cover, a limit hole is formed in the convex part, and when the closed cover plate is pressed against the PCR gang tube cover, the bulge extends into the limit hole.

Furthermore, the closed cover plate is also provided with an annular side wall, and when the closed cover plate is pressed against the PCR row pipe cover, the PCR row pipe cover is positioned in a space surrounded by the annular side wall.

Further, the closure flap is pivotally connected to the support structure.

Furthermore, the supporting structure further comprises a plurality of side plates arranged below the supporting plate, the side plates and the supporting plate are arranged in an enclosing mode to form an accommodating cavity, and one end of the PCR row tube penetrates through the object placing hole and extends into the accommodating cavity.

Furthermore, a plurality of the side plates are provided with a sinking platform at one end close to the supporting plate, and the supporting plate is supported on the sinking platform.

Furthermore, the article placing holes are provided with a plurality of article placing holes, the supporting plate is further provided with a marking part, and the marking part comprises a first marking part for marking the row information of the article placing holes and a second marking part for marking the column information of the article placing holes.

Further, the auxiliary sealing tool suitable for the PCR exhaust pipe also comprises an accommodating box located below the supporting structure, and the accommodating box is configured to accommodate a cooling device.

Further, the containment box includes at least one drawer-type door.

The utility model has the advantages that:

the utility model provides an auxiliary seal frock suitable for PCR allies oneself with calandria, including bearing structure and closed apron. Wherein bearing structure includes the backup pad, offers in the backup pad along the vertical direction extension put the thing hole, and PCR allies oneself with the calandria and can wear to locate to put the thing downthehole, and closed apron can support and press PCR allies oneself with the calandria lid on PCR allies oneself with the calandria pipe to make PCR allies oneself with the sealed PCR of calandria lid and allies oneself with the calandria. Under this setting, only need to close the apron and support to press in PCR allies oneself with calandria lid, can realize alliing oneself with the quick closure of calandria to PCR, not only can reduce the manual closed PCR of experimenter and ally oneself with the work load and the time of calandria greatly, still can reduce hand and the contact that PCR allies oneself with the calandria, simple and practical and low cost.

Drawings

Fig. 1 is a schematic structural diagram of a closed cover plate according to an embodiment of the present invention;

fig. 2 is a schematic structural diagram of a support plate according to an embodiment of the present invention;

fig. 3 is a schematic structural diagram of an auxiliary sealing tool suitable for a PCR joint-row pipe provided by the embodiment of the present invention.

In the figure:

1. closing the cover plate; 11. an annular sidewall; 12. a convex portion;

2. a support structure; 21. a support plate; 211. a placing hole;

3. an accommodating box.

Detailed Description

In order to make the technical problem solved by the present invention, the technical solution adopted by the present invention and the technical effect achieved by the present invention clearer, the technical solution of the present invention will be further explained by combining the drawings and by means of the specific implementation manner. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting of the invention. It should be further noted that, for the convenience of description, only some but not all of the elements related to the present invention are shown in the drawings.

In the description of the present invention, unless expressly stated or limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, e.g., as meaning permanently connected, detachably connected, or integral to one another; can be mechanically or electrically connected; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.

In the description of the present embodiment, the terms "upper", "lower", "left", "right", and the like are used in the orientation or positional relationship shown in the drawings only for convenience of description and simplicity of operation, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used only for descriptive purposes and are not intended to have a special meaning.

The technical solution of the present invention is further explained by the following embodiments with reference to the accompanying drawings.

As shown in fig. 1-3, the embodiment of the present invention provides an auxiliary sealing tool suitable for a PCR joint-row pipe, which includes a supporting structure 2 and a closed cover plate 1. Wherein bearing structure 2 includes backup pad 21, offers the thing hole 211 of putting that extends along vertical direction on the backup pad 21, and PCR allies oneself with the calandria and can wear to locate to put in the thing hole 211, and closed apron 1 can support and press PCR allies oneself with the calandria lid on PCR allies oneself with the calandria to make PCR ally oneself with the sealed PCR of calandria lid and ally oneself with the calandria. After application of sample at every turn, can connect row's pipe with PCR earlier and place in putting thing hole 211, PCR allies oneself with row's pipe lid and places in PCR that corresponds and ally oneself with row's pipe top, and later with closed apron 1 to press in PCR allies oneself with row's pipe lid again, can realize linking up the quick closure of row's pipe to PCR, this work load and the time that not only can reduce the manual closed PCR of experimenter greatly and ally oneself with the calandria, still can reduce the contact that hand and PCR allied oneself with the calandria, low cost and simple and practical.

Specifically, be equipped with convex part 12 on the closed cover plate 1, convex part 12 can be supported and pressed in PCR bank pipe lid, as shown in fig. 1, be equipped with a plurality of convex parts 12 on the closed cover plate 1, preferably, the position of this convex part 12 and PCR bank pipe one-to-one, this can guarantee to press in PCR bank pipe lid when convex part 12 supports to the PCR bank pipe lid to when making its sealed PCR bank pipe, convex part 12 can be more even to the power of PCR bank pipe lid, so as to avoid PCR bank pipe lid to produce because of the atress is uneven and slide, thereby influence the sealed effect to PCR bank pipe.

Further, because the PCR gang tube is of a plurality of types, namely, a PCR gang tube cover with a bulge at the top is arranged, correspondingly, the closed cover plate 1 is provided with a convex part 12, the convex part 12 is provided with a limit hole, and when the closed cover plate 1 is pressed against the PCR gang tube cover, the bulge extends into the limit hole. It can be understood that, similar to the above, the convex portion 12 can be used to press the PCR row tube cover, but in order to ensure the force application effect of the convex portion 12 on the PCR row tube cover, a limiting hole is opened on the convex portion 12, so that the convex portion 12 can press the plane of the PCR row tube, and the convex portion of the PCR row tube extends into the limiting hole. Of course, the movement of the PCR gang tube cover can be well limited, and the PCR gang tube cover is prevented from moving in a direction parallel to the closed cover plate 1, so that the sealing effect on the PCR gang tube is influenced.

Furthermore, the closed cover plate 1 is further provided with an annular side wall 11, and when the closed cover plate 1 is pressed against the PCR row tube cover, the PCR row tube cover is located in a space surrounded by the annular side wall 11. As shown in fig. 1, for a closed cover 1 with 12 columns and 8 rows, i.e. 96 protrusions 12, it is understood that this corresponds to 12 PCR octal rows. Consequently, be equipped with 12 annular lateral walls 11 on the closed

cover plate

1, 1 annular lateral wall 11 corresponds 1 eight ally oneself with calandria of PCR promptly, and annular lateral wall 11 and PCR ally oneself with the size phase-match of calandria lid, when closed cover plate 1 supports presses in PCR ally oneself with the calandria lid, PCR ally oneself with the calandria lid and just can be located the space that annular lateral wall 11 encloses and establish, also can avoid when closed PCR allies oneself with the calandria like this well, PCR allies oneself with the calandria lid and PCR and allies oneself with the calandria and take place the dislocation.

In addition, in order to ensure the accurate matching between the convex portion 12 on the closing cover plate 1 and the PCR row tube cover, in this embodiment, the closing cover plate 1 is rotatably connected to the supporting structure 2, that is, when the PCR row tube needs to be closed, only the closing cover plate 1 needs to be rotated and pressed on the supporting structure 2, and the relative position relationship between the closing cover plate 1 and the supporting structure 2 does not need to be considered.

As shown in fig. 3, in this embodiment, the supporting structure 2 further includes a plurality of side plates disposed below the supporting plate 21, the side plates and the supporting plate 21 enclose to form an accommodating cavity, and one end of the PCR row tube passes through the object placing hole 211 and extends into the accommodating cavity. Preferably, in the present embodiment, the supporting plate 21 is detachably connected to the plurality of side plates. Of course, in another embodiment, the supporting plate 21 may be only arranged on the side plates. Preferably, in this embodiment, the storage holes 211 are provided with 12 columns and 8 rows, i.e. 96, and the positions and sizes of the storage holes are matched with those of the PCR tubes to be stored. Of course, it can be understood that, because of the limitation of the shape of the PCR row tube itself, when the PCR row tube is inserted into the object placing hole 211, the opening end thereof is necessarily located outside the object placing hole 211, and therefore, it is only necessary to ensure that the height of the accommodating cavity is greater than the length of the PCR row tube which can extend into the accommodating cavity. This also illustrates that the support structure 2 may or may not be configured to include a bottom plate, i.e. the function of the support structure 2 for accommodating the PCR manifold is mainly achieved by means of a plurality of side plates and support plates 21 disposed on the side plates.

Specifically, in the present embodiment, a sinking platform is disposed at one end of each of the side plates close to the support plate 21, and the support plate 21 is supported on the sinking platform. With this arrangement, even if no mechanical connection, such as bolt connection, is provided between the support plate 21 and the side plates, the support plate 21 can be stably disposed on the side plates and will not slide when being subjected to an external force.

As shown in fig. 2, a plurality of storage holes 211 are provided to show a specific position of each storage hole 211. The support plate 21 is further provided with a marking portion, and the marking portion includes a first marking portion for marking the row information of the object placing hole 211 and a second marking portion for marking the column information. Specifically, in the present embodiment, since the article placing holes 211 are provided with 12 rows and 8 columns, correspondingly, the first indication portion includes twelve numeric identifiers, which are 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12, and are arranged horizontally and sequentially from left to right, and the second indication portion includes eight alphabetic identifiers, which are A, B, C, D, E, F, G and H, arranged vertically and sequentially from top to bottom. The first marking part and the second marking part are matched together to generate 96 marking information which can correspond to the 96 object placing holes 211 arranged in the embodiment, so that the PCR row tubes can be marked no matter which object placing hole 211 is placed in.

In addition, because PCR reaction generally need place low temperature environment for a period, consequently, the auxiliary seal frock suitable for PCR allies oneself with calandria that this embodiment provided still includes the holding box 3 that is located bearing structure 2 below, and holding box 3 can be used for holding the heat sink. Preferably, in this embodiment, ice cubes or ice bags can be directly placed in the accommodating box 3, and the PCR connected pipes arranged above the ice cubes or ice bags are cooled through liquefaction and heat absorption of the ice cubes or ice bags.

To facilitate the replacement of the ice cubes or ice bags, the accommodating box 3 includes at least one drawer-type door, which is pushed and pulled to facilitate the insertion and removal of the ice cubes or ice bags. Of course, in another embodiment, the receiving box 3 may be provided with at least one hinged opening/closing door, which saves the operation space for replacing ice cubes or ice bags.

Finally, it should be noted that, because the PCR reaction experiment needs to be protected from light, the material and color capable of shielding light can be selected correspondingly during the manufacturing of the auxiliary sealing tool for the PCR linkage pipe, so as to better guarantee the experimental conditions of the PCR reaction.

The above embodiments have been described only the basic principles and features of the present invention, and the present invention is not limited by the above embodiments, and is not departing from the spirit and scope of the present invention. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. The utility model provides an auxiliary seal frock suitable for PCR allies oneself with calandria which characterized in that includes:

the support structure (2) comprises a support plate (21), the support plate (21) is provided with an object placing hole (211) extending along the vertical direction, and the PCR row tubes can penetrate through the object placing hole (211);

the PCR tube bank cover comprises a closed cover plate (1), wherein the closed cover plate (1) can be pressed against the PCR tube bank cover on the PCR tube bank, so that the PCR tube bank cover seals the PCR tube bank.

2. The auxiliary sealing tool for the PCR (polymerase chain reaction) calandria according to claim 1, characterized in that a convex part (12) is arranged on the closing cover plate (1), and the convex part (12) can be pressed against the PCR calandria cover.

3. The auxiliary sealing tool suitable for the PCR (polymerase chain reaction) calandria according to claim 1, wherein a convex part (12) is arranged on the closing cover plate (1), a protrusion is arranged on the PCR calandria cover, a limiting hole is arranged on the convex part (12), and when the closing cover plate (1) is pressed against the PCR calandria cover, the protrusion extends into the limiting hole.

4. The auxiliary sealing tool for the PCR (polymerase chain reaction) calandria according to any one of claims 2-3, wherein the closed cover plate (1) is further provided with an annular side wall (11), and when the closed cover plate (1) is pressed against the PCR calandria cover, the PCR calandria cover is located in a space surrounded by the annular side wall (11).

5. The auxiliary sealing tool for the PCR gang tube of claim 1, wherein the closing cover plate (1) is rotatably connected to the support structure (2).

6. The auxiliary sealing tool suitable for the PCR (polymerase chain reaction) row tube is characterized in that the supporting structure (2) further comprises a plurality of side plates arranged below the supporting plate (21), the side plates and the supporting plate (21) are enclosed to form an accommodating cavity, and one end of the PCR row tube penetrates through the object placing hole (211) and extends into the accommodating cavity.

7. The auxiliary sealing tool suitable for the PCR (polymerase chain reaction) tube bank is characterized in that a sinking platform is arranged at one end of each side plate close to the support plate (21), and the support plate (21) is supported on the sinking platform.

8. The auxiliary sealing tool suitable for the PCR (polymerase chain reaction) tube bank is characterized in that a plurality of object placing holes (211) are arranged, a marking part is further arranged on the supporting plate (21), and the marking part comprises a first marking part for marking the row information and a second marking part for marking the column information of the object placing holes (211).

9. The auxiliary sealing tool for the PCR (polymerase chain reaction) row tube is characterized by further comprising a containing box (3) positioned below the supporting structure (2), wherein the containing box (3) is configured to contain a cooling device.

10. The auxiliary sealing tool for the PCR co-extrusion pipe as claimed in claim 9, wherein the accommodating box (3) comprises at least one drawer type door.