CN210437658U - High-flux tumor cell three-dimensional culture and drug screening kit - Google Patents
High-flux tumor cell three-dimensional culture and drug screening kit Download PDFInfo
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- CN210437658U CN210437658U CN201921001080.7U CN201921001080U CN210437658U CN 210437658 U CN210437658 U CN 210437658U CN 201921001080 U CN201921001080 U CN 201921001080U CN 210437658 U CN210437658 U CN 210437658U
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Abstract
The utility model discloses a three-dimensional culture of high flux tumour cell and medicine screening kit, concretely relates to kit field, including the kit body, kit body top is equipped with first storage tank, first storage tank one side is equipped with the second storage tank, first storage tank one end is equipped with the fixed slot, first storage tank inside is equipped with the culture plate, second storage tank inside is equipped with the reagent bottle, the inside reagent pipe that is equipped with of fixed slot, reagent bottle and reagent pipe outer wall all are equipped with label paper, still be equipped with the plastic-aluminum bag in the first storage tank. The utility model discloses a be equipped with and cultivate board and plastic-aluminum bag, the user can adopt respectively to cultivate board and plastic-aluminum bag when preserving the culture solution under 4 ℃ and-20 ℃ environment, and square big reagent bottle can be stored to the second storage tank, and a plurality of fixed slots are used for saving many reagent pipes, high flux, stable, even, controllable formation tumour microballon.
Description
Technical Field
The utility model relates to a kit technical field, more specifically say, the utility model relates to a three-dimensional culture of high flux tumor cell and drug screening kit.
Background
In a work published in the 2010 Nature Medicine journal, scores of a plurality of tumor models are compared, and the score of a two-dimensional model is zero, which represents that the two-dimensional tumor model can not simulate the development of a real tumor under the three-dimensional condition. The three-dimensional culture of the cells can more accurately simulate the microenvironment where the tumor cells are located, and the three-dimensional tumor cell balls can naturally simulate the inherent metabolism (oxygen, carbon dioxide, nutrients and waste) of the tumor, so that the three-dimensional tumor cell balls are good physiological tumor models. Therefore, the three-dimensional cell experiment is adopted in the research and development process of the tumor drug, so that the drug screening efficiency can be improved, and the research and development cost of the drug can be reduced.
At present, the three-dimensional culture method of the cell microspheres is multiple and mainly comprises two modes of solid carrier culture and liquid carrier culture, and the U-shaped bottom method based on the liquid carrier culture is a method which is simple to operate, controllable in balling and capable of realizing high flux. However, from three-dimensional cell ball culture to realization of a drug screening function, a matrix suitable for cell growth and migration and a reagent for detecting cell survival rate and migration rate after drug screening are needed, the whole process needs various reagents and consumables, some reagents need to be used for multiple times, and storage conditions are different. For the reasons, most users have difficulty in rapidly purchasing complete required reagent consumables or selecting a matrix and a detection reagent suitable for tumor cell growth, and no kit for realizing three-dimensional culture of tumor microspheres by a U-shaped bottom method or culture and detection integration is available at present.
SUMMERY OF THE UTILITY MODEL
In order to overcome prior art's above-mentioned defect, the embodiment of the utility model provides a high flux tumour cell three-dimensional culture and drug screening kit, preserve the reagent through adopting culture plate and plastic-aluminum bag under 4 ℃ and-20 ℃ environment respectively, square big reagent bottle can be stored to the second storage tank, a plurality of fixed slots are used for saving many reagent pipes, adopt U type plate, the real-time formation of image analytical instrument of cooperation high-content three-dimensional tumour cell, utilize centrifugal method to reach the cell balling-up purpose, high flux, it is stable, even, controllable formation tumour microballon, realize external three-dimensional culture, and carry out tumour growth, the detection of important index such as metastasis.
In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a three-dimensional cultivation of high flux tumour cell and drug screening kit, includes the kit body, kit body top is equipped with first storage tank, first storage tank one side is equipped with the second storage tank, first storage tank one end is equipped with the fixed slot, first storage tank inside is equipped with the culture plate, the inside reagent bottle that is equipped with of second storage tank, the inside reagent pipe that is equipped with of fixed slot, reagent bottle and reagent pipe outer wall all are equipped with label paper, still be equipped with the plastic-aluminum bag in the fixed slot, the reagent pipe top outside is equipped with the connecting cover, the connecting cover middle part is equipped with the observation window, the connecting cover inner wall is equipped with sealed the pad.
In a preferred embodiment, the number of the fixing grooves is set to be plural, and the plural fixing grooves and the second storage groove each penetrate the cartridge body longitudinally.
In a preferred embodiment, the aluminum-plastic bag and the reagent tube are both adapted to the fixing groove.
In a preferred embodiment, the kit body is made of sponge material, and a packing box is arranged outside the kit body and the aluminum plastic bag, and the packing box is an airplane box type packing box.
In a preferred embodiment, the label paper on the reagent bottle and the reagent tube is arranged at a height greater than the thickness of the reagent kit body.
In a preferred embodiment, the reagent bottle is made of polyvinyl chloride, and the reagent tube is made of polypropylene.
In a preferred embodiment, the number of the reagent tubes is provided in plurality, and the plurality of the reagent tubes includes both transparent and brown.
In a preferred embodiment, the back adhesive of the label paper is low temperature resistant back adhesive, and the label paper is printed by using a carbon tape.
In a preferred embodiment, the observation window is integrally formed with the connecting cover, the observation window is arranged corresponding to the position of the label paper, and the connecting cover is frosted.
The utility model discloses a technological effect and advantage:
1. the utility model discloses a be equipped with culture plate and plastic-aluminum bag, the user can adopt culture plate and plastic-aluminum bag to preserve the reagent respectively under 4 ℃ and-20 ℃ environment, the square big reagent bottle can be stored to the second storage tank, a plurality of fixed slots are used for storing many reagent pipes, adopt U template method, cooperate high-content three-dimensional tumor cell real-time imaging analytical instrument, utilize centrifugal method to reach the cell balling-up purpose, high flux, stable, even, controllable formation tumor microsphere, realize external three-dimensional culture, and carry out the detection of important indexes such as tumor growth, metastasis;
2. the utility model discloses a be equipped with the connecting cover, will connect the cover and overlap in the reagent pipe outside, the sealed degree of sealing between multiplicable connecting cover of filling up and the reagent pipe avoids the liquid outflow to cause the typeface on the label paper fuzzy, and the user can be observed typeface on to the label paper by the observation window, avoids taking place the chaotic condition of sample and takes place.
Drawings
Fig. 1 is a schematic view of the overall structure of the first storage mode of the present invention.
Fig. 2 is a schematic diagram of the structure of the kit body of the present invention.
Fig. 3 is a top view of the kit body of the present invention.
Fig. 4 is a side view of the kit body of the present invention.
Fig. 5 is a schematic diagram of the reagent tube structure of the present invention.
Fig. 6 is a schematic view of the connecting cover structure of the present invention.
The reference signs are: the kit comprises a kit body 1, a first storage tank 2, a second storage tank 3, a fixed tank 4, a culture plate 5, a reagent bottle 6, a reagent tube 7, label paper 8, a connecting cover 9, an observation window 10 and a sealing gasket 11.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model provides a high-flux tumor cell three-dimensional culture and drug screening kit, which comprises a kit body 1, wherein the top of the kit body 1 is provided with a first storage tank 2, one side of the first storage tank 2 is provided with a second storage tank 3, one end of the first storage tank 2 is provided with a fixed groove 4, the inside of the first storage tank 2 is provided with a culture plate 5, the inside of the second storage tank 3 is provided with a reagent bottle 6, the inside of the fixed groove 4 is provided with a reagent tube 7, the outer walls of the reagent bottle 6 and the reagent tube 7 are both provided with label paper 8, the inside of the fixed groove 4 is also provided with an aluminum-plastic bag (not shown in the figure), the number of the fixed grooves 4 is multiple, the fixed grooves 4 and the second storage tank 3 vertically penetrate through the kit body 1, the aluminum-plastic bag and the reagent tube 7 are both adapted with the fixed groove 4, the second storage tank 3 is adapted with the reagent bottle 6, the kit body 1 is made by the sponge material, kit body 1 and the aluminium-plastic bag outside are equipped with the packing carton, the packing carton adopts aircraft box-shaped packing, label paper 8 on reagent bottle 6 and the reagent pipe 7 sets up highly all to be greater than kit body 1 thickness, reagent bottle 6 sets up to the polyvinyl chloride material, reagent pipe 7 sets up to the polypropylene material, the quantity of reagent pipe 7 sets up to a plurality ofly, and is a plurality of reagent pipe 7 includes transparent and brown two kinds.
As shown in fig. 1 to 4, the embodiment specifically is as follows: when a user can store the reagent in the environment of 4 ℃, the culture plate 5 is placed at the position of the first storage tank 2, the second storage tank 3 can store a large square reagent bottle 6 which is convenient for storing the reagent with large use amount and needing to be taken for many times, a plurality of fixing grooves 4 are used for storing a plurality of reagent tubes 7 which are used for subpackaging and storing the reagent needing to be used for many times or not suitable for repeated freeze thawing, the stability is kept, meanwhile, the height of the reagent box body 1 is not more than the position of label paper 8 on the reagent bottle 6 and the reagent tube 7, the observation and fetching cannot be influenced, the reagent bottle 6 is transparent and convenient to observe, part of the reagent tubes 7 are brown, the light-resistant storage of part of easily decomposed solution is convenient, when the user can store the reagent in the environment of-20 ℃, the reagent can be contained in an aluminum plastic bag, the reagent with strict light-resistant requirement is sealed by the brown reagent tubes 7 and the aluminum plastic bag, the device integrates three-dimensional culture of tumor cell microspheres, a culture plate 5 and reagents required by detection of cell growth rate and mobility after drug action are used for directly completing the high-flux three-dimensional culture of the tumor cell microspheres and drug screening, the reagent tube 7 is arranged for subpackaging and storing the reagents which need to be used repeatedly and are not suitable for repeated freeze thawing, one tube is used at a time, the reagent failure caused by repeated freeze thawing and repeated freeze thawing is avoided, a U-shaped plate method is adopted and matched with a high-content three-dimensional tumor cell real-time imaging analysis instrument, the aim of cell balling is achieved by using a centrifugal method, the tumor microspheres are generated stably, uniformly and controllably with high flux, the in-vitro three-dimensional culture is realized, and the detection of important indexes such as tumor growth, metastasis and the like is carried out.
A connecting cover 9 is arranged on the outer side of the top end of the reagent tube 7, an observation window 10 is arranged in the middle of the connecting cover 9, a sealing gasket 11 is arranged on the inner wall of the connecting cover 9, the back adhesive of the label paper 8 is low-temperature-resistant back adhesive, the label paper 8 is printed by a carbon ribbon, the observation window 10 and the connecting cover 9 are integrally formed, the observation window 10 and the label paper 8 are arranged in a position corresponding to each other, and the connecting cover 9 is subjected to frosting treatment;
as shown in fig. 5 to 6, the embodiment is specifically as follows: the person of facilitating the use of setting up of label paper 8 numbers a plurality of reagent pipes 7, the mark, convenient differentiation, and low temperature gum and label paper 8 adopt the design of carbon ribbon printing mode, can guarantee that kit body 1 can be preserved at low temperature, label paper 8 can not appear dropping, will connect cover 9 covers in the reagent pipe 7 outside, sealed 11 multiplicable sealed sealings between cover 9 and the reagent pipe 7 of connecting, it is fuzzy to avoid the liquid outflow to cause the typeface on the label paper 8, the user can be observed the typeface on label paper 8 by observation window 10, avoid taking place the chaotic condition of sample and take place.
The utility model discloses the theory of operation:
referring to the attached figures 1-4 of the specification, when a user can respectively store reagents by using a culture plate 5 and an aluminum plastic bag at the temperature of 4 ℃ and the temperature of-20 ℃, a second storage tank 3 can store a square large reagent bottle 6, a plurality of fixing grooves 4 are used for storing a plurality of reagent tubes 7, a U-shaped plate method is adopted, a high-content three-dimensional tumor cell real-time imaging analyzer is matched, and the aim of cell balling is achieved by using a centrifugation method;
furthermore, referring to the drawings of the specification and fig. 5-6, the connecting cover 9 is sleeved outside the reagent tube 7, the sealing gasket 11 can increase the sealing degree between the connecting cover 9 and the reagent tube 7, the situation that the characters on the label paper 8 are fuzzy due to outflow of liquid is avoided, and a user can observe the characters on the label paper 8 through the observation window 10, so that the situation of sample confusion is avoided.
The points to be finally explained are: first, in the description of the present application, it should be noted that, unless otherwise specified and limited, the terms "mounted," "connected," and "connected" should be understood broadly, and may be a mechanical connection or an electrical connection, or a communication between two elements, and may be a direct connection, and "upper," "lower," "left," and "right" are only used to indicate a relative positional relationship, and when the absolute position of the object to be described is changed, the relative positional relationship may be changed;
secondly, the method comprises the following steps: in the drawings of the disclosed embodiments of the present invention, only the structures related to the disclosed embodiments are referred to, and other structures can refer to the common design, and under the condition of no conflict, the same embodiment and different embodiments of the present invention can be combined with each other;
and finally: the above description is only for the preferred embodiment of the present invention and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The utility model provides a three-dimensional culture of high flux tumour cell and drug screening kit, includes kit body (1), its characterized in that: the reagent kit body (1) top is equipped with first storage tank (2), first storage tank (2) one side is equipped with second storage tank (3), first storage tank (2) one end is equipped with fixed slot (4), first storage tank (2) inside is equipped with culture plate (5), second storage tank (3) inside is equipped with reagent bottle (6), fixed slot (4) inside is equipped with reagent pipe (7), reagent bottle (6) and reagent pipe (7) outer wall all are equipped with label paper (8), still be equipped with the plastic-aluminum bag in fixed slot (4), reagent pipe (7) top outside is equipped with connecting cover (9), connecting cover (9) middle part is equipped with observation window (10), connecting cover (9) inner wall is equipped with sealed pad (11).
2. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the number of the fixing grooves (4) is set to be a plurality of, and the fixing grooves (4) and the second storage grooves (3) longitudinally penetrate through the kit body (1).
3. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the aluminum plastic bag and the reagent tube (7) are matched with the fixing groove (4).
4. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the kit body (1) is made of sponge materials, the kit body (1) and the aluminum plastic bag are externally provided with packing boxes, and the packing boxes are packaged in an airplane box type.
5. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the label paper (8) on the reagent bottle (6) and the reagent tube (7) are arranged with heights larger than the thickness of the reagent box body (1).
6. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the reagent bottle (6) is made of polyvinyl chloride, and the reagent tube (7) is made of polypropylene.
7. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the number of the reagent tubes (7) is set to be a plurality, and the plurality of the reagent tubes (7) comprise both transparent and brown.
8. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: the back adhesive of the label paper (8) is low-temperature-resistant back adhesive, and the label paper (8) is printed by a carbon ribbon.
9. The kit for three-dimensional culture and drug screening of high-throughput tumor cells according to claim 1, wherein: observation window (10) and connecting cover (9) integrated into one piece, observation window (10) and label paper (8) position correspond the setting, connecting cover (9) do dull polish and handle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921001080.7U CN210437658U (en) | 2019-06-29 | 2019-06-29 | High-flux tumor cell three-dimensional culture and drug screening kit |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921001080.7U CN210437658U (en) | 2019-06-29 | 2019-06-29 | High-flux tumor cell three-dimensional culture and drug screening kit |
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| CN210437658U true CN210437658U (en) | 2020-05-01 |
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| CN201921001080.7U Active CN210437658U (en) | 2019-06-29 | 2019-06-29 | High-flux tumor cell three-dimensional culture and drug screening kit |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940917A (en) * | 2021-04-21 | 2021-06-11 | 济南创泽生物医药科技有限公司 | Automatic change cell culture equipment |
| CN113189071A (en) * | 2021-04-29 | 2021-07-30 | 上海交通大学 | Kit and imaging method for accurate imaging of three-dimensional network of blood vessel of complete organ |
-
2019
- 2019-06-29 CN CN201921001080.7U patent/CN210437658U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112940917A (en) * | 2021-04-21 | 2021-06-11 | 济南创泽生物医药科技有限公司 | Automatic change cell culture equipment |
| CN113189071A (en) * | 2021-04-29 | 2021-07-30 | 上海交通大学 | Kit and imaging method for accurate imaging of three-dimensional network of blood vessel of complete organ |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220121 Address after: 215000 floor 7, building 1, No. 188, Fuchunjiang Road, high tech Zone, Suzhou, Jiangsu Province Patentee after: Jiangsu Aiweide Biotechnology Co.,Ltd. Address before: 215163 floor 3, building 1, No. 8, Jinfeng Road, Suzhou Medical Device Industrial Park, high tech Zone, Suzhou, Jiangsu Province Patentee before: SOUTHEAST UNIVERSITY SUZHOU MEDICAL DEVICE Research Institute |
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| TR01 | Transfer of patent right |