CN217052187U - Box is stored in external amplification of cell - Google Patents

Abstract

The utility model discloses a box is stored in external amplification of cell, include the box body and put the pipe support, put the pipe support one end with the box body is connected, and the other end is provided with puts tub portion, it is used for placing the moisturizing pipe to put tub portion. Through set up on storing the box and put the pipe support, at the fluid infusion in-process, each cell culture sample uses the supplementary fluid infusion of putting the pipe support on storing the box separately, has avoided because of the sample sharing of a plurality of different sources puts the pipe support and causes sample cross contamination easily.

Description

Box is stored in external amplification of cell

Technical Field

The utility model relates to the field of biotechnology, concretely relates to box is stored in external amplification of cell.

Background

In vitro studies have shown that immune cells such as CIK, NK, etc. kill tumor cells primarily through a non-specific pattern, and therefore a large number of immune cells are usually required to see a significant killing effect, which requires large scale proliferation of immune cells in vitro. Adoptive cellular immunotherapy is a technology which collects autoimmune cells to activate and expand in vitro, and then re-infuses the autoimmune cells into a tumor patient body to promote the tumor cells to be killed in vivo, wherein the types, the quantity and the activity of effector cells are the key factors directly influencing the clinical curative effect.

The immune cells need to use a culture bottle and a culture bag in the culture process, and the culture bottle has small volume, poor air permeability, complex operation and easy pollution; the culture bag is made of air-permeable materials, has the characteristics of softness, transparency and good tightness, and is widely used for the later-stage mass amplification of immune cells. However, in the process of using the cell culture bag, multiple times of fluid infusion, sampling or cell observation under a microscope are needed, and the flexible cell culture bag is lack of support in the process of transportation and is easy to be punctured by a sharp instrument, so that fluid permeation is caused, and cell culture fails. In the process of fluid infusion, a plurality of specimens share one tube placing rack to easily cause specimen cross contamination.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a first purpose aims at providing a box is stored in external amplification of cell, avoids a plurality of cell amplification specimens at fluid infusion in-process cross contamination. The utility model discloses realize by following technical scheme:

the utility model provides a box is stored in external amplification of cell, includes the box body, its characterized in that: the liquid replenishing box further comprises a pipe placing frame, one end of the pipe placing frame is connected with the box body, the other end of the pipe placing frame is provided with a pipe placing part, and the pipe placing part is used for placing a liquid replenishing pipe; the inner wall of the box body is provided with a semicircular frame which is used for supporting a sampling port of the cell culture bag when the cell bag is placed in the box.

Among the above-mentioned technical scheme, through set up on storing the box and put the pipe support, at the fluid infusion in-process, each cell culture sample uses the supplementary fluid infusion of putting the pipe support of storing on the box separately, has avoided because of the sample sharing one of a plurality of different sources puts the pipe support and causes sample cross contamination easily.

As a further improvement of the utility model, the length of the pipe placing frame is the same as the length of the box body.

As a further improvement of the utility model, the pipe placing frame is provided with one end of the pipe placing part in a controlled way to contact or separate from the top of the side wall of the box body.

As a further improvement, the utility model discloses a lid still includes, lid detachably installs or connects on the box body, cover the opening of box body, put the pipe support one end with lid/box body are connected, be provided with on the lid/box body and put the pipe support and berth the position, put the pipe support controlled around with the one end that lid/box body are connected is rotated and is close to or keeps away from put the pipe support and berth the position.

As a further improvement, the pipe rack is placed and the stopping position is a pipe rack groove, the pipe rack is placed and controlled to enter or leave the pipe rack groove.

As a further improvement of the utility model, the box body is provided with an air vent, the air vent set up in the side of box body.

As a further improvement of the utility model, the collecting rack is further included, the collecting rack is arranged at one end of the box body, and the box body is controlled to protrude or be parallel and level or indent in the bottom surface of the box body.

As a further improvement of the utility model, an observation window is arranged on the bottom surface of the box body, and the cell shape inside the box body can be observed through the observation window.

As a further improvement of the utility model, a label pasting position is arranged on the box body for pasting the sample label.

In the utility model, the storage box is provided with the independent tube placing frame, thereby avoiding the cross contamination of the cell sample in the fluid infusion process; the incubator is convenient to store in a limited space of the incubator, and the space of the incubator is saved; and when the cell is collected, the cell culture bag does not need to be carried by an operator by hand, so that the work of the operator is facilitated.

Drawings

Fig. 1 is a perspective view of a collection frame of a cell in-vitro amplification storage box according to an embodiment of the present invention.

Fig. 2 is an enlarged view of a portion a in fig. 1.

Fig. 3 is a perspective view illustrating a collection frame of the in vitro cell expansion storage box according to an embodiment of the present invention retracted.

Fig. 4 is an enlarged view of a portion B in fig. 3.

Fig. 5 is a perspective view of a cell in-vitro amplification storage box provided in the second embodiment of the present invention.

Detailed Description

The following description will further describe embodiments of the present invention with reference to the accompanying drawings, wherein for convenience of description, the upper, lower, left, right, front, rear, and other orientations may be defined in the present application, and the present application is intended to clearly describe the relative position relationship of the structures, and is not intended to limit the actual orientation of the product during the manufacturing, using, selling, and the like. The following description of the embodiments of the present invention will be made with reference to the accompanying drawings:

example one

Referring to fig. 1-4, the present embodiment provides a storage box for in vitro cell amplification, which includes a box body 1, a tube placing rack 3 and a collecting rack 4.

Box body 1 is made by the organic glass material, and long 430mm, wide 270mm, high 30mm on two sides of box body 1, be provided with ten diameters and be 10 mm's air vent 11 for gaseous circulation does benefit to the culture bag and carries out gas exchange at the cell culture in-process, is favorable to the cell growth to breed. An observation window 12 is arranged on the bottom surface of the box body 1, and the cell growth and propagation conditions inside the box body 1 can be observed through the observation window 12. Collect frame 4 and set up on one side of box body 1, collect frame 4 and be extending structure, can bulge under manual control when using the bottom surface of box body 1, parallel and level or indent in when not using the bottom surface of box body 1. The inner bottom surface of the box body 1 is also provided with a semicircular frame 13, and when the semicircular frame 13 is used for cell sampling, a sampling port of a cell culture bag leans against the semicircular frame 13, so that the cell sampling is convenient. A label sticking position 14 is further provided on one side surface of the box body 1 for sticking a sample label (as another realizable technical solution, the semicircular frame 13 may also be provided on the inner wall of the box body 1 in other directions; and the label sticking position 14 may also be provided on the box cover 2).

Put 3 long 430mm of pipe support, wide 60mm, put the one end of pipe support 3 with the lateral wall top of box body 1 is connected, and the other end is provided with puts pipe portion 31, it is used for placing the fluid infusion pipe to put pipe portion 31 for place the syringe when replenishing the culture medium. Put pipe support 3 can be controlled around with the one end that box body 1 is connected rotates, is provided with put the one end of pipe portion 31 controlled with the lateral wall contact or the separation of box body, work as put the pipe support with during the lateral wall contact of box body, put 31 one end of pipe portion and put in on the lateral wall of box body, act as the lid of box body

When the cell in-vitro amplification storage box is used, a cell culture bag filled with cell suspension is placed in the storage box. The storage box is made of organic glass materials, is light in weight, firm, corrosion-resistant and easy to observe the cell proliferation condition inside the storage box. The storage boxes can be placed in the incubator in an overlapped mode, and the using space in the incubator is saved. During the culture process, when the condition of cell proliferation and culture solution needs to be observed, the storage box is taken out from the culture box, placed under a microscope, and the cell shape in the bag is observed through the observation window 12. When fluid infusion is needed, the storage box is placed in a superclean workbench, before fluid infusion, the pipe placing frame 3 is lifted to a proper height, the injector is placed on the pipe placing part 31 of the pipe placing frame 3, the fluid infusion port of the culture bag is connected with the injector, and fluid infusion operation is carried out on the culture bag; after the liquid is supplemented, pack up and put pipe support 3, make its put pipe portion 31 with the lateral wall contact of box body 1, the frame is in on the lateral wall of box body 1 and act as the lid of box body. During the cell culture, if the cells are to be sampled, the position of the sampling port of the culture bag is adjusted and leaned against the semicircular frame 13, and a pipette is used for sampling. After the culture is finished, the cells are collected, and the collecting frame 4 extends out to protrude out of the bottom surface of the box body 1, so that the storage box is inclined, and the cell bags are inclined in the storage box, thereby facilitating the outflow of cell suspension. The label pasting position 13 is used for pasting a sample label, and information such as sample processing time, reagent name, fluid infusion amount and the like is recorded on the sample label, so that memorandum and source tracing are facilitated.

Example two

Referring to fig. 5, the present embodiment provides a storage box for in vitro cell expansion, which further includes a box cover 2 based on the storage box for in vitro cell expansion provided in the first embodiment.

The box cover 2 is made of organic glass materials, is connected to the box body 1, and partially or completely covers the opening part of the box body 1. A pipe rack placing groove 21 (a pipe rack placing parking position) is concavely arranged on the box cover 2 towards the interior of the box body 1. The length and the width of the pipe placing frame 3 are set according to requirements, the pipe placing frame 3 can be controlled to rotate around one end connected with the box cover 2 and enter or leave the pipe placing frame groove 21, when the pipe placing frame 3 enters the pipe placing frame groove 21, the top surfaces of the pipe placing frame 3 and the box cover 2 are flush with or are inwards concave to the top surface of the box cover 2 (as other realizable technical schemes, the box cover 2 can also be detachably arranged on the box body 1, and the pipe placing frame 3 and the pipe placing frame groove 21 can be arranged on the box body 1).

When the storage box for in vitro cell expansion is used, a cell culture bag filled with cell suspension is placed in the storage box, and the box cover 2 is covered. When fluid infusion is needed, the storage box is placed in a super-clean workbench, before fluid infusion, the pipe placing frame 3 is lifted to be separated from the box cover from the pipe placing frame groove 21 and lifted to a proper height, the injector is placed on the pipe placing part 31 of the pipe placing frame 3, a fluid infusion port of the culture bag is connected with the injector, and fluid infusion operation is carried out on the culture bag (when the box cover 2 completely covers the opening part of the box body 1, the box cover 2 is also required to be opened); after the liquid supplementing is finished, the pipe placing frame 3 is folded and enters the pipe placing frame groove 21, and the liquid is convenient to store.

The above embodiments are merely for full disclosure and not for limitation, and all changes that can be made without inventive step based on the inventive idea of the present invention and equivalent technical features can be obtained without any inventive step should be considered as the scope of the present disclosure.

Claims (9)

1. The utility model provides a box is stored in external amplification of cell, includes the box body, its characterized in that: the liquid replenishing box further comprises a pipe placing frame, one end of the pipe placing frame is connected with the box body, the other end of the pipe placing frame is provided with a pipe placing part, and the pipe placing part is used for placing a liquid replenishing pipe; the inner wall of the box body is provided with a semicircular frame which is used for supporting a sampling port of the cell culture bag when the cell bag is placed in the box.

2. A cartridge according to claim 1, wherein: the length of the pipe placing frame is the same as that of the box body.

3. A cartridge according to claim 2, wherein: the pipe placing frame is provided with one end of a pipe placing part which is in contact with or separated from the top of the side wall of the box body in a controlled manner.

4. A cartridge according to claim 1, wherein: still include the lid, lid detachably installs or connects on the box body, cover the opening of box body, put the pipe support one end with lid/box body are connected, be provided with on the lid/box body and put the pipe support and berth the position, put the pipe support controlled around with the one end rotation that lid/box body are connected is close to or keeps away from put the pipe support and berth the position.

5. A cartridge according to claim 4, wherein: the parking position of the pipe rack placing device is a pipe rack placing groove, and the pipe rack placing device is controlled to enter or leave the pipe rack placing groove.

6. A cartridge according to claim 1, wherein: the box body is provided with an air vent which is arranged on the side surface of the box body.

7. A cartridge according to claim 1, wherein: still include and collect the frame, it sets up to collect the frame the one end of box body is controlled down protrusion or parallel and level or indent in the bottom surface of box body.

8. A cartridge according to claim 1, wherein: the bottom surface of the box body is provided with an observation window, and the cell shape in the box body can be observed through the observation window.

9. A cartridge according to any one of claims 1 to 8, wherein: the box body is provided with a label pasting position for pasting the sample label.

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