CN105505740B - A kind of PCR is loaded plate - Google Patents
A kind of PCR is loaded plate Download PDFInfo
- Publication number
- CN105505740B CN105505740B CN201510968725.4A CN201510968725A CN105505740B CN 105505740 B CN105505740 B CN 105505740B CN 201510968725 A CN201510968725 A CN 201510968725A CN 105505740 B CN105505740 B CN 105505740B
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- pcr
- base
- damping hinge
- shield
- console
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5085—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates
- B01L3/50851—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above for multiple samples, e.g. microtitration plates specially adapted for heating or cooling samples
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Devices For Use In Laboratory Experiments (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of PCR to be loaded plate, plinth including inner hollow, Damping hinge, shield and console, Damping hinge and console are separately positioned on two sidepieces of base, and console is detachably connected with base, shield is fixedly connected with the top of Damping hinge, cold-storage ice bag can be placed in the cavity of the base, and multiple loading slots are distributed with the upper surface of base, PCR sample-adding pipes can be placed in loading slot, the tip height of the PCR sample-addings pipe is identical with the tip height of Damping hinge, the shield can rotate around Damping hinge to be covered on PCR sample-adding pipes；The present invention can to keep low temperature level when PCR is loaded, can be stablized base temperature at 5 DEG C or so by hollow base and agent for storage of coldness ice bag, and not have to base being directly placed on ice；The present invention can be reduced because the data error that evaporation and environmental pollution are brought, and greatly reduce the influence of evaporation and pollution.
Description
Technical field
The present invention relates to life science and preclinical medicine field, specifically a kind of PCR sample-addings plate.
Background technology
PCR experiment is that the basic subject such as current biomolecule field and criminal investigation etc. are most universal, most common amplification purpose
DNA experimental method.PCR is needed to mix sample before the experiment, and these samples include amplification template, and forward direction is drawn
A variety of reagents such as thing, reverse primer, Taq enzyme, dNTP, Mg ions, deionized water.As technological progress is now by Taq
Enzyme is integrated, and Taq enzyme, dNTP, Mg ion etc. are become into premix system, is needed for experiment every time to hundreds of reagent
Mixed, it is such to change the operating efficiency for having greatly improved scientific research personnel.But some unconventional experiments, because
The limitation of experimental method still uses mixing of the aging method through row reagent sample.Simplify anyway, it is amplification template, forward direction, anti-
It is to be added separately to primer, deionized water.And for the system of Taq enzyme for not being premix, dNTP points are 4 kinds, Mg
The buffer solutions such as ion are also required to separately sequentially add.So minimum of one system needs points for 5 times for PCR, sequentially add
Different reagents, and most at most need more than 10 times.For scientific research operator, each system needs 5 times at most more than 10 at least
Secondary pipetting is very cumbersome time-consuming operation.Each pipetting only includes 1 μ L or so transparency liquid, is easy to out
Mistake, and in order to easy to operate, Cai the pipe of the more flux of general multiple rows of connecting leg and 96 orifice plates etc. is all on last before PCR machines
It can close the lid, which results in when largely processing laboratory sample, the part first handled and the part-time of post processing
On have a long way to go, such sample is exposed in air, first, sample itself is exactly micro, easily evaporation, causes in system
The change of portion's concentration.Second, if not in being operated in closed space, for sample easily by environmental pollution, these can all cause reality
Test the difference of result.
Because the relation of PCR instrument device, PCR pipe and more platoon pipes and 96 orifice plates are all fixed standards, and are consolidated
The sample-adding plate for determining PCR only has and single fixes this function.When that can not change the tubing of standard, this is with regard to active demand
A kind of PRC is loaded plate, and the well not operated or sample-adding pipe can be protected during prolonged operation sample-adding, and can
Ensure sample or be loaded the low temperature of pipe, can conveniently operate and effectively prevent to evaporate, the influence of environment.
The content of the invention
Easily evaporated for above-mentioned existing sample-adding plate and cause the change of system concentration inside, sample easily by environmental pollution
And the problems such as causing the difference of experimental result, the present invention provide a kind of PCR sample-addings plate.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of PCR is loaded plate, including the plinth of inner hollow, Damping hinge, shield and console, Damping hinge and
Console is separately positioned on two sidepieces of base, and console is detachably connected with base, the top of shield and Damping hinge
It is fixedly connected, cold-storage ice bag can be placed in the cavity of the base, and multiple loading slots, loading slot is distributed with the upper surface of base
Interior to place PCR sample-adding pipes, the tip height of the PCR sample-addings pipe is identical with the tip height of Damping hinge, and the shield can
Rotate and covered on PCR sample-adding pipes around Damping hinge；
Each sample-adding trench bottom is equipped with LED, and LED is connected by interface with the integrated circuit in console；
The base is connected with console by interface；
The base is made up of aluminium alloy；
The shield is made up of PP plastics；
The Damping hinge is made up of aluminium alloy；
The loading slot opening diameter is 5.1mm, and inside is conical downwards, depth 8mm；
The Damping hinge maximum folding angle is 75 °；
It is 12.5mm with the distance of base upper surface afterwards that the shield, which is laid flat, and louver width is 6.9 mm, length and base
Length is identical.
Beneficial effects of the present invention：
PCR provided by the invention is loaded plate, can to keep low temperature level when PCR is loaded, when environment temperature is 25 DEG C
When, can be stable at 5 DEG C or so by base temperature by hollow base and agent for storage of coldness ice bag, and without base is directly placed
On ice；The present invention can be reduced because evaporation and the data error brought of environmental pollution, when shield is put down can be with the mouth of pipe
It coincide, serves as the effect of lid, greatly reduces evaporation, and the influence of pollution；The operating desk of the present invention separates with base, can be with
Individually charging, what operating desk can control LED in base loading slot manually lights order, so greatly reduces leakage sample-adding product,
Add wrong sample cell, and repeat to add the error of reagent；The operating desk of the present invention separates with base, and base can be used alone,
The PCR sample-adding plate versions for being not added with LED can also be only produced when making or producing, can so be carried out at the sterilizing of HTHP
Reason；The present invention can ensure the low temperature of PCR samples, and the entrance of pollutant in environment is reduced in sample-adding, be equally applicable to
The sample-adding of single PCR pipe, the multi-joint combs of PCR, and 96 hole PCR versions uses.
Brief description of the drawings
Fig. 1 schematic structural views of the invention；
Reference：1st, base, 2, Damping hinge, 3, shield, 4, console.
Embodiment
With reference to embodiment, the present invention is further elaborated.
As shown in the figure：A kind of PCR is loaded plate, including the plinth 1 of inner hollow, Damping hinge 2, shield 3 and control
Platform 4, Damping hinge 2 and console 4 are separately positioned on two sidepieces of base 1, and console 4 is detachably connected with base 1, hide
Plate 3 is fixedly connected with the top of Damping hinge 2, and cold-storage ice bag, and the upper surface of base 1 can be placed in the cavity of the base 1
Multiple loading slots are distributed with, PCR sample-adding pipes, the tip height and Damping hinge 2 of the PCR sample-addings pipe can be placed in loading slot
Tip height is identical, and the shield 3 can rotate around Damping hinge 2 to be covered on PCR sample-adding pipes.
The making material of base 1 is aluminium alloy, and inner hollow can place cold-storage ice bag, and the Damping hinge 2 of side closes for aluminium
Gold, the shield 3 being connected with Damping hinge 2 are PP plastics.Horizontal stroke 12 vertical 8, are divided into 96 loading slots of cloth, loading slot includes on base 1
There is compact LED lamp holder, LED is connected by interface with the integrated circuit in console 4；Loading slot opening diameter is 5.1mm, interior
Portion is cone downwards, depth 8mm, can be coincide with the PCR pipe of standard, multi-joint comb, and 96 hole PCR plates, and damping is closed
The maximum folding angle of page 2 is 75 °, and when carrying out sample-adding operation using more platoon pipes and 96 orifice plates, LED can indicate currently
Loading sequence；It is 12.5mm with the distance of the upper surface of base 1 afterwards that the shield 3, which is laid flat, and louver width is 6.9 mm, length and bottom
1 length of seat is identical, and shield 3 can be put down after sample is added, and perfect can be bonded PCR pipe mouth, the work of the lid served temporarily as
With.Sample-adding plate base 1 is connected with operating desk 4 by interface, and rechargeable battery, and integrated operation button are included in operating desk 4,
LED can be controlled lights order, greatly reduces because being loaded string hole caused by carelessness or aggravating phenomenon.When operating desk 4 with
When base 1 separates, agent structure can still be independently operated, the auxiliary of simply no LED.
The part of base 1 can be put into the consistent cold-storage ice bag of size to be hollow, because main body is cast for aluminium alloy,
Can be stably by the temperature stabilization of sample cell at 5 DEG C or so.Damping hinge 2 connects shield 3, and the shield 3 is by PP plastics systems
Into the height of Damping hinge 2 is consistent with PCR pipe open height, ensures that shield can block the mouth of pipe when putting down.Operating desk 4 can be according to
Loading sequence, set LED lights order.
By taking 8 platoon pipes as an example, when PCR experiment operates progress, cold-storage ice bag is waited to reduce bulk temperature, then by 8
Platoon pipe is sequentially placed in sample cell, and the order of LED is set by console 4, then proceeds by PCR sample-adding behaviour
Make, completed for sample-adding or wait 8 platoon pipes of sample-adding just to put down corresponding shield, only retain the one group of shield operated
In opening.After the sample mixing in a hole is completed, the corresponding button of console is pressed, LED be able to will enter next
Lighted in the sample cell of row operation, and the LED for completing hole is then taken advantage of a situation extinguishing.After the completion of this 8 connecting leg sample mixing, screening is put down
Plate.By that analogy, until all sample cell mixing are completed.Likewise, for single PCR pipe and 96 orifice plates, this rule according to
So effectively.
Claims (9)
1. a kind of PCR is loaded plate, it is characterised in that：Plinth including inner hollow（1）, Damping hinge（2）, shield（3）
And console（4）, Damping hinge（2）And console（4）It is separately positioned on base（1）Two sidepieces, and console（4）The bottom of with
Seat（1）It is detachably connected, shield（3）With Damping hinge（2）Top be fixedly connected, the base（1）Cavity in place store
Freezing ice bag, and base（1）Upper surface multiple loading slots are distributed with, PCR sample-adding pipes, PCR sample-adding can be placed in loading slot
The tip height and Damping hinge of pipe（2）Tip height it is identical, the shield（3）Can be around Damping hinge（2）Rotation covers
On PCR sample-adding pipes.
2. PCR as claimed in claim 1 is loaded plate, it is characterised in that：Each sample-adding trench bottom is equipped with LED, LED
Lamp passes through interface and console（4）Interior integrated circuit connection.
3. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The base（1）With console（4）Pass through interface phase
Even.
4. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The base（1）It is made up of aluminium alloy.
5. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The shield（3）It is made up of PP plastics.
6. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The Damping hinge（2）It is made up of aluminium alloy.
7. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The loading slot opening diameter is 5.1mm, and inside is
Cone downwards, depth 8mm.
8. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The Damping hinge（2）Maximum folding angle is 75 °.
9. PCR as claimed in claim 1 is loaded plate, it is characterised in that：The shield（3）It is laid flat rear and base（1）Upper surface
Distance be 12.5mm, louver width is 6.9 mm, length and base（1）Length is identical.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510968725.4A CN105505740B (en) | 2015-12-22 | 2015-12-22 | A kind of PCR is loaded plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510968725.4A CN105505740B (en) | 2015-12-22 | 2015-12-22 | A kind of PCR is loaded plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105505740A CN105505740A (en) | 2016-04-20 |
| CN105505740B true CN105505740B (en) | 2017-12-15 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510968725.4A Active CN105505740B (en) | 2015-12-22 | 2015-12-22 | A kind of PCR is loaded plate |
Country Status (1)
| Country | Link |
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| CN (1) | CN105505740B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111423972B (en) * | 2020-05-05 | 2023-08-08 | 华中科技大学同济医学院附属协和医院 | PCR sample-adding monitoring device and monitoring method thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5306896A (en) * | 1992-06-22 | 1994-04-26 | Savant Instruments, Inc. | Method and apparatus for open digestion of specimens utilizing inclined container support means with electric heater |
| CN102375053A (en) * | 2010-08-20 | 2012-03-14 | 梁伟民 | Novel laboratory sample retention and preservation method |
| US9255936B2 (en) * | 2010-09-10 | 2016-02-09 | Hamilton Storage Technologies, Inc. | Sample storage cassette for ultra-low or cryogenic temperatures |
| CN102922735A (en) * | 2012-11-06 | 2013-02-13 | 无锡耐思生物科技有限公司 | Jig for adjusting deformation of 96-hole polymerase chain reaction (PCR) plate molded product with skirt edges |
| CN104560632A (en) * | 2014-12-12 | 2015-04-29 | 无锡南方制动件有限公司 | PCR (polymerase chain reaction) plate with neglected-loading viewing mirror cover |
| CN204569899U (en) * | 2015-04-09 | 2015-08-19 | 四川农业大学 | A kind of novel PC R experimental installation |
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- 2015-12-22 CN CN201510968725.4A patent/CN105505740B/en active Active
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| Publication number | Publication date |
|---|---|
| CN105505740A (en) | 2016-04-20 |
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Effective date of registration: 20190412 Address after: 471000 Luoyang City, Henan Province, China (Henan) Free Trade Pilot Zone Luoyang District Jianxi District Penglai Road 2 Luoyang National University Science Park 10 101 Patentee after: Luoyang Ono Biotechnology Co.,Ltd. Address before: Room 604, Building 3-1, Science Park, Luoyang National University, Longyu Road, Jianxi District, Luoyang City, Henan Province, 471000 Patentee before: HENAN NOVEL TISSUE ENGINEERING Co.,Ltd. |
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Effective date of registration: 20200716 Address after: 518000 Guangdong city of Shenzhen province Qianhai Shenzhen Hong Kong cooperation zone before Bay Road No. 1 building 201 room A (located in Shenzhen Qianhai business secretary Co. Ltd.) Patentee after: Shenzhen Fengfeng Biotechnology Co.,Ltd. Address before: 471000 Luoyang City, Henan Province, China (Henan) Free Trade Pilot Zone Luoyang District Jianxi District Penglai Road 2 Luoyang National University Science Park 10 101 Patentee before: Luoyang Ono Biotechnology Co.,Ltd. |
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Effective date of registration: 20230406 Address after: 471000 Luoyang City, Henan Province, China (Henan) Free Trade Pilot Zone Luoyang District Jianxi District Penglai Road 2 Luoyang National University Science Park 10 101 Patentee after: New Hanfang (Henan) Life Technology Co.,Ltd. Address before: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.) Patentee before: Shenzhen Fengfeng Biotechnology Co.,Ltd. |
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