CN216051765U - Detection reagent strip for circulating tumor cells - Google Patents
Detection reagent strip for circulating tumor cells Download PDFInfo
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- CN216051765U CN216051765U CN202121694848.0U CN202121694848U CN216051765U CN 216051765 U CN216051765 U CN 216051765U CN 202121694848 U CN202121694848 U CN 202121694848U CN 216051765 U CN216051765 U CN 216051765U
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 53
- 238000001514 detection method Methods 0.000 title claims abstract description 49
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 107
- 238000004043 dyeing Methods 0.000 claims abstract description 30
- 238000007789 sealing Methods 0.000 claims abstract description 25
- 239000000243 solution Substances 0.000 claims abstract description 24
- 239000012192 staining solution Substances 0.000 claims abstract description 21
- 239000002699 waste material Substances 0.000 claims abstract description 13
- 229920004890 Triton X-100 Polymers 0.000 claims abstract description 4
- 239000013504 Triton X-100 Substances 0.000 claims abstract description 4
- 229920006324 polyoxymethylene Polymers 0.000 claims abstract description 4
- 238000004140 cleaning Methods 0.000 claims description 19
- 239000008191 permeabilizing agent Substances 0.000 claims description 18
- 238000007689 inspection Methods 0.000 claims description 7
- 230000000007 visual effect Effects 0.000 claims description 2
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 239000012141 concentrate Substances 0.000 abstract 1
- 238000010186 staining Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 3
- 238000003745 diagnosis Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000007850 fluorescent dye Substances 0.000 description 3
- 238000001215 fluorescent labelling Methods 0.000 description 3
- 230000008823 permeabilization Effects 0.000 description 3
- 210000003813 thumb Anatomy 0.000 description 2
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 101000738771 Homo sapiens Receptor-type tyrosine-protein phosphatase C Proteins 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102100037422 Receptor-type tyrosine-protein phosphatase C Human genes 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000002331 protein detection Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000012128 staining reagent Substances 0.000 description 1
- 210000004881 tumor cell Anatomy 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a detection reagent strip for circulating tumor cells, which comprises a body, wherein the body is provided with: the detection hole is used for inserting the microfluidic chip clamp and storing sample waste liquid and reagent waste liquid flowing through the microfluidic chip in the microfluidic chip clamp; a stationary liquid port in which polyformaldehyde is stored; a permeabilizer well having Triton X-100 disposed therein; a wash solution well in which PBS is stored; a sealing liquid hole in which a sealing liquid is stored; a first staining solution well in which a first fluorescently labeled antibody is stored; and a second staining solution well in which a second fluorescently labeled antibody is stored; wherein the first fluorescently labeled antibody and the second fluorescently labeled antibody are different. This detect reagent strip can concentrate multiple dyeing liquid and reagent and deposit in a reagent strip, and the convenience of taking avoids polluting, and the simplified operation can reduce the cost that the single detected.
Description
Technical Field
The utility model belongs to the technical field of biological detection, and relates to a detection reagent strip for circulating tumor cells.
Background
Circulating Tumor Cells (CTCs) are one of the key detection indicators for in vitro diagnosis of tumors. In the field of biological analysis, through the specific combination of cells and antibodies, the directional enrichment and capture of circulating tumor cells with different protein phenotypes can be realized. After the enrichment is completed, the collected circulating tumor cells are subjected to label detection, so that the identification of the tumor cells can be realized. In clinical diagnosis, the detection of circulating tumor cells can effectively provide diagnosis basis and medication guidance, save medical cost and accelerate diagnosis and treatment process. At present, reagents are usually separately packaged by containers with different specifications, so that the reagents are inconvenient to take and easy to topple over, and pollution is caused; meanwhile, the detection process has the problems of complex operation, high single detection cost and the like.
SUMMERY OF THE UTILITY MODEL
Based on the existing problems, the utility model aims to provide a reagent strip for detecting circulating tumor cells, which is convenient to take, avoids pollution, simplifies operation and can reduce the cost of single detection by intensively storing various staining solutions and reagents in one reagent strip.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a detection reagent strip for circulating tumor cells comprises a body, wherein the body is provided with:
the detection hole is used for inserting the microfluidic chip clamp and storing sample waste liquid and reagent waste liquid flowing through the microfluidic chip in the microfluidic chip clamp;
a stationary liquid port in which polyformaldehyde is stored;
a permeabilizer well having Triton X-100 disposed therein;
a wash solution well in which PBS is stored;
a sealing liquid hole in which a sealing liquid is stored;
a first staining solution well in which a first fluorescently labeled antibody is stored; and
a second staining solution well in which a second fluorescently labeled antibody is deposited;
wherein the first fluorescently labeled antibody and the second fluorescently labeled antibody are different.
Preferably, the detection hole, the fixing liquid hole, the permeabilizing agent hole, the cleaning liquid hole, the first staining liquid hole and the second staining liquid hole extend downward from the upper surface of the body respectively.
Preferably, the fixing liquid hole, the permeabilizing agent hole, the first dyeing liquid hole, the second dyeing liquid hole and the sealing liquid hole are step holes respectively, each step hole comprises an upper hole portion and a lower hole portion, and the hole diameter of the upper hole portion is larger than that of the lower hole portion in a top view. The design of the stepped hole can be matched with the sealing element, so that the sealing performance of the detection reagent strip is ensured.
Preferably, in a top view, the upper hole portion is a square hole, the lower hole portion is a circular hole, and a depth of the upper hole portion is less than one tenth of a depth of the lower hole portion.
Preferably, the body is further provided with a positioning hole for positioning the detection reagent strip.
Preferably, the body is further provided with a groove for facilitating holding of a user.
Preferably, the body extends along left right direction, it is right still to have seted up the application of sample pipe on the body and moved back the position groove, be used for detection reagent strip carries out the locating hole of fixing a position and the recess that the person of facilitating the use gripped, the locating hole the application of sample pipe moves back the position groove wash the liquid hole with the inspection hole sets up along left right direction interval, fixed liquid hole with the permeabilizing agent hole set up in wash the liquid hole with just set up side by side along the fore-and-aft direction between the inspection hole, seal the liquid hole first dyeing liquid hole with second dyeing liquid hole set up in wash the liquid hole with the application of sample pipe moves back between the position groove.
Preferably, the positioning hole is a through hole through which light can pass.
Preferably, a cross section of the groove in the vertical direction has a downwardly convex arc shape or is a downwardly convex arc shape. Furthermore, in order to facilitate the taking and holding of a user, the cross section of the groove along the vertical direction is provided with an arc protruding downwards, and the arc can be attached to the shape of a thumb, so that the groove is not easy to drop or slide.
Preferably, the positioning hole is located at the left end of the body, and the groove is located at the right end of the body.
Preferably, the test reagent strip further comprises sealing members for respectively closing the holes on the body, so that reagent leakage can be prevented during transportation or taking.
The utility model also adopts the following technical scheme:
the utility model provides a detection reagent strip, includes the body, the inspection hole, fixed liquid hole, permeabilization agent hole, cleaning fluid hole, closed liquid hole, first dyeing liquid hole and second dyeing liquid hole have been seted up on the body, the inspection hole fixed liquid hole permeabilization agent hole cleaning fluid hole first dyeing liquid hole with second dyeing liquid hole is certainly respectively the upper surface downwardly extending of body, fixed liquid hole permeabilization agent hole first dyeing liquid hole second dyeing liquid hole with closed liquid hole is the step hole respectively, the step hole includes hole portion and lower hole portion, overlooking the visual angle, the aperture of last hole portion is greater than lower hole portion. The design of the stepped hole can be matched with the sealing element, so that the sealing performance of the detection reagent strip is ensured.
Compared with the prior art, the utility model has the following advantages by adopting the scheme:
the detection reagent strip for circulating tumor cells provided by the utility model can be used for intensively storing various reagents and various different staining solutions for detection in the same detection reagent strip, and additional reagent containers and storage means are not needed, so that the cost can be reduced, and the operation steps can be simplified; reagents are independently and separately stored, so that liquid mixing and leakage are avoided, and the risk of pollution can be reduced; the detection hole can be used for inserting the microfluidic chip clamp for detection, and can also be used for storing the waste liquid left after the detection is finished, so that the space of the detection reagent strip can be saved, and the problems of storage and treatment of the waste liquid do not need to be considered additionally.
Drawings
In order to more clearly illustrate the technical solution of the present invention, the drawings needed to be used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings based on the drawings without creative efforts.
FIG. 1 is a schematic diagram of a test strip for circulating tumor cells, according to an embodiment;
FIG. 2a is a top view of a test strip according to an embodiment;
FIG. 2b is a cross-sectional view A-A of FIG. 2 a;
FIG. 2c is a cross-sectional view B-B of FIG. 2 a;
FIG. 2d is a cross-sectional view C-C of FIG. 2 a;
fig. 2e is a cross-sectional view D-D in fig. 2 a.
Wherein,
1. a body; 2. a groove; 21. arc-shaped; 3. a detection hole; 4. a stationary liquid port; 5. permeabilizing agent pores; 6. cleaning fluid holes; 7. sealing the liquid hole; 8. a first staining solution well; 9. a second staining solution well; 11. a sample adding pipe withdrawal groove; 12. positioning holes;
10. an upper hole portion; 20. a lower hole portion.
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the advantages and features of the utility model may be more readily understood by those skilled in the art. It should be noted that the description of the embodiments is provided to help understanding of the present invention, but the present invention is not limited thereto.
As used in this specification and the appended claims, the terms "comprises" and "comprising" are intended to only encompass the explicitly identified steps and elements, which do not constitute an exclusive list, and that a method or apparatus may include other steps or elements. As used herein, the term "and/or" includes any combination of one or more of the associated listed items.
It should be noted that, unless otherwise specified, when a feature is referred to as being "fixed" or "connected" to another feature, it may be directly fixed or connected to the other feature or indirectly fixed or connected to the other feature. Further, the description of the upper, lower, left, right, etc. used in the present invention is only with respect to the positional relationship of the respective components of the present invention with respect to each other in the drawings.
It is further understood that the use of "a plurality" in this disclosure means two or more, as other terms are analogous.
As shown in FIG. 1 and FIG. 2a, the test strip for circulating tumor cells of this embodiment, the reagent strip comprises a body 1, wherein the body 1 extends along the left-right direction, a positioning hole 12, a sample adding pipe withdrawal groove 11, a cleaning liquid hole 6, a detection hole 3 and a groove 2 are arranged at intervals along the left-right direction, a fixing liquid hole 4 and a permeabilizing agent hole 5 are arranged between the cleaning liquid hole 6 and the detection hole 3 and are arranged in parallel along the front-back direction, a sealing liquid hole 7, a first dyeing liquid hole 8 and a second dyeing liquid hole 9 are arranged between the cleaning liquid hole 6 and the sample adding pipe withdrawal groove 11, wherein the closed liquid hole 7 and the first dyeing liquid hole 8 are disposed between the cleaning liquid hole 6 and the second dyeing liquid hole 9 and are arranged in parallel in the front-rear direction, specifically, the closed liquid hole 7 and the first dyeing liquid hole 8 are arranged in parallel in the front-rear direction, and the second dyeing liquid hole 9 and an empty hole are arranged in parallel in the front-rear direction. The positioning hole 12 is located at the left end of the body 1, and the groove 2 is located at the right end of the body 1.
The groove 2 is convenient for a user to hold, the detection hole 3 is used for allowing the microfluidic chip clamp to insert and store sample waste liquid and reagent waste liquid flowing through a microfluidic chip in the microfluidic chip clamp, polyformaldehyde is stored in the fixed liquid hole 4, Triton X-100 is stored in the permeabilizing agent hole 5, PBS (primary reagent) is stored in the cleaning liquid hole 6, and bovine serum albumin sealing liquid is stored in the sealing liquid hole 7; a first fluorescence labeling antibody is stored in the first staining solution hole 8, a second fluorescence labeling antibody is stored in the second staining solution hole 9, the sample adding tube withdrawal groove 11 is used for withdrawing the liquid adding TIP head, and the positioning hole 12 is used for positioning the detection reagent strip.
The detection reagent strip also comprises sealing elements for respectively closing the holes on the body 1, so that reagent leakage possibly generated in the process of transportation or taking can be prevented. The seal may in particular be a removable or pierceable sealing membrane.
As shown in fig. 2b, the cross section of the groove 2 in the vertical direction has a downwardly convex arc shape or is a downwardly convex arc shape. In this embodiment, in order to facilitate the user to take and hold, the cross section of the groove 2 in the vertical direction has a downwardly convex arc 21, which can be fitted to the shape of the thumb, so that it is not easy to fall or slip off. The sample adding pipe retreats from groove 11, inspection hole 3, fixed liquid hole 4, permeabilizing agent hole 5, cleaning solution hole 6, seals liquid hole 7, first staining solution hole 8 and second staining solution hole 9 and extends downwards from the upper surface of body 1 respectively. The sample adding pipe withdrawal groove 11, the fixing liquid hole 4, the permeabilizing agent hole 5, the first dyeing liquid hole 8, the second dyeing liquid hole 9 and the sealing liquid hole 7 are respectively stepped holes. The stepped hole includes an upper hole portion 10 and a lower hole portion 20, and the upper hole portion 10 has a larger hole diameter than the lower hole portion 20 in a plan view. As shown in fig. 2c, the upper hole portion 10 and the lower hole portion 20 of the cuvette withdrawal groove 11 are elliptical holes and are located inside the main body 1. In addition, as shown in fig. 2d and 2e, the upper hole portions 10 of the remaining holes are square holes, the lower hole portions 20 are circular holes, and the upper hole portions 10 are provided on the upper surface of the main body 1 and outside the holes. The height of the upper hole part 10 is less than one tenth of the depth of the lower hole part 20, and this is designed to match the shape of the above-mentioned hole with the shape of the micro-fluidic chip clamp, so that the micro-fluidic chip clamp can be inserted into each hole conveniently and can play a role in positioning and fixing when in use. The upper ends of the fixed liquid hole 4, the permeabilizing agent hole 5, the cleaning liquid hole 6, the closed liquid hole 7, the first dyeing liquid hole 8 and the second dyeing liquid hole 9 have a height difference with the upper surface of the body 1, specifically, the part of the body 1 surrounding each hole is higher than the part far away from the hole, so that the part near the hole slightly protrudes upwards, each hole can be sealed after the sealing film is covered on the body 1, and each hole can be better sealed. Preferably, the height difference between the upper ends of the fixing liquid hole 4, the permeabilizing agent hole 5, the cleaning liquid hole 6, the sealing liquid hole 7, the first staining liquid hole 8 and the second staining liquid hole 9 and the upper surface of the body 1 is 0.5mm-5mm, and the single-edge width of the upper ends of the fixing liquid hole 4, the permeabilizing agent hole 5, the cleaning liquid hole 6, the sealing liquid hole 7, the first staining liquid hole 8 and the second staining liquid hole 9 is 0.5mm-5 mm. The positioning hole 12 is vertically through the left side of the body 1 and allows light to pass through.
The first and second fluorescently labeled antibodies stored in the first and second staining solution wells 8 and 9 are different, for example, one is fluorescently labeled CD45 or pan-CK antibody, and the other is fluorescently labeled goat anti-rabbit antibody. In the embodiment, the adopted first fluorescence-labeled antibody displays green fluorescence, the second fluorescence-labeled antibody displays orange-red fluorescence, the two staining solutions are different in color and do not interfere with each other, the cross color phenomenon does not exist, and the detection result can be clearly and accurately displayed.
The detection reagent strip for circulating tumor cells is characterized in that a detection hole 3, a fixing liquid hole 4, a permeabilizing agent hole 5, a cleaning liquid hole 6, a sealing liquid hole 7, a first staining liquid hole 8 and a second staining liquid hole 9 are formed in a body 1, two different fluorescence labeling antibodies are respectively stored in the first staining liquid hole 8 and the second staining liquid hole 9, immunofluorescence identification of specific combination of the antibodies and circulating tumor cell surface protein markers is directly carried out, the specificity of protein detection is strong, and the detection result is clear; the reagent strip can be used for intensively storing various reagents and two different staining solutions for detection in the same detection reagent strip, and no additional reagent container or storage means is needed, so that the cost can be reduced; the reagents are independently and separately stored, so that liquid mixing and leakage are avoided, and the risk of pollution can be reduced; the body 1 is provided with a positioning hole for positioning the detection reagent strip and a groove for being held by a user, so that the use is convenient; the detection reagent strip can complete multiple steps of liquid transferring, sorting, waste liquid treatment and the like in the body 1, can complete enrichment and dyeing of circulating tumor cells without liquid transferring, and can simplify operation; the reagent is disposable, the detection hole 3 for detecting the reagent strip can be used for inserting the microfluidic chip clamp for detection, and can also be used for storing the residual waste liquid after the detection is finished, so that the space for detecting the reagent strip can be saved, and the problems of storage and treatment of the waste liquid do not need to be considered additionally.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and are preferred embodiments, which are intended to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the scope of the present invention. All equivalent changes or modifications made according to the principles of the present invention should be covered within the protection scope of the present invention.
Claims (10)
1. The utility model provides a detect reagent strip for circulating tumor cell, includes the body, its characterized in that, seted up on the body:
the detection hole is used for inserting the microfluidic chip clamp and storing sample waste liquid and reagent waste liquid flowing through the microfluidic chip in the microfluidic chip clamp;
a stationary liquid port in which polyformaldehyde is stored;
a permeabilizer well having Triton X-100 disposed therein;
a wash solution well in which PBS is stored;
a sealing liquid hole in which a sealing liquid is stored;
a first staining solution well in which a first fluorescently labeled antibody is stored; and
a second staining solution well in which a second fluorescently labeled antibody is deposited;
wherein the first fluorescently labeled antibody and the second fluorescently labeled antibody are different.
2. The test reagent strip of claim 1, wherein the detection well, the fixing solution well, the permeabilizing agent well, the washing solution well, the first staining solution well, and the second staining solution well each extend downward from an upper surface of the body.
3. The test reagent strip according to claim 1, wherein the fixing solution hole, the permeabilizing agent hole, the first staining solution hole, the second staining solution hole, and the blocking solution hole are stepped holes, respectively, and the stepped holes include an upper hole portion and a lower hole portion, and the upper hole portion has a larger hole diameter than the lower hole portion in a top view.
4. The strip of claim 3, wherein the upper aperture portion is a square aperture and the lower aperture portion is a circular aperture, and wherein the upper aperture portion has a depth less than one tenth of the depth of the lower aperture portion in a top view.
5. The test reagent strip of claim 1, wherein the body further defines a positioning hole for positioning the test reagent strip; and/or the body is also provided with a groove for being held by a user conveniently.
6. The strip according to claim 1, wherein the body extends in a left-right direction, the body further comprises a sample tube returning groove, a positioning hole for positioning the strip and a groove convenient for a user to hold, the positioning hole, the sample tube returning groove, the cleaning solution hole and the detection hole are arranged at intervals in the left-right direction, the fixing solution hole and the permeabilizing agent hole are arranged between the cleaning solution hole and the detection hole and arranged side by side in a front-back direction, and the sealing solution hole, the first dyeing solution hole and the second dyeing solution hole are arranged between the cleaning solution hole and the sample tube returning groove.
7. The strip according to claim 5 or 6, wherein the positioning hole is a through hole through which light can pass; and/or the section of the groove along the vertical direction has a downward convex arc shape or is a downward convex arc shape.
8. The test reagent strip of claim 6 wherein the positioning hole is located at a left end of the body and the recess is located at a right end of the body.
9. The test reagent strip of claim 1, further comprising a sealing member for closing the respective holes of the body.
10. The utility model provides a detection reagent strip, includes the body, its characterized in that, the inspection hole, fixed liquid hole, permeabilizing agent hole, cleaning solution hole, closed liquid hole, first dyeing solution hole and second dyeing solution hole have been seted up on the body, the inspection hole fixed liquid hole the permeabilizing agent hole the cleaning solution hole first dyeing solution hole with second dyeing solution hole is respectively certainly the upper surface downwardly extending of body, fixed liquid hole the permeabilizing agent hole first dyeing solution hole the second dyeing solution hole with closed liquid hole is the step hole respectively, the step hole includes upper aperture portion and lower aperture portion, overlook under the visual angle, the aperture of upper aperture portion is greater than lower aperture portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121694848.0U CN216051765U (en) | 2021-07-23 | 2021-07-23 | Detection reagent strip for circulating tumor cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121694848.0U CN216051765U (en) | 2021-07-23 | 2021-07-23 | Detection reagent strip for circulating tumor cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216051765U true CN216051765U (en) | 2022-03-15 |
Family
ID=80617994
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121694848.0U Active CN216051765U (en) | 2021-07-23 | 2021-07-23 | Detection reagent strip for circulating tumor cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216051765U (en) |
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2021
- 2021-07-23 CN CN202121694848.0U patent/CN216051765U/en active Active
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