CN111659477A - Independent sample introduction micro-fluidic immunoassay joint inspection device - Google Patents
Independent sample introduction micro-fluidic immunoassay joint inspection device Download PDFInfo
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- CN111659477A CN111659477A CN202010530477.6A CN202010530477A CN111659477A CN 111659477 A CN111659477 A CN 111659477A CN 202010530477 A CN202010530477 A CN 202010530477A CN 111659477 A CN111659477 A CN 111659477A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
Abstract
The invention provides an independent sample introduction microfluidic immunoassay joint inspection device which comprises a joint inspection strip, a washing supply unit and a waste liquid absorption and storage unit, wherein the head end of the joint inspection strip is connected with the washing supply unit, and the tail end of the joint inspection strip is connected with the waste liquid absorption and storage unit; the joint inspection strip is provided with a first micro-flow channel and a second micro-flow channel in a concave mode, a plurality of detection units are arranged between the first micro-flow channel and the second micro-flow channel on the joint inspection strip, each detection unit is a sample storage tank, a marker storage tank and an item reaction detection tank are arranged on the joint inspection strip in a concave mode, a plurality of markers corresponding to a plurality of targets are arranged in the marker storage tanks respectively, and the inner walls of the item reaction detection tanks are fixedly provided with solid phase components used for capturing the targets or the corresponding markers respectively. The device has high detection sensitivity and avoids mutual interference of a plurality of items.
Description
Technical Field
The invention relates to an independent sample injection microfluidic immunoassay joint inspection device, and belongs to the technical field of immunoassay.
Background
In the medical diagnosis process, a plurality of indexes are required to be jointly detected for the majority of disease diagnosis, and mutual evidence is adopted to assist the disease diagnosis, so that missed diagnosis or misdiagnosis is avoided, and the diagnosis accuracy is improved. Meanwhile, the multi-index combined detection is also used for clinically differential diagnosis of diseases, such as in the investigation and diagnosis of new coronavirus (nCOVI) epidemic induced new coronary pneumonia (covi-19), and for diagnosis of a patient or a suspected population, the patient should be informed of whether the patient is infected by the new coronavirus (nCOVI) or not, and if not, the patient should be informed of the disease state caused by other pathogens as much as possible, so that the multi-index combined detection is also required. Therefore, there is an important practical need to develop a real-time diagnosis of multi-index joint detection.
At present, the multi-index joint detection mostly adopts the labeled immune joint detection based on lateral chromatography, and the method generally has two detection methods. The first is to arrange different detection items in sequence and solid-phase on the same nitrocellulose solid-phase membrane (NC membrane), label objects of different items are layered together, when in detection, a sample permeates into a label object storage position from top to bottom and is combined with a corresponding labeled antigen or antibody, enters the NC membrane through a contact area with the NC membrane, moves to a solid-phase area by virtue of capillary action and is combined with a solid-phase component for two times and deposited to form a visible characteristic detection band, and the defect is that the different items possibly interfere with each other, especially the corresponding antigen and antibody of one molecule cannot be detected simultaneously, and the number of the detected labels is limited simultaneously. The second method is to prepare different items into independent joint inspection strips, then place the independent joint inspection strips in different card slots of the same plastic card respectively, and share a sample adding pool or add samples independently respectively, but the detection efficiency is low.
Disclosure of Invention
The invention provides an independent sample injection microfluidic immunoassay joint inspection device which can effectively solve the problems.
The invention is realized by the following steps:
an independent sample introduction microfluidic immunoassay joint inspection device comprises a joint inspection strip, a washing supply unit and a waste liquid absorption and storage unit, wherein the washing supply unit is connected to the head end of the joint inspection strip, and the waste liquid absorption and storage unit is connected to the tail end of the joint inspection strip;
the joint detection strip is concavely provided with a first micro-flow channel and a second micro-flow channel, the first micro-flow channel and the second micro-flow channel are arranged along the length direction of the joint detection strip, one end of the first micro-flow channel is communicated with the head end of the joint detection strip through an opening, the other end of the first micro-flow channel is sealed, one end of the second micro-flow channel is communicated with the tail end of the joint detection strip through an opening, and the other end of the second micro-flow channel is sealed; the head end of the joint inspection strip is provided with a membrane breaking prick, and the membrane breaking prick is communicated with the opening of the first micro-flow channel through a channel; a plurality of detection units are arranged on the joint detection strip between the first micro-flow channel and the second micro-flow channel, each detection unit is provided with a sample storage tank, a marker storage tank and an item reaction detection tank which are concavely arranged on the joint detection strip, sample inlets are arranged between the sample storage tanks and the marker storage tanks for communication, marker inlets are arranged between the marker storage tank and the item reaction detection tanks for communication, a plurality of washing solution inlets are arranged on the wall of the first micro-flow channel for communication with the plurality of sample storage tanks respectively, and a plurality of waste liquid outlets are arranged on the wall of the second micro-flow channel for communication with the plurality of item reaction detection tanks respectively; a film covering seal is arranged on the upper surface of the joint detection strip, so that a sealed communicating cavity channel is formed by the first micro-flow channel, the second micro-flow channel, the plurality of sample storage tanks, the plurality of marker storage tanks and the plurality of item reaction detection tanks; observation windows are arranged on the walls of the joint inspection strips corresponding to the bottoms or the side surfaces of the project reaction detection grooves; the plurality of label storage tanks are respectively filled with labels corresponding to the plurality of targets, and the inner walls of the plurality of item reaction detection tanks are respectively fixedly provided with solid phase components for capturing the plurality of targets or the corresponding labels;
the waste liquid absorbing and storing unit comprises a waste liquid sleeve, a negative pressure forming valve plate and a first sealing ring; the negative pressure forming valve plate is arranged in an inner cavity of the waste liquid sleeve, the first sealing ring seals an inlet end of the waste liquid sleeve and is provided with a first hollow matched with the shape of the cross section of the tail end of the joint inspection strip, a second hollow matched with the shape of the cross section of the tail end of the joint inspection strip is arranged in the center of the negative pressure forming valve plate, the tail end of the joint inspection strip extends into the waste liquid sleeve from the first hollow, the negative pressure forming valve plate is fixedly sleeved on the tail end of the joint inspection strip through the second hollow, and an opening of the second microflow channel is communicated with an inner cavity of an outlet end of the waste liquid sleeve; when the waste liquid sucking and storing unit slides towards the tail end far away from the joint detection strip, the negative pressure forming valve plate does not move, and negative pressure is generated in the inner cavity of the outlet end of the negative pressure forming valve plate and the waste liquid sleeve to suck the second microflow channel.
As a further improvement, the washing supply unit comprises a washing liquid sleeve, a washing liquid pipe and a second sealing ring; the washing liquid pipe is arranged in the washing liquid sleeve, the second sealing ring seals the inlet end of the washing liquid sleeve and is provided with a third hollow matched with the cross section shape of the head end of the joint detection strip, the head end of the joint detection strip extends into the washing liquid sleeve from the third hollow, the pipe opening of the washing liquid pipe is coated with a sealing film, and a film breaking prick at the head end of the joint detection strip is opposite to the sealing film; the washing liquid pipe is filled with washing liquid; when the joint inspection strip is pushed towards the washing supply unit, the membrane-breaking pricks the sealing membrane, and the cleaning liquid in the liquid washing pipe flows into the first microflow channel in the joint inspection strip from the channel of the membrane-breaking prick for washing.
In a further improvement, the first microfluidic channel, the second microfluidic channel, the sample reservoir, the label reservoir and the item reaction detection chamber are all surface-treated to be hydrophilic.
As a further improvement, the marker is selected from colloidal gold, colored microspheres, fluorescent substances, lanthanide rare earth element Eu2+And quantum dots.
As a further improvement, the joint inspection strip is integrally formed by compression molding or injection molding.
As a further improvement, a connecting rod extends out of the outer wall of a waste liquid sleeve of the waste liquid absorption and storage unit along the direction of the joint detection strip, a sliding groove is formed in the wall of a washing liquid sleeve of the washing and supply unit, and the extending end of the connecting rod is inserted into the sliding groove to clamp the washing and supply unit and the waste liquid absorption and storage unit together.
As a further improvement, the sample inlet is lower than the bottom surface of the sample storage tank.
As a further improvement, the waste liquid outlet is higher than the bottom surface of the item reaction detection tank.
As a further improvement, the number of the detection units is 3 to 10.
The invention also provides a use method of the independent sample injection microfluidic immunoassay joint inspection device, which comprises the following steps:
s1, adding sample liquid into each sample storage tank for reaction;
s2, sliding the waste liquid absorbing and storing unit to the sample outlet end far away from the joint inspection strip at the speed of 3-6 mm/S;
s3, after the reaction is finished, the joint inspection strip is pushed to the washing supply unit until a falling feeling is felt, and at the moment, a membrane rupture prick pierces the sealing membrane of the liquid washing tube to wash;
and S4, observing or calculating the detection result through visual inspection, colorimetric or fluorescent counting on the observation window of the joint inspection strip after the washing is finished.
The invention has the beneficial effects that:
the invention relates to an independent sample injection microfluidic immunoassay joint inspection device, wherein a first microfluidic channel and a second microfluidic channel are concavely arranged on a joint inspection strip, a plurality of detection units are arranged between the first microfluidic channel and the second microfluidic channel on the joint inspection strip, each detection unit is provided with a sample storage tank, a marker storage tank and an item reaction detection tank which are concavely arranged on the joint inspection strip, markers corresponding to a plurality of targets are respectively arranged in the marker storage tanks, and solid phase components for capturing the targets or the corresponding markers are respectively and fixedly arranged on the inner walls of the item reaction detection tanks. When the sample liquid is added into each sample storage tank respectively, under the capillary action, the sample liquid enters the marker storage tank and the project reaction detection tank in sequence, a target object in the sample liquid is combined with a corresponding marker and then combined with a corresponding solid phase in the project reaction detection tank, then the results are observed and recorded in a visual inspection mode, a colorimetric mode, a fluorescent mode and the like and reported qualitatively or quantitatively through the observation window, the solid phase and the marker are arranged at different positions respectively, and in addition, the sample liquid of each detection project is independently added into different sample storage tanks to realize independent sample injection, so that the mutual interference of the detection of each target object is avoided.
The independent sample injection microfluidic immunoassay joint inspection device can simultaneously add the sample liquid into different sample storage tanks at one time, so that the sample liquid can enter a plurality of marker storage tanks and a plurality of item reaction detection tanks through capillary action to carry out detection on a plurality of items, and the detection speed is greatly improved.
The independent sample injection microfluidic immunoassay joint inspection device is provided with the washing supply unit, when reaction detection is finished, the joint inspection strip is pushed to the washing supply unit, the membrane breaking prick at the head end of the joint inspection strip punctures the sealing membrane, cleaning liquid in the liquid washing pipe flows into the joint inspection strip from a channel of the membrane breaking prick for washing, samples, markers and reaction residues remained in the first microfluidic channel, the sample storage tank, the marker storage tank and the item reaction detection tank are sufficiently washed, and then the joint inspection strip is discharged through the second microfluidic channel, so that the detection sensitivity is remarkably improved.
The independent sample introduction microfluidic immunoassay joint inspection device is also provided with a waste liquid absorption and storage unit, a negative pressure valve plate is arranged in the waste liquid absorption and storage unit, when the waste liquid absorption and storage unit slides to the tail end far away from the joint inspection strip, the negative pressure forming valve plate does not move, negative pressure is generated in the inner cavity of the outlet end of the negative pressure forming valve plate and the waste liquid sleeve to suck the second microfluidic channel, and the negative pressure can accelerate the flow of liquid in the first microfluidic channel, the sample storage tank, the marker storage tank, the item reaction detection tank and the second microfluidic channel, so that the reaction speed is increased, and the detection speed is further increased.
The independent sample injection microfluidic immunoassay joint inspection device is simple in structure and easy to operate, meets various application scenes, and reduces medical inspection cost.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic view of the overall structure of an independent sample injection microfluidic immunoassay joint detection device provided by the embodiment of the invention.
Fig. 2 is a schematic structural diagram of a joint test strip of the independent sample feeding microfluidic immunoassay joint test device provided by the embodiment of the invention.
Reference numerals:
the combined test strip 1, the first micro-flow channel 11, the second micro-flow channel 12, the membrane rupture 13, the washing liquid inlet 14, the waste liquid outlet 15, the channel 16, the sample storage tank 17, the sample inlet 171, the washing liquid inlet 172, the marker storage tank 18, the marker inlet 181, the item reaction detection tank 19 and the waste liquid outlet 191.
A washing supply unit 2, a washing liquid sleeve 21, a washing liquid pipe 22, a second sealing ring 23, a sealing film 24 and a chute 25.
The waste liquid sucking and storing unit 3, the waste liquid sleeve 31, the negative pressure forming valve plate 32, the first sealing ring 33 and the connecting rod 34.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
In the description of the present invention, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Referring to fig. 1 to 2, the invention provides an independent sample injection microfluidic immunoassay joint inspection device, which comprises a joint inspection strip 1, a washing supply unit 2 and a waste liquid storage unit 3, wherein the washing supply unit 2 is connected to the head end of the joint inspection strip 1, and the waste liquid storage unit 3 is connected to the tail end of the joint inspection strip 1.
A first micro-fluidic channel 11 and a second micro-fluidic channel 12 are concavely arranged on the joint test strip 1, and the first micro-fluidic channel 11 and the second micro-fluidic channel 12 are arranged along the length direction of the joint test strip 1; one end of the first micro-flow channel 11 is open and communicated with the head end of the joint inspection strip 1, the opening is a washing liquid introducing port 14, and the other end is sealed; one end of the second microflow channel 12 is open and communicated with the tail end of the joint test strip 1, the opening is a waste liquid outlet 15, and the other end is sealed. The head end of the joint test strip 1 is provided with a membrane breaking prick 13, and the membrane breaking prick 13 is communicated with a washing liquid introducing port 14 of the first micro-flow channel 11 through a channel 16. On the joint inspection strip first miniflow channel 11 with set up a plurality of detecting element between the second miniflow channel 12, every detecting element is in the concave sample storage tank 17, a marker storage tank 18 and an item reaction detection groove 19 of establishing on joint inspection strip 1, sample storage tank 17 with set up between the marker storage tank 18 and communicate with each other by injection port 171, it communicates with each other to set up marker entry 181 between marker storage tank 18 and the item reaction detection groove 19, set up on the wall of first miniflow channel 11 a plurality of lotion import 172 respectively with a plurality of sample storage tanks 17 communicate with each other, set up on the wall of second miniflow channel 12 a plurality of waste outlet 191 respectively with a plurality of the item reaction detection groove 19 communicates with each other. Multiple purposeThe label reservoirs 18 contain labels corresponding to the targets, and the item reaction detection chambers 19 have solid phase components fixed to the inner walls thereof for capturing the targets or the labels. The marker and the corresponding solid phase component in the device are independently arranged at different positions, the solid phase components are also independently arranged, and each sample can be independently added into different sample storage tanks 17, so that the mutual interference of each detection item is avoided, and the detection sensitivity is improved. The target may be a protein antigen or antibody, a hapten T3, T4, etc., and the solid phase is divided into an antibody, an antigen, an anti T3 antibody, an anti T4 antibody, etc., which may be an anti-protein antigen. The marker is selected from colloidal gold, colored microspheres, fluorescent substance and lanthanide rare earth element Eu2+And quantum dots. The device is suitable for detection by various detection means.
Set up the tectorial membrane sealed on the upper surface of joint inspection strip 1, make first miniflow channel 11 second miniflow channel 12, a plurality of sample storage tank 17, a plurality of marker storage tank 18 and a plurality of the project reaction detects 19 formation sealed intercommunication chamber way, does benefit to the formation of capillary action and follow-up waste liquid and inhales storage unit 3 formation negative pressure for the liquid flow rate improves the speed and the efficiency of detection.
An observation window is arranged on the wall of the joint inspection strip corresponding to the bottom or the side surface of the plurality of item reaction detection grooves 19. When the observation is carried out by visual observation, the bottom can be seen from the right side, or the side wall can be seen from the side; when the colorimetric detection is used, the color is detected through the side wall; when the fluorescence is used for detection, the side wall and the bottom can be excited right above, and the side wall detects the fluorescence; the side walls and bottom can also be excited with the side walls and the top detects fluorescence. The results of visual and instrumental tests can be reported qualitatively or quantitatively.
The waste liquid sucking and storing unit 3 comprises a waste liquid sleeve 31, a negative pressure forming valve plate 32 and a first sealing ring 33. One end of the waste liquid sleeve 31 close to the joint inspection strip 1 is an inlet end, and the other end of the waste liquid sleeve is an outlet end. The negative pressure forming valve plate 32 is arranged in the inner cavity of the waste liquid sleeve 31, the first sealing ring 33 seals the inlet end of the waste liquid sleeve 31 and is provided with a first hollow matched with the tail end cross section shape of the joint inspection strip 1, the center of the negative pressure forming valve plate 32 is provided with a second hollow matched with the tail end cross section shape of the joint inspection strip 1, the tail end of the joint inspection strip 1 extends into the waste liquid sleeve 31 from the first hollow, the negative pressure forming valve plate 32 is fixedly sleeved on the tail end of the joint inspection strip 1 through the second hollow, and the waste liquid guide outlet 15 of the second channel microflow 12 is communicated with the inner cavity of the outlet end of the waste liquid sleeve 31. The waste liquid sleeve 31 is of a cylindrical structure integrated with injection molding, and the second sealing ring 33 is of a circular structure made of rubber or silica gel.
When the waste liquid sucking and storing unit 3 slides towards the tail end far away from the joint inspection strip 1, the negative pressure forming valve plate 32 does not move, negative pressure is generated in an inner cavity of the outlet end of the negative pressure forming valve plate and the waste liquid sleeve to suck the second microflow channel 12, and then the first microflow channel 11, the plurality of sample storage tanks 17, the plurality of marker storage tanks 18 and the plurality of project reaction detection tanks 19 in the joint inspection strip 1 are sucked, so that the liquid flow can be accelerated, and the detection speed and the detection efficiency are improved.
The washing supply unit 2 includes a washing liquid sleeve 21, a washing liquid pipe 22 and a second sealing ring 23; the liquid washing pipe 22 is arranged in the liquid washing sleeve 21, the second sealing ring 23 seals the inlet end of the liquid washing sleeve 21 and is provided with a third hollow matched with the cross section of the head end of the joint inspection strip 1, the head end of the joint inspection strip 1 extends into the liquid washing sleeve 21 from the third hollow, the opening of the liquid washing pipe 22 is coated with a sealing film 24, and the membrane breaking prick 13 at the head end of the joint inspection strip 1 is opposite to the sealing film 24; the washing liquid pipe 22 is filled with washing liquid; the lotion sleeve 21 is of a cylindrical structure integrated with injection molding, and the first sealing ring 23 is of a circular structure made of rubber or silica gel.
When the joint test strip 1 is pushed to the washing supply unit 2, the membrane rupture 13 punctures the sealing membrane 24, and the cleaning solution in the cleaning solution tube 22 flows from the channel 16 of the membrane rupture 13 into the first microfluidic channel 11 in the joint test strip 1, and then flows into the plurality of sample storage tanks 17, the plurality of marker storage tanks 18, the plurality of item reaction detection tanks 19, and the second microfluidic channel 12 for washing.
The width of the first micro-flow channel 11 and the second micro-flow channel 12 is 1.5-2.5mm, the depth is 1.0-1.5mm, the length is 40-200mm, and the distance between the first micro-flow channel 11 and the second micro-flow channel 12 is 10-15 mm. The depth of the sample storage tank 17 is 1.5-1.7m, the depth of the marker storage tank 18 is 1.8-2.0mm, the depth of the item reaction detection tank 19 is 2.1-2.4mm, and the depths of the sample storage tank 17, the marker storage tank 18 and the item reaction detection tank 19 form gradient increasing, so that the flow of liquid is facilitated, the reaction speed is accelerated, and the detection efficiency is improved.
The first microfluidic channel 11, the second microfluidic channel 12, the sample reservoir 17, the label reservoir 18 and the item reaction detection chamber 19 are all surface-treated to have hydrophilicity, so as to facilitate capillary action to push liquid to flow.
The joint inspection strip 1 is integrally molded by pressing or injection molding of polymer or silicate glass, and is convenient to manufacture.
Waste liquid inhales on the outer wall of the waste liquid sleeve pipe 31 of storage unit 3 along the direction of joint inspection strip 1 stretches out a connecting rod 34, set up a spout 25 on the wall of the lotion sleeve pipe 21 of washing supply unit 2, the end that stretches out of connecting rod 34 inserts will in the spout 25 washing supply unit 2 with the joint of waste liquid inhales storage unit 3 is in the same place, in addition waste liquid sleeve pipe 31 with lotion sleeve pipe 21 is cylindric, and whole independent appearance micro-fluidic immunoassay joint inspection device that advances forms a pen appearance structure like this, carries easily.
The sample inlet 171 is lower than the bottom surface of the sample storage tank 17, so as to facilitate the entry of the sample liquid into the sample storage tank 17.
The waste liquid outlet 191 is higher than the bottom surface of the item reaction detection tank 19, so that the reacted target and marker can stay in the item reaction detection tank 19 for a longer time, fully react with the corresponding solid phase component, fully capture the solid phase component, and improve the detection accuracy and sensitivity.
The number of detection units is 3 to 10, preferably 5. If the number is less than 3, the detection efficiency is too low, and if the number is more than 10, the channels of the first microfluidic channel 11, the second microfluidic channel 12, the sample storage tank 17, the label storage tank 18 and the item reaction detection tank 19 are too long and cannot be cleaned completely, thereby affecting the detection efficiency.
The sample storage tank 17, the marker storage tank 18 and the item reaction detection tank 19 are square grooves, so that injection molding is facilitated.
The prepared independent sample injection microfluidic immunoassay joint inspection device is sealed and stored in a sealing bag.
The use method of the independent sample injection microfluidic immunoassay joint inspection device comprises the following steps:
opening the sealed bag of the independent sample feeding microfluidic immunoassay joint inspection device, adding a sample liquid, such as blood, into each sample storage tank 17, allowing the sample liquid to flow along the sample inlet 171 to the label storage tank 18 under capillary action, allowing a target in the sample liquid to combine with a label in the label storage tank 18 to form a reaction chain mixture, and allowing the reaction chain mixture to flow along the label inlet 181 to the item reaction detection tank 19 under capillary action to sufficiently react with and fix a corresponding solid phase component on the solid phase component. Such a plurality of targets completes the detection reaction.
In the reaction process, the waste liquid storage unit 3 can slide towards the sample outlet end far away from the joint test strip 1 at a speed of 3-6mm/s, the negative pressure forming valve plate 32 does not move, negative pressure is generated in the inner cavity of the outlet end of the negative pressure forming valve plate and the waste liquid sleeve to suck the second micro-flow channel 12, and the liquid in the first micro-flow channel 11, the second micro-flow channel 12, the sample storage tank 137, the marker storage tank 18 and the item reaction detection tank 19 is sucked into the inner cavity of the waste liquid sleeve 31, so that the flow of the liquid is accelerated, and the reaction and detection speed are increased. The sliding speed is very critical, and if the sliding speed is less than 3mm/s, the formed negative pressure is too small, so that the reaction speed is difficult to accelerate; if the sliding speed is higher than 6mm/s, the formed negative pressure is too large, the suction is too fast, the reaction of the target, the marker and the solid phase component is insufficient, and the detection sensitivity is reduced.
After the reaction is completed, the joint test strip 1 is held and pushed towards the washing supply unit 2 until a falling feeling is sensed, at this time, the membrane rupture 13 punctures the sealing membrane 24 of the liquid washing tube 22, and the washing liquid in the liquid washing tube 22 flows into the first microfluidic channel 11 in the joint test strip 1 for washing through the capillary action from the channel of the membrane rupture 13, so as to wash the first microfluidic channel 11, the sample storage tank 17, the marker storage tank 18, the item reaction detection tank 19 and the second microfluidic channel 12 in sequence. The remaining sample solution, the labeling substance, etc. are washed away, and the reaction chain mixture immobilized on the solid phase component is not washed away. In the cleaning process, the waste liquid sucking and storing unit 3 can slide towards the sample outlet end far away from the joint inspection strip 1 at the speed of 8-20mm/s, so that the cleaning liquid and the residual liquid are sucked into the inner cavity of the waste liquid sleeve 31, and the cleaning process is accelerated. This sliding speed is very critical, and if it is less than 8mm/s, it is difficult to clean, and if it is more than 20mm/s, the suction force becomes too large, and the solid phase component is easily extracted, which affects the detection sensitivity.
After washing, the detection result can be observed and calculated through visual inspection, colorimetric or fluorescent counting on the observation window of the joint inspection strip 1, so that interference of residue on detection is avoided, and the detection sensitivity is greatly improved. When the observation is carried out by visual observation, the bottom can be seen from the right side, or the side wall can be seen from the side; when the colorimetric detection is used, the color is detected through the side wall; when the fluorescence is used for detection, the side wall and the bottom can be excited right above, and the side wall detects the fluorescence; the side walls and bottom can also be excited with the side walls and the top detects fluorescence. The results of visual and instrumental tests can be reported qualitatively or quantitatively.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. An independent sample introduction microfluidic immunoassay joint inspection device is characterized by comprising a joint inspection strip, a washing supply unit and a waste liquid absorption and storage unit, wherein the head end of the joint inspection strip is connected with the washing supply unit, and the tail end of the joint inspection strip is connected with the waste liquid absorption and storage unit;
the joint detection strip is concavely provided with a first micro-flow channel and a second micro-flow channel, the first micro-flow channel and the second micro-flow channel are arranged along the length direction of the joint detection strip, one end of the first micro-flow channel is communicated with the head end of the joint detection strip through an opening, the other end of the first micro-flow channel is sealed, one end of the second micro-flow channel is communicated with the tail end of the joint detection strip through an opening, and the other end of the second micro-flow channel is sealed; the head end of the joint inspection strip is provided with a membrane breaking prick, and the membrane breaking prick is communicated with the opening of the first micro-flow channel through a channel; a plurality of detection units are arranged on the joint detection strip between the first micro-flow channel and the second micro-flow channel, each detection unit is provided with a sample storage tank, a marker storage tank and an item reaction detection tank which are concavely arranged on the joint detection strip, sample inlets are arranged between the sample storage tanks and the marker storage tanks for communication, marker inlets are arranged between the marker storage tank and the item reaction detection tanks for communication, a plurality of washing solution inlets are arranged on the wall of the first micro-flow channel for communication with the plurality of sample storage tanks respectively, and a plurality of waste liquid outlets are arranged on the wall of the second micro-flow channel for communication with the plurality of item reaction detection tanks respectively; a film covering seal is arranged on the upper surface of the joint detection strip, so that a sealed communicating cavity channel is formed by the first micro-flow channel, the second micro-flow channel, the plurality of sample storage tanks, the plurality of marker storage tanks and the plurality of item reaction detection tanks; observation windows are arranged on the walls of the joint inspection strips corresponding to the bottoms or the side surfaces of the project reaction detection grooves; the plurality of label storage tanks are respectively filled with labels corresponding to the plurality of targets, and the inner walls of the plurality of item reaction detection tanks are respectively fixedly provided with solid phase components for capturing the plurality of targets or the corresponding labels;
the waste liquid absorbing and storing unit comprises a waste liquid sleeve, a negative pressure forming valve plate and a first sealing ring; the negative pressure forming valve plate is arranged in an inner cavity of the waste liquid sleeve, the first sealing ring seals an inlet end of the waste liquid sleeve and is provided with a first hollow matched with the shape of the cross section of the tail end of the joint inspection strip, a second hollow matched with the shape of the cross section of the tail end of the joint inspection strip is arranged in the center of the negative pressure forming valve plate, the tail end of the joint inspection strip extends into the waste liquid sleeve from the first hollow, the negative pressure forming valve plate is fixedly sleeved on the tail end of the joint inspection strip through the second hollow, and an opening of the second microflow channel is communicated with an inner cavity of an outlet end of the waste liquid sleeve; when the waste liquid sucking and storing unit slides towards the tail end far away from the joint detection strip, the negative pressure forming valve plate does not move, negative pressure is generated in the inner cavity of the outlet end of the negative pressure forming valve plate and the waste liquid sleeve, and the second microflow channel is sucked.
2. The uniflow immunoassay joint inspection device of claim 1, wherein the washing supply unit comprises a washing solution sleeve, a washing solution tube and a second sealing ring; the washing liquid pipe is arranged in the washing liquid sleeve, the second sealing ring seals the inlet end of the washing liquid sleeve and is provided with a third hollow matched with the cross section shape of the head end of the joint detection strip, the head end of the joint detection strip extends into the washing liquid sleeve from the third hollow, the pipe opening of the washing liquid pipe is coated with a sealing film, and a film breaking prick at the head end of the joint detection strip is opposite to the sealing film; the washing liquid pipe is filled with washing liquid; when the joint inspection strip is pushed towards the washing supply unit, the membrane-breaking pricks the sealing membrane, and the cleaning liquid in the liquid washing pipe flows into the first microflow channel in the joint inspection strip from the channel of the membrane-breaking prick for washing.
3. The uniflow immunoassay device of claim 2, wherein the first microfluidic channel, the second microfluidic channel, the sample reservoir, the label reservoir, and the item reaction detection reservoir are surface treated to be hydrophilic.
4. The uniflow immunoassay joint detection device of claim 2, wherein the label is selected from the group consisting of colloidal gold, colored microspheres, fluorescent substances, lanthanide rare earth element Eu2+And quantum dots.
5. The self-contained sample feeding microfluidic immunoassay joint detection device according to claim 2, wherein the joint detection strip is integrally molded by compression molding or injection molding.
6. The device for the joint inspection of the independent sample introduction microfluidic immunoassay of claim 2, wherein a connecting rod extends out of the outer wall of the waste liquid casing of the waste liquid storage unit along the direction of the joint inspection strip, a sliding groove is arranged on the wall of the washing liquid casing of the washing and supplying unit, and the extending end of the connecting rod is inserted into the sliding groove to clamp the washing and supplying unit and the waste liquid storage unit together.
7. The self-contained sample microfluidic immunoassay joint detection device of claim 2, wherein the sample inlet is lower than the bottom surface of the sample reservoir.
8. The uniflow immunoassay device of claim 2, wherein the waste outlet is higher than the bottom surface of the item reaction detection cell.
9. The simultaneous detection device for independent sample injection microfluidic and immunoassay according to claim 2, wherein the number of the detection units is 3 to 10.
10. A method of using the self-contained sample microfluidic immunoassay joint detection device of any one of claims 1 to 9, comprising the steps of:
s1, adding sample liquid into each sample storage tank for reaction;
s2, sliding the waste liquid absorbing and storing unit to the sample outlet end far away from the joint inspection strip at the speed of 3-6 mm/S;
s3, after the reaction is finished, the joint inspection strip is pushed to the washing supply unit until a falling feeling is felt, and at the moment, a membrane rupture prick pierces the sealing membrane of the liquid washing tube to wash;
and S4, observing or calculating the detection result through visual inspection, colorimetric or fluorescent counting on the observation window of the joint inspection strip after the washing is finished.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113281499A (en) * | 2021-05-18 | 2021-08-20 | 厦门先明生物技术有限公司 | Microfluidic immunoassay joint detection device and using method thereof |
| CN114849802A (en) * | 2022-04-29 | 2022-08-05 | 厦门先明生物技术有限公司 | Self-testing type micro-fluidic detection device |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448716A (en) * | 2002-03-28 | 2003-10-15 | 富士胶片株式会社 | Humoral testing unit |
| US20070099290A1 (en) * | 2003-09-02 | 2007-05-03 | Kazuhiro Iida | Customizable chip and method of manufacturing the same |
| CN201421455Y (en) * | 2008-12-30 | 2010-03-10 | 宁波大学 | Special micro-fluidic chip for syphilis diagnosis |
| US20140141999A1 (en) * | 2009-08-03 | 2014-05-22 | Omega Optics, Inc. | Method for Chip-Integrated Label-Free Detection and Absorption Spectroscopy with High Throughput, Sensitivity, and Specificity |
| US20170326551A1 (en) * | 2014-11-26 | 2017-11-16 | Imec Vzw | Fluid Analysis Device |
| CN107855142A (en) * | 2017-11-01 | 2018-03-30 | 深圳市第二人民医院 | A kind of detection chip and detection device based on microflow control technique |
| CN108043478A (en) * | 2017-12-04 | 2018-05-18 | 国家纳米科学中心 | A kind of micro-fluidic chip, manual centrifugal device and nucleic acid detection method |
| CN109975563A (en) * | 2019-03-21 | 2019-07-05 | 厦门先明生物技术有限公司 | A kind of integrated reagent device of microfluid immunoassay |
| CN111077319A (en) * | 2019-12-18 | 2020-04-28 | 华南农业大学 | Micro-fluidic chip immunodetection kit and detection method thereof |
-
2020
- 2020-06-11 CN CN202010530477.6A patent/CN111659477B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448716A (en) * | 2002-03-28 | 2003-10-15 | 富士胶片株式会社 | Humoral testing unit |
| US20070099290A1 (en) * | 2003-09-02 | 2007-05-03 | Kazuhiro Iida | Customizable chip and method of manufacturing the same |
| CN201421455Y (en) * | 2008-12-30 | 2010-03-10 | 宁波大学 | Special micro-fluidic chip for syphilis diagnosis |
| US20140141999A1 (en) * | 2009-08-03 | 2014-05-22 | Omega Optics, Inc. | Method for Chip-Integrated Label-Free Detection and Absorption Spectroscopy with High Throughput, Sensitivity, and Specificity |
| US20170326551A1 (en) * | 2014-11-26 | 2017-11-16 | Imec Vzw | Fluid Analysis Device |
| CN107855142A (en) * | 2017-11-01 | 2018-03-30 | 深圳市第二人民医院 | A kind of detection chip and detection device based on microflow control technique |
| CN108043478A (en) * | 2017-12-04 | 2018-05-18 | 国家纳米科学中心 | A kind of micro-fluidic chip, manual centrifugal device and nucleic acid detection method |
| CN109975563A (en) * | 2019-03-21 | 2019-07-05 | 厦门先明生物技术有限公司 | A kind of integrated reagent device of microfluid immunoassay |
| CN111077319A (en) * | 2019-12-18 | 2020-04-28 | 华南农业大学 | Micro-fluidic chip immunodetection kit and detection method thereof |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113281499A (en) * | 2021-05-18 | 2021-08-20 | 厦门先明生物技术有限公司 | Microfluidic immunoassay joint detection device and using method thereof |
| CN114849802A (en) * | 2022-04-29 | 2022-08-05 | 厦门先明生物技术有限公司 | Self-testing type micro-fluidic detection device |
| CN114849802B (en) * | 2022-04-29 | 2023-08-15 | 厦门先明生物技术有限公司 | Self-testing type micro-fluidic detection device |
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|---|---|
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