CN105842434A - Sample collection and detection apparatus - Google Patents
Sample collection and detection apparatus Download PDFInfo
- Publication number
- CN105842434A CN105842434A CN201610153269.2A CN201610153269A CN105842434A CN 105842434 A CN105842434 A CN 105842434A CN 201610153269 A CN201610153269 A CN 201610153269A CN 105842434 A CN105842434 A CN 105842434A
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- Prior art keywords
- sample
- lid
- chamber
- reaction chamber
- hole
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
Abstract
The invention relates to a sample collection and detection apparatus, comprising a testing element which is composed of a sample receiving zone, a reagent zone and a detection zone. The sample collection and detection apparatus is characterized by further comprising a detection chamber which comprises a base plate and an upper plate, wherein the base plate is snapped with the upper plate to form a hollow detection chamber, the testing element is located in the detection chamber, the upper surface of the upper plate is connected with a sample reaction chamber formed by enclosure of sidewalls and a bottom and having an open end, the bottom of the sample reaction chamber is one part of the upper plate and is provided with a through hole, and the through hole is located above the sample receiving zone of the testing element. The apparatus provided by the invention can realize one-stop completion of collection, buffer reaction and detection of a liquid sample; and in particular, the apparatus can detect a sample needing pretreatment for a period of time and detect the sample at set time.
Description
Technical field
The present invention relates to a kind of sample collection detection device, need to carry out the use of pretreatment especially for sample
In the collection and detection device collecting and detecting sample.
Background technology
Following background technology is adapted to assist in the reader understanding present invention, and is not construed as prior art.
Utilize immunity association reaction principle detects whether there is this technology of analyte in sample by extensively
General it is used in every field.Various biological specimen (saliva, blood, urine, serum, antiperspirant can be detected with it
Liquid etc.) analyte come monitoring of diseases and the mankind health status (early pregnancy, tumor, infectious disease, poison
Product etc.).The cardinal principle of this detection technique is built upon between immune molecule the performance with specific bond,
Such as antibody and antigen, hapten/antibody, biotin and antibiotin etc..
At medical diagnostic field, utilize detection device or detection cup not to collect detection liquid sample, and sentence
Whether disconnected liquid sample contains analyte, is a kind of commonplace method.Such detection device
Or detection cup typically requires that sample is collected in sample container, person skilled includes into a detection examination
Agent bar also makes a part of submergence of reagent strip in the sample, takes out reagent strip and read detection after the some time
Result.
United States Patent (USP) US2004/0184954 and US2004/0237674 disclose some and collect saliva and carry out
Whether detection saliva is contained the device of illicit drugs composition.In the two patent, both provide collection and inspection
Survey the apparatus and method of saliva, in these devices, after sample is sampled on catcher, by applying external force
Sample in absorption piece on catcher is expressed in collecting chamber, detects the most again.But, sample
Catcher is coordinating in extrusion process with collecting chamber, there may be sample contact and pollutes to operator or infect；
Further, in the case of detecting again after running into sample needs elder generation and buffer mixing, such collection device
Just seem not very convenient.
It addition, in some detect, sample needs to be treated and can be detected.Such as, in the sample
Add buffer solution, make sample be diluted reducing viscosity, it is ensured that sample can dialysis smoothly on detector bar
Deng.Buffer solution how can be made to mix smoothly with sample, and mixing is fully and convenient operation is all to need to solve
Problem certainly.
Summary of the invention
The invention provides a kind of novel sample collects the sample collection detection dress that detection integrates
Put, sample can be made to realize collection and buffer agent hybrid reaction and realize detection by this device.
Thering is provided a kind of sample collection detection device in the present invention, including testing element, testing element includes sample
Region of acceptance, reagent area and detection zone, it is characterised in that also include that test chamber, described test chamber include base plate
With upper plate, base plate and upper plate engaging form the test chamber of hollow, described testing element is positioned at test chamber；Institute
State to connect on upper plate and have the sample reaction chamber of the one end open enclosed by sidewall and bottom, wherein, sample
It it is a part for upper plate bottom reaction chamber；A through hole it is provided with bottom described sample reaction chamber；Described through hole position
Top in the sample region of acceptance of testing element.
Some preferred embodiment in, described through hole be provided with seal through hole through hole plug；Original state
Time, through hole is sealed by through hole plug；During detection state, through hole plug is opened and is made through hole be unlocked.During original state
Through hole is sealed by through hole plug and makes test chamber be not attached to lead to sample reaction chamber liquid；During detection state, through hole plug
Open and make through hole be unlocked to make test chamber and sample reaction chamber liquid communication.
Some preferred embodiment in, the described one end open also including being enclosed by sidewall and bottom
Collection well, collection well is positioned at test chamber upper plate one end adjacent to sample reaction chamber.
Some preferred embodiment in, described reaction chamber, collecting chamber and test chamber upper plate be integral type knot
Structure.
Some preferred embodiment in, described collection well is connected with sample reaction chamber and liquid phase
Connection.
Some preferred embodiment in, described collection well sidewall is connected with test chamber upper plate.
One preferred embodiment in, reaction chamber is vertically positioned on upper plate；Described collection well is horizontal
It is positioned on upper plate.Some preferred embodiment in, the bottom of described collection well and the side of reaction chamber
Wall is connected；There is bottom described collection well the through hole being connected with reaction chamber.
In the present invention, cleverly collecting chamber and reaction chamber are fully integrated on the upper plate of test chamber, and
Collecting chamber and reaction chamber also integrate, and make whole sample collection detection device moulding light and handy, and
Enabling sample to collect, pretreatment and detection integral type complete, convenient to operation.Concrete, in order to
Easy to operate, good looking appearance, collecting chamber is mutually perpendicular to reaction chamber, i.e. reaction chamber is vertically positioned at test chamber
On upper plate, and collecting chamber laterally crouches at test chamber upper plate, that is, the bottom of collecting chamber and the side of reaction chamber
Wall links together, and the bottom of reaction chamber is positioned at the top of sample application zone of testing element.
Some preferred embodiment in, also include lid, described lid be used for covering be positioned at sample reaction
Chamber；Lid end connects raised line.
Some preferred embodiment in, also include ring-type buckle, buckle is fixed in lid top side
On wall；Buckle center is provided with projection, covered body wall has and accommodates protruding shrinkage pool, and described buckle is protruding
After snap-on caps body sidewall shrinkage pool, lid interfixes with buckle.
Some preferred embodiment in, the accent of described sample reaction chamber is provided with groove, for receive also
Fixing buckle.Buckle is fixed on sample reaction chamber, and the lid interfixed with buckle reacts at sample
The position in chamber is also fixed, even if also the raised line of lid end is fixed on the surface of through hole plug.
Some preferred embodiment in, lid has primary importance and second on sample reaction chamber
Put；During original state, when lid is positioned at primary importance, buckle is fastened between lid and sample reaction chamber
And fixing lid, lid top not contacting reaction cavity accent, the raised line on lid does not contact sample reaction chamber
Through hole plug on bottom through-hole；During detection state, when lid is positioned at the second position, buckle is removed, lid
Body directly covers on sample reaction chamber after being pressed down, lid top direct contacting reaction cavity accent, lid
On raised line contact and poke the through hole plug bottom sample reaction chamber, make sample reaction chamber be connected with test chamber
Logical.
Some preferred embodiment in, lid end is additionally provided with draw-in groove；In draw-in groove, engaging has chip carrier.
Some preferred embodiment in, lid has pore.This pore can make sample reaction chamber with
Extraneous holding gas is connected, so that the air pressure of sample reaction chamber is identical with atmospheric pressure.One concrete
In embodiment, the aperture of described pore is less, generally between 0.5-2mm.
Some preferred embodiment in, also include sample collection rod, described sample collection rod includes phase
The absorption piece connected and handle；And the seal member between absorption piece and handle.
Some preferred embodiment in, be provided with at collection well accent symmetry two hole clippings；Sample
Collect the one end being connected with absorption piece on bat handle and be provided with two card articles of symmetry.
After sample collection rod insertion collecting chamber carries out sample collection, the absorption piece quilt on sample collection rod
Completely after extruding, on handle, card article then can arrive at the hole clipping at collecting chamber accent, and card article engages with hole clipping
Together, collection rod is made to be locked in collecting chamber, so that the absorption piece collected on rod is constantly in
Compressive state, it is ensured that sample fully extrude outflow, and, sample collection rod is locked in collecting chamber,
Will not be randomly placed, cause environmental pollution.
Beneficial effect
Assembly of the invention can make the collection of liquid sample, and buffering reaction and the one-stop of detection complete, special
It not the detection needing to carry out certain time pretreatment for sample, and the time setting that is capable of enters
Row detection.
Accompanying drawing explanation
Fig. 1 is the decomposing schematic representation of the sample collection detection device of the present invention；
Fig. 2 is the generalized section of the sample collection detection device of the present invention；
Fig. 3 to Fig. 6 is the sample collection detection sample collection of device of the present invention the operating process schematic diagram detected；
Wherein:
Fig. 3 is the sample collection detection device schematic diagram with collecting part released state of the present invention；
Fig. 4 is that the collecting part compression of the present invention is combined in sample collection detection device (lid is in primary importance)
Schematic diagram；
Fig. 5 is that the buckle of the present invention detects, with sample collection, the schematic diagram that device separates；
Fig. 6 is the sample collection detection device schematic diagram of (lid is in the second position) after the lid of the present invention is pressed down；
Fig. 7 to Figure 10 is the sample collection detection sample collection of device of the present invention and the operating process section that detects shows
It is intended to: wherein:
Fig. 7 is the sample collection detection device generalized section with collecting part released state of the present invention；
Fig. 8 is that the collecting part compression of the present invention is combined in sample collection detection device (lid is in primary importance)
Generalized section；
Fig. 9 is that the buckle of the present invention detects, with sample collection, the generalized section that device separates；
Figure 10 is the sample collection detection device section signal of (lid is in the second position) after the lid of the present invention is pressed down
Figure.Description of reference numerals:
Sample collection detection device 800；Test chamber 100；Base plate 102；Upper plate 101；Window 103；Test unit
Part 200；Sample reception district 201；Sample reaction chamber 300；The accent 303 of sample reaction chamber；Sample reacts
Bottom chamber 304；Reaction chamber sidewall 305；Through hole 301 bottom reaction chamber；Reaction chamber accent groove 302；
Through hole plug 310；Lid 400；Lid top 450；Lid end 460；Lid shrinkage pool 401；Carrier 410；
Raised line 420；Pore 430；Collection well 500；The through hole 510 connected with reaction chamber bottom collecting chamber；
Collecting chamber accent 520, collecting chamber sidewall 530；Bottom collecting chamber 540；Hole clipping 501；Buckle 600；Card
Buckle protruding 601；Sample collection rod 700；Collecting part 701；Handle 702；Sealing ring 703；Card article
704；Salient point 705 on card article；
Detailed description of the invention
Below the structure that the present invention relates to or these technical terms used are described further.
Detection
Detection represents whether chemical examination or a kind of material of test or material exist, and such as, but is not limited to this, changes
Learn material, organic compound, inorganic compound, metabolism product, medicine or drug metabolite, have
Machine tissue or the metabolite of organic organization, nucleic acid, protein or polymer.It addition, detection represents tester
Matter or the quantity of material.Furtherly, chemical examination is also represented by immune detection, chemical detection, enzyme detection etc..
Sample
The sample of indication of the present invention refers to those can be used to detect, chemically examine or diagnose whether there is quilt interested
Analyze the material of material.It may be that such as, liquid sample, liquid sample can include blood, blood to sample
Slurry, serum, urine, saliva and various juice, it is also possible to include that solid sample and semi-solid samples are passed through
The liquid solution formed after anticipating.Collect the sample come and may be used for immune detection, chemical detection, enzyme
The methods such as detection detect whether to there is analyte.
Analyte
Any analyte can be analyzed by apparatus and method of the present invention.Analyte can be any
Liquid or liquefied sample detect, such as urine, saliva, saliva, blood, blood plasma, or serum.
Analyte can also is that some hapten materials, and these hapten include drugs (such as Drug abuse)." indiscriminate
With medicine " (DOA) refer to that non-medical destination uses medicine (generally playing the effect of paralysis nerve).Use
This device can be used for detecting and belongs to medical usage but the detection of easy drug overdose, presses down as tricyclic antidepressants is anti-
Strongly fragrant medicine (imipramine or the like) and acetyl aminophenol etc..These medicines can resolve into not after being absorbed by the body
With small-molecule substance, these small-molecule substances be present in the body fluid such as blood, urine, saliva, sweat or
There is above-mentioned small-molecule substance in part body fluid.
Testing element 200
Testing element 200 can select the test strip of horizontal mobility, and it can detect multiple analyte.Certainly,
Other suitable testing elements can also be used in the present invention.Various testing elements can be grouped together fortune
Use in the present invention.A kind of form is Test paper.For analyze analyte in sample (such as drugs or
Show the metabolite of health) Test paper can be various forms, such as immunoassay or chemical analysis
Form.Test paper can use the analytical model of non-competing method or competition law.Test paper comprises a tool
There are the absorbent material of sample region of acceptance, reagent area and test section.Add sample to sample region of acceptance 201, pass through
Capillarity flows to reagent area.In reagent area, if there is analyte, sample is combined with reagent.So
Rear sample continues to flow to detection zone.Other reagent, the molecule such as combination Non-specific with analyte is consolidated
It is scheduled on detection zone.These reagent react with the analyte (if present) in sample and are tied by analyte
It is combined in this district, or is combined with some reagent of reagent area.For showing that the label of detection signal exists
With reagent area or the mark zone that separates.
Typical non-competing method analytical model is: if containing analyte in sample, signal will produce,
If not comprising analyte, the most do not produce signal.In competition law, if analyte is not present in sample
In Ben, signal produces, and if there is analyte, does not the most produce signal.
Testing element 200 is Test paper, can select water suction or the material not absorbed water.Test paper can wrap
Include multiple material to transmit for liquid sample.The material of one of which Test paper can cover at another kind of material
On, as filter paper covers on nitrocellulose filter.One district of Test paper can select one or more materials
Material, and another district selects one or more materials that other are different.Test paper can be attached on certain
Hold thing or hard surface for improving the intensity being affectedly bashful Test paper.Analyte passes through signal generating system
And be detected, as utilized one or more enzymes with this analyte generation specific reaction, utilize as aforementioned
The method being fixed on Test paper by specific combinating substance, by the combination of one or more signal generating systems
Thing is fixed on the analyte detection zone of Test paper.Produce signal material can at sample region of acceptance 201,
On reagent area, or detection zone, or whole Test paper, this material can be full of the one of Test paper or many
Plant on material.Solution containing signal thing is added to the surface of reagent paper or one or more materials of reagent paper are soaked
Not in the solution containing signal thing.The reagent paper making addition contain signal thing solution is dried.
Each district of Test paper can arrange in the following manner: sample region of acceptance 201, reagent area, detection
District, control zone, determine the most adulterated district of sample, liquid sample uptake zone.After control zone is positioned at detection zone.
All of district can be arranged at only with on a reagent paper of a kind of material.May also be not same district and use different
Material.Each district can directly contact with liquid sample, or different districts is according to the direction of liquid sample flowing
Arrangement, is connected the end in each district with the front end in another district and overlaps.Material used can be water absorption relatively
Good material such as filter paper, glass or nitrocellulose filter etc..Test paper can also use other forms.
Sample collection rod 700
Generally, sample collection rod includes absorption piece 701 and handle 702, and absorption piece 701 is positioned at handle
One end of 702.Absorption piece is generally by the other sponge of medical grade commonly used in the art or plastic foam material system
Become.But many other materials may be made as absorption piece, such as Cotton Gossypii or paper, or other any tools
There is the material of water absorbing properties.Handle is typically rigidity, is conducive to the operation to absorption piece.Handle is permissible
It is made up of material commonly used in the art, such as plastics, timber, metal or cardboard.In certain embodiments,
In one end that handle is connected with absorption piece, also there is O-ring seals 703, this sealing ring be surrounded on handle with
The junction of absorption piece.For sealing the collecting chamber accepting to collect rod, make the liquid sample that is located therein not
Understand reflux to handle.In some modes being more highly preferred to, in order to make the liquid in absorption piece be extruded
More thorough, meanwhile, also avoid sample collection rod to be randomly placed, sample collection rod is provided with the card of symmetry
Bar 704, these card articles have elasticity, are compressed after entering collecting chamber, after arriving certain position, card article
On salient point 705 touch and be released after hole clipping on collecting chamber sidewall, salient point enters in hole clipping engaged fixing.
Test chamber 100
A test chamber 100 usually cavity, is used for accommodating testing element 200, and enables liquid sample
Enter engaged test element in this cavity to detect.Its various shapes, the test that can accommodate as required
Shape and the quantity of element are designed.In the present invention, testing element is test strips, therefore, and an enforcement
In example, test chamber is plank frame, has base plate 102 and upper plate 101, and the two is tied by the way of engaging
A cavity 100 is formed after being combined, more specifically, after test strips 200 is placed on base plate 102,
Cover upper plate 101, upper plate 101 is fixed on base plate 102, form test chamber 100.Specifically
, base plate 102 has the strip-shaped clamp groove of one or more projection, these draw-in grooves are for placing and fixing examination
Paper slip 200.In other limited embodiments, upper plate has a window 103, window 103
Put corresponding with the detection zone of test strips, the convenient test result observing test strips.
Sample reaction chamber300
In some detects, sample needs to be processed in advance before detected, such as, is diluted, or
Need to carry out reaction in advance with a certain reagent make analyte therein be activated or be labeled, therefore sample
Reaction chamber 300, while storing liquid sample, makes liquid sample realize in this cavity and buffer solution
Mixing, or make a certain reagent and liquid sample carry out pre-reaction so that liquid sample is in sample reaction chamber
Pretreated.In specific embodiment, sample reaction chamber 200 is enclosed the tubular of hollow by bottom and sidewall
Structure.
In the following detailed description, the subsidiary reference word of legend is a part here, and it is with citing
Illustrate that the present invention can the mode of actable specific concrete scheme illustrate.We are not precluded from the present invention and also may be used
To carry out other concrete scheme and at the knot changing the present invention in the case of the range of the present invention
Structure.
First, the sample testing apparatus of the present invention mainly includes sample reaction chamber 300 and test chamber 100.Its
In, test chamber 100 is used for disposing testing element 100, receives liquid sample simultaneously, makes entrance test chamber 100
Liquid sample sequentially flow through testing element 200, it is achieved detection.And sample reaction chamber 300, it is used for storing
Liquid sample, and make liquid sample realize mixing with buffer solution in this cavity 300, or make a certain
Reagent and liquid sample carry out pre-reaction so that liquid sample is pretreated in sample reaction chamber 300.
In order to realize detection function, test chamber 100 and sample reaction chamber 300 also need to liquid phase circulation, i.e. make
Sample reacts, in the liquid fluid in 300 to test chamber 100 and arrive in test strips 200.Therefore, detection
A passage it is additionally provided with between chamber 100 and reaction chamber 300.Because liquid sample needs to process in advance, and
The most pretreated sample can not detect, so, this passage can not make reaction chamber 300 and detection
Chamber 300 is constantly in connected state, say, that this passage is closed when original state and made test chamber 100
It is not attached to lead to sample reaction chamber 300 liquid；Passage is opened when the state of detection and is made test chamber 100 react with sample
Chamber 300 liquid communication.Therefore, the liquid when needs detect how is realized between test chamber 100 and reaction chamber 300
Body just circulates becomes crucial.
In the present invention, as it is shown in figure 1, test chamber 100 is mutually clamped is formed by base plate 102 and upper plate 101, reagent paper
Bar 200 is fixed in test chamber 100 between base plate 102 and upper plate 101, in test strips sample application zone
The corresponding position, top of 201, upper plate 101 connects and has a cavity 300, and this cavity 300 is by sidewall 305 and bottom
304 enclose and form, and have opening 303, are also a part for test chamber upper plate 101 bottom cavity 300；This chamber
Body 300 is columnar structured, is vertically positioned on test chamber upper plate 101, as shown in Figure 2.Bottom reaction chamber 300
Being provided with a through hole 301, this through hole 301 makes to connect between reaction chamber 300 with test chamber 100, more specifically,
Through hole 301 is positioned at the top of the sample region of acceptance 201 of testing element；When original state, through hole 301 is sealed,
When needs detect, make through hole 301 be unlocked and make to connect between reaction chamber 300 with test chamber 100.Through hole 301
The mode sealed does not limits, and opening when needed, and does not produces the detection of liquid sample
The material of impact can, therefore, it is possible to use diaphragm seal, it is possible to use various sealing-plugs etc., one
In specific embodiment, utilizing through hole plug 310 to seal through hole, the material of through hole plug is silica gel.Tested at sample
Before survey, through hole plug 310 is positioned at through hole and seals through hole, and the liquid sample in reaction chamber 300 can not arrive test chamber
100, after band sample is pretreated or when needing sample is detected, through hole 301 is opened, namely takes off logical
Stopple 310 or make through hole plug 310 by perforate.In some specific embodiments, in order to easy to operate, through hole plug
310 make through hole open by the way of being punctured.
More specifically in embodiment, this detection device also includes a lid covered on sample reaction chamber 300
Body 400, and the raised line 420 being fixedly connected on lid, poke or puncture through hole plug 310 by raised line 420.
This raised line 420 is connected to the end 460 of lid 400, when raised line 420 is positioned at reaction chamber 300, and raised line 420
It is positioned at the surface of through hole plug 310, in order to make raised line 420 can poke through hole plug 310, raised line 420 when needed
Length slightly larger than or equal to the height of reaction chamber 300.Meanwhile, in order to just make raised line 420 puncture when needed
Through hole plug 310, needs raised line 420 not contact through hole plug 310 when initial position, in an embodiment, at lid
Also being surrounded with a ring-type buckle 600 on body 400, this ring-type buckle 600 is positioned at the top 450 of lid 400, when
After lid 400 covers reaction chamber 300, buckle 600 is positioned at the accent 303 at lid top 450 and reaction chamber 300
Between, make lid top 450 not contact with reaction chamber accent 303, as shown in Figure 3 and Figure 4, also make to be connected to lid
The end of the raised line 420 on body not contact through hole plug 310, as shown in Figure 7.When initial position, namely lid
400 when being positioned at primary importance, and buckle 600 is fastened between lid top 450 and reaction chamber accent 303, is positioned at anti-
Answer the not contact through hole plug 310 of the raised line 420 in chamber 300；When needs detect, take off buckle 600, such as Fig. 5 institute
Show, make lid 400 be moved downward to lid top 450 contacting reaction cavity accent 303, as shown in Figure 6, i.e. lid
400 arrive the second position, during this, along with the end contact of the projection 420 moved down together of lid 400
And puncture through hole plug 310, as shown in Figure 10.In some embodiments, in order to make raised line 420 to be positioned at accurately
The surface of through hole plug 310 so that raised line 420 can poke through hole plug 310 smoothly, needs fixing lid 400
Position on reaction chamber 300, so that the position of raised line 420 is fixed, fixes lid 600 by buckle 600, with
Make lid 400 can not on reaction chamber 300 random change of location, more specifically, the center of ring-type buckle 600
Position has protruding 601, and the sidewall corresponding position at lid top 450 has shrinkage pool 401, when buckle is protruding
After 601 insert covered body wall shrinkage pool 401, the position between buckle 600 and lid 400 interfixes.One more
In specific embodiment, on the accent 303 of reaction chamber, also having a groove 302, this groove 302 is used for
Placing a part for annular buckle 600, more specifically, on buckle, the part of protruding 601 junctions is positioned at reaction
In the groove 302 of chamber accent.The position making buckle 600 is fixed, even if also fixing with buckle lid 400
Position is fixed such that it is able to realizes the raised line 420 on lid and is just being positioned at the through hole 301 of reaction chamber accurately
Top.
In order to make the liquid sample in reaction chamber 300 pretreated, can add in reaction chamber 300 buffer or
Other reagent, in an embodiment, by reagents loaded on carrier 410, this carrier 410 is pre-placed in
In reaction chamber 300, to facilitate operation.More specifically, lid 400 has draw-in groove, by carrier 410 1
End is fixed in draw-in groove, and after lid 400 covers reaction chamber, carrier 410 is positioned at reaction chamber 300.
In some embodiments, lid 400 is additionally provided with a pore 430, makes reaction chamber 300 and ambient atmosphere
It is connected.
Secondly, the sample collection that the present invention provides detects device 800 except including detecting the test chamber 100 of device,
Sample reaction chamber 300, also includes collection well 500.In one embodiment, test chamber 100 and reaction chamber 300
And collection well 500 is integral type structure.That is, same, test chamber 100 is also by base plate 102 and upper plate
101 engagings form, and testing element 200 is positioned at the draw-in groove of base plate 102 to be fixed, and wherein testing element 200 is multiple,
More specifically, testing element is test strips, and this test strips is provided with sample region of acceptance 201, reagent area and detection
District.Sample reaction chamber 300 is for being vertically positioned on upper plate 101, and its position is at the sample region of acceptance 201 of test strips 200
Surface, reaction chamber 300 is, to be added as bottom 304 by one end (sample receiving terminal) of upper plate 101
The hollow that sidewall 305 encloses columnar structured, its opening is accent 303.The bottom of reaction chamber 300
(upper plate 101) is provided with through hole 301, as in figure 2 it is shown, this through hole 301 is positioned at testing element 200 (test strips)
The top of sample region of acceptance 201.When liquid flows out to test chamber 100 from reaction chamber 300 is by through hole 301
Time, the sample region of acceptance 201 of liquid first engaged test element.As it is shown in figure 1, collection well 500 is same
Being the cavity of the one end open 520 enclosed by sidewall 530 and bottom 540, this cavity 500 is positioned at detection
One end of the upper plate 101 in chamber 100, is connected as a single entity with upper plate 101, meanwhile, and collecting chamber 500 and reaction chamber 300
Linking together, concrete, the opening direction of collecting chamber is mutually perpendicular to the opening direction of reaction chamber, i.e. collects
Chamber 500 is laterally positioned on test chamber upper plate 101, namely collecting chamber sidewall 530 is connected with test chamber upper plate 101,
Namely the bottom of collecting chamber 500 is connected with the sidewall of reaction chamber 300, more specifically, the end of collecting chamber 500
Portion is the sidewall of reaction chamber 300 simultaneously.In the bottom of collecting chamber 500, it is provided with through hole 510 and makes collecting chamber 500 with anti-
Chamber 300 is answered to connect.
This sample collection detection device 800 also includes sample collection rod 700, collects rod 700 and mainly includes absorption portion
Part 701 and handle 702, the two is connected, and draws sample by absorption piece 701, such as, puts into and is examined
Person oral cavity is drawn saliva sample, is affectedly bashful collection rod by handle 702.
Collecting chamber 500 is combined with collection rod 700 and makes the liquid sample on absorption piece 701 outflow, tool
For body, make absorption piece 701 insert in collecting chamber 500, and move to collecting chamber 500 base portion, make
Absorption piece 701 contacts bottom collecting chamber 500 and is compressed, and the sample being positioned in absorption piece 701 is squeezed
Pressure passes out, and flow in collecting chamber 500.Preferably, in order to make liquid will not outflow collecting chamber 500,
There is in absorption piece 701 and handle 702 junction the sealing ring 703 of annular, as shown in Figures 2 and 3, be somebody's turn to do
Sealing ring 703 seals the sidewall of collecting chamber 500 collecting after rod 700 inserts collecting chamber 500, make collecting chamber
500 form a cavity sealed, it is to avoid liquid outflow, meanwhile, along with the movement of collection rod 700, and should
Sealing cavity volume is gradually reduced, and makes the pressure in annular seal space body increase, and is more beneficial for extruding absorption piece
701 let the liquid out.In some embodiments, when the absorption piece 701 collecting rod is all compressed in collection
After intracavity, collect rod 700 and be locked in collecting chamber 500, in specific embodiment, handle 702 and suction
The one end receiving parts 701 connection is designed with two symmetrical card articles 704, and this card article 705 has elasticity, energy
Enough by compression and collecting chamber 500.Collecting chamber 500 has 2 symmetrical cards on the sidewall at accent
Hole 501, after absorption piece 701 is all compressed, the salient point 705 on card article is placed exactly in hole clipping 501
Fixed, make collection rod 700 be locked in collecting chamber 500.
First sample flows in collection well 500 after being extruded from absorption piece 701, then passes through through hole
510 are flowed in sample reaction chamber 300.Sample collection in the present invention detects on device 800, and sample is anti-
Answer and on chamber 300, be covered with lid 400, lid has pore 430, in this pore 430 makes reaction chamber 300
Outer air is identical, and i.e. keeping the air pressure in reaction chamber 300 is atmospheric pressure, so, when quilt in collecting chamber 500
Collect the liquid flowed out after rod 700 seals and compresses and be more than the air pressure in reaction chamber because of air pressure, make liquid-like
This flows into more smoothly by through hole 510.There is in the draw-in groove of lid 400 carrier 410, this carrier 410
Being positioned at reaction chamber 300, carrier 410 is loaded with the reagent of pretreatment sample, when liquid sample flows into sample
After reaction chamber 300, contact carrier 410 reacts, it is achieved pretreatment.At lid 400 top, there is card
Button 600, after the lid 400 with buckle covers on reaction chamber 300, is positioned at the raised line of lid end
420 surfaces being positioned at reaction chamber bottom through-hole 301, but the not through hole plug 310 on contact through hole, when taking
After lower buckle 600, lower cover body 400, make lid 400 top contact with the accent of sample reaction chamber 300,
During this, raised line 420 contacts and pokes through hole plug 310, the most pretreated in making reaction chamber 300
Liquid sample flow into test chamber 100, with the sample region of acceptance of test strips by the through hole plug 310 that is punctured
201 contacts, complete detection.
Sample collection detection device 800 with regard to the present invention carries out the collection of sample and detects quilt in sample below
The method of analyte is described.
1. when initial position, as shown in Figure 3 and Figure 7, on the sample collection detection device 800 of integral structure,
Annular buckle 600 connects covered body wall shrinkage pool 401 and reaction respectively by protruding 601 and protruding junction
Chamber groove 302 is fixed, and lid 400 is positioned at primary importance, and now, the raised line 420 of lid end is positioned at
In reaction chamber 300, directly over through hole plug 310, do not contact with through hole plug 310；Now, rod is collected
700 separate with collecting chamber 500；
2. collection is had absorption piece 701 one end collecting rod 700 of liquid sample towards horizontal collecting chamber 500
It is inserted into, after absorption piece 701 fully enters collecting chamber 500, is connected to absorption piece 701 end
Sealing ring 703 seal sidewall at collecting chamber 500 accent, make collecting chamber 500 formed seal cavity；
3. continue to advance to collect rod 700, make absorption piece 701 be collected chamber 500 bottom squeeze compression, absorption portion
Liquid on part 701 flows out in collecting chamber 500；Meanwhile, by collecting chamber 500 and reaction chamber 300
Through hole 501 flow in reaction chamber 300；
4., after absorption piece 701 is all extruded, the salient point 705 of the card article 704 being positioned on handle is engaged to receive
In the bayonet socket 501 of collection chamber sidewall, collect rod 700 and be locked in collecting chamber 500, such as Fig. 4 and Fig. 8 institute
Show；
5. make sample collection detection device 800 stand 3-5 minute, now, be positioned at the liquid-like of reaction chamber 300
This contacts with the reagent on carrier 410 and reacts, and completes the pretreatment of sample；
6. take off the annular buckle 600 being fastened on lid 400, as shown in figs. 5 and 9；
7. press down on lid 400, during pressing, the pore 430 of lid is closed；In the process,
Raised line 420 moves bottom reaction chamber 300 with lid 400, comes into contact with and punctures through hole plug
310；
8., when the top contact of lid 400 is to time at the accent of reaction chamber 300, stop pressing, now, lid
Body 400 is positioned at the second position, and now, through hole plug 310 is punctured, the liquid in reaction chamber 300
Sample via through holes 301 enters the sample region of acceptance 201 of the test strips 200 in test chamber 100, enters
Row detection.
Claims (15)
1. a sample collection detection device, including testing element, testing element includes sample region of acceptance, reagent area and detection
District, it is characterised in that also include that test chamber, described test chamber include base plate and upper plate, base plate and upper plate engaging shape
Becoming the test chamber of hollow, described testing element is positioned at test chamber；Connect to have on described upper plate and enclosed by sidewall and bottom
The sample reaction chamber of the one end open closed, wherein, is a part for upper plate bottom sample reaction chamber；Described sample
A through hole it is provided with bottom this reaction chamber；Described through hole is positioned at the top of the sample region of acceptance of testing element.
Sample collection the most according to claim 1 detection device, it is characterised in that described through hole is provided with sealing through hole
Through hole plug；During original state, through hole is sealed by through hole plug；During detection state, through hole plug is opened and is made through hole be opened
Open.
Sample collection the most according to claim 1 detection device, it is characterised in that described also include by sidewall and bottom
The collection well of the one end open enclosed, collection well is positioned at test chamber upper plate adjacent to sample reaction chamber
One end.
Sample collection the most according to claim 3 detection device, it is characterised in that described reaction chamber, collecting chamber and inspection
Surveying chamber upper plate is integral type structure.
Sample collection the most according to claim 3 detection device, it is characterised in that described collection well is anti-with sample
Chamber is answered to be connected and liquid communication.
Sample collection the most according to claim 5 detection device, it is characterised in that described collection well sidewall and inspection
Survey chamber upper plate to be connected.
Sample collection the most according to claim 6 detection device, it is characterised in that the bottom of described collection well with
The sidewall of reaction chamber is connected；There is bottom described collection well the through hole being connected with reaction chamber.
Sample collection the most according to claim 2 detection device, it is characterised in that also including lid, described lid is used
It is positioned at sample reaction chamber in covering；Lid end connects raised line.
Sample collection the most according to claim 8 detection device, it is characterised in that also include ring-type buckle, card buckle
Conjunction is fixed on the sidewall of lid top；Buckle center is provided with projection, covered body wall has and accommodates protruding shrinkage pool,
After described buckle projection snap-on caps body sidewall shrinkage pool, lid interfixes with buckle.
Sample collection the most according to claim 2 detection device, it is characterised in that the accent of described sample reaction chamber sets
Fluted, it is used for receiving and fixing buckle.
11. sample collection according to claim 9 detection devices, it is characterised in that lid has on sample reaction chamber
Primary importance and the second position；During original state, when lid is positioned at primary importance, buckle is fastened on lid and sample
Between reaction chamber and fixing lid, lid top not contacting reaction cavity accent, it is anti-that the raised line on lid does not contact sample
Answer the through hole plug on the bottom through-hole of chamber；During detection state, when lid is positioned at the second position, buckle is removed, lid
Directly cover after being pressed down on sample reaction chamber, lid top direct contacting reaction cavity accent, the raised line on lid
Contact and poke the through hole plug bottom sample reaction chamber, make sample reaction chamber be connected with test chamber.
12. sample collection according to claim 8 detection devices, it is characterised in that lid end is additionally provided with draw-in groove；Card
In groove, engaging has chip carrier.
13. sample collection according to claim 8 detection devices, it is characterised in that there is on lid pore.
14. sample collection according to claim 3 detection devices, it is characterised in that also include sample collection rod, described
Sample collection rod include the absorption piece and the handle that are connected；And the sealing between absorption piece and handle
Parts.
15. sample collection according to claim 14 detection devices, it is characterised in that be provided with at collection well accent
Two symmetrical hole clippings；The one end being connected with absorption piece on sample collection bat handle is provided with two card articles of symmetry.
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Address after: 310011 Four 3-storey Building No. 1418-50 Moganshan Road, Hangzhou City, Zhejiang Province (Shangcheng Science and Technology Industrial Base) Patentee after: Assure Tech. (Hangzhou) Co., Ltd. Address before: 310030 Building 6, No. 10 Xiyuan Third Road, Sandun Town, Xihu District, Hangzhou City, Zhejiang Province Patentee before: Assure Tech. (Hangzhou) Co., Ltd. |
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Address after: 310011 3 / F, building 4, No. 1418-50, Moganshan Road, Hangzhou City, Zhejiang Province (Shangcheng science and technology industrial base) Patentee after: Hangzhou anxiu Biotechnology Co., Ltd Address before: 310011 3 / F, building 4, No. 1418-50, Moganshan Road, Hangzhou City, Zhejiang Province (Shangcheng science and technology industrial base) Patentee before: ASSURE TECH. (HANGZHOU) Co.,Ltd. |