Invention content
The present invention provides a kind of sample collection detection devices of the collection detection together as one of novel sample, by this
Device can make sample realize collection and buffer hybrid reaction and realize detection.
A kind of sample collection detection device, including testing element be provided in the present invention, testing element include sample region of acceptance,
Reagent area and detection zone, which is characterized in that further include test chamber, the test chamber includes bottom plate and upper plate, bottom plate and upper board
Conjunction forms hollow test chamber, and the testing element is located in test chamber;It is connected on the upper plate by side wall and bottom enclosing
Made of one end open sample reaction chamber, wherein sample reaction chamber bottom be upper plate a part;Sample reaction chamber bottom
Portion is set there are one through-hole;The through-hole is located at the top of the sample region of acceptance of testing element.
In some preferred embodiments, the through-hole is equipped with the through hole plug of sealing through-hole;When original state, through-hole quilt
Through hole plug seals;When detecting state, through hole plug opening makes through-hole be opened.Through-hole makes detection by through hole plug sealing when original state
Chamber is not attached to lead to sample reaction chamber liquid;When detecting state, through hole plug opening, which makes through-hole be opened, keeps test chamber anti-with sample
Answer chamber liquid communication.
In some preferred embodiments, described further includes that the sample of one end open made of being enclosed by side wall and bottom is received
Collect chamber, collection well is located at test chamber upper plate adjacent to one end of sample reaction chamber.
In some preferred embodiments, the reaction chamber, collecting chamber and test chamber upper plate are an integral structure.
In some preferred embodiments, the collection well is connected with sample reaction chamber and liquid communication.
In some preferred embodiments, the collection well side wall is connected with test chamber upper plate.
In one preferred embodiment, reaction chamber is vertically positioned on upper plate;The collection well is laterally positioned in upper plate
On.In some preferred embodiments, the bottom of the collection well is connected with the side wall of reaction chamber;The sample collection
Bottom of chamber portion has the through-hole being connected with reaction chamber.
In the present invention, cleverly by collecting chamber and reaction chamber be fully integrated on the upper plate of test chamber and collecting chamber and
Reaction chamber also integrates, and keeps entire sample collection detection device moulding light and handy, and sample is enable to collect, pretreatment
And detection integral type is completed, it is easy to operate.Specifically, in order to easy to operate, good appearance, collecting chamber is mutual with reaction chamber
Vertically, i.e., reaction chamber is vertically positioned on test chamber upper plate, and collecting chamber laterally crouches in test chamber upper plate, that is, the bottom of collecting chamber
The side wall of portion and reaction chamber links together, and the bottom of reaction chamber is located at the top of the sample application zone of testing element.
Further include lid in some preferred embodiments, the lid is located at sample reaction chamber for covering to close;Lid end
End is connected with raised line.
In some preferred embodiments, further includes cyclic annular buckle, be fixed in lid top sidewall;Buckle
Center is equipped with protrusion, has on covered body wall and accommodates raised shrinkage pool, after the buckle protrusion engaging covered body wall shrinkage pool, lid
It is fixed to each other with buckle.
In some preferred embodiments, the accent of the sample reaction chamber is equipped with groove, for receiving simultaneously fixed card buckle.
Buckle is fixed on sample reaction chamber, and the lid being fixed to each other with buckle is also fixed in the position of sample reaction chamber, namely
The raised line of lid end is set to be fixed on the surface of through hole plug.
In some preferred embodiments, lid has first position and the second position on sample reaction chamber;Initial shape
When state, when lid is located at first position, it is fastened between lid and sample reaction chamber and fixed lid, lid top does not connect
Reaction chamber accent is touched, the raised line on lid does not contact the through hole plug on sample reaction chamber bottom through-hole;When detecting state, lid position
When the second position, buckle is removed, and directly lid closes on sample reaction chamber after lid is pressed down, and is in direct contact at the top of lid anti-
Chamber accent, the raised line on lid is answered to contact and poke the through hole plug of sample reaction chamber bottom, make sample reaction chamber and test chamber phase
Connection.
In some preferred embodiments, lid end is additionally provided with card slot;Engaging has chip carrier in card slot.
In some preferred embodiments, there is stomata on lid.The stomata can be such that sample reaction chamber is kept with extraneous
Gas is connected, to keep the air pressure of sample reaction chamber identical as atmospheric pressure.In one specific embodiment, the hole of the stomata
Diameter is smaller, usually between 0.5-2mm.
Further include sample collection stick in some preferred embodiments, the sample collection stick includes the suction being connected
Receive component and handle;And the seal member between absorption piece and handle.
In some preferred embodiments, symmetrical two card holes are equipped at collection well accent;Sample collection stick hand
The one end being connect with absorption piece on handle is equipped with symmetrical two strips.
After sample collection stick, which is inserted into collecting chamber, carries out sample collection, the absorption piece on sample collection stick is squeezed completely
Afterwards, strip can then reach at the card hole at collecting chamber accent on handle, and strip is fastened togather with card hole, keep collection stick locked
In collecting chamber, to make the absorption piece on collection stick be constantly in compressive state, ensure the abundant extruding outflow of sample, and
And sample collection stick is locked in collecting chamber, will not be randomly placed, be caused environmental pollution.
Advantageous effect
The device of the invention can make the collection of liquid sample, the one-stop completion of buffering reaction and detection, especially needle
Sample is needed to carry out certain time pretreated detection, and can realize that the time in setting is detected.
Specific implementation mode
Structure of the present invention or these used technical terms are described further below.
Detection
Detection indicates chemical examination or tests a kind of substance or material whether there is, for example, but it is not limited to this, chemical substance,
Organic compound, inorganic compound, metabolism product, drug or drug metabolite, organic organization or organic organization generation
Thank object, nucleic acid, protein or polymer.In addition, detection indicates test substances or the quantity of material.Furtherly, table is gone back in chemical examination
Show immune detection, chemical detection, enzyme detection etc..
Sample
Signified sample of the invention refers to those and can be used for detecting, chemically examine or diagnosing whether there is interested analyte
The substance of matter.Sample can be, for example, liquid sample, liquid sample may include blood, blood plasma, serum, urine, saliva and
Various juices can also include the liquid solution that solid sample and semi-solid samples are formed after anticipating.It collects and
Sample can be used for the methods of immune detection, chemical detection, enzyme detection and detect whether that there are analytes.
Analyte
Any analyte can be analyzed with apparatus and method of the present invention.Analyte can in any liquid or
It is detected in person's liquefied sample, such as urine, saliva, saliva, blood, blood plasma or serum.Analyte can also be one
A little haptens substances, these haptens include drugs (such as drug abuse)." drug abuse " (DOA), which refers to non-medical destination, to be made
With drug (usually playing paralysis nerve).Detection, which is can be used for, using the device belongs to medical usage but easy medication
Excessive detection, such as tricyclic antidepressant (imipramine or the like) and Paracetamol.These drugs are absorbed by the body
Different small-molecule substances can be resolved into afterwards, these small-molecule substances be present in the body fluid such as blood, urine, saliva, sweat or
There are above-mentioned small-molecule substances for part body fluid.
Testing element 200
Testing element 200 can select the test strip of lateral flow, it can detect a variety of analytes.Certainly,
He can also be used in the present invention by suitable testing element.Various testing elements can be grouped together and apply to the present invention
In.A kind of form is Test paper.For analyzing the analyte in sample (such as drugs or show the metabolin of physical condition)
Test paper can be various forms, such as immunoassays or the form of chemical analysis.Non-competing method may be used in Test paper
Or the analytical model of competition law.Test paper includes a water-absorbent material with sample region of acceptance, reagent area and test section.Sample-adding
This flows to reagent area to sample region of acceptance 201 by capillarity.In reagent area, if there is analyte, sample and examination
Agent combines.Then sample continues to flow to detection zone.Other reagents, such as molecule with the Non-specific combination of analyte are fixed
In detection zone.These reagents react with the analyte (if present) in sample and analyte are incorporated in the area, or
It is combined with some reagent of reagent area.For showing that the marker of detection signal exists and reagent area or the mark zone detached.
Typically non-competing method analytical model is:If containing analyte in sample, signal just will produce, if do not wrapped
Containing analyte, signal is not just generated.In competition law, if analyte is not present in sample, signal generates, if deposited
In analyte, then signal is not generated.
Testing element 200 is Test paper, the material that can be selected water suction or not absorb water.Test paper may include a variety of materials
Material is transmitted for liquid sample.The material of one of which Test paper can cover on another material, as filter paper is covered in nitre
On acid cellulose film.One area of Test paper can select one or more materials, and another area select that other are different one
Kind or multiple material.Test paper can be attached on certain support or hard surface and be affectedly bashful Test paper for improving
Intensity.Analyte is detected by signal generating system, such as utilizes one kind that specific reaction occurs with this analyte
Or a variety of enzymes, specific combinating substance is fixed on the method on Test paper using such as aforementioned, one or more signals are occurred
The composition of system is fixed on the analyte detection zone of Test paper.The substance for generating signal can be tried in sample region of acceptance 201
On agent area or detection zone, or entire Test paper, which can be full of on one or more materials of Test paper.It will contain
There is the solution of signal object to be added to the surface of test paper or one or more materials of test paper are immersed in the solution of the object containing signal.Make
The test paper drying of the solution of object containing signal is added.
Each area of Test paper can arrange in the following manner:Sample region of acceptance 201, reagent area, detection zone, control
Area determines the whether adulterated area of sample, liquid sample uptake zone.Control zone is located at after detection zone.All areas can be arranged
On a test paper only with a kind of material.It can also be not same district and use different materials.It each area can direct and liquid-like
The direction arrangement of this contact or different areas according to liquid sample flowing, the end in each area is connected simultaneously with the front end in another area
It is overlapping.Material used can be the preferable material of water imbibition such as filter paper, glass or nitrocellulose filter etc..Test paper
Other forms may be used.
Sample collection stick 700
In general, sample collection stick includes absorption piece 701 and handle 702, absorption piece 701 is located at one end of handle 702.
Absorption piece is usually made of the other sponge of medical grade commonly used in the art or plastic foam material.But many other materials
It can be made into absorption piece, such as cotton either paper or other any materials with water absorbing properties.Handle is typically rigidity
, be conducive to the operation to absorption piece.Handle can be made of material commonly used in the art, for example, plastics, timber, metal or
Cardboard.In some embodiments, in one end that handle is connect with absorption piece also there are O-ring seals 703, the sealing ring to surround
In the junction of handle and absorption piece.For sealing the collecting chamber for receiving to collect stick, make the liquid sample being located therein will not
In reflux to handle.Some are more preferably in mode, in order to make that the liquid in absorption piece is extruded more thoroughly, meanwhile,
Also it avoiding sample collection stick from being randomly placed, symmetrical strip 704 is equipped on sample collection stick, these strips have elasticity,
It is compressed after entering collecting chamber, after reaching certain position, the salient point 705 on strip touches on collecting chamber side wall quilt after card hole
Release, salient point, which enters, is engaged fixation in card hole.
Test chamber 100
Test chamber 100 is usually a cavity, for accommodating testing element 200, and liquid sample is enable to enter the chamber
Engaged test element is detected in body.Its various shapes, the shape and quantity of the testing element that can be accommodated as needed into
Row design.In the present invention, testing element is test strips, and therefore, in one embodiment, test chamber is plank frame, has bottom plate
102 and upper plate 101, the two forms a cavity 100 after being combined together by way of engaging, more specifically, by test strips
After 200 are placed on bottom plate 102, upper plate 101 is covered, upper plate 101 is fixed on bottom plate 102, forms test chamber 100.
More specifically, there is the strip-shaped clamp groove of one or more protrusions, these card slots are for placing and fixing test strips on bottom plate 102
200.In other limited embodiments, there are one window 103, the detections of window 103 position and test strips for tool on upper plate
Area is corresponding, facilitates the test result of observation test strips.
Sample reaction chamber300
In certain detections, sample needs to be handled in advance before being detected, for example, being diluted, or needs
With a certain reagent react in advance makes analyte therein be activated or labeled etc., therefore sample reaction chamber 300 is storing up
While depositing liquid sample, so that liquid sample is realized in the cavity and mixed with buffer solution, or make a certain reagent and liquid
Body sample carries out pre-reaction so that liquid sample is pretreated in sample reaction chamber.In specific embodiment, sample reaction chamber
200 are enclosed hollow tubular structure by bottom and side wall.
In the following detailed description, the subsidiary reference word of legend is a part here, it is to illustrate this
Invention can the mode of actable specific concrete scheme illustrate.The present invention, which is not precluded, in we can also carry out others specifically
Scheme and the structure for changing the present invention without prejudice to the use scope of the present invention.
First, sample testing apparatus of the invention includes mainly sample reaction chamber 300 and test chamber 100.Wherein, test chamber
100 for disposing testing element 100, while receiving liquid sample, and the liquid sample into test chamber 100 is made sequentially to flow through test
Element 200 realizes detection.And sample reaction chamber 300, for storing liquid sample, and keep liquid sample real in the cavity 300
It is now mixed with buffer solution, or a certain reagent is made to carry out pre-reaction with liquid sample so that liquid sample is reacted in sample
It is pretreated in chamber 300.In order to realize that detection function, test chamber 100 also need to liquid phase flow with sample reaction chamber 300 and lead to, that is,
So that sample is reacted, in the liquid fluid in 300 to test chamber 100 and reaches in test strips 200.Therefore, test chamber 100 with react
A channel is additionally provided between chamber 300.Because liquid sample needs are handled in advance, and not pretreated sample cannot carry out
Detection, so, which can not make reaction chamber 300 be constantly in connected state with test chamber 300, that is to say, that the channel
Closing in the initial state makes test chamber 100 be not attached to lead to 300 liquid of sample reaction chamber;Channel is opened in detecting state to be made
Test chamber 100 and 300 liquid communication of sample reaction chamber.Therefore, how to realize is needing between test chamber 100 and reaction chamber 300
Liquid just circulates as key when detecting.
In the present invention, as shown in Figure 1, test chamber 100 is mutually clamped and is formed by bottom plate 102 and upper plate 101, test strips 200
It is fixed between bottom plate 102 and upper plate 101 in test chamber 100, is corresponded in the top of test strips sample application zone 201
Locate, there are one cavitys 300, the cavity 300 to be enclosed by side wall 305 and bottom 304 for connection on upper plate 101, has open end
303,300 bottom of cavity is also a part for test chamber upper plate 101;The cavity 300 is columnar structured, is vertically positioned at test chamber
On upper plate 101, as shown in Figure 2.It is set there are one through-hole 301 in 300 bottom of reaction chamber, which makes reaction chamber 300 and detection
It is connected between chamber 100, more specifically, through-hole 301 is located at the top of the sample region of acceptance 201 of testing element;In original state
When, through-hole 301 is sealed, and when needing detection, through-hole 301 is made to be made to be connected between reaction chamber 300 and test chamber 100 by unlatching.
The mode that through-hole 301 is sealed is unlimited, that can open when needed, and does not chemically generate shadow to the detection of liquid sample
Loud material can, therefore, it is possible to use diaphragm seal, various sealing-plugs etc., a specific embodiment can also be used
In, through-hole is sealed using through hole plug 310, the material of through hole plug is silica gel.Before sample is detected, through hole plug 310 is located at through-hole
Interior sealing through-hole, the liquid sample in reaction chamber 300 cannot reach test chamber 100, after band sample is pretreated or need to sample
When being originally detected, through-hole 301 is opened, namely removes through hole plug 310 or makes through hole plug 310 by trepanning.Some are specific
In embodiment, in order to easy to operate, through hole plug 310 makes through-hole open by way of being punctured.
More specifically in embodiment, which further includes that a lid closes the lid on sample reaction chamber 300
400, and the raised line 420 that is fixedly connected on lid, through hole plug 310 is poked or punctured by raised line 420.The raised line 420
Be connected to the end 460 of lid 400, when raised line 420 is located in reaction chamber 300, raised line 420 be located at through hole plug 310 just on
Side, in order to make raised line 420 that can poke through hole plug 310 when needed, the length of raised line 420 is slightly larger than or is equal to reaction chamber
300 height.Meanwhile in order to just so that raised line 420 is punctured through hole plug 310 when needed, need raised line 420 in initial position not
Contact through hole plug 310, in one embodiment, also around there are one cyclic annular buckle 600, ring-type buckles 600 on lid 400
In the top of lid 400 450, after the lid of lid 400 is closed to reaction chamber 300, buckle 600 is positioned at the top of lid 450 and reaction chamber
Between 300 accent 303, so that lid top 450 is not contacted with reaction chamber accent 303, as shown in Figure 3 and Figure 4, also make to be connected to
The end of raised line 420 on lid not contact through hole plug 310, as shown in Figure 7.In initial position namely lid 400 is located at the
When one position, buckle 600 is fastened on the raised line 420 at the top of lid between 450 and reaction chamber accent 303, being located in reaction chamber 300
Not contact through hole plug 310;When needing detection, buckle 600 is removed, as shown in figure 5, lid 400 is made to be moved downward to lid top
450 contacting reaction cavity accent 303 of portion, as shown in fig. 6, i.e. lid 400 reaches the second position, during this, with lid 400
The end of the protrusion 420 moved down together contact and puncture through hole plug 310, as shown in Figure 10.It is convex in order to make in some embodiments
Item 420 can accurately be located at the surface of through hole plug 310 so that raised line 420 can smoothly poke through hole plug 310, need solid
Position of the lid 400 on reaction chamber 300 is determined so that the position of raised line 420 is fixed, and lid 600 is fixed by buckle 600, with
Prevent lid 400 from the random change of location on reaction chamber 300, more specifically, the center position of ring-type buckle 600 has
The side wall corresponding position of protrusion 601, lid top 450 has shrinkage pool 401, when buckle protrusion 601 is inserted into covered body wall shrinkage pool
After 401, the position between buckle 600 and lid 400 is fixed to each other.One more specifically in embodiment, in the chamber of reaction chamber
On mouth 303, for also tool there are one groove 302, which is used to place the part that annular buckles 600, more specifically, card
The part for buckling raised 601 junctions is located in the groove 302 of reaction chamber accent.The position of buckle 600 is set to be fixed, even if also
It is fixed with the position for buckling fixed lid 400, so as to realize that the raised line 420 on lid is accurately located in reaction chamber
Through-hole 301 surface.
In order to make the liquid sample in reaction chamber 300 be pretreated, can be added in reaction chamber 300 buffer solution or other
In one embodiment, by reagents loaded on carrier 410, this carrier 410 is pre-placed in reaction chamber 300 for reagent, with
It is conveniently operated.More specifically, there is card slot on lid 400,410 one end of carrier is fixed in card slot, lid is worked as
After 400 lids close reaction chamber, carrier 410 is located in reaction chamber 300.
In some embodiments, it is additionally provided with a stomata 430 on lid 400, reaction chamber 300 is made to be connected with ambient atmosphere
It is logical.
Secondly, sample collection detection device 800 provided by the invention is in addition to the test chamber 100 including detection device, sample
Reaction chamber 300 further includes collection well 500.In one embodiment, test chamber 100 and reaction chamber 300 and collection well
500 are an integral structure.That is, likewise, test chamber 100 is also engaged by bottom plate 102 and upper plate 101, testing element 200
In being fixed in the card slot of bottom plate 102, wherein testing element 200 is multiple, and more specifically, testing element is test strips, the examination
Paper slip is equipped with sample region of acceptance 201, reagent area and detection zone.Sample reaction chamber 300 is to be vertically positioned on upper plate 101, position
In the surface of the sample region of acceptance 201 of test strips 200, reaction chamber 300 is one end (sample receiving terminal) conduct by upper plate 101
Bottom 304, hollow columnar structured made of being enclosed along with side wall 305, open end is accent 303.Reaction chamber 300
Bottom (upper plate 101) is equipped with through-hole 301, as shown in Fig. 2, the sample that the through-hole 301 is located at testing element 200 (test strips) connects
By the top in area 201.When liquid from reaction chamber 300 by through-hole 301 in flow out to test chamber 100 when, liquid contacts survey first
Try the sample region of acceptance 201 of element.As shown in Figure 1, collection well 500 is enclosed by side wall 530 and bottom 540
An one end open 520 cavity, which is located at one end of the upper plate 101 of test chamber 100, one is linked as with upper plate 101
Body, at the same time, collecting chamber 500 link together with reaction chamber 300, specifically, the opening direction of collecting chamber and reaction chamber
Opening direction is mutually perpendicular to, i.e. collecting chamber 500 is laterally positioned on test chamber upper plate 101 namely collecting chamber side wall 530 and test chamber
Upper plate 101 is connected namely the bottom of collecting chamber 500 is connected with the side wall of reaction chamber 300, more specifically, collecting chamber 500
Bottom simultaneously for reaction chamber 300 side wall.In the bottom of collecting chamber 500, make collecting chamber 500 and reaction chamber equipped with through-hole 510
300 connections.
The sample collection detection device 800 further includes sample collection stick 700, and it includes absorption piece 701 to collect stick 700 mainly
With handle 702, the two is connected, and sample is drawn by absorption piece 701, and saliva is drawn for example, being put into subject oral cavity
Sample is affectedly bashful collection stick by handle 702.
Collecting chamber 500 is combined that the liquid sample on absorption piece 701 is made to outflow with collection stick 700, specifically, is made
Absorption piece 701 is inserted into collecting chamber 500, and is moved to 500 base portion of collecting chamber, and absorption piece 701 is made to contact collecting chamber 500
Bottom is simultaneously compressed, and the sample in absorption piece 701, which is extruded, to be passed out, and is flow in collecting chamber 500.Preferably, it is
So that liquid is not outflowed collecting chamber 500, there is the sealing ring 703 of annular in absorption piece 701 and handle 702 junction,
As shown in Figures 2 and 3, which seals the side wall of collecting chamber 500 after collecting stick 700 and being inserted into collecting chamber 500, makes collection
Chamber 500 forms the cavity of a sealing, avoids liquid outflow, meanwhile, with the movement for collecting stick 700, the seal cavity volume
It is gradually reduced, the pressure in seal cavity is made to increase, be more advantageous to extruding absorption piece 701 and let the liquid out.Some embodiments
In, after the absorption piece 701 for collecting stick is all compressed in collecting chamber, collects stick 700 and is locked in collecting chamber 500,
In specific embodiment, there are two symmetrical strip 704, the strips for one end design that handle 702 is connect with absorption piece 701
705 have elasticity, can be by compression and collecting chamber 500.Collecting chamber 500 is symmetrical with 2 on the side wall at accent
Card hole 501, after absorption piece 701 is all compressed, the salient point 705 on strip, which is placed exactly in card hole 501, to be fixed, and receipts are made
Collection stick 700 is locked in collecting chamber 500.
Sample is flowed into after the extrusion of absorption piece 701 in collection well 500 first, then is flowed by through-hole 510
In sample reaction chamber 300.In the sample collection detection device 800 of the present invention, lid 400 is covered on sample reaction chamber 300,
There is stomata 430, the stomata 430 to keep air inside and outside reaction chamber 300 identical, i.e. air pressure in holding reaction chamber 300 is on lid
Atmospheric pressure, in this way, the liquid flowed out after being collected the sealing of stick 700 in collecting chamber 500 and compressing is because air pressure is more than in reaction chamber
Air pressure, make liquid sample pass through through-hole 510 flow into it is more smoothly.There is carrier 410, the carrier in the card slot of lid 400
410 are located in reaction chamber 300, and carrier 410 is loaded with the reagent of pretreatment sample, when liquid sample flows into sample reaction chamber 300
Afterwards, contact carrier 410 is reacted, and realizes pretreatment.There is buckle 600 at 400 top of lid, when the lid with buckle
After 400 lids are closed onto reaction chamber 300, the raised line 420 for being located at lid end is located at the surface of reaction chamber bottom through-hole 301, but
The not through hole plug 310 on contact through hole pushes lid 400 after removing buckle 600, makes 400 top of lid and sample reaction chamber
300 accent contact, during this, raised line 420, which contacts, simultaneously pokes through hole plug 310, makes pretreated in reaction chamber 300
Liquid sample test chamber 100 is flowed by the through hole plug 310 that is punctured, contact, complete with the sample region of acceptance 201 of test strips
Detection.
The collection of sample is carried out with regard to the sample collection detection device 800 of the present invention below and detects analyte in sample
Method is described.
1. in initial position, as shown in Figure 3 and Figure 7, in the sample collection detection device 800 of integral structure, annular
Buckle 600 is separately connected covered body wall shrinkage pool 401 by protrusion 601 and raised junction and reaction chamber groove 302 is fixed, lid
400 are located at first position, at this point, the raised line 420 of lid end is located in reaction chamber 300, right over through hole plug 310, and lead to
Stopple 310 does not contact;It is detached with collecting chamber 500 at this point, collecting stick 700;
2. 701 one end of absorption piece for the collection stick 700 for having liquid sample will be collected towards 500 interpolation of collecting chamber of transverse direction
Enter, after absorption piece 701 fully enters collecting chamber 500, the sealing of sealing ring 703 for being connected to 701 end of absorption piece is collected
Side wall at 500 accent of chamber makes collecting chamber 500 form the cavity of sealing;
3. continuing to promote and collecting stick 700, absorption piece 701 is made to be collected the compression of 500 bottom squeeze of chamber, absorption piece 701
On liquid flow out in collecting chamber 500;Meanwhile reaction chamber 300 is flowed by the through-hole 501 of collecting chamber 500 and reaction chamber 300
It is interior;
4. after absorption piece 701 is all squeezed, the salient point 705 of the strip 704 on handle is engaged to collecting chamber
In the bayonet 501 of side wall, collects stick 700 and be locked in collecting chamber 500, as shown in Figure 4 and Figure 8;
5. making sample collection detection device 800 stand 3-5 minutes, at this point, the liquid sample in reaction chamber 300 and load
Reagent on body 410 is contacted and is reacted, and completes the pretreatment of sample;
6. removing the annular buckle 600 being fastened on lid 400, as shown in figs. 5 and 9;
7. pressing down on lid 400 to close the stomata 430 of lid during pressing;In the process, raised line
420 move with lid 400 to 300 bottom of reaction chamber, come into contact with and puncture through hole plug 310;
8. when the top of lid 400 touches at the accent of reaction chamber 300, stop pressing, at this point, lid 400 is located at
The second position, at this point, through hole plug 310 is punctured, the liquid sample via through holes 301 in reaction chamber 300 enter in test chamber 100
The sample region of acceptance 201 of test strips 200, is detected.