CN111665354B - Shared sample injection microfluidic immunoassay joint inspection device - Google Patents
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Abstract
The invention provides a shared sample injection microfluidic immunoassay joint inspection device, which comprises a joint inspection strip, a washing supply unit and a waste liquid suction and storage unit, wherein the washing supply unit is connected to a sample injection end of the joint inspection strip; the joint inspection strip is concavely provided with a first micro-flow channel and a plurality of second micro-flow channels, the first micro-flow channel extends from a sample inlet end of the joint inspection strip to a sample outlet end of the joint inspection strip, and the second micro-flow channels are intersected and communicated with the first micro-flow channel; a sample storage tank is arranged at the inlet of the first micro-flow channel; marker storage tanks are arranged at intervals of a plurality of second micro-grooves in the first micro-groove channel; and the second micro-flow channels are respectively provided with a project reaction detection groove at one end far away from the first micro-flow channel, and the inner walls of the project reaction detection grooves are respectively and fixedly provided with solid phase components for capturing a plurality of targets or corresponding markers. The device has high detection sensitivity and avoids mutual interference of a plurality of items.
Description
Technical Field
The invention relates to a shared sample injection microfluidic immunoassay joint inspection device, and belongs to the technical field of immunoassay.
Background
In the process of medical diagnosis, a plurality of indexes are needed to be jointly detected for mutual verification to assist in disease diagnosis, so that missing diagnosis or misdiagnosis is avoided, and the accuracy of diagnosis is improved. Meanwhile, the multi-index combined detection is also used for clinically identifying and diagnosing diseases, such as the diagnosis of the investigation of new coronavirus (nCOVI) epidemic caused new coronavirus (COVID-19), and in the diagnosis of the visiting or suspicious population, the examined person should be informed of the infection of the new coronavirus (nCOVI) or not, if not the other pathogen is informed as much as possible, the multi-index combined detection is also needed. Therefore, there is an important real-time need to develop instant diagnostics for multi-index joint detection.
At present, the multi-index joint detection mostly adopts a mark immunity joint detection based on lateral chromatography, and the method generally has two detection methods. The first method is to arrange different detection items on the same nitrocellulose solid phase membrane (NC membrane) in sequence, the markers of different items are stacked together in layers, the samples infiltrate into the marker storage position from top to bottom to combine with the corresponding marked antigen or antibody, and enter the NC membrane through the contact area with the NC membrane, and migrate to the solid phase area through capillary action to combine and deposit with the solid phase component for the second time to form a visible characteristic detection zone, which has the defect that the different items possibly have mutual interference, especially the corresponding antigen and antibody of one molecule can not be detected simultaneously, and the detection index number is limited. The second method is to prepare the different items into independent test strips, and then put them into different slots in the same plastic card respectively, share a sample adding pool or add samples separately, but such test efficiency is low.
Disclosure of Invention
The invention provides a shared sample injection microfluidic immunoassay joint inspection device which can effectively solve the problems.
The invention is realized in the following way:
the shared sample injection microfluidic immunoassay joint inspection device is used for detecting a plurality of target objects and comprises a joint inspection strip, a washing supply unit and a waste liquid suction and storage unit, wherein the washing supply unit is connected to the sample injection end of the joint inspection strip, and the waste liquid suction and storage unit is connected to the sample outlet end of the joint inspection strip;
the joint inspection strip is concavely provided with a first micro-flow channel and a plurality of second micro-flow channels, the first micro-flow channel extends from a sample inlet end of the joint inspection strip to a sample outlet end of the joint inspection strip, and the second micro-flow channels are intersected and communicated with the first micro-flow channel; a sample storage tank is arranged on the first micro-flow channel and close to the sample injection end of the joint inspection strip; a plurality of marker storage tanks are arranged at intervals of the second microsleeps in the first microsleep channel, and markers corresponding to a plurality of targets are respectively arranged in the marker storage tanks; a project reaction detection groove is formed in one end, far away from the first micro-flow channel, of each of the second micro-flow channels, solid phase components for capturing a plurality of targets or corresponding markers are fixedly arranged on the inner wall of each of the project reaction detection grooves, and observation windows are formed in the walls of the joint detection strips corresponding to the bottoms or the sides of the project reaction detection grooves; a film-covered seal is arranged on the upper surface of the joint inspection strip, so that the first micro-flow channel, the plurality of second micro-flow channels, the sample storage tank, the marker storage tank and the project reaction detection tank form a sealed communication cavity; the sample injection end of the joint detection strip extends out of a membrane rupture thorn towards the washing supply unit, and a channel is arranged in the membrane rupture thorn and is communicated with the first micro-flow channel;
the washing supply unit comprises a washing liquid sleeve, a washing liquid pipe and a first sealing ring; the washing liquid pipe is arranged in the washing liquid sleeve, the first sealing ring seals the inlet end of the washing liquid sleeve and is provided with a first hollow matched with the cross section of the sample injection end of the joint detection strip, the sample injection end of the joint detection strip extends into the washing liquid sleeve from the first hollow, the pipe orifice of the washing liquid pipe is coated with a sealing film, and the rupture membrane of the sample injection end of the joint detection strip is opposite to the sealing film; the washing liquid pipe is filled with washing liquid; when the joint inspection strip advances to the washing supply unit, the membrane rupture of the sample injection end of the joint inspection strip pierces the sealing membrane, and the cleaning liquid in the cleaning liquid pipe flows into the first micro-flow channel in the joint inspection strip from the channel pierced by the membrane rupture for washing.
As a further improvement, the waste liquid absorbing and storing unit comprises a waste liquid sleeve, a negative pressure forming valve plate and a second sealing ring; the negative pressure forming valve plate is arranged in the inner cavity of the waste liquid sleeve, the second sealing ring seals the inlet end of the waste liquid sleeve and is provided with a second hollow matched with the cross section shape of the sample outlet end of the joint inspection strip, the center of the negative pressure forming valve plate is provided with a third hollow matched with the cross section shape of the sample outlet end of the joint inspection strip, the sample outlet end of the joint inspection strip extends into the waste liquid sleeve from the second hollow, the negative pressure forming valve plate is fixedly sleeved on the sample outlet end of the joint inspection strip through the third hollow, and the first micro-flow channel is communicated with the inner cavity of the outlet end of the waste liquid sleeve; when the waste liquid absorbing and storing unit slides to the sample outlet end far away from the joint inspection strip, the negative pressure forming valve plate does not move, and negative pressure is generated in the inner cavity of the negative pressure forming valve plate and the outlet end of the waste liquid sleeve to suck the first micro-flow channel.
As a further improvement, the first micro-fluidic channel, the plurality of second micro-fluidic channels, the sample reservoir, the label reservoir, and the item reaction detection reservoir are each surface treated to be hydrophilic.
As a further improvement, the label is selected from colloidal gold, colored microspheres, fluorescent substances, lanthanide rare earth Eu 2+ Any one of quantum dots.
As a further improvement, the joint test strip is integrally molded by compression molding or injection molding.
As a further improvement, the sample reservoir has a larger capacity than the label reservoir.
As a further improvement, a plurality of the second micro-fluidic channels are arranged in parallel and perpendicular to the first micro-fluidic channels.
As a further improvement, a connecting rod is extended from the outer wall of the waste liquid sleeve of the waste liquid absorbing and storing unit along the direction of the joint inspection strip, a chute is arranged on the wall of the washing liquid sleeve of the washing supply unit, and the extending end of the connecting rod is inserted into the chute to clamp the waste liquid absorbing and storing unit and the washing supply unit together.
As a further improvement, the depth of the second micro-fluidic channel is greater than the depth of the first micro-fluidic channel.
The invention provides a use method of the shared sample injection microfluidic immunoassay joint inspection device, which comprises the following steps:
s1, adding sample liquid into a sample storage tank to react;
s2, sliding the waste liquid suction and storage unit to a sample outlet end far away from the joint inspection strip at a speed of 2-5 mm/S;
s3, after the reaction is finished, pushing the joint inspection strip to the washing supply unit until the joint inspection strip feels empty, and at the moment, puncturing the sealing film of the washing liquid pipe by a film puncturing puncture, and washing;
s4, after washing is completed, the detection result is observed or calculated through visual inspection, colorimetric or fluorescent counting at an observation window of the joint detection strip.
The beneficial effects of the invention are as follows:
according to the shared sample injection microfluidic immunoassay joint inspection device, the joint inspection strip is concavely provided with the first micro-flow channel and the plurality of second micro-flow channels, the sample storage tank and the marker storage tank are arranged in the first micro-flow channel, the project reaction detection tank is arranged in the second micro-flow channel, the inner walls of the plurality of project reaction detection tanks are respectively fixedly provided with solid phase components for capturing a plurality of target objects and corresponding markers, when a sample to be inspected is arranged in the sample storage tank, through capillary action, the sample liquid flows along the first micro-flow channel, the target objects in the sample liquid are combined with the corresponding markers through the marker storage tank, then enter the project reaction detection tank in the second micro-flow channel to be combined with the corresponding solid phases, and then observe, record and qualitatively or quantitatively report the results in a visual observation, colorimetric, fluorescent and other modes through the observation window, and the solid phases and the markers are respectively arranged at different positions, so that the mutual interference of the detection of each target object is avoided.
According to the shared sample injection microfluidic immunoassay joint inspection device, sample injection is only needed in the sample storage tank for one time, and the sample liquid can enter the plurality of marker storage tanks and the plurality of item reaction detection tanks through capillary action to detect a plurality of items, so that the detection speed is greatly improved.
According to the shared sample injection microfluidic immunoassay joint inspection device, the washing supply unit is arranged, after reaction detection is completed, the joint inspection strip is pushed to the washing supply unit, the sealing film is pierced by a broken film at the sample injection end of the joint inspection strip, cleaning liquid in the cleaning liquid pipe flows into the joint inspection strip from a channel pierced by the broken film to wash, and residual samples, markers and reaction residues in the shared sample injection microfluidic immunoassay joint inspection device are sufficiently washed, so that interference of the residues on detection is avoided, and the detection sensitivity is remarkably improved.
The shared sample injection microfluidic immunoassay joint inspection device is further provided with the waste liquid suction storage unit, the waste liquid suction storage unit is internally provided with the negative pressure valve plate, when the waste liquid suction storage unit slides to the sample outlet end far away from the joint inspection strip, the negative pressure forming valve plate does not move, negative pressure is generated in the inner cavity of the negative pressure forming valve plate and the outlet end of the waste liquid sleeve, the negative pressure can accelerate the flow of liquid in the first micro-flow channel and the second micro-flow channel, the reaction speed is improved, and the detection speed is further improved.
The shared sample injection microfluidic immunoassay joint inspection device has the advantages of simple structure, easy operation, meeting various application scenes and reducing medical detection cost.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some examples of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
Fig. 1 is a schematic structural diagram of an integral shared sample injection microfluidic immunoassay joint inspection device according to an embodiment of the present invention.
Fig. 2 is a schematic top view of a joint inspection strip of the shared sample injection microfluidic immunoassay joint inspection device according to an embodiment of the present invention.
Fig. 3 is a schematic front view of a joint detection strip of the shared sample injection microfluidic immunoassay joint detection device according to the embodiment of the present invention.
Reference numerals:
a joint detection strip 1, a washing supply unit 2, a first micro-flow channel 11, a second micro-flow channel 12, a sample storage tank 13, a marker storage tank 14, a project reaction detection tank 15, a coating film 16, a film breaking thorn 17,
a washing supply unit 2, a washing liquid sleeve 21, a washing liquid pipe 22, a first sealing ring 23, a sealing film 24 and a chute 25,
the waste liquid absorbing and storing unit 3, the waste liquid sleeve 31, the negative pressure forming valve plate 32, the second sealing ring 33 and the connecting rod 34.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention. Thus, the following detailed description of the embodiments of the invention, as presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, based on the embodiments of the invention, which are apparent to those of ordinary skill in the art without inventive faculty, are intended to be within the scope of the invention.
In the description of the present invention, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
Referring to fig. 1 to 3, the present invention provides a shared sample injection microfluidic immunoassay joint inspection device, which is used for detecting a plurality of target objects, and comprises a joint inspection strip 1, a washing supply unit 2 and a waste liquid suction storage unit 3, wherein the washing supply unit 2 is connected to a sample injection end of the joint inspection strip 1, and the waste liquid suction storage unit 3 is connected to a sample discharge end of the joint inspection strip. The sample liquid inlet end of the joint detection strip 1 is a sample inlet end, and the sample liquid outlet end is a sample outlet end.
The joint inspection strip 1 is concavely provided with a first micro-flow channel 11 and a plurality of second micro-flow channels 12, the first micro-flow channel 11 extends from the sample inlet end of the joint inspection strip 1 to the sample outlet end of the joint inspection strip 1, and the second micro-flow channels 12 are intersected and communicated with the first micro-flow channel 11.
The first micro-fluidic channel 11 is provided with a sample reservoir 13 near the sample inlet end of the joint detection strip 1. The first micro-flow channel 11 is provided with a marker storage tank 14 at the interval of the second micro-flow channels 12, and markers corresponding to the targets are respectively arranged in the marker storage tanks 14. The second micro-fluidic channels 12 are each provided with a project reaction detection channel 15 at one end far away from the first micro-fluidic channel 11, and solid phase components for capturing a plurality of targets or corresponding markers are fixedly arranged on the inner walls of the project reaction detection channels 15. A plurality of the marker storage tanks 14 from the sample inlet end to the sample outlet end of the joint detection strip 1 are respectively provided with a first marker and second markers to Nth markers corresponding to the first target and the second target to the Nth target; the solid phase components in the project reaction detection grooves 15 from the sample inlet end to the sample outlet end of the joint detection strip 1 are respectively a first solid phase component and a second solid phase component to an nth solid phase component (N is a natural number and is equal to or more than 3) corresponding to the first target object and the second target object to the nth target object. In this embodiment, n=7 is preferable. In the device, the markers and the corresponding solid phase components are independently arranged at different positions, and the solid phase components are also mutually independent, so that the mutual interference of detection items is avoided, and the detection sensitivity is improved. The target may be a protein antigen or antibody, hapten T3, T4, etc., and the solid phase is divided into an antibody, antigen, anti-T3 antibody, anti-T4 antibody, etc., which may be an anti-protein antigen.
An observation window is provided in the wall of the joint detection strip corresponding to the bottom or side surface of the plurality of item reaction detection grooves 15. When visually observed, the bottom can be seen directly above, or the side wall can be seen laterally; colorimetric detection is performed by using the side wall; when fluorescence detection is used, the side wall and the bottom can be excited directly above, and the side wall detects fluorescence; the side wall may also excite the side wall and bottom, the upper portion detecting fluorescence. Visual and instrumental detection results may be used to report results qualitatively or quantitatively.
The upper surface of the joint inspection strip 1 is provided with a coating film 16 for sealing, so that the first micro-flow channel 11, the second micro-flow channels 12, the sample storage tank 13, the marker storage tank 14 and the project reaction detection tank 15 form a sealed communication cavity, the formation of capillary action and the formation of negative pressure by the subsequent waste liquid suction and storage unit 3 are facilitated, the liquid flow rate is accelerated, and the detection speed and efficiency are improved.
The sample injection end of the joint inspection strip 1 extends out of a membrane rupture thorn 17 towards the washing supply unit 2, and a channel is arranged in the membrane rupture thorn 17 and communicated with the first micro-flow channel 11.
The washing supply unit 2 includes a washing liquid casing 21, a washing liquid pipe 22, and a first gasket 23; the washing liquid pipe 22 is arranged in the washing liquid sleeve 21, the first sealing ring 23 seals the inlet section of the washing liquid sleeve 21 and is provided with a first hollow matched with the cross section shape of the sample injection end of the joint inspection strip 1, the sample injection end of the joint inspection strip 1 extends into the washing liquid sleeve 21 from the first hollow, the pipe orifice of the washing liquid pipe 22 is coated with a sealing film 24, and the film breaking thorn 17 of the sample injection end of the joint inspection strip 1 is opposite to the sealing film 24; the wash pipe 22 contains a wash liquid. The washing liquid sleeve 21 is a round barrel structure integrated by injection molding, and the first sealing ring 23 is a ring structure made of rubber or silica gel.
When the joint inspection strip 1 is pushed to the washing supply unit 2, the membrane breaking thorn 17 at the sample injection end of the joint inspection strip 1 pierces the sealing membrane 24, and the cleaning liquid in the cleaning liquid pipe 22 flows into the first micro-groove channel 11 in the joint inspection strip 1 from the channel of the membrane breaking thorn 17 for washing.
The waste liquid suction and storage unit 3 comprises a waste liquid sleeve 31, a negative pressure forming valve plate 32 and a second sealing ring 33. One end of the waste liquid sleeve 31, which is close to the joint detection strip 1, is defined as an inlet end, and the other end is defined as an outlet end. The negative pressure forming valve plate 32 is arranged in the inner cavity of the waste liquid sleeve 31, the second sealing ring 33 seals the inlet end of the waste liquid sleeve 31 and is provided with a second hollow matched with the cross section shape of the sample outlet end of the joint inspection strip 1, the center of the negative pressure forming valve plate 32 is provided with a third hollow matched with the cross section shape of the sample outlet end of the joint inspection strip 1, the sample outlet end of the joint inspection strip 1 extends into the waste liquid sleeve 31 from the second hollow, the negative pressure forming valve plate 32 is fixedly sleeved on the sample outlet end of the joint inspection strip 1 through the third hollow, and the first micro-flow channel 11 is communicated with the inner cavity of the outlet end of the waste liquid sleeve 31; when the waste liquid absorbing and storing unit 3 slides to the sample outlet end far away from the joint inspection strip 1, the negative pressure forming valve plate 32 does not move, negative pressure is generated in the inner cavity of the outlet ends of the negative pressure forming valve plate 32 and the waste liquid sleeve 31, the first micro-flow channel 11 is pumped, the flow of liquid in the first micro-flow channel 11, the second micro-flow channels 12, the sample storage tank 13, the marker storage tank 14 and the project reaction detection groove 15 is quickened, and the reaction speed and the detection efficiency are improved.
The waste liquid sleeve 31 is of an injection-molded integrated barrel structure, and the second sealing ring 33 is of a ring structure made of rubber or silica gel.
The first micro-fluidic channel 11, the second micro-fluidic channels 12, the sample reservoir 13, the label reservoir 14, and the item reaction detection reservoir 15 are all surface treated to be hydrophilic, so as to facilitate capillary action to promote liquid flow.
The marker is selected from colloidal gold, colored microspheres, fluorescent substances and lanthanide rare earth Eu 2+ Any one of quantum dots. The device is suitable for detection by various detection means.
The joint inspection strip 1 is integrally formed by compression molding or injection molding of polymer or silicate glass, the first micro-flow channel 11, the plurality of second micro-flow channels 12, the sample storage tank 13, the marker storage tank 14 and the project reaction detection tank 15 are all grooves formed by recessing the upper surface of the joint inspection strip 1, and the grooves are covered with a coating film 16 to form sealed slit cavities, and have hydrophilicity so as to facilitate capillary action to promote liquid flow.
The sample reservoir 13 has a capacity greater than the label reservoir 14, preferably the sample reservoir 13 has a capacity 10-20 times the capacity of the label reservoir 14. In this range, a large amount of sample liquid can be added to the sample storage tank 13 at a time, and the sample liquid can fill all of the marker storage tank 14 and the item reaction detection tank 15, so that a plurality of items can be detected by one sample addition. If the multiple is more than 20, the volume of the sample reservoir 13 is excessively large, and it is difficult to form capillary action, which is disadvantageous for the flow of liquid.
Preferably, the second micro-flow channels 12 are arranged in parallel and perpendicular to the first micro-flow channels 11, the first micro-flow channels 11 pass through midpoints of lengths of the second micro-flow channels 12, and the project reaction detection grooves 15 can be formed at two ends of the second micro-flow channels 12, so that detection reactions can be observed from two sides of the joint detection strip, and two sides can realize simultaneous detection by different detection means, thereby realizing more efficient detection.
A connecting rod 34 extends from the outer wall of the waste liquid sleeve 31 of the waste liquid absorbing and storing unit 3 along the direction of the joint detection strip 1, a chute 25 is arranged on the wall of the washing liquid sleeve 21 of the washing supply unit 2, and the extending end of the connecting rod 34 is inserted into the chute 25 to realize clamping connection. The waste liquid absorbing and storing unit 3, the joint inspection strip 1 and the washing supply unit 2 are connected and fixed into a whole through the connecting rod 34, and the waste liquid sleeve 31 and the washing liquid sleeve 21 are cylindrical, so that the whole shared sample injection microfluidic immunoassay joint inspection device forms a pen-like structure and is easy to carry.
The length of the second micro flow channels 12 is 10-15mm, the distance between the intervals between the plurality of second micro flow channels 12 is 10-15mm, the length of the first micro flow channels 11 can be adjusted according to the number of the second micro flow channels 12, the number of the second micro flow channels 12 is preferably 3-10, and the length of the first micro flow channels 11 is preferably 40-200mm. The width of the first and second micro-fluidic channels 11, 12 is 1.5-2.5mm, preferably 2mm. The depth of the second micro flow channel 12 is greater than the depth of the first micro flow channel 11, preferably the depth of the second micro flow channel 12 is 2.0-2.5mm, and the depth of the first micro flow channel 11 is 1.0-1.5mm. Under the dimensions of the length and the depth, a narrow cavity is formed, capillary action is facilitated, liquid can be fully distributed, meanwhile, the flow of the liquid is quickened, and the detection efficiency is improved. The depth of the second micro-fluidic channel 12 is greater than that of the first micro-fluidic channel 11, so that the reacted target and the label can stay in the second micro-fluidic channel 12 for a longer time, fully react with the corresponding solid phase component, fully capture the solid phase component, and improve the accuracy and the sensitivity of detection.
The liquid outlet end of the marker storage tank 14 is lower than the liquid inlet end, so that liquid can flow into the marker storage tank 14.
The sample storage tank 13 and the marker storage tank 14 are both elliptic, the elliptic shape is smaller than the square shape, the water caltrop is less, the liquid flow is smoother, no dead angle is left in the washing, and the detection sensitivity is further improved.
The prepared shared sample injection microfluidic immunoassay joint inspection device is stored in a sealing bag in a sealing way.
The application method of the shared sample injection microfluidic immunoassay joint inspection device comprises the following steps:
opening a sealing bag of the shared sample injection microfluidic immunoassay joint inspection device, adding a sample liquid such as blood into the sample storage tank 11, enabling the sample liquid to flow into the marker storage tank 14 along the first micro-fluidic channel 11 under the capillary action, combining a first target object in the sample liquid with a first marker in the marker storage tank 14 to form a first reaction chain mixture, enabling the first reaction chain mixture to continuously flow in the first micro-fluidic channel 11 and the second micro-fluidic channel 12 under the capillary action, and enabling the first reaction chain mixture to flow into the project reaction detection tank, fully react with a corresponding first target object solid phase component and be fixed on the solid phase component. And thus, the detection reaction is completed until the second object to the Nth object are detected.
In the reaction process, the waste liquid absorbing and storing unit 3 slides at a speed of 2-5mm/s far away from the sample outlet end of the joint detection strip 1, the negative pressure forming valve plate 32 is fixed on the sample outlet end of the joint detection strip 1 and does not move, negative pressure is generated in the inner cavities of the negative pressure forming valve plate 32 and the outlet end of the waste liquid sleeve 31, and the liquid in the first micro-flow channel 11, the plurality of second micro-flow channels 12, the sample storage tank 13, the marker storage tank 14 and the project reaction detection groove 15 is pumped into the inner cavity of the waste liquid sleeve 31, so that the flow of the liquid is accelerated, and the reaction proceeding and the detection speed are accelerated. The sliding speed is very critical, and if the sliding speed is smaller than 2mm/s, the negative pressure is too small, so that the reaction speed is difficult to accelerate; if the sliding speed is greater than 5mm/s, the negative pressure is too high, the suction is too high, the reaction of the target, the marker and the solid phase component is insufficient, and the detection sensitivity is lowered.
After the reaction is completed, the joint inspection strip 1 is held and pushed to the washing supply unit 2 until a falling feeling is felt, at this time, the sealing film 24 of the washing liquid pipe 22 is pierced by the film breaking thorn 17, and the washing liquid in the washing liquid pipe 22 flows into the first micro-fluidic channel 11 in the joint inspection strip 1 to wash under the capillary action from the channel of the film breaking thorn 17, and the first micro-fluidic channel 11, the plurality of second micro-fluidic channels 12, the sample storage tank 13, the marker storage tank 14 and the project reaction detection tank 15 are washed in sequence. Residual sample liquid, labels, etc. are washed away, and the reaction chain mixture immobilized on the solid phase component is not washed away. In the washing process, the waste liquid absorbing and storing unit 3 can also slide at a speed of 6-10mm/s towards the sample outlet end far away from the joint inspection strip 1, and the cleaning liquid and the residual liquid are pumped into the inner cavity of the waste liquid sleeve 31, so that the washing process is quickened. The sliding speed is very critical, and if the sliding speed is less than 6mm/s, the sliding speed is difficult to clean, and if the sliding speed is more than 10mm/s, the suction force is too high, so that the solid phase component is easily extracted, and the detection sensitivity is affected.
After washing, the detection result can be observed and calculated through visual inspection, colorimetric or fluorescent counting at the observation window of the joint detection strip 1, so that interference of residues on detection is avoided, and the detection sensitivity is greatly improved. When in visual observation, the bottom can be seen directly above, or the side wall can be seen laterally; colorimetric detection is performed by using the side wall; when fluorescence detection is used, the side wall and the bottom can be excited directly above, and the side wall detects fluorescence; the side wall may also excite the side wall and bottom, the upper portion detecting fluorescence. Visual and instrumental detection results may be used to report results qualitatively or quantitatively.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and various modifications and variations may be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (9)
1. The shared sample injection microfluidic immunoassay joint inspection device is used for detecting a plurality of target objects and is characterized by comprising a joint inspection strip, a washing supply unit and a waste liquid suction and storage unit, wherein the washing supply unit is connected to the sample injection end of the joint inspection strip, and the waste liquid suction and storage unit is connected to the sample outlet end of the joint inspection strip;
the joint inspection strip is concavely provided with a first micro-flow channel and a plurality of second micro-flow channels, the first micro-flow channel extends from a sample inlet end of the joint inspection strip to a sample outlet end of the joint inspection strip, and the second micro-flow channels are intersected and communicated with the first micro-flow channel; a sample storage tank is arranged on the first micro-flow channel and close to the sample injection end of the joint inspection strip; a plurality of marker storage tanks are arranged at intervals of the second microsleeps in the first microsleep channel, and markers corresponding to a plurality of targets are respectively arranged in the marker storage tanks; a project reaction detection groove is formed in one end, far away from the first micro-flow channel, of each of the second micro-flow channels, solid phase components for capturing a plurality of targets or corresponding markers are fixedly arranged on the inner wall of each of the project reaction detection grooves, and observation windows are formed in the walls of the joint detection strips corresponding to the bottoms or the sides of the project reaction detection grooves; a film-covered seal is arranged on the upper surface of the joint inspection strip, so that the first micro-flow channel, the plurality of second micro-flow channels, the sample storage tank, the marker storage tank and the project reaction detection tank form a sealed communication cavity; the sample injection end of the joint detection strip extends out of a membrane rupture thorn towards the washing supply unit, and a channel is arranged in the membrane rupture thorn and is communicated with the first micro-flow channel; the capacity of the sample storage tank is 10-20 times of the capacity of the marker storage tank; the two membrane breaking thorns are arranged, one ends of the two membrane breaking thorns are connected to two sides of the sample injection end of the joint inspection strip, the other ends of the two membrane breaking thorns are close to each other and a gap is reserved, and the gap forms a channel;
the washing supply unit comprises a washing liquid sleeve, a washing liquid pipe and a first sealing ring; the washing liquid pipe is arranged in the washing liquid sleeve, the first sealing ring seals the inlet end of the washing liquid sleeve and is provided with a first hollow matched with the cross section of the sample injection end of the joint detection strip, the sample injection end of the joint detection strip extends into the washing liquid sleeve from the first hollow, the pipe orifice of the washing liquid pipe is coated with a sealing film, and the rupture membrane of the sample injection end of the joint detection strip is opposite to the sealing film; the washing liquid pipe is filled with washing liquid; when the joint inspection strip is pushed to the washing supply unit, a membrane rupture at the sample injection end of the joint inspection strip pierces the sealing membrane, and the cleaning liquid in the cleaning liquid pipe flows into a first micro-flow channel in the joint inspection strip from a channel pierced by the membrane rupture for washing;
the waste liquid absorbing and storing unit comprises a waste liquid sleeve, a negative pressure forming valve plate and a second sealing ring; the negative pressure forming valve plate is arranged in the inner cavity of the waste liquid sleeve, the second sealing ring seals the inlet end of the waste liquid sleeve and is provided with a second hollow matched with the cross section shape of the sample outlet end of the joint inspection strip, the center of the negative pressure forming valve plate is provided with a third hollow matched with the cross section shape of the sample outlet end of the joint inspection strip, the sample outlet end of the joint inspection strip extends into the waste liquid sleeve from the second hollow, the negative pressure forming valve plate is fixedly sleeved on the sample outlet end of the joint inspection strip through the third hollow, and the first micro-flow channel is communicated with the inner cavity of the outlet end of the waste liquid sleeve; when the waste liquid absorbing and storing unit slides to a sample outlet end far away from the joint inspection strip, the negative pressure forming valve plate does not move, and negative pressure is generated in the inner cavity of the negative pressure forming valve plate and the outlet end of the waste liquid sleeve to suck the first micro-flow channel.
2. The shared sample injection microfluidic immunoassay joint inspection device according to claim 1, wherein the first microfluidic channel, the plurality of second microfluidic channels, the sample storage tank, the marker storage tank and the item reaction detection tank are all surface treated to be hydrophilic.
3. The shared sample injection microfluidic immunoassay joint inspection device according to claim 1, wherein the marker is selected from the group consisting of colloidal gold, colored microspheres, fluorescent substances, lanthanide rare earth element Eu 2+ Any one of quantum dots.
4. The shared sample injection microfluidic immunoassay joint inspection device according to claim 1, wherein the joint inspection strip is integrally formed by compression molding or injection molding.
5. The shared sample microfluidic immunoassay joint inspection device of claim 1, wherein the sample reservoir has a greater capacity than the marker reservoir.
6. The shared sample injection microfluidic immunoassay joint inspection device according to claim 1, wherein a plurality of the second microfluidic channels are arranged in parallel and perpendicular to the first microfluidic channels.
7. The shared sample injection microfluidic immunoassay joint inspection device according to claim 2, wherein a connecting rod extends out of the outer wall of the waste liquid sleeve of the waste liquid suction and storage unit along the joint inspection strip, a chute is arranged on the wall of the washing liquid sleeve of the washing supply unit, and the extending end of the connecting rod is inserted into the chute to clamp the waste liquid suction and storage unit and the washing supply unit together.
8. The shared sample injection microfluidic immunoassay joint inspection device of claim 1, wherein the depth of the second microfluidic channel is greater than the depth of the first microfluidic channel.
9. A method of using the shared sample microfluidic immunoassay joint inspection device of any of claims 1 to 8, comprising the steps of:
s1, adding sample liquid into a sample storage tank to react;
s2, sliding the waste liquid suction and storage unit to a sample outlet end far away from the joint inspection strip at a speed of 2-5 mm/S;
s3, after the reaction is finished, pushing the joint inspection strip to the washing supply unit until the joint inspection strip feels empty, and at the moment, puncturing the sealing film of the washing liquid pipe by a film puncturing puncture, and washing;
s4, after washing is finished, observing or calculating a detection result through visual inspection, colorimetric or fluorescent counting in an observation window of the joint detection strip;
during the washing process, the waste liquid absorbing and storing unit slides at a speed of 6-10mm/s towards the sample outlet end of the joint detection strip.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448716A (en) * | 2002-03-28 | 2003-10-15 | 富士胶片株式会社 | Humoral testing unit |
| CN108704680A (en) * | 2018-05-24 | 2018-10-26 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
| CN109975563A (en) * | 2019-03-21 | 2019-07-05 | 厦门先明生物技术有限公司 | A kind of integrated reagent device of microfluid immunoassay |
| CN110554184A (en) * | 2019-09-12 | 2019-12-10 | 深圳市科瑞达生物技术有限公司 | Chemiluminescent microfluidic chip |
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| CN108686721B (en) * | 2017-04-06 | 2021-04-20 | 美康生物科技股份有限公司 | Micro-fluidic chip for separating and detecting whole blood sample and detection method thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1448716A (en) * | 2002-03-28 | 2003-10-15 | 富士胶片株式会社 | Humoral testing unit |
| CN108704680A (en) * | 2018-05-24 | 2018-10-26 | 深圳市帝迈生物技术有限公司 | A kind of micro-fluidic chip and immunofluorescence analysis instrument |
| CN109975563A (en) * | 2019-03-21 | 2019-07-05 | 厦门先明生物技术有限公司 | A kind of integrated reagent device of microfluid immunoassay |
| CN110554184A (en) * | 2019-09-12 | 2019-12-10 | 深圳市科瑞达生物技术有限公司 | Chemiluminescent microfluidic chip |
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