CN211043403U

CN211043403U - Single-channel push irrigation type micro-fluidic chip

Info

Publication number: CN211043403U
Application number: CN201921577404.1U
Authority: CN
Inventors: 唐勇; 廖政
Original assignee: Sichuan Weikang Park Lan Medical Technology Co ltd
Current assignee: Sichuan Weikang Park Lan Medical Technology Co ltd
Priority date: 2019-09-20
Filing date: 2019-09-20
Publication date: 2020-07-17
Anticipated expiration: 2029-09-20

Abstract

The utility model discloses a single channel pushes away irritates formula micro-fluidic chip relates to biological detection technical field. The micro-flow channel comprises a sample adding port for injecting a sample, a reaction bin and a waste liquid bin which are mutually communicated, and a first vent hole positioned on the side wall of the waste liquid bin, wherein a time delay channel for the sample to flow is arranged between the reaction bin and the waste liquid bin; a closed liquid storage bin for containing cleaning liquid is also arranged in the chip body, one end of the liquid storage bin is communicated with one end, close to the reaction bin, of the delay channel through an injection channel, and the other end of the liquid storage bin is provided with an opening; the device also comprises a pushing assembly which can be slidably sealed at the opening of the liquid storage bin and a blocking piece which is used for closing the injection channel. So set up, can adjust and control the injection quantity and the injection speed that the washing liquid pours into the microchannel to effectively prevent in the bubble gets into the microchannel.

Description

Single-channel push irrigation type micro-fluidic chip

Technical Field

The utility model relates to a biological detection technical field, more specifically say, relate to a single channel pushes away irritates formula micro-flow control chip.

Background

The microfluidic chip technology integrates basic operation units of sample preparation, reaction, separation, detection and the like in biological, chemical and medical analysis processes into a micron-scale chip, and automatically completes the whole analysis process. Due to its great potential in the fields of biology, chemistry, medicine and the like, the method has been developed into a new research field crossing the disciplines of biology, chemistry, medicine, fluid, electronics, materials, machinery and the like. At present, microfluidic chips have been applied in the field of immunoassay. Immunofluorescence techniques are commonly used in the field of immunoassay.

The immunofluorescence technique is that according to the principle of antigen-antibody reaction, known antigen or antibody is coated with fluorescent markers such as fluorescein or fluorescent microspheres, and then the antibody/antigen coated with the fluorescent markers is used as a molecular probe to enter a detection sample to be combined with corresponding antigen (or antibody) in detection cells or tissues in the sample to form a combination. The conjugate formed in the cell or tissue contains a fluorescent marker, and the fluorescent marker is irradiated by excitation light (such as a fluorescence microscope), so that the fluorescent marker emits bright fluorescence under the irradiation of the excitation light, a detection person can see the cell or tissue where the fluorescence is located, the nature and the location of the antigen or antibody can be determined, and the fluorescence intensity of the conjugate can be measured by a quantitative detection technology to judge parameters such as the concentration of a sample to be detected.

In the prior art, a micro-fluidic chip applied to an immunofluorescence technique is to arrange a micro-channel structure and other functional elements on the chip, wherein an antibody/antigen coated with a fluorescent marker and a capture antibody/antigen are fixed in the micro-channel. The detection sample is added into the micro-flow channel, and flows through the antibody/antigen coated with the fluorescent marker and the capture antibody/antigen to be subjected to immunoreaction with the antibody/antigen and the capture antibody/antigen to generate a conjugate containing the fluorescent marker and the capture antibody/antigen. The conjugate can be captured and fixed in the micro-flow channel, then cleaning fluid is added into the micro-flow channel through an injection channel communicated with the micro-flow channel to wash away the detection sample except the conjugate, so that only the conjugate remains in the micro-flow channel, and then the conjugate can be detected to obtain various parameters of the detection sample. Although the technology enables the mixing, reaction and separation of the sample and the fluorescent marker to be carried out in one device, the full-automatic micro-fluidic detector which is manually or is matched with the micro-fluidic chip is required to take the cleaning fluid out of the cleaning fluid packaging bottle and add the cleaning fluid into the micro-channel, the operation is not simple enough, and the full-automatic micro-fluidic detector has a complex structure and high production cost; when the cleaning liquid is injected into the micro-channel, the cleaning liquid carries air, and the air enters the micro-channel to form bubbles which influence the detection result of the detector on the combination; the speed of the cleaning liquid injected into the micro-channel can not be regulated, when the speed is too slow, the time for the cleaning liquid to be injected into the micro-channel and completely flow out is too long, more bubbles are easily generated, and when the speed is too fast, the cleaning liquid is easy to wash away the conjugate, so that the error of the detection result is larger.

In view of the above, how to solve the problems that when a cleaning solution is injected into a microfluidic chip, bubbles are contained in a microchannel and the injection speed of the cleaning solution is not easy to control becomes a problem that needs to be solved by those skilled in the art.

SUMMERY OF THE UTILITY MODEL

The utility model provides a single channel pushes away irritates formula micro-flow control chip, it encapsulates the washing liquid in the chip and can adjust and control the injection volume and the injection velocity that the washing liquid pours into the miniflow into in the miniflow way, and in the washing liquid got into the miniflow way through the mode of sealing the circulation, effectively prevented in the bubble got into the miniflow way.

The utility model provides a single-channel push-filling type microfluidic control chip, which comprises a chip body, wherein a closed type microchannel for sample flowing is arranged on the chip body, the microchannel comprises a sample adding port for sample injection, a reaction bin and a waste liquid bin which are mutually communicated, and a first air vent positioned on the top wall of the waste liquid bin, and a delay channel for sample flowing is arranged between the reaction bin and the waste liquid bin; the chip body is also internally provided with a closed liquid storage bin for containing cleaning liquid, one end of the liquid storage bin is communicated with one end, close to the reaction bin, of the delay channel through an injection channel, the other end of the liquid storage bin is provided with an opening, and the single-channel push-irrigation type microfluidic chip further comprises a pushing assembly and a blocking piece, wherein the pushing assembly is slidably sealed at the opening of the liquid storage bin, and the blocking piece is used for closing the injection channel.

Preferably, the pushing assembly comprises a piston which is positioned in the liquid storage bin and is matched with the cross section of the liquid storage bin, and a pushing rod which extends into the opening and is used for pushing the piston to slide along the length direction of the liquid storage bin, and the length of the pushing rod is greater than that of the liquid storage bin.

Preferably, a sealing film for sealing the opening of the liquid storage bin is arranged at the opening of the liquid storage bin.

Preferably, the barrier is arranged to be arranged in the liquid storage bin and used for wrapping the film bag of the cleaning liquid.

Preferably, the liquid storage bin is provided with a puncture hole for the puncture needle to extend into, and one end of the puncture needle, which is far away from the needle tip, is provided with a sealing plug for sealing the puncture hole.

Preferably, the reaction device further comprises a second communication hole which is located on the top wall of the reaction bin and is communicated with the reaction bin, the bottom walls of the reaction bin and the delay channel are flush, the distance between the top wall of the reaction bin and the bottom wall of the reaction bin is larger than the distance between the top wall of the delay channel and the bottom wall of the delay channel, and the cross section size of the delay channel is smaller than that of the reaction bin.

Preferably, the chip body comprises a substrate provided with a cavity and an upper cover connected with the substrate, wherein the cavity is sealed by the upper cover, and the micro-channel is formed.

Preferably, the liquid storage bin is located on the upper cover and protrudes out of the lower surface of the upper cover, the liquid storage bin is provided with a communication port through which the cleaning liquid flows out, the substrate is provided with a notch for accommodating the liquid storage bin and an injection channel for communicating the communication port with the delay channel, and the puncture hole is aligned with the communication port.

Preferably, the micro flow channel further comprises a sample adding bin communicated with the reaction bin and a capturing bin positioned between the delay channel and the waste liquid bin, the sample adding port is positioned in the sample adding bin, a solid reagent ball which has an immunoreaction with the sample is arranged in the sample adding bin, the reagent ball comprises an antibody/antigen coated with a fluorescent marker and a magnetic bead, and a magnet for adsorbing a conjugate generated by the immunoreaction with the antibody/antigen is arranged on the bottom wall of the capturing bin.

Preferably, a communication channel is arranged between the sample adding bin and the reaction bin; the cross-sectional area of the communication channel is gradually reduced along the direction from the sample loading bin to the reaction bin; the delay channel is provided with a plurality of capillary flow channels, the capillary flow channels are arranged along the direction from the sample adding bin to the capturing bin, and the capillary flow channels are sequentially communicated end to form the delay channel in an S-shaped structure.

The utility model provides an among the technical scheme, be provided with closed miniflow channel on the single channel pushes away irritates formula microfluidic control chip's the chip body, flow for the sample. The micro flow channel is provided with a sample adding port communicated with the outside atmosphere, a reaction bin, a waste liquid bin and a first vent hole, wherein the reaction bin and the waste liquid bin are communicated with each other, and the first vent hole is positioned in the waste liquid bin, so that a sample can normally flow, can carry out immunoreaction and flows into the waste liquid bin after the immunoreaction is completed. A delay channel for sample circulation is arranged between the reaction bin and the waste liquid bin so as to ensure the time required by the sample to carry out immunoreaction. Simultaneously, still be provided with the closed stock solution storehouse that is used for holding the washing liquid on the chip body, stock solution storehouse one end is provided with the opening through the one end intercommunication, the other end that are close to the reaction bin of filling into passageway and time delay passageway. And the injection channel is provided with a blocking piece which can seal the injection channel, and the opening of the liquid storage bin is connected with a pushing assembly which can seal the opening and can slide along the length direction or the width direction of the liquid storage bin. When cleaning liquid is not required to be injected into the micro-channel, the injection channel can be sealed through the separation piece, and the cleaning liquid is sealed in the liquid storage bin; when the cleaning liquid needs to be injected, the blocking piece is removed or moved, the injection channel is conducted, and the pushing assembly is pushed to push the cleaning liquid in the liquid storage bin into the injection channel so that the cleaning liquid flows into the micro-channel. Due to the arrangement, the liquid storage bin is closed, the cleaning liquid can be prevented from contacting with the external atmosphere by pushing and injecting the cleaning liquid, and air brought by the cleaning liquid during injection is prevented from forming bubbles in the micro-channel; the pushing speed can be adjusted to control the injection speed of the cleaning liquid so as to avoid the problem of over-high speed or over-low speed; the injection amount of the cleaning liquid into the micro flow channel can also be adjusted.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic diagram of an overall composition structure of a single-channel push-irrigation type microfluidic chip according to an embodiment of the present invention;

fig. 2 is a cross-sectional view of a single-channel push-irrigation type microfluidic chip according to an embodiment of the present invention;

FIG. 3 is a schematic structural diagram of a substrate according to an embodiment of the present invention;

FIG. 4 is a schematic view of the lower surface of the upper cover in the embodiment of the present invention;

fig. 5 is an external schematic view of a single-channel push-irrigation type microfluidic chip according to an embodiment of the present invention.

In fig. 1-5:

the device comprises an upper cover-1, a substrate-2, a micro-flow channel-3, a reaction chamber-301, a delay channel-302, a waste liquid chamber-303, a sample adding chamber-304, a sample adding port-305, a second vent hole-306, a capture chamber-307, a communication channel-308, a liquid storage chamber-4, an opening-401, a piston-402, a push rod-403, a sealing membrane-404, a puncture hole-405, an injection channel-5 and a puncture needle-6.

Detailed Description

In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. It is to be understood that the embodiments described are only some embodiments of the invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.

The objective of the present embodiment is to provide a single-channel push-filling type microfluidic chip, which encapsulates a cleaning solution in the single-channel push-filling type microfluidic chip and can adjust and control the injection speed and injection amount of the cleaning solution into a microchannel, and the cleaning solution enters the microchannel in a closed circulation manner, thereby effectively preventing bubbles from entering the microchannel.

Hereinafter, embodiments will be described in detail with reference to the accompanying drawings. The embodiments described below do not limit the scope of the invention described in the claims. Further, the entire contents of the configurations shown in the following embodiments are not limited to those necessary as a solution of the invention described in the claims.

Referring to fig. 1-5, in the single-channel push-and-flow microfluidic chip provided in this embodiment, a closed microfluidic channel 3 is disposed on a chip body for sample flowing, and enabling the sample to generate immune reaction and sample separation during the flowing process. The micro flow channel 3 is provided with a sample addition port 305 communicated with the outside atmosphere, a reaction chamber 301 and a waste liquid chamber 303 communicated with each other, and a first vent hole positioned on the top wall of the waste liquid chamber 303, so that a sample can normally flow, carry out immunoreaction and flow into the waste liquid chamber 303 after the immunoreaction is completed. A delay channel 302 for sample circulation is arranged between the reaction bin 301 and the waste liquid bin 303 to ensure the time required for the sample to carry out immunoreaction, so that the immunoreaction can be completed before the sample enters the waste liquid bin 303.

Meanwhile, a closed liquid storage bin 4 for containing cleaning liquid is further arranged on the chip body, one end of the liquid storage bin 4 is communicated with one end, close to the reaction bin 301, of the delay channel 302 through the injection channel 5, and an opening 401 is formed in the other end of the liquid storage bin. The chip body is also provided with a blocking piece which can seal the injection channel 5, and the opening 401 of the liquid storage bin 4 is connected with a pushing assembly which can seal the opening 401 and can slide along the length direction or the width direction of the liquid storage bin 4. When cleaning liquid is not required to be injected into the micro-channel 3, the injection channel 5 is sealed by the separation piece, and the cleaning liquid is sealed in the liquid storage bin 4; when the cleaning liquid needs to be injected, the blocking piece is removed or moved, the injection channel 5 is conducted, and the pushing assembly is pushed to push the cleaning liquid in the liquid storage bin 4 into the injection channel 5 and enable the cleaning liquid to flow into the micro-channel 3. With the arrangement, the liquid storage bin 4 is closed, cleaning liquid is injected into the micro-channel 3 by pushing and squeezing the cleaning liquid, and the cleaning liquid is injected into the micro-channel 3 in a sealed circulation mode, so that the cleaning liquid can be prevented from contacting with the outside atmosphere in the injection process, and the problem that air is brought into the micro-channel 3 to form bubbles when the cleaning liquid is injected is avoided; the pushing speed can be adjusted, and the injection speed of the cleaning liquid can be controlled so as to avoid the problem that the injection speed of the cleaning liquid is easy to be too fast or too slow in the prior art; the injection amount of the cleaning liquid into the micro flow channel 3 can also be adjusted.

As shown in fig. 2, in this embodiment, the single-channel push-and-flow microfluidic chip further includes a first vent hole 306 located on the top wall of the reaction chamber 301. And the bottom walls of the reaction bin 301 and the delay channel 302 are flush, the distance between the top wall and the bottom wall of the reaction bin 301 is greater than the distance between the top wall and the bottom wall of the delay channel 302, and the communication port between the top wall and the bottom wall is also smaller than the height of the reaction bin 301, so that the air pressure intensity in the delay channel 302 is higher than that in the reaction bin 301, and liquid and gas in the reaction bin 301 are not easy to enter the delay channel 302. The sample is first accumulated in the reaction chamber 301 and then slowly flows into the delay passage 302 through the communication port when the sample is injected. After the air carried by the injected sample enters the reaction chamber 301, the air is extruded to the top of the reaction chamber 301 or escapes through the first vent hole 306 as the injection amount of the sample increases, so that the problem that the detection of the conjugate of the immunoreaction is affected because air bubbles are formed in the micro flow channel 3 after entering the delay channel 302 is solved.

In a preferred embodiment of this embodiment, in order to ensure that air bubbles do not enter the delay channel 302, the cross-sectional area of the delay channel 302 is smaller than that of the reaction chamber 301. So configured, the pressure differential between the reaction chamber 301 and the delay channel 302 can be enhanced. Because the height of the reaction chamber 301 is higher than that of the delay channel 302, and the cross-sectional area is larger than that of the delay channel 302, the communication port between the delay channel 302 and the reaction chamber 301 is located at the bottom of the reaction chamber 301, the sample can flow into the delay channel 302 in a circulating manner, and the air bubbles floating above the sample can be retained in the reaction chamber 301. So set up, the single channel pushes away formula of irritating microfluidic control chip that this embodiment provided not only can solve the cleaning solution and can carry the air when annotating and form the problem of bubble in the microchannel, can also solve the problem that can form the bubble when the sample is annotated.

In particular, the pushing assembly may be an integral piston cylinder having a cross-section matching that of the reservoir 4. Alternatively, as shown in FIG. 1, the pushing assembly comprises a piston 402 having a cross-section matching the cross-section of the reservoir 4 and a push rod 403 insertable into the opening 401 of the reservoir 4. The length of the push rod 403 is longer than that of the liquid storage bin 4. Piston 402 and catch bar 403 are two parts, and the catch bar is located the liquid storage tank 4, can be better shutoff opening 401 of liquid storage tank 4, and piston 402 is located outside the liquid storage tank 4, just inserts in the opening 401 when needing to use and promotes. Compared with the integrated piston column, the pushing assembly with the structure can avoid the problem that the piston column is pushed when cleaning liquid is not required to be injected due to mistaken touch of the piston column, and is more convenient to package and transport.

Further, as shown in fig. 1 and 5, a sealing film 404, such as an aluminum foil or a plastic film, may be bonded to the opening 401 to seal the opening 401.

And the barrier may be a flap or a membrane, with a cross-section matching the cross-section of the injection channel 5, attached inside the injection channel 5. When the pushing rod 403 pushes the cleaning solution, the membrane may be broken or the connection between the blocking sheet and the injection channel 5 may be broken, so that the injection channel 5 is conducted. Alternatively, the barrier member is a film bag, such as a bag made of aluminum foil or plastic film, which is disposed in the reservoir 4 and wraps the cleaning solution. The film bag wraps the cleaning solution to form cleaning solution bubbles. When the pushing rod 403 pushes the bubbles of the cleaning solution, the membrane bag is punctured to allow the cleaning solution to flow out into the injection passage 5.

Further, on the basis that the barrier member is a film bag, as shown in fig. 1 and 2, the storage chamber 4 may be provided with a puncture hole 405 for the puncture needle 6 to extend into to puncture the film bag. With such an arrangement, the convenience of operation can be increased, and the requirement for rigidity of the push rod 403 can be reduced. In order to avoid air from entering the liquid storage chamber 4 from the puncture hole 405 to form air bubbles, one end of the puncture needle 6 far away from the needle point is provided with a sealing plug protruding out of the needle body. After the needle tip is inserted into the liquid storage bin 4, the blocking plug can block the orifice of the puncture hole 405.

The chip body of the single-channel push-filling type micro-fluidic chip can be formed by injection molding, and can also be composed of a substrate 2 and an upper cover 1 which is buckled and connected with the substrate 2. As shown in fig. 1 and 2, the substrate 2 has a cavity, and the upper cover 1 is used to close the cavity and form the closed micro flow channel 3. The liquid storage bin 4 can be positioned on the upper cover 1 and protrudes out of the lower surface of the upper cover 1, and a notch for clamping the liquid storage bin 4 is arranged at the position, corresponding to the liquid storage bin 4, on the substrate 2. After the upper cover 1 and the substrate 2 are buckled, the liquid storage bin 4 is clamped in the gap and is basically flush with the reaction bin 301.

As shown in fig. 2, 3 and 4, the reservoir 4 is provided with a communication port for allowing the cleaning liquid to flow out and communicating with the injection passage 5. The communication port is located on the lower surface of the upper cover 1 above the first end of the cavity in the substrate 2 forming the injection channel 5. After the upper cover 1 is fastened with the substrate 2, the communication port is aligned and overlapped with the first end of the cavity, so that a structure that the first end of the injection channel 5 is communicated with the communication port and the second end is communicated with one end of the delay channel 302 close to the reaction bin 301 is formed. And the puncture hole 405 can be located the lateral wall of stock solution storehouse 4, also can be located the roof of stock solution storehouse 4 and be located the upper surface of upper cover 1 promptly, then puncture hole 405 can be aligned with the intercommunication mouth.

In the prior art, the reagents that immunoreact with the sample are usually chemically solidified with the chip body to be encapsulated in the micro flow channel 3. When the sample is injected into the micro flow channel 3 and flows through the solidification region, the sample contacts with the reagent and an immunoreaction occurs. Such a method is likely to cause problems such as insufficient contact between the reagent and the sample, insufficient progress of the immune reaction, and a large number of samples required; and the reagent and the chip body are high in cost and complicated in flow, and the requirements on the structure of the micro-channel 3 and the material and transportation requirements of the chip body are extremely high, so that the production difficulty is high. In order to solve the above problem, in this embodiment, the micro flow channel 3 further includes a cartridge 304 communicating with the reaction chamber 301, and a solid reagent ball is disposed in the cartridge 304. The reagent ball comprises an antibody/antigen capable of immunoreacting with a sample, and the antibody/antigen is coated with a fluorescent marker and magnetic beads. As shown in fig. 1, the sample addition port 305 is located at a position corresponding to the sample addition chamber 304 in the upper cover 1, the sample addition port 305 communicates with the sample addition chamber 304, and the sample is injected into the sample addition chamber 304 from this point, so that the reagent ball is dissolved in the sample, and the reagent and the sample are completely mixed and sufficiently contacted. Thus, the reagent ball is arranged in the micro-channel 3, and the production flow that the liquid-shaped antibody/antigen coated with the fluorescent marker, the capture antibody/antigen and the chip body are solidified into a whole in the prior art is omitted; the reagent ball is solid, is easier to package, store and guarantee quality compared with liquid, and reduces the material requirement, the production requirement and the transportation and storage requirement of the single-channel push-irrigation type microfluidic chip. Compared with the prior art, the single-channel push-irrigation type microfluidic chip is arranged, so that the reagent can be completely dissolved in the sample, fully mixed with the sample and fully performed to ensure the immune reaction, and the reagent can flow together with the sample so as to perform the immune reaction in the whole flowing process. This embodiment provides a better way to ensure the chemical conditions for the immune response to occur sufficiently.

Then, the mixed sample flows from the sample addition chamber 304 into the reaction chamber 301 through the communication channel 308 between the sample addition chamber 304 and the reaction chamber 301, and is further mixed uniformly and immunoreaction occurs. The flow process of the sample and the separately arranged reaction bin 301 can ensure that the sample and the fluorescent marker are fully mixed and immune reaction can be rapidly and comprehensively carried out.

As shown in FIG. 1, a capturing chamber 307 is disposed between the reaction chamber 301 and the waste liquid chamber 303, and a magnet is disposed on the bottom wall of the capturing chamber 307. Since the magnetic beads are coated with the antibodies/antigens contained in the reagent beads, the magnetic beads are also coated with the conjugates generated by immunoreaction with the reagent beads, and the magnets can adsorb the conjugates. So configured, when the mixed sample flows through capture chamber 307, the binding substance produced by the immunoreaction will be captured and collected in capture chamber 307 and the remaining sample flows into waste chamber 303. The magnets may be laid over the entire area of the bottom wall of the capture chamber 307. The immunoreaction is fully carried out before the mixed sample flows into the capturing chamber 307, and then the mixed sample flows into the capturing chamber 307, so that the generated conjugate can be captured basically, and the capturing amount can be ensured.

It should be noted that the antibody/antigen coated with fluorescent label and magnetic bead is a product existing in the prior art.

The delay path 302 may be a helical meander, a V-meander, or other shaped meander. Preferably, as shown in fig. 3, the delay channel 302 is provided with a plurality of capillary flow channels, the plurality of capillary flow channels are arranged along the direction from the reaction chamber 301 to the capture chamber 307, and the plurality of capillary flow channels are sequentially communicated end to form the delay channel 302 in an S-shaped structure. The preferred dimensions of the capillary flow channel are 500 microns wide by 20 microns high, with width referring to the dimension perpendicular to the direction of reaction chamber 301 to capture chamber 307. By the arrangement, the sample can flow in a zigzag manner to slow down the time of the sample passing through the delay channel 302 so as to ensure the required time of the sample immunoreaction, and the sample can flow forwards through capillary characteristics, so that the fluidity of the sample is promoted, and the application of additional flow thrust can be avoided; and the arrangement direction of the plurality of capillary flow channels is consistent with the direction from the reaction chamber 301 to the capture chamber 307. So set up, can control the inclination of microchannel 3 through the inclination of adjustment chip body to can adjust the time that mixed sample flows through circuitous time delay passageway 302, make the time of immunoreaction can carry out actual adjustment according to the difference of sample. Not only can the duration of the immune reaction be ensured, but also the whole process from injection to detection completion of the slow sample due to excessive time waste can be avoided.

In order to ensure that the reagent dissolved in water is fully and uniformly mixed with the sample, a communication channel 308 is arranged between the sample adding bin 304 and the reaction bin 301; the cross-sectional area of the communication channel 308 gradually decreases from the sample loading chamber 304 to the reaction chamber 301. I.e., the communication channel 308 is a relatively narrow structure. The communication passage 308 may be a communication hole or a communication port, and the cross-sectional area of the entire passage is narrow. E.g. a slit structure. The smallest cross-sectional dimension of the communication channel 308 may be in the order of micrometers, with a preferred dimension of 200 micrometers. The gathering and circulating effects of the fluid liquid by the communicating channel 308 can achieve the effect of uniform mixing.

As shown in fig. 2-4, the reaction chamber 301 is formed by a first groove on the upper surface of the substrate 2 and a second groove on the lower surface of the upper cover 1, the second groove is surrounded by a protruding piece protruding from the lower surface of the upper cover 1, and the protruding piece is inserted into the first groove to form a snap fit. The sample loading chamber 304 is also formed by the engagement of a groove on the upper surface of the substrate 2 and a groove on the lower surface of the upper cover 1. It should be noted that, in fig. 1 to 4, each reference numeral indicates the position of each component constituting the single-channel push-and-flow microfluidic chip, and does not indicate the specific structure of the component. In FIG. 3, reference numeral 301 denotes a first groove for forming a reaction chamber and reference numeral 304 denotes a groove for forming a sample chamber, and in FIG. 4, reference numeral 301 denotes a second groove for forming a reaction chamber and reference numeral 304 denotes a groove for forming a sample chamber. After the top cover 1 and the substrate 2 are buckled and connected, the grooves on the top cover and the substrate are buckled and formed into a structure which forms a reaction chamber 301 and a sample adding chamber 304.

The delay channel 302 is communicated with the first groove of the reaction chamber 301, and a structure is formed in which the height of the reaction chamber 301 is higher than that of the delay channel 302 and the communication opening of the two is smaller. The two second grooves of the sample adding bin 304 and the reaction bin 301, which are both positioned on the upper cover 1, are communicated through a slit, the two first grooves of the substrate 2 are communicated through a slit, and after buckling, the sample adding bin 304 and the reaction bin 301 which are communicated through a slit are formed. The reagent ball can be inserted into the groove of the sample loading bin 304 during production, and then the upper cover 1 and the substrate 2 are buckled and connected. So that the reagent balls are integrally packaged in the chip body and integrally transported. In order to avoid the quality change of the reagent balls in the transportation process, the reagent balls can be frozen and then plugged into the sample loading bin 304.

It should be noted that the lower surface of the upper cover 1 and the upper surface of the substrate 2 refer to two surfaces that are in contact and engaged with each other, and the upper and lower refer to the upper and lower directions of the single-channel push-and-flow microfluidic chip when placed in the posture shown in fig. 1, that is, the upper and lower directions of fig. 1.

The first vent hole and the second vent hole 306 are both positioned on the upper cover 1, one end of the first vent hole is communicated with the atmosphere, and the other end of the first vent hole is communicated with the waste liquid bin 303. As shown in fig. 5, a sticker may be attached to the upper surface of the upper cover 1, and a model identifier, such as a character identifier or a two-dimensional code identifier, of the single-channel push-and-fill type microfluidic chip may be set on the sticker, so that an operator or a full-automatic detector may identify each single-channel push-and-fill type microfluidic chip for different detection contents. The position of the sticker corresponding to the capturing chamber 307 is set as a hollow area to avoid blocking the capturing chamber 307 and affecting detection.

It is understood that the same or similar parts in the above embodiments may be mutually referred to, and the same or similar parts in other embodiments may be referred to for the content which is not described in detail in some embodiments. The utility model provides a plurality of schemes contain the basic scheme of itself, mutual independence to restrict each other, but it also can combine each other under the condition of not conflicting, reaches a plurality of effects and realizes jointly.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A single-channel push-filling type microfluidic chip is characterized by comprising a chip body, wherein a closed microfluidic channel (3) for a sample to flow is arranged on the chip body, the microfluidic channel (3) comprises a sample adding port (305) for injecting the sample, a reaction bin (301) and a waste liquid bin (303) which are mutually communicated, and a first vent hole positioned on the top wall of the waste liquid bin (303), and a delay channel (302) for the sample to flow is arranged between the reaction bin (301) and the waste liquid bin (303);

the chip body is also internally provided with a closed liquid storage bin (4) for containing cleaning liquid, one end of the liquid storage bin (4) is communicated with one end, close to the reaction bin (301), of the delay channel (302) through an injection channel (5), the other end of the liquid storage bin is provided with an opening (401), and the single-channel push-irrigation type microfluidic chip further comprises a push assembly and a blocking piece, wherein the push assembly is slidably sealed at the opening (401) of the liquid storage bin (4), and the blocking piece is used for closing the injection channel (5).

2. The single-channel push-and-flow microfluidic chip of claim 1, wherein the pushing assembly comprises a piston (402) located in the reservoir (4) and adapted to the cross section of the reservoir (4), and a push rod (403) extending into the opening (401) and used for pushing the piston (402) to slide along the length direction of the reservoir (4), and the length of the push rod (403) is greater than that of the reservoir (4).

3. The single-channel push-and-flow microfluidic chip according to claim 2, wherein a sealing film (404) for sealing the opening (401) of the reservoir (4) is disposed at the opening.

4. The single-channel push-and-flow microfluidic chip of claim 2, wherein said barrier is provided as a film bag built into said reservoir (4) and used to wrap said cleaning fluid.

5. The single-channel push-and-flow microfluidic chip according to claim 4, further comprising a puncture needle (6), wherein a puncture hole (405) for the puncture needle (6) to extend into is formed in the reservoir (4), and a blocking plug for blocking the puncture hole (405) is arranged at one end of the puncture needle (6) far away from the needle tip.

6. The single-channel push-and-flow microfluidic chip of claim 1, further comprising a second vent hole (306) located on the top wall of the reaction chamber (301) and communicated with the reaction chamber (301), wherein the bottom walls of the reaction chamber (301) and the delay channel (302) are flush, the distance between the top wall of the reaction chamber (301) and the bottom wall of the reaction chamber is greater than the distance between the top wall of the delay channel (302) and the bottom wall of the delay channel, and the cross-sectional dimension of the delay channel (302) is smaller than the cross-sectional dimension of the reaction chamber (301).

7. The single-channel push-and-flow microfluidic chip according to claim 5, wherein the chip body comprises a substrate (2) provided with a cavity and an upper cover (1) connected to the substrate (2), the upper cover (1) closing the cavity and forming the microfluidic channel (3).

8. The single-channel push-filling type microfluidic chip according to claim 7, wherein the reservoir (4) is located on the upper cover (1) and protrudes from the lower surface of the upper cover (1), the reservoir (4) is provided with a communication port for the cleaning solution to flow out, the substrate (2) is provided with a notch for accommodating the reservoir (4) and the injection channel (5) for communicating the communication port with the delay channel (302), and the puncture hole (405) is aligned with the communication port.

9. The single-channel push-and-flow microfluidic chip according to claim 1, wherein the micro flow channel (3) further comprises a sample loading chamber (304) communicated with the reaction chamber (301), and a capture chamber (307) located between the delay channel (302) and the waste liquid chamber (303), the sample loading port (305) is located in the sample loading chamber (304), and a solid reagent ball immunoreactive with the sample is disposed in the sample loading chamber (304), the reagent ball comprises an antibody/antigen coated with a fluorescent label and a magnetic bead, and a magnet for adsorbing a conjugate generated by immunoreaction with the antibody/antigen is disposed on a bottom wall of the capture chamber (307).

10. The single-channel push-and-flow microfluidic chip of claim 9, wherein a communication channel (308) is disposed between the sample loading chamber (304) and the reaction chamber (301); the cross-sectional area of the communication channel (308) is gradually reduced along the direction from the sample loading bin (304) to the reaction bin (301); the delay channel (302) is provided with a plurality of capillary flow channels, the capillary flow channels are arranged along the direction from the reaction bin (301) to the capture bin (307), and the capillary flow channels are sequentially communicated end to form the delay channel in an S-shaped structure.