JP3193194U - Syringe and serum transfer kit - Google Patents

Abstract

Provided are a syringe that minimizes the number of operations to be performed when acquiring serum in biochemical analysis and then transferring the serum to an analyzer, and a serum supply kit including such a syringe.
A) collecting body fluid in a storage element reservoir; b) centrifuging the storage element storage fluid; and subsequently c) sending a body fluid sample that pushes the body fluid from the storage element reservoir 24 to an analysis system. A syringe comprising a horizontally oriented blood barrel whose first end is an outlet and whose second end is sealed by a plunger, wherein the lateral side of the barrel includes a hole, the hole and the barrel The distance of the second end is smaller than the length of the plunger, and the hole is completely covered by the plunger when the plunger is pushed into the barrel.
[Selection] Figure 5

Description

The present invention relates to a method for supplying a body fluid sample, in particular blood, to an analysis system.
The present invention also relates to a syringe for sucking blood and supplying serum to a biomedical test, and a serum supply kit including such a syringe.

Today, various blood tests are usually medical tests.
A blood sample collected from a patient is subjected to separation of blood cells (hematocytes, hematocrit) from the plasma (serum) and finally an appropriate biochemical test on the serum sample.

A blood sample from the patient is taken using a standard syringe using a needle or a currently special vacuum tube. The tube is made of glass or plastic and has a tight stopper and an element adapted to the needle. This needle is inserted into the blood vessel, and initially the empty tube is slightly filled with blood. Thereafter, the tube is disconnected from the needle, resulting in a blood-containing tube sealed with a stopper.
If necessary, another empty tube may subsequently be connected to the needle.
Usually, both elements described above can be discarded due to the need for sterilization. Such tubes with needles are described, for example, in US2010323437 A1, KR 20080025050 A1, or JP 2000189406 A. Typically, these tubes have a length of a few centimeters and a volume of a few hundred microliters to a few milliliters.
Alternatively, a drop of capillary blood can be collected from a finger and collected in a capillary wetted with blood. In such a method, blood is collected in a capillary, appropriately prepared, filled in a tube connected to the capillary, and the capillary is removed after blood collection is completed.

Blood cells are separated from the plasma in most cases by centrifuging the blood sealed in the container. For example, the tube is in a centrifuge.
This centrifugation causes blood cells (hematocrit) to settle to the bottom of the tube and plasma (serum) accompanies them.

  The tubes thus prepared are placed in a rack almost vertically and analyzed by biochemical analysis.

  Biochemical analysis is performed by an automated instrument to test the chemical composition of serum. For example, the method is used to determine the blood concentration of the following substances: glucose, lipids (eg cholesterol, triglycerides), yeast or ions. With a set of needles and a suitable mechanism, the analyzer draws a serum sample from the tube and mixes it with a suitable selected reagent in a small cuvette, for example, analyzing the product of the chemical reaction by photometric analysis, and then analyzing the target Determine the concentration of the substance.

  Commercially available equipment is used to perform the biochemical analysis based on the above method and the test method described above (eg ELITech Flexor XL systems). These have become the subject of many patent applications and patents (eg JP8211072A, JP101327354A, US20040185549A1, US20050014274A1, US4808380, US61612399, or US20070065945A1).

Another group of analyzers are systems that use disposable discs. For example, the commercial models Abaxis Piccolo Xpress and Samsung IVD-A10A, which contain the chemical reagents necessary to perform a diagnostic reaction (lyophilized reagent) and allow the use of the necessary biochemical materials. In these designs, biochemical material is obtained in advance from the patient and transferred using an external infusion device (pipette). This method element not only increases the number of operations required to perform the analysis, but also extends the time required to perform the test. The number of analyzes performed using a disk-based analyzer is limited by the availability of reagents. That is, it is limited by the reagents necessary to perform accurately after the lyophilization process. In such a system, the reagent is dispensed using centrifugal force to compensate for the uniform dispersion of the biochemical material and solvent, and the lyophilized reagent is stored and located where the chemical reaction takes place.
The limitations that are essentially necessary for the accuracy of measurements made by analyzers based on disk technology are related to the ability to reproduce and dispense small fractions of lyophilized reagents.

US Patent Application Publication No. 2010/0323437 Korean Published Patent No. 2008-0025050 JP 2000-189406 A Japanese Patent Application Laid-Open No. 08-211072 JP-A-10-132754 US Patent Application Publication No. 2004/0185549 US Pat. No. 4,808,380 US Pat. No. 6,161,399 US Patent Application Publication No. 2007/0065945

  From the above description, the process of obtaining the serum required for medical testing and transfer of serum samples to the analyzer is complex, eroding the patient's anatomy, requiring a lot of work, especially filling the tube. It is necessary to centrifuge the tube and aspirate the serum multiple times from the tube for subsequent analysis. In particular, methods known today often require that samples and serum be transferred between different containers or hydraulic tubes, and that a hydraulic connection between these containers or tubes be made manually or mechanically. Such a reduction in the number of operations performed on samples and serum is highly desirable. This is because it simplifies the procedure and reduces errors and contamination as much as possible. Simplifying the transfer of blood plasma (serum) to the analyzer is preferable because it shortens the time from drawing blood from the patient to performing the actual analysis.

  The object of the present invention is to minimize the number of operations performed in obtaining serum in biochemical analysis and then transferring the serum to the analyzer.

According to the present invention,
a bodily fluid sample characterized by: a) collecting body fluid in the storage element reservoir; b) centrifuging the body fluid in the storage element reservoir; and c) extruding body fluid from the storage element reservoir (4, 24). It is to provide a method for sending to an analysis system.

  In the present invention, the body fluid is preferably blood.

In the above step b), it is preferable to separate plasma and blood cells by centrifugation.

  In the above step b), it is preferable to separate into serum and hematocrit by centrifugation.

In step c) above, plasma or serum is preferably extruded into the analysis system.

  In the present invention, a microfluidic system is preferably used as the storage element.

  In the present invention, in step c), the body fluid is preferably pushed out of the microfluidic system chamber by pumping the liquid into the microfluidic system to the analysis system.

  In the present invention, it is preferable to use a liquid that is immiscible with the body fluid.

  In another preferred embodiment of the present invention, a syringe is preferably used as the storage element.

  In this case, in step c) it is preferred that the body fluid is pushed out of the storage element reservoir into the analysis system by movement of the plunger into the microfluidic system, preferably movement by the operator's palm or a suitable mechanism.

  In this case, in step c) above, the body fluid is preferably pushed out of the syringe reservoir into the analysis system by pumping the liquid with the syringe.

  The present invention is also a syringe comprising a blood barrel surrounded by a lateral side, having a first end as an outlet and a second end sealed by a plunger, wherein the lateral side of the barrel includes a hole, the hole and the barrel The second end distance is less than the length of the plunger and relates to a syringe that is completely covered by the plunger by pushing the plunger into the barrel.

  In the present invention, it is preferable to form a capillary tube having an inner side surface of the barrel having an inner diameter of 0.2 to 4 mm, preferably 1 to 2 mm.

  In the present invention, the inner surface, hole or fleur of the barrel is coated with a blood clotting promoting substance selected from the group consisting of silica, colloidal silica, kaolin, glass particles, diatomas earth, thrombin-based material, and elastic acid. Is preferred.

  Also, instead of this, the inner surface of the barrel, hole or fleur is EDTA (ethylenediaminetetraacetate), trisodium citrate, heparinates (sodium, lithium, ammonia salt of heparin), potassium oxalate, sodium fluoride, It is preferably coated with a substance that prevents blood coagulation, selected from the group consisting of hirudin, acetic acid iodide or thrombin inhibitors (eg, PPACK or agratroban).

  In the present invention, it is preferable that the capillary end does not wet the serum.

  The syringe is a syringe as described above, and includes a microfluidic nozzle that can be fitted to an outlet in the barrel of the syringe, and a centrifuge rotor having a well for positioning the syringe together with the microfluidic nozzle to be attached to form a kit, Wells also relate to serum delivery kits that can be adapted to the size and form of the syringe along with the nozzle.

  In the present invention, it is preferable that the centrifugal rotor has a hole apart from the centrifugal axis of rotation, and the plunger is pushed through the hole, while the syringe is disposed in the well of the rotor.

  In the present invention, it is preferable that the centrifuge rotor has a hole at the position where the outlet of the syringe is located, so that serum can be transferred from the syringe to the analyzer.

  Preferred specific examples of the syringe according to the present invention are as follows.

  According to the present invention, the syringe is composed of a blood barrel that is surrounded by a lateral side, the first end is an outlet, and the second end is sealed by a plunger, the lateral side of the barrel including a hole, The distance between the second end of the barrel is smaller than the length of the plunger, and the syringe is configured to completely cover the hole by the plunger when the plunger is pushed into the barrel.

  In the present invention, the length of the plunger is preferably such that after the plunger is completely pushed into the barrel, the end of the plunger faces the end of the syringe.

  The outlet is preferably compatible with the capillary end.

  The inner surface of the barrel forms a capillary tube and has an inner diameter of 0.2 to 4 mm, preferably 1 to 2 mm.

  The length of the barrel is 10 to 200 mm, more preferably 30 to 70 mm.

  The hole is circular and has a diameter of 0.2 mm to 4 mm, more preferably 1 to 2 mm.

  The hole preferably has a funnel-shaped fleur to facilitate blood suction.

  The capacity of the syringe barrel is 20 to 100 millicubic meters, more preferably 25 to 250 millicubic meters, and most preferably 50 to 150 millicubic meters.

  In a preferred embodiment of the invention, the inner surface, hole or fleur of the barrel is coated with a substance that promotes blood clotting. This substance is selected from the group consisting of silica, colloidal silica, kaolin, glass particles, diatomas earth, thrombin-based material, and elastic acid.

  In another preferred embodiment of the present invention, the inner surface, hole or fleur of the barrel is made of EDTA (ethylenediaminetetraacetate), trisodium citrate, heparinates (sodium, lithium, ammonia salt of heparin), potassium oxalate, fluorine It is preferably coated with a substance that prevents blood coagulation selected from the group consisting of sodium iodide, hirudin, acetic acid iodide or thrombin inhibitors (eg PPACK or agratroban).

  The capillary end is preferably not wetted by serum.

  The syringe preferably has a holder located along and connected to the side wall of the barrel.

  The syringe preferably has a finger support tab in the vicinity of the second end of the barrel.

  The present invention is also a serum delivery kit, the above-mentioned syringe, a microfluidic nozzle that can be fitted to the outlet of the syringe barrel, and a centrifuge rotor having a well for positioning the syringe together with the microfluidic nozzle to be attached; The well is a kit that is adaptable to the size and configuration of the syringe along with the nozzle.

  It is preferable to have a hole of the centrifuge rotor at the end away from the rotation axis of the centrifuge, and to push the plunger of the syringe through the hole, while positioning the syringe in the well of the rotor.

  The centrifuge rotor preferably has a hole in which the exit of the syringe is located, and enables transfer of serum from the syringe to the analyzer.

  In the present invention, a “microfluidic system” refers to a system including a channel including at least one characteristic dimension (eg, diameter) not exceeding 2 mm. The microfluidic system is preferably made from a polymer, such as polycarbonate, polypropylene, polystyrene, or other polymer suitable for injection molding. Here, a lamellar system is particularly preferred, in particular the upper layer having holes and the lower layer having microfluidic channels.

The present invention has the following advantages.
・ The storage tank and tube for collecting and transferring the collected body fluid are connected with hydraulic pressure, separated into components using centrifugal force, and the sample (plasma / serum) is directly used in an easy way. Minimize the manual and mechanical operations required to transfer;
Minimize physical methods of bodily fluids that must be moved to use serum (plasma) from collection. This reduces the volume of bodily fluid lost and increases safety in the case of dangerous samples (eg pathogens). Minimize the surface area of containers and tubes that come into contact with body fluids if necessary;
Reduce the volume of a single specification material that is subject to distribution in the collection, separation and use of body fluids. -Facilitates handling of small volume samples of body fluids. And simplify the equipment needed to automate these tasks and minimize possible user errors;
Minimize the time between transfers of plasma (serum) separated from the blood aspiration required for analysis to the analyzer.

It is a schematic diagram of the empty syringe in the preferable example of this invention connected to the nozzle. FIG. 2 is a schematic view showing a state where blood is filled in the syringe of FIG. 1 connected with a nozzle. FIG. 2 is a schematic diagram showing a state in which the syringe of FIG. 1 is filled with blood and positioned in the centrifugal rotor of the present invention. It is a schematic diagram which shows the state which transfers serum to the analyzer through the nozzle from the syringe of this invention. 1 is a schematic diagram of a microfluidic system (chip) that is a specific example of the present invention. FIG. FIG. 6 is a schematic diagram of the microfluidic system of FIG. 5 filled with a blood sample. FIG. 7 is a schematic diagram showing a state where the microfluidic system of FIG. 6 is positioned in the centrifugal rotor of the present invention. FIG. 7 is a schematic diagram illustrating the microfluidic system of FIG. 6 after completing centrifugation and separating blood components from serum.

The present invention will be described in detail with reference to the accompanying drawings.
Example 1-Transferring Sample Using Syringe-Syringe 1
Assemble the syringe. Here, the blood barrel 1 is made of a polystyrene tube (outer diameter 3 mm, inner diameter 2 mm, length 70 mm), and has a hole with a diameter of 2 mm on the side wall of the tube. On the other hand, the center of the hole 2 is located 4 mm from the end of the tube. This tube end is used for the insertion of the plunger 3. Preferably, the hole 2 has a funnel-shaped fleur to facilitate the suction of blood into the syringe. This syringe is compatible with the plunger 3 and has the following dimensions. Outer diameter (position to be sealed) -2.05mm, outer diameter (on remaining length) -1.98mm, length -8mm. Plunger 3 is manufactured from Dyneon (Plunger 3 is made from other polymers such as, but not limited to, polyvinyl chloride (PVC), polypropylene (PP), Teflon, Dyneon, natural, isoprene, or nitrile rubber Or a hard core-soft shell composite-sealing; most typical polymers can be used). The syringe volume here is 200 μL. Based on the above description, a person skilled in the art can make a syringe based on the present invention, and the desired capacity ranges from 20 μL to 1000 μL by adjusting the length and / or diameter of the syringe barrel 1. Plunger 3 is set in a tube with a depth of 3 mm so that hole 2 is not covered.

  The optional part of the syringe is a holder (not shown) —the holder (eg, rectangular form) may be placed along the tube of the syringe body or connected to the body. It can also be created in the form of a flat fin that is attached to the syringe body. Such holders can also be used for labeling collected samples (eg barcode labels). Tube 1, hole 2 and fleur (not shown) contain substances on the inner surface that are distributed by evaporation. Such substances may be preferred with respect to the final result of blood separation. These substances may be used to prevent blood clotting, for example, but not limited to, EDTA (ethylenediaminetetraacetic acid salt), trisodium citrate, heparinates (ammonium salts of sodium, lithium, heparin) ), Hirudin, potassium oxalate, sodium fluoride, acetic acid iodide, or thrombin inhibitors (eg, PPACK or agratroban). These substances may be used to activate blood clotting, for example, but not limited to, from the group consisting of silica, colloidal silica, kaolin, glass particles, diatomas earths, thrombin materials, and elastic acid. To be elected.

  The syringe according to the present invention can adopt a form in which the plunger 3 has a finger support.

  The syringe according to the present invention can adopt a form in which an inlet is further provided in the barrel 1 where the blood is located. It is preferable that serum (plasma) 15 is located after centrifugation and separation. Such an inlet is not shown in the drawing. This inlet can be centrifuged and separated to transfer liquid that is immiscible with serum (plasma) 15 (eg oil) and the desired fraction of serum (plasma) 15 can be pushed out of barrel 1 by the application of this immiscible fluid stream.

  The syringe according to the present invention is used as follows.

When the patient places his finger in hole 2, blood 10 is simultaneously sucked into the syringe by capillary force. In the case of the syringe described here, adults can fill it in 30 seconds while it is collected and only 0.2 ml is sufficient for blood tests, either by traditional syringe or blood collection tube as described in the introduction About 10 times less than the case. After filling the syringe with blood, the plunger 3 is pushed to a depth of 8 mm to completely fit the tube, and the plunger 3 closes the hole in the side wall. This plunger is designed to push into the end of the barrel 1 and not go too deep. At this end, the syringe has a capillary end 4, the syringe outlet is connected to the microfluidic nozzle 5, adapted to the serum sensor 7 and ends at the outlet 8. The nozzle 5 has a socket 9 and is preferably inserted into the terminal 4.

The syringe is positioned in the centrifuge rotor, the outlet 8 of the microfluidic nozzle 5 is directed in the direction of the rotation axis 11, and the plunger 3 protrudes from the rotation axis 11 so that the collected material 10 does not flow out during centrifugation. The centrifuge rotor has a well 12 that matches the size and configuration of the syringe. The rotor may have a fiberglass filler in aluminum (or other preferred material)-eg ABS, polyacetal (POM), polystyrene or polyamide 66. After centrifugation, blood 10 is separated into serum 15 and hematocrit 17, while serum 15 is located near rotary shaft 11 and at the syringe outlet, and hematocrit 17 moves away from rotary shaft 11 and the syringe outlet. Further, at the end, the rotor has a hole 13, so that the rod 14 is inserted from the outside and the plunger 3 is pushed. This is for starting the flow of the separated serum 15 and further transferring it to an analysis system (not shown). Serum 15 flows out of other holes in the rotor (near its center). In particular, the syringe can be connected to a nozzle that produces droplets 16, and such a combination is located in a centrifuge rotor. After blood collection and centrifugation, the necessary serum fraction is analyzed via nozzle 4 through the analysis system (Fig. This is because the syringe can be dispensed without being removed from the rotor. Preferably, the rotor may further have a hole at a position where the end of the syringe is located (position closer to the rotation shaft 11).

  Centrifugation must be performed by setting an appropriate time and rotation speed. Centrifugation parameters—time and rotational speed, eg 15 minutes 3000 rpm, 2 minutes 8000 rpm—these are well known to those skilled in diagnostic tests.

  After centrifugation, serum 15 is obtained in the syringe (closer to the syringe outlet), and blood cells 17 are obtained closer to the plunger 3. Therefore, when the plunger 3 is pushed into the tube 1, the serum 15 is pushed out from the syringe and can be transferred to the analysis system (not shown) through the microfluidic nozzle 4.

Example 2-Sample delivery using a syringe with a conventional plunger A conventional syringe with a plunger is centrifuged after the whole blood (possibly with the addition of anticoagulant anticagulant or coagulant) is filled into the plunger. Placed in the machine rotor, orienting the syringe outlet in the direction of the axis of rotation and the plan jar facing away from the axis of rotation to prevent the collected material from flowing out during centrifugation.

Centrifugation is performed with the following parameters.
After centrifugation, serum or plasma (located closer to the syringe outlet) and hematocrit (located closer to the plunger) are obtained in the syringe, so by pressing the plunger, the serum or plasma is Extruded from a syringe and transferred, for example, through a microfluidic nozzle to an analysis system.

A series of tests was performed for the system at centrifugation speeds at various rotational speeds. The table below displays the data from the analysis performed.
Rotational speed [rpm] Time [min] Serum volume [%] Plasma volume [%]
8000 2 32 54
8000 2 27 50
8000 2.5 46 54
8000 5 50 55
Since the plasma yield was higher than the serum yield, the further stage of the test focused mainly on obtaining the plasma.

The centrifugation test with anticoagulant (heparin) is displayed below.
Rotational speed [rpm] Time [min] Obtained plasma volume [%]
8000 2 41.28
8000 2 36.7
8000 3 42.1
8000 3 48.8
8000 5 52.8
8000 5 56.2
10000 2 45.1
10000 2 48.5
10000 3 51.9
10000 3 47.8
10000 5 55.9
10000 5 52.0
12000 2 50.9
12000 2 53.5
12000 3 49.3
12000 3 54.0
12000 5 53.2
12000 5 57.7
In the analysis, the plasma yield obtained in centrifugation (41% -58%) is consistent with the generally accepted criteria for centrifugation of whole blood (40% -60%).

Example 3 Sample Supply Using Microfluidic System (Chip) A two-layer microfluidic chip was assembled. There, the lower layer consists of a channel and a blood sample chamber. The top plate has three holes, including hole 23, which are applied to the blood sample directly from the patient's finger. Optionally, the hall 23 is configured so that a capillary or funnel can be placed for simple blood collection.
The hole 22 used to supply the serum (or plasma) to the analyzer is optional but suitable for the construction of a capillary for easy movement of the fluid.
For those skilled in the art, it is clear that each hole (21, 22, or 23) is used for blood sample collection, and furthermore, each hole can be placed on both the upper and lower plates, It can also be placed on the outside resulting from connecting the plates.
After centrifugation is complete, it is essential to use hole 21 with channel 25 to push the resulting serum (or plasma), and use hole 22 with channel 26 to transfer the serum to the analyzer To do. During this operation, the hall 23 must be closed.
The entrance of channel 25 to channel 24 allows for blood cells to be present at the entrance after separation, taking into account the biochemical diversity material collected. Both layers of the system are durability bonded so as to maintain the required durability throughout the microfluidic system.

  The total volume of the microfluidic system is about 150 μL in the specific example, and the inner width of the chip (25, 26 and 27) is 1 mm.

  The inner channel of the chip is preferably formed on the surface with substances formed by the desired deposition for the end result of blood separation, these substances are used to prevent blood from clotting, specifically Is not limited to EDTA (ethylenediaminetetraacetate), trisodium citrate, heparinates (sodium, lithium, ammonia salt of heparin), potassium oxalate, sodium fluoride, hirudin, acetic acid iodide, Or a thrombin inhibitor (eg, PPACK or agratroban). These materials can be used to activate blood clotting, and specifically include, but are not limited to, silica, kaolin, glass particles, diatomaceous earth, thrombin agents or ellagic acid.

The microfluidic system according to the present invention is used as follows.
The patient places a stabbed finger in the hole above the chip surface. Blood is spontaneously drawn into the system due to capillary forces. For the system discussed here, an adult fills it within approximately 30 seconds, and the amount of blood collected in this way is sufficient for a blood test is only 0.15 cubic centimeters, with a conventional syringe or blood collection tube. Therefore, it is about 10 times less. After filling the chip with blood, it is placed in a special rotor (Figure 3), and the centrifuge rotor has wells adapted to the size and shape of the system. The rotor is made of aluminum (or other suitable material such as ABS, polyacetal (POM), polystyrene or polyamide 66 with glass fiber filler)). After centrifugation, the blood is divided into plasma (or serum) and hematocrit, the plasma is near the axis of rotation of the microfluidic, the hematocrit is away from the axis of rotation, the rotor and tip have holes, and through the hydraulic system The oil is then delivered, and the oil is used to partially distribute the plasma to the system being analyzed (not shown). By pumping oil into the microfluidic system, some plasma is forced out of the system and the plasma flows out through the holes in the rotor.

  Centrifugation must be performed by setting the appropriate time and rotational speed. Centrifugation parameters, time and rotational speed (eg, 3 minutes at 8000 rpm) are well known to those skilled in diagnostic testing.

A series of tests with different rotation speeds and centrifugation times were performed, the table below shows the plasma yields obtained:
Centrifugation time [min] Rotational speed [rpm] Plasma yield [%]
2 3000 39
1 5000 31
3 8000 53
1.5 6000 48
2 5000 48
1 8000 52
3 6000 45
2.5 8000 50
2 8000 49
1 6000 43
3 3000 38
1.5 5000 43
2.5 3000 39
30 8000 43
2 6000 50
3 5000 50
1.5 8000 48
2.5 6000 52

For those skilled in the art, a specific plasma yield (adult: average 35 to 60%) can be obtained using other than the above rotation speed, centrifugation time, especially with higher rotation speed and shorter time. it is obvious.

1-barrel, 2-hole, 3-plunger, 4-capillary end, 5-microfluidic nozzle, 6-tube to send serum to the junction, 7-sensor, 8-outlet port, 9-microfluidic nozzle socket, 10-blood, 11-axis of rotation, 12-well, 13-hole, 14-rod, 15-serum, 16-droplet, 17-hematocrit, 21-transport fluid that pushes serum (or plasma) out of the barrel Vent holes used in the test, 22-vent holes used to transfer serum (or plasma) to the analyzer, 23-holes for blood aspiration, 24-barrel, 25, 26, 27-holes and barrels And the channel to connect

Claims (19)

  a bodily fluid sample characterized by: a) collecting body fluid in the storage element reservoir; b) centrifuging the body fluid in the storage element reservoir; and c) extruding body fluid from the storage element reservoir (4, 24). How to send to the analysis system.   2. The method according to claim 1, wherein the body fluid is blood. 2. The method according to claim 1, wherein in step b), plasma and blood cells are separated by centrifugation.
  2. The method according to claim 1, wherein in step b), serum and hematocrit are separated by centrifugation. The method of claim 1 wherein in step c) plasma or serum is extruded into the analytical system.
  A method according to any preceding claim, wherein a microfluidic system is used as the storage element. The method according to claim 6, wherein in step c) the body fluid is pumped from the microfluidic system chamber (24) by pumping the liquid into the microfluidic system.
  8. The method according to claim 7, wherein a liquid that is immiscible with the body fluid is used.   6. The method according to claim 1, wherein a syringe is used as the storage element.   10. In step c), the body fluid pushes the liquid from the microfluidic system chamber (24) to the analysis system into the microfluidic system by movement of the plunger, preferably by movement of the operator's palm or a suitable mechanism. Method.   11. The method according to claim 9 or 10, wherein in step c) the body fluid is pushed from the syringe reservoir to the analysis system by pumping of the liquid with a syringe.   A syringe comprising a horizontally-oriented blood barrel whose first end is an outlet and whose second end is sealed by a plunger, wherein the lateral side of the barrel (1) includes a hole (2), the hole (2) and the barrel The distance of the 2nd end of (1) is smaller than the length of a plunger, The syringe is characterized by completely covering a hole with a plunger by pushing the plunger (3) into the barrel.   13. Syringe according to claim 12, wherein the barrel (1) forms a capillary tube with an internal lateral side having an inner diameter of 0.2 to 4 mm, preferably 1 to 2 mm.   The inner surface, hole (2) or fleur of the barrel (1) is coated with a substance that accelerates blood clotting, selected from the group consisting of silica, colloidal silica, kaolin, glass particles, diatomous earth, thrombin-based material, and elastic acid. The syringe according to claim 12 or 13, which is used.   Inner surface of barrel (1), hole (2) or fleur is EDTA (ethylenediaminetetraacetate), trisodium citrate, heparinates (sodium, lithium, ammonia salt of heparin), potassium oxalate, sodium fluoride, hirudin 14. The method according to claim 12 or 13, which is coated with a substance for preventing blood coagulation selected from the group consisting of (hirudin), acetic acid iodide, or a thrombin inhibitor (for example, PPACK or agratroban). Syringe.   15. Syringe according to claim 12, 13 or 14, characterized in that the capillary end (4) does not wet the serum.   To position the syringe together with the syringe according to any of claims 12 to 16, a microfluidic nozzle (5) adaptable to the outlet to the barrel (5) of the syringe, and a microfluidic nozzle (5) attached Serum delivery kit, characterized in that the well can be adapted to the size and configuration of the syringe together with the nozzle.   The end of the centrifugal separation shaft (11) has a centrifuge rotor hole (13) through which the syringe plunger (3) is pushed, while the syringe is placed in the rotor well. The kit according to claim 17. 19. The kit according to claim 17 or 18, wherein the centrifuge rotor has a hole in which an outlet of the syringe is located, and serum can be transferred from the syringe to the analyzer.

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