CN216584943U - PCR plate and fluorescence PCR detector - Google Patents

Abstract

The utility model relates to a PCR plate and a fluorescent PCR detector. The PCR plate comprises a plate body, wherein the plate body is provided with a bottom surface and a top surface deviating from the bottom surface, and the top surface is inwards sunken to form a plurality of sample holes for containing sample liquid. In practical use, a sample solution is formed through nucleic acid extraction and purification, and the sample solution is transferred into the sample hole and placed in a storage environment for storage. When needs carry out the amplification and detect, arrange the PCR board in on the PCR detector, utilize the temperature control spare on the PCR detector and the bottom surface and the top surface heat conduction contact of PCR board, the bottom surface and the top surface that the sample liquid in each sample hole passes through the board body carry out the heat exchange with the temperature control spare, thereby control (intensification or cooling) the temperature of the sample liquid in each sample hole through the temperature control spare, heat conduction area is great, can be fast with the temperature control of the sample liquid in the sample hole in required temperature range, be favorable to improving the temperature raising and lowering efficiency, and then improve detection efficiency.

Description

PCR plate and fluorescence PCR detector

Technical Field

The utility model relates to the technical field of biological detection, in particular to a PCR plate and a fluorescent PCR detector.

Background

The real-time fluorescence quantitative PCR (polymerase chain reaction) technology is widely applied to a plurality of fields such as genetic disease molecular diagnosis, clinical examination, animal and plant import and export quarantine, food safety monitoring, soil microorganism detection, paternity test and the like. Samples such as blood, food, soil and the like contain a large amount of inhibitory factors, such as hemoglobin, methemoglobin, lactoferrin, humic acid and the like. Therefore, nucleic acids must be isolated from these test samples and then used for PCR amplification.

And storing the sample liquid obtained after nucleic acid extraction and purification by using a PCR tube, placing the PCR tube on a PCR detection instrument when amplification detection is required, and operating the PCR detection instrument to perform amplification detection on the sample liquid in the PCR tube. Conventionally, a PCR tube comprises a tube frame and a plurality of hollow tube columns fixedly connected to the tube frame for containing sample liquid, such as a PCR reaction connecting tube described in patent CN 210458197U. Need cool down or heat it when sample liquid is deposited and is enlargeed and detect, nevertheless because the special construction of PCR pipe, lead to the heat conduction area of PCR pipe less, go up and down the temperature inefficiency, influence detection efficiency.

SUMMERY OF THE UTILITY MODEL

Therefore, it is necessary to provide a PCR plate and a fluorescence PCR detector for overcoming the above-mentioned defects, aiming at the problems of the prior art, such as small heat conduction area of the PCR tube, low temperature increasing and decreasing efficiency, and low detection efficiency.

A PCR plate comprises a plate body, wherein the plate body is provided with a bottom surface and a top surface deviating from the bottom surface, and the top surface is inwards sunken to form a plurality of sample holes for containing sample liquid.

In one embodiment, the PCR plate further comprises a plurality of annular protrusions protruding from the top surface, the plurality of annular protrusions correspond to the plurality of sample wells one-to-one, and each annular protrusion is disposed around a corresponding one of the sample wells.

In one embodiment, the plate body is integrally formed with the plurality of annular protrusions.

In one embodiment, the top surface and the bottom surface are both adapted to be in conductive thermal contact with a temperature control member.

In one embodiment, the plurality of sample wells are arranged in an array.

In one embodiment, the number of the sample holes is 8, and the 8 sample holes are arranged at intervals along the longitudinal direction of the plate body.

In one embodiment, the number of the sample holes is 24, and the 24 sample holes are arranged in 3 rows and 8 columns.

In one embodiment, the number of the sample holes is 48, and the 48 sample holes are arranged in 6 rows and 8 columns.

In one embodiment, the number of the sample holes is 96, and the 96 sample holes are arranged in 12 rows and 8 columns.

In one embodiment, the PCR plate further comprises a cover detachably covering the top surface of the plate body.

A fluorescence PCR detector comprises a shell, a fluorescence detection mechanism and a PCR plate in any embodiment;

the fluorescence detection mechanism and the PCR plate are both arranged on the shell, and the fluorescence detection mechanism is used for carrying out fluorescence PCR detection on the sample liquid in each sample hole on the PCR plate.

In one embodiment, the PCR plate is mounted to the housing with the top surface of the PCR plate outside the housing and the bottom surface of the PCR plate within the housing;

the fluorescent PCR detector further comprises a cover plate and a first temperature control module, wherein the cover plate is constructed to cover the top surface of the PCR plate, and the first temperature control module is arranged on the cover plate.

In one embodiment, the fluorescent PCR detector further includes a second temperature control module disposed in the housing, and the second temperature control module is attached to the bottom surface of the PCR plate;

the second temperature control module is provided with a plurality of exposure holes which are formed in one-to-one correspondence with the plurality of sample holes, and the fluorescence detection mechanism carries out fluorescence PCR detection on the samples in the sample holes through each exposure hole.

In practical use, the PCR plate and the fluorescent PCR detector form a sample solution through nucleic acid extraction and purification, and the sample solution is transferred into the sample hole and placed in a storage environment for storage. When needs carry out the amplification and detect, arrange the PCR board in on the PCR detector, utilize the temperature control spare on the PCR detector and the bottom surface and the top surface heat conduction contact of PCR board, the bottom surface and the top surface that the sample liquid in each sample hole passes through the board body carry out the heat exchange with the temperature control spare, thereby control (intensification or cooling) the temperature of the sample liquid in each sample hole through the temperature control spare, heat conduction area is great, can be fast with the temperature control of the sample liquid in the sample hole in required temperature range, be favorable to improving the temperature raising and lowering efficiency, and then improve detection efficiency.

Drawings

FIG. 1 is a schematic structural diagram of a PCR plate according to an embodiment of the present invention;

FIG. 2 is a front view of the PCR plate shown in FIG. 1;

FIG. 3 is a sectional view of the PCR plate shown in FIG. 2 taken along the A-A direction;

FIG. 4 is a side view of the PCR plate shown in FIG. 2;

FIG. 5 is a schematic view of a PCR plate according to another embodiment of the present invention;

FIG. 6 is a schematic structural view of a PCR plate according to another embodiment of the present invention;

FIG. 7 is a schematic structural view of a PCR plate according to another embodiment of the present invention;

FIG. 8 is a schematic structural view of a fluorescent PCR detector according to an embodiment of the present invention (closed state);

FIG. 9 is a schematic view of the fluorescent PCR detector shown in FIG. 8 (uncapped state);

FIG. 10 is a top view of the fluorescent PCR detector shown in FIG. 9.

Detailed Description

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in detail below. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein.

In the description of the present invention, it is to be understood that the terms "central," "longitudinal," "lateral," "length," "width," "thickness," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," "clockwise," "counterclockwise," "axial," "radial," "circumferential," and the like are used in the orientations and positional relationships indicated in the drawings for convenience in describing the utility model and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the utility model.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," "secured," and the like are to be construed broadly and can, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.

In the present invention, unless otherwise expressly stated or limited, the first feature "on" or "under" the second feature may be directly contacting the first and second features or indirectly contacting the first and second features through an intermediate. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Referring to fig. 1 to 4, fig. 1 is a schematic structural view illustrating a PCR plate according to an embodiment of the present invention, fig. 2 is a front view of the PCR plate shown in fig. 1, fig. 3 is a cross-sectional view of the PCR plate shown in fig. 2 taken along a direction a-a, and fig. 4 is a side view of the PCR plate shown in fig. 2. An embodiment of the present invention provides a PCR plate, which includes a plate body 10. The plate body 10 has a bottom surface 11 and a top surface 12 facing away from the bottom surface 11. The top surface 12 is recessed inwardly to form a plurality of sample wells 121 for holding a sample liquid. That is, one end of each sample well 121 facing the top surface 12 is opened through the top surface 12, so as to facilitate the sample loading and sampling operations of the sample wells 121; the end of each sample hole 121 facing the bottom surface 11 is a closed end, so as to prevent the sample liquid in the sample hole 121 from leaking from the bottom surface 11.

In practical use, the PCR plate is subjected to nucleic acid extraction and purification to form a sample solution, and the sample solution is transferred into the sample well 121 and stored in a storage environment. When amplification detection is needed, the PCR plate is arranged on the PCR detector, the temperature control part on the PCR detector is in heat conduction contact with the bottom surface 11 and the top surface 12 of the PCR plate, heat exchange is carried out between the sample liquid in each sample hole 121 and the temperature control part through the bottom surface 11 and the top surface 12 of the plate body 10, accordingly, the temperature of the sample liquid in each sample hole 121 is controlled (heated or cooled) through the temperature control part, the heat conduction area is large, the temperature of the sample liquid in the sample hole 121 can be rapidly controlled within a required temperature range, the temperature rise and fall efficiency is favorably improved, and the detection efficiency is further improved.

In particular, in the embodiment, the top surface 12 and the bottom surface 11 of the plate body 10 are both used for thermal contact with a temperature control member, so that the temperature of the sample liquid in each sample well 121 is controlled by the temperature control member. Preferably, the top surface 12 and the bottom surface 11 are both flat surfaces, so as to be more convenient for fully contacting with the temperature control member, improve the heat transfer efficiency (i.e. the temperature rise and fall efficiency), and further improve the detection efficiency. Of course, in other embodiments, the bottom surface 11 and the top surface 12 may also be curved, which is beneficial to increase the contact area with the temperature control element, thereby improving the heat transfer efficiency.

In specific embodiments, the cross-sectional shape of the sample hole 121 may be circular, oval, diamond, or rectangular, and the like, and is not limited herein. Preferably, the cross-sectional shape of the sample well 121 is circular.

In one embodiment, the PCR plate further comprises a plurality of annular protrusions 20 protruding from the top surface 12, the plurality of annular protrusions 20 correspond to the plurality of sample holes 121 one by one, and each annular protrusion 20 is disposed around a corresponding one of the sample holes 121. In this way, each sample hole is surrounded by one annular protrusion 20, and the annular protrusion 20 can prevent contaminants (e.g. sample liquid dripping on the top surface 12) falling on the top surface 12 from entering the sample hole 121 through the opening, so that the contamination of the sample liquid in the sample hole 121 is avoided, and the accuracy of amplification detection is improved. It should be noted that the height of the annular protrusion 20 protruding from the top surface 12 can be designed according to practical situations, and is not limited herein.

Further, the plate body 10 and the plurality of protrusions are integrally formed, which is beneficial to simplifying the manufacturing process and reducing the manufacturing cost. Alternatively, the plate body 10 is integrally formed with the plurality of protrusions through an injection molding process.

In particular, in the embodiment, the PCR plate further comprises a cover detachably covering the top surface 12 of the plate body 10 to close the opening of each sample well 121 for storage.

In one embodiment, the cover member may be a cover plate, and is disposed on the top surface 12 of the plate body 10 by a cover plate cover to close the opening of each sample well 121. Further, the cover plate is provided with a plurality of fitting portions corresponding one-to-one to the plurality of annular protrusions 20. When the cover plate is covered on the top surface 12 of the body, each matching part is in sealing matching with a corresponding annular bulge 20. Therefore, on one hand, the matching part and the annular bulge 20 are utilized to position the cover plate, so that the cover plate can be covered in place and is convenient to use; on the other hand, the opening of the sample hole 121 is sealed by the matching part and the annular bulge 20, so that the sealing effect is improved. Alternatively, when the cover plate is covered on the top surface 12 of the body, each of the engaging portions is disposed on the outer side or the inner side of the corresponding one of the annular protrusions 20.

In another embodiment, the cover member may be a cover film covering the top surface 12 of the plate body 10 to close the opening of each sample well 121. When the sealing is not needed, the covering film is uncovered.

Referring to fig. 1, 5 to 7, in the embodiment of the present invention, the sample wells 121 on the top surface 12 are arranged in an array, so as to facilitate amplification detection with a PCR detector, for example, facilitate transfer of samples in each sample well 121 of a previous row to each sample well 121 of a next row. Preferably, the plurality of sample wells 121 are uniformly arranged on the top surface 12 of the plate body 10.

Referring to fig. 1, in one embodiment, the number of the sample holes 121 on the top surface 12 is 8, and the 8 sample holes 121 are spaced apart along the longitudinal direction of the plate body 10. That is, the 8 sample wells 121 of the top surface 12 are arranged in a row.

Referring to fig. 5, in another embodiment, the number of the sample holes 121 on the top surface 12 is 24, and the 24 sample holes 121 are arranged in 3 rows and 8 columns. That is, 24 sample wells 121 are arranged in 3 rows, and 8 sample wells 121 are arranged at intervals in each row.

Referring to fig. 6, in another embodiment, the number of the sample holes 121 on the top surface 12 is 48, and the 48 sample holes 121 are arranged in 6 rows and 8 columns. That is, the 48 sample holes 121 are arranged in 6 rows, and 8 sample holes 121 are arranged at intervals in each row.

Referring to fig. 7, in another embodiment, the number of the sample wells 121 on the top surface 12 is 96, and the 96 sample wells 121 are arranged in 12 rows and 8 columns. That is, 96 sample wells 121 are arranged in 12 rows, and 8 sample wells 121 are arranged at intervals in each row.

It should be noted that, of course, the number of the sample wells 121 of the PCR plate may be other number specifications, and is not limited herein.

Referring to fig. 8 to 10, based on the PCR plate 100, an embodiment of the utility model further provides a fluorescence PCR detector, which includes a housing 200, a fluorescence detection mechanism 300, and the PCR plate 100 in any of the embodiments. The fluorescence detection mechanism 300 and the PCR plate 100 are both disposed in the housing 200, and the fluorescence detection mechanism 300 is used for performing fluorescence PCR detection on the sample solution in each sample hole 121 on the PCR plate 100. It should be noted that one PCR plate 100 may be mounted on the casing 200, or two or more PCR plates 100 may be mounted thereon, which is not limited herein.

Specifically, the PCR plate 100 is mounted on the housing 200, and the top surface 12 of the PCR plate 100 is located outside the housing 200, and the bottom surface 11 of the PCR plate 100 is located inside the housing 200. The fluorescence PCR detector further includes a cover plate 400 and a first temperature control module (not shown), wherein the cover plate 400 is configured to cover the top surface 12 of the PCR plate 100, and the first temperature control module is disposed on the cover plate 400. In this way, when the cover plate 400 covers the top surface 12 of the PCR plate 100, the heat generated by the first temperature control module is transferred from the top surface 12 of the PCR plate 100 to the sample solution in the sample wells 121, so as to control the temperature of the sample solution. Optionally, one end of the cover plate 400 is rotatably connected to the housing 200. The cover plate 400 covers the top surface 12 of the PCR plate 100 during rotation relative to the housing 200.

Further, the fluorescence PCR detector further includes a second temperature control module 500 disposed in the casing 200, and the second temperature control module 500 is attached to the bottom surface 11 of the PCR plate 100. The second temperature control module 500 has a plurality of exposure holes (not shown) formed in one-to-one correspondence with the plurality of sample holes 121. The fluorescence detection mechanism 300 performs fluorescence PCR detection on the sample solution in each corresponding sample well 121 through each exposed well. Thus, the temperature of the sample solution in each sample hole 121 is controlled by the first temperature control module and the second temperature control module 500 through the top surface 12 and the bottom surface 11 of the PCR plate 100, which is beneficial to improving the temperature control efficiency and the temperature control precision.

All possible combinations of the technical features of the above embodiments may not be described for the sake of brevity, but should be considered as within the scope of the present disclosure as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent should be subject to the appended claims.

Claims (10)

1. A PCR plate, comprising a plate body (10), said plate body (10) having a bottom surface (11) and a top surface (12) facing away from said bottom surface (11), said top surface (12) being recessed inwardly to form a plurality of sample wells (121) for holding a sample fluid.

2. The PCR plate of claim 1, further comprising a plurality of annular protrusions (20) protruding from the top surface (12), wherein the plurality of annular protrusions (20) correspond to the plurality of sample wells (121) in a one-to-one manner, and each of the annular protrusions (20) is arranged around a corresponding one of the sample wells (121).

3. The PCR plate of claim 2, wherein the plate body (10) is integrally formed with the plurality of annular projections (20).

4. PCR plate according to claim 1, wherein the top face (12) and the bottom face (11) are both adapted for thermal contact with a temperature control element.

5. The PCR plate of claim 1, wherein the plurality of sample wells (121) are arranged in an array.

6. The PCR plate of claim 5, wherein the number of the sample wells (121) is 8, and 8 of the sample wells (121) are arranged at intervals in the longitudinal direction of the plate body (10); or

The number of the sample holes (121) is 24, and the 24 sample holes (121) are arranged in 3 rows and 8 columns; or

The number of the sample holes (121) is 48, and the 48 sample holes (121) are arranged in 6 rows and 8 columns; or

The number of the sample holes (121) is 96, and the 96 sample holes (121) are arranged in 12 rows and 8 columns.

7. The PCR plate of claim 1 further comprising a cover removably covering the top surface (12) of the plate body (10).

8. A fluorescent PCR detector, comprising a housing (200), a fluorescent detection mechanism (300) and a PCR plate (100) according to any one of claims 1 to 7;

the fluorescence detection mechanism (300) and the PCR plate (100) are both arranged on the shell (200), and the fluorescence detection mechanism (300) is used for carrying out fluorescence PCR detection on the sample liquid in each sample hole (121) on the PCR plate (100).

9. The fluorescent PCR detector of claim 8, wherein the top surface (12) of the PCR plate (100) is located outside the housing (200) and the bottom surface (11) of the PCR plate (100) is located inside the housing (200);

the fluorescent PCR detector further comprises a cover plate (400) and a first temperature control module, wherein the cover plate (400) is configured to cover the top surface (12) of the PCR plate (100), and the first temperature control module is arranged on the cover plate (400).

10. The fluorescent PCR detector of claim 9, further comprising a second temperature control module (500) disposed within the housing (400), the second temperature control module (500) being attached to the bottom surface (11) of the PCR plate (100);

the second temperature control module (500) has a plurality of exposure holes corresponding to the plurality of sample holes (121), and the fluorescence detection mechanism (300) performs fluorescence PCR detection on the sample liquid in each sample hole (121) through each exposure hole.

Images