CN214183232U - PCR system configuration EP pipe support - Google Patents

Abstract

The utility model relates to a PCR system disposes EP pipe support, including last body pipe support and the lower part of the body ice box of setting in last body pipe support lower part, last body pipe support includes the support body, and one side system has a plurality of reagents to place the slotted hole on the support body, and opposite side array has a plurality of PCR pipes to place the hole, every PCR pipe places all still to make apron groove, every on the support body of hole one side PCR pipe is placed the notch department in apron groove all is equipped with spacing rotatory protruding groove admittedly, every the bottom in apron groove all is provided with push type lever mechanism, and the vertical activity cartridge in apron groove has the closure lid temporarily, temporarily closes a lid bottom an organic whole system pivot temporarily, place on push type lever mechanism apron tank bottom, drive push type lever mechanism realizes the closure lid temporarily and bounces, and rotatory closure lid realizes that PCR pipe upper portion is temporarily sealed. The utility model discloses simple structure, modern design through the design of closing the lid temporarily, the evaporation of moisture in the reducible trace system makes the experiment system configuration more accurate, has improved the efficiency of experiment greatly.

Description

PCR system configuration EP pipe support

Technical Field

The utility model belongs to the technical field of experimental apparatus, a EP pipe support is related to, especially a PCR system configuration EP pipe support.

Background

In the experimental project, a commonly used molecular detection means is a PCR amplification technology, PCR is widely applied to the research of gene expression due to the advantages of specificity, sensitivity, high efficiency, rapidness, good repeatability and the like, a plurality of small EP tubes are generally arranged and placed on an EP tube frame in order in the RNA reverse transcription and Q-PCR experimental process, and are added with samples one by one, because the number of the EP tubes is large and small, the added reagent is trace, each tube cover cannot be closed in time and rapidly, water in the reagent can be evaporated, because of the trace reagent, concentration change can have great influence on the experimental result, a plurality of reagents are added, too much time is wasted when the cover is closed each time, the experimental repeatability is not good, the intervals of the small EP tube frames used in the experiment are small, and the side tubes are easy to be damaged when the cover is closed or the cover is opened or the side tubes are easy to be knocked away in the taking process; therefore, an EP tube rack which can realize temporary cover closing and reduce the evaporation of water in the reagent is urgently needed.

No patent documents relevant to the present application were found by search.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art's weak point, provide the evaporation of moisture in the reducible micro-system, make experiment system configuration more accurate, and be difficult for bumping miniature EP pipe more in operation process, dispose the EP pipe support to the PCR system that reduces the waste of experiment reagent and time.

The utility model provides a its technical problem take following technical scheme to realize:

an EP pipe frame for PCR system configuration comprises an upper body pipe frame and a lower body ice box arranged at the lower part of the upper body pipe frame, the upper body pipe frame comprises a frame body, one side of the upper surface of the frame body is a reagent placing area, a plurality of reagent placing slotted holes are uniformly distributed and arranged at intervals, the other side of the upper surface of the frame body is a PCR pipe placing area, a plurality of PCR pipe placing holes are uniformly distributed and arrayed at intervals, a cover plate groove is also formed in the frame body at one side of each PCR pipe placing hole, a limiting rotary protruding groove is fixedly arranged at the groove opening of each cover plate groove, a pressing type lever mechanism is arranged at the bottom of each cover plate groove, a temporary cover is vertically and movably inserted in each cover plate groove, the bottom of the temporary closing cover is provided with a rotating shaft matched with the limiting rotary protruding groove, the bottom of the cover plate groove is placed on the pressing type lever mechanism to drive the pressing type lever mechanism, the temporary closing cover is bounced, and the temporary closing cover is rotated to temporarily seal the upper part of the PCR tube.

And, push type lever mechanism includes lever base, balancing pole, reset spring, presses the depression bar and presses the button 4, lever base adorns admittedly and keeps away from the bottom that PCR pipe placed hole one side at the apron groove, and a balancing pole through the pivot hinge dress balancing pole on the lever base, can place the closure temporarily on the balancing pole one end that corresponds the apron groove position, and the balancing pole lower extreme of this end then is fixed mutually through reset spring and apron groove bottom, realizes spacingly, and the balancing pole other end is the quarter butt, quarter butt upper surface vertical solid dress presses the depression bar, press sliding installation in depression bar and the support body, and press the upper surface that the depression bar upper end stretches out in the support body.

And, every the coaxial system in press the depression bar top has a press button, the instruction label of the PCR pipe of corresponding adding reagent is posted on the press button.

And, the upper surface of the lower part of the body ice box is evenly provided with through holes at intervals.

And a low-temperature channel is formed inside the PCR tube placing hole and the upper tube frame.

Moreover, a pinching groove opening is integrally formed in the top of the temporary closing cover.

The utility model has the advantages that:

the utility model has the advantages of simple structure and novel design, through the design that closes the lid temporarily, be applicable to placing of system configuration PCR pipe in RNA reverse transcription and the PCR amplification experiment, the evaporation of moisture in the reducible micro-system makes the experiment system configuration more accurate, owing to have closed the protection of lid temporarily in addition, be difficult for bumping miniature EP pipe in operation process more, reduced the waste of experimental reagent, saved the experimentation time.

The utility model discloses overall structure comprises upper part of the body pipe support and lower part of the body ice box two parts, is used for placing the ice that has made in the lower part of the body ice box for the experiment is gone on ice, guarantees best experimental condition, has improved experimental efficiency greatly.

Drawings

Fig. 1 is a schematic top view of the present invention;

FIG. 2 is a schematic cross-sectional view of FIG. 1A-A.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.

A PCR system configuration EP pipe support comprises an upper body pipe support 6 and a lower body ice box 15 arranged at the lower part of the upper body pipe support, wherein the upper body pipe support comprises a support body, a reagent placing area 2 and a PCR pipe placing area, one side of the support body is the reagent placing area, a plurality of reagent placing slotted holes 1 are uniformly distributed and spaced on the upper surface of the reagent placing area and used for placing reagent bottles in a limiting way, the other side of the support body is the PCR pipe placing area, a plurality of PCR pipe placing holes 3 are uniformly distributed and spaced and arrayed on the upper surface of the placing area, a cover plate groove is also formed on the upper surface of the PCR pipe placing area at one side of each PCR pipe placing hole, a limiting rotary convex groove 10 is fixedly arranged at the position of each groove, a pressing type lever mechanism is arranged at the bottom of each cover plate groove, a temporary cover 5 which can assist in covering the PCR pipes is vertically movably inserted on the pressing type lever mechanism, temporarily close a pivot 11 of an organic whole system in lid bottom, pivot and spacing rotatory protruding inslot looks adaptation drive push type lever mechanism, realize temporarily closing the lid and bounce to insert the pivot through manual in the spacing rotatory protruding inslot, then rotatory temporarily close the lid and cover at the PCR pipe, realize that PCR pipe upper portion is sealed temporarily, prevent that the evaporation of experimental reagent is extravagant.

The push type lever mechanism comprises a lever base 7, a balance rod 8, a reset spring 12, a press rod 9 and a press button 4, wherein the lever base is fixedly arranged at the bottom of one side of a cover plate groove far away from a PCR tube placing hole, the lever base is hinged with the balance rod through a rotating shaft, a temporary closing cover can be placed at one end of the balance rod corresponding to the cover plate groove, the lower end of the balance rod at the end is fixed with the bottom of the cover plate groove through the reset spring to realize limiting, the other end of the balance rod is a short rod, the upper surface of the short rod is vertically and fixedly provided with the press rod, the press rod and a frame body are arranged in a sliding mode, the upper end of the press rod extends out of the upper surface of the frame body, the press rod is coaxially arranged at the top end of the press rod, an indication mark corresponding to the PCR tube added with a reagent is pasted on the press button, the press button is pressed down, the temporary closing cover arranged in the cover plate groove can be bounced, and then manually drawing out the temporary closing cover and rotating the cover on the PCR tube to which the reagent is added.

In the specific implementation of the utility model, in order to make the lower body ice box provide low temperature for the upper body pipe frame, through holes 14 are uniformly arranged on the upper surface of the lower body ice box at intervals;

furthermore, a low-temperature channel 13 is formed in the PCR tube placing hole and the upper body pipe frame, so that the low-temperature gas of the lower body ice box can surround the PCR tube placing hole, and the whole experiment can be carried out on ice.

The utility model discloses in the implementation, in order to experiment operating personnel to more conveniently take the temporary lid that is bounced still an organic whole system has one to hold between the fingers notch 16 on the temporary lid top.

This device is made by the duroplasts, and overall structure comprises upper part of the body pipe support and lower part of the body ice box two parts, is used for placing the ice that makes in the lower part of the body ice box, and the hole is used for placing the enzyme of configuration system is placed to the reagent, reagents such as primer, and the hole is placed to the PCR pipe and is used for placing little PCR pipe, the utility model discloses when concrete operation, when one or more reagent has been put to the PCR pipe, when another PCR pipe need be placed, in order to prevent that the intraductal reagent of former PCR from volatilizing or turning over, press the button that it corresponds, will arrange the temporarily that the apron inslot closes the lid and bounce in, then with the hand seize rotatory lid can on the PCR pipe, when need add the reagent to this PCR pipe again, only need will temporarily close the lid take and insert the apron inslot can.

The utility model has the advantages of simple structure and novel design, through the design that closes the lid temporarily, the evaporation of moisture in the reducible trace system makes the experiment system configuration more accurate, owing to have closed the protection of lid temporarily, is difficult for bumping miniature EP pipe in operation process more moreover, has reduced the waste of experimental reagent, has saved the experimental time.

The utility model discloses overall structure comprises upper part of the body pipe support and lower part of the body ice box two parts, is used for placing the ice that has made in the lower part of the body ice box for the experiment is gone on ice, guarantees best experimental condition, has improved experimental efficiency greatly.

Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.

Claims (6)

1. An EP pipe support is configured in a PCR system, and is characterized in that: comprises an upper body pipe frame and a lower body ice box arranged at the lower part of the upper body pipe frame, the upper body pipe frame comprises a frame body, one side of the upper surface of the frame body is provided with a reagent placing area, a plurality of reagent placing slotted holes are uniformly distributed and arranged at intervals, the other side of the upper surface of the frame body is provided with a PCR tube placing area, a plurality of PCR tube placing holes are uniformly distributed and arranged at intervals, a cover plate groove is also arranged on the frame body at one side of each PCR tube placing hole, a limiting rotary protruding groove is fixedly arranged at the groove mouth of each cover plate groove, the bottom of each cover plate groove is provided with a pressing type lever mechanism, a temporary cover is vertically and movably inserted in each cover plate groove, the bottom of the temporary closing cover is provided with a rotating shaft matched with the limiting rotary protruding groove, the bottom of the cover plate groove is placed on the pressing type lever mechanism to drive the pressing type lever mechanism, the temporary closing cover is bounced, and the temporary closing cover is rotated to temporarily seal the upper part of the PCR tube.

2. The PCR system configured EP pipe rack according to claim 1, characterized in that: push type lever mechanism includes lever base, balancing pole, reset spring, presses the depression bar and presses button (4), lever base adorns admittedly and keeps away from the bottom that PCR pipe placed hole one side at the apron groove, a balancing pole through the pivot hinge dress on the lever base, can place the closure temporarily on the balancing pole one end that corresponds the apron trench position, and the balancing pole lower extreme of this end then is fixed mutually through reset spring and apron groove bottom, realizes spacingly, and the balancing pole other end is the quarter butt, quarter butt upper surface vertical solid dress presses the depression bar, press slidable mounting in depression bar and the support body, and stretch out in the upper surface of support body according to the depression bar upper end.

3. The PCR system configured EP pipe rack according to claim 2, characterized in that: every the coaxial system in press the depression bar top has a press button, paste on the press button and have the instruction label that corresponds the PCR pipe that adds the reagent.

4. The PCR system configured EP pipe rack according to claim 1, characterized in that: the upper surface of the lower ice box is uniformly provided with through holes at intervals.

5. The PCR system configured EP pipe rack according to claim 1, characterized in that: the PCR tube placing hole and the inner part of the upper tube frame are provided with a low-temperature channel.

6. The PCR system configured EP pipe rack according to claim 1, characterized in that: the top of the temporary closing cover is also integrally provided with a pinching notch.

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