CN219546947U

CN219546947U - Pressurizer and shrouding device that perforated plate sealed usefulness

Info

Publication number: CN219546947U
Application number: CN202320841604.3U
Authority: CN
Inventors: 赵斯斯; 薛茜
Original assignee: SANGON BIOTECH (SHANGHAI) CO Ltd
Current assignee: SANGON BIOTECH (SHANGHAI) CO Ltd
Priority date: 2023-04-14
Filing date: 2023-04-14
Publication date: 2023-08-18
Anticipated expiration: 2033-04-14

Abstract

The utility model provides a pressurizer and a sealing plate device for sealing a porous plate, and relates to the technical field of PCR consumables. It comprises the following steps: the movable device and the fixing device are matched with each other, the fixing device comprises a base and a limiting part arranged at the top of the base, and a cavity for accommodating the porous plate is formed in the base; the limiting part is provided with a movable hole for the movable device to move and circumferentially limit, the limiting part is communicated with the cavity of the base, the movable device comprises a movable rod, one end of the movable rod extends inwards to the cavity of the base along the limiting part, and a pressing plate is fixedly connected to the end part of the movable rod in the cavity. Still provide a shrouding device, promoted the efficiency of shrouding to a great extent.

Description

Pressurizer and shrouding device that perforated plate sealed usefulness

Technical Field

The utility model relates to the technical field of PCR consumables, in particular to a pressurizer and a sealing plate device for sealing a porous plate.

Background

The polymerase chain reaction (Polymerase chain reaction, PCR) is a molecular biology technique used for in vitro amplification of specific DNA fragments and is extremely widely used in molecular biology. Fluorescent quantitative PCR is a method of measuring the total amount of a product after each Polymerase Chain Reaction (PCR) cycle using a fluorescent chemical in a DNA amplification reaction. And quantitatively analyzing the specific DNA sequence in the sample to be tested by an internal reference method or an external reference method. Fluorescent quantitative PCR is not only a molecular biological experimental means, but also a common method for clinical laboratory.

Wherein, 8 are connected with the pipe, 12 are connected with the pipe, 96 orifice plates, 384 orifice plates are the common consumptive material of PCR, and use with its corresponding tube cap and sealing membrane combination. Taking 96 well plates as an example, in most cases, the 96 well plates are combined with sealing films, and meanwhile, a rectangular hard plastic scraping plate is needed, and the operation flow of the sealing plate is as follows: (1) adding a sample to a 96-well plate; (2) Tearing off the surface layer of the sealing film, and sealing one surface with the adhesive on the 96-well plate; (3) The seal film is compacted on the 96-well plate by a hard plastic scraper, and each gap is compacted by the scraper. The third step is time-consuming and labor-consuming, and is not conducive to a large number of operations and analyses.

In view of this, the present utility model has been made.

Disclosure of Invention

The utility model aims to provide a pressurizer for sealing a porous plate and a sealing plate device so as to improve the efficiency of the sealing plate.

The utility model is realized in the following way:

in a first aspect, the present utility model provides a pressurizer for sealing a porous plate, comprising: the movable device and the fixing device are matched with each other, the fixing device comprises a base and a limiting part arranged at the top of the base, and a cavity for accommodating the porous plate is formed in the base; the limiting part is provided with a movable hole for the movable device to move and circumferentially limit, the limiting part is communicated with the cavity of the base, the movable device comprises a movable rod, one end of the movable rod extends inwards into the cavity of the base along the limiting part, and a pressing plate is fixedly connected to the end part of the movable rod in the cavity;

the movable device is further provided with a first lifting part at one end far away from the base.

The inventor provides a pressurizer, which is matched with a fixing device through a movable device to realize quick gland, and is suitable for quick labor-saving sealing of a porous plate, such as a porous plate, a 48-well plate, a 96-well plate or a 384-well plate which is formed by 8-pipe joints and/or at least one group of 12-pipe joints. The first lifting part is lifted and held to enable the movable device to move up and down in the movable hole of the fixing device, and the pressing plate is driven to move up and down, so that the porous plate is sealed by the pressing plate.

In a preferred embodiment of the application of the utility model, the base is provided with openings on at least one side for placing the perforated plates. This facilitates placement of the perforated plate and placement of the plate into the cavity in the base.

The openings are arranged on the side surfaces, so that the porous plate can be conveniently taken and placed at a plurality of positions. For example, it may be provided to place the porous plate from one direction and to remove the porous plate from the other, opposite direction.

In a preferred embodiment of the application of the utility model, the base is provided with openings on one side or on two opposite sides for placing the perforated plates.

In a preferred embodiment of the present utility model, the first grip portion extends outward beyond the limiting portion, and a movable gap is left between the bottom of the grip portion and the limiting portion.

The first lifting and holding part is convenient to press, and the clamping hand can be prevented by extending to the outside of the limiting part. The movable gap can be adaptively adjusted according to the distance between the pressing plate of the movable device and the bottom end of the inner chamber of the base.

In a preferred embodiment of the present utility model, the first grip portion is a grip plate, a handle, a grip ball or a grip lever.

In the preferred embodiment of the utility model, one end of the limiting part far away from the base is fixedly provided with a second lifting part; and the base and the limiting part are integrally formed.

In a preferred embodiment of the present utility model, the second grip portion is a grip plate, a handle or a grip lever. The first lifting part and the second lifting part are arranged to be beneficial to saving labor, and when the novel lifting device is used, both hands simultaneously lift the first lifting part and the second lifting part, and the labor is saved compared with the process of independently lifting the first lifting part.

In a preferred embodiment of the present utility model, the movable device in the movable hole is in a column, cuboid or cube shape. So long as it can cooperate with the movable hole, it is within the inventive concept of the present utility model.

In a second aspect, the utility model also provides a sealing plate device for sealing a porous plate, which comprises the pressurizer for sealing the porous plate and a placing plate, wherein the placing plate is provided with a groove for placing the porous plate.

The groove has the effect of limiting the clamping connection of the PCR tube, and prevents the porous plate from sliding in the sealing process of the pressing plate.

In a preferred embodiment of the utility model, the multi-well plate is a multi-well plate, 48-well plate, 96-well plate or 384-well plate of at least one set of 8-well tubes and/or at least one set of 12-well tubes, and the dimensions of the placement plate are smaller than the dimensions of the chambers of the pressurizer.

In other embodiments, multiwell plates include, but are not limited to, a 96-well plate after clipping or a 384-well plate after clipping.

Compared with the prior art, the utility model has the beneficial effects that:

the utility model provides a pressurizer, which is matched with a fixing device through a movable device to realize quick gland, and is suitable for quick labor-saving sealing of a porous plate, such as a porous plate, a 48-well plate, a 96-well plate, a 192-well plate or a 384-well plate of 8-well pipes and/or at least one group of 12-well pipes. The first lifting part is lifted and held to enable the movable device to move up and down in the movable hole of the fixing device, and the pressing plate is driven to move up and down, so that the porous plate is sealed by the pressing plate.

In addition, still provide a shrouding device, this shrouding device can satisfy the swift sealed demand of the perforated plate of multiple model.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present utility model, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present utility model and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic perspective view of a pressurizer;

FIG. 2 is a front cross-sectional view of the pressurizer;

fig. 3 is a schematic perspective view of a placement plate of the sealing plate device.

Icon: 1-grooves; 2-placing a plate; 3-a first grip plate; 4-a movable rod; 5-a second grip plate; 6, a base; 7-pressing plates; 8-limit part.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations.

Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", "clockwise", "counterclockwise", and the like indicate an azimuth or a positional relationship based on that shown in the drawings, or an azimuth or a positional relationship in which the inventive product is conventionally put in use, are merely for convenience of describing the present utility model and for simplifying the description, and do not indicate or imply that the apparatus or element to be referred to must have a specific azimuth, be constructed and operated in a specific azimuth, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

Furthermore, the terms "horizontal," "vertical," "overhang," and the like do not denote a requirement that the component be absolutely horizontal or overhang, but rather may be slightly inclined. As "horizontal" merely means that its direction is more horizontal than "vertical", and does not mean that the structure must be perfectly horizontal, but may be slightly inclined.

In the description of the present utility model, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present utility model will be understood in specific cases by those of ordinary skill in the art.

Example 1

Referring to fig. 1 and 2, the present embodiment provides a pressurizer, which includes a limiting portion 8, a base 6 and a movable device, wherein the limiting portion 8 specifically includes a rectangular limiting portion 8 body and a second lifting plate 5 disposed above the limiting portion 8 body. The cuboid-shaped limiting part 8 body is provided with a movable hole for the movable device to move up and down. The base 6 is rectangular and has openings on both sides, and the base 6 has a cavity therein for mounting a platen 7 of the movable device and a perforated plate (not shown). By pressing the movable device, the quick sealing plate is realized.

Specifically, the movable device comprises a first lifting plate 3 (fixedly connected with one end of the movable rod 4) far away from the base 6, the movable rod 4 and a pressing plate 7 fixedly connected with the other end of the movable rod 4. In this embodiment, the movable rod 4 of the first lifting plate 3 and the pressing plate 7 are integrally formed.

A movable gap is reserved between the bottom of the first lifting and holding part and the limiting part 8, and the movable gap is reserved between the bottom of the first lifting and holding part and the second lifting and holding plate 5 as shown in the figure 1 so as to meet the requirement of the space for the movable rod 4 to move up and down during pressurization.

The movable rod 4 extends inwards to the cavity of the base 6 along the limiting part 8, and the pressing plate 7 is positioned in the cavity of the base 6. The base 6 is provided with openings on at least one side to place a perforated plate. The two opposite sides of this embodiment are open.

For convenience in processing and production, the second lifting plate 5, the base 6 and the limiting part 8 are integrally formed.

Referring to fig. 1 and 3, the present embodiment further provides a sealing plate device for sealing a porous plate, which includes the pressurizer for sealing a porous plate and a placing plate 2, and a groove 1 for placing the porous plate is formed in the placing plate 2. The number of grooves 1 is, for example, 48, 96, 192 or 384, and can be adjusted as required.

The placement plate 2 is arranged as a rectangular whole and is integrated with the groove 1 as a whole, and has a hard plastic texture. The bottom of the base 6 is a hard material, for example a metallic material.

The using method comprises the following steps:

the 96-well plate to be sealed is placed on the placement plate 2 in fig. 3, and the PCR tube is placed in the groove 1 correspondingly. The seal film was aligned over the 96-well plate. Then, the placing plate 2 containing the 96-well plate is placed in the cavity of the base 6 in fig. 1, and the first lifting and holding part and the second lifting and holding part are simultaneously held, so that the bottom of the pressing plate 7 is tightly attached to the upper surface of the sealing film of the 96-well plate. In other embodiments, in order to better attach the 96-well plate to each gap of the sealing film, the bottom surface (the surface opposite to the groove 1) of the other placing plate 2 may be used alone to press and attach the 96-well plate attached with the sealing film, so that the 96-well plate and the sealing film remain sealed.

Example 2

This embodiment differs from embodiment 1 in that the porous plate to be sealed is different. This example is a multi-well plate with 3 12-pipe assemblies. In other embodiments, the perforated plates to be sealed may also be perforated plates consisting of other numbers of 8-tube or 12-tube.

The using method comprises the following steps:

the 3 12-way tubes to be sealed are placed on the placement plate 2 in fig. 3, and the PCR tubes are placed in the grooves 1 correspondingly. Tube caps were aligned over 3 12-up tubes, respectively. The placing plate 2 is then placed in the cavity of the base 6 of fig. 1, and the first lifting part and the second lifting part are simultaneously gripped, so that the tube cover is tightly attached to the pressing plate 7, and each gap between the 96-well plate and the tube cover is tightly attached. The placement plate 2 in this embodiment serves to fix 12 the wall of the union.

The above is only a preferred embodiment of the present utility model, and is not intended to limit the present utility model, but various modifications and variations can be made to the present utility model by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present utility model should be included in the protection scope of the present utility model.

Claims

1. A pressurizer for sealing a porous plate, comprising: the movable device and the fixing device are matched with each other, the fixing device comprises a base and a limiting part arranged at the top of the base, and a cavity for accommodating the porous plate is formed in the base; the limiting part is provided with a movable hole for the movable device to move and circumferentially limit, the limiting part is communicated with the cavity of the base, the movable device comprises a movable rod, one end of the movable rod extends inwards into the cavity of the base along the limiting part, and a pressing plate is fixedly connected to the end part of the movable rod in the cavity;

2. The pressurizer for sealing a porous plate according to claim 1, wherein said base is provided with an opening on at least one side for placing a porous plate.

3. The pressurizer for sealing a porous plate according to claim 2, wherein the base is provided with openings on one side or two opposite sides for placing the porous plate.

4. The pressurizer for sealing a porous plate according to claim 1, wherein the first lifting part extends outwards beyond the limiting part, and a movable gap is left between the bottom of the first lifting part and the limiting part.

5. The pressurizer for sealing a porous plate according to claim 4, wherein said first grip part is a grip plate, a handle, a grip ball or a grip lever.

6. The pressurizer for sealing a porous plate according to claim 4, wherein a second lifting part is fixedly arranged at one end of the limiting part far away from the base; and the base and the limiting part are integrally formed.

7. The pressurizer for sealing a porous plate according to claim 6, wherein said second lifting and holding part is a holding plate, a handle or a lifting and holding rod.

8. The pressurizer for sealing a porous plate according to claim 1, wherein said movable means located in said movable hole has a columnar shape.

9. A closing plate device for sealing a porous plate, characterized in that it comprises the pressurizer for sealing a porous plate according to any one of claims 1-8 and a placing plate provided with a groove for placing a porous plate.

10. The sealing plate device for sealing a porous plate according to claim 9, wherein the porous plate is at least one group of 8-connected tubes and/or at least one group of 12-connected tubes, 48-, 96-or 384-well plates, and the size of the placement plate is smaller than the size of the chamber of the pressurizer.