CN214986944U

CN214986944U - Nucleic acid extraction kit

Info

Publication number: CN214986944U
Application number: CN202121290963.1U
Authority: CN
Inventors: 张志鹏; 陶金程; 王雷; 张志强
Original assignee: Beijing Applied Biological Technologies Co ltd
Current assignee: Beijing Applied Biological Technologies Co ltd
Priority date: 2021-06-09
Filing date: 2021-06-09
Publication date: 2021-12-03
Anticipated expiration: 2031-06-09

Abstract

The utility model provides a nucleic acid extraction kit, which comprises a box body and a box cover; the reagent box comprises a reagent rack and a plurality of reagent grooves, wherein the reagent rack is transversely provided with a plurality of rows of reagent grooves, and each row of reagent grooves is provided with at least one reagent groove; the box cover comprises a cover plate and baffles, and a plurality of baffles are arranged above the cover plate; a plurality of openings are arranged on the cover plate in the interval of the baffle plate; when the box cover is combined with the box body, one opening corresponds to one row of reagent grooves, and the baffle is positioned between each row of reagent grooves. The method is suitable for extracting nucleic acid with large flux, and saves cost; avoiding cross-contamination between each column of reagent wells.

Description

Nucleic acid extraction kit

Technical Field

The utility model relates to a nucleic acid detects technical field, especially relates to a nucleic acid extraction kit.

Background

Nucleic acid extraction is an essential link in various molecular biology experiments, and almost every laboratory requires nucleic acid extraction for separation and purification of biomolecules. Along with the rapid development of society, the demand of large-flux nucleic acid extraction is gradually increased, most of nucleic acid extraction kits in the current market are designed in a single strip, the single strip is very troublesome for large-flux detection, the operation is complex, and the anti-pollution measure is not good enough.

Chinese patent CN209024499U discloses a high-throughput nucleic acid extraction kit, which comprises magnetic beads, a microporous plate cover matched with the microporous plate and a main electromagnetic chuck detachably arranged at the bottom of the microporous plate; the microplate comprises a plurality of wells; the pore wall of each micropore protrudes out of a first connecting area between each micropore and the adjacent micropore and a second connecting area between each micropore and the side wall of the microporous plate; the microporous plate cover is provided with an annular bulge matched with the inner diameter of the micropore; the microplate and the microplate cover are arranged to be placed into the PCR instrument to replace the PCR tube and the PCR rack. The kit can directly add PCR related reagents after extracting nucleic acid by using the microporous plate, covers the microporous plate, and then is put into a PCR instrument for PCR amplification reaction without transferring into a PCR tube, thereby avoiding the loss of a nucleic acid template and the pollution of the nucleic acid and simplifying the operation steps.

The anti-pollution kit in the prior art has a complex structure and is inconvenient to use. Therefore, it is highly desirable to design a nucleic acid extraction kit that is easier and faster to handle and that is resistant to contamination.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems existing in the prior art, the utility model provides a nucleic acid extraction kit which can be suitable for the extraction of nucleic acid with large flux and can prevent the cross contamination of nucleic acid.

In order to achieve the above purpose, the technical scheme of the utility model is as follows:

a nucleic acid extraction kit comprises a kit body and a kit cover; the reagent box comprises a reagent rack and a plurality of reagent grooves, wherein the reagent rack is transversely provided with a plurality of rows of reagent grooves, and each row of reagent grooves is provided with at least one reagent groove; the box cover comprises a cover plate and baffles, and a plurality of baffles are arranged above the cover plate; a plurality of openings are arranged on the cover plate in the interval of the baffle plate; when the box cover is combined with the box body, one opening corresponds to one row of reagent grooves, and the baffle is positioned between each row of reagent grooves.

Further, the reagent tank is in an elliptic cylinder shape.

Furthermore, each row of reagent grooves of the box body is provided with a transition groove.

Further, the upper surfaces of the reagent tanks are higher than the upper surface of the reagent rack, and the upper surfaces of all the reagent tanks are flush.

Furthermore, both sides of the cover plate are provided with side wing clamping plates, and the side wing clamping plates are perpendicular to the cover plate and extend downwards.

Furthermore, a plurality of circular arc grooves are formed in two sides of the reagent rack, a plurality of circular arc protrusions are correspondingly formed in the inner sides of the side wing clamping plates, and when the box cover is combined with the box body, the protrusions are matched with the grooves.

Further, the baffle is perpendicular to the cover plate.

Further, the number of the baffles is the same as the number of rows of the reagent wells.

The utility model has the advantages that:

1. the reagent grooves are arranged in multiple rows, so that the nucleic acid extraction kit is suitable for extracting nucleic acid with large flux, the efficiency is improved, and the cost is saved;

2. the box cover is provided with a baffle plate to avoid cross contamination among each row of reagent tanks;

3. through the cooperation between the recess of box body and the arch of lid, do benefit to the fixed of lid position, the cooperation between reinforcing box body and the lid, it is also more convenient to separate between box body and the lid.

Drawings

FIG. 1 is a front view of the cassette of the nucleic acid extraction kit of the present invention.

Fig. 2 is a top view of the cassette shown in fig. 1.

FIG. 3 is an isometric view of the lid of the nucleic acid extraction kit provided by the present invention.

Fig. 4 is a bottom view of the cap shown in fig. 3.

FIG. 5 is a schematic structural diagram of the nucleic acid extraction kit provided by the present invention.

In the figure, the reagent box comprises a reagent rack 1, a reagent box 2, a reagent groove 3, a cover plate 4, a baffle plate 5, an opening 6, a transition groove 7, a side wing clamping plate 8, a groove 9 and a protrusion.

Detailed Description

The technical solution of the present invention will be described clearly with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

It should be noted that the terms "center", "upper", "lower", "horizontal", "left", "right", "lateral", "longitudinal", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus should not be construed as limiting the present invention.

As shown in FIGS. 1-5, the utility model provides a nucleic acid extraction kit, which comprises a box body and a box cover; the reagent box comprises a reagent rack 1 and a plurality of reagent grooves 2, wherein six rows of reagent grooves 2 are transversely arranged on the reagent rack 1, and each row of reagent grooves 2 comprises seven reagent grooves 2; the box cover comprises a cover plate 3 and baffles 4, and a plurality of baffles 4 are arranged above the cover plate 3; a plurality of openings 5 are arranged on the cover plate 3 in the interval of the baffle plate 4; when the box cover is combined with the box body, one opening 5 corresponds to one row of reagent grooves 2, and the baffle plate 4 is positioned between each row of reagent grooves 2.

Further, the reagent tank 2 has an elliptical cylindrical shape.

Furthermore, each row of reagent grooves 2 of the box body is provided with a transition groove 6, so that the magnet in the nucleic acid extraction equipment can conveniently ascend and descend.

Further, the upper surface in reagent groove 2 is higher than the upper surface of reagent frame 1, and all the upper surface parallel and level of reagent groove 2, after the kit finishes using, paste the membrane that seals that prevents the reagent liquid and spill over on reagent groove 2 upper surface, the kit of being convenient for is retrieved.

Further, both sides of the cover plate 3 are provided with a side wing clamping plate 7, and the side wing clamping plate 7 is perpendicular to the cover plate 3 and extends downwards.

Furthermore, a plurality of circular arc grooves 8 are formed in two sides of the reagent rack 1, the grooves 8 are symmetrically distributed on two sides of the reagent rack 1, a plurality of circular arc protrusions 9 are correspondingly arranged on the inner sides of the side wing clamping plates 7, and when the box cover is combined with the box body, the protrusions 9 are matched with the grooves 8.

Further, the baffle 4 is perpendicular to the cover plate 3.

Further, the number of the baffles 4 is the same as the number of rows of the reagent wells 2.

The utility model is provided with the reagent grooves which are arranged in multiple rows, which is suitable for extracting nucleic acid with large flux, improves the efficiency and saves the labor cost; the box cover is provided with a baffle 4 to avoid cross contamination among each row of reagent tanks 2; set up circular arc recess 8 on the box body, set up circular arc arch 9 on the lid, through the cooperation between recess 8 of box body and the arch 9 of lid, do benefit to the fixed of lid position to reinforcing the degree of cooperation between box body and the lid, it is also more convenient to separate between box body and the lid.

The above embodiments are only used to illustrate the technical solutions of the present invention and not to limit, although the present invention has been described in detail with reference to the examples, those skilled in the art should understand that the technical solutions of the present invention can be modified or replaced by equivalents without departing from the scope of the technical solutions of the present invention, and all of them should be covered in the scope of the claims of the present invention.

Claims

1. A nucleic acid extraction kit comprises a kit body and a kit cover; the method is characterized in that: the reagent box comprises a reagent rack and a plurality of reagent grooves, wherein the reagent rack is transversely provided with a plurality of rows of reagent grooves, and each row of reagent grooves is provided with at least one reagent groove; the box cover comprises a cover plate and baffles, and a plurality of baffles are arranged above the cover plate; a plurality of openings are arranged on the cover plate in the interval of the baffle plate; when the box cover is combined with the box body, one opening corresponds to one row of reagent grooves, and the baffle is positioned between each row of reagent grooves.

2. The nucleic acid extraction kit according to claim 1, wherein: the reagent tank is in an elliptic cylinder shape.

3. The nucleic acid extraction kit according to claim 1, wherein: and transition grooves are formed in each row of reagent grooves of the box body.

4. The nucleic acid extraction kit according to claim 1, wherein: the upper surface of the reagent tank is higher than the upper surface of the reagent rack, and the upper surfaces of all the reagent tanks are flush.

5. The nucleic acid extraction kit according to claim 1, wherein: and two sides of the cover plate are provided with side wing clamping plates, and the side wing clamping plates are vertical to the cover plate and extend downwards.

6. The nucleic acid extraction kit according to claim 5, wherein: the reagent shelf is characterized in that a plurality of arc grooves are formed in two sides of the reagent shelf, a plurality of arc protrusions are correspondingly formed in the inner sides of the side wing clamping plates, and when the box cover is combined with the box body, the protrusions are matched with the grooves.

7. The nucleic acid extraction kit according to claim 1, wherein: the baffle is perpendicular to the cover plate.

8. The nucleic acid extraction kit according to claim 1, wherein: the number of the baffles is the same as the number of the rows of the reagent tanks.