CN216427262U - Nucleic acid extraction box - Google Patents
Nucleic acid extraction box Download PDFInfo
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- CN216427262U CN216427262U CN202023329948.1U CN202023329948U CN216427262U CN 216427262 U CN216427262 U CN 216427262U CN 202023329948 U CN202023329948 U CN 202023329948U CN 216427262 U CN216427262 U CN 216427262U
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Abstract
The utility model discloses a nucleic acid extraction box, which comprises a box body, wherein the box body is sequentially provided with a first tip storage area for placing a small tip, a first reagent storage area, a second tip storage area for placing a second tip, a second reagent storage area and a reaction area from left to right, and the height of the first tip is smaller than that of the second tip; the first reagent storage area and the second reagent storage area respectively comprise a plurality of reagent bins which are arranged at intervals, and reagent sealing films are arranged at the tops of the reagent bins; the reaction zone comprises a reaction bin with an upward vertical opening, and the bottom of the reaction bin body is an arc-shaped vortex mixing structure; the first gun head storage area, the first reagent storage area, the second gun head storage area, the second reagent storage area and the reaction area are connected into a whole through reinforcing ribs. The puncture device, the storage tube and the tube cover placing area are not arranged, the volume of the nucleic acid extraction box is reduced, the nucleic acid extraction box is prevented from falling off in the loading process, the puncture device is reused in the pretreatment process, and the material cost is reduced.
Description
Technical Field
The utility model relates to the technical field of in-vitro diagnosis, in particular to a nucleic acid extraction kit.
Background
The gene amplification technology (PCR) is a molecular biology technology for amplifying and amplifying specific DNA, and can specifically amplify millions of times of trace target DNA, thereby greatly improving the analysis and detection capability of DNA molecules.
As a pretreatment procedure of PCR amplification reaction, the quality of the PCR purification process directly affects the effect of the PCR amplification reaction.
The nucleic acid extraction box is a disposable consumable used in the PCR purification process, can contain various reaction reagents and provides a reaction space. The reaction bin of the existing nucleic acid extraction box is provided with the puncture device, the puncture device is easy to fall off in the process of loading the nucleic acid extraction box, and the puncture device is disposable and high in material consumption cost; reagent in the reaction bin is not mixed uniformly and takes long time, which affects purification efficiency.
In view of the above, how to reduce the cost of nucleic acid extraction consumables and improve the purification efficiency is a problem that needs to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention provides a nucleic acid extraction cassette, which effectively reduces the cost of consumables for nucleic acid extraction and improves the purification efficiency.
In order to achieve the above purpose, the utility model provides the following technical scheme:
a nucleic acid extraction box comprises a box body, wherein a first tip storage area for placing a first tip, a first reagent storage area, a second tip storage area for placing a second tip, a second reagent storage area and a reaction area are sequentially arranged on the box body from left to right, and the height of the first tip is smaller than that of the second tip;
the first reagent storage area and the second reagent storage area respectively comprise a plurality of reagent bins which are arranged at intervals, and reagent sealing films are arranged at the tops of the reagent bins;
the reaction zone comprises a reaction bin with an upward vertical opening, and the bottom of the reaction bin body is an arc-shaped vortex mixing structure;
the first lance head storage area, the first reagent storage area, the second lance head storage area, the second reagent storage area and the reaction area are connected into a whole through reinforcing ribs.
Preferably, the reagent storehouse is square reagent storehouse, just the bottom in reagent storehouse is equipped with the back taper structure.
Preferably, the first reagent storage area is sequentially provided with a first reagent small bin, a first reagent large bin, a second reagent small bin and three second reagent large bins from left to right, the heights of the second reagent small bins are the same as those of the first reagent small bins, and the heights of the second reagent large bins are the same as those of the first reagent large bins.
Preferably, the second reagent storage area is sequentially provided with a third reagent large bin and a third reagent small bin from left to right, the height of the third reagent large bin is the same as that of the first reagent large bin, and the height of the third reagent small bin is the same as that of the first reagent small bin.
Preferably, the inner contour line of the upper opening of the chamber of the reaction chamber is a kidney-shaped structure consisting of a straight line section and an arc section, a main body surface and a heating surface are arranged on the chamber from top to bottom at the position corresponding to the straight line section, a reagent enrichment surface used for being connected with an enrichment device is arranged on the chamber at the position corresponding to the arc section, and the included angle between the reagent enrichment surface and the axis of the second gun head storage area is 0-10 degrees.
Preferably, the inner wall surface of the bin at the position corresponding to the reagent enrichment surface is provided with a liquid injection surface for contacting with a liquid sucker, the bottom of the bin is provided with a liquid vortex surface and a liquid buffer surface from bottom to top on the inner wall surface of the bin, and the liquid vortex surface, the liquid buffer surface and the liquid injection surface form the vortex uniformly-mixing structure.
The utility model provides a nucleic acid extraction box which comprises a box body, wherein the box body is sequentially provided with a first tip storage area for placing a small tip, a first reagent storage area, a second tip storage area for placing a second tip, a second reagent storage area and a reaction area from left to right, and the height of the first tip is smaller than that of the second tip; the first reagent storage area and the second reagent storage area respectively comprise a plurality of reagent bins which are arranged at intervals, and reagent sealing films are arranged at the tops of the reagent bins; the reaction zone comprises a reaction bin with an upward vertical opening, and the bottom of the reaction bin body is an arc-shaped vortex mixing structure; the first gun head storage area, the first reagent storage area, the second gun head storage area, the second reagent storage area and the reaction area are connected into a whole through reinforcing ribs.
When in use, the reagent sealing film on the nucleic acid extraction box is removed, and the nucleic acid extraction box is loaded in the nucleic acid extraction instrument; after the nucleic acid extractor works, pricking a second tip head in a second gun head storage area by using a tip adapter on the nucleic acid extractor, and adding a sample, a magnetic bead reagent and a reagent required by reaction into the reaction bin at one time; the bottom of the reaction bin body is of a vortex mixing structure, and the magnetic bead reagent and the reaction reagent are fully premixed in a vortex mixing mode; the nucleic acid extractor heats the reaction bin to provide an incubation environment; after the reaction is finished, adsorbing the magnetic bead reagent by using an enrichment device, transferring the reacted reaction reagent into a reagent bin, removing the enrichment device, adding an elution reagent into the reaction bin by using a second tip head, and dissolving the target DNA on the magnetic bead reagent into the elution reagent; and pricking the first tip head by using a tip adapter to transfer the purified target DNA into the PCR tube, and sealing the PCR tube by using a tube cover.
Because the reaction zone does not contain the puncture device and the storage tube and the tube cover placing zone are cancelled, the volume of the nucleic acid extraction box is reduced, thereby reducing the volume of a nucleic acid extraction box loading system on the nucleic acid extraction instrument, avoiding the nucleic acid extraction box from falling off in the loading process, realizing the reuse of the puncture device in the pretreatment process and reducing the material cost.
Meanwhile, the vortex mixing structure in the reaction bin is beneficial to the full mixing reaction of the reagent, and the purification efficiency of the target DNA is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an embodiment of a nucleic acid extraction cassette according to the present invention;
FIG. 2 is a schematic sectional view of the nucleic acid extraction cassette in FIG. 1 in a front view direction;
FIG. 3 is a schematic structural view of the reaction chamber in FIG. 1;
fig. 4 is a schematic sectional view of the reaction chamber in fig. 3 in a front view direction.
In fig. 1-4:
01 is a first tip storage area, 02 is a first reagent storage area, 03 is a second tip storage area, 04 is a second reagent storage area, 05 is a reaction area, 1 is a reaction bin, 11 is a main body surface, 12 is a heating surface, 13 is a reagent enrichment surface, 14 is an injection liquid surface, 15 is a liquid vortex surface, 16 is a liquid buffer surface, 2 is a reagent small bin, 3 is a reagent large bin, 4 is a second tip head, 5 is a first tip head, 6 is a reagent sealing film, and 7 is a reinforcing rib.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The core of the utility model is to provide a nucleic acid extraction box, which effectively reduces the material cost of nucleic acid extraction and improves the purification efficiency.
Referring to FIGS. 1-4, FIG. 1 is a schematic structural diagram of a nucleic acid extraction cassette according to an embodiment of the present invention; FIG. 2 is a schematic sectional view of the nucleic acid extraction cassette in FIG. 1 in a front view direction; FIG. 3 is a schematic structural view of the reaction chamber in FIG. 1; fig. 4 is a schematic sectional view of the reaction chamber in fig. 3 in a front view direction.
Note that the height direction mentioned in this document is an extending direction of the reagent cartridge, and the length direction is a direction perpendicular to the height direction in the paper.
The nucleic acid extraction box provided by the utility model comprises a box body, wherein a first tip storage area 01 for placing a small tip head 5, a first reagent storage area 02, a second tip storage area 03 for placing a second tip head 4, a second reagent storage area 04 and a reaction area 05 are sequentially arranged on the box body from left to right, and the height of the first tip head 5 is smaller than that of the second tip head 4; the first reagent storage area 02 and the second reagent storage area 04 both comprise a plurality of reagent bins which are arranged at intervals, and reagent sealing films 6 are arranged at the tops of the reagent bins; the reaction zone 05 comprises a reaction bin 1 with an upward vertical opening, and the bottom of the bin body of the reaction bin 1 is an arc-shaped vortex mixing structure; the first lance head storage area 01, the first reagent storage area 02, the second lance head storage area 03, the second reagent storage area 04 and the reaction area 05 are connected into a whole through reinforcing ribs 7.
Referring to fig. 1, two ends of the box body in the length direction are provided with guide slots, and the structures of the guide slots and other parts of the box body are referred to in the prior art and are not described herein again.
A first tip head 5 is arranged in the first tip head storage area 01, a second tip head 4 is arranged in the second tip head storage area 03, and the sizes of the first tip head storage area 01 and the second tip head storage area 03 are determined according to the diameter and the length of the tip head. It should be noted that, in order to avoid the gun head from being polluted during the repeated use, a certain gap needs to be reserved at the head of the gun head in both the first gun head storage area 01 and the second gun head storage area 03.
The first reagent storage area 02 and the second reagent storage area 04 are used for storing reaction reagents required in the PCR purification process, and preferably, in order to avoid polluting unused consumables in the using process, the reagents in the first reagent storage area 02 and the second reagent storage area 04 are distributed on the left side of the reaction area 05 from the near to the far according to the using sequence. The height of each reagent cabin is determined according to the reagent sealing amount and the puncture height of the reagent cabin.
Preferably, the reagent cabin can be set as a square reagent cabin, and the bottom of the reagent cabin is provided with an inverted cone structure. The square reagent bin is beneficial to keeping the distance between the inner wall of the reagent bin and the tip head consistent when the tip head sucks the reagent, and the reagent storage capacity is increased; the inverted cone structure at the bottom can collect the reagent at the bottom of the reagent bin, so that the reagent sealing amount is reduced, and the reagent waste is avoided.
Referring to fig. 1 and 2, preferably, the first reagent storage area 02 is sequentially provided with a first small reagent bin, a first large reagent bin, a second small reagent bin and three second large reagent bins from left to right, wherein the height of the second small reagent bin is the same as that of the first small reagent bin, and the height of the second large reagent bin is the same as that of the first large reagent bin.
The first reagent small bin and the second reagent small bin are reagent small bins 2, the first reagent large bin and the second reagent large bin are reagent large bins 3, and the specific height of the reagent small bins 2 and the specific height of the reagent large bins 3 need to be determined according to the required reagent sealing amount in actual production.
Preferably, the second reagent storage area 04 is provided with a third reagent large bin and a third reagent small bin in sequence from left to right, the height of the third reagent large bin is the same as that of the first reagent large bin, and the height of the third reagent small bin is the same as that of the first reagent small bin. The height of the reagent small bin 2 and the height of the reagent large bin 3 are uniformly arranged, and compared with the height difference of each reagent bin, the structure of the nucleic acid extraction box is favorably simplified, and the production and the manufacture of the nucleic acid extraction box are facilitated.
When in use, the reagent sealing film 6 on the nucleic acid extraction box is removed, and the nucleic acid extraction box is loaded in the nucleic acid extraction instrument; after the nucleic acid extractor works, a tip adapter on the nucleic acid extractor is used for pricking a second tip head 4 in a second gun head storage area 03, and a sample, a magnetic bead reagent and a reagent required by reaction are added into the reaction bin 1 at one time; the bottom of the reaction bin 1 is a vortex mixing structure, and the magnetic bead reagent and the reaction reagent are fully premixed in a vortex mixing mode; the nucleic acid extractor heats the reaction chamber 1 to provide an incubation environment; after the reaction is finished, adsorbing the magnetic bead reagent by using an enrichment device, transferring the reacted reaction reagent into a reagent bin, removing the enrichment device, adding an elution reagent into the reaction bin 1 by using a second tip head 4, and dissolving the target DNA on the magnetic bead reagent into the elution reagent; and pricking the first tip head 5 by using a tip adapter to transfer the purified target DNA into the PCR tube, and sealing the PCR tube by using a tube cover.
It should be noted that, the steps of adding reagents, premixing, incubating, enriching and eluting in the above process may be one time or multiple times, and the specific times need to be determined according to the needs of the actual PCR detection.
In this embodiment, because do not contain the puncture ware in the reaction zone 05 and cancelled and deposited pipe and tube cap and place the district, reduced the volume of nucleic acid extraction box to reduced nucleic acid extraction box loading system's on the nucleic acid extraction appearance volume, avoided nucleic acid extraction box to drop in the loading process, still realized the used repeatedly of puncture ware among the pretreatment process, reduced the consumptive material cost.
Meanwhile, the vortex mixing structure in the reaction bin 1 is beneficial to the full mixing reaction of the reagents, and the purification efficiency of the target DNA is improved.
On the basis of the above embodiment, the structure of the reaction chamber 1 is limited, the inner contour line of the upper opening of the chamber of the reaction chamber 1 is a kidney-shaped structure composed of a straight line segment and a circular arc segment, the chamber at the position corresponding to the straight line segment is provided with a main body surface 11 and a heating surface 12 from top to bottom, the chamber at the position corresponding to the circular arc segment is provided with a reagent enrichment surface 13 used for being connected with an enrichment device, and the included angle between the reagent enrichment surface 13 and the axis of the second gun head storage area 03 is 0-10 °.
The heating surface 12 is in contact with an incubation device of the nucleic acid extractor to heat the reagent in the reaction chamber 1 by heat transfer, and preferably, the heating surface 12 is a smooth plane to increase the heat flux per unit time by increasing the contact area, thereby improving the heating efficiency.
The reagent enrichment surface 13 is used for connecting and contacting with an enrichment device, please refer to fig. 2 and fig. 4, the reagent enrichment surface 13 is disposed at the right side of the reaction chamber 1 to provide sufficient installation space for the enrichment device.
The reagent enrichment surface 13 is arranged approximately vertically to reduce the risk of reagent wall hanging during the enrichment process.
Preferably, the inner wall surface of the bin at the position corresponding to the reagent enrichment surface 13 is provided with a liquid injection surface 14 for contacting with a liquid sucker, the inner wall surface of the bin at the bottom of the bin is provided with a liquid vortex surface 15 and a liquid buffer surface 16 from bottom to top, and the liquid vortex surface 15, the liquid buffer surface 16 and the liquid injection surface 14 form a vortex mixing structure.
When the liquid sucker injects liquid, the reagent flows into the bottom of the bin from the liquid injection surface 14, and due to the arc vortex mixing structure, the reagent rises to the liquid buffer surface 16 along the upward vortex of the liquid vortex surface 15, and then falls into the bottom of the bin again under the action of self gravity, so that premixing of the reagent and the magnetic bead reagent is realized, the reagent and the magnetic bead reagent are in full contact, and amplification of target DNA under a subsequent incubation environment is facilitated.
It should be noted that, in this document, reference is made to the first tip head 5 and the second tip head 4, the first lance tip storage area 01 and the second lance tip storage area 03, the first reagent storage area 02 and the second reagent storage area 04, the first reagent silo, the second reagent silo, and the third reagent silo, and the first, the second, and the third of the first reagent silo, the second reagent silo, and the third reagent silo, which are used only for distinguishing the difference of positions, and do not include any limitation on the sequence.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other.
The nucleic acid extraction cassette provided by the present invention is described in detail above. The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (6)
1. A nucleic acid extraction box is characterized by comprising a box body, wherein a first tip storage area (01) for placing a first tip (5), a first reagent storage area (02), a second tip storage area (03) for placing a second tip (4), a second reagent storage area (04) and a reaction area (05) are sequentially arranged on the box body from left to right, and the height of the first tip (5) is smaller than that of the second tip (4);
the first reagent storage area (02) and the second reagent storage area (04) respectively comprise a plurality of reagent bins which are arranged at intervals, and reagent sealing films (6) are arranged at the tops of the reagent bins;
the reaction zone (05) comprises a reaction bin (1) which is vertically and upwards opened and is communicated with the external environment, and the bottom of the bin body of the reaction bin (1) is of an arc-shaped vortex mixing structure;
the second tip head (4) is used for matching with a tip adapter of a nucleic acid extractor to add samples and reagents into the reaction bin (1), and the first tip head (5) is used for matching with the tip adapter to transfer purified target DNA in the reaction bin (1) into a PCR tube;
the first lance head storage area (01), the first reagent storage area (02), the second lance head storage area (03), the second reagent storage area (04) and the reaction area (05) are connected into a whole through reinforcing ribs (7).
2. The nucleic acid extraction cassette according to claim 1, wherein the reagent well is a square reagent well, and an inverted cone structure is provided at the bottom of the reagent well.
3. The nucleic acid extraction cassette according to claim 2, wherein the first reagent storage area (02) is provided with a first reagent small chamber, a first reagent large chamber, a second reagent small chamber and three second reagent large chambers in this order from left to right, the height of the second reagent small chamber is the same as the height of the first reagent small chamber, and the height of the second reagent large chamber is the same as the height of the first reagent large chamber.
4. The nucleic acid isolation cassette according to claim 3, wherein a third reagent large chamber and a third reagent small chamber are provided in the second reagent storage region (04) in this order from left to right, the height of the third reagent large chamber is the same as the height of the first reagent large chamber, and the height of the third reagent small chamber is the same as the height of the first reagent small chamber.
5. The nucleic acid extraction cassette according to any one of claims 1 to 4, wherein an inner contour line of an opening in the chamber of the reaction chamber (1) is a kidney-shaped structure consisting of a straight line section and a circular arc section, a main body surface (11) and a heating surface (12) are arranged on the chamber from top to bottom at a position corresponding to the straight line section, a reagent enrichment surface (13) for connecting with an enrichment device is arranged on the chamber at a position corresponding to the circular arc section, and an included angle between the reagent enrichment surface (13) and an axis of the second lance tip storage area (03) is 0-10 degrees.
6. The nucleic acid extraction cassette according to claim 5, wherein an injection surface (14) for contacting a liquid aspirator is provided on an inner wall surface of the chamber at a position corresponding to the reagent enrichment surface (13), a liquid vortex surface (15) and a liquid buffer surface (16) are provided on an inner wall surface of the chamber at a bottom of the chamber from bottom to top, and the liquid vortex surface (15), the liquid buffer surface (16) and the injection surface (14) constitute the vortex mixing structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023329948.1U CN216427262U (en) | 2020-12-29 | 2020-12-29 | Nucleic acid extraction box |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023329948.1U CN216427262U (en) | 2020-12-29 | 2020-12-29 | Nucleic acid extraction box |
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| CN216427262U true CN216427262U (en) | 2022-05-03 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625870A (en) * | 2020-12-29 | 2021-04-09 | 安图实验仪器(郑州)有限公司 | Nucleic acid extraction box |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112625870A (en) * | 2020-12-29 | 2021-04-09 | 安图实验仪器(郑州)有限公司 | Nucleic acid extraction box |
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