CN215591391U

CN215591391U - PCR kit

Info

Publication number: CN215591391U
Application number: CN202121290964.6U
Authority: CN
Inventors: 唐清泰; 杨海英; 王雷; 张志强
Original assignee: Beijing Applied Biological Technologies Co ltd
Current assignee: Beijing Applied Biological Technologies Co ltd
Priority date: 2021-06-09
Filing date: 2021-06-09
Publication date: 2022-01-21
Anticipated expiration: 2031-06-09

Abstract

The utility model provides a PCR kit, which comprises a kit body and a kit cover; the reagent box comprises a reagent rack and a plurality of reagent grooves, wherein a plurality of rows of the reagent grooves are transversely arranged on the reagent rack, and at least one reagent groove is longitudinally arranged in each row of the reagent grooves; the box cover comprises a cover plate and a plurality of pipe plugs, the pipe plugs are arranged at the bottom of the cover plate, and when the box cover is pressed into the box body, the pipe plugs and the reagent grooves are tightly combined together; the utility model can be suitable for a large amount of PCR detection, is convenient for the grabbing of the manipulator, prevents the cross contamination during the PCR detection and improves the accuracy of the PCR detection.

Description

PCR kit

Technical Field

The utility model relates to the technical field of PCR detection, in particular to a PCR kit.

Background

PCR is known as polymerase chain reaction, and is a method of using a piece of DNA as a template, and amplifying the piece of DNA to a sufficient amount under the participation of DNA polymerase and nucleotide substrate so as to analyze the structure and function. The PCR detection method has extremely important significance in clinic, and in order to accurately detect PCR components, the PCR components need to be ensured not to be polluted in the detection process.

Chinese patent CN207498363U discloses a PCR detection kit cover, which comprises a cover plate and a plurality of tube plugs, wherein the cover plate is provided with a plurality of ball-head cylinders, the tube plugs are provided with ball-head cylinder holes, and the ball-head cylinders are in interference fit with the ball-head cylinder holes to tightly combine the cover plate and the tube plugs together; when the box cover is matched with the reagent box for use, the tube plug can be plugged into a reagent tube of the reagent box. The PCR detection kit box cover is suitable for a biological detection technology workstation, facilitates the grabbing of a manipulator in the workstation, does not need manual operation, improves the working efficiency, can effectively avoid mutual pollution caused by the fact that reagents in various reagent tubes are heated, evaporated and polluted in the PCR detection process, and improves the accuracy of actual detection results.

The anti-pollution kit in the prior art is of an integrated structure and is inconvenient to use. Therefore, it is desirable to design a PCR kit that is simpler and faster to handle and that is resistant to contamination.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems of the prior art, the present invention provides a PCR kit which is suitable for high-throughput PCR detection and can prevent cross contamination of PCR reagents.

In order to achieve the purpose, the technical scheme of the utility model is as follows:

a PCR kit comprises a box body and a box cover; the reagent box comprises a reagent rack and a plurality of reagent grooves, wherein a plurality of rows of reagent grooves are transversely arranged on the reagent rack, and at least one reagent groove is longitudinally arranged in each row of reagent grooves; the lid includes apron and a plurality of stopcock, the stopcock sets up in the apron bottom, and when the lid box body was impressed, the stopcock with reagent groove closely combines together.

Further, the reagent groove is cylindrical, and the diameter of the horizontal section of the reagent groove is gradually increased from bottom to top.

Further, the longitudinal distance of the reagent grooves is smaller than the transverse distance of the reagent grooves.

Further, the upper surface of the reagent tank is higher than the upper surface of the reagent rack.

Furthermore, one side of the reagent rack is provided with a clamping point, so that the automatic operation of a manipulator is facilitated.

Furthermore, a chamfer is arranged at one corner of the reagent rack.

Furthermore, a reinforcing column is arranged in the cover plate, the reinforcing column is arranged in the center of the cover plate, and a plurality of reinforcing ribs are arranged on two sides of the reinforcing column.

Furthermore, the outer surface of the pipe plug is provided with at least one annular bulge, the lower end of the bulge is provided with a circular truncated cone, and the diameter of the lower end of the circular truncated cone is smaller than that of the upper end of the circular truncated cone.

The utility model has the beneficial effects that:

1. the PCR kit is provided with the reagent tanks in multiple rows, the number of the reagent tanks is increased by multiple times compared with the prior art, and the PCR kit is suitable for a large amount of PCR detection and saves the cost;

2. the clamping points are arranged, so that the manipulator can conveniently grab the workpiece, manual operation is not needed, and the working efficiency is improved;

3. through the tight combination of lid and box body, avoid PCR reagent cross contamination, improve the accuracy of PCR detection.

4. The box body is sealed through the box cover, a waste liquid pool does not need to be designed independently, and the box cover is automatically covered by a manipulator after the reagent box is used up, so that the condition that the waste reagent cannot pollute the environment can be guaranteed.

Drawings

FIG. 1 is a schematic structural diagram of a PCR kit provided by the present invention.

Fig. 2 is a front view of the cartridge.

Fig. 3 is an isometric view of the cassette shown in fig. 2.

Fig. 4 is a front view of the cover.

Figure 5 is an isometric view of the cap of figure 2.

In the figure, the reagent kit comprises a reagent rack 1, a reagent tank 2, a reagent trough 3, a clamping point 4, a chamfer 5, a cover plate 6, a tube plug 7, a reinforcing column 8, a reinforcing rib 9, a bulge 10 and a circular truncated cone.

Detailed Description

The technical solutions of the present invention will be described in detail with reference to the accompanying drawings, and it is obvious that the described embodiments are not all embodiments of the present invention, and all other embodiments obtained by those skilled in the art without any inventive work belong to the protection scope of the present invention.

It is to be understood that the terms "center," "upper," "lower," "horizontal," "left," "right," "lateral," "longitudinal," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the present invention and for the simplicity of description, and do not indicate or imply that the referenced devices or elements must be in a particular orientation, constructed and operated in a particular orientation, and thus are not to be considered as limiting the present invention.

As shown in FIGS. 1 to 5, the present invention provides a PCR kit comprising a case body and a case cover; the reagent box comprises a reagent rack 1 and a plurality of reagent grooves 2, six rows of reagent grooves 2 are transversely arranged on the reagent rack 1, and five reagent grooves 2 are longitudinally arranged on each row of reagent grooves 2; the lid includes apron 5 and five stopcocks 6, stopcock 6 sets up in 5 bottoms on the apron, when the lid impresses the box body, stopcock 6 with reagent groove 2 closely combines together.

Further, reagent groove 2 is cylindrical, the horizontal cross-section diameter of reagent groove 2 is from up crescent down, the machine-shaping of the box body of being convenient for.

Further, the longitudinal intervals of the reagent wells 2 are smaller than the lateral intervals of the reagent wells 2.

Furthermore, the upper surface of the reagent groove 2 is higher than the upper surface of the reagent rack 1, when the box cover is pressed into the box body, the box cover is in direct contact with the upper surface of the reagent groove 2 and is not in contact with the upper surface of the reagent rack 1, and the sealing of the reagent groove 2 is facilitated.

Furthermore, reagent frame 1 one side sets up centre gripping point 3, the centre gripping of the manipulator of being convenient for is favorable to automated operation.

Furthermore, a chamfer 4 is arranged at one corner of the reagent rack 1, so that the direction of the reagent box can be unified conveniently in the automatic operation process.

Furthermore, a reinforcing column 7 is arranged in the cover plate 5, the reinforcing column 7 is arranged in the center of the cover plate 5, and a plurality of reinforcing ribs 8 are arranged on two sides of the reinforcing column 7 to increase the clamping bearing capacity of the box cover.

Furthermore, an annular bulge 9 is arranged on the outer surface of the pipe plug 6, a circular truncated cone 10 is arranged at the lower end of the bulge 9, and the diameter of the lower end of the circular truncated cone 10 is smaller than that of the upper end of the circular truncated cone 10; when the box cover is pressed into the box body, the front end of the pipe plug 6 is easier to enter the reagent groove 2, which is more beneficial to the pressing of the box cover; when the reagent tank 2 is in a sealed state, the annular protrusion 9 contacts the inner surface of the reagent tank 2, thereby increasing the sealing performance of the reagent tank 2.

The utility model is provided with a plurality of reagent grooves in parallel, which can be suitable for a large amount of PCR detection and save labor cost; the box body is provided with the clamping points 3, so that the manipulator can conveniently grab the box body, and the automatic assembly line operation is facilitated; the box cover and the box body are tightly combined, so that cross contamination of PCR reagents is prevented, and the accuracy of PCR detection is improved; the box body is sealed through the box cover, a waste liquid pool does not need to be designed independently, and the box cover is automatically covered by a manipulator after the reagent box is used up, so that the condition that the waste reagent cannot pollute the environment can be guaranteed.

Although the present invention has been described in detail with reference to examples, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the scope of the present invention.

Claims

1. A PCR kit comprises a box body and a box cover; the method is characterized in that: the reagent box comprises a reagent rack and a plurality of reagent grooves, wherein a plurality of rows of the reagent grooves are transversely arranged on the reagent rack, and at least one reagent groove is longitudinally arranged in each row of the reagent grooves; the lid includes apron and a plurality of stopcock, the stopcock set up in the apron bottom, work as the lid is impressed when the box body, the stopcock with reagent groove closely combines together.

2. A PCR kit according to claim 1, wherein: the reagent groove is cylindrical, and the diameter of the horizontal section of the reagent groove is gradually increased from bottom to top.

3. A PCR kit according to claim 1, wherein: the longitudinal space of the reagent grooves is smaller than the transverse space of the reagent grooves.

4. A PCR kit according to claim 1, wherein: the upper surface of the reagent tank is higher than the upper surface of the reagent rack.

5. A PCR kit according to claim 1, wherein: one side of the reagent rack is provided with a clamping point.

6. A PCR kit according to claim 1, wherein: and one corner of the reagent rack is provided with a chamfer.

7. A PCR kit according to claim 1, wherein: the reinforced column is arranged in the cover plate and is arranged in the center of the cover plate, and a plurality of reinforcing ribs are arranged on two sides of the reinforced column.

8. A PCR kit according to claim 1, wherein: the pipe plug comprises a bulge and a circular truncated cone; the outer surface of the pipe plug is provided with at least one annular bulge, the lower end of the bulge is provided with a circular truncated cone, and the diameter of the lower end of the circular truncated cone is smaller than that of the upper end of the circular truncated cone.