CN211274712U

CN211274712U - Container is loaded with consumptive material integration to sample

Info

Publication number: CN211274712U
Application number: CN201921906098.1U
Authority: CN
Inventors: 樊文俊; 陈晨
Original assignee: Hezhuo Biotechnology Shanghai Co ltd
Current assignee: Hezhuo Biotechnology Shanghai Co ltd
Priority date: 2019-11-04
Filing date: 2019-11-04
Publication date: 2020-08-18
Anticipated expiration: 2029-11-04

Abstract

The application provides a sample and consumable integrated loading container, which comprises a sample placing area, a suction head placing area, a reagent tube placing area and a PCR test tube placing area, wherein the sample placing area is arranged in the middle of the container; the sample is placed the district and is equipped with one or a plurality of recesses of arranging side by side at interval, the bottom cavity of recess, one side and the container intercommunication setting of recess form one or more accommodation spaces that are used for placing the sample, the district is placed to the suction head, the PCR test tube is placed the district and the reagent pipe is placed the district and is equipped with one or more first hole sites respectively on ascending direction, and one or more first hole sites in every region are used for placing suction head device, PCR test tube and reagent pipe respectively. This application picks up required consumptive material an organic whole integration of cell in a container, makes operating personnel more convenient when the operation, improves the cell and picks up efficiency.

Description

Container is loaded with consumptive material integration to sample

Technical Field

The utility model belongs to the technical field of biological detection, especially relate to the loading container with sample and consumptive material integration in the cell picks up.

Background

When the existing cell picking instrument picks cells, a container for loading the cells and various consumables are needed. Generally, cells are placed on a cell carrier such as a slide, a culture dish, or the like to be picked up; then a sucker placed on the mechanical arm picks up the sucker; the cells to be picked up are stored in PCR tubes and the reagents required for the picking up are placed in the reagent tubes.

When the cell picker is operated, the cell carrier, the PCR test tube and the reagent tube are placed in a scattered manner, wherein the cell carrier is placed on the stage, and the PCR test tube and the reagent tube are placed on the cell picker or a certain position on the operation stage. In this case, the operator is inconvenienced by picking up the cells, and the cell picking efficiency is reduced.

SUMMERY OF THE UTILITY MODEL

To the shortcoming or the deficiency of the prior art, the to-be-solved technical problem of the utility model is to place the container integration of cell carrier and consumptive material, improve operator's cell and pick up efficiency.

In order to solve the technical problem, the utility model provides a sample and consumable integrated loading container, which comprises a sample placing area, a suction head placing area, a reagent tube placing area and a PCR test tube placing area, wherein the sample placing area is arranged in the middle of the container, the suction head placing area is positioned on the left side of the sample placing area, the PCR test tube placing area is positioned on the right side of the sample placing area, and the reagent tube placing area is positioned on the front side of the sample placing area; the sample placing area is provided with one or a plurality of grooves arranged in parallel at intervals, the bottoms of the grooves are hollow, one sides of the grooves are communicated with the container to form one or more accommodating spaces for placing samples, the suction head placing area, the PCR test tube placing area and the reagent tube placing area are respectively provided with one or more first hole sites in the upward direction, and the one or more first hole sites in each area are respectively used for placing a suction head device, a PCR test tube and a reagent tube.

Further, the suction head placing area, the PCR test tube placing area and the reagent tube placing area are respectively provided with a fixed base, the fixed base is detachably connected with the container, a second hole position corresponding to the first hole position is arranged on the fixed base, and the second hole position is aligned with the corresponding first hole position and is used for fixing the suction head device, the PCR test tube and the reagent tube.

Further, the fixed base of the suction head placing area is provided with one or more second hole sites with different inner diameters.

Further, the inner diameter of the second hole site is not less than the outer diameter of the placed sucker device, PCR test tube and reagent tube.

Further, a plurality of first hole sites in the suction head placing area are arranged in a row, a plurality of first hole sites in the PCR test tube placing area are arranged in a row, and a plurality of hole sites in the reagent tube placing area are arranged in a row.

Furthermore, guide rails are arranged on the left side and the right side of each accommodating space, and the samples slide into the accommodating spaces from the outside to the inside from one side, communicated with the groove, of the container body along the guide rails.

Furthermore, a spring piece is arranged above the guide rail and fixed on the accommodating space through a screw.

Further, the sample is a slide or a cell culture dish.

Furthermore, the container body is provided with different marks in the suction head placing area, the PCR test tube placing area and the reagent tube placing area.

Furthermore, the container body is made of metal.

Compared with the prior art, the method has the following beneficial effects:

the sample and the consumptive material integration that this technical scheme provided load the container and will place the district and locate the left side that the district was placed to the sample, and the PCR test tube is placed the district and is located the right side that the district was placed to the sample, and reagent pipe is placed the integrative integration in district from a container the inside, makes operating personnel more convenient when picking up the cell, saves operating time, improves the cell and picks up efficiency.

Drawings

Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:

FIG. 1: the structure schematic diagram of the integrated loading container for the samples and the consumables in one embodiment of the application;

FIG. 2: the top view of the fixed base in the suction head placing area in one embodiment of the application.

FIG. 3: the application is reagent pipe in one embodiment places fixed base top view of district.

FIG. 4: the top view of the fixing base of the PCR test tube placing area in one embodiment of the application.

A suction head placing area 1, a reagent tube placing area 2, a PCR test tube placing area 3, a sample placing area 4, a first hole position 5, an accommodating space 6, a guide rail 7, a screw 8, a suction head device fixing base 9, a reagent tube fixing base 10, a PCR test tube fixing base 11 and a second hole position 12;

Detailed Description

The conception, the specific structure and the technical effects of the present invention will be further described with reference to the accompanying drawings, so as to fully understand the objects, the features and the effects of the present invention.

In one embodiment of the present application, as shown in fig. 1, the present application provides an integrated sample and consumable loading container. The container is provided with: a consumable for picking up a sample-a suction head, a consumable for loading a sample-a slide or a culture dish; a consumable-PCR tube for storing the picked up sample; a reagent tube, a consumable material for storing a sucker cleaning reagent and collecting waste liquid, is divided into regions according to different functions, and a container is divided into a sucker placing region 1, a reagent tube placing region 2, a PCR test tube placing region 3 and a sample placing region 4. The sample placing area 4 is provided in the middle of the container, and the placing areas are provided in front of, on the left of, and on the right of the sample placing area 4, respectively, with reference to the position of the sample placing area 4. Specifically, the tip placement area 1 is located on the left side of the sample placement area 4, the PCR tube placement area 3 is located on the right side of the sample placement area 4, and the reagent tube placement area 2 is located on the front side of the sample placement area 4. The advantage of partitioning according to function is that the operator can quickly complete the cell picking process according to the cell picking process. The cell is placed in the middle of the container, the first step is to pick up the cell by using the sucker device positioned on the left side, the second step is to place the cell in the PCR test tube on the right side after the picking up is finished, and the third step is to place the waste liquid generated in the picking up process in the front reagent tube. The whole process is compact, errors are not easy to occur, and compared with the existing operation mode, the operation time is saved.

The sample placing area 4 is provided with one or a plurality of grooves arranged in parallel at intervals, the bottoms of the grooves are hollow, one sides of the grooves are communicated with the container, and one or a plurality of accommodating spaces 6 for placing samples are formed. In one embodiment, illustrated in FIG. 1, the sample placement area 4 has 3 wells for receiving 3 slides. In the present embodiment, the number of grooves in the sample-placing region 4 is not limited thereto. The bottom of the groove is hollow, so that cells on the glass slide can be observed conveniently.

The tip placement area 1, the reagent tube placement area 2 and the PCR test tube placement area 3 are respectively provided with one or more first hole sites 5 in the upward direction, as shown in an embodiment shown in FIG. 1, the tip placement area 1 is provided with 9 first hole sites 5 for placing a tip device; the reagent tube placing area 2 is provided with 9 first hole sites 5 for placing the reagent tubes, and the PCR test tube placing area 3 is provided with 18 first hole sites 5 for placing the PCR test tubes. Of course, the placement and number of the first holes 5 are not limited thereto.

Further, in this embodiment, it is preferable that the tip placement section 1, the reagent tube placement section 2, and the PCR tube placement section 3 are each provided with a fixing base. As shown in FIG. 2, a suction head device fixing base 9 is detachably connected with a corresponding suction head placing area 1 on a vessel, and a second hole site 12 is arranged on the suction head device fixing base 9. Referring to fig. 3, a reagent tube fixing base 10 is detachably connected to a corresponding reagent tube placing region 2 of a container, and a second hole site 12 is provided on the reagent tube fixing base 10. As shown in FIG. 4, the PCR tube fixing base 11 is detachably connected to the corresponding PCR tube placing area 3 on the PCR tube fixing base 11 and the container, and the second hole site 12 is provided on the PCR tube fixing base 11. The second hole sites 12 may be aligned with the first hole sites 5 of the corresponding placement area, such that the second hole sites 12 may be inserted into the first hole sites 5 for securing the tip device, the reagent tubes and the PCR tubes. Taking the tip device fixing base 9 shown in FIG. 2 as an example, 7 second holes 12 are provided on the tip device fixing base 9, and can be used for placing 7 tip devices. The sucker device is placed on the sucker device fixing base 9, so that 7 second hole positions 12 on the fixing base are aligned with any 7 continuous first hole positions 5 on the first hole positions 5, and the sucker device fixing base 9 is placed on the sucker placing area 1. In this embodiment, the suction head device fixing base 9 can be detachably connected to the corresponding suction head placing area 1 on the container in various manners such as clamping fixation, screw fixation, and the like. Because the suction head devices have different inner diameters, as a preferred embodiment, the inner diameters of the 7 second holes 12 on the suction head device fixing base 9 shown in fig. 1 are not completely consistent, so that the suction head devices with different inner diameters can be placed, and the suction head device has extremely strong practicability. Of course, the number of the second hole sites 12 in this embodiment or the drawings is only one case, and in this application, it is only necessary that the number of the second hole sites 12 is not more than the number of the first hole sites 5. In addition, the inner diameter of the second hole site 12 is larger than the outer diameters of the tip device, the PCR test tube and the reagent tube, so that the outer diameters of the tip device, the PCR test tube and the reagent tube can be smoothly placed in the second hole site 12.

As a preference, in order to maximize the utilization of the capacity of the vessel, the plurality of first well sites 5 of the tip placement section 1 are arranged in a row, the plurality of first well sites 5 of the reagent tube placement section 2 are arranged in a row, and the plurality of first well sites 5 of the PCR tube placement section 3 are arranged in a column. As shown in FIG. 1, the 9 first holes 5 in the tip placement area 1 are arranged in 1 row; the 8 hole positions of the reagent tube placing area 2 are set to be 1 row; the 18 first well sites 5 of the PCR tube placing section 3 were set to 2 columns of 9 each. Of course, the first holes 5 of the tip placing region 1 and the PCR tube placing region 3 can be arranged in multiple rows, and the first holes 5 of the reagent tube placing region 2 can also be arranged in multiple rows according to the requirements in actual situations.

As shown in fig. 1, guide rails 7 are disposed on the left and right sides of each accommodating space 6, so that a sample can slide into the accommodating space 6 from the outside to the inside along the guide rails 7 from the side where the container body is communicated with the groove, and the sample can be well placed in the accommodating space. In this embodiment, the sample may be a slide or a petri dish in which the cells are placed. In addition, a spring piece is arranged above the guide rail 7 and fixed on the accommodating space 6 through a screw 8, and downward pressure is applied to the glass slide or the culture dish, so that the glass slide or the culture dish can be firmly fixed, and cells can be picked up conveniently.

As a preferable embodiment of this embodiment, the vessel body is distinguished by being provided with different labels in the tip placement region 1, the reagent vessel placement region 2 and the PCR vessel placement region 3. For example, the tip unit fixing base 9, the reagent vessel fixing base 10 and the PCR tube fixing base 11 may be provided in different colors for distinction, or may be distinguished by printing different patterns on the tip placement section 1, the reagent vessel placement section 2 and the PCR tube placement section 3. In addition, according to the different inner diameters of the suction head devices, marks can be made on the second holes 12 of the suction head placement area 1 to distinguish the suction head devices with different inner diameters.

In a preferred embodiment of the present invention, the container body is made of metal, and is wear-resistant and highly practical.

The above embodiments are merely provided to illustrate the technical solution of the present invention, but not to limit the present invention, and the present invention is described in detail with reference to the preferred embodiments. It should be understood by those skilled in the art that various modifications and equivalent substitutions may be made to the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention, and the scope of the claims of the present invention should be covered thereby.

Claims

1. The container is characterized by comprising a sample placing area, a suction head placing area, a reagent tube placing area and a PCR test tube placing area, wherein the sample placing area is arranged in the middle of the container, the suction head placing area is positioned on the left side of the sample placing area, the PCR test tube placing area is positioned on the right side of the sample placing area, and the reagent tube placing area is positioned on the front side of the sample placing area; the sample placing area is provided with one or a plurality of grooves arranged in parallel at intervals, the bottoms of the grooves are hollow, one sides of the grooves are communicated with the container to form one or more accommodating spaces for placing samples, the suction head placing area, the PCR test tube placing area and the reagent tube placing area are respectively provided with one or more first hole sites in the upward direction, and the one or more first hole sites in each area are respectively used for placing a suction head device, a PCR test tube and a reagent tube.

2. The container for loading samples and consumables in an integrated manner according to claim 1, wherein the tip placement area, the PCR tube placement area and the reagent tube placement area are respectively provided with a fixing base, the fixing base is detachably connected with the container, the fixing base is provided with a second hole corresponding to the first hole, and the second hole is aligned with the corresponding first hole for fixing the tip device, the PCR tube and the reagent tube.

3. The integrated sample and consumable loading container of claim 2, wherein the fixing base of the tip placement region is provided with one or more second hole sites having different inner diameters.

4. The integrated sample and consumable loading container of claim 2, wherein the second hole site has an inner diameter larger than the outer diameter of the tip device, PCR tube and reagent tube.

5. The integrated sample and consumable loading container according to claim 1, wherein the first holes of the tip placement region are arranged in a column, the first holes of the PCR tube placement region are arranged in a row, and the first holes of the reagent tube placement region are arranged in a column.

6. The container for integrally loading the samples and the consumables as claimed in claim 1, wherein the left and right sides of each accommodating space are provided with guide rails, and the samples slide into the accommodating spaces from the outside to the inside along the guide rails from the side where the container body is communicated with the groove.

7. The container for loading the samples and the consumables as claimed in claim 6, wherein a spring plate is provided above the guide rail and fixed on the accommodating space by a screw.

8. The integrated sample and consumable loading container of claim 6, wherein the sample is a slide or a cell culture dish.

9. The integrated sample and consumable loading container of claim 1, wherein the container body is provided with different markers in the tip placement area, the PCR tube placement area and the reagent tube placement area.

10. The integrated sample and consumable loading container of claim 1, wherein the container body is made of metal.