CN217709406U - Liquid-transfering needle tubing array, safety cover, liquid-transfering component and liquid-transfering system - Google Patents

Abstract

The application discloses pipette tip array, safety cover, move liquid subassembly and move liquid system, this pipette tip array includes: the array body is fixedly provided with pipette needle tubes, a push rod and a piston arranged at the end part of the push rod are arranged in a tube cavity of each pipette needle tube, and the tube cavity is also provided with a needle head communicated with the tube cavity. A protective cover, comprising: the protective cover comprises a protective cover body and protective holes, wherein the protective holes are arranged in an array of at least one line and at least one column, and the protective holes are matched with the pipette needle tube array. A pipetting assembly comprising: the pipette needle array is matched with the protective cover. A pipetting system comprising: the liquid transfer component, the sampling tube rack and the orifice plate are arranged in a manner that the liquid transfer needle tube array is used for taking and placing liquid in the sampling tube or the orifice plate. At least partial push rod top interconnect that sets up of adjacent setting of this application pipetting needle tubing array sets up, can realize batchization's liquid-transfering operation. In the application, the pipetting needle tube array does not need to be subjected to pitch change, so that a large amount of time in the sample nucleic acid extraction process is saved, and a large amount of cost is saved.

Description

Liquid-transfering needle tubing array, safety cover, liquid-transfering component and liquid-transfering system

Technical Field

The application belongs to the technical field of PCR detects, concretely relates to move liquid needle tubing array, safety cover, move liquid subassembly and move liquid system.

Background

At present, the requirement for nucleic acid detection is large, and the requirement for time and accuracy are high, but the current PCR (Polymerase Chain Reaction) detection equipment cannot meet the above requirements, and the following problems specifically exist:

for example, in an uncapping procedure, a sampling tube with a screw cap is generally used for sampling nucleic acids at present. Before the sampling tube is transported to a laboratory for sample nucleic acid extraction, the screw cap needs to be unscrewed, and if manual uncovering is adopted, long time and heavy workload are needed. If an automatic instrument is adopted for completion, an automatic cover opener is additionally adopted for completion.

For example, in the cup separation process, the sample in the sampling tube needs to be added to the well plate of the nucleic acid extractor before entering the nucleic acid extractor for nucleic acid extraction. If manual sample adding is adopted, long sample adding time and heavy workload are needed; if an automatic instrument is adopted for completion, a distance-variable liquid transfer device is required for completion, and the price of the current distance-variable liquid transfer device is very high.

In the process of extracting nucleic acid from a sample, a pipette is generally used to sample from an open sampling tube and apply the sample to a well plate, and then the sample to be treated or waste liquid is transferred in the open well plate by using the pipette, or magnetic beads having nucleic acid adsorbed thereon are transferred by using a magnetic rod. While the open sampling tube and the open orifice plate themselves present a risk of contamination from each other. During the transfer, the distance between pipettes or between magnetic bars is only a few millimeters, and contamination is also likely to occur. These are all responsible for the test result being "false positive". If heating is performed in the links of cracking, elution and the like, the risk of pollution is further increased.

In the process of adding the sample to the PCR amplification pore plate after the sample nucleic acid is extracted, the central shaft distance of the PCR amplification pore plate is also very small and open, so that the conditions of pollution and false positive are easy to occur.

SUMMERY OF THE UTILITY MODEL

To overcome the above-mentioned shortcomings or drawbacks of the prior art, the present application provides a pipette tip array, a protective cover, a pipette assembly, and a pipette system.

In order to solve the technical problem, the application is realized by the following technical scheme:

the application provides a pipette needle tubing array, include: the array comprises an array body, wherein at least one row and at least one column of liquid-transfer needle tubes are fixedly arranged on the array body and arranged in an array manner, the row number of the liquid-transfer needle tubes is N, the column number of the liquid-transfer needle tubes is M, and both N and M are integers larger than 0; a push rod and a piston arranged at the end part of the push rod are arranged in a tube cavity of each liquid-transfering needle tube, the tube cavity is also provided with a needle head communicated with the tube cavity, wherein the tops of at least part of the push rods which are adjacently arranged are mutually connected; and a plurality of holes connected with a suction power system are formed in the connecting plate at the top of the push rod.

Optionally, the top parts of the push rods in the same row or column are mutually connected and arranged.

Optionally, in the above pipette tip array, the central axes of the pipette tips arranged adjacently are equally spaced.

Optionally, in the above pipette tip array, the distance between the central axes of the pipette tips adjacently arranged is 9mm.

Optionally, the pipette needle array described above, wherein a lower limiting structure and/or an upper limiting structure is further provided on the push rod or inside the lumen.

Optionally, in the above pipette needle array, the push rod is further provided with a reinforcing rib at least parallel or perpendicular to the cross section of the lumen.

Optionally, in the above pipette tip array, the lower limiting structure includes: the blocking piece is arranged on the push rod, and the diameter of the blocking piece is larger than the aperture of the tube cavity.

Optionally, the pipette needle tube array is further provided with a protrusion at a portion of the lower part of the lumen connected to the needle.

Optionally, the pipette needle tube array may further comprise a tip angle of the bevel of the needle head of 20 ° to 40 °.

The present application further provides a protective cover, including: the protective cover comprises a protective cover body and a protective hole, wherein the row and column arrangement and the central shaft interval of the protective hole are the same as those of the pipette needle tube, and the protective hole is matched with the pipette needle tube.

Optionally, the above-mentioned protecting cover, wherein the inner diameter of the protecting hole is in interference fit or over-fit with the outer diameter of the pipette tip protrusion.

Optionally, the shield cap wherein the depth of the shield hole is greater than the total height of the spike and the protrusion.

Optionally, the above protective cover, wherein the pipette needle array and the protective cover are both provided with a positioning structure.

The application also provides a move liquid subassembly, include: the pipette needle tube array and the protective cover, wherein, the pipette needle tube array with the protective cover cooperation is used.

The application also provides a pipetting system, including: pipette subassembly, sampling tube and rather than the sampling tube support that the cooperation was used, be provided with the sampling tube groove that the array of at least one line, at least one row was arranged on the sampling tube support, the line of pipette tube, arrange with the central shaft interval all with the line of sampling tube groove, arrange with the central shaft interval the same, the pipette tube array at least be used for to get liquid in the sampling tube.

Optionally, the pipetting system further includes: the central axis spacing of the hole grooves in the hole plate is the same as the central axis spacing of the pipette needle tubes, the number of lines of the hole grooves is A multiplied by N, the number of columns of the hole grooves is B multiplied by M, and N, M, A and B are integers greater than 0, and the pipette needle tube array is also used for taking and placing liquid in the hole plate.

Compared with the prior art, the method has the following technical effects:

this application liquid-transfering needle tube array moving as a whole, the push rod top interconnect setting of at least partial adjacent setting can realize batchization move liquid operation etc.. The tops of the push rods in the same row or column are connected with each other, so that the power requirement of each group of the push rods connected with each other on the suction power system is not too high and is relatively even.

In the application, a lower limiting structure and/or an upper limiting structure is/are further arranged inside the push rod or the tube cavity. When the push rod drives the piston to perform suction action, the upper limit structure limits the highest position of the piston stroke, so that the maximum amount of liquid sucked into the pipe cavity is limited, and the highest position which can be reached by the liquid level in the pipe cavity is always lower than the lowest position of the piston stroke; when the push rod pushes the piston to perform liquid discharging operation, the lower limiting structure limits the lowest position of the piston stroke, so that the lowest position of the piston stroke is always higher than the highest position which can be reached by the liquid level in the tube cavity.

In this application, the sharp angle of the bevel of the needle is 20 ° to 40 °. The oblique cutting surface angle of a general liquid transfer needle is only 10 degrees, when a sampling tube or an orifice plate is sealed, although the sharp end of the needle is favorable for puncturing the sealing layer of the sampling tube or the orifice plate, because the longitudinal height of the needle hole is overlarge, when the height of liquid in an orifice groove of the orifice plate is smaller, more liquid is remained and cannot be taken out, and the needle is easy to drip. In the application, the sharp angle of the inclined cutting surface of the needle head is set to be 20-40 degrees, so that the needle head is favorable for poking a sampling pipe and a pore plate; the liquid at the bottom of the pore plate is easy to be taken out, and the liquid at the needle head is not easy to drip.

In the application, a reinforcing rib which is parallel or vertical to the section of the pipe cavity is arranged between the lower limit structure and the piston.

In the application, the central shaft spacing of the hole grooves in the hole plate, the central shaft spacing of the sampling tube grooves and the central shaft spacing of the pipette tubes are the same; the row and column arrangement of the pipette needle tubes is the same as that of the sampling tube grooves; the row and column arrangement of the hole grooves in the hole plate is the same as or in a multiple relation with the row and column arrangement of the sampling tube grooves and the pipette tubes, so that the capping and cup dividing operation is not needed before the sample nucleic acid extraction is carried out, the distance change of the pipette tubes is also not needed, a large amount of time for the sample nucleic acid extraction process is saved, and a large amount of equipment cost and time cost are also saved.

Drawings

Other features, objects and advantages of the present application will become more apparent upon reading of the following detailed description of non-limiting embodiments thereof, made with reference to the accompanying drawings in which:

FIG. 1: a perspective view of an array of pipette tips according to an embodiment of the present application;

FIG. 2 is a schematic diagram: a cross-sectional view of an array of pipette tips according to an embodiment of the present application;

FIG. 3: the structure of a part of a pipetting needle tube array in one embodiment of the application is shown as I;

FIG. 4: a partial structure diagram of the pipette needle tube array in the embodiment of the application is shown as II;

FIG. 5 is a schematic view of: a perspective view of a pipetting assembly according to one embodiment of the application;

FIG. 6: a top view of a pipetting assembly according to an embodiment of the present application;

FIG. 7 is a schematic view of: a cross-sectional view of a pipetting assembly according to an embodiment of the application;

FIG. 8: a perspective view of a protective cover of an embodiment of the present application;

FIG. 9: an enlarged view of a portion of the tip of a needle according to one embodiment of the present application.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

As shown in fig. 1 to 4, in one embodiment of the present application, a pipette tip array 21 includes: the array comprises an array body 216, wherein at least one row and at least one column of pipette needles 216A arranged in an array are fixedly arranged on the array body 216, each pipette needle 216A is provided with a lumen 217, a push rod 211 and a piston 212 arranged at the end of the push rod 211 are arranged in each lumen 217, the lumen 217 is further provided with a needle 215 communicated with the lumen 217, and the tops of at least part of the push rods 211 arranged adjacently are mutually connected. Through the arrangement, the liquid transferring operation in batches can be realized. Specifically, the top of pushrod 211 in each lumen 217 can be fixedly connected to the top of pushrod 211 in the same column, row, or other adjacent arrangement. Means of securing the connection include, but are not limited to, co-injection molding into one piece, and the like. A plurality of holes 2112 connected with a suction power system are arranged on a connecting plate 2111 at the top of the push rod 211. In this embodiment, the number of rows of the pipette needle 216A on the array body 216 is N, the number of columns is M, and N and M are integers greater than 0.

The tops of the push rods 211 in the same row or column are connected with each other through the connecting plate 2111, so that the power requirement of each group of the push rods 211 connected with each other on the suction power system is not too high and is relatively even.

The distances D between the central axes of the pipette tips 216A arranged adjacently are equal, as shown in fig. 2, 3, 7, and 8. That is, in this embodiment, the distance D between the center axes of the pipette tips 216A is set to be the same as the distance between the center axes of the sampling tube wells in the sampling tube rack described below.

Further preferably, the distance D between the central axes of the pipette tips 216A arranged adjacently is 9mm.

It is further preferred that the array body 216 further be provided with a positioning mechanism that can facilitate gripping by a robotic arm or automated transport assembly. In this embodiment, the positioning mechanism is a flange 218 at the top of the array body 216.

And a lower limiting structure 213 is also arranged on the inner part of the push rod 211 or the tube cavity 217.

When the push rod 211 drives the piston 212 to perform a pumping action, the upper limit structure limits the highest position of the stroke of the piston 212, so as to limit the maximum amount of liquid pumped into the tube cavity, and the highest position which can be reached by the liquid level in the tube cavity 217 is always lower than the lowest position of the stroke of the piston 212.

When the push rod 211 pushes the piston 212 to perform a liquid discharging operation, the lower limit structure 213 limits the lowest position of the stroke of the piston 212, so that the lowest position of the stroke of the piston 212 is always higher than the highest position that the liquid level in the tube cavity 217 can reach.

Certainly, in specific implementation, the control system can also control the maximum stroke of the push rod 211 up and down, so as to achieve the technical effects of upper and lower limiting.

As shown in fig. 1, fig. 2, and fig. 4 to fig. 7, the lower limit structure 213 includes: the blocking piece is arranged on the push rod 211, and the diameter of the blocking piece is larger than the aperture of the tube cavity 217. When the push rod 211 descends until the blocking piece is contacted with the upper edge of the tube cavity 217, the push rod 211 cannot descend continuously, so that the push rod 211 and the piston 212 reach the lowest position of the stroke; and the maximum upward stroke of the push rod 211 and the piston 212 is controlled by a control system. In this way, when the pipette tip array 21 performs a liquid taking or discharging operation, the piston 212 is always at a distance from the liquid surface, and the piston 212 is always at a distance from the highest position that the liquid surface can reach, so that the piston 212 and the plunger 211 are prevented from being contaminated by the liquid, and further, the piston 212 moves to contaminate the upper portion of the inner wall of the lumen 217, thereby spreading the contamination.

In order to facilitate the pushing of the piston 212, a lubricating substance, such as silicone oil, is usually coated on the inner wall of the lumen 217, and the upper and lower limiting structures 213 are further configured to effectively prevent the lubricating substance from being lost after contacting with the solution, which may increase the push-pull resistance of the push rod 211.

At least a first reinforcing rib 214 parallel to the section of the lumen and a second reinforcing rib 219 perpendicular to the section of the lumen are further provided on the push rod 211, and preferably, the first reinforcing rib 214 is further perpendicular to the second reinforcing rib 219.

Further, in this embodiment, the lower portion of the lumen 217 connected to the needle 215 is further provided with a protrusion. Wherein the outer diameter of the protrusion is smaller than the inner diameter of the cavity at the upper part of the tube cap of the sampling tube described below, so that when the pipette needle array 21 descends to the sampling tube, the protrusion extends into the cavity at the upper part of the tube cap of the sampling tube. Further, when the pipette needle array 21 descends to the protective cap 22, the needle 215 and the protrusion protrude into the protective hole 221, and the outer diameter of the protrusion and the inner diameter of the protective hole 221 are in interference fit or over-fit.

In this embodiment, the sharp angle of the bevel of the needle 215 is 20 ° to 40 °, which can ensure that the sharp end can easily dig into the sampling tube and the orifice plate, and can make the solution at the bottom of the orifice plate easily be taken out. As shown in fig. 9, the bevel angle of the needle 215 is 40 °, which is merely illustrative and not intended to limit the scope of the present application.

As shown in fig. 5 to 8, the present embodiment also proposes a protective cover 22 including: the protective cover 22 comprises a protective cover 22 body and protective holes 221, wherein the protective holes 221 are arranged in an array of at least one row and at least one column, and the protective holes 221 are matched with the pipette needle tube array 21.

The central axes of the adjacent protection holes 221 are spaced at equal intervals. That is, the row, column or array of the protection holes 221 and the central axis pitch arrangement thereof are identical to and correspond to the row, column or array of the pipette tips 216A and the central axis pitch arrangement thereof one by one, so that each of the tips 215 can be inserted into its corresponding protection hole 221.

Further preferably, the center-to-center distance between the adjacent protection holes 221 is 9mm.

In this embodiment, the protection hole 221 is formed to a depth greater than the total height of the needle 215 and the protrusion, thereby providing a mounting and protecting effect for the needle 215.

Further preferably, the inner diameter of the protection hole 221 and the outer diameter of the protrusion are in interference fit or over-fit.

The body of the protective cover 22 is further provided with a positioning structure, and the positioning structure can facilitate the mechanical arm or the automatic conveying assembly to grab or facilitate the mechanical arm or the automatic conveying assembly to be fixed in a working state. In this embodiment, the positioning mechanism is a flange 222 at the bottom of the body of the protective cover 22.

As shown in fig. 5 to 7, the present embodiment also proposes a pipetting assembly 20, including: the pipette needle array 21 and the protective cap 22, wherein the pipette needle array 21 is used in cooperation with the protective cap 22. The technical solutions of the pipette needle array 21 and the protection cap 22 are described above, and are not described herein again.

This embodiment also proposes a pipetting system, comprising: the sampling tube rack is provided with at least one row of sampling tube grooves arranged in an array manner, and at least one column of sampling tube grooves arranged in an array manner. The row, column and central axis spacing of the pipette tips 216A are the same as the row, column and central axis spacing of the sample cell wells, and the array of pipette tips 21 is used to extract fluid from the sample cells and to extract fluid from the well plates described below.

The pipetting system further comprises: the orifice plate, the central axis interval in orifice plate well hole groove with pipette tube 216A the center pin interval in sampling tube groove is the same, the sampling tube groove the line number of pipette tube 216A is N, and the line number is M, the line number in orifice plate hole groove is A N, and the line number is B M, and N, M, A, B are the integer that is greater than 0. For example: when the sampling tube slot is 48 holes (M =6, n = 8); the well plate has 96 wells (M =12, n = 8), the array of wells in the well plate is 2 times the array of sampling tube wells, the array of syringes samples from the sampling tubes and loads the samples into 2 sets of wells in the well plate, respectively, and the 2 sets of wells can be used for 2-tube detection of the same sample.

The pore plate can be empty or can be used for storing reagents in a sealing mode. When the pore plate is empty, the pore plate can be used as a pore plate for nucleic acid extraction processes such as cracking, magnetic bead capture, washing, elution and the like; it can also be used as a waste liquid well plate for receiving waste liquid from the nucleic acid extraction process. When the well plate is used for sealing reagents, the well plate can be used for sealing reagents required by a sample nucleic acid extraction (such as lysis, magnetic bead capture, washing and elution) process, such as: lysis solution, magnetic bead solution, washing solution, eluent and the like to form a nucleic acid extraction reagent pore plate; can also be used for sealing reagents required by PCR amplification and real-time fluorescence detection, such as: PCR amplification reagent and freeze-drying reagent to form the PCR amplification reagent pore plate.

The orifice plate, the pipette tube array 21, the sampling tubes and the sampling tube rack can be used in an automatic PCR detector in a matching way.

Specifically, sampling pipe prepackage virus conserver liquid, after the sampling is accomplished, put into the sampling pipe with the sampling swab rupture, rotatory tube cap seals the chamber that holds of sampling pipe. When the sampling tubes are placed in the sampling tube rack, the array arrangement mode and the central shaft spacing of the sampling tubes are completely the same as the array of the pipette needle tube array 21.

The sampling tube rack filled with the sampling tubes is placed in an automatic PCR (polymerase chain reaction) pipetting system and fixed, a mechanical arm in the PCR pipetting system can conveniently grab 21 arrays of pipetting needle tubes, each needle tube can conveniently align to the sampling tube and puncture a sealing gasket on a tube cover, and liquid is taken out in a containing cavity. When the pipette needle tube array 21 is withdrawn, the self-sealing performance of the sealing gasket enables each sampling tube to be automatically sealed, so that the single sampling tube is always in a sealing state and cannot be polluted by each other. Because the surface area of the needle is far smaller than that of the common pipetting tube, the solution inevitably remained on the outer wall of the needle in the pipetting process is necessarily far smaller than that remained on the outer wall of the pipetting tube; when the pipette tip array 21 is withdrawn from the elastomeric seal, most of the solution is trapped inside the elastomeric seal due to the extrusion of the elastomeric seal against the tip, further reducing the amount of solution on the outer wall of the tip. Compared with the outer wall space of about 4mm of a common pipetting pipe, the distance between adjacent needles is almost equal to 9mm, and the possibility of mutual pollution is further reduced.

And then, when the sample nucleic acid is extracted, the mechanical arm drives the pipetting needle tube array 21 array to align to the pore plate, so that liquid is spitted and taken out in the pore groove, and the steps of cracking, magnetic bead adsorption, washing, elution and the like are completed.

Because the central axial distance of the sampling tube grooves, the central axial distance of the hole plate hole grooves and the central axial distance of the pipette tubes 216A of the sampling tube frame are the same, and the central axial distances are the same or are in a multiple relation in the line and row arrangement, the capping and cup separation operation is not required before the sample nucleic acid extraction is carried out, the distance change of the pipette tube array is also not required, a large amount of sample nucleic acid extraction process time is saved, and a large amount of equipment cost and time cost are also saved.

The above embodiments are merely to illustrate the technical solutions of the present application and are not limitative, and the present application is described in detail with reference to preferred embodiments. It should be understood by those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof without departing from the true spirit and scope of the claims which follow.

Claims (14)

1. An array of pipette tips, comprising: the array comprises an array body, wherein at least one row and at least one column of pipette needle tubes are fixedly arranged on the array body, the number of rows of pipette needle tubes is N, the number of columns of pipette needle tubes is M, and both N and M are integers greater than 0; each pipette needle tube is internally provided with a push rod and a piston arranged at the end part of the push rod, the tube cavity is also provided with a needle head communicated with the tube cavity, and at least part of the tops of the push rods which are adjacently arranged are mutually connected.

2. The pipette tip array of claim 1, wherein the central axes of adjacently disposed pipette tips are equally spaced.

3. The pipette tip array of claim 2, wherein the center axes of adjacently disposed pipette tips are 9mm apart.

4. The pipette tip array of claim 1, wherein a lower and/or upper limit structure is further provided on the pushrod or inside the lumen.

5. The pipette needle array of claim 4, wherein the lower stop structure comprises: the blocking piece is arranged on the push rod, and the diameter of the blocking piece is larger than the aperture of the tube cavity.

6. The pipette needle array according to claim 1, wherein the portion of the lower part of the lumen connected to the needle is further provided with a projection.

7. Pipette tip array according to claim 1, characterized in that the bevel angle of the needles is 20 ° to 40 °.

8. A protective cover, comprising: a protective cover body and protective holes, wherein the row, column and central axis spacing of the protective holes are the same as the row, column and central axis spacing of the pipette needles according to any one of claims 1 to 7, and the protective holes are matched with the pipette needles.

9. The protective shield of claim 8 wherein the inner diameter of the protective aperture is an interference or over-fit with the outer diameter of the pipette tip protrusion.

10. The protective cover of claim 9, wherein the depth of the protective hole is greater than the total height of the spike and the protrusion.

11. A pipetting assembly comprising: the pipette tip array of any of claims 1 to 7 and the shield of any of claims 8 to 10, wherein the pipette tip array is used in conjunction with the shield.

12. A pipetting assembly as recited in claim 11 wherein the array of pipette tips and the shield are provided with locating features.

13. A pipetting system, comprising: the pipette assembly, sampling tube and sampling tube rack used in combination with the same as in claim 11 or 12, wherein the sampling tube rack is provided with at least one row and at least one column of sampling tube slots, the row, column and central axis spacing of the pipette tubes are the same as the row, column and central axis spacing of the sampling tube slots, and the array of pipette tubes is at least used for taking liquid into the sampling tube.

14. A pipetting system as recited in claim 13 further comprising: the central axis spacing of the hole grooves in the hole plate is the same as the central axis spacing of the pipette needle tubes, the number of lines of the hole grooves is A multiplied by N, the number of columns of the hole grooves is B multiplied by M, and N, M, A and B are integers greater than 0, and the pipette needle tube array is also used for taking and placing liquid in the hole plate.

Images