CN214270834U

CN214270834U - Eight-connecting-tube capping device for nucleic acid amplification

Info

Publication number: CN214270834U
Application number: CN202023059011.7U
Authority: CN
Inventors: 刘培海; 麻丽丹; 王殿夫; 王玉兰; 韩羽宣; 王浩; 于媛; 孟祥英; 梁娟娟; 杨丽丽
Original assignee: Dandong Customs Comprehensive Technical Service Center; Rizhao International Travel Health Care Center Rizhao Customs Port Outpatient Department; Rizhao Customs Integrated Technical Service Center; Eastern Liaoning University
Current assignee: Dandong Customs Comprehensive Technical Service Center; Rizhao International Travel Health Care Center Rizhao Customs Port Outpatient Department; Rizhao Customs Integrated Technical Service Center; Eastern Liaoning University
Priority date: 2020-12-18
Filing date: 2020-12-18
Publication date: 2021-09-24
Anticipated expiration: 2030-12-18

Abstract

The utility model relates to the technical field of biological instruments correlation, in particular to an eight-connecting-tube sealing cover device for nucleic acid amplification, which comprises a tray seat and a positioning seat arranged above the tray seat, wherein the tray seat is of a rectangular seat structure, the positioning seat is of a long rod structure, a rod body is positioned and fixed on the tray seat through a supporting rod, the positioning seats are symmetrically provided with a group, guide rails are arranged on the lower surfaces of the positioning seats at equal intervals, and the guide rails on the lower surfaces of the two positioning seats are correspondingly arranged; through having seted up the mounting hole on the tray seat to set up by placing the seat among the vestibule of mounting hole, the bearing structure that supporting component makes up and constitutes, and set up the guide rail at the lower surface of positioning seat, and through movable mounting have the setting element on the guide rail, thereby carry out the position to the bottom tube of eight even pipes, upper cover through the effect of setting element and prescribe a limit to, thereby guarantee its stability between the two, avoid in the heating process, pressure increase and lead to the emergence of upper cover obscission in its bottom tube.

Description

Eight-connecting-tube capping device for nucleic acid amplification

Technical Field

The utility model relates to a biological instrument correlation technique field specifically is a nucleic acid amplification is with eight connecting tube closing cap devices.

Background

With the application and popularization of molecular biology technology, nucleic acid amplification technology plays an increasingly important role in the detection and identification of bacteria, viruses and fungi. Fluorescent quantitative PCR methods are widely favored by research and detection laboratories for their simplicity, rapidity, robustness, and quantifiability. The fluorescent quantitative PCR reaction comprises the processes of sample treatment, nucleic acid extraction, nucleic acid amplification and the like. The nucleic acid amplification usually uses eight connecting tubes, the prepared reaction system and nucleic acid are added into the eight connecting tubes, and the eight connecting tubes are sealed and then detected by a fluorescent quantitative PCR instrument. The capping device adopts manual work to ensure that each hole is completely sealed, otherwise, liquid vapor overflows along with the rise of temperature in the nucleic acid process, so that cross contamination is caused, and even nucleic acid amplification equipment is polluted, so that false positive is caused. Manual capping is complicated, and especially when the amount of samples is large, the working efficiency is influenced; manual capping may also cause individual wells to fail to seal tightly, with potential biological safety risks; therefore, the utility model provides an eight-connecting-tube capping device for nucleic acid amplification, which is used for solving the problems.

Disclosure of Invention

An object of the utility model is to provide a nucleic acid amplification is with eight connecting tubes closing cap devices to solve the problem that proposes among the above-mentioned background art.

In order to achieve the above object, the utility model provides a following technical scheme: an eight-connecting-tube sealing cover device for nucleic acid amplification comprises a tray seat and positioning seats arranged above the tray seat, wherein the tray seat is of a rectangular seat structure, the positioning seats are of a long rod structure, rod bodies of the positioning seats are positioned and fixed on the tray seat through supporting rods, the positioning seats are symmetrically arranged in a group, guide rails are arranged on the lower surfaces of the positioning seats at equal intervals, the guide rails on the lower surfaces of the two positioning seats are correspondingly arranged, mounting holes are formed in the upper surface of the tray seat, the mounting holes are arranged at equal intervals and are provided with twelve rows and eight rows, supporting structures are movably arranged in the mounting holes and are formed by combining a placing seat and supporting components, a placing groove is formed in the upper end of the placing seat, a bottom tube is placed in the placing groove, an upper cover is arranged above the bottom tube, and eight groups are arranged at equal intervals on the bottom tube and the upper cover, and adjacent bottom tubes are connected through a first connecting piece, adjacent upper covers are connected through a second connecting piece, eight groups of bottom tubes, the upper covers, the first connecting piece and the second connecting piece are combined to form eight connecting tubes, and positioning pieces are movably arranged on the guide rails on the lower surface of the positioning seat.

Preferably, the positioning member is a long rod, a guide rail groove is formed in the lower surface of the rod body, the guide rail groove is of an isosceles trapezoid-shaped groove opening structure, the size of the cross section of the groove opening is matched with that of the cross section of the guide rail, and the positioning member is movably mounted on the guide rail through the guide rail groove.

Preferably, the supporting assembly is composed of a first telescopic pipe, a second telescopic pipe, a supporting spring, a first connecting plate and a second connecting plate, the second telescopic pipe is movably arranged in the inner cavity of the first telescopic pipe, the supporting spring is sleeved on the first telescopic pipe and the second telescopic pipe, and the upper surface of the second connecting plate is connected with the bottom surface of the placing seat.

Preferably, after the supporting spring on the supporting component is used for positioning and fixing the eight-connected pipe by using the positioning piece, the spring body of the supporting spring is in a compressed state.

Preferably, the size of the placing groove on the placing seat is matched with the size of the lower end part of the bottom tube.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the tray seat is provided with the mounting hole, the hole cavity of the mounting hole is provided with the supporting structure formed by combining the placing seat and the supporting assembly, the lower surface of the positioning seat is provided with the guide rail, and the positioning piece is movably arranged on the guide rail, so that the bottom pipe and the upper cover of the eight-connected pipe are limited in position under the action of the positioning piece, the stability between the bottom pipe and the upper cover is ensured, and the phenomenon that the upper cover falls off due to the increase of pressure in the bottom pipe in the heating process is avoided;

2. and through set up the standing groove identical with the size at bottom tube lower extreme position on placing the seat to effectively improve the stability that the bottom tube was put on placing the seat.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view of the structure at A in FIG. 1;

FIG. 3 is a cross-sectional view of the tray seat of the present invention at the location of the mounting hole;

FIG. 4 is an enlarged view of the structure at B in FIG. 3;

FIG. 5 is an enlarged view of the structure at C in FIG. 3;

FIG. 6 is a cross-sectional view of a support structure;

FIG. 7 is a schematic view of an eight-tube connection;

fig. 8 is an enlarged view of the structure at D in fig. 7.

In the figure: the tray seat comprises a tray seat 1, a positioning seat 2, a supporting rod 3, a guide rail 4, a mounting hole 5, a supporting structure 6, a placing seat 7, a supporting assembly 8, a first telescopic pipe 9, a second telescopic pipe 10, a supporting spring 11, a first connecting plate 12, a second connecting plate 13, a placing groove 14, an eight-connecting pipe 15, a bottom pipe 16, an upper cover 17, a first connecting piece 18, a second connecting piece 19, a positioning piece 20 and a guide rail groove 21.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-8, the present invention provides a technical solution: an eight-connecting-tube sealing cover device for nucleic acid amplification comprises a tray seat 1 and a positioning seat 2 arranged above the tray seat 1, wherein the tray seat 1 is of a rectangular seat structure, the positioning seat 2 is of a long rod structure, a rod body of the positioning seat 2 is positioned and fixed on the tray seat 1 through a supporting rod 3, the positioning seats 2 are symmetrically provided with one group, guide rails 4 are arranged on the lower surfaces of the positioning seats 2 at equal intervals, the guide rails 4 on the lower surfaces of the two positioning seats 2 are correspondingly arranged, the upper surface of the tray seat 1 is provided with a plurality of mounting holes 5, the mounting holes 5 are arranged at equal intervals and are provided with twelve rows and eight columns, a supporting structure 6 is movably arranged in each mounting hole 5, each supporting structure 6 is formed by combining a placing seat 7 and a supporting component 8, a placing groove 14 is arranged at the upper end of the placing seat 7, a bottom tube 16 is placed in the placing groove 14, and an upper cover 17 is arranged above the bottom tube 16, eight groups of bottom tubes 16 and upper covers 17 are arranged at equal intervals, and the adjacent bottom tubes 16 are connected through first connecting pieces 18, and the adjacent upper covers 17 are connected by a second connecting piece 19, eight groups of bottom tubes 16, the upper covers 17, a first connecting piece 18 and the second connecting piece 19 are combined to form eight connecting tubes 15, a positioning piece 20 is movably arranged on the guide rail 4 on the lower surface of the positioning seat 2, by arranging the mounting hole 5 on the tray seat 1 and arranging the supporting structure 6 formed by combining the placing seat 7 and the supporting component 8 in the hole cavity of the mounting hole 5, a guide rail 4 is arranged on the lower surface of the positioning seat 2, a positioning piece 20 is movably arranged on the guide rail 4, so that the bottom tube 16 and the upper cover 17 of the eight-connected tube 15 are limited in position by the positioning part 20, thereby ensuring the stability between the two and avoiding the phenomenon that the upper cover 17 falls off due to the pressure increase in the bottom tube 16 in the heating process.

Furthermore, the positioning element 20 is a long rod, the lower surface of the rod body is provided with a guide rail groove 21, the guide rail groove 21 is of a notch structure with an isosceles trapezoid cross section, the size of the cross section of the notch is matched with that of the cross section of the guide rail 4, and the positioning element 20 is movably arranged on the guide rail 4 through the guide rail groove 21;

furthermore, the supporting component 8 is composed of a first extension tube 9, a second extension tube 10, a supporting spring 11, a first connecting plate 12 and a second connecting plate 13, the second extension tube 10 is movably arranged in the inner cavity of the first extension tube 9, the supporting spring 11 is sleeved on the first extension tube 9 and the second extension tube 10, and the upper surface of the second connecting plate 13 is connected with the bottom surface of the placing seat 7;

further, after the supporting spring 11 on the supporting component 8 is used for positioning and fixing the eight-connecting pipe 15 by using the positioning piece 20, the spring body is in a compressed state;

furthermore, the size of the placing groove 14 on the placing seat 7 is matched with the size of the lower end part of the bottom tube 16, and the placing groove 14 matched with the size of the lower end part of the bottom tube 16 is formed in the placing seat 7, so that the placing stability of the bottom tube 16 on the placing seat 7 is effectively improved;

the working principle is as follows: in actual use, an operator adds a sample solution of nucleic acid to be amplified into the bottom tube 16 on the eight-connected tube 15, after the sample solution is completely added into the bottom tube 16 on the entire eight-connected tube 15, the operator covers the bottom tube 16 through the upper cover 17, inserts the bottom tube 16 on the eight-connected tube 15 into the placing groove 14 on the placing seat 7 in the mounting hole 5, then movably mounts the positioning member 20 on the guide rail 4 on the positioning seat 2 on one side through the guide rail groove 21 on the positioning member, presses the eight-connected tube 15 downwards, then inserts the positioning member 20 onto the guide rail 4 on the positioning seat 2 on the other side, thereby position-limiting the bottom tube 16 and the upper cover 17 of the eight-connected tube 15 through the action of the positioning member 20, ensuring the stability between the two, and avoiding the falling-off of the upper cover 17 caused by the increase of pressure in the bottom tube 16 during heating, thereby causing contamination of the sample solution in the octant tubing 15.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a nucleic acid amplification is with eight connecting tube closing cap devices, includes tray seat (1) and positioning seat (2) of setting in tray seat (1) top, its characterized in that: the tray seat (1) is of a rectangular seat structure, the positioning seats (2) are of a long rod structure, rod bodies of the positioning seats are fixedly positioned on the tray seat (1) through supporting rods (3), the positioning seats (2) are symmetrically provided with a group of positioning seats, guide rails (4) are arranged on the lower surfaces of the positioning seats (2) at equal intervals, the guide rails (4) on the lower surfaces of the two positioning seats (2) are correspondingly arranged, the upper surface of the tray seat (1) is provided with a plurality of mounting holes (5), the mounting holes (5) are arranged at equal intervals and are provided with twelve rows and eight columns, supporting structures (6) are movably arranged in the mounting holes (5), each supporting structure (6) is formed by combining a placing seat (7) and a supporting component (8), the upper end of the placing seat (7) is provided with a placing groove (14), bottom tubes (16) are placed in the placing groove (14), and be provided with upper cover (17) above bottom tube (16), bottom tube (16) and upper cover (17) are equidistant totally and are provided with eight groups, and are connected through first connecting piece (18) between adjacent bottom tube (16), and link to each other for linking to each other through second connecting piece (19) between adjacent upper cover (17), and eight groups of bottom tube (16), upper cover (17) and first connecting piece (18), second connecting piece (19) combination constitution eight even pipe (15), the activity is provided with setting element (20) on guide rail (4) of positioning seat (2) lower surface.

2. The eight-tube sealing device for nucleic acid amplification according to claim 1, wherein: the positioning piece (20) is a long rod, and the lower surface of the rod body of the positioning piece is provided with a guide rail groove (21).

3. The eight-tube sealing device for nucleic acid amplification according to claim 2, wherein: the guide rail groove (21) is of a groove opening structure with an isosceles trapezoid cross section.

4. The eight-tube sealing device for nucleic acid amplification according to claim 1, wherein: the size of the cross section of the groove opening of the guide rail groove (21) is matched with that of the cross section of the guide rail (4).

5. The eight-tube sealing device for nucleic acid amplification according to claim 1, wherein: the positioning piece (20) is movably arranged on the guide rail (4) through a guide rail groove (21).

6. The eight-tube sealing device for nucleic acid amplification according to claim 1, wherein: the supporting assembly (8) is formed by combining a first telescopic pipe (9), a second telescopic pipe (10), a supporting spring (11), a first connecting plate (12) and a second connecting plate (13), the second telescopic pipe (10) is movably arranged in an inner cavity of the first telescopic pipe (9), the supporting spring (11) is sleeved on the first telescopic pipe (9) and the second telescopic pipe (10), and the upper surface of the second connecting plate (13) is connected with the bottom surface of the placing seat (7).

7. The eight-tube sealing device for nucleic acid amplification according to claim 1, wherein: after the supporting spring (11) on the supporting component (8) is used for positioning and fixing the eight-connecting pipe (15) by using the positioning piece (20), the spring body is in a compressed state.

8. The eight-tube sealing device for nucleic acid amplification according to claim 1, wherein: the size of the placing groove (14) on the placing seat (7) is matched with the lower end part of the bottom pipe (16).