CN219714956U - Cell slide glass dyeing device - Google Patents
Cell slide glass dyeing device Download PDFInfo
- Publication number
- CN219714956U CN219714956U CN202322169648.9U CN202322169648U CN219714956U CN 219714956 U CN219714956 U CN 219714956U CN 202322169648 U CN202322169648 U CN 202322169648U CN 219714956 U CN219714956 U CN 219714956U
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- China
- Prior art keywords
- slide
- dyeing
- staining
- space
- glass
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- 238000004043 dyeing Methods 0.000 title claims abstract description 50
- 239000011521 glass Substances 0.000 title claims abstract description 41
- 239000000741 silica gel Substances 0.000 claims abstract description 33
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 33
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000002699 waste material Substances 0.000 claims abstract description 17
- 238000010186 staining Methods 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 26
- 238000007789 sealing Methods 0.000 claims description 13
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 7
- 238000002474 experimental method Methods 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000012128 staining reagent Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011002 quantification Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a cell slide glass dyeing device, which belongs to the technical field of biological experimental instruments and equipment, and comprises a box body (1), wherein at least two dyeing spaces (2) are separated in the box body (1), a slide glass inlet (3) and a slide glass outlet (4) are arranged on the side surface of the dyeing space (2), a sample adding port (5) is arranged on the upper surface of the dyeing space (2), a slide glass carrier is arranged in the dyeing space (2), the slide glass carrier is a strip-shaped grating (6), and scales (10) are marked on the dyeing space (2); the glass slide inlet (3), the glass slide outlet (4) and the sample adding port (5) are respectively matched with a silica gel plug (7); a slide clamping groove (21) is formed in the dyeing space (2), and the slide clamping groove (21) is matched with the silica gel plug (7) to form a groove body with a closed side face. The utility model realizes the independent dyeing of a plurality of samples, quantitatively adds the dyeing reagent, improves the working efficiency, reduces the waste and reduces the risk of damaging the samples.
Description
Technical Field
The utility model relates to biological experimental equipment, in particular to a cell slide staining device.
Background
In biological experiments, there are a variety of detection experiments that require staining of cells and tissue sections, such as immunostaining, karyotyping, immunofluorescence, and the like. The size of the slides is currently standardized, with standard microscope slides having dimensions of about 75 mm x25 mm (3 inches x1 inch) and a thickness of about 1mm, but the storage containers for the dye solutions are not of fixed size, and various laboratories use plates, glass jars, beakers, etc., resulting in the following problems: (1) The container is not a standard container, cannot be well matched with a glass slide, cannot quantify the added dye liquor, and needs to be added with the dye liquor with larger volume when in use, so that the dye liquor is wasted, the cost is increased, and the dye liquor is a toxic and harmful chemical reagent and possibly causes environmental pollution; (2) Staining of only one sample at a time may lead to confusion of samples and risk of cross-contamination between samples if multiple samples are stained in one container at a time.
Disclosure of Invention
The utility model aims at: aiming at the problems, the cell slide staining device is provided, a device special for slide staining is designed, the quantitative dye liquor can be added accurately, and the waste is avoided; the device can dye a plurality of samples at the same time, and the dyeing of each sample is independent and not mixed up; the sealing design can place the dyeing box at the required temperature and humidity, so that the accuracy of the test is ensured.
The technical scheme of the utility model is as follows:
the utility model discloses a cell slide glass dyeing device, which comprises a box body, wherein at least two dyeing spaces are separated in the box body, a slide glass inlet and a slide glass outlet are arranged on the side surfaces of the dyeing spaces, a sample adding port is arranged on the upper surface of the dyeing space, a slide glass platform is arranged in the dyeing space, the slide glass platform is a strip-shaped grating, and scales are marked on the dyeing space; the glass slide inlet, the glass slide outlet and the sample adding port are respectively matched with silica gel plugs.
With the structure, the slide carrier with the strip-shaped grating is beneficial to taking out the slide; by marking scales on the dyeing space, the sample adding volume can be controlled, so that the sample can be fully impregnated with the reagent, and the waste of the reagent can be avoided; through setting up the silica gel plug, can seal around the device, guarantee that reagent can not leak, carry out the safety experiment.
Further, the two opposite sides of the staining space are respectively provided with a slide inlet and a slide outlet, or the slide inlet and the slide outlet are the same.
With the above structure, two ports can be provided or one port can be used according to the operation space, the use habit and the like.
Further, each dyeing space is provided with a liquid outlet, the liquid outlet is communicated with the waste liquid tank, and the waste liquid tank is communicated with all the dyeing spaces through the liquid outlet.
Above structure through setting up the leakage fluid dram, can pour out the waste liquid that the experiment produced, and unified processing can not pollute the environment.
Further, labels are attached to each of the sides of the staining space.
Above structure through setting up the label, can post experiment sample information, can not let the experimenter confuse.
Further, the box body is a transparent box body.
Above structure, transparent box body can clearly see the result of experiment, and convenient observation.
Further, a slide clamping groove is arranged in the dyeing space, the slide clamping groove corresponds to two groove surface openings of the slide inlet and the slide outlet, the heights of the other two groove surfaces are larger than the thickness of the slide, and a slide carrier is arranged at the bottom of the slide clamping groove; the silica gel plugs of the slide inlet and the slide outlet are respectively attached to the two groove surface openings and form a groove body with the side surfaces closed with the other two groove surfaces.
The structure is characterized in that the silica gel plug is skillfully combined with the slide clamping groove, the silica gel plug has the function of sealing the slide inlet, the slide outlet and the sample adding port, after the silica gel plug is sealed, the interior of the silica gel plug is attached to the slide clamping groove to form a groove body for adding dye liquor for dyeing, and a sample is placed in the groove body; after the silica gel plug is taken down, the waste liquid in the glass slide clamping groove flows out rapidly, and is discharged into the waste liquid groove through the liquid outlet, meanwhile, since the glass slide clamping groove is provided with only two groove surfaces, a sampling port is formed after the silica gel plug is taken down, and the glass slide can be taken out conveniently through the sampling port, if the groove bodies of the four groove surfaces are not good, the glass slide can be taken out. The glass slide clamping groove is arranged to reduce the bottom area of the dye liquor tank, the same amount of dye liquor is deeper, the glass slide is submerged for dyeing, and the dyeing can be performed by using less dye liquor in the same way, so that the using amount of the dye liquor is reduced; and the slide clamping groove is communicated with the external dyeing space, and when the dye liquor in the slide clamping groove is full, the slide clamping groove can be stored through the external dyeing space.
Further, the silica gel plug comprises an integral lifting part and a sealing part, wherein the sealing part is a wedge; the silica gel plug is connected with the box body through a rope.
In the structure, the sealing part is a wedge, and the sealing can be performed by plugging the tip part into the opening; the silica gel plug is connected with the box body through the rope, so that the silica gel plug is prevented from being lost after being pulled down.
Further, scales are marked on the slide clamping groove.
With the structure, the scales are marked on the slide clamping groove, so that the volume of the dye liquor in the slide clamping groove can be determined and controlled.
In summary, due to the adoption of the technical scheme, the beneficial effects of the utility model are as follows:
1. the utility model realizes the quantification of the added reagent, avoids the waste of the reagent, and simultaneously accurately controls the added quantity to meet the requirement.
2. The utility model is provided with a plurality of dyeing spaces at the same time, and the dyeing spaces are separated, so that the dyeing of a plurality of samples can be performed at the same time, the mutual influence and the risk of cross contamination are avoided; the labor and material resources are saved, and the working efficiency is improved.
3. The utility model provides a sealed dyeing space, which can ensure that dye liquor is not leaked and seal the interference of the outside; the sealing design can also place the dyeing box at the required temperature and humidity, so as to ensure the accuracy of the test.
4. The utility model is provided with the slide glass inlet and the slide glass outlet, so that the slide glass can be conveniently taken, and the damage to a sample is avoided.
5. The utility model skillfully combines the silica gel plug with the slide clamping groove, and the silica gel plug is used as a detachable groove surface, so that a dyeing groove body can be formed on the silica gel plug, and the silica gel plug can be taken down to facilitate liquid discharge and slide glass taking out.
Drawings
FIG. 1 is a schematic diagram of a cell slide staining apparatus according to the present utility model.
FIG. 2 is a left side view of the cell slide staining apparatus in the embodiment.
Fig. 3 is a schematic structural view of a silicone plug in an embodiment.
Fig. 4 is a schematic structural diagram of a dyeing space in the embodiment.
Fig. 5 is a schematic view of a slide card slot in an embodiment.
Reference numerals: 1-box body, 2-dyeing space, 21-slide clamping groove, 22-opening, 3-slide inlet, 4-slide outlet, 5-sample inlet, 6-strip grid, 7-silica gel plug, 71-lifting part, 72-sealing part, 73-rope, 8-waste liquid groove, 9-label and 10-scale.
Detailed Description
It is noted that relational terms such as "first" and "second", and the like, are used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Moreover, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one … …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The features and capabilities of the present utility model are described in further detail below in connection with examples.
As shown in fig. 1 and 2, the utility model discloses a cell slide staining device, which comprises a box body 1, wherein at least two staining spaces 2 are separated in the box body 1, a slide inlet 3 and a slide outlet 4 are arranged on the side surface of the staining space 2, a sample adding port 5 is arranged on the upper surface of the staining space 2, the sample adding port 5 is used for adding a staining reagent, a slide table is arranged in the staining space 2, and the slide table is used for bearing a slide; the dyeing space 2 is marked with a scale 10; the slide inlet 3, the slide outlet 4 and the sample inlet 5 are respectively matched with a silica gel plug 7 with matched size for sealing.
In the embodiment, each dyeing space 2 is provided with a liquid outlet, the liquid outlet is sealed by a silica gel plug 7, waste liquid generated in the experiment can be discharged, the liquid outlet is communicated with a waste liquid tank 8, and the waste liquid tank 8 is communicated with all the dyeing spaces 2 through the liquid outlet. Each staining space 2 is provided with a label 9 on the side surface, and the label 9 comprises experimental sample information, so that the confusion of experimental staff is avoided. The box body 1 is a transparent box body 1, and the experimental result can be clearly seen. The box body 1 is made of glass, the length of the box body is slightly larger than the size of the glass slide, and the height of the box body is the height of three glass slides stacked.
As shown in fig. 3, the silica gel plug 7 includes an integral lifting portion 71 and a sealing portion 72, and the sealing portion 72 is a wedge; the silica gel plug 7 is connected with the box body 1 through a rope 73.
In one embodiment, the two opposite sides of the staining space 2 are respectively provided with a slide inlet 3 and a slide outlet 4, the slide inlet 3 is a channel for placing a slide, the slide outlet 4 is a channel for taking out the slide, the size fits with the size of the slide, and the slide inlet 3 and the slide outlet 4 can be mutually exchanged. In another embodiment, the slide inlet 3 and slide outlet 4 are the same port, and are placed in and removed from the same port.
As shown in fig. 4 and 5, in one embodiment, a slide clamping groove 21 is arranged in the staining space 2, two groove surfaces of the slide clamping groove 21 corresponding to the slide inlet 3 and the slide outlet 4 are provided with openings 22, the heights of the other two groove surfaces are larger than the thickness of the slide, and the bottom of the slide clamping groove 21 is provided with a slide table; the silica gel plugs 7 of the slide inlet 3 and the slide outlet 4 are respectively attached to the openings 22 of the two groove surfaces and form a groove body with a closed side surface with the other two groove surfaces; the slide clamping groove 21 is marked with a scale 10. A slide carrier is arranged in the dyeing space 2, and the slide carrier is a strip-shaped grating 6. The width of the slide clamping groove 21 is matched with the slide, the left and right sides of the slide clamping groove are slightly larger than the width of the slide by 1mm, the height of the slide clamping groove 21 is twice the thickness of the slide, and if the slide clamping groove 21 is fully impregnated with the dye liquor, namely the height of the slide clamping groove 21 is three times the thickness of the slide.
Working principle: when dyeing is needed, the silica gel plugs of the slide inlet and the sample adding port are taken down, the slide is placed into the dyeing space from the slide inlet, and a plurality of slides can be placed into different dyeing spaces at the same time. A staining reagent is added from a sample adding port, the slide glass sample is soaked or contacted by the staining reagent, sample staining is carried out, and a silica gel plug is installed. After dyeing, taking down the silica gel plug at the outlet of the glass slide, taking out the dyed glass slide, simultaneously taking down the silica gel plug at the liquid outlet, and allowing the waste liquid after the experiment to enter the waste liquid tank from the liquid outlet.
The above examples merely illustrate specific embodiments of the utility model, which are described in more detail and are not to be construed as limiting the scope of the utility model. It should be noted that it is possible for a person skilled in the art to make several variants and modifications without departing from the technical idea of the utility model, which fall within the scope of protection of the utility model.
Claims (8)
1. The cell slide glass dyeing device is characterized by comprising a box body (1), wherein at least two dyeing spaces (2) are separated in the box body (1), a slide glass inlet (3) and a slide glass outlet (4) are arranged on the side face of the dyeing space (2), a sample adding port (5) is arranged on the upper surface of the dyeing space (2), a slide glass table is arranged in the dyeing space (2), the slide glass table is a strip-shaped grating (6), and scales (10) are marked on the dyeing space (2); the glass slide inlet (3), the glass slide outlet (4) and the sample adding port (5) are respectively matched with a silica gel plug (7).
2. A cell slide staining apparatus according to claim 1 wherein the staining space (2) is provided with a slide inlet (3) and a slide outlet (4) on two opposite sides, respectively, or the slide inlet (3) and the slide outlet (4) are the same port.
3. The device for staining a cell slide according to claim 1, wherein each staining space (2) is provided with a liquid drain port which communicates with the waste liquid tank (8), and the waste liquid tank (8) communicates with all staining spaces (2) through the liquid drain port.
4. The device for staining cell slides according to claim 1, characterized in that a label (9) is attached to each staining space (2) on the side.
5. The device for staining cell slides according to claim 1 or 3 or 4, wherein the cassette (1) is a transparent cassette.
6. The device for staining cell slide according to claim 1, wherein a slide clamping groove (21) is arranged in the staining space (2), two groove surfaces of the slide clamping groove (21) corresponding to the slide inlet (3) and the slide outlet (4) are provided with openings (22), the heights of the other two groove surfaces are larger than the thickness of the slide, and the bottom of the slide clamping groove (21) is provided with a slide carrier; the silica gel plugs (7) of the slide inlet (3) and the slide outlet (4) are respectively attached to the openings (22) of the two groove surfaces, and form a groove body with the side surfaces closed with the other two groove surfaces.
7. The device for staining a cell slide according to claim 6 wherein the silica gel plug (7) comprises an integral lifting part (71) and a sealing part (72), the sealing part (72) being a wedge; the silica gel plug (7) is connected with the box body (1) through a rope (73).
8. A cell slide staining apparatus according to claim 6 wherein the slide card slot (21) is marked with a scale (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322169648.9U CN219714956U (en) | 2023-08-14 | 2023-08-14 | Cell slide glass dyeing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322169648.9U CN219714956U (en) | 2023-08-14 | 2023-08-14 | Cell slide glass dyeing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219714956U true CN219714956U (en) | 2023-09-19 |
Family
ID=87976388
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322169648.9U Active CN219714956U (en) | 2023-08-14 | 2023-08-14 | Cell slide glass dyeing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219714956U (en) |
-
2023
- 2023-08-14 CN CN202322169648.9U patent/CN219714956U/en active Active
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