CN210886074U - PCR reaction consumable for automatic nucleic acid detection - Google Patents
PCR reaction consumable for automatic nucleic acid detection Download PDFInfo
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- CN210886074U CN210886074U CN201921709791.XU CN201921709791U CN210886074U CN 210886074 U CN210886074 U CN 210886074U CN 201921709791 U CN201921709791 U CN 201921709791U CN 210886074 U CN210886074 U CN 210886074U
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Abstract
The utility model discloses a PCR reaction consumable material for automatic nucleic acid detection, which relates to the field of biological experiment consumable materials, and has the technical scheme that the consumable material comprises a tube body, wherein the tube body is provided with a tube orifice and a tube cover for plugging the tube orifice, and the outer side wall of the tube body is provided with a pressing part for fixing the tube body when the tube cover is opened; the pipe cover comprises a cover plate and a cover body, and the cover plate is provided with a bulge part which is taken as a stress point when the pipe cover is pulled out of the pipe body; when the tube cover covers the tube body, a gap is formed between the pressing part and the protruding part; the cover body is provided with a cavity along the length direction of the cover body, and the cover plate is provided with a light-through part at the corresponding position of the cavity for light to enter the pipe body. Compare in current reaction consumptive material, the utility model provides a reaction consumptive material can realize under the cooperation of automatic nucleic acid detecting instrument that the automation of reaction consumptive material is uncapped, is closed the lid, and then can encapsulate reagent in the consumptive material, when satisfying the automation of nucleic acid detecting system, has also reduced the biological pollution risk among the PCR reaction process.
Description
Technical Field
The utility model relates to a biological assay consumptive material field, more specifically say, it relates to a PCR reaction consumptive material for automizing nucleic acid detection.
Background
PCR (polymerase chain reaction) is the most common method for detecting nucleic acid at present. The PCR reaction requires that a nucleic acid sample is mixed with different reagent components in a PCR reaction consumable, and the PCR reaction consumable bears reaction liquid and undergoes a plurality of temperature cycles to complete the amplification and detection of the nucleic acid. Meanwhile, the PCR method based on the detection sensitivity and the high efficiency of nucleic acid amplification can cause false positive detection results even if trace nucleic acid is polluted or aerosol is diffused, and even cause pollution to the whole laboratory.
At present, with the continuous update of technologies, related enterprises have produced automated instruments capable of realizing all the processes of nucleic acid detection, that is, all the steps of nucleic acid extraction, sample addition, pre-mixing, subpackaging, reaction, PCR method detection and the like are carried out in one instrument. In the use process of the existing automatic nucleic acid detection instrument, most of reaction consumables placed in the instrument cannot automatically open and close the cover.
A moving part that covers the tube cap on the consumptive material or pulls up the tube cap from the consumptive material can be arranged in the automatic nucleic acid detecting instrument for the whole flow of PCR reaction. Therefore, designing a PCR reaction consumable capable of automatically opening and closing a cover in cooperation with an automatic nucleic acid detection system avoids adding reagents and samples into the consumable manually by an operator, reduces the misoperation rate, avoids errors and pollution caused by manual operation, and is urgently required by related industries at present.
SUMMERY OF THE UTILITY MODEL
Not enough to prior art exists, the utility model aims to provide a PCR reaction consumptive material for automizing nucleic acid and detecting, it has the advantage that can realize uncapping, closing the lid operation of automation of PCR pipe under the cooperation of supporting instrument.
In order to achieve the above purpose, the utility model provides a following technical scheme:
a PCR reaction consumable for automatic nucleic acid detection comprises a tube body, a tube cover and a tube, wherein the tube body is provided with a tube opening;
the outer side wall of the pipe body is provided with a pressing part for fixing the pipe body when the pipe cover is opened;
the pipe cover comprises a cover plate and a cover body, and the cover plate is provided with a bulge part which is taken as a stress point when the pipe cover is pulled out of the pipe body;
when the tube cover covers the tube body, a gap is formed between the pressing part and the protruding part;
the cover body is provided with a cavity along the length direction of the cover body, and the part of the cover plate corresponding to the cavity is provided with a light-through part for light to enter the pipe body.
Through adopting above-mentioned technical scheme, be arranged in automatic nucleic acid detecting instrument with the reaction consumptive material, when needs uncap the reaction consumptive material, the movable member accessible in the instrument presses the splenium in order to fix the body, and another movable member can act on the bellying in order to pull up the tube cap simultaneously. When the pipe cover needs to be covered on the pipe body, the moving piece can press the cover plate to cover the pipe body with the pipe cover. In addition, the light-transmitting part can be used for light to enter, so that the accuracy of fluorescence collection in the PCR reaction process is ensured. Compare in current unable automatic nucleic acid detecting instrument of cooperation use in order to realize the automatic reaction consumptive material of uncapping, closing the lid, this scheme can realize the automatic of reaction consumptive material under the cooperation of automatic nucleic acid detecting instrument and open, close the lid, and then can encapsulate reagent in the consumptive material, when satisfying the automation of nucleic acid detecting system, also avoided the biological pollution risk among the PCR reaction process.
The connecting plate is inserted with a pipe body with a plurality of pipe orifices all in the same plane;
the pressing parts are arranged at the edges of the connecting plate, and a first yielding groove is formed between every two adjacent pressing parts.
Through adopting above-mentioned technical scheme, a plurality of body accessible connecting plate links together in order to satisfy the different demands to the reaction consumptive material in the reaction process. If the moving member in the automatic nucleic acid detecting apparatus cannot directly move into the gap between the pressing portion and the protruding portion, the moving member can be moved to the first avoiding groove first and then moved into the gap between the pressing portion and the protruding portion. The first groove of stepping down can supply the moving member to pass, and then is convenient for the moving member to press and press the splenium in order to fix the body.
The bottom surface of the pipe cover plate is provided with a plurality of pipe covers with axes vertical to the pipe cover plate;
the protruding parts are arranged on the edge of the pipe cover plate, and a second yielding groove is formed between every two adjacent protruding parts.
Through adopting above-mentioned technical scheme, a plurality of tube cap accessible tube cover board links together in order to satisfy the different demands to the reaction consumptive material among the reaction process. If the moving member in the automated nucleic acid detecting apparatus cannot directly move to the gap between the pressing portion and the protruding portion, the moving member may be moved to the second receding groove first, and then moved to the gap between the pressing portion and the protruding portion. The second abdicating groove can be used for the moving piece to pass through, so that the moving piece can act on the convex part conveniently to pull up the tube cover.
Furthermore, a limiting groove for embedding a fixing piece in the instrument to fix the pipe cover plate in the process of horizontally moving the pipe cover plate is formed in one side, which is far away from the pipe cover, of the pipe cover plate.
By adopting the technical scheme, after the pipe cover is pulled up, when the pipe cover is horizontally moved away from the position right above the pipe body by the moving part, the fixing part on the moving part can be inserted into the limiting groove to fix the pipe cover plate, so that the pipe cover plate is prevented from moving on the moving part or falling off from the moving part in the moving process, and subsequent cover closing and reaction processes are prevented from being influenced.
Further, the spacing groove is provided with at least two.
Through adopting above-mentioned technical scheme, fixed tube cover board that can be better avoids the tube cover board to take place to remove or drop from the moving member on the in-process that removes.
Further, the pipe orifice is arranged in a chamfer angle.
Through adopting above-mentioned technical scheme, can make the smooth lid of tube cap fit the body, reduce because of the sealed face bruise that the assembly caused.
Further, the sealing ring structure is used for increasing the matching sealing performance of the nozzle and the tube cover.
Through adopting above-mentioned technical scheme, guarantee to close the leakproofness in the consumptive material behind the lid, avoid taking place conditions such as nucleic acid pollution or aerosol diffusion in the reaction sequence.
Further, the periphery lateral wall of lid is provided with protruding circle, the mouth of pipe is provided with and is used for the sealing washer that is sunken formula with protruding circle inlay card complex.
Through adopting above-mentioned technical scheme, protruding circle and sealing washer inlay card cooperation guarantee to close the leakproofness in the consumptive material behind the lid, avoid taking place conditions such as nucleic acid pollution or aerosol diffusion in reaction process.
Further, the light-transmitting part is a light-transmitting part made of a polyester material with light-transmitting property.
By adopting the technical scheme, the light transmission part can be used for light to enter, and the accuracy of fluorescence acquisition in the PCR reaction process is ensured.
To sum up, the utility model discloses following beneficial effect has:
1. the pressing part arranged on the outer side wall of the pipe body and the protruding part arranged on the cover plate can be matched with moving parts in an automatic nucleic acid detection instrument for use, so that automatic cover opening and closing of reaction consumables are realized, further, reagents can be packaged in the consumables, and the biological pollution risk in the PCR reaction process is avoided while the automation of a nucleic acid detection system is met;
2. through set up the sealing washer of sunken formula and set up protruding circle at the lid at the mouth of pipe, at the tube cap lid with in the body time, the sealing washer can inlay card cooperation with protruding circle, has guaranteed the leakproofness in the reaction consumptive material, avoids taking place conditions such as nucleic acid pollution or aerosol diffusion in reaction process.
Drawings
FIG. 1 is a schematic diagram showing the overall structure of a consumable for PCR reaction used for automated nucleic acid detection in example 1;
FIG. 2 is an exploded view of the construction of a consumable for PCR reaction for automated nucleic acid detection in example 1;
FIG. 3 is a schematic view showing the structure of a cap for a tube in embodiment 1;
FIG. 4 is a schematic diagram showing the overall structure of a consumable for PCR reaction used in automated nucleic acid detection in example 2;
FIG. 5 is an enlarged view of portion A of FIG. 4;
FIG. 6 is a schematic diagram of the overall structure of the consumable PCR material for automated nucleic acid detection in example 3.
In the figure: 1. a tube cover; 11. a cover plate; 111. a through hole; 12. a cover body; 121. a protruding ring; 13. a boss portion; 14. a gap; 2. a pipe body; 21. a pipe body is arranged; 211. a seal ring; 22. a lower pipe body; 221. a cavity; 23. a pressing part; 24. a pipe orifice; 4. a light-transmitting portion; 5. a tube cover plate; 51. a second abdicating groove; 52. a limiting groove; 6. a connecting plate; 61. a first abdicating groove; 7. a special-shaped pipe.
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
Example 1:
a PCR consumable for automatic nucleic acid detection, referring to FIGS. 1, 2 and 3, comprises a tube body 2 and a cylindrical tube cover 1. The tube body 2 comprises a cylindrical upper tube body 21 and a conical lower tube body 22, and a tube opening 24 is formed at one end of the upper tube body 21 far away from the lower tube body 22. The tube cover 1 includes a cover plate 11 and a cover body 12 disposed on the cover plate 11, and a cavity 221 is formed in the cover body 12 along an axial direction of the cover body 12.
Referring to fig. 1 and 2, the outer circumferential side wall of the upper tube 21 is provided with two strip-shaped pressing portions 23 along the radial direction, and the two pressing portions 23 are distributed at equal intervals along the circumferential direction of the upper tube 21. Similarly, the outer peripheral side wall of the cover plate 11 is provided with two elongated protrusions 13 along the radial direction of the cover plate 11, and the two protrusions 13 are equally spaced along the circumferential direction of the cover plate 11. When the tube cover 1 is covered on the cover body 12, the protruding portions 13 correspond to the pressing portions 23 one by one, and a gap 14 is formed between the protruding portions 13 and the pressing portions 23.
Referring to fig. 1 and 2, a light-transmitting portion 4 made of a polyester material having a light-transmitting property is disposed on the cover plate 11 facing the cavity 221, so that light can enter the light-transmitting portion, thereby ensuring the accuracy of fluorescence collection during the PCR reaction.
Referring to fig. 2, the pipe opening 24 is chamfered to ensure that the pipe cap 1 is smoothly inserted into the pipe body 2, thereby reducing the damage caused by collision of the sealing surface due to assembly.
The working principle is as follows: when the reaction consumables are used for the automatic nucleic acid detection instrument and need uncap, the moving member accessible first groove of stepping down and the second groove of stepping down in the instrument remove to clearance 14, and the moving member is through pressing the splenium 23 with fixed body 2, and simultaneously, the moving member can regard bellying 13 as the stress point and then pull up tube cap 1. When the pipe cover 1 needs to be covered on the pipe body 2, the moving piece can move to the gap 14 through the first abdicating groove and the second abdicating groove to press the cover plate 11, so that the requirement of closing the cover is met. The reaction consumptive material in this embodiment can be arranged in automatic nucleic acid detecting instrument, can realize the automation of reaction consumptive material under automatic nucleic acid detecting instrument's cooperation and open, close the lid, and then has realized the leakproofness in the reaction consumptive material, when satisfying nucleic acid detecting system automation, has also avoided the biological pollution risk among the PCR reaction process.
Example 2:
a consumable PCR material for automated nucleic acid detection, referring to FIG. 4, based on example 1, the difference between this example and example 1 is: the eight tube bodies 2 can be arranged into a multi-connected tube through the connecting plate 6. The connecting plate 6 is rectangular shape, and body 2 inserts and locates connecting plate 6 and the axis perpendicular to connecting plate 6 of body 2, and the periphery lateral wall of going up body 21 is connected with connecting plate 6. Eight bodys 2 are the linear arrangement along the length direction of connecting plate 6, and the mouth of pipe 24 of eight bodys 2 all is in connecting plate 6 with one side and all be in the coplanar. The pressing portions 23 are arranged on the long edges of the connecting plate 6, the long edges of the two sides of the connecting plate 6 are respectively provided with four pressing portions 23, a first yielding groove 61 is formed between every two adjacent pressing portions 23, and the pressing portions 23 on the two sides of the connecting plate 6 in the length direction are in one-to-one correspondence respectively.
Referring to fig. 4, eight caps 1 corresponding to the eight tube bodies 2 are connected by a cap plate 5. The eight tube caps 1 are all located on the same side of the tube cap plate 5, and the axis of the cap body 12 is perpendicular to the tube cap plate 5. The eight tube caps 1 are linearly arranged along the length direction of the tube cap plate 5, and the cap plate 11 and the tube cap plate 5 are integrally formed. The protruding portions 13 are arranged on the long edges of the pipe cover plate 5, the long edges of the two sides of the pipe cover plate 5 are respectively provided with four protruding portions 13, a second yielding groove 51 is formed between every two adjacent protruding portions 13, and the protruding portions 13 on the two sides of the length direction of the pipe cover plate 5 are in one-to-one correspondence respectively. When the tube cap 1 covers the tube body 2, the first yielding grooves 61 correspond to the second yielding grooves 51 one by one.
Referring to fig. 4 and 5, a ring groove is formed in the inner peripheral side wall of the upper tube 21 near the nozzle 24 along the circumferential direction of the upper tube 21 to form a sealing ring 211, and a ring of protruding rings 121 are formed in the outer peripheral side wall of the cover 12 along the circumferential direction of the cover 12. When the tube cap 1 covers and in the tube body 2, the protruding ring 121 is clamped into the sealing ring 211, so that the tightness in the consumable is ensured, and the conditions of nucleic acid pollution or aerosol diffusion and the like in the reaction process are avoided.
Referring to fig. 4, a cylindrical limiting groove 52 is formed in a side surface of the pipe cover plate 5 facing away from the cover body 12, and a radius of the limiting groove 52 is smaller than a radius of the cover body 12. The two limiting grooves 52 are formed and located at two ends of the tube cover plate 5 in the length direction. When the moving member pulls up the tube cover 1, the fixing member on the moving member can be embedded into the limiting groove 52 to fix the tube cover plate 5, so that the tube cover plate 5 is prevented from displacing or falling off from the moving member in the moving process, and subsequent closing and reaction processes are prevented from being influenced.
Referring to fig. 4, the tube body at one end of the connecting plate 6 in the length direction is the special-shaped tube 7, that is, the lower tube 22 and the upper tube 21 are integrally formed into a cylindrical shape, and the bottom of the tube body 2 is a round bottom. The special pipe 7 can be pre-loaded with components such as paraffin oil and the like and is used for being subpackaged into other pipe bodies 2 in the subsequent reaction process so as to reduce the evaporation of liquid in the PCR reaction process and avoid the generation of aerosol pollution.
The working principle is as follows: in the process of pulling up the cap 1, if the moving member in the automated nucleic acid detecting apparatus cannot move directly into the gap between the pressing portion 23 and the protruding portion 13, the moving member may be moved to the first and second avoiding grooves 61 and 51, and then moved into the gap 14 between the pressing portion 23 and the protruding portion 13. The first and second abdicating grooves 61 and 51 can be matched with a moving member of the instrument, so that the moving member can descend or ascend to a specified position at the positions of the first and second abdicating grooves 61 and 51, and can horizontally run at the positions of the first and second abdicating grooves 61 and 51, thereby realizing the operation of opening and closing the cover.
The consumable is designed into a multi-connection pipe so as to meet different requirements for the consumable in PCR reaction and ensure that the consumable has a larger application range.
Example 3:
a consumable PCR material for automated nucleic acid detection, referring to FIG. 6, based on example 2, the difference between this example and example 2 is: the connecting plate 6 is circular, the special pipe 7 is located at the center of the connecting plate 6, the rest seven pipe bodies 2 are distributed at equal intervals along the circumferential direction of the connecting plate 6, the edge of the connecting plate 6 is provided with six first yielding grooves 61, and the six first yielding grooves 61 are distributed at equal intervals along the circumferential direction of the connecting plate 6.
Referring to fig. 6, correspondingly, the tube cover plate 5 is circular, eight tube covers 1 are located on the same side of the tube cover plate 5, one tube cover 1 is located at the center of the tube cover plate 5, and the rest seven tube covers 1 are distributed at equal intervals along the circumferential direction of the tube cover plate 5, so that the tube covers 1 correspond to the tube bodies 2 one to one. Six second abdicating grooves 51 are formed in the edge of the pipe cover plate 5, and the six second abdicating grooves 51 are distributed at equal intervals along the circumferential direction of the pipe cover plate 5.
Set up connecting plate 6 and tube cover plate 5 to circular, can be as required, the instrument of different specifications adopts the consumptive material of different grade type.
The above-mentioned embodiments are merely illustrative of the present invention, and are not intended to limit the present invention, and those skilled in the art can make modifications to the embodiments without inventive contribution as required after reading the present specification, but all the embodiments are protected by patent laws and protection within the scope of the present invention.
Claims (9)
1. The utility model provides a PCR reaction consumptive material for automizing nucleic acid detection, includes body (2), body (2) are opened there is mouth of pipe (24), still includes tube cap (1) that is used for shutoff mouth of pipe (24), its characterized in that:
the outer side wall of the pipe body (2) is provided with a pressing part (23) for fixing the pipe body (2) when the pipe cover (1) is opened;
the pipe cover (1) comprises a cover plate (11) and a cover body (12), wherein the cover plate (11) is provided with a bulge part (13) which is taken as a stress point when the pipe cover (1) is pulled out of the pipe body (2);
when the tube cover (1) covers the tube body (2), a gap is formed between the pressing part (23) and the protruding part (13);
the cover body (12) is provided with a cavity (221) along the length direction of the cover body (12), and a light-passing part for light to enter the pipe body (2) is arranged at the position, corresponding to the cavity (221), of the cover plate (11).
2. The PCR reaction consumable for automated nucleic acid detection according to claim 1, wherein: the pipe body (2) with a plurality of pipe orifices (24) on the same plane is inserted into the connecting plate (6);
the pressing parts (23) are arranged at the edge of the connecting plate (6), and a first yielding groove (61) is formed between every two adjacent pressing parts (23).
3. The PCR reaction consumable for automated nucleic acid detection according to claim 1, wherein: the pipe cover plate is characterized by further comprising a pipe cover plate (5), wherein the bottom surface of the pipe cover plate (5) is provided with a plurality of pipe covers (1) of which the axes are vertical to the pipe cover plate (5);
the protruding parts (13) are arranged on the edge of the pipe cover plate (5), and a second yielding groove (51) is formed between every two adjacent protruding parts (13).
4. The PCR reaction consumable for automated nucleic acid detection according to claim 3, wherein: and one side of the pipe cover plate (5) departing from the pipe cover (1) is provided with a limiting groove (52) for embedding a fixing piece in an instrument to fix the pipe cover plate (5) in the process of horizontally moving the pipe cover plate (5).
5. The PCR reaction consumable for automated nucleic acid detection according to claim 4, wherein: the limiting grooves (52) are provided with at least two.
6. The PCR reaction consumable for automated nucleic acid detection according to claim 1, wherein: the pipe orifice (24) is arranged in a chamfer angle.
7. The PCR reaction consumable for automated nucleic acid detection according to claim 1, wherein: the sealing ring structure is used for increasing the matching sealing performance of the nozzle (24) and the tube cover (1).
8. The PCR reaction consumable for automated nucleic acid detection according to claim 7, wherein: the periphery lateral wall of lid (12) is provided with protruding circle (121), mouth of pipe (24) are provided with and are used for with protruding circle (121) inlay card complex be sunken formula sealing washer (211).
9. The PCR reaction consumable for automated nucleic acid detection according to claim 1, wherein: the light-transmitting part is a light-transmitting part (4) made of polyester material with light-transmitting performance.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921709791.XU CN210886074U (en) | 2019-10-12 | 2019-10-12 | PCR reaction consumable for automatic nucleic acid detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921709791.XU CN210886074U (en) | 2019-10-12 | 2019-10-12 | PCR reaction consumable for automatic nucleic acid detection |
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| Publication Number | Publication Date |
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| CN210886074U true CN210886074U (en) | 2020-06-30 |
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| CN201921709791.XU Active CN210886074U (en) | 2019-10-12 | 2019-10-12 | PCR reaction consumable for automatic nucleic acid detection |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441404A (en) * | 2013-03-14 | 2018-08-24 | 简·探针公司 | Diagnostic system and method |
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2019
- 2019-10-12 CN CN201921709791.XU patent/CN210886074U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108441404A (en) * | 2013-03-14 | 2018-08-24 | 简·探针公司 | Diagnostic system and method |
| CN108441404B (en) * | 2013-03-14 | 2023-09-12 | 简·探针公司 | Diagnostic system and method |
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Address after: Building NE-33, Northeast District, Suzhou Nano City, No. 99 Jinjihu Avenue, Industrial Park, Suzhou City, Jiangsu Province, 215000 Patentee after: SUZHOU TIANLONG BIOTECHNOLOGY Co.,Ltd. Address before: Room 501, Building 07, Northwest District, Suzhou Nano City, No. 99, Jinjihu Avenue, Suzhou Industrial Park, Jiangsu Province, 215000 Patentee before: SUZHOU TIANLONG BIOTECHNOLOGY Co.,Ltd. |