CN211497576U

CN211497576U - Sealing gasket for microfluidic instrument

Info

Publication number: CN211497576U
Application number: CN201921741190.7U
Authority: CN
Inventors: 张四福; 周璟; 邱匀彦; 徐坤; 丁衍超; 徐传来; 马艳红
Original assignee: Suzhou New Geyuan Biotechnology Co ltd
Current assignee: Suzhou New Geyuan Biotechnology Co ltd
Priority date: 2019-10-17
Filing date: 2019-10-17
Publication date: 2020-09-15
Anticipated expiration: 2029-10-17

Abstract

The utility model discloses a sealed pad for micro-fluidic instrument, its technical essential is: the disposable thin pad is characterized by comprising a disposable thin pad, wherein a clamping tenon structure is arranged on the upper surface of the thin pad and used for being detachably assembled with an instrument or a micro-fluidic chip above the thin pad, a through hole is formed in the thin pad in a penetrating mode and used for being connected with an external flow path needle tube, a test solution processing module is connected to the lower side of the thin pad, the test solution processing modules are respectively communicated with the through holes, and a boss corresponding to the through hole is arranged below the thin pad. Is designed in a disposable mode, and has the advantages of preventing pollution and improving sealing effect.

Description

Sealing gasket for microfluidic instrument

Technical Field

The utility model relates to a micro-droplet, micro-fluidic preparation and reagent mix the detection technology field, more specifically says that it relates to a sealed pad for micro-fluidic instrument.

Background

Microfluidic (Microfluidics) technology is one of the important branches of the MEMS technology, and is also one of the advanced technologies of multidisciplinary cross science and technology that is developed rapidly at present, and has important applications in the disciplines of life sciences, clinical medicine, chemical engineering, pharmacy, food sanitation, environmental detection and monitoring, information science, signal detection, and the like.

Microfluidic technology usually uses micro-analysis devices as carriers for technical implementation, and most single-cell analysis needs to separate cells, separate a population of cells into single dispersed cells, and then perform subsequent analysis. Commonly used single cell isolation methods include: multiple dilution of the solution, fluorescence activated cell sorting system, manual separation by microscope operation, laser tweezers separation, and microfluidic separation. Among them, microfluidic separation can achieve high-throughput single-cell separation. Currently, there are mainly two microfluidic-based separation approaches: single cell droplet separation and single cell microwell separation. Compared with a single-cell droplet separation mode, the single-cell micropore separation solves the problem of unstable water-oil droplet formation, high-flux dispersed single cells can be rapidly obtained on a large scale, but the single-cell micropore separation depends on the airtightness control of a multi-hole flow channel of a microfluidic system, and the airtightness degree of the multi-hole flow channel restricts the single-cell micropore separation technology.

The sealing element required by the single-cell micropore separation is a communication medium of the single-cell micropore separation microfluidic main body and an external flow channel, has a complex structure, is high in manufacturing cost and low in assembly yield, and cannot meet the requirements of 100-DEG C high temperature and air tightness in practical application. Meanwhile, the single cell micropore separation technology in the prior art mainly depends on manual operation, and the manual operation of the single cell micropore separation technology has various defects, such as: the manual operation process is easy to make mistakes; the manual operation time is long, and the efficiency is low; the manual preparation depends on operation experience, and the requirement on operators is high; the manual operation can not realize a large amount of sample preparation work; the manually operated sample has the defects of poor cell concentration consistency and the like, and a sealed box body for the single-cell micropore separation technology is developed aiming at the defects, and the sealed box body can carry out an automatic single-cell micropore separation test. Generally, a sealed box for carrying a reagent is assembled with a test instrument, but the bottom of the sealed box is communicated with a plurality of groups of microneedle tubes, so that the problem of reagent leakage exists, and the test instrument which is high in precision and is not cleaned is possibly polluted.

Aiming at the existing single-cell microporous microfluidic separation device, the design of a structure for preventing pollution and improving the sealing property is particularly important for accelerating the optimization test process.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art exists, the utility model aims to provide a sealed pad for micro-fluidic instrument for can abandon the formula design, has the advantage that prevents to pollute, improve sealed effect.

The above object of the utility model is realized through following technical scheme, a sealed pad for micro-fluidic instrument, but thin pad including abandonment formula, thin upper surface of the pad is equipped with the trip structure for instrument or the micro-fluidic chip with the top carry out the detachable equipment, it is equipped with the through-hole to run through on the thin pad, the through-hole supplies external flow path needle tubing to connect, thin pad below is connected test solution processing module, each test solution processing module and respectively in the through-hole intercommunication, thin pad below is equipped with the boss that corresponds with the through-hole position.

Through adopting above-mentioned technical scheme, to various gas circuits, the liquid circuit, reach better seal through above-mentioned structure, guarantee that automatic accomplishing under the condition of uniformity, stabilize the operation process of instrument, through the trip structure, the sealed pad of being convenient for is sealed with the installation and the dismantlement of instrument, realize the sealed cooperation of instrument and micro-fluidic chip module, and trip design convenient to dismantle, disposable sealed pad that has realized abandoning, prevent that the test from producing the pollution, prevent the cross contamination between the sample, the design of bottom boss utilizes the principle of the stress strain of elastomechanics more, increase sealed effect.

The tenon structure is a plurality of groups of limit convex shafts arranged on the periphery of the thin pad.

The shape of the boss is fitted with the shape of the test solution treatment module below.

The through holes are provided with a plurality of groups, and the number of the bosses is equal to that of the through holes.

The size of the through hole is matched with that of the externally-connected flow path needle tube.

By adopting the technical scheme, the thin pad can be made of colloid materials, has better sealing performance, realizes the simultaneous operation of different interfaces by the bosses with various sizes, improves the overall flux, reduces the manufacturing and assembling cost, avoids the difference of batches and improves the stability of samples and test results.

To sum up, the beneficial effects of the utility model are that:

1. the clamping tenon structure is convenient to disassemble, so that the disposable thin pad is realized, and cross contamination is prevented;

2. through the design of the boss of many sizes, be convenient for cooperate multiple interface shape, improve flux and suitability.

Drawings

FIG. 1 is a schematic view showing the overall structure of the present embodiment;

FIG. 2 is a schematic diagram showing a structure of a through hole according to the present embodiment;

fig. 3 is a schematic diagram for showing a tenon structure according to the present embodiment.

Reference numerals: 1. a disposable thin pad; 2. a tenon structure; 3. a through hole; 5. a boss; 6. and limiting the convex shaft.

Detailed Description

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings.

Example (b): as shown in fig. 1, 2 and 3, the sealing gasket for the microfluidic instrument comprises a disposable thin gasket 1, wherein a tenon structure 2 is arranged on the upper surface of the thin gasket and is used for being detachably assembled with an instrument or a microfluidic chip above the thin gasket, a through hole 3 penetrates through the thin gasket, the through hole 3 is connected with an external flow path needle tube, a test solution processing module is connected below the thin gasket and is respectively communicated with the through hole 3, and a boss 5 corresponding to the through hole 3 is arranged below the thin gasket. Aiming at various gas circuits and liquid circuits, better tightness is achieved through the structure, automation is guaranteed to be completed under the condition of consistency, the operation process of an instrument is stabilized, the sealing gasket and the instrument are convenient to install and disassemble through the tenon structure 2, the instrument is in sealing fit with the microfluidic chip module, the tenon is convenient to disassemble, the disposable sealing gasket capable of being discarded is achieved, pollution caused by tests is prevented, cross pollution among samples is prevented, the bottom boss 5 is designed to be the principle of stress-strain of elastomechanics, and the sealing effect is improved.

As shown in fig. 1 and 3, the boss 5 is hidden in fig. 3, the tenon structure 2 of the present embodiment is a plurality of sets of limiting protruding shafts 6 arranged around the thin pad, the number of the limiting protruding shafts 6 can be set and arranged according to the instrument, and the shape of the boss 5 is fitted with the shape of the test solution treatment module below. Through-hole 3 is equipped with the multiunit, boss 5 quantity equals through-hole 3's quantity. The size of the through hole 3 is matched with that of the externally-connected flow path needle tube. The thin pad can be made of colloid materials and has better sealing performance, the bosses 5 attached to the thin pad and in various sizes can realize simultaneous operation of different interfaces, the overall flux is improved, the manufacturing and assembling cost is reduced, the difference of batches is avoided, and the stability of samples and test results is improved.

The present embodiment is only for explaining the present invention, and it is not limited to the present invention, and those skilled in the art can make modifications to the present embodiment without inventive contribution as required after reading the present specification, but all of them are protected by patent laws within the scope of the claims of the present invention.

Claims

1. A gasket for microfluidic instruments, characterized in that: the disposable thin pad is characterized by comprising a disposable thin pad (1), wherein a clamping tenon structure (2) is arranged on the upper surface of the thin pad and is used for being detachably assembled with an instrument or a micro-fluidic chip above the thin pad, a through hole (3) penetrates through the thin pad, the through hole (3) is connected with an external flow path needle tube, a test solution processing module is connected below the thin pad, each test solution processing module is communicated with the through hole (3) respectively, and a boss (5) corresponding to the through hole (3) is arranged below the thin pad.

2. A gasket for microfluidic instruments according to claim 1, characterized in that: the tenon structure (2) is a plurality of groups of limit protruding shafts (6) arranged on the periphery of the thin pad.

3. A gasket for microfluidic instruments according to claim 1, characterized in that: the shape of the boss (5) is fitted with the shape of the test solution treatment module below.

4. A gasket for microfluidic instruments according to claim 1, characterized in that: the through holes (3) are provided with a plurality of groups, and the number of the bosses (5) is equal to that of the through holes (3).

5. A gasket for microfluidic instruments according to claim 1, characterized in that: the size of the through hole (3) is matched with that of the external flow path needle tube.