CN218435664U - PCR amplification container consumable box - Google Patents
PCR amplification container consumable box Download PDFInfo
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- CN218435664U CN218435664U CN202221841418.1U CN202221841418U CN218435664U CN 218435664 U CN218435664 U CN 218435664U CN 202221841418 U CN202221841418 U CN 202221841418U CN 218435664 U CN218435664 U CN 218435664U
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Abstract
The utility model provides a consumable material box of a PCR amplification container, which comprises an amplification card body, a silica gel ball and a medical film, wherein the amplification card body comprises a cylindrical part and a flat part; a side hole is arranged at the end part of the cylindrical part, a liquid feeding hole is arranged at the top part of the cylindrical part, and the side hole is communicated with the liquid feeding hole; the flat part comprises an amplification cavity which is communicated with the side hole through a flow channel; the silica gel ball is arranged in the side hole and is in interference fit with the side hole; the medical film is arranged on two sides of the flat part and is hermetically connected with the flat part. The utility model discloses an amplification card body of constituteing by cylinder portion and platykurtic, set up the liquid feeding hole on the amplification card body, runner and amplification chamber, liquid feeding hole and application of sample suction head phase-match, can satisfy the detection of big flux, avoid cross contamination, the amplification reaction chamber that the thin slice type amplification chamber and film combine to form, temperature rapid change in amplifying for PCR provides good basis, help realizing that PCR nucleic acid detects fast, high flux, help improving automatic application degree, thereby help improving detection efficiency.
Description
Technical Field
The utility model relates to the field of medical equipment, specifically, relate to a PCR amplification container consumptive material box.
Background
Viral detection, such as nucleic acid detection, requires full automation of the process, i.e., sample in and sample out, while at the same time, high demands are placed on large throughput detection, avoiding cross-contamination. At present, amplification consumables adopted by nucleic acid detection at home and abroad basically adopt two types of forms: firstly, 96 orifice plates, eight ally oneself with pipes etc. based on traditional laboratory uses, this type of consumptive material has very big limitation to the mode of heating and cooling of reaction process for the speed of heating and cooling is limited in certain extent. Another class of improvements, such as capillary, flat consumables, while increasing the speed of temperature ramp up and down, do not provide conditions for fully automated applications.
The traditional Chinese patent with the publication number of CN210886074U discloses a PCR reaction consumable for automatic nucleic acid detection, and relates to the field of biological experiment consumables, and the technical scheme of the consumable is characterized by comprising a tube body and a tube cover, wherein the tube body is provided with a tube opening, the tube cover is used for plugging the tube opening, and the outer side wall of the tube body is provided with a pressing part for fixing the tube body when the tube cover is opened; the pipe cover comprises a cover plate and a cover body, and the cover plate is provided with a bulge part which is taken as a stress point when the pipe cover is pulled out of the pipe body; when the tube cover covers the tube body, a gap is formed between the pressing part and the bulge part; the cover body is provided with a cavity along the length direction of the cover body, and the cover plate is provided with a light-through part at the corresponding position of the cavity for light to enter the pipe body.
The inventor considers that the prior art can not simultaneously realize single sample, automatic and continuous rapid temperature rise and fall, meet the detection requirement of high flux and provide the condition of full-automatic application, and needs to provide a more reasonable consumable box of a PCR amplification container.
SUMMERY OF THE UTILITY MODEL
To the defects in the prior art, the utility model aims at providing a PCR amplification container consumptive material box.
According to the utility model, the consumable material box of the PCR amplification container comprises an amplification card body, a silica gel ball and a medical film, wherein the amplification card body comprises a cylindrical part and a flat part; a side hole is formed in the end part of the cylindrical part, a liquid adding hole is formed in the top of the cylindrical part, and the side hole is communicated with the liquid adding hole; the flat part comprises an amplification cavity which is communicated with the side hole through a flow channel; the silica gel ball is placed in the side hole and is in interference fit with the side hole; the medical film is arranged on two sides of the flat part and is in sealing connection with the flat part.
Preferably, the amplification cavity comprises a hollowed circular amplification cavity, and the flow channel comprises a hollowed micro flow channel.
Preferably, the cylindrical portion is disposed horizontally, and the flat portion is disposed directly below the cylindrical portion.
Preferably, the top center of the cylindrical part is provided with a positioning notch.
Preferably, cylinder portion both ends all are provided with the side opening, the side opening includes cylindrical hole, the side opening with the setting of cylinder portion concentric axis.
Preferably, the silica gel ball is placed in any one of the side holes and is positioned between the outer end of the side hole and the liquid adding hole.
Preferably, the silicone ball can move along the direction of the side hole, and when the silicone ball moves to the position of the liquid adding hole, the silicone ball blocks and seals the liquid adding hole and the inlet of the flow channel.
Preferably, the two liquid adding holes are symmetrically arranged on two sides of the top of the cylindrical part, the two flow channels are symmetrically arranged on two sides of the amplification cavity, and the inlets of the flow channels and the liquid adding holes are coaxially arranged.
Preferably, the inlet of the flow channel is horn-shaped, and the inlet of the flow channel gradually increases from the flow channel to the liquid adding hole.
Preferably, the shape of the medical film is matched with the shape of the flat part, and the medical film is bonded or welded with the flat part.
Compared with the prior art, the utility model discloses following beneficial effect has:
1. the utility model discloses an amplification card body of constituteing by cylinder portion and platykurtic, set up the liquid feeding hole on the amplification card body, runner and amplification chamber, liquid feeding hole and application of sample suction head phase-match, can satisfy the detection of big flux, avoid cross contamination, the amplification reaction chamber that the thin slice type amplification chamber and film combine to form, temperature rapid change in amplifying for PCR provides good basis, help realizing that PCR nucleic acid detects fast, high flux, help improving automatic application degree, thereby help improving detection efficiency.
2. The utility model discloses a silica gel ball and side opening interference fit for the side opening can be sealed to the silica gel ball, and after the silica gel ball was impressed the side opening, can seal the entry of liquid feeding hole and runner, help realizing holistic leakproofness, thereby help improving automatic application degree, help improving detection efficiency.
3. The utility model discloses a set up the location breach at cylinder portion top, help on suitable equipment to this motion direction, the accurate positioning of amplification card, help improving automatic application degree, help reducing artifical participation degree to help improving detection efficiency.
Drawings
Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:
FIG. 1 is a schematic view of the overall structure of the consumable box of the PCR amplification container of the present invention;
FIG. 2 is a front view of the consumable box of the PCR amplification container of the present invention;
FIG. 3 is a left side view of the consumable cartridge of the PCR amplification container of the present invention;
FIG. 4 is a right side view of the consumable cassette of the PCR amplification container according to the present invention;
FIG. 5 is a top view of the consumable cartridge of the PCR amplification container of the present invention;
FIG. 6 is a bottom view of the consumable cassette of the PCR amplification container according to the present invention.
Reference numerals:
Positioning notch 15 of liquid feeding hole 14 of side hole 13
Silica gel ball 2 medical film 3
Detailed Description
The present invention will be described in detail with reference to the following embodiments. The following examples will aid those skilled in the art in further understanding the present invention, but are not intended to limit the invention in any way. It should be noted that various changes and modifications can be made by one skilled in the art without departing from the spirit of the invention. These all belong to the protection scope of the present invention.
As shown in FIG. 1, the utility model provides a PCR amplification container consumables box, including: the amplification card body 1 comprises a cylindrical part and a flat part, the silica gel ball 2 and the medical film 3; a side hole 13 is arranged at the end part of the cylindrical part, a liquid feeding hole 14 is arranged at the top part of the cylindrical part, and the side hole 13 is communicated with the liquid feeding hole 14; the flat part comprises an amplification cavity 11, and the amplification cavity 11 is communicated with the side hole 13 through a flow channel 12; the silica gel ball 2 is placed in the side hole 13 and is in interference fit with the side hole; the medical film 3 is arranged on two sides of the flat part and is connected with the flat part in a sealing way.
This application is applicable to nucleic acid detection, can satisfy the requirement that big flux detected, avoided cross contamination, and it is fast to go up and down the temperature in the use, reduces the artifical participation degree of testing process, can effectively improve detection efficiency.
The cylindrical portion is horizontally disposed, and the flat portion is disposed directly below the cylindrical portion. The cylindrical portion and the flat portion of the amplification card body 1 combined, facilitate the directional movement and positioning on the monitoring device. So as to realize the positioning, transmission, centrifugation and automatic sealing of the full-automatic operation process.
Two liquid adding holes 14 are symmetrically arranged at two sides of the top of the cylindrical part, two flow channels 12 are symmetrically arranged at two sides of the amplification cavity 11, and the inlets of the flow channels 12 and the liquid adding holes 14 are arranged in the same central axis. When the reaction liquid is added, only one of the liquid adding holes 14 is used, and the other liquid adding hole 14 is used as an exhaust hole. Reaction liquid's application of sample suction head and liquid feeding hole 14 match each other to because the end of side opening 13 can be sealed to silica gel ball 2, block jam liquid feeding hole 14 inwards along side opening 13, reach the purpose of airtight liquid feeding hole 14, runner 12, reaction liquid adds the in-process and can not outwards leak, can not cause the pollution yet, has effectively guaranteed the degree of accuracy that detects.
The inlet of the flow channel 12 is trumpet-shaped, and the inlet of the flow channel 12 is gradually enlarged from the flow channel 12 to the liquid adding hole 14. In general, the size of the flow channel 12 is smaller than that of the liquid addition hole 14, and a trumpet-shaped transition stage is required to ensure smooth entry of the reaction liquid into the amplification chamber 11.
The amplification chamber 11 may be configured in a variety of shapes, including oval, square, diamond, matching flat sections. Preferably, the amplification chamber 11 is a circular amplification chamber. The flow channels 12 may be provided in a variety of shapes. Preferably, the flow channel 12 is a sheet-type micro flow channel, which can effectively control the flow rate, ensure the smooth flow of the reaction liquid, and improve the detection accuracy.
Further, the amplification chamber 11 may be hollow, hollow or groove-shaped, and the flow channel 12 may also be hollow, hollow or groove-shaped. According to actual detection needs, some detections need heating or cooling, and the liquid in the flat part in the hollow state is separated from the outside by only one medical film 3 serving as a heat-conducting medium, so that the temperature can be rapidly increased or reduced, the detection speed is increased, and the detection in large batch is realized.
The shape of the medical film 3 is matched with that of the flat part, and the medical film 3 is adhered or welded with the flat part to realize the liquid sealing effect.
Furthermore, the position between the two runners 12 on the flat part can be designed in a hollow mode or a hollow mode, so that the weight of the flat part can be effectively reduced, and the production cost can be reduced.
Cylindrical portion top center is provided with location breach 15, will amplify the card body 1 and place back on check out test set, and the axis back-and-forth movement of the cylindrical portion can be followed to the amplification card body 1, and location breach 15 is used for the location when axial motion, the manual operation cost that has significantly reduced.
Both ends of the cylindrical portion are provided with side holes 13, the side holes 13 comprise cylindrical holes, and the side holes 13 and the cylindrical portion are arranged in the same central axis. A silica gel ball 2 is arranged in any side hole 13, and the silica gel ball 2 is positioned between the outer end of the side hole 13 and the liquid feeding hole (14). Preferably, the outer end of the side hole 13 is located on the same vertical plane as the outer end of the silica gel ball 2 placed therein.
The silica gel ball 2 comprises an elastic ball capable of expanding, and the diameter size of the silica gel ball 2 is larger than the aperture size of the side hole 13. In the initial position, the silica gel ball 2 is fixed at the end part of the side hole 13, and the silica gel ball 2 is in a wrapping state and can seal the end part of the side hole 13.
The silicone ball 2 can move along the direction of the side hole 13, and when the silicone ball 2 moves to the position of the liquid adding hole 14, the silicone ball 2 seals the liquid adding hole 14 and the inlet of the flow passage 12. After the amplification card body 1 is placed on the detection equipment, when the amplification card body 1 moves back and forth along the axis of the cylindrical part, the silica gel ball 2 is matched with the ejector rod on the detection equipment, the silica gel ball 2 is pressed into the side hole 13 by the ejector rod, and the silica gel ball 2 expands outwards under the action of tension to be sealed, so that the sealing of the liquid adding hole 14 and the inlet of the flow channel 12 is realized.
When the device is used, reaction liquid is added into one liquid adding hole 14, and after centrifugation is carried out on the device, all the liquid participating in the reaction enters the amplification cavity 11 through the flow channel 12. When the amplification card body 1 moves back and forth along the axis of the cylindrical part, the amplification card body is matched with an ejector rod on equipment, and the silica gel ball 2 is pressed into the bottom of the side hole 13 to plug the inlets of the liquid adding hole 14 and the flow passage 12, so that the liquid in the amplification cavity 11 is sealed.
This application can satisfy automatic application, including automatic liquid feeding, centrifugation, mobile location, automatic gland airtight, the sealed and heat conduction mode of film simultaneously, and to a great extent has improved the quick rise and fall temperature when using. Moreover, the method and the device can effectively reduce the manual participation degree in the detection process, can meet the requirement of large-flux detection, and therefore effectively improve the detection efficiency.
Principle of operation
When the device is used, reaction liquid is added into one liquid adding hole 14, and after centrifugation is carried out on the device, all the liquid participating in the reaction enters the amplification cavity 11 through the flow channel 12. When the amplification card body 1 moves back and forth along the axis of the cylindrical part, the amplification card body is matched with an ejector rod on equipment, and the silica gel ball 2 is pressed into the bottom of the side hole 13, so that the inlets of the liquid adding hole 14 and the flow passage 12 are blocked, and the liquid in the amplification cavity 11 is sealed.
In the description of the present application, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience in describing the present application and simplifying the description, but do not indicate or imply that the referred device or element must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present application.
The foregoing description of the specific embodiments of the invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by those skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (10)
1. A PCR amplification container consumable cassette, comprising: the medical film amplification card comprises an amplification card body (1), a silica gel ball (2) and a medical film (3), wherein the amplification card body (1) comprises a cylindrical part and a flat part;
a side hole (13) is formed in the end part of the cylindrical part, a liquid feeding hole (14) is formed in the top of the cylindrical part, and the side hole (13) is communicated with the liquid feeding hole (14);
the flat part comprises an amplification cavity (11), and the amplification cavity (11) is communicated with the side hole (13) through a flow channel (12);
the silica gel ball (2) is placed in the side hole (13) and is in interference fit with the side hole;
the medical film (3) is arranged on two sides of the flat part and is connected with the flat part in a sealing way.
2. The PCR amplification container consumable cassette of claim 1, wherein the amplification chamber (11) comprises a hollowed circular amplification chamber and the flow channel (12) comprises a hollowed micro flow channel.
3. The PCR amplification vessel consumable cassette of claim 1, wherein the cylindrical portion is disposed horizontally, and the flat portion is disposed directly below the cylindrical portion.
4. The consumable PCR amplification container cassette of claim 1, wherein the top of the cylindrical portion is centrally provided with a positioning notch (15).
5. The PCR amplification vessel consumable cassette of claim 1, wherein the side holes (13) are provided at both ends of the cylindrical portion, the side holes (13) comprise cylindrical holes, and the side holes (13) are concentrically provided with the cylindrical portion.
6. The PCR amplification vessel consumable cassette of claim 5, wherein the silicone ball (2) is disposed in any one of the side holes (13), and the silicone ball (2) is located between the outer end of the side hole (13) and the filling hole (14).
7. The consumable PCR amplification container cassette of claim 1, wherein the silicone ball (2) is capable of moving along the direction of the side hole (13), and when the silicone ball (2) moves to the position of the filling hole (14), the silicone ball (2) blocks and seals the filling hole (14) and the inlet of the flow channel (12).
8. The consumable PCR amplification container cassette according to claim 1, wherein two of the liquid feeding holes (14) are symmetrically disposed at both sides of the top of the cylindrical part, two of the flow channels (12) are symmetrically disposed at both sides of the amplification chamber (11), and the inlet of the flow channel (12) is concentrically disposed with the liquid feeding hole (14).
9. The consumable cassette for PCR amplification vessels according to claim 1, wherein the inlet of the flow channel (12) is flared, and the inlet of the flow channel (12) gradually increases from the flow channel (12) toward the filling hole (14).
10. The PCR amplification container consumable cassette of claim 1, wherein the shape of the medical film (3) matches the shape of the flattened portion, and the medical film (3) is bonded or welded to the flattened portion.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221841418.1U CN218435664U (en) | 2022-07-15 | 2022-07-15 | PCR amplification container consumable box |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221841418.1U CN218435664U (en) | 2022-07-15 | 2022-07-15 | PCR amplification container consumable box |
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| Publication Number | Publication Date |
|---|---|
| CN218435664U true CN218435664U (en) | 2023-02-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221841418.1U Active CN218435664U (en) | 2022-07-15 | 2022-07-15 | PCR amplification container consumable box |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117431157A (en) * | 2023-12-20 | 2024-01-23 | 江苏硕世生物科技股份有限公司 | PCR amplification device |
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2022
- 2022-07-15 CN CN202221841418.1U patent/CN218435664U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117431157A (en) * | 2023-12-20 | 2024-01-23 | 江苏硕世生物科技股份有限公司 | PCR amplification device |
| CN117431157B (en) * | 2023-12-20 | 2024-03-15 | 江苏硕世生物科技股份有限公司 | PCR amplification device |
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